1. A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system
- Author
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Wu Chunfu, Zhang Rong, Zhao Mingyi, Li Wei, Ding Guoyuan, Zou Wenjun, Ma Youlei, Chen Ying, Zhang Jinghai, and Wang Xialu
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Antheraea pernyi ,C-type lectin ,Innate immunity ,Pattern recognition protein ,Pro-PO activation ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
In this paper, we firstly reported a C-type lectin cDNA clone of1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR andRACE techniques. The full-length cDNA contains an openreading frame encoding 308 amino acid residues which hastwo different carbohydrate-recognition domains (CRDs) arrangedin tandem. To investigate the biological activities in theinnate immunity, recombinant Ap-CTL was expressed in E. coliwith a 6-histidine at the amino-terminus (Ap-rCTL). Besidesacted as a broad-spectrum recognition protein binding to awide range of PAMPs and microorganisms, Ap-rCTL also hadthe ability to recognize and trigger the agglutination of bacteriaand fungi. In the proPO activation assay, Ap-rCTL specificallyrestored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs ormicroorganisms. In summary, Ap-rCTL plays an important rolein insect innate immunity as an pattern recognition protein.[BMB Reports 2013; 46(7): 358-363]
- Published
- 2013
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