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A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system
- Source :
- BMB Reports, Vol 46, Iss 7, Pp 358-363 (2013), BMB Reports
- Publication Year :
- 2013
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2013.
-
Abstract
- In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein. [BMB Reports 2013; 46(7): 358-363]
- Subjects :
- China
Recombinant Fusion Proteins
Molecular Sequence Data
Sequence alignment
Antheraea pernyi
Moths
Biochemistry
lcsh:Biochemistry
C-type lectin
Complementary DNA
Hemolymph
Escherichia coli
Animals
Lectins, C-Type
lcsh:QD415-436
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
lcsh:QH301-705.5
Molecular Biology
Peptide sequence
Research Articles
Innate immunity
Phosphotransferases (Phosphate Group Acceptor)
Innate immune system
Base Sequence
biology
business.industry
Pattern recognition
General Medicine
biology.organism_classification
Immunity, Innate
Pattern recognition protein
Open reading frame
Pro-PO activation
lcsh:Biology (General)
Artificial intelligence
business
Sequence Alignment
Protein Binding
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....62eb51468071f9e09f7efa4a5ba1c9d3