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A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system
- Source :
- BMB Reports, Vol 46, Iss 7, Pp 358-363 (2013)
- Publication Year :
- 2013
- Publisher :
- Korean Society for Biochemistry and Molecular Biology, 2013.
-
Abstract
- In this paper, we firstly reported a C-type lectin cDNA clone of1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR andRACE techniques. The full-length cDNA contains an openreading frame encoding 308 amino acid residues which hastwo different carbohydrate-recognition domains (CRDs) arrangedin tandem. To investigate the biological activities in theinnate immunity, recombinant Ap-CTL was expressed in E. coliwith a 6-histidine at the amino-terminus (Ap-rCTL). Besidesacted as a broad-spectrum recognition protein binding to awide range of PAMPs and microorganisms, Ap-rCTL also hadthe ability to recognize and trigger the agglutination of bacteriaand fungi. In the proPO activation assay, Ap-rCTL specificallyrestored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs ormicroorganisms. In summary, Ap-rCTL plays an important rolein insect innate immunity as an pattern recognition protein.[BMB Reports 2013; 46(7): 358-363]
Details
- Language :
- English
- ISSN :
- 19766696 and 1976670X
- Volume :
- 46
- Issue :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- BMB Reports
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.292c14187ad04556b3e47237a8328985
- Document Type :
- article
- Full Text :
- https://doi.org/10.5483/BMBRep.2013.46.7.009