1. Lymphatic absorption of structured triglycerides vs. physical mix in a rat model of fat malabsorption.
- Author
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Tso P, Lee T, and Demichele SJ
- Subjects
- Animals, Cholesterol metabolism, Eicosapentaenoic Acid metabolism, Fish Oils pharmacokinetics, Lymph metabolism, Lymph physiology, Malabsorption Syndromes physiopathology, Male, Phospholipids metabolism, Rats, Rats, Sprague-Dawley, Triglycerides chemistry, Fats pharmacokinetics, Lymphatic System metabolism, Malabsorption Syndromes metabolism, Triglycerides metabolism
- Abstract
Comparison was made between the intestinal absorption and lymphatic transport of a randomly interesterified fish oil and medium-chain triglyceride (MCT) structured triglycerides (STG) vs. the physical mix in rat small intestine following ischemia and reperfusion (I/R) injury. Under halothane anesthesia, the superior mesenteric artery (SMA) was occluded for 20 min and then reperfused in I/R rats. The SMA was isolated but not occluded in control rats. In both treatment groups, the mesenteric lymph duct was cannulated and a gastric tube was inserted. Each treatment group received 1 ml of the fish oil-MCT STG or physical mix (7 rats/group) through the gastric tube followed by an infusion of PBS at 3 ml/h for 8 h. Lymph was collected hourly for 8 h. Lymph triglyceride, cholesterol, and decanoic and eicosapentaenoic acids increased rapidly and maintained a significantly higher output (P < 0.01) with STG compared with physical mix in control rats over 8 h. After I/R, lymphatic triglyceride output decreased 50% compared with control. Gastric infusion of STG significantly improved lipid transport by having a twofold higher triglyceride, cholesterol, and decanoic and eicosapentaenoic acids output to lymph compared with its physical mix (P < 0.01). We conclude that STG is absorbed into lymph significantly better than physical mix by both the normal intestine and the intestine injured by I/R.
- Published
- 1999
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