1. Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin
- Author
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Marc Vendrell, Young-Tae Chang, Duanting Zhai, Jun Cheng Er, and Mui Kee Tang
- Subjects
Models, Molecular ,Drug ,media_common.quotation_subject ,High-throughput screening ,Pharmacokinetics ,medicine ,Humans ,Multiplex ,Serum Albumin ,Fluorescent Dyes ,media_common ,Binding Sites ,Molecular Structure ,Drug discovery ,Chemistry ,General Chemistry ,General Medicine ,Human serum albumin ,Site mapping ,Fluorescence ,High-Throughput Screening Assays ,body regions ,Pharmaceutical Preparations ,Biochemistry ,embryonic structures ,medicine.drug - Abstract
Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.
- Published
- 2013