160 results on '"O'Donnell, James S."'
Search Results
2. Glycolytic reprogramming fuels myeloid cell-driven hypercoagulability
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Rehill, Aisling M., Leon, Gemma, McCluskey, Sean, Schoen, Ingmar, Hernandez-Santana, Yasmina, Annett, Stephanie, Klavina, Paula, Robson, Tracy, Curtis, Annie M., Renné, Thomas, Hussey, Seamus, O’Donnell, James S., Walsh, Patrick T., and Preston, Roger J.S.
- Abstract
Myeloid cell metabolic reprogramming is a hallmark of inflammatory disease; however, its role in inflammation-induced hypercoagulability is poorly understood.
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- 2024
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3. Unraveling coagulation factor–mediated cellular signaling
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O’Donnell, James S., Fleming, Harry, Noone, David, and Preston, Roger J.S.
- Abstract
Blood coagulation is initiated in response to blood vessel injury or proinflammatory stimuli, which activate coagulation factors to coordinate complex biochemical and cellular responses necessary for clot formation. In addition to these critical physiologic functions, plasma protein factors activated during coagulation mediate a spectrum of signaling responses via receptor-binding interactions on different cell types. In this review, we describe examples and mechanisms of coagulation factor signaling. We detail the molecular basis for cell signaling mediated by coagulation factor proteases via the protease-activated receptor family, considering new insights into the role of protease-specific cleavage sites, cofactor and coreceptor interactions, and distinct signaling intermediate interactions in shaping protease-activated receptor signaling diversity. Moreover, we discuss examples of how injury-dependent conformational activation of other coagulation proteins, such as fibrin(ogen) and von Willebrand factor, decrypts their signaling potential, unlocking their capacity to contribute to aberrant proinflammatory signaling. Finally, we consider the role of coagulation factor signaling in disease development and the status of pharmacologic approaches to either attenuate or enhance coagulation factor signaling for therapeutic benefit, emphasizing new approaches to inhibit deleterious coagulation factor signaling without impacting hemostatic activity.
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- 2023
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4. Heterogeneity in the half-life of factor VIII concentrate in patients with hemophilia A is due to variability in the clearance of endogenous von Willebrand factor
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Elsheikh, Einas, Lavin, Michelle, Heck, Lilian Antunes, Larkin, Niamh, Mullaney, Brendan, Doherty, Dearbhla, Kennedy, Megan, Keenan, Catriona, Guest, Thomas, O'Mahony, Brian, Fazavana, Judicael, Fallon, Padraic G., Preston, Roger J.S., Gormley, John, Ryan, Kevin, O'Connell, Niamh M., Singleton, Evelyn, Byrne, Mary, McGowan, Mark, Roche, Sheila, Doyle, Mairead, Crowley, Maeve P., O'Shea, Susan I., Reipert, Birgit M., Johnsen, Jill M., Pipe, Steven W., Di Paola, Jorge, Turecek, Peter L., and O'Donnell, James S.
- Abstract
Previous studies have reported marked interindividual variation in factor VIII (FVIII) clearance in patients with hemophilia (PWH) and proposed a number of factors that influence this heterogeneity.
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- 2023
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5. Variability in International Society on Thrombosis and Haemostasis-Scientific and Standardization Committee endorsed Bleeding Assessment Tool (ISTH-BAT) score with normal aging in healthy females: contributory factors and clinical significance
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Doherty, Dearbhla, Grabell, Julie, Christopherson, Pamela A., Montgomery, Robert R., Coller, Barry S., Lavin, Michelle, O’Donnell, James S., and James, Paula D.
- Abstract
Bleeding assessment tools are key screening tests used in the evaluation of patients with suspected inherited bleeding disorders. The International Society on Thrombosis and Haemostasis-Scientific and Standardization Committee endorsed Bleeding Assessment Tool (ISTH-BAT) has differing reference ranges for adult males (0-3), adult females (0-5), and children (0-2), reflecting differing bleeding symptoms and exposure to hemostatic challenges in these healthy population subgroups. Age is known to markedly impact bleeding score in individuals with von Willebrand disease. However, the influence of age on bleeding score in healthy adult controls is poorly understood.
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- 2023
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6. Macrophage Galactose Lectin Contributes to the Regulation of FVIII (Factor VIII) Clearance in Mice—Brief Report
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Ward, Soracha E., Guest, Thomas, Byrne, Ciara, Lopes, Patricia, O’Sullivan, Jamie M., Doherty, Dearbhla, O’Connell, David, Gutierrez Llaneza, Sara, Chion, Alain, Fazavana, Judicael, Fallon, Padraic G., Preston, Roger J.S., Johnsen, Jill M., Pipe, Steven W., Turecek, Peter L., and O’Donnell, James S.
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- 2023
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7. Enhanced VWF clearance in low VWF pathogenesis: limitations of the VWFpp/VWF:Ag ratio and clinical significance
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Doherty, Dearbhla, Lavin, Michelle, Byrne, Mary, Nolan, Margaret, O’Sullivan, Jamie M., Ryan, Kevin, O’Connell, Niamh M., Haberichter, Sandra L., Christopherson, Pamela A., Di Paola, Jorge, James, Paula D., and O’Donnell, James S.
- Abstract
Increased von Willebrand factor (VWF) clearance plays a key role in the pathogenesis of type 1 and type 2 von Willebrand disease (VWD). However, the pathological mechanisms involved in patients with mild to moderate reductions in plasma VWF:Ag (range, 30-50 IU/dL; low VWF) remain poorly understood. In this study, we investigated the hypothesis that enhanced VWF clearance may contribute to the pathobiology of low VWF. Patients with low VWF were recruited to the LoVIC study after ethics approval and receipt of informed consent. Desmopressin was administered IV in 75 patients, and blood samples were drawn at baseline and at the 1-hour and 4-hour time points. As defined by recent ASH/ISTH/NHF/WFH guidelines, 20% of our low-VWF cohort demonstrated significantly enhanced VWF clearance. Importantly, from a clinical perspective, this enhanced VWF clearance was seen after desmopressin infusion, but did not affect the steady-state VWF propeptide (VWFpp)-to-VWF antigen (VWF:Ag) ratio (VWFpp/VWF:Ag) in most cases. The discrepancy between the VWFpp/VWF:Ag ratio and desmopressin fall-off rates in patients with mild quantitative VWD may have reflected alteration in VWFpp clearance kinetics. Finally, bleeding scores were significantly lower in patients with low VWF with enhanced VWF clearance, compared with those in whom reduced VWF biosynthesis represented the principle pathogenic mechanism. This trial was registered at http://www.clinicaltrials.gov as #NCT03167320.
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- 2023
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8. Enhanced VWF clearance in low VWF pathogenesis: limitations of the VWFpp/VWF:Ag ratio and clinical significance
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Doherty, Dearbhla, Lavin, Michelle, Byrne, Mary, Nolan, Margaret, O’Sullivan, Jamie M., Ryan, Kevin, O’Connell, Niamh M., Haberichter, Sandra L., Christopherson, Pamela A., Di Paola, Jorge, James, Paula D., O’Donnell, James S., Montgomery, R., Flood, V., Haberichter, S., Abshire, T., Weiler, H., Lillicrap, D., James, P., O’Donnell, J., Ng, C., Di Paola, J., Sadler, B., Abshire, T., Bennett, C., Sidonio, R., Manco-Johnson, M., Di Paola, J., Ng, C., Journeycake, J., Zia, A., Lusher, J., Rajpurkar, M., Shapiro, A., Lentz, S., Gill, J., Flood, V., Leissinger, C., Ragni, M., Tarantino, M., Roberts, J., and James, P.
- Abstract
•Subtle enhanced VWF clearance is present in 20% of patients with low VWF and is associated with an attenuated bleeding phenotype.•In patients with low VWF, there is poor correlation between desmopressin fall-off rates and steady-state VWFpp/VWF:Ag ratios.
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- 2023
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9. Low von Willebrand Disease: A Bleeding Disorder of Unknown Cause?
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O'Donnell, James S. and Baker, Ross I.
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- 2023
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10. Perspective: The Case for Acute Large Vessel Ischemic Stroke in COVID-19 Originating Within Thrombosed Pulmonary Venules.
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Meaney, James F.M., O'Donnell, James S., Bridgewood, Charles, Harbison, Joseph, and McGonagle, Dennis
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- 2022
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11. Sustained VWF‐ADAMTS‐13axis imbalance and endotheliopathy in long COVIDsyndrome is related to immune dysfunction
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Fogarty, Helen, Ward, Soracha E., Townsend, Liam, Karampini, Ellie, Elliott, Stephanie, Conlon, Niall, Dunne, Jean, Kiersey, Rachel, Naughton, Aifric, Gardiner, Mary, Byrne, Mary, Bergin, Colm, O'Sullivan, Jamie M., Martin‐Loeches, Ignacio, Nadarajan, Parthiban, Bannan, Ciaran, Mallon, Patrick W., Curley, Gerard F., Preston, Roger J. S., Rehill, Aisling M., Baker, Ross I., Cheallaigh, Cliona Ni, O'Donnell, James S., O’Connell, Niamh, Ryan, Kevin, Kenny, Dermot, and Fazavana, Judicael
- Abstract
Prolonged recovery is common after acute SARS‐CoV‐2 infection; however, the pathophysiological mechanisms underpinning Long COVID syndrome remain unknown. VWF/ADAMTS‐13 imbalance, dysregulated angiogenesis, and immunothrombosis are hallmarks of acute COVID‐19. We hypothesized that VWF/ADAMTS‐13 imbalance persists in convalescence together with endothelial cell (EC) activation and angiogenic disturbance. Additionally, we postulate that ongoing immune cell dysfunction may be linked to sustained EC and coagulation activation. Fifty patients were reviewed at a minimum of 6 weeks following acute COVID‐19. ADAMTS‐13, Weibel Palade Body (WPB) proteins, and angiogenesis‐related proteins were assessed and clinical evaluation and immunophenotyping performed. Comparisons were made with healthy controls (n= 20) and acute COVID‐19 patients (n= 36). ADAMTS‐13 levels were reduced (p= 0.009) and the VWF‐ADAMTS‐13 ratio was increased in convalescence (p= 0.0004). Levels of platelet factor 4 (PF4), a putative protector of VWF, were also elevated (p= 0.0001). A non‐significant increase in WPB proteins Angiopoietin‐2 (Ang‐2) and Osteoprotegerin (OPG) was observed in convalescent patients and WPB markers correlated with EC parameters. Enhanced expression of 21 angiogenesis‐related proteins was observed in convalescent COVID‐19. Finally, immunophenotyping revealed significantly elevated intermediate monocytes and activated CD4+ and CD8+ T cells in convalescence, which correlated with thrombin generation and endotheliopathy markers, respectively. Our data provide insights into sustained EC activation, dysregulated angiogenesis, and VWF/ADAMTS‐13 axis imbalance in convalescent COVID‐19. In keeping with the pivotal role of immunothrombosis in acute COVID‐19, our findings support the hypothesis that abnormal T cell and monocyte populations may be important in the context of persistent EC activation and hemostatic dysfunction during convalescence.
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- 2022
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12. Breast cancer cells mediate endothelial cell activation, promoting von Willebrand factor release, tumor adhesion, and transendothelial migration
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Dhami, Sukhraj Pal Singh, Patmore, Sean, Comerford, Claire, Byrne, Ciara M., Cavanagh, Brenton, Castle, John, Kirwan, Cliona C., Kenny, Martin, Schoen, Ingmar, O'Donnell, James S., and O'Sullivan, Jamie M.
- Abstract
Breast cancer results in a three‐ to four‐fold increased risk of venous thromboembolism (VTE), which is associated with reduced patient survival. Despite this, the mechanisms underpinning breast cancer‐associated thrombosis remain poorly defined. Tumor cells can trigger endothelial cell (EC) activation resulting in increased von Willebrand factor (VWF) secretion. Importantly, elevated plasma VWF levels constitute an independent biomarker for VTE risk. Moreover, in a model of melanoma, treatment with low molecular weight heparin (LMWH) negatively regulated VWF secretion and attenuated tumor metastasis. To investigate the role of VWF in breast cancer metastasis and examine the effect of LMWH in modulating EC activation and breast tumor transmigration. von Willebrand factor levels were measured by ELISA. Primary ECs were used to assess tumor‐induced activation, angiogenesis, tumor adhesion, and transendothelial migration. Patients with metastatic breast cancer have markedly elevated plasma VWF:Ag levels that also correlate with poorer survival. MDA‐MB‐231 and MCF‐7 breast cancer cells induce secretion of VWF, angiopoietin‐2, and osteoprotegerin from ECs, which is further enhanced by the presence of platelets. Vascular endothelial growth factor‐A (VEGF‐A) plays an important role in modulating breast cancer‐induced VWF release. Moreover, VEGF‐A from breast tumor cells also contributes to a pro‐angiogenic effect on ECs. VWF multimers secreted from ECs, in response to tumor‐VEGF‐A, mediate adhesion of breast tumor cells along the endothelium. LMWH inhibits VWF‐breast tumor adhesion and transendothelial migration. Our findings highlight the significant crosstalk between tumor cells and the endothelium including increased VWF secretion which may contribute to tumor metastasis.
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- 2022
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13. Longitudinal bleeding assessment in von Willebrand disease utilizing an interim bleeding score
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Lavin, Michelle, Christopherson, Pamela, Grabell, Julie, Abshire, Thomas, Flood, Veronica, Haberichter, Sandra L., Lillicrap, David, O'Donnell, James S., Montgomery, Robert R., and James, Paula D.
- Abstract
Assessment of bleeding phenotype is critically important in the diagnosis of von Willebrand disease (VWD). Despite advances in bleeding assessment tools (BATs), standardized tools to evaluate bleeding following diagnosis (interim bleeding) are lacking. We assessed the clinical utility of an interim bleeding protocol in a multicenter, international study involving patients with VWD. The enrolment ISTH BAT formed the original bleeding score (0 BS). At follow‐up, the International Society on Thrombosis and Haemostasis BAT was repeated but included only interval bleeding (Interim BS, 1 BS). Both scores were annualized (0 BS/yr, 1 BS/yr). BS were analyzed by VWD subtype, plasma VWF level, sex, and age. Interim BS discriminated by subtype, with significantly increased 0 BS and 1 BS in patients with type 3 VWD. In patients with type 1 VWD, a positive or negative 0 BS did not predict future bleeding, with similar 1 BS/yr (median 1.0 vs. 0.7, p= .2). Despite significantly higher 0 BS in females with type 1 VWD than males (median 7 vs. 5, p= .0012), 1 BS were not significantly different (median 4 vs. 4, p= .16). While 0 BS were lower in children than adults with type 1 VWD, interim BS were similar (median 5 vs. 3, p= .5; 1BS/yr, median 1 vs. 0.8, p= .7). Interestingly, in those with plasma von Willebrand factor:ristocetin cofactor levels >50 IU/dl, interim BS rates were similar to those 30–50 IU/dl (1 BS/yr 0.8 vs. 1.3, p= .5). This study provides both a new approach to longitudinal bleeding assessment and insights into the evolution of bleeding in VWD.
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- 2022
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14. Sustained VWF-ADAMTS-13 axis imbalance and endotheliopathy in long COVID syndrome is related to immune dysfunction
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Fogarty, Helen, Ward, Soracha E., Townsend, Liam, Karampini, Ellie, Elliott, Stephanie, Conlon, Niall, Dunne, Jean, Kiersey, Rachel, Naughton, Aifric, Gardiner, Mary, Byrne, Mary, Bergin, Colm, O'Sullivan, Jamie M., Martin-Loeches, Ignacio, Nadarajan, Parthiban, Bannan, Ciaran, Mallon, Patrick W., Curley, Gerard F., Preston, Roger J.S., Rehill, Aisling M., Baker, Ross I., Cheallaigh, Cliona Ni, O'Donnell, James S., O’Connell, Niamh, Ryan, Kevin, Kenny, Dermot, and Fazavana, Judicael
- Abstract
Prolonged recovery is common after acute SARS-CoV-2 infection; however, the pathophysiological mechanisms underpinning Long COVID syndrome remain unknown. VWF/ADAMTS-13 imbalance, dysregulated angiogenesis, and immunothrombosis are hallmarks of acute COVID-19. We hypothesized that VWF/ADAMTS-13 imbalance persists in convalescence together with endothelial cell (EC) activation and angiogenic disturbance. Additionally, we postulate that ongoing immune cell dysfunction may be linked to sustained EC and coagulation activation.
- Published
- 2022
- Full Text
- View/download PDF
15. Longitudinal bleeding assessment in von Willebrand disease utilizing an interim bleeding score
- Author
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Lavin, Michelle, Christopherson, Pamela, Grabell, Julie, Abshire, Thomas, Flood, Veronica, Haberichter, Sandra L., Lillicrap, David, O'Donnell, James S., Montgomery, Robert R., and James, Paula D.
- Abstract
Assessment of bleeding phenotype is critically important in the diagnosis of von Willebrand disease (VWD). Despite advances in bleeding assessment tools (BATs), standardized tools to evaluate bleeding following diagnosis (interim bleeding) are lacking.
- Published
- 2022
- Full Text
- View/download PDF
16. Breast cancer cells mediate endothelial cell activation, promoting von Willebrand factor release, tumor adhesion, and transendothelial migration
- Author
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Dhami, Sukhraj Pal Singh, Patmore, Sean, Comerford, Claire, Byrne, Ciara M., Cavanagh, Brenton, Castle, John, Kirwan, Cliona C., Kenny, Martin, Schoen, Ingmar, O'Donnell, James S., and O'Sullivan, Jamie M.
- Abstract
Breast cancer results in a three‐ to four‐fold increased risk of venous thromboembolism (VTE), which is associated with reduced patient survival. Despite this, the mechanisms underpinning breast cancer‐associated thrombosis remain poorly defined. Tumor cells can trigger endothelial cell (EC) activation resulting in increased von Willebrand factor (VWF) secretion. Importantly, elevated plasma VWF levels constitute an independent biomarker for VTE risk. Moreover, in a model of melanoma, treatment with low molecular weight heparin (LMWH) negatively regulated VWF secretion and attenuated tumor metastasis.
- Published
- 2022
- Full Text
- View/download PDF
17. Nanoparticle Biomolecular Corona-Based Enrichment of Plasma Glycoproteins for N‑Glycan Profiling and Application in Biomarker Discovery.
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Trinh, Duong N., Gardner, Richard A., Franciosi, Alessandro N., McCarthy, Cormac, Keane, Michael P., Soliman, Mahmoud G., O'Donnell, James S., Meleady, Paula, Spencer, Daniel I. R., and Monopoli, Marco P.
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- 2022
- Full Text
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18. Perspective: The Case for Acute Large Vessel Ischemic Stroke in COVID-19 Originating Within Thrombosed Pulmonary Venules
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Meaney, James F.M., O’Donnell, James S., Bridgewood, Charles, Harbison, Joseph, and McGonagle, Dennis
- Abstract
The main burden of SARS-CoV-2 falls on the lungs but neurological manifestations, the most disabling of which are strokes and which correlate with disease severity, are common. We proffer a novel mechanism for acute COVID-19 stroke whereby pulmonary vein clots developing within the characteristic pulmonary intravascular thrombotic lesions can embolize to the brain. Appreciation of this mechanism requires an understanding of the tricompartmental model of lung parenchyma oxygenation (the alveolus, the bronchial artery, and the pulmonary artery), all of which are compromised in COVID-19. Of these 3 sources, the bronchial artery plays a crucial role in COVID-19 stroke because the unique collaterals from bronchial artery to pulmonary vein which exist under normal physiological conditions (and which maintain venous patency when the pulmonary artery is blocked by embolus) are occluded, thus leading to venular thrombosis in the presence of hypercoagulability. Dislodgement of clots from this source translocates the pathology to the brain and is a disease mechanism, formerly rare, which may account for many cases of large vessel occlusion stroke in COVID-19. This mechanism extends the concept of cardioembolic stroke from endocardium retrogradely into the pulmonary circulation with which the left cardiac chambers lie in direct continuity, and which is an accepted stroke mechanism under other circumstances such as lung lobectomy, where surgical ligation of the pulmonary vein creates a blind sac from which thrombi can embolize. The proposed model is supported by postmortem studies which have demonstrated venular thrombosis and by case reports of pulmonary vein thrombosis in COVID-19. This concept provides a more plausible cause for COVID-19 associated large vessel occlusion stroke than other putative mechanisms, such as cerebral endotheliitis, cytokine storm, and hypercoagulopathy, although it is acknowledged that the latter mechanism contributes to the genesis of pulmonary vein clots. Recognizing that extrapulmonary manifestations including stroke arise within thrombosed pulmonary veins is key to understanding of neurological manifestations of SARS-CoV-2 infection.
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- 2022
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19. Laboratory assays of VWF activity and use of desmopressin trials in the diagnosis of VWD: a systematic review and meta-analysis
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Kalot, Mohamad A., Husainat, Nedaa, Abughanimeh, Omar, Diab, Osama, El Alayli, Abdallah, Tayiem, Sammy, Madoukh, Bader, Dimassi, Ahmad, Qureini, Aref, Ameer, Barbara, Eikenboom, Jeroen, Giraud, Nicolas, Haberichter, Sandra, Jacobs-Pratt, Vicky, Konkle, Barbara A., McRae, Simon, Montgomery, Robert, O’Donnell, James S., Brignardello-Petersen, Romina, Flood, Veronica, Connell, Nathan T., James, Paula, and Mustafa, Reem A.
- Abstract
von Willebrand Disease (VWD) is associated with significant morbidity because of excessive bleeding. Early diagnosis and treatment are important to prevent and treat these symptoms. We systematically reviewed the accuracy of any von Willebrand factor (VWF) activity assay in the diagnosis and classification of patients for VWD. We searched Cochrane Central, MEDLINE, and EMBASE for eligible studies. The risk of bias was assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS)-2 and the certainty of evidence using the GRADE framework. We pooled estimates of sensitivity and specificity. The review included 77 studies that evaluated the use of newer tests of VWF platelet binding activity (VWF:GPIbR, VWF:GPIbM) and VWF:RCo for the diagnosis of VWD (13 studies), VWF propeptide to VWF:Ag ratio, and desmopressin trial for the diagnosis of type 1C VWD (5 studies), VWF multimer analysis and VWF:CB/VWF:Ag ratio for the classification of type 2 VWD (11 studies), genetic testing and ristocetin-induced platelet aggregation to diagnose type 2B VWD (14 studies), genetic testing and FVIII:VWF binding to diagnose type 2N VWD (17 studies). Based on available diagnostic test accuracy, there appear to be comparable test accuracy results between newer tests of platelet binding activity of VWF function and VWF:RCo. The findings of these reviews support VWF multimer analysis or VWF:CB/VWF:Ag to diagnose type 2 VWD. The desmopressin trial test with 1- and 4-hour postinfusion blood work is the test of choice to confirm increased VWF clearance in patients with suspected VWD type 1C. Additionally, genetic testing is most useful in diagnosing type 2B VWD and has a role in the diagnostic algorithm of suspected type 2N VWD.
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- 2022
- Full Text
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20. Laboratory assays of VWF activity and use of desmopressin trials in the diagnosis of VWD: a systematic review and meta-analysis
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Kalot, Mohamad A., Husainat, Nedaa, Abughanimeh, Omar, Diab, Osama, El Alayli, Abdallah, Tayiem, Sammy, Madoukh, Bader, Dimassi, Ahmad, Qureini, Aref, Ameer, Barbara, Eikenboom, Jeroen, Giraud, Nicolas, Haberichter, Sandra, Jacobs-Pratt, Vicky, Konkle, Barbara A., McRae, Simon, Montgomery, Robert, O'Donnell, James S., Brignardello-Petersen, Romina, Flood, Veronica, Connell, Nathan T., James, Paula, and Mustafa, Reem A.
- Abstract
von Willebrand Disease (VWD) is associated with significant morbidity because of excessive bleeding. Early diagnosis and treatment are important to prevent and treat these symptoms. We systematically reviewed the accuracy of any von Willebrand factor (VWF) activity assay in the diagnosis and classification of patients for VWD. We searched Cochrane Central, MEDLINE, and EMBASE for eligible studies. The risk of bias was assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS)-2 and the certainty of evidence using the GRADE framework. We pooled estimates of sensitivity and specificity. The review included 77 studies that evaluated the use of newer tests of VWF platelet binding activity (VWF:GPIbR, VWF:GPIbM) and VWF:RCo for the diagnosis of VWD (13 studies), VWF propeptide to VWF:Ag ratio, and desmopressin trial for the diagnosis of type 1C VWD (5 studies), VWF multimer analysis and VWF:CB/VWF:Ag ratio for the classification of type 2 VWD (11 studies), genetic testing and ristocetin-induced platelet aggregation to diagnose type 2B VWD (14 studies), genetic testing and FVIII:VWF binding to diagnose type 2N VWD (17 studies). Based on available diagnostic test accuracy, there appear to be comparable test accuracy results between newer tests of platelet binding activity of VWF function and VWF:RCo. The findings of these reviews support VWF multimer analysis or VWF:CB/VWF:Ag to diagnose type 2 VWD. The desmopressin trial test with 1- and 4-hour postinfusion blood work is the test of choice to confirm increased VWF clearance in patients with suspected VWD type 1C. Additionally, genetic testing is most useful in diagnosing type 2B VWD and has a role in the diagnostic algorithm of suspected type 2N VWD.
- Published
- 2022
- Full Text
- View/download PDF
21. Nanoparticle Biomolecular Corona-Based Enrichment of Plasma Glycoproteins for N-Glycan Profiling and Application in Biomarker Discovery
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Trinh, Duong N., Gardner, Richard A., Franciosi, Alessandro N., McCarthy, Cormac, Keane, Michael P., Soliman, Mahmoud G., O’Donnell, James S., Meleady, Paula, Spencer, Daniel I. R., and Monopoli, Marco P.
- Abstract
Biomolecular corona formation has emerged as a recurring and important phenomenon in nanomedicine that has been investigated for potential applications in disease diagnosis. In this study, we have combined the “personalized protein corona” with the N-glycosylation profiling that has recently gained considerable interest in human plasma biomarker discovery as a powerful early warning diagnostic and patient stratification tool. We envisioned that the protein corona formation could be exploited as an enrichment step that is critically important in both proteomic and proteoglycomic workflows. By using silica nanoparticles, plasma fibrinogen was enriched to a level in which its proteomic and glycomic “fingerprints” could be traced with confidence. Despite being a more simplified glycan profile compared to full plasma, the corona glycan profile revealed a fibrinogen-derived glycan peak that was found to potentially distinguish lung cancer patients from controls in a pilot study.
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- 2022
- Full Text
- View/download PDF
22. Von Willebrand factor-inflammation crosstalk in deep vein thrombosis
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O’Donnell, James S., Byrne, Ciara, and Preston, Roger J.S.
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- 2023
- Full Text
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23. von Willebrand factor levels in the diagnosis of von Willebrand disease: a systematic review and meta-analysis
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Kalot, Mohamad A., Husainat, Nedaa, El Alayli, Abdallah, Abughanimeh, Omar, Diab, Osama, Tayiem, Sammy, Madoukh, Bader, Dimassi, Ahmad B., Qureini, Aref, Ameer, Barbara, Eikenboom, Jeroen C.J., Giraud, Nicolas, McLintock, Claire, McRae, Simon, Montgomery, Robert R., O’Donnell, James S., Scappe, Nikole, Sidonio, Robert F., Brignardello-Petersen, Romina, Flood, Veronica H., Connell, Nathan T., James, Paula D., and Mustafa, Reem A.
- Abstract
von Willebrand disease (VWD) is associated with significant morbidity as a result of excessive mucocutaneous bleeding. Early diagnosis and treatment are important to prevent and treat these symptoms. We systematically reviewed the accuracy of diagnostic tests using different cutoff values of von Willebrand factor antigen (VWF:Ag) and platelet-dependent von Willebrand factor (VWF) activity assays in the diagnosis of VWD. We searched Cochrane Central Register for Controlled Trials, MEDLINE, and Embase databases for eligible studies. We pooled estimates of sensitivity and specificity and reported patient-important outcomes when relevant. This review included 21 studies that evaluated VWD diagnosis. The results showed low certainty in the evidence for a net health benefit from reconsidering the diagnosis of VWD vs removing the disease diagnosis in patients with VWF levels that have normalized with age. For the diagnosis of type 1 VWD, VWF sequence variants were detected in 75% to 82% of patients with VWF:Ag < 0.30 IU/mL and in 44% to 60% of patients with VWF:Ag between 0.30 and 0.50 IU/mL. A sensitivity of 0.90 (95% confidence interval [CI], 0.83-0.94) and a specificity of 0.91 (95% CI, 0.76-0.97) were observed for a platelet-dependent VWF activity/VWF:Ag ratio < 0.7 in detecting type 2 VWD (moderate certainty in the test accuracy results). VWF:Ag and platelet-dependent activity are continuous variables that are associated with an increase in bleeding risk with decreasing levels. This systematic review shows that using a VWF activity/VWF:Ag ratio < 0.7 vs lower cutoff levels in patients with an abnormal initial VWD screen is more accurate for the diagnosis of type 2 VWD.
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- 2022
- Full Text
- View/download PDF
24. von Willebrand factor levels in the diagnosis of von Willebrand disease: a systematic review and meta-analysis
- Author
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Kalot, Mohamad A., Husainat, Nedaa, El Alayli, Abdallah, Abughanimeh, Omar, Diab, Osama, Tayiem, Sammy, Madoukh, Bader, Dimassi, Ahmad B., Qureini, Aref, Ameer, Barbara, Eikenboom, Jeroen C.J., Giraud, Nicolas, McLintock, Claire, McRae, Simon, Montgomery, Robert R., O'Donnell, James S., Scappe, Nikole, Sidonio, Robert F., Brignardello-Petersen, Romina, Flood, Veronica H., Connell, Nathan T., James, Paula D., and Mustafa, Reem A.
- Abstract
von Willebrand disease (VWD) is associated with significant morbidity as a result of excessive mucocutaneous bleeding. Early diagnosis and treatment are important to prevent and treat these symptoms. We systematically reviewed the accuracy of diagnostic tests using different cutoff values of von Willebrand factor antigen (VWF:Ag) and platelet-dependent von Willebrand factor (VWF) activity assays in the diagnosis of VWD. We searched Cochrane Central Register for Controlled Trials, MEDLINE, and Embase databases for eligible studies. We pooled estimates of sensitivity and specificity and reported patient-important outcomes when relevant. This review included 21 studies that evaluated VWD diagnosis. The results showed low certainty in the evidence for a net health benefit from reconsidering the diagnosis of VWD vs removing the disease diagnosis in patients with VWF levels that have normalized with age. For the diagnosis of type 1 VWD, VWFsequence variants were detected in 75% to 82% of patients with VWF:Ag < 0.30 IU/mL and in 44% to 60% of patients with VWF:Ag between 0.30 and 0.50 IU/mL. A sensitivity of 0.90 (95% confidence interval [CI], 0.83-0.94) and a specificity of 0.91 (95% CI, 0.76-0.97) were observed for a platelet-dependent VWF activity/VWF:Ag ratio < 0.7 in detecting type 2 VWD (moderate certainty in the test accuracy results). VWF:Ag and platelet-dependent activity are continuous variables that are associated with an increase in bleeding risk with decreasing levels. This systematic review shows that using a VWF activity/VWF:Ag ratio < 0.7 vs lower cutoff levels in patients with an abnormal initial VWD screen is more accurate for the diagnosis of type 2 VWD.
- Published
- 2022
- Full Text
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25. Examining international practices in the management of pregnant women with von Willebrand disease
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Lavin, Michelle, Sánchez Luceros, Analia, Kouides, Peter, Abdul‐Kadir, Rezan, O’Donnell, James S., Baker, Ross I., Othman, Maha, and Haberichter, Sandra L.
- Abstract
The management of pregnant women with von Willebrand disease (VWD) is complex as physiological pregnancy‐induced increases in plasma von Willebrand factor (VWF) may be blunted or absent. Women with VWD experience a heightened risk of postpartum hemorrhage (PPH) and special consideration must be given regarding neuraxial anesthesia (NA) and the need for prophylaxis at time of delivery. These challenges are compounded by a lack of robust evidence to guide clinical decision‐making. To determine the current international clinical practices in the management of pregnancy for women with VWD, the International Society on Thrombosis and Haemostasis (ISTH) conducted an international survey of health‐care providers (HCP). One hundred thirty‐two respondents from 39 countries were included in the final analysis. Variations in clinical practice were identified in antenatal (monitoring of plasma VWF and ferritin levels), peripartum (optimal plasma VWF target at delivery) and postpartum management (definitions used for PPH and postpartum monitoring). A key area of divergence was suitability for NA for women with type 2 and type 3 VWD, with many respondents advising against the use of NA even with VWF supplementation (29% type 2 VWD, 37% type 3 VWD) but others advising use once plasma VWF activity was >50 IU/dL (57% type 2 VWD; 50% type 3 VWD). This survey highlighted areas of uncertainty surrounding common management issues for pregnant women with VWD. These data underscore the need for international collaborative research efforts focused on peripartum management to improve care for pregnant women with VWD.
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- 2022
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26. Examining international practices in the management of pregnant women with von Willebrand disease
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Lavin, Michelle, Sánchez Luceros, Analia, Kouides, Peter, Abdul‐Kadir, Rezan, O’Donnell, James S., Baker, Ross I., Othman, Maha, and Haberichter, Sandra L.
- Abstract
The management of pregnant women with von Willebrand disease (VWD) is complex as physiological pregnancy‐induced increases in plasma von Willebrand factor (VWF) may be blunted or absent. Women with VWD experience a heightened risk of postpartum hemorrhage (PPH) and special consideration must be given regarding neuraxial anesthesia (NA) and the need for prophylaxis at time of delivery. These challenges are compounded by a lack of robust evidence to guide clinical decision‐making.
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- 2022
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27. Bleeding assessment tools in the diagnosis of VWD in adults and children: a systematic review and meta-analysis of test accuracy
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Kalot, Mohamad A., Husainat, Nedaa, Tayiem, Sammy, El Alayli, Abdallah, Dimassi, Ahmad B., Diab, Osama, Abughanimeh, Omar, Madoukh, Bader, Qureini, Aref, Ameer, Barbara, Di Paola, Jorge, Eikenboom, Jeroen C.J., Jacobs-Pratt, Vicky, McLintock, Claire, Montgomery, Robert, O'Donnell, James S., Sidonio, Robert, Brignardello-Petersen, Romina, Flood, Veronica, Connell, Nathan T., James, Paula D., and Mustafa, Reem A.
- Abstract
Von Willebrand disease (VWD) can be associated with significant morbidity. Patients with VWD can experience bruising, mucocutaneous bleeding, and bleeding after dental and surgical procedures. Early diagnosis and treatment are important to minimize the risk of these complications. Several bleeding assessment tools (BATs) have been used to quantify bleeding symptoms as a screening tool for VWD. We systematically reviewed diagnostic test accuracy results of BATs to screen patients for VWD. We searched Cochrane Central, MEDLINE, and EMBASE for eligible studies, reference lists of relevant reviews, registered trials, and relevant conference proceedings. Two investigators screened and abstracted data. Risk of bias was assessed using the revised tool for the quality assessment of diagnostic accuracy studies and certainty of evidence using the Grading of Recommendations Assessment, Development and Evaluation framework. We pooled estimates of sensitivity and specificity. The review included 7 cohort studies that evaluated the use of BATs to screen adult and pediatric patients for VWD. The pooled estimates for sensitivity and specificity were 75% (95% confidence interval, 66-83) and 54% (29-77), respectively. Certainty of evidence varied from moderate to high. This systematic review provides accuracy estimates for validated BATs as a screening modality for VWD. A BAT is a useful initial screening test to determine who needs specific blood testing. The pretest probability of VWD (often determined by the clinical setting/patient population), along with sensitivity and specificity estimates, will influence patient management.
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- 2021
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28. How I treat bleeding disorder of unknown cause
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Baker, Ross I. and O’Donnell, James S.
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Recent studies have demonstrated that only 30% of patients referred for assessment of a possible bleeding tendency will eventually be diagnosed with a mild bleeding disorder (MBD) such as von Willebrand disease (VWD) or platelet function defect (PFD). Rather, most of these patients will be diagnosed with bleeding disorder of unknown cause (BDUC). There remains an important unmet need to define consensus regarding the clinical and laboratory criteria necessary for a formal BDUC diagnosis. Accumulating recent data suggest that BDUC is being diagnosed with increasing frequency. Objective assessment of bleeding phenotype using a standardized bleeding assessment tool (BAT) therefore represents a fundamental first step in the diagnosis of BDUC. Because BDUC is a diagnosis by exclusion, accurate quantification of bleeding phenotype is critical because this will be the primary determinant on which a diagnosis of BDUC is reached. Importantly, BAT scores suggest that patients with BDUC display bleeding phenotypes comparable to those seen in patients with VWD or PFD. Despite the prevalence of BDUC, diagnosis and management of these patients commonly pose significant clinical dilemmas. We consider these challenges in the context of a number of typical case studies, discuss the available evidence, and outline our approach to the management of these patients.
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- 2021
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29. How I treat bleeding disorder of unknown cause
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Baker, Ross I. and O'Donnell, James S.
- Abstract
Recent studies have demonstrated that only 30% of patients referred for assessment of a possible bleeding tendency will eventually be diagnosed with a mild bleeding disorder (MBD) such as von Willebrand disease (VWD) or platelet function defect (PFD). Rather, most of these patients will be diagnosed with bleeding disorder of unknown cause (BDUC). There remains an important unmet need to define consensus regarding the clinical and laboratory criteria necessary for a formal BDUC diagnosis. Accumulating recent data suggest that BDUC is being diagnosed with increasing frequency. Objective assessment of bleeding phenotype using a standardized bleeding assessment tool (BAT) therefore represents a fundamental first step in the diagnosis of BDUC. Because BDUC is a diagnosis by exclusion, accurate quantification of bleeding phenotype is critical because this will be the primary determinant on which a diagnosis of BDUC is reached. Importantly, BAT scores suggest that patients with BDUC display bleeding phenotypes comparable to those seen in patients with VWD or PFD. Despite the prevalence of BDUC, diagnosis and management of these patients commonly pose significant clinical dilemmas. We consider these challenges in the context of a number of typical case studies, discuss the available evidence, and outline our approach to the management of these patients.
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- 2021
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30. Current practice and registration patterns among United Kingdom Haemophilia Centre Doctors’ Organisation centers for patients with unclassified bleeding disorders
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Thomas, Will, Downes, Kate, Evans, Gillian, Gidley, Gillian, Lowe, Gill, MacDonald, Stephen, Obaji, Samya, O’Donnell, James S., Palmer, Ben, Pinto, Fernando, and Desborough, Michael
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Bleeding of unknown cause (BUC) and unclassified bleeding disorders (UBD) are increasingly recognized. There is no guidance on diagnosis and management.
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- 2021
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31. Current practice and registration patterns among United Kingdom Haemophilia Centre Doctors’ Organisation centers for patients with unclassified bleeding disorders
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Thomas, Will, Downes, Kate, Evans, Gillian, Gidley, Gillian, Lowe, Gill, MacDonald, Stephen, Obaji, Samya, O’Donnell, James S., Palmer, Ben, Pinto, Fernando, and Desborough, Michael
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Bleeding of unknown cause (BUC) and unclassified bleeding disorders (UBD) are increasingly recognized. There is no guidance on diagnosis and management. To examine UK haemophilia centre registration patterns and current practice for UBD patients. In a two‐step process, the UK National Haemophilia Database (NHD) was reviewed for registration patterns of UBD patients and a survey of UK haemophilia centers was conducted (January/February 2021) to capture current practice for diagnosis and management of patients with UBD. Overall, registrations with the NHD for UBD patients has sharply risen from 2012 to 2020 and in 2019 accounted for 2.65% of registered patients. For the survey, the response rate was 52/67 (78%). Practice was widely variable; 35/52 (67%) centers register UBD; among these 35 centers, terminology included UBD (28 centers), undiagnosed bleeding disorder (four centers), and BUC (three centers); 34/52 (65%) centers use a formal bleeding assessment tool. For management of dental extraction and high bleeding risk surgery in a fictional UBD patient we found that tranexamic acid was widely used; however, beyond this a variety of hemostatic products were advised including blood products, recombinant factor VIIa/prothrombin complex concentrate, and desmopressin. There was general consensus (≈90%) on avoiding regional anesthesia in pregnancy, but no agreement on the need for fetal precautions to avoid bleeding at delivery (50% would advise these). There was a disparity of opinion on chemical thromboprophylaxis, and management of patients without prior hemostatic challenges and offspring of these patients. This study provides a snapshot of current practice and real‐world data in this area. Future studies need to address the gaps in evidence.
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- 2021
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32. Persistent endotheliopathy in the pathogenesis of long COVID syndrome
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Fogarty, Helen, Townsend, Liam, Morrin, Hannah, Ahmad, Azaz, Comerford, Claire, Karampini, Ellie, Englert, Hanna, Byrne, Mary, Bergin, Colm, O’Sullivan, Jamie M., Martin‐Loeches, Ignacio, Nadarajan, Parthiban, Bannan, Ciaran, Mallon, Patrick W., Curley, Gerard F., Preston, Roger J.S., Rehill, Aisling M., McGonagle, Dennis, Ni Cheallaigh, Cliona, Baker, Ross I., Renné, Thomas, Ward, Soracha E., O’Donnell, James S., O’Connell, Niamh, Ryan, Kevin, Kenny, Dermot, and Fazavana, Judicael
- Abstract
Persistent symptoms including breathlessness, fatigue, and decreased exercise tolerance have been reported in patients after acute SARS‐CoV‐2 infection. The biological mechanisms underlying this “long COVID” syndrome remain unknown. However, autopsy studies have highlighted the key roles played by pulmonary endotheliopathy and microvascular immunothrombosis in acute COVID‐19.
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- 2021
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33. Persistent endotheliopathy in the pathogenesis of long COVID syndrome
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Fogarty, Helen, Townsend, Liam, Morrin, Hannah, Ahmad, Azaz, Comerford, Claire, Karampini, Ellie, Englert, Hanna, Byrne, Mary, Bergin, Colm, O’Sullivan, Jamie M., Martin‐Loeches, Ignacio, Nadarajan, Parthiban, Bannan, Ciaran, Mallon, Patrick W., Curley, Gerard F., Preston, Roger J. S., Rehill, Aisling M., McGonagle, Dennis, Ni Cheallaigh, Cliona, Baker, Ross I., Renné, Thomas, Ward, Soracha E., O’Donnell, James S., O’Connell, Niamh, Ryan, Kevin, Kenny, Dermot, and Fazavana, Judicael
- Abstract
Persistent symptoms including breathlessness, fatigue, and decreased exercise tolerance have been reported in patients after acute SARS‐CoV‐2 infection. The biological mechanisms underlying this “long COVID” syndrome remain unknown. However, autopsy studies have highlighted the key roles played by pulmonary endotheliopathy and microvascular immunothrombosis in acute COVID‐19. To assess whether endothelial cell activation may be sustained in convalescent COVID‐19 patients and contribute to long COVID pathogenesis. Fifty patients were reviewed at a median of 68 days following SARS‐CoV‐2 infection. In addition to clinical workup, acute phase markers, endothelial cell (EC) activation and NETosis parameters and thrombin generation were assessed. Thrombin generation assays revealed significantly shorter lag times (p< .0001, 95% CI −2.57 to −1.02 min), increased endogenous thrombin potential (p= .04, 95% CI 15–416 nM/min), and peak thrombin (p< .0001, 95% CI 39–93 nM) in convalescent COVID‐19 patients. These prothrombotic changes were independent of ongoing acute phase response or active NETosis. Importantly, EC biomarkers including von Willebrand factor antigen (VWF:Ag), VWF propeptide (VWFpp), and factor VIII were significantly elevated in convalescent COVID‐19 compared with controls (p= .004, 95% CI 0.09–0.57 IU/ml; p= .009, 95% CI 0.06–0.5 IU/ml; p= .04, 95% CI 0.03–0.44 IU/ml, respectively). In addition, plasma soluble thrombomodulin levels were significantly elevated in convalescent COVID‐19 (p= .02, 95% CI 0.01–2.7 ng/ml). Sustained endotheliopathy was more frequent in older, comorbid patients, and those requiring hospitalization. Finally, both plasma VWF:Ag and VWFpp levels correlated inversely with 6‐min walk tests. Collectively, our findings demonstrate that sustained endotheliopathy is common in convalescent COVID‐19 and raise the intriguing possibility that this may contribute to long COVID pathogenesis.
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- 2021
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34. ADAMTS13 regulation of VWF multimer distribution in severe COVID‐19
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Ward, Soracha E., Fogarty, Helen, Karampini, Ellie, Lavin, Michelle, Schneppenheim, Sonja, Dittmer, Rita, Morrin, Hannah, Glavey, Siobhan, Ni Cheallaigh, Cliona, Bergin, Colm, Martin‐Loeches, Ignacio, Mallon, Patrick W., Curley, Gerard F., Baker, Ross I., Budde, Ulrich, O’Sullivan, Jamie M., O’Donnell, James S., O’Connell, Niamh, Byrne, Mary, Townsend, Liam, McEvoy, Natalie L., Clarke, Jennifer, Boylan, Maria, Alalqam, Razi, Worrall, Amy P., Kelly, Claire, de Barra, Eoghan, Preston, Roger, and Kenny, Dermot
- Abstract
Consistent with fulminant endothelial cell activation, elevated plasma von Willebrand factor (VWF) antigen levels have been reported in patients with COVID‐19. The multimeric size and function of VWF are normally regulated through A Disintegrin And Metalloprotease with ThrombSpondin Motif type 1 motif, member 13 (ADAMTS‐13)‐‐mediated proteolysis. This study investigated the hypothesis that ADAMTS‐13 regulation of VWF multimer distribution may be impaired in severe acute respiratory syndrome‐coronavirus‐2 (SARS‐CoV‐2) infection contributing to the observed microvascular thrombosis. Patients with COVID‐19 (n= 23) were recruited from the Beaumont Hospital Intensive Care Unit (ICU) in Dublin. Plasma VWF antigen, multimer distribution, ADAMTS‐13 activity, and known inhibitors thereof were assessed. We observed markedly increased VWF collagen‐binding activity in patients with severe COVID‐19 compared to controls (median 509.1 versus 94.3 IU/dl). Conversely, plasma ADAMTS‐13 activity was significantly reduced (median 68.2 IU/dl). In keeping with an increase in VWF:ADAMTS‐13 ratio, abnormalities in VWF multimer distribution were common in patients with COVID‐19, with reductions in high molecular weight VWF multimers. Terminal sialylation regulates VWF susceptibility to proteolysis by ADAMTS‐13 and other proteases. We observed that both N‐ and O‐linked sialylation were altered in severe COVID‐19. Furthermore, plasma levels of the ADAMTS‐13 inhibitors interleukin‐6, thrombospondin‐1, and platelet factor 4 were significantly elevated. These findings support the hypothesis that SARS‐CoV‐2 is associated with profound quantitative and qualitative increases in plasma VWF levels, and a multifactorial down‐regulation in ADAMTS‐13 function. Further studies will be required to determine whether therapeutic interventions to correct ADAMTS‐13‐VWF multimer dysfunction may be useful in COVID‐microvascular thrombosis and angiopathy.
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- 2021
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35. ADAMTS13 regulation of VWF multimer distribution in severe COVID‐19
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Ward, Soracha E., Fogarty, Helen, Karampini, Ellie, Lavin, Michelle, Schneppenheim, Sonja, Dittmer, Rita, Morrin, Hannah, Glavey, Siobhan, Ni Cheallaigh, Cliona, Bergin, Colm, Martin‐Loeches, Ignacio, Mallon, Patrick W., Curley, Gerard F., Baker, Ross I., Budde, Ulrich, O’Sullivan, Jamie M., O’Donnell, James S., O’Connell, Niamh, Byrne, Mary, Townsend, Liam, McEvoy, Natalie L., Clarke, Jennifer, Boylan, Maria, Alalqam, Razi, Worrall, Amy P., Kelly, Claire, de Barra, Eoghan, Preston, Roger, and Kenny, Dermot
- Abstract
Consistent with fulminant endothelial cell activation, elevated plasma von Willebrand factor (VWF) antigen levels have been reported in patients with COVID‐19. The multimeric size and function of VWF are normally regulated through A Disintegrin And Metalloprotease with ThrombSpondin Motif type 1 motif, member 13 (ADAMTS‐13)‐‐mediated proteolysis.
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- 2021
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36. Prolonged elevation of D‐dimer levels in convalescent COVID‐19 patients is independent of the acute phase response
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Townsend, Liam, Fogarty, Helen, Dyer, Adam, Martin‐Loeches, Ignacio, Bannan, Ciaran, Nadarajan, Parthiban, Bergin, Colm, O’Farrelly, Cliona, Conlon, Niall, Bourke, Nollaig M., Ward, Soracha E., Byrne, Mary, Ryan, Kevin, O’Connell, Niamh, O’Sullivan, Jamie M., Ni Cheallaigh, Cliona, and O’Donnell, James S.
- Abstract
Persistent fatigue, breathlessness, and reduced exercise tolerance have been reported following acute COVID‐19 infection. Although immuno‐thrombosis has been implicated in acute COVID‐19 pathogenesis, the biological mechanisms underpinning long COVID remain unknown. We hypothesized that pulmonary microvascular immuno‐thrombosis may be important in this context.
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- 2021
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37. Prolonged elevation of D‐dimer levels in convalescent COVID‐19 patients is independent of the acute phase response
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Townsend, Liam, Fogarty, Helen, Dyer, Adam, Martin‐Loeches, Ignacio, Bannan, Ciaran, Nadarajan, Parthiban, Bergin, Colm, O’Farrelly, Cliona, Conlon, Niall, Bourke, Nollaig M., Ward, Soracha E., Byrne, Mary, Ryan, Kevin, O’Connell, Niamh, O’Sullivan, Jamie M., Ni Cheallaigh, Cliona, and O’Donnell, James S.
- Abstract
Persistent fatigue, breathlessness, and reduced exercise tolerance have been reported following acute COVID‐19 infection. Although immuno‐thrombosis has been implicated in acute COVID‐19 pathogenesis, the biological mechanisms underpinning long COVID remain unknown. We hypothesized that pulmonary microvascular immuno‐thrombosis may be important in this context. One hundred fifty COVID‐19 patients were reviewed at St James's Hospital Dublin between May and September 2020 at a median of 80.5 (range 44–155) days after initial diagnosis. These included patients hospitalized during initial illness (n= 69) and others managed entirely as out‐patients (n= 81). Clinical examination, chest x‐ray, and 6‐min walk tests were performed. In addition, a range of coagulation and inflammatory markers were assessed. Increased D‐dimer levels (>500 ng/ml) were observed in 25.3% patients up to 4 months post‐SARS‐CoV‐2 infection. On univariate analysis, elevated convalescent D‐dimers were more common in COVID‐19 patients who had required hospital admission and in patients aged more than 50 years (p< .001). Interestingly, we observed that 29% (n= 11) of patients with elevated convalescent D‐dimers had been managed exclusively as out‐patients during their illness. In contrast, other coagulation (prothrombin time, activated partial thromboplastin time, fibrinogen, platelet count) and inflammation (C‐reactive protein, interleukin‐6, and sCD25) markers had returned to normal in >90% of convalescent patients. Elucidating the biological mechanisms responsible for sustained D‐dimer increases may be of relevance in long COVID pathogenesis and has implications for clinical management of these patients.
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- 2021
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38. Low VWF – unravelling an enigma wrapped in a conundrum.
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O’Donnell, James S., Baker, Ross I., and Atiq, Ferdows
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The 2021 ASH ISTH NHF WFH guidelines recommendation that patients with VWF levels of 30-50 IU/dL and an increased bleeding phenotype be categorized as type 1 VWD rather than Low VWF has proved controversial. However, in support of that decision, recent data have demonstrated that individuals with partial quantitative VWF deficiency exhibit an age-dependent evolving phenotype and confirmed that Low VWF represents a sub-group within heterogeneous type 1 VWD. Nonetheless, type 1 VWD heterogeneity continues to pose significant diagnostic challenges. In this Forum Article, we address outstanding issues critical to preventing the inappropriate overdiagnosis of type 1 VWD, while maximizing access to healthcare and minimizing diagnostic delays. In addition, we propose an algorithm for type 1 VWD diagnosis. This algorithm pays special attention to individuals with plasma VWF levels in the 30-50 IU/dL range who have no or minimal bleeding history and have not yet been exposed to significant hemostatic challenges.
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- 2024
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39. Aptamer BT200 blocks interaction of K1405-1408 in the VWF-A1 domain with macrophage LRP1.
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Chion, Alain, Byrne, Ciara, Atiq, Ferdows, Doherty, Dearbhla, Aguila, Sonia, Fazavana, Judicael, Lopes, Patricia, Karampini, Ellie, Amin, Aamir, Preston, Roger J.S., Baker, Ross I., McKinnon, Thomas A.J., Zhu, Shuhao, Gilbert, James C., Emsley, Jonas, Jilma, Bernd, and O’Donnell, James S.
- Abstract
•A cluster of four lysine residues (K1405, K1406, K1407 and K1408) in the VWF-A1 domain constitutes a novel critical binding site for macrophage LRP1.•BT200 interaction with the VWF-A1 domain in proximity to this lysine cluster significantly attenuates macrophage LRP1-mediated clearance.
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- 2024
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40. R1205H (Vicenza) causes conformational changes in the von Willebrand factor D′D3 domains and enhances von Willebrand factor binding to clearance receptors LRP1 and SR-AI
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Atiq, Ferdows, Rawley, Orla, O’Sullivan, Jamie M., Özbil, Mehmet, Doherty, Dearbhla, Cooke, Niamh, Terraube, Virginie, Chion, Alain, Amin, Aamir, Hulshof, Anne-Marije, Baci, Bogdan, Byrne, Ciara, Aburawi, Hanan E., Lillicrap, David, and O’Donnell, James S.
- Abstract
von Willebrand factor (VWF)-R1205H variant (Vicenza) results in markedly enhanced VWF clearance in humans that has been shown to be largely macrophage-mediated. However, the biological mechanisms underlying this enhanced clearance remain poorly understood.
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- 2024
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41. EXAMINING VARIABILITY IN THE DIAGNOSIS AND MANAGEMENT OF PEOPLE WITH BLEEDING DISORDERS OF UNKNOWN CAUSE: COMMUNICATION FROM THE ISTH SSC SUBCOMMITTEE ON VON WILLEBRAND FACTOR.
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Kelly, Claire, Thomas, William, Baker, Ross I., O’Donnell, James S., Sanchez-Luceros, Analia, and Lavin, Michelle
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Bleeding disorder of unknown cause (BDUC) is characterised by a bleeding phenotype in the setting of normal haemostatic testing. No standardised diagnostic criteria or treatment algorithms exist for people with BDUC. To address the unmet need, the International Society on Thrombosis and Haemostasis von Willebrand Factor Scientific Subcommittee (ISTH VWF SCC) performed a real-world survey, aimed at addressing knowledge gaps, developing consensus pathways and ultimately improving care.
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- 2024
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42. Standardization of definition and management for bleeding disorder of unknown cause: communication from the SSC of the ISTH
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Baker, Ross I., Choi, Philip, Curry, Nicola, Gebhart, Johanna, Gomez, Keith, Henskens, Yvonne, Heubel-Moenen, Floor, James, Paula, Kadir, Rezan Abdul, Kouides, Peter, Lavin, Michelle, Lordkipanidze, Marie, Lowe, Gillian, Mumford, Andrew, Mutch, Nicola, Nagler, Michael, Othman, Maha, Pabinger, Ingrid, Sidonio, Robert, Thomas, Will, and O’Donnell, James S.
- Abstract
In many patients referred with significant bleeding phenotype, laboratory testing fails to define any hemostatic abnormalities. Clinical practice with respect to diagnosis and management of this patient cohort poses significant clinical challenges. We recommend that bleeding history in these patients should be objectively assessed using the International Society on Thrombosis and Haemostasis (ISTH) bleeding assessment tool. Patients with increased bleeding assessment tool scores should progress to hemostasis laboratory testing. To diagnose bleeding disorder of unknown cause (BDUC), normal complete blood count, prothrombin time, activated partial thromboplastin time, thrombin time, von Willebrand factor antigen, von Willebrand factor function, coagulation factors VIII, IX, and XI, and platelet light transmission aggregometry should be the minimum laboratory assessment. In some laboratories, additional specialized hemostasis testing may be performed to identify other rare causes of bleeding. We recommend that patients with a significant bleeding phenotype but normal laboratory investigations should be registered with a diagnosis of BDUC in preference to other terminology. Global hemostatic tests and markers of fibrinolysis demonstrate variable abnormalities, and their clinical significance remains uncertain. Targeted genomic sequencing examining candidate hemostatic genes has a low diagnostic yield. Underlying BDUC should be considered in patients with heavy menstrual bleeding since delays in diagnosis often extend to many years and negatively impact quality of life. Treatment options for BDUC patients include tranexamic acid, desmopressin, and platelet transfusions.
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- 2024
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43. O-glycan determinants regulate VWF trafficking to Weibel-Palade bodies
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Karampini, Ellie, Doherty, Dearbhla, Bürgisser, Petra E, Garre, Massimiliano, Schoen, Ingmar, Elliott, Stephanie, Bierings, Ruben, and O’Donnell, James S.
- Abstract
1.VWF O-glycans critically influence VWF biosynthesis and trafficking into Weibel-Palade bodies in human endothelial cells.2.O-glycan inhibition leads to VWF A1 domain activation and formation of significantly smaller Weibel-Palade bodies.
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- 2024
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44. Type 1 VWD classification revisited: novel insights from combined analysis of the LoVIC and WiN studies
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Atiq, Ferdows, Blok, Robin, van Kwawegen, Calvin B., Doherty, Dearbhla, Lavin, Michelle, van der Bom, Johanna G., O'Connell, Niamh M., de Meris, Joke, Ryan, Kevin, Schols, Saskia E. M., Byrne, Mary, Heubel-Moenen, Floor C. J. I., van Galen, Karin P. M., Preston, Roger J. S., Cnossen, Marjon H., Fijnvandraat, Karin, Baker, Ross I., Meijer, Karina, James, Paula, Di Paola, Jorge, Eikenboom, Jeroen, Leebeek, Frank W. G., and O'Donnell, James S.
- Abstract
•Age-dependent effects on plasma VWF levels in type 1 VWD define distinct subgroups with important differences in underlying pathogenesis.•Low VWF does not constitute a discrete clinical-pathological entity; rather, it is part of an age-dependent type 1 VWD evolving phenotype.
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- 2024
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45. Management of elective procedures in low von Willebrand factor patients in the LoVIC study
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Doherty, Dearbhla, Lavin, Michelle, O’Sullivan, Jamie M., Ryan, Kevin, O’Connell, Niamh M., Dougall, Alison, Byrne, Mary, Rafferty, Marie, Doyle, Mairead M., Di Paola, Jorge, James, Paula D., and O’Donnell, James S.
- Abstract
Most individuals with mild to moderate reductions in plasma von Willebrand factor (VWF) levels do not demonstrate increased bleeding. However, some patients with plasma VWF levels of 30–50 IU/dl do have a significant bleeding phenotype. Management of these “low VWF” patients, who may have significant bleeding scores >10, around times of elective procedures continues to pose a common clinical challenge because of a lack of evidence.
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- 2021
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46. Management of elective procedures in low von Willebrand factor patients in the LoVIC study
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Doherty, Dearbhla, Lavin, Michelle, O’Sullivan, Jamie M., Ryan, Kevin, O’Connell, Niamh M., Dougall, Alison, Byrne, Mary, Rafferty, Marie, Doyle, Mairead M., Di Paola, Jorge, James, Paula D., and O’Donnell, James S.
- Abstract
Most individuals with mild to moderate reductions in plasma von Willebrand factor (VWF) levels do not demonstrate increased bleeding. However, some patients with plasma VWF levels of 30–50 IU/dl do have a significant bleeding phenotype. Management of these “low VWF” patients, who may have significant bleeding scores >10, around times of elective procedures continues to pose a common clinical challenge because of a lack of evidence. To investigate the safety and efficacy of different periprocedural management options for adult patients with low VWF. Treatment and outcomes were retrospectively reviewed for 160 invasive procedures performed in 60 patients with well characterized low VWF enrolled in the previously described Low Von Willebrand factor Ireland Cohort study. We demonstrate that 1‐desamino‐8‐D‐arginine vasopressin is efficacious in preventing bleeding for both minor or major elective procedures in adult low VWF patients, even in those with significant bleeding histories. In addition, tranexamic acid alone is effective for low VWF patients undergoing nondental minor procedures. Importantly, age‐related increases in plasma VWF:antigen levels above 50 IU/dl were not necessarily associated with complete correction of bleeding phenotype. Procedure‐related bleeding complications were increased in low VWF patients who did not receive any hemostatic cover before their procedure. Elective procedures in adult patients with low VWF should be managed in liaison with a comprehensive care tertiary referral center so that personalized treatment plans may be implemented before all minor or major elective procedures.
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- 2021
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47. von Willebrand disease: proposing definitions for future research
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Connell, Nathan T., James, Paula D., Brignardello-Petersen, Romina, Abdul-Kadir, Rezan, Ameer, Barbara, Arapshian, Alice, Couper, Susie, Di Paola, Jorge, Eikenboom, Jeroen, Giraud, Nicolas, Grow, Jean M., Haberichter, Sandra, Jacobs-Pratt, Vicki, Konkle, Barbara A., Kouides, Peter, Laffan, Michael, Lavin, Michelle, Leebeek, Frank W. G., McLintock, Claire, McRae, Simon, Montgomery, Robert, O'Brien, Sarah H., O'Donnell, James S., Ozelo, Margareth C., Scappe, Nikole, Sidonio, Robert, Tosetto, Alberto, Weyand, Angela C., Kalot, Mohamad A., Husainat, Nedaa, Mustafa, Reem A., and Flood, Veronica H.
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- 2021
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48. von Willebrand disease: proposing definitions for future research
- Author
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Connell, Nathan T., James, Paula D., Brignardello-Petersen, Romina, Abdul-Kadir, Rezan, Ameer, Barbara, Arapshian, Alice, Couper, Susie, Di Paola, Jorge, Eikenboom, Jeroen, Giraud, Nicolas, Grow, Jean M., Haberichter, Sandra, Jacobs-Pratt, Vicki, Konkle, Barbara A., Kouides, Peter, Laffan, Michael, Lavin, Michelle, Leebeek, Frank W.G., McLintock, Claire, McRae, Simon, Montgomery, Robert, O'Brien, Sarah H., O'Donnell, James S., Ozelo, Margareth C., Scappe, Nikole, Sidonio, Robert, Tosetto, Alberto, Weyand, Angela C., Kalot, Mohamad A., Husainat, Nedaa, Mustafa, Reem A., and Flood, Veronica H.
- Published
- 2021
- Full Text
- View/download PDF
49. ASH ISTH NHF WFH 2021 guidelines on the diagnosis of von Willebrand disease
- Author
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James, Paula D., Connell, Nathan T., Ameer, Barbara, Di Paola, Jorge, Eikenboom, Jeroen, Giraud, Nicolas, Haberichter, Sandra, Jacobs-Pratt, Vicki, Konkle, Barbara, McLintock, Claire, McRae, Simon, R. Montgomery, Robert, O’Donnell, James S., Scappe, Nikole, Sidonio, Robert, Flood, Veronica H., Husainat, Nedaa, Kalot, Mohamad A., and Mustafa, Reem A.
- Abstract
von Willebrand disease (VWD) is the most common inherited bleeding disorder known in humans. Accurate and timely diagnosis presents numerous challenges.These evidence-based guidelines of the American Society of Hematology (ASH), the International Society on Thrombosis and Haemostasis (ISTH), the National Hemophilia Foundation (NHF), and the World Federation of Hemophilia (WFH) are intended to support patients, clinicians, and other health care professionals in their decisions about VWD diagnosis.ASH, ISTH, NHF, and WFH established a multidisciplinary guideline panel that included 4 patient representatives and was balanced to minimize potential bias from conflicts of interest. The Outcomes and Implementation Research Unit at the University of Kansas Medical Center (KUMC) supported the guideline-development process, including performing or updating systematic evidence reviews up to 8 January 2020. The panel prioritized clinical questions and outcomes according to their importance for clinicians and patients. The panel used the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach, including GRADE Evidence-to-Decision frameworks, to assess evidence and make recommendations, which were subsequently subject to public comment.The panel agreed on 11 recommendations.Key recommendations of these guidelines include the role of bleeding-assessment tools in the assessment of patients suspected of VWD, diagnostic assays and laboratory cutoffs for type 1 and type 2 VWD, how to approach a type 1 VWD patient with normalized levels over time, and the role of genetic testing vs phenotypic assays for types 2B and 2N. Future critical research priorities are also identified.
- Published
- 2021
- Full Text
- View/download PDF
50. ASH ISTH NHF WFH 2021 guidelines on the diagnosis of von Willebrand disease
- Author
-
James, Paula D., Connell, Nathan T., Ameer, Barbara, Di Paola, Jorge, Eikenboom, Jeroen, Giraud, Nicolas, Haberichter, Sandra, Jacobs-Pratt, Vicki, Konkle, Barbara, McLintock, Claire, McRae, Simon, R. Montgomery, Robert, O'Donnell, James S., Scappe, Nikole, Sidonio, Robert, Flood, Veronica H., Husainat, Nedaa, Kalot, Mohamad A., and Mustafa, Reem A.
- Abstract
von Willebrand disease (VWD) is the most common inherited bleeding disorder known in humans. Accurate and timely diagnosis presents numerous challenges.
- Published
- 2021
- Full Text
- View/download PDF
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