Search

To date, few studies have investigated the association between dietary manganese intake and the risk of hypertension, so the prospective relationship of dietary manganese intake and new-onset hypertension remains uncertain. We aimed to investigate the association between dietary manganese intake and the risk of new-onset hypertension in the general Chinese population. This prospective cohort study included 12,177 participants who were free of hypertension at baseline from China Health and Nutrition Survey (CHNS). Dietary intake was measured by 3 consecutive 24-h dietary recalls combined with a household food inventory. The study outcome was new-onset hypertension, defined as systolic blood pressure ≥140 mm Hg or diastolic blood pressure ≥90 mm Hg or diagnosed by a physician or under antihypertensive treatment during the follow-up. During a median follow-up duration of 6.1 years, 4269 (44.9 per 1000 person-years) participants developed new-onset hypertension. Overall, there was a positive association between dietary manganese intake and new-onset hypertension. The adjusted HRs (95%CIs) of new-onset hypertension were 1.00 (reference), 0.97 (0.87, 1.08), 1.24 (1.10, 1.39) and 1.75 (1.52, 2.01) across the quartiles of dietary manganese intake, respectively. Accordingly, a significantly higher risk of new-onset hypertension (HR, 1.38; 95%CI: 1.27, 1.50) was found in participants in quartiles 3–4 of dietary manganese intake (≥6.0 mg/day), compared with those in quartiles 1–2 (<6.0 mg/day). In the general Chinese population, dietary manganese intake was positively associated with the risk of new hypertension, independent of sodium intake and other important covariates. • This is the first prospective study about the relationship of dietary Mn intake and hypertension. • Dietary Mn intake was positively associated with the risk of hypertension. • Maintaining an optimal Mn intake level could prevent hypertension. [ABSTRACT FROM AUTHOR]

The association between dietary phosphorus intake and the risk of diabetes remains uncertain. We aimed to investigate the relation of dietary phosphorus intake with new-onset diabetes among Chinese adults. A total of 16,272 participants who were free of diabetes at baseline from the China Health and Nutrition Survey were included. Dietary intake was measured by 3 consecutive 24-h dietary recalls combined with a household food inventory. Participants with self-reported physician-diagnosed diabetes, or fasting glucose ≥7.0 mmol/L or glycated hemoglobin ≥6.5% during the follow-up were defined as having new-onset diabetes. During a median follow-up of 9.0years, 1101 participants developed new-onset diabetes. Overall, the association between dietary phosphorus intake with new-onset diabetes followed a U-shape (P for nonlinearity<0.001). The risk of new-onset diabetes significantly decreased with the increment of dietary phosphorus intake (per SD increment: HR, 0.64; 95%CI, 0.48–0.84) in participants with phosphorus intake <921.6 mg/day, and increased with the increment of dietary phosphorus intake (per SD increment: HR, 1.33; 95%CI, 1.16–1.53) in participants with phosphorus intake ≥921.6 mg/day. Consistently, when dietary phosphorus intake was assessed as quintiles, compared with those in the 3rd quintile (905.0-<975.4 mg/day), significantly higher risks of new-onset diabetes were found in participants in the 1st-2nd quintiles (<905.0 mg/day: HR, 1.59; 95%CI, 1.30–1.94), and 4th-5th quintiles (≥975.4 mg/day: HR, 1.46; 95%CI, 1.19–1.78). There was a U-shaped association between dietary phosphorus intake and new-onset diabetes in general Chinese adults, with an inflection point at 921.6 mg/day and a minimal risk at 905.0–975.4 mg/day of dietary phosphorus intake. • There was a U-shaped relation of dietary phosphorus intake with new-onset diabetes. • The inflection point of phosphorus intake was at 921.6 mg/d for new-onset diabetes. • Those with phosphorus intake at 905.0–975.4 mg/d showed the lowest diabetes risk. [ABSTRACT FROM AUTHOR]

The association between dietary phosphorus intake and the risk of hypertension remains uncertain. We aimed to investigate the relation of dietary phosphorus intake with new-onset hypertension among Chinese adults. A total of 12,177 participants who were free of hypertension at baseline from the China Health and Nutrition Survey (CHNS) were included. Dietary intake was measured by 3 consecutive 24-hour dietary recalls combined with a household food inventory. New-onset hypertension was defined as systolic blood pressure ≥140 mm Hg or diastolic blood pressure ≥90 mm Hg or diagnosed by a physician or under antihypertensive treatment during the follow-up. During a median follow-up of 6.1 years, 4,269 participants developed new-onset hypertension. Overall, the association between dietary phosphorus intake and new-onset hypertension followed a U-shape (P for nonlinearity<.001). Consistently, when dietary phosphorus intake was assessed as quintiles, compared with those in the 3rd to 4th quintiles (912.0-<1089.5 mg/d), a significantly higher risk of new-onset hypertension was found in participants in the 1st to 2nd quintiles (<912.0 mg/d: HR, 1.23; 95% CI, 1.14-1.33), and the fifth quintile (≥1089.5 mg/d: HR, 1.21; 95% CI, 1.10-1.33). There was a U-shaped association between dietary phosphorus intake and new-onset hypertension in general Chinese adults. [ABSTRACT FROM AUTHOR]

To investigate the relationship of dietary copper intake with new-onset hypertension among Chinese adults. A total of 12,245 participants who were free of hypertension at baseline from the China Health and Nutrition Survey (CHNS) were included. Dietary intake was measured by 3 consecutive 24-h dietary recalls combined with a household food inventory. New-onset hypertension was defined as systolic blood pressure ≥140 mmHg and/or diastolic blood pressure ≥90 mmHg or diagnosed by a physician or under antihypertensive treatment during the follow-up. During a median follow-up of 6.1 years, 4304 participants developed new-onset hypertension. Overall, the associations between dietary copper intake and new-onset hypertension followed a U-shape (P for nonlinearity <0.001). The risk of new-onset hypertension significantly decreased with the increment of dietary copper intake (per SD increment: HR, 0.71; 95% CI, 0.57–0.88) in participants with copper intake <1.57 mg/day, and increased with the increment of dietary copper intake (per SD increment: HR, 1.09; 95% CI: 1.07–1.12) in participants with copper intake ≥1.57 mg/day. There was a U-shaped association between dietary copper intake and new-onset hypertension in general Chinese adults, with an inflection point at about 1.57 mg/day. Our results emphasized the importance of maintaining optimal copper intake levels for the primary prevention of hypertension. [ABSTRACT FROM AUTHOR]

Trimethylamine-N-oxide (TMAO), a gut microbiota-produced metabolite, is accumulated in chronic kidney disease (CKD) patients. It is well known to contribute to CKD-related cardiovascular complications. However, the effect of TMAO on peritoneal dialysis (PD)-related peritonitis remains largely unknown. Here, we demonstrate that serum concentrations of TMAO were positively correlated with C-reactive protein levels, and the appearance rate of dialysate IL-6 and PAI-1, in PD patients. During the follow-up period of 28.3 ± 8.0 months, patients with higher TMAO levels (≥50 μM) had a higher risk of new-onset peritonitis (HR, 3.60; 95%CI, 1.18-10.99; P=0.025) after adjusting for sex, age, diabetes, PD duration, BUN, rGFR, C-reactive protein, BMI and β2-M. In CKD rat models, TMAO significantly promoted peritoneal dialysate-induced inflammatory cell infiltration, inflammatory cytokines production in the peritoneum. In vitro study revealed that TMAO directly induced primary peritoneal mesothelial cell necrosis, together with increased production of pro-inflammatory cytokines including CCL2, TNF-α, IL-6, and IL-1β. In addition, TMAO significantly increased TNF-α-induced P-selectin production in mesothelial cells, as well as high glucose-induced TNF-α and CCL2 expression in endothelial cells. In conclusion, our data demonstrate that higher levels of TMAO exacerbate peritoneal inflammation and might be a risk factor of incidence of peritonitis in PD patients. [ABSTRACT FROM AUTHOR]

The impacts of lead/Pb2+ on ecosystems have received widespread attention. Growth suppression is a major toxic effect of Pb compounds on aquatic animals, however, some studies have also reported their growth-promoting effects. These complex outcomes may be explained by anions that accompany Pb2+ or by the multiple toxic mechanisms/pathways of Pb2+. To examine these hypotheses, we tested how Bufo gargarizans tadpoles responded to Pb(NO 3) 2 (100 and 200 μg/L Pb2+) using transcriptomics and microbiomics, with NaNO 3 and blank groups as controls. Tadpoles exposed to Pb(NO 3) 2 showed delayed development while increased somatic growth in a dose-dependent manner, which can be attributed to the effects of NO 3 - and Pb2+, respectively. Tadpole transcriptomics revealed that exposure to NO 3 - downregulated the MAPK pathway at transcriptional level, explaining the development-suppressing effect of NO 3 -; while Pb2+ upregulated the transcription of detoxification pathways (e.g., xenobiotics metabolism by cytochrome P450 and glutathione metabolism), indicating cellular stress and thus contradicting the growth advantage of Pb2+-exposed tadpoles. Pb2+ exposure changed the tadpole gut microbiota drastically, characterized by increased polysaccharides and carbohydrate utilization while decreased fatty acid and amino acid consumption according to microbial functional analysis. Similar gut microbial variations were observed in field-collected tadpoles from different Pb2+ environments. This metabolic shift in gut microbiota likely improved the overall food utilization efficiency and increased the allocation of fatty acids and amino acids to the host, explaining the growth advantage of Pb2+-exposed tadpoles. In summary, our results suggest multiple toxic pathways of Pb2+, and the gut microbiota may affect the pollution outcomes on animals. [Display omitted] • Pb(NO 3) 2 has development-suppressing and growth-promoting effects on tadpoles. • NO 3 - and Pb2+ account for the effects on development and growth, respectively. • NO 3 - inhibits tadpole MAPK pathway and thus causes developmental suppression. • Pb2+ causes cellular stresses in tadpole and changes their gut microbial function. • Gut microbial changes explain the growth-promoting effects of Pb2+ on tadpoles. [ABSTRACT FROM AUTHOR]

Concerns about the implications of microplastics (MPs) on aqueous animals have gained widespread attention. It has been postulated that the magnitude of MPs can influence its toxicity. However, little is known about how MPs toxicity changes with particle size. Amphibians are reliable bioindicators of ecosystem health due to their complex life cycles. In this study, we compared the influences of two sizes nonfunctionalized polystyrene microspheres (1 and 10 μm) on the metamorphosis of Asiatic toad (Bufo gargarizans). Acute exposure to MPs at high concentrations led to bioaccumulation in the digestive track and internal organs (i.e., liver and heart) of tadpoles. Long-term exposure to either size, at environmentally-related concentrations (1 and 4550 p/mL), led to growth and development delay in pro-metamorphic tadpoles. Remarkably, developmental plasticity mitigated these deleterious effects prior to the onset of metamorphic climax without compromising survival rate in later stages. MPs with a diameter of 10 μm dramatically altered the gut microbiota (e.g., abundance of Catabacter and Desulfovibrio) of pro-metamorphic tadpoles, whereas MPs with a diameter of 1 μm induced much more intensive transcriptional responses in the host tissues (e.g., upregulation of protein synthesis and mitochondrial energy metabolism, and downregulation of neural functions and cellular responses). Given that the two MPs sizes induced similar toxic outcomes, this suggests that their principal toxicity mechanisms are distinct. Small-sized MPs can travel easily across the intestinal mucosa and cause direct toxicity, while large-sized MPs accumulate in gut and affect the host by changing the homeostasis of digestive track. In conclusion, our findings indicate that MPs can affect the growth and development of amphibian larvae, but their developmental plasticity determines the ultimate detrimental effects. Multiple pathways of toxicity may contribute to the size-dependent toxicity of MPs. We anticipate that these findings will increase our understanding of the ecological effects of MPs. [Display omitted] • Both 1 and 10 μm MPs affect the growth and development of pro-metamorphic tadpoles. • Developmental plasticity offsets the effects of MPs before metamorphic climax. • Differently-sized MPs impact host tissues and symbiotic microbiota discriminately. • Large-sized MPs mainly affect the diversity and function of gut symbiotic microbiota. • Small-sized MPs mainly target host tissues and affect mitochondrial and neural functions. [ABSTRACT FROM AUTHOR]

Background: Chinese rice wine (CRW) is a well-known drink and functional food that is used in traditional Chinese medicine. However, there is still a lack of quality control and evaluation methods for CRWs.Purpose: The study aimed to establish a new method that can serve both as quality control and evaluation method and, as well as an identification method for CRWs.Method: Compound identification in different CRW samples and determination of uracil, xanthine, uridine, adenine, guanosine, 5-hydroxymethylfurfural, and adenosine contents from 29 CRW samples from 14 brands were performed using UPLC-PDA/TOF-MS. The dual-column chromatographic separation of CRW was performed using CORTECS T3 coupled to HSS T3. The optimal mobile phase consisted of water with 0.1% formic acid, 40 mM ammonium acetate, and methanol: acetonitrile (2:1). Furthermore, to compare the UPLC fingerprints between CRWs of different brands, a similarity analysis was performed to classify the CRW samples. Finally, network pharmacology and in vitro efficacy and toxicity tests were used to investigate the biological function of the seven components and CRWs.Results: A total of 55 compounds were unambiguously or tentatively identified. Among them, nucleoside, pyrimidines and purines were reported in CRW for the first time. The seven components were successfully determined, and their contents showed large variations among different brands of CRW, which was consistent with the results of the chromatographic fingerprint similarities. The results of in vitro efficacy and toxicity tests indicated that CRWs and seven components had obvious protective effect on H9c2 cell injury induced by the H2O2 model. Network pharmacology analysis showed that these seven compounds might be the main active components of CRW that promote blood circulation and ventilation.Conclusion: This study revealed that dual-column chromatographic separation is an effective method for quantitative and chromatographic fingerprint analyzes of complex samples, and seven compounds can be used for the quality evaluation and control of CRWs. [ABSTRACT FROM AUTHOR]

Inhalable particulate matter, especially PM2.5 is one of the main pollutants in China and it's harmful to both human health and atmosphere. Since the removal efficiency of traditional dust removal devices such as ESP for PM2.5 is very low, pretreatment becomes necessary before the dust gets into the dust remover. Acoustic agglomeration is one of the pretreatment technologies which uses sound wave with high intensity to make fine particles get agglomerate and grow up, and improves the efficiency of traditional dust removal devices for PM 2.5 . In sound wave field, fine particles are carried by the medium which in this paper is air, and vibrate with different amplitude because of different particle sizes, thus relative movement appears and then particles have more chances to collide and get agglomerate. In this paper, the movement of particles with different sizes in travelling wave sound field and standing wave sound field were calculated, including the velocity, displacement, amplitude and so on. The situation that Re<1 was considered and Viscous force in Stokes region was chose as the main forces here. Studying the movement of fine particle in sound field with different conditions has great meaning in learning the mechanisms of acoustic agglomeration. [ABSTRACT FROM AUTHOR]

Inhalable particulate matter, especially PM2.5 is one of the main pollutants in China and it's harmful to both human health and atmosphere. Since the removal efficiency of traditional dust removal devices such as ESP for PM2.5 is very low, pretreatment becomes necessary before the dust gets into the dust remover. Acoustic agglomeration is one of the pretreatment technologies which uses sound wave with high intensity to make fine particles get agglomerate and grow up, and improves the efficiency of traditional dust removal devices for PM 2.5 . In sound wave field, fine particles are carried by the medium which in this paper is air, and vibrate with different amplitude because of different particle sizes, thus relative movement appears and then particles have more chances to collide and get agglomerate. In this paper, the movement of particles with different sizes in travelling wave sound field and standing wave sound field were calculated, including the velocity, displacement, amplitude and so on. The situation that Re<1 was considered and Viscous force in Stokes region was chose as the main forces here. Studying the movement of fine particle in sound field with different conditions has great meaning in learning the mechanisms of acoustic agglomeration. [ABSTRACT FROM AUTHOR]

Respirable particulate matter, especially fine particles are doing great harm to human health and the environment nowadays, but the removal efficiency of traditional dust removal devices for PM 2.5 is quite low. Acoustic agglomeration is a pretreatment technology of dust removal which uses sound wave with high intensity to make fine particles get agglomerate and grow up, and improves the efficiency of traditional dust removal devices for PM 2.5 and thus reduces the concentration of fine particle emissions. In this paper, the experimental set-up of acoustic agglomeration is designed and reconstructed. Coal-fired ashes are mixed with air to simulate aerosol emissions from coal-fired power plants. A travelling wave acoustic field is generated in a vertical agglomeration chamber in which the residence time of the aerosol is around 4s. Preliminary experimental study of the influence of acoustic frequency, SPL and the initial concentration of aerosol on the acoustic agglomeration is conducted. It is found that the efficiency of agglomeration is very sensitive to the change of acoustic frequency and there exists an optimal frequency which in this paper is 1400 Hz. The effect of acoustic agglomeration has a linear relationship with SPL and it is enhanced with the increase of SPL. The increase of the initial number concentration of aerosol can also enhance the agglomeration effect, but the rate of the increase of the agglomeration effect decreases while the initial concentration is getting bigger. When the acoustic frequency is 1400 Hz, the SPL is 142 dB and the initial concentration is 4.04×10 5 cm -3 , the number concentration of aerosol can be reduced to 35% of the initial value. [ABSTRACT FROM AUTHOR]

Background: Rhein, an anthraquinone compound, displays extensive antifibrotic effects; however, its potential mechanisms are not fully understood. In this study, we explored the underlying molecular mechanism of action of rhein.Method: An integrated network pharmacology and cell metabolomics approach was developed based on network pharmacology and bioinformatics method, and then successfully applied to speculate the potential targets of rhein and construct a rhein-target-metabolic enzyme-metabolite network. Thereafter, the antifibrotic mechanism of rhein was validated in TGF-β- and oleic acid- induced HK-2 and NRK-52E cells in vitro as well as a unilateral ischemia-reperfusion injury Sprague-Dawley rat model.Results: Based on the construction of the rhein-target-metabolic enzyme-metabolite network, we found that rhein played an antifibrotic role through the PPAR-α-CPT1A-l-palmitoyl-carnitine axis. In vitro experiments demonstrated that rhein effectively activated the expression of PPARα and its downstream proteins (CPT1A and ACOX1) to alleviate lipid accumulation and fibrosis development. In vivo experiments indicated that rhein attenuated renal fibrosis mainly by activating the fatty acid oxidation pathway and improving lipid metabolism.Conclusion: Taken together, our findings reveal that rhein is a novel agonist of PPARα, which contributes to its renoprotection through the regulation of the PPARα-CPT1A axis. Moreover, our study provides a novel insight into an integrated network pharmacology-metabolomics strategy for uncovering the pharmacological mechanisms of drugs from the system perspective. [ABSTRACT FROM AUTHOR]

Abstract: Micro arc oxidation (MAO) coatings were formed on magnesium alloy AZ31B to improve the corrosion resistance using environmental friendly electrolyte solution under single-polar pulse power supply. The effect of electrical parameters of pulse frequency on the coating performance was studied at frequencies of 2.5 Hz, 25 Hz and 250 Hz. The coating performance at different frequencies was characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and potentiodynamic polarization testing method. It was found that the corrosion resistance of the MAO coatings was improved when increasing the pulse frequencies from 2.5 Hz to 250 Hz. The corrosion current in 250 Hz case decreased by three orders of magnitude as compared with bare Mg alloy and the corrosion impedance also increased remarkably, means significantly enhanced corrosion resistance than bare Mg alloy. The results give us more insight in the optimization of electrical parameters to improve the MAO coating performance. The MAO technology is attractive for application on magnesium and other light alloys for surface protection in automotive and space industries. [Copyright &y& Elsevier]

Corticosterone (CORT)-mediated adaptive plasticity improves animal fitness in stressful environments. Although it brings ecological benefits, the cost potentially constrains its expression and evolution. Revealing the factors affecting plasticity costs is of great ecological and evolutionary significance. Evidence indicates that both CORT and background colour can induce metabolic changes in animals, which in turn determine phenotypic plasticity. However, whether and/or how CORT and background colour jointly act on plastic responses has not been studied. Here, this question has been investigated in amphibian tadpoles (Microhyla fissipes) exposed to CORT at different background colours (white or black) using integrated morphological, histological, and transcriptomic analyses. The results showed that CORT exposure increased relative tail length, immune function, and metabolic maintenance (i.e., transcription of substrate catabolism and oxidative phosphorylation) at the expense of reduction in growth rate and skin melanin level. The black background also increased relative tail length and metabolic maintenance (i.e., transcription of oxidative phosphorylation) at the cost of reduction in growth rate, but increased skin melanin level. The expression of critical pigmentation genes indicated that black background activated a distinct and opposite pigmentation regulating route to CORT. Although there was no interactive effect of background colour and CORT on phenotypic and metabolic variations, their additive effects further impact the trade-off between somatic growth, metabolic maintenance, and pigmentation in terms of resource allocation. In conclusion, the individual and additive effects of background colour and CORT exposure on tadpole plasticity were revealed. These results likely provide new insights into the environmental adaptation of animals. [Display omitted] • Corticosterone (CORT) exposure increased metabolic maintenance and immunity. • Black background increased metabolic maintenance and pigmentation. • Both CORT exposure and black background inhibited the somatic growth. • Different combinations of CORT exposure and background colour induced various strategies of resource allocations. [ABSTRACT FROM AUTHOR]

Glyphosate-based herbicides (GBHs) are widely-used agricultural chemicals, bringing potential detriments to aquatic organisms. Currently, our understanding of sublethal effects and underlying toxicologic mechanisms of GBHs are still limited, especially in amphibians. Here, the sublethal effects of a commercial GBH (KISSUN®) on tadpoles of a farmland dwelling frog, Microhyla fissipes , were investigated. The 10-d LC 50 of "KISSUN®" GBH was 77.5 mg/L. Tadpoles exposed to 60–120 mg/L showed increased preference to higher temperature. After 10 days exposure, obvious growth suppression was observed in survived GBH-stressed tadpoles, characterized by dosage depended decrement in body mass, body width, total length, etc. GBH-stressed tadpoles also showed decreased tail length/snout-vent length ratio and smaller tail muscle fiber diameter. Comparative transcriptomics (control, 60 mg/L and 90 mg/L groups) was conducted to analyze the underlying molecular processes. GBH-stressed tadpoles showed downregulated transcription of ribosomal proteins and cytoskeleton proteins, which could explain their suppressed whole body and tail muscle growth. Moreover, GBH-stressed tadpoles showed transcriptional downregulation of carbohydrate and lipid catabolism, but upregulation of amino acid catabolism. It suggested a metabolic switch from carbohydrate and lipid to amino acid in these tadpoles. Accordingly, there was a trade-off between protein synthesis and energy production in respect to amino acid allocation, and it provided a metabolic explanation for why protein synthesis was downregulated and growth was suppressed in GBH-stressed tadpoles. In combination with existing literatures, we speculated that GBH might directly target the enzymes in carbohydrate and lipid catabolism, and this metabolic effect of GBH might be common to fish and amphibians. In conclusion, our study provided a systematic insight into the sublethal symptoms of GBH-stressed tadpoles, and a metabolic switch from carbohydrate and lipid to amino acid likely underlay some common toxic symptoms of GBHs on both fish and tadpoles. Image 1 • Stressed tadpoles showed warm preference, suppressed growth and tail development. • Downregulated protein synthesis underlay these sublethal effects. • GBH exposure caused metabolic switch from carbohydrate and lipid to amino acid. • GBH likely caused protein synthesis-energy production tradeoff in amino acid usage. • Impeded catabolic pathways explained the sublethal effects caused by GBH. [ABSTRACT FROM AUTHOR]

Background: Citrus flavonoids, consisting of naringin, narirutin, neohesperidine, etc., have therapeutic activities for the treatment of lipometabolic disorders. The peel of Citrus changshan-huyou (Qu Zhi Ke, QZK) is a new source of flavonoids, but attracted little attention so far.Hypothesis: QZK should possess therapeutic effects against lipometabolic disorders due to the flavonoids it contains.Study Design: In this study, we extracted and purified the flavonoids of QZK (TFCH) and established an obesity-induced non-alcoholic fatty liver disease (NAFLD) model of rats. TFCH was given orally for 8 weeks, and its anti-NAFLD effects and potential mechanism were evaluated.Methods: The flavonoid chemoprofile of TFCH was determined by using HPLC. High-fat diet was employed to induce NAFLD model in rats, and six groups were set up: negative control group, reference treatment group, model group, low-dose TFCH (25 mg/kg), intermediate-dose TFCH (50 mg/kg), and high-dose TFCH (100 mg/kg). Serum and liver levels of inflammatory cytokines and NAFLD markers were measured biochemically. The relative mRNA expressions of liver T-bet, GATA3, and TNF-α were tested by real time PCR (qPCR) analysis. The protein expression of p38 and the phosphorylation of NF-κB, ERK1/2, and p38 in liver were tested by Western blot analysis.Results: The histopathological observation showed that TFCH attenuated hepatic lesions with significantly decreased NAFLD activity scores. The biochemical data showed that TFCH significantly suppressed both systemic and intrahepatic inflammation by inhibiting IL-1β, IL-6, IL-12, TNF-α, and IFN-γ, and the qPCR analysis revealed a Th1/Th2 related anti-inflammatory mechanism of TFCH. Western blot results clarified that TFCH exerted hepatoprotective and anti-inflammatory effects by suppression of phosphorylated NF-κB and MAPKs, indicating a mechanism associated with NF-κB and MAPK signaling pathways.Conclusion: QZK is a new source of Citrus flavonoids for therapeutic use, and TFCH is a promising representative of Citrus flavonoids for anti-NAFLD therapy. [ABSTRACT FROM AUTHOR]