De novotranscriptome assembly for the lung of the ornamented pygmy frog (Microhyla fissipes)

Microhyla fissipes, belonging to Neobatrachia, is a new model organism to study developmental biology, adaptive mechanisms from aquatic to terrestrial life, environmental toxicology, and human disease. M. fissipesuse of lungs soon after hatching makes it extremely valuable for the study of lung function and development mechanisms. However, our knowledge of genes and pathways associated with lung development in M. fissipesis very limited. In this study, we conducted de novotranscriptome assembly for the lung of M. fissipesusing the Illumina HiSeq4000 platform. We obtained approximately 9.0GB clean data from the lung of the stage 28 tadpole with lung inflation (NCBI accession numbers: SRP107055). De novotranscriptome assembly identified 209,358 transcripts and 93,813 unigenes. In addition, BLASTX against NR, NT, KO, SwissProt, PFAM, GO and KOG databases were used to annotate all the 93,813 unigenes. This study provides the transcriptome and functional annotation of genes in M. fissipeslung development, which will be useful for comparative transcriptome analyses and promote research into mechanism of lung development in anuran.