80 results on '"Aoki, Takeshi"'
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2. The development of a fuel lifecycle reactivity control strategy for a generic micro high temperature reactor
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Atkinson, Seddon and Aoki, Takeshi
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This article provides an overview of the design methodology used to develop a conceptual set of reactivity control mechanism of a micro reactor based on the U-Battery. The U-Battery is based on remote deployment and therefore it is favourable to provide a long fuel lifecycle. This is achieved by implementing a high fissile loading content, which proves challenging when considering reactivity control methods. This article follows the design methodology used to overcome these issues, with an emphasis on a new concept of a moveable moderator which utilises the size of the U-Battery as a small reduction in moderation provides a significant reduction in reactivity. The latest work on this project sees the moveable moderator investigated during a depressurised loss of forced coolant accident, where a reduction of moderator volume increases the maximum fuel temperature experienced. The overall conclusion is that the maximum fuel temperature is not significantly increased (4 K) due to the central reflector region relatively lower volumetric heat capacity compared to that of whole core. However, a small temperature increase is observed immediately after the transient due to the central reflector removal because it reaches energy equilibrium with the fuel region faster.
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- 2024
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3. Feasibility of Surveillance for Mucinous Cystic Neoplasm of the Pancreas
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Hozaka, Yuto, Ohtsuka, Takao, Nakamura, Masafumi, Hijioka, Susumu, Unno, Michiaki, Shimizu, Yasuhiro, Tanabe, Minoru, Nagakawa, Yuichi, Hatano, Etsuro, Kawai, Manabu, Sano, Tsuyoshi, Koshita, Shinsuke, Katanuma, Akio, Kita, Emiri, Hanada, Keiji, Nakai, Yousuke, Aoki, Takeshi, Serikawa, Masahiro, Okamoto, Kojun, and Takeyama, Yoshifumi
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- 2023
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4. Clinical Treatment of Perioperative Disseminated Intravascular Coagulation in Patients Who Underwent Gastrointestinal and Hepato-Biliary-Pancreatic Surgery
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Tomioka, Kodai, Aoki, Takeshi, Matsuda, Kazuhiro, Enami, Yuta, Fujimori, Akira, Koizumi, Tomotake, Kusano, Tomokazu, Date, Hiromi, Yamashita, Takeshi, Ariyoshi, Tomotake, Goto, Satoru, Yamazaki, Kimiyasu, Watanabe, Makoto, Otsuka, Koji, and Murakami, Masahiko
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Background It is unclear how effective recombinant thrombomodulin (rTM) treatment is in disseminated intravascular coagulation (DIC) during the perioperative period of gastrointestinal and hepato-biliary-pancreatic surgery. The current study aimed to evaluate the therapeutic outcomes of rTM for perioperative DIC.Methods We enrolled 100 consecutive patients diagnosed with perioperative DIC after gastrointestinal surgery, and hepato-biliary-pancreatic including emergency procedures, between January 2012 and May 2021. Patients received routine rTM treatment immediately after DIC diagnosis. Then, the DIC, Sequential Organ Failure Assessment (SOFA), and Acute Physiology and Chronic Health Evaluation (APACHE) II scores were calculated and used for evaluation. The outcomes of rTM treatment and the predictors of survival were evaluated.Results The causative diseases of DIC were as follows: perforated peritonitis, n = 38; intestinal ischemia, n = 23; intra-abdominal abscess, n = 13; anastomotic leakage, n = 7; pneumonia, n = 7; cholangitis, n = 4; and others, n = 6. The 30-day mortality rate was 18.0%. There were significant differences in the platelet count (13.78 vs 10.41, P = .032) and the SOFA score (5.22 vs 9.89, P<.0001) at the start of DIC treatment between the survivor and non-survivor groups (day 0). The survivor group had a significantly lower DIC score (3.13 vs 4.93, P = .0006) and SOFA score (4.94 vs 12.14, P < .0001) and a higher platelet count (13.50 vs 4.34, P < .0001) than the non-survivor group on day 3.Conclusions Comprehensive and systemic treatment is fundamentally essential for DIC, in which rTM may play an important role in the treatment of perioperative DIC.
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- 2023
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5. Comparison of Endotoxin Activity Assay and Various Biomarkers for Severity Assessment in Colorectal Perforation Patients
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Matsuda, Kazuhiro, Aoki, Takeshi, Watanabe, Makoto, Tomioka, Kodai, Tashiro, Yoshihiko, Tomokazu, Kusano, Koizumi, Tomotake, Satoru, Goto, Yamazaki, Kimiyasu, Otsuka, Koji, and Murakami, Masahiko
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Colorectal perforation is a serious disease with high mortality requiring emergency surgery. This study aimed to evaluate the role of the endotoxin activity assay (EAA) to assess the severity in patients admitted to the intensive care unit after emergency surgeries for colorectal perforations. Patients were divided into high (EAA ≥.4) and low (EAA <.4) groups based on the EAA levels, and the correlation between the EAA values and clinical variables related to the severity was evaluated. The SOFA scores were significantly higher in the high group than those in the low group. The high EAA value persisted even after 48 hours and extended the ICU length of stay. These results suggest that EAA may be a potential biomarker to assess severity and useful as one of the instrumental in predicting the outcomes for colorectal perforation patients.
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- 2023
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6. Laparoscopic Resection of a Hepatic Epithelioid Angiomyolipoma Revealed by Indocyanine Green Fluorescence Imaging
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Mochizuki, Kiyotaka, Aoki, Takeshi, Kusano, Tomokazu, Tomioka, Kodai, Tashiro, Yoshihiko, Koizumi, Tomotake, Matsuda, Kazuhiro, Enami, Yuta, Yamochi, Toshiko, and Murakami, Masahiko
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Indocyanine green fluorescence imaging (ICG-FI)—a sensitive tool for detecting tumor localization in laparoscopic surgery—produces false positive results for benign liver tumors. This report is the first case of hepatic angiomyolipoma (HAML) treated laparoscopically with ICG-FI. We present the case of a 31-year-old woman with a liver tumor that was a 13-mm mass in the anterior superior segment. Though a benign tumor was suspected, malignant potential could not be ruled out. Therefore, minimally invasive laparoscopic resection using ICG-FI was planned. ICG, intravenously injected preoperatively, revealed the tumor’s existence. Pure laparoscopic hepatectomy with ICG-FI was performed for excisional biopsy, during which the tumor was resected with adequate surgical margins, followed by histological confirmation of HAML. In conclusion, it is suggested that laparoscopic resection with ICG-FI is an effective minimal invasive surgery for tumors that are difficult to detect, such as HAML, leading to a safe surgical margin.
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- 2023
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7. The role of bilio-pancreatic limb in nonalcoholic steatohepatitis improvement after duodenal–jejunal bypass in rats.
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Ichikawa, Hidetaka, Imoto, Hirofumi, Tanaka, Naoki, Fujishima, Fumiyoshi, Tsuchiya, Takahiro, Watanabe, Kazuhiro, Aoki, Takeshi, Kohyama, Atsushi, Morikawa, Takanori, Ohnuma, Shinobu, Naitoh, Takeshi, Kamei, Takashi, and Unno, Michiaki
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Nonalcoholic fatty liver disease, which is highly associated with obesity, includes nonalcoholic steatohepatitis. Lipopolysaccharides from the intestine would induce inflammation in the liver in nonalcoholic fatty liver disease. This study aimed to examine the role of the bilio-pancreatic limb in the effect of duodenal–jejunal bypass on nonalcoholic steatohepatitis, with respect to the gut–liver axis, using a rat model. Nonalcoholic steatohepatitis model rats were randomly assigned into 3 groups as follows: 1 sham group and 2 duodenal–jejunal bypass groups. The 2 duodenal–jejunal bypass groups were defined according to the bilio-pancreatic limb length: 30 cm (30-DJB group) and 0 cm (0-DJB group). Pathology findings and blood biochemistry, inflammatory cytokine and lipopolysaccharides receptor mRNA in the liver and common channel, and lipopolysaccharide-binding protein level in the portal vein were assessed. The reduction in plasma alanine aminotransferase and nonalcoholic fatty liver disease activity score in the 30-DJB group was not observed in the 0-DJB group, similar to the sham group. In the liver tissue, mRNA of inflammatory cytokines and lipopolysaccharide receptors, the area occupied by CD68-positive macrophages, and the number of CD3-positive T-lymphocytes on immunostaining were lower in the 30-DJB group; however, these findings were not observed in the 0-DJB group, and lipopolysaccharide-binding protein levels in the portal vein and mRNA expressions of inflammation-related genes in the common channel showed similar tendencies. The bilio-pancreatic limb plays an important role in the beneficial effect of duodenal–jejunal bypass for nonalcoholic steatohepatitis. The bilio-pancreatic limb may suppress lipopolysaccharides-related cascades in the liver by reducing intestinal inflammation. [ABSTRACT FROM AUTHOR]
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- 2021
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8. Tolvaptan for Hyponatremia Induced by Chemotherapy With Docetaxel, Cisplatin, and 5-Fluorouracil
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Hoshi, Akane, Momo, Kenji, Yamazaki, Kimiyasu, Otsuka, Koji, Aoki, Takeshi, Murakami, Masahiko, and Sasaki, Tadanori
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- 2022
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9. High-power, stable single-mode CW operation of 1550 nm wavelength InP-based photonic-crystal surface-emitting lasers
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Aoki, Takeshi, Itoh, Yuhki, Fujii, Kosuke, Yoshinaga, Hiroyuki, Fujiwara, Naoki, Ogasawara, Makoto, Sawada, Yusuke, Tanaka, Rei, Yagi, Hideki, Yanagisawa, Masaki, Yoshida, Masahiro, Inoue, Takuya, De Zoysa, Menaka, Ishizaki, Kenji, and Noda, Susumu
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1550 nm wavelength photonic-crystal surface-emitting lasers (PCSELs) are attractive for optical communication and eye-safe sensing applications. In this study, we present InP-based PCSELs featuring a double-lattice photonic-crystal structure designed for high-power single-mode operation at a wavelength of 1550 nm. These PCSELs demonstrate output powers exceeding 300 mW under continuous-wave conditions at 25 °C. Additionally, highly stable single-mode oscillation with a side-mode suppression ratio of over 60 dB is verified at temperatures from 15 °C to 60 °C. Measurement and simulation of photonic band structures reveal the impacts of the threshold gain margin and optical coupling coefficient on the single-mode stability.
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- 2024
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10. Performance of PAM-4 VCSEL for short-reach 100 Gb/s per lane applications up to 85°C
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Lei, Chun, Choquette, Kent D., Graham, Luke A., Aoki, Takeshi, Hiiro, Hiroyuki, Tanaka, Rei, Koyama, Yuji, Yoshimoto, Susumu, and Yanagisawa, Masaki
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- 2022
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11. Relationship Between Remnant Pancreatic Volume and Endocrine Function After Pancreaticoduodenectomy
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Yamazaki, Tatsuya, Aoki, Takeshi, Tashiro, Yoshihiko, Koizumi, Tomotake, Kusano, Tomokazu, Matsuda, Kazuhiro, Fujimori, Akira, Yamada, Kosuke, Nogaki, Koji, Hakozaki, Tomoki, Wada, Yusuke, Shibata, Hideki, Tomioka, Kodai, Enami, Yuta, and Murakami, Masahiko
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Background Decreased pancreatic volume (PV) is a predictive factor for diabetes mellitus (DM) after surgery. There are few reports on PV and endocrine function pre- and post-surgery. We investigated the correlation between PV and insulin secretion.Methods Seventeen patients underwent pancreaticoduodenectomy (PD) Pre- and post-surgery PV and C-peptide index (CPI) measurements were performed. Additionally, the correlation between PV and CPI was analyzed.Results The mean preoperative PV (PPV) was 55.1 ± 31.6 mL, postoperative remnant PV (RPV) was 25.3±17.3 mL, and PV reduction was 53%. The mean preoperative C-peptide immunoreactivity (CPR) was 1.39 ± .51 and postoperative CPR was .85±.51. The mean preoperative CPI was 1.29±.72 and postoperative CPI was .73 ± .48. Significant correlations were observed between RPV and post CPR (ρ = .507, P = .03) and post CPI (ρ = .619, P = .008).Discussion There was a significant correlation between RPV and CPI after PD. A smaller RPV resulted in lower insulin secretion ability, increasing the potential risk of new-onset DM after PD.
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- 2022
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12. Experience of the pancreas duodenectomy for so-called carcinosarcoma of the common bile duct:a case report and review of literature.
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Sasamoto, Suguru, Aoki, Takeshi, Tashiro, Yoshihiko, Matsuda, Kazuhiro, Koizumi, Tomotake, Kusano, Tomokazu, Wada, Yusuke, Shibata, Hideki, Tomioka, Kodai, Yamashita, Takeshi, Date, Hiromi, Ariyoshi, Tomotake, Goto, Satoru, Yamazaki, Kimiyasu, Fujimori, Akira, Watanabe, Makoto, Enami, Yuta, Otsuka, Koji, Norose, Tomoko, and Ohike, Nobuyuki
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- 2021
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13. Safety of Combined Division vs Separate Division of the Splenic Vein in Patients Undergoing Distal Pancreatectomy: A Noninferiority Randomized Clinical Trial
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Yamada, Suguru, Fujii, Tsutomu, Sonohara, Fuminori, Kawai, Manabu, Shibuya, Kazuto, Matsumoto, Ippei, Fukuzawa, Kengo, Baba, Hideo, Aoki, Takeshi, Unno, Michiaki, Satoi, Sohei, Kishi, Yoji, Hatano, Etsuro, Uemura, Kenichiro, Horiguchi, Akihiko, Sho, Masayuki, Takeda, Yutaka, Shimokawa, Toshio, Kodera, Yasuhiro, and Yamaue, Hiroki
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IMPORTANCE: In distal pancreatectomy (DP), the splenic vein is isolated from the pancreatic parenchyma prior to being ligated and divided to prevent intra-abdominal hemorrhage from the splenic vein stump with pancreatic fistula (PF). Conversely, dissecting the splenic vein with the pancreatic parenchyma is easy and time-saving. OBJECTIVE: To establish the safety of combined division of the splenic vein compared with separate division of the splenic vein. DESIGN, SETTING, AND PARTICIPANTS: This study was designed as a multicenter prospective randomized phase 3 trial. All results were analyzed using the modified intent-to-treat set. Patients undergoing DP for pancreatic body and tail tumors were eligible for inclusion. Patients were randomly assigned between August 10, 2016, and July 30, 2019. INTERVENTIONS: Patients were centrally randomized (1:1) to either separate division of the splenic vein or combined division of the splenic vein. MAIN OUTCOMES AND MEASURES: The primary end point was the incidence of grade B/C PF, and the incidence of intra-abdominal hemorrhage was included as one of the secondary end points. RESULTS: A total of 318 patients were randomly assigned, and 2 patients were excluded as ineligible. Of the 316 remaining patients, 150 (50.3%) were male. The modified intent-to-treat population constituted 159 patients (50.3%) in the separate division group and 157 patients (49.7%) in the combined division group. In the modified intent-to-treat set, the proportion of grade B/C PF in the separate division group was 27.1% (42 of 155) vs 28.6% (44 of 154) in the combined division group (adjusted odds ratio, 1.108; 95% CI, 0.847-1.225; P = .047), demonstrating noninferiority of the combined division of the splenic vein against separate division. The incidence of postoperative intra-abdominal hemorrhage in the 2 groups was identical at 1.3%. CONCLUSIONS AND RELEVANCE: This study demonstrated noninferiority of the combined division of the splenic vein compared with separate division of the splenic vein regarding safety. Thus, isolating the splenic vein from the pancreatic parenchyma is deemed unnecessary. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT02871804
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- 2021
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14. Safe and Accurate Laparoscopic Total Gastrectomy for Remnant Gastric Cancer With Severe Adhesions Using Indocyanine Green Fluorescence Imaging: Guidance in Detection of the Accurate Dissection Layer to Avoid Organ Injury
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Yamazaki, Kimiyasu, Mochizuki, Kiyotaka, Date, Hiromi, Watanabe, Ryohei, and Aoki, Takeshi
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Laparoscopic total gastrectomy (LTG) for remnant gastric cancer (RGC) requires advanced techniques due to severe postoperative adhesions and anatomic changes. We performed LTG in 2 patients with RGC using intraoperative indocyanine green (ICG) fluorescence imaging. Both cases previously underwent distal gastrectomy with Billroth-I reconstruction for gastric cancer and were subsequently diagnosed with early-stage gastric cancer of the remnant stomach. Indocyanine green (2.5 mg/body) was administered intravenously during surgery. The liver and common bile duct were clearly visualized during surgery using near-infrared fluorescence laparoscopy, and the adhesions between the hepatobiliary organs and remnant stomach were safely dissected. Laparoscopic total gastrectomy was successfully performed without complications, and the postoperative course was uneventful in both cases. Intraoperative real-time ICG fluorescence imaging allows clear visualization of the liver and common bile duct and can be useful in LTG for RGC with severe adhesions.
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- 2024
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15. The Prognostic Impact of Eosinophils and the Eosinophil-to-Lymphocyte Ratio on Survival Outcomes in Stage II Resectable Pancreatic Cancer
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Ohkuma, Ryotaro, Kubota, Yutaro, Horiike, Atsushi, Ishiguro, Tomoyuki, Hirasawa, Yuya, Ariizumi, Hirotsugu, Watanabe, Makoto, Onoue, Rie, Ando, Kiyohiro, Tsurutani, Junji, Yoshimura, Kiyoshi, Aoki, Takeshi, Murakami, Masahiko, Kobayashi, Shinichi, Tsunoda, Takuya, and Wada, Satoshi
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Supplemental digital content is available in the text.
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- 2021
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16. Uric acid distribution volume calculated by kinetic modeling and extracellular volume predicted by bioimpedance method
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Shinzato, Toru, Nakai, Shigeru, Ito, Takahito, Shibata, Kazuhiko, Matsuoka, Teppei, Kato, Shinya, Aoki, Takeshi, Masakane, Ikuto, Hayashi, Hiroki, Tsuboi, Naotake, Hasegawa, Midori, Inaguma, Daijo, Yuzawa, Yukio, and Ookawara, Susumu
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Background: Several reports indicate that extracellular volume predicted by bioimpedance analysis method is associated with hydration status of hemodialysis patients.Theory: Fundamentally, uric acid does not cross cell membranes by simple diffusion, either by facilitated diffusion or by active transport. In addition, uric acid cannot move through cell membranes in most tissues other than those involved in uric acid excretion. These facts support the interpretation that uric acid distribution volume would therefore correlate with extracellular volume.Methods: We examined correlation between uric acid distribution volume calculated by uric acid mass-balance modeling from regular blood test results and extracellular volume predicted by bioimpedance analysis predicted by BCM (Fresenius Medical Care) in 53 patients.Results: There was a significant correlation between uric acid distribution volume (x) and extracellular volume predicted by bioimpedance analysis (y): y= 0.69x+ 3.39, r2= 0.61, p< 0.0001. Bland–Altman analysis showed systematic error for uric acid distribution volume versus extracellular volume predicted by bioimpedance analysis (mean difference between uric acid distribution volume and extracellular volume predicted by bioimpedance analysis was 0.94 L, 95% confidence interval of difference was −3.29 to 5.17 L).Conclusion: Uric acid distribution volume calculated by uric acid mass-balance modeling from regular blood test results may be an alternative marker of extracellular volume predicted by bioimpedance analysis.
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- 2020
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17. Minimally invasive esophagectomy and radical lymph node dissection without recurrent laryngeal nerve paralysis
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Otsuka, Koji, Murakami, Masahiko, Goto, Satoru, Ariyoshi, Tomotake, Yamashita, Takeshi, Saito, Akira, Kohmoto, Masahiro, Kato, Rei, Lefor, Alan Kawarai, and Aoki, Takeshi
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Background: We introduce a novel operative technique to dissect lymph nodes adjacent to the recurrent laryngeal nerve, referred to as the “native tissue preservation” technique. Using this technique, there was no damage to the recurrent laryngeal nerve, which is maintained in its anatomical position. Methods: From September 2016 to December 2018, minimally invasive esophagectomy was performed in the left lateral decubitus position in 87 patients with esophageal cancer. The native tissue preservation technique for lymphadenectomy around the recurrent laryngeal nerve was used, and all patients were evaluated for recurrent laryngeal nerve paralysis. Results: Minimally invasive esophagectomy was completed in all patients without conversion to thoracotomy. Although an extended lymphadenectomy was performed in all patients, there were no grade II or higher complications (Clavien–Dindo classification) and no incidence of recurrent laryngeal nerve paralysis. Conclusion: The native tissue preservation technique may reduce the incidence of recurrent laryngeal nerve paralysis after minimally invasive esophagectomy with radical lymph node dissection.
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- 2020
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18. 1×4 VCSEL arrays with uniform spectral and noise properties by using rotationally asymmetric oxide aperture for 400 Gbit/s applications
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Graham, Luke A., Lei, Chun, Inoue, Daisuke, Kubota, Ryosuke, Aoki, Takeshi, Ishizuka, Takashi, Yanagisawa, Masaki, and Shoji, Hajime
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- 2020
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19. Prediction of the Probability of Malignancy in Mucinous Cystic Neoplasm of the Pancreas With Ovarian-Type Stroma
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Ohtsuka, Takao, Nakamura, Masafumi, Hijioka, Susumu, Shimizu, Yasuhiro, Unno, Michiaki, Tanabe, Minoru, Nagakawa, Yuichi, Takaori, Kyoichi, Hirono, Seiko, Gotohda, Naoto, Kimura, Wataru, Ito, Kei, Katanuma, Akio, Sano, Tsuyoshi, Urata, Takahiro, Kita, Emiri, Hanada, Keiji, Tada, Minoru, Aoki, Takeshi, Serikawa, Masahiro, Okamoto, Kojun, Isayama, Hiroyuki, Gotoh, Yoshitaka, Ishigami, Kousei, Yamaguchi, Hiroshi, Yamao, Kenji, Sugiyama, Masanori, and Okazaki, Kazuichi
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- 2020
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20. P‐54: An Oxide‐Semiconductor‐FET‐Based Dynamic Logic Circuit for Wearable Systems
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Hamada, Toshiki, Takeuchi, Toshihiko, Aoki, Takeshi, Kozuma, Munehiro, Ikeda, Takayuki, Ikeda, Makoto, and Yamazaki, Shunpei
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We propose a wearable system using oxide semiconductor (OS) transistors, which enables reduced static power of a dynamic logic device utilizing OS transistors and resulting longer continuous use of a wearable device. We fabricated a wearable‐watch‐oriented device. The results indicated that the static power could be reduced by 51%.
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- 2019
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21. Efficacy of ‘drive and retrieve’ as a cooperative method for prompt endovascular treatment for acute ischemic stroke
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Osanai, Toshiya, Ito, Yasuhiro, Ushikoshi, Satoshi, Aoki, Takeshi, Kawabori, Masahito, Fujiwara, Kensuke, Ogasawara, Katsuhiko, Tokairin, Kikutaro, Maruichi, Katsuhiko, Nakayama, Naoki, Kazumata, Ken, Ono, Kota, and Houkin, Kiyohiro
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BackgroundOutcomes of endovascular treatment for acute ischemic stroke depend on the time interval from onset to reperfusion. Although the centralized ‘mothership’ method is considered preferable, the required transportation time increases the risk that a patient with a stroke may not receive intravenous or endovascular therapy. In contrast, ‘drive and retrieve’ describes a system wherein doctors from comprehensive stroke centers travel to primary stroke centers and provide endovascular treatment for acute ischemic stroke.ObjectiveTo describe the drive and retrieve system and verify the effects of this new collaboration on outcomes in patients with acute ischemic stroke among facilities.MethodsThis non-randomized, single-arm study retrospectively analyzed patients who met the inclusion criteria for endovascular treatment provided through a drive and retrieve system. Among the 122 patients treated by this system, we analyzed the time of onset to recanalization as the primary outcome. We also analyzed the efficacy of the drive and retrieve system using geographic information system analysis.ResultsThe median time from onset to recanalization was 229 min (IQR 170–307 min, 95% CI 201 to 252 min). The upper limit of the 95% CI for the time from onset to recanalization was shorter than the median times reported in two previous trials. Geographic information system analysis revealed an upward trend in the population coverage rate in each secondary medical area after the drive and retrieve method was introduced.ConclusionThe drive and retrieve method may be an effective form of cooperation between facilities located within 1 hour of a comprehensive stroke center.
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- 2019
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22. Endoscopic clipping of spontaneous esophageal rupture: Case reports of three patients.
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Otsuka, Koji, Murakami, Masahiko, Ariyoshi, Tomotake, Yamashita, Takeshi, Goto, Satoru, Watanabe, Makoto, and Aoki, Takeshi
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Introduction Spontaneous esophageal rupture is a life-threatening condition which is difficult to diagnose early, and is usually treated surgically. Prolonged hospitalization is common. Non-operative treatment of esophageal rupture localized to the mediastinum has been reported. We report three patients with spontaneous esophageal rupture successfully managed with endoscopic clipping. Case presentations Two patients had ruptures localized to the mediastinum, and were discharged within one week of undergoing closure. The third patient presented with Boerhaave’s syndrome with a leak into the pleural space and needed prolonged hospitalization (34 days), but she did not need surgery and began oral intake two days after endoscopic clipping. The patient had an uneventful recovery. [ABSTRACT FROM AUTHOR]
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- 2017
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23. Simultaneous tumor identification, cholangiography, and securing surgical margin for recurrence of hepatocellular carcinoma using the Medical Imaging Projection System
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Tashiro, Yoshihiko, Aoki, Takeshi, Kusano, Tomokazu, and Matsuda, Kazuhiro
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Indocyanine green (ICG) fluorescence-guided surgery is a real-time navigation technology for tumor detection, securing surgical margins, segmentation mapping, and cholangiography in liver surgery [1]. According to recent reports, the Medical Imaging Projection System (MIPS) may be a useful new real-time navigation technology for open anatomical liver resection [2]. However, the efficacy of MIPS for tumor identification, cholangiography, and securing surgical margins is uncertain. In this report, we introduce MIPS-assisted liver resection for real-time navigation during simultaneous tumor identification, cholangiography, and securing surgical margins.
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- 2023
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24. Risk for Major Bleeding in Patients Receiving Ticagrelor Compared With Aspirin After Transient Ischemic Attack or Acute Ischemic Stroke in the SOCRATES Study (Acute Stroke or Transient Ischemic Attack Treated With Aspirin or Ticagrelor and Patient Outcomes)
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Easton, J. Donald, Aunes, Maria, Albers, Gregory W., Amarenco, Pierre, Bokelund-Singh, Sara, Denison, Hans, Evans, Scott R., Held, Peter, Jahreskog, Marianne, Jonasson, Jenny, Minematsu, Kazuo, Molina, Carlos A., Wang, Yongjun, Wong, K.S. Lawrence, Johnston, S. Claiborne, Ameriso, Sebastiá F., Donnan, Geoffrey, Lemmens, Robin, Massaro, Ayrton, Titianova, Ekaterina, Hill, Michael D., Lavados, Pablo, Skoloudik, David, Röther, Joachim, Norbert, Szegedi, Agnelli, Giancarlo, Bornstein, Natan, Tanahashi, Norio, Góngora, Angel Arauz, Pretell, Edwin, San Jose, Maria Cristina Z., Czlonkowska, Anna, Bajenaru, Ovidiu, Stakhovskaya, Ludmila, Brozman, Miroslav, Kim, Jong-Sung, Wahlgren, Nils, Michel, Patrik, Lee, Tsong Hai, Suwanwela, Nijasri Charnnarong, Kutluk, Kursad, Moskovko, Sergii, Kasner, Scott, Laskowitz, Daniel, Clark, Wayne, Nguyen, Huy Thang, Ameriso, Sebastian, Lepera, Sandra, Romano, Marina, Paulon, David, Ioli, Pablo, Zurru, Cristina, Bruera, Guadalupe, Jure, Lorena, Klein, Francisco, Povedano, Guillermo, Levi, Christopher, Phan, Thanh, Markus, Romesh, Anderson, Craig, Sabet, Arman, Davis, Stephen, Lee, Andrew, Kleinig, Timothy, Wong, Andrew, Krause, Martin, Jannes, Jim, Wijeratne, Tissa, Lemmens, Robin, Hemelsoet, Dimitri, Peeters, André, Tack, Philippe, Vanacker, Peter, Laloux, Patrice, Van Landegem, William, Vanhooren, Geert, Desfontaines, Philippe, Van Orshoven, Marc, Oliveira, Fabio, Friedrich, Mauricio, Brondani, Rosane, Gagliardi, Rubens, Fabio, Soraia, Dracoulakis, Marianna, Bazan, Rodrigo, Marrone, Luiz, Pontes Neto, Octavio, Silva, Gisele, Kowacs, Pedro, Titianova, Ekaterina, Stamenova, Paraskeva, Daskalov, Marin, Staikov, Ivan, Baldaranov, Dimo, Maslarov, Dimitar, Lilovski, Hristo, Petkov, Plamen, Petrova, Neli, Mavrov, Radoslav, Markova, Veska, Petrova, Valeria, Beleva, Tanya, Kralev, Borislav, Sotirov, Nikolay, Lekova, Veska, Hristov, Dimcho, Ermenkova, Vera, Mateev, Lyudmil, Mitkova, Rumeliya, Haralanov, Liybomir, Ikonomov, Rosen, Mihailova, Margarita, Georgiev, Ivan, Shuaib, Ashfaq, Hachinski, Vladimir, Boulanger, Jean-Martin, Mann, Sharan, Hassan, Ayman, Mackey, Ariane, Menon, Bijoy, Minuk, Jeffrey, Siddiqui, Muzaffar, Eustace, Marsha, Vieira, Lucia, Selchen, Daniel, Beaudry, Michel, Stotts, Grant, Lavados, Pablo, Castro, Angel, Gasic, Kristo, Rivas, Rodrigo, Sanchez, Pablo, Roldan, Andres, Grossmann, Ingrid, Figueroa, Christian, Li, Jimei, Xu, Xiaolin, Chen, Huisheng, Li, Xiaohong, Yang, Yi, Zhang, Chunsheng, Wang, Baojun, Li, Guanglai, Wang, Dong, Lin, Hong, Tang, Yamei, Xu, Anding, Wang, Yanjiang, Hong, Wenke, Song, Zhi, Zhang, Xu, Jin, Xiaoping, Xu, Yun, Yan, Fuling, Zheng, Weihong, Wang, Xiaoping, Dong, Qiang, Zhao, Zhongxin, Zhang, Baorong, Zhong, Wangtao, Wen, Guoqiang, Xu, Jun, Li, Guozhong, Dong, Xueshuang, Tian, Xiangyang, Zhang, Zhaohui, Xu, En, Liu, Kaixiang, Chen, Jun, Skoda, Ondrej, Skoloudik, David, Ehler, Edvard, Vaclavik, Daniel, Sanak, Daniel, Klimosova, Sylva, Vitkova, Eva, Fiksa, Jan, Mikulik, Robert, Neumann, Jiri, Plny, Richard, Leys, Didier, Sibon, Igor, Mas, Jean-Louis, Alamowitch, Sonia, Pico, Fernando, Hosseini, Hassan, Mahagne, Marie-Hélène, Touze, Emmanuel, Vadot, Wilfried, Vannier, Stéphane, Nighoghossian, Norbert, Samson, Yves, Garnier, Pierre, Ellie, Emmanuel, Guillon, Benoît, Timsit, Serge, Giroud, Maurice, Philippeau, Frédéric, Bagan-Triquenot, Aude, Wolff, Valérie, Raposo, Nicolas, Obadia, Michel, Debiais, Severine, Grimaud, Jérôme, Illouz, Stéphane, Smadja, Didier, Urbanczyk, Cédric, Röther, Joachim, Berrouschot, Jörg, Weimar, Christian, Gahn, Georg, Soda, Hassan, Klimpe, Sven, Nabavi, Darius, Glahn, Jörg, Köhrmann, Martin, Krause, Lars, Terborg, Christoph, Urban, Peter, Steiner, Thorsten, Ferbert, Andreas, Dziewas, Rainer, Seidel, Günter, Thomalla, Götz, Li, Richard, Fong, Wing Chi, Cheung, Raymond, Szegedi, Norbert, Pozsegovits, Krisztián, Valikovics, Attila, Pánczél, Gyula, Rózsa, Csilla, Németh, László, Diószeghy, Péter, Óváry, Csaba, Csányi, Attila, Kerényi, Levente, Nagy, Valéria, Komoly, Sámuel, Bereczki, Dániel, Molnár, Sándor, Kondákor, István, Tanne, David, Raphaeli, Guy, Telman, Gregory, Bornstein, Natan, Leker, Ronen, Lampl, Yair, Agnelli, Giancarlo, Corea, Francesco, Ricci, Stefano, Guidetti, Donata, Malferrari, Giovanni, Marcheselli, Simona, Micieli, Giuseppe, Zini, Andrea, Di Lazzaro, Vincenzo, Gandolfo, Carlo, Salmaggi, Andrea, Tassi, Rossana, Rasura, Maurizia, Orlandi, Giovanni, Comi, Giancarlo, Ricci, Stefano, Mancuso, Michelangelo, Delodovici, Marialuisa, Bovi, Paolo, Consoli, Domenico, Utsugisawa, Kimiaki, Fujita, Tsuneo, Kurihara, Hideyuki, Maruki, Chikashi, Hayashi, Takeshi, Ogiichi, Tsuneaki, Kumagai, Morio, Takenaka, Katsunobu, Toyoda, Kazunori, Takamatsu, Kazuhiro, Ogami, Ryo, Kin, Shigenari, Aoki, Takeshi, Takizawa, Katsumi, Omori, Shigehiro, Umezawa, Takehiko, Toba, Yasuyuki, Nonoyama, Yutaka, Nakagawa, Hidemitsu, Naka, Takashi, Morimoto, Masanori, Matsumoto, Shuichi, Hitotsumatsu, Tsutomu, Shingaki, Tatsuya, Okuda, Satoshi, Ota, Mamoru, Sakai, Nobuyuki, Yamada, Takeshi, Niwa, Jun, Fujita, Hitoshi, Moriki, Akihito, Yoshino, Kimihiro, Fukushima, Yoshihisa, Mori, Takahisa, Sato, Atsushi, Kusano, Yoshikazu, Kubo, Michiya, Yamazaki, Masashi, Ooasa, Takao, Nishizaki, Takafumi, Kitagawa, Naoki, Yasaka, Masahiro, Manabe, Yasuhiro, Yoshioka, Akira, Ishihara, Masayuki, Kagawa, Takato, Ichihashi, Toshikazu, Matsuoka, Hideki, Ito, Yasuhiro, Yamasaki, Masahiro, Takaba, Hitonori, Saito, Hisatoshi, Sato, Masahiro, Fukuda, Kazumasa, Endo, Sumio, Kidooka, Minoru, Umemura, Toshitaka, Kikkawa, Yuriko, Toru, Shuta, Yamada, Kentaro, Sakai, Hideki, Asari, Jun, Ezura, Masayuki, Nitta, Hisashi, Nagano, Keiko, Ochiai, Jun, Sakai, Keiichi, Kobayashi, Yasutaka, Yoshii, Yasuhiro, Miake, Hirotomo, Takita, Tomohiro, Taniguchi, Hidekazu, Kuroki, Kazuhiko, Mizota, Takamitsu, Yamamoto, Kenichi, Nakane, Hiroshi, Iwanaga, Takeshi, Chiba, Kei, Yoshimoto, Tetsuyuki, Torii, Tsuyoshi, Kitagawa, Takeo, Takashima, Hiroshi, Shirasaki, Naoki, Dehara, Makoto, Wada, Naomichi, Hamada, Kensuke, Kato, Noriyuki, Go, Yoshinori, Izumi, Ichiro, Ninomiya, Hirotomo, Kumai, Junichiro, Nakajima, Yoshikazu, Kaku, Yasuhiko, Isayama, Yukihiro, Kawanishi, Masahiro, Noda, Shinya, Yamamoto, Kazuhide, Hazama, Takanori, Takahashi, Hiroshi, Tanaka, Yohei, Hata, Takashi, Kazekawa, Kiyoshi, Furui, Eisuke, Hondo, Hideki, Sato, Nobuyuki, Kusunoki, Katsusuke, Nanri, Kazunori, Abe, Satoshi, Sasaoka, Noboru, Kuroyanagi, Takayuki, Suzuki, Hisahiko, Fukuyama, Kouzou, Nakahara, Kimihiro, Gongora, Fernando, Góngora, Angel Arauz, Cantú Brito, Carlos, Villarreal Careaga, Jorge, Vazquez Alfaro, Rosalia, Aguayo Leytte, Geronimo, Berrospi, Percy, Chavez, Carlos, Pretell, Edwin, Rodriguez, Liliana, Custodio, Nilton, Castañeda, Cesar, Perez, Julio, San Jose, Maria Cristina, Baroque, Alejandro, Collantes, Epifania, Aquino, Abdias, Díaz, Alejandro, Roxas, Artemio, Lokin, Johnny, Advincula, Joel, Calderon, Emerito, Navarro, Jose, Hiyadan, John, Surdilla, Arturo, Czlonkowska, Anna, Ryglewicz, Danuta, Krychowiak, Grzegorz, Fryze, Waldemar, Sobolewski, Piotr, Nowak, Ryszard, Fiszer, Urszula, Papierowska, Beata, Zielińska-Turek, Justyna, Lasek-Bal, Anetta, Kołodziejska, Ewa, Kamińska, Anna, Adamkiewicz, Bożena, Tutaj, Andrzej, Szkopek, Dorota, Musiatowicz, Krzysztof, Bąk, Zbigniew, Brzozowski, Sławomir, Brola, Waldemar, Ferens, Antoni, Zalisz, Marek, Rejdak, Konrad, Rudzińska, Monika, Bajenaru, Ovidiu, Panea, Cristina, Simu, Mihaela, Balasa, Rodica, Cuciureanu, Iulian, Popescu, Bogdan, Sabau, Monica, Roman-Filip, Corina, Pimenov, Leonid, Gekht, Alla, Milto, Anna, Shchukin, Ivan, Parfenov, Vladimir, Stakhovskaya, Liudmila, Arkhipov, Mikhail, Sokolova, Nadezhda, Bogdanov, Enver, Esin, Radiy, Khasanova, Dina, Golikov, Konstantin, Melnikova, Elena, Zaslavskiy, Leonid, Voznyuk, Igor, Nazarov, Alexander, Akhmadeeva, Leila, Iakupova, Aida, Shamalov, Nikolay, Belskaya, Galina, Chuprina, Svetlana, Denisova, Olga, Drozdova, Ekaterina, Karakulova, Yuliya, Sholomov, Ilya, Spirin, Nikolay, Vostrikova, Elena, Mordvintseva, Elena, Grigoryeva, Vera, Zateyshchikov, Dmitry, Gorbachev, Vladimir, Chefranova, Zhanna, Dudarev, Mikhail, Nilk, Rostislav, Rozhdestvenskiy, Alexey, Gurcik, Ladislav, Dvorak, Miloslav, Krastev, Georgi, Kurca, Egon, Vyletelka, Juraj, Brozman, Miroslav, Kim, Jong Sung, Bae, Hee-Joon, Kim, Yong-Won, Kim, Joon-Tae, Cha, Jae-Kwan, Nam, Hyo Suk, Chang, Dae-Il, Lee, Yong-Seok, Oh, Kyungmi, Yu, Sung-Wook, Sohn, Sung-Il, Lee, Jun, Cho, Han Jin, Kim, Eung-Gyu, Rha, Joung-Ho, Kim, Seo Hyun, Molina Cateriano, Carlos, Serena Leal, Joaquín, Vivancos Mora, José, Rodríguez Yañez, Manuel, Roquer González, Jaume, Purroy García, Francisco, Gomis Cortina, Meritxell, Masjuan Vallejo, Jaime, Arenillas Lara, Juan, Segura Martín, Tomás, Herrero, José Antonio Egido, Tembl Ferrairó, Jose Ignacio, Gállego Culleré, Jaime, Moniche Álvarez, Francisco, Steinberg, Anna, Callander, Margarita, Laska, Ann Charlotte, Bokemark, Lena, Mooe, Thomas, Käll, Tor-Björn, Welin, Lennart, Sjöblom, Lars, Hambraeus, Joakim, Teichert, Jörg, Wannberg, Hans, Sanner, Johan, Ramströmer, Bo, Ziedén, Bo, Hau, Stefan Olsson, Gustafsson, Claes, Michel, Patrik, Kahles, Timo, Lyrer, Philippe, Arnold, Marcel, Liesch, Martin, Medlin, Friedrich, Cereda, Carlo, Kägi, Georg, Luft, Andreas, Carrera, Emmanuel, Lee, Tsong-Hai, Po, Helen L., Chern, Chang-Ming, Lien, Li-Ming, Chan, Lung, Liu, Chung-Hsiang, Wu, Shey-Lin, Lee, Jiann-Der, Chen, Chih-Hung, Lin, Huey-Juan, Lin, Ruey-Tay, Chen, Wei-Hsi, Sun, Yu, Suwanwela, Nijasri Charnnarong, Tantirittisak, Tasanee, Muengtaweepongsa, Sombat, Nilanont, Yongchai, Tiamkao, Somsak, Udommongkol, Chesda, Watcharasaksilp, Kanokwan, Jantararotai, Witoon, Kutluk, Kursad, Sirin, Hadiye, Ince, Birsen, Asil, Talip, Arsava, Murat, Incesu, Tulay Kurt, Tireli, Hulya, Kucukoglu, Hayriye, Ak, Fikri, Unal, Ali, Ozturk, Serefnur, Uzuner, Nevzat, Chmyr, Galyna, Lebedynets, Volodymyr, Nikonov, Vadym, Shulga, Lyudmyla, Smolanka, Volodymyr, Moskovko, Sergii, Khavunka, Marta, Yavorska, Valentyna, Tomakh, Nataliya, Kozyolkin, Olexandr, Litovaltseva, Galyna, Lansberg, Maarten, Bernstein, Richard, Brown, David, Clark, Wayne, Dissin, Jonathan, Graffagnino, Carmelo, Harris, Jonathan, Hicks, William, Kasner, Scott, Katzan, Irene, Kramer, Jeffrey, Willey, Joshua, Silliman, Scott, Starkman, Sidney, Thaler, David, Tremwel, Margaret, Concha, Mauricio, Rajamani, Kumar, Dandapani, Bhuvaneswari, Silver, Brian, Deal, Nathan, Chang, Ira, Hassan, Ameer, Rudolph, Steven, Fischer, Kenneth, Kirshner, Howard, Logan, William, Mallenbaum, Sidney, Hefzy, Hebah, Latorre, Julius, Levine, Steven, Ciabarra, Anthony, Dafer, Rima, Anyanwu, Benjamin, Cherian, Laurel, Panezai, Spozhmy, Khanna, Anna, Dodds, Jodi, Torbey, Michel, Gebel, James, Woo, Henry, Chiu, David, Androulakis, Xiao, Burgin, William, Pineda, Maria, Yilmaz, Engin, Altafullah, Irfan, Boutwell, Christine, Cruz-Flores, Salvador, Sapkota, Biggya, Fayad, Pierre, Jacoby, Michael, Rafiq, Shahid, Salgado, Efrain, Lafranchise, Eugene, Felton, Warren, Madhavan, Ramesh, Zaidat, Osama, Pieper, Connie, Riviello, Ralph, Burnett, Aaron, Fischer, Michelle, Gentile, Nina, Calder, Christopher, Dietrich, Dennis, Cross, Jonathan, Blankenship, Larry, Dafer, Rima, Montoya, Liliana, Grogan, Wendell, Young, Mark, Khan, Farrukh, Campbell, Duane, Daboul, Nizar, Espinoza, Andrey, Cullis, Paul, Concepcion, Gilberto, Wulff, John, Afzal, Haider, Jaffrani, Naseem, Reiter, William, Arshad, Tamjeed, Lukovits, Timothy, Welker, James, Chang, Fen Lei, Badruddin, Aamir, Babikian, Viken, Menon, Ravi, Sander, James, Springer, Mellanie, Nanda, Ashish, Mas, Luis, Rajan, Raj, Silverman, Bruce, Hefzy, Hebah, Huang, David, Carpenter, David, Clark, Joni, Ching, Marilou, Santhakumar, Sunitha, Gould, Jeffrey, Bansal, Vibhav, Vidal, Gabriel, Mikesell, Timothy, Brick, John, French, William, Shah, Qaisar, Holmstedt, Christine, Ishag-Osman, Nadir, Kostis, John, Shehadeh, Abbas, Sethi, Pramodkumak, Imam, Asher, Mccomas, Carl, Tran, Duc, Gebreyohanns, Mehari, Wiseman, Brian, Malik, Maheen, Schwarcz, Aron, Altschul, Dorothea, Castaldo, John, Alshekhlee, Amer, Gancher, Stephen, Krish, Nagesh, Nguyen-Huynh, Mai, Tremwell, Margaret, Sharma, Jitendra, Lee, Lance, Neil, William, Siddiqui, Fazeel, Malek, Ali, Romero, Charles, Huy, Thang Nguyen, Hoang, Hoa, Nguyen, Thang, Nguyen, Anh, and Nguyen, Hung
- Abstract
Supplemental Digital Content is available in the text.
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- 2017
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25. 16-1: Low-Power Oxide-Semiconductor Display System
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Kurokawa, Yoshiyuki, Kunitake, Hitoshi, Harada, Shintaro, Akasawa, Fumika, Okamoto, Yuki, Nakagawa, Takashi, Aoki, Takeshi, Yoneda, Seiichi, Inoue, Hiroki, Kozuma, Munehiro, Ikeda, Takayuki, Yao, Hai Biao, Shuai, Chi Chang, Lin, Hung Chan, Wu, Shao Hui, Ikeda, Makoto, and Yamazaki, Shunpei
- Abstract
An oxide semiconductor (OS) display system built with normally-off devices, nonvolatile OS LSI and an OS display, is proposed. The power consumption of the system is reduced by the nonvolatile OS LSI performing power gating without vanishing setting data when the OS display is in an idling stop (IDS) mode.
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- 2017
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26. P-46: An Oxide-Semiconductor (OS) Technology-Based Display Controller Suitable for an OS Display Comprising a Non-Volatile Scan Register for Display-Parameter Setting
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Harada, Shintaro, Kozuma, Munehiro, Inoue, Hiroki, Aoki, Takeshi, Okamoto, Yuki, Kurokawa, Yoshiyuki, Ikeda, Takayuki, Jia, Xiao Yu, Kao, Tai-Chi, Lin, Hung-Chan, Wu, Shao Hui, Ikeda, Makoto, and Yamazaki, Shunpei
- Abstract
A display controller used in an oxide-semiconductor (OS) display was fabricated based on OS technology. Setting parameters were stored in non-volatile registers comprising OS FETs; thus, power shutdown and restart of the power supply along with “idling stop” driving are possible. The power consumption was reduced by 75%. Optimal setting parameters can also be obtained with AI.
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- 2017
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27. Low-Power Display System Enabled by Combining Oxide-Semiconductor and Neural-Network Technologies
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Kunitake, Hitoshi, Harada, Shintaro, Akasawa, Fumika, Okamoto, Yuki, Nakagawa, Takashi, Aoki, Takeshi, Yoneda, Seiichi, Inoue, Hiroki, Kozuma, Munehiro, Ikeda, Takayuki, Kurokawa, Yoshiyuki, and Yamazaki, Shunpei
- Abstract
Oxide semiconductor field-effect transistors (OSFETs) are characterized by their extremely low off-state current. By fabricating both a display and peripheral large-scale integrated circuits with OSFETs, the components can be driven in a normally off manner, thus enabling a display system with low power consumption. We explore herein the feasibility of a more energy-efficient display system, which is achieved by predicting changes in image data and thus more efficiently controlling the power-gate timing. This is done by using an artificial neural network.
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- 2017
28. Localized reversible high signal intensities on diffusion-weighted MRI in hypoglycemia: A study of 70 cases
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Katoh, Masahito, Yoshino, Masami, Aoki, Takeshi, Abumiya, Takeo, Imamura, Hiroyuki, and Aida, Toshimitsu
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- 2016
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29. Development of Pneumatically Controlled Expandable Arm for Search in the Environment with Tight Access.
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Yuta, Shin'ichi, Asama, Hajima, Prassler, Erwin, Tsubouchi, Takashi, Thrun, Sebastian, Mishima, Daisuke, Aoki, Takeshi, and Hirose, Shigeo
- Abstract
There is a strong demand for efficient lifesaving techniques and devices in preparation for large-scale earthquakes. We focus on searching survivors and develop the rescue robot "Pneumatic-Drive Expandable Arm." That is an elastic arm type robot driven by pneumatic pressure and has a camera on the head. That can travel stably in the rubble-strewn environment where electric power or wireless communication is not available. [ABSTRACT FROM AUTHOR]
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- 2006
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30. Utility of Early Post-treatment Single-Photon Emission Computed Tomography Imaging to Predict Outcome in Stroke Patients Treated with Intravenous Tissue Plasminogen Activator.
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Abumiya, Takeo, Katoh, Masahito, Moriwaki, Takuya, Yoshino, Masami, Aoki, Takeshi, Imamura, Hiroyuki, Aida, Toshimitsu, Scichinohe, Hideo, Nakayama, Naoki, Kazumata, Ken, and Houkin, Kiyohiro
- Abstract
It is important to predict the outcome of tissue plasminogen activator (tPA)–treated patients early after the treatment for considering the post-tPA treatment option. We assessed cerebral blood flow (CBF) of tPA-treated patients with single-photon emission computed tomography (SPECT) 1 hour after tPA infusion to predict the patient outcome. Technetium-99m-hexamethylpropyleneamine oxime SPECT was performed in 35 consecutive tPA-treated patients. Asymmetry index, a contralateral-to-ipsilateral ratio of CBF, was calculated to analyze CBF quantitatively. Hypoperfusion or hyperperfusion was defined as a decrease of 25% or more or a increase of 25% or more in asymmetry index, respectively. Of all 35 patients, 23 had only hypoperfusion, 8 had both hypoperfusion and hyperperfusion, 2 had only hyperperfusion, and 2 had no perfusion abnormality. When evaluating the association between hypoperfusion and outcome, hypoperfusion volumes were significantly correlated with the modified Rankin Scale at 3 months (r = .634, P < .001). Hyperperfusion was observed in 10 patients (28.6%) and they showed a marked National Institutes of Health Stroke Scale score improvement in the first 24-hour period, which were significantly greater than those of 25 patients without hyperperfusion (P = .033). Eight patients (22.9%) with intracerebral hemorrhage (ICH) were all asymptomatic. Most ICHs were located in hypoperfusion areas, and no ICH was related to hyperperfusion. The results of the present study demonstrated that hypoperfusion volume was associated with poor outcome, whereas the presence of hyperperfusion seemed to be predictive of symptom improvement but not of development of ICH. Taken together, early post-treatment SPECT imaging seems to be a useful biomarker of outcome in tPA-treated patients. [Copyright &y& Elsevier]
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- 2014
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31. Nerve Growth Factor Improves Survival and Function of Transplanted Islets Via TrkA-mediated Cell Proliferation and Revascularization
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Hata, Tatsuo, Sakata, Naoaki, Yoshimatsu, Gumpei, Tsuchiya, Haruyuki, Fukase, Masahiko, Ishida, Masaharu, Aoki, Takeshi, Katayose, Yu, Egawa, Shinichi, and Unno, Michiaki
- Abstract
Nerve growth factor (NGF), which plays important roles in promoting growth and differentiation of nerve cells, has recently been reported as a regulator in pancreatic cells in terms of insulin releasing function. In this study, we examined whether NGF stimulation would promote islet graft survival and function in islet transplantation.
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- 2015
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32. 外地引揚者収容と戦後開拓農民の送出——長野県下伊那郡伊賀良村の...
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Aoki Takeshi
- Abstract
Copyright of Socio-Economic History / Shakai-Keizai Shigaku is the property of Socio-Economic History Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2011
33. Efficacy of Carotid Artery Stenting Performed under General Anesthesia with Somatosensory Evoked Potential Monitoring.
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Gekka, Masayuki, Osanai, Toshiya, Aoki, Takeshi, Nakayama, Naoki, Kazumata, Ken, Houkin, Kiyohiro, and Fujimura, Miki
- Abstract
Objectives: During carotid artery stenting (CAS), hemodynamics may be affected by the carotid sinus reflex in some cases. Although general anesthesia has been reported to stabilize intraoperative hemodynamics, the patient's neurological condition must be assessed indirectly. Therefore, we investigated the changes in intraoperative hemodynamics and perioperative complications of CAS under general anesthesia and evaluated the efficacy of somatosensory evoked potential (SEP) monitoring in detecting a reduction in perfusion during CAS.Materials and Methods: From April 2011 to August 2016,57 consecutive patients who underwent CAS under general anesthesia were evaluated. The follow-up period ranged from 3 to 8 years. During CAS, anesthesiologists monitored and managed the hemodynamics. SEP monitoring was performed during the CAS procedure in all patients.Results: Intraoperative hypotension (systolic blood pressure ≤ 100 mmHg) was evident in 16 patients (28.1%), and 13 patients (22.8%) experienced intraoperative bradycardia; however, all of these cases were promptly managed under general anesthesia. None of the patients showed systolic blood pressure < 50 mmHg from baseline. Regarding perioperative complications, none of the patients exhibited myocardial infarction or postoperative hyperperfusion symptoms, and there was no mortality. Among 21 patients (36.8%) with a decrease in the intraoperative SEP, 3 (5.3%) exhibited transient ischemic symptoms and 1 (1.8%) had postoperative infarction.Conclusions: CAS under general anesthesia is a safe and effective management option in terms of intraoperative hemodynamic stability. In addition, our findings indicate that SEP monitoring could be helpful in evaluating transient postoperative cerebral ischemia or cerebral infarction after CAS. [ABSTRACT FROM AUTHOR]- Published
- 2021
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34. Minimally invasive treatment of stomach cancer.
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Oisuka K, Murakami M, Aoki T, Tajima Y, Kaetsu T, Lefor AT, Otsuka, Koji, Murakami, Masahiko, Aoki, Takeshi, Tajima, Yusuke, Kaetsu, Tsutomu, and Lefor, Alan T
- Abstract
The rate of detection of early gastric cancer has increased because of the development of diagnostic techniques, such as endoscopy, biopsy, and endoscopic ultrasonography. Recently, minimally invasive surgical procedures for benign gastric conditions have been advocated, and the laparoscopic approach is noted as a technique that increases the quality of life. However, the development of laparoscopic gastric resections and laparoscopically assisted gastric operations for malignancy still deserve a word of caution. Laparoscopic local resection of the stomach is used to treat mucosal cancer without lymph node metastasis, and laparoscopy-assisted distal gastrectomy is used to treat early gastric cancer with lymph node metastasis in the perigastric portion. According to short-term results reported by a small group of surgeons, laparoscopic approaches for gastric cancer result in a minimally invasive approach, early recovery, and decreased morbidity and mortality. However, the longterm results of these less invasive treatments are not known in advanced gastric cancer. If the results of randomized controlled studies for advanced gastric cancer are confirmed, the use of these techniques will spread worldwide and may become a standard technique for the resection of gastric cancer. [ABSTRACT FROM AUTHOR]
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- 2005
35. Minimally Invasive Treatment of Stomach Cancer.
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Oisuka, Koji, Murakami, Masahiko, Aoki, Takeshi, Tajima, Yusuke, Kaetsu, Tsutomu, and Lefor, Alan T.
- Subjects
CANCER treatment ,ENDOSCOPY ,GASTRECTOMY ,DIAGNOSTIC ultrasonic imaging ,ENDOSCOPIC ultrasonography ,ULTRASONIC imaging - Abstract
The rate of detection of early gastric cancer has increased because of the development of diagnostic techniques, such as endoscopy, biopsy, and endoscopic ultrasonography. Recently, minimally invasive surgical procedures for benign gastric conditions have been advocated, and the laparoscopic approach is noted as a technique that increases the quality of life. However, the development of laparoscopic gastric resections and laparoscopically assisted gastric operations for malignancy still deserve a word of caution. Laparoscopic local resection of the stomach is used to treat mucosal cancer without lymph node metastasis, and laparoscopy- assisted distal gastrectomy is used to treat early gastric cancer with lymph node metastasis in the perigastric portion. According to short-term results reported by a small cancer result in a minimally invasive approach, early recovery, and decreased morbidity and mortality. However, the longterm results of these less invasive treatments are not known in advanced gastric cancer. If the results of randomized controlled studies for advanced gastric cancer are confirmed, the use of these techniques will spread worldwide and may become a standard technique for the resection of gastric cancer. [ABSTRACT FROM AUTHOR]
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- 2005
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36. Ultrasound with Electromagnetic Tracking Navigation and Image Fusion System in Laparoscopic Liver Surgery: An Initial Clinical Experience
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Aoki, Takeshi, Murakami, Masahiko, Koizumi, Tomotake, Fujimori, Akira, Enami, Yuta, Kusano, Tomokazu, Matsuda, Kazuhiro, Yamada, Kosuke, Nogaki, Koji, Tashiro, Yoshihiko, Wada, Yusuke, Hakozaki, Tomoki, Tomioka, Kodai, Goto, Satoru, Watanabe, Makoto, and Otsuka, Koji
- Published
- 2016
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37. Somatostatin Analog Inhibits the Growth of Insulinoma Cells by p27-Mediated G1 Cell Cycle Arrest
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Aoki, Takeshi, Motoi, Fuyuhiko, Sakata, Naoaki, Naitoh, Takeshi, Katayose, Yu, Egawa, Shinichi, Miyazaki, Jun-ichi, and Unno, Michiaki
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Although the somatostatin analog octreotide (OCT) has been used for uncontrollable insulinoma, the mechanism involved is still unknown. The aim of this study was to elucidate the therapeutic effect of OCT for insulinoma.
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- 2014
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38. Holography-guided percutaneous puncture technique for selective near-infrared fluorescence-guided laparoscopic liver resection using mixed-reality wearable spatial computer
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Aoki, Takeshi, Koizumi, Tomotake, Sugimoto, Maki, and Murakami, Masahiko
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•Accurate segment boundary visualization is crucial for anatomical liver resection.•Indocyanine green (ICG) fluorescence imaging is a practical option for liver mapping.•ICG injection into cancer-bearing portal pedicles can be used for liver mapping.•This procedure is a significant challenge under pneumoperitoneum conditions.•A holographic display supports ICG liver mapping as valuable guidance.
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- 2020
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39. Imaging of transplanted islets by positron emission tomography, magnetic resonance imaging, and ultrasonography
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Sakata, Naoaki, Yoshimatsu, Gumpei, Tsuchiya, Haruyuki, Aoki, Takeshi, Mizuma, Masamichi, Motoi, Fuyuhiko, Katayose, Yu, Kodama, Tetsuya, Egawa, Shinichi, and Unno, Michiaki
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While islet transplantation is considered a useful therapeutic option for severe diabetes mellitus (DM), the outcome of this treatment remains unsatisfactory. This is largely due to the damage and loss of islets in the early transplant stage. Thus, it is important to monitor the condition of the transplanted islets, so that a treatment can be selected to rescue the islets from damage if needed. Recently, numerous trials have been performed to investigate the efficacy of different imaging modalities for visualizing transplanted islets. Positron emission tomography (PET) and magnetic resonance imaging (MRI) are the most commonly used imaging modalities for this purpose. Some groups, including ours, have also tried to visualize transplanted islets by ultrasonography (US). In this review article, we discuss the recent progress in islet imaging.
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- 2013
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40. Postoperative Cholestasis and Cholangitis After Total Pancreatectomy with Biliary Reconstruction Impair the Function of Autotransplanted Islets
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Hata, Tatsuo, Sakata, Naoaki, Aoki, Takeshi, Yoshimatsu, Gumpei, Tsuchiya, Haruyuki, Hayashi, Hiroki, Motoi, Fuyuhiko, Goto, Masafumi, Katayose, Yu, Egawa, Shinichi, and Unno, Michiaki
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- 2013
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41. Repeated Pancreatectomy for Metachronous Duodenal and Pancreatic Metastases of Renal Cell Carcinoma
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Hata, Tatsuo, Sakata, Naoaki, Aoki, Takeshi, Yoshida, Hiroshi, Kanno, Atsushi, Fujishima, Fumiyoshi, Motoi, Fuyuhiko, Masamune, Atsushi, Shimosegawa, Tooru, and Unno, Michiaki
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AbstractA 50-year-old woman had undergone left nephrectomy for renal cell carcinoma 13 years previously. Ten years later, a solitary metastatic tumor had been detected in the pancreatic tail and she had undergone subsequent resection of the pancreatic tail and spleen. Three years after surgery, she was admitted to our hospital for severe anemia resulting from gastrointestinal tract bleeding. Esophagogastroduodenoscopy revealed a 3-cm solid tumor at the oral side of the papilla of Vater. Histology of the bioptic duodenal tissue revealed inflammatory granulation without malignancy. Computed tomography showed a well-contrasted hypervascular tumor in the descending portion of the duodenum. We diagnosed the patient with metachronous duodenal metastasis of renal cell carcinoma and performed a pancreaticoduodenectomy. An ulcerated polypoid mass was detected at the oral side of the papilla of Vater. Histology revealed clear cell carcinoma coated by granulation tissue across the surface of the tumor. Immunohistology demonstrated that the cells were positive for vimentin, CD10 and epithelial membrane antigen and negative for CK7. After a repeated pancreatectomy, the patient had no symptoms of gastrointestinal bleeding and maintained good glucose tolerance without insulin therapy because the remnant pancreas functioned well. In conclusion, for the diagnosis of patients who have previously undergone nephrectomy and present with gastrointestinal bleeding, the possibility of metastasis to the gastrointestinal tract, including the duodenum, should be considered. With respect to surgical treatment, the pancreas should be minimally resected to maintain a free surgical margin during the first surgery taking into account further metachronous metastasis to the duodenum and pancreas.© 2013 S. Karger AG, Basel
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- 2013
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42. Novel Application of Crystalline Indium-Gallium-Zinc-Oxide Technology to LSI: Dynamically Reconfigurable Programmable Logic Device Based on Multi-Context Architecture
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Okamoto, Yuki, Nakagawa, Takashi, Aoki, Takeshi, Ikeda, Masataka, Kozuma, Munehiro, Osada, Takeshi, Kurokawa, Yoshiyuki, Ikeda, Takayuki, Yamade, Naoto, Okazaki, Yutaka, Miyairi, Hidekazu, Koyama, Jun, and Yamazaki, Shunpei
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A pass gate (PG) configured with a configuration memory (CM) composed of a c-axis aligned crystalline In-Ga-Zn-O (CAAC-IGZO) FET and a pass transistor composed of an NMOS FET is applied to a multi-context routing switch (MC-RS) of a dynamically reconfigurable programmable logic device (DRPLD) based on multi-context architecture (MC-DRPLD). The proposed PG (OS PG) requires fewer transistors than a conventional PG configured with an SRAM cell served as a CM (SRAM PG). A MC-RS configured with the OS PG (OS MC-RS) has enabled its layout area to be 40% less than that of a MC-RS configured with the SRAM PG (SRAM MC-RS). Further, the delays of the MC-RSs with 2 to 16 contexts have been reduced by 24% to 37%. The MC-DRPLD including the OS MC-RS can switch contexts in one clock (50 nsec) at a clock frequency of 20 MHz, which enables dynamic reconfiguration at high speed.
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- 2013
43. Preparation of Aqueous Dispersion of Titanium Dioxide Nanoparticles using Plasma on Liquid Surface
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Ito, Michiko, Hayakawa, Masahiro, Takashima, Seigo, Asami, Etsuo, Aoki, Takeshi, Oka, Masato, Asano, Hiroyuki, Kitahara, Michiro, Nakata, Satoru, Yamaguchi, Koichi, and Murase, Yoshiaki
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A method for preparing an aqueous dispersion of titanium dioxide (TiO2) nanoparticles by generating plasma on the liquid surface was developed. The plasma was generated between the tip of a needle electrode in the gas phase and the liquid surface. A 0.01 wt % aqueous dispersion of TiO2was prepared by plasma treatment with ultrasonication. Dynamic light scattering measurements indicated that the average TiO2nanoparticle sizes in the dispersions with plasma treatment in air and Ar atmosphere were approximately 150 and 180 nm, respectively. Although the pH of the dispersion prepared by Ar plasma treatment was fairly close to the isoelectric point of TiO2, the dispersion maintained a finely dispersed state. The surface potentials of TiO2nanoparticles in the dispersions treated with plasma were confirmed to be positively charged. This suggests that the dispersions formed by plasma treatment were stabilized by electrostatic repulsion between the particles.
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- 2012
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44. Recent Patents on Positive Allosteric Modulators of the Metabotropic Glutamate 5 Receptor as a Potential Treatment for Schizophrenia
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Kanuma, Kosuke, Aoki, Takeshi, and Shimazaki, Youichi
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The glutamate hypothesis of schizophrenia suggests that hypofunction of N-methyl-D-aspartate (NMDA) receptors may be critical for schizophrenic symptoms; therefore, pharmacological approaches that enhance NMDA function may be beneficial for the treatment of schizophrenia. Several lines of evidence indicate that NMDA and metabotropic glutamate (mGlu) 5 receptors are closely associated signaling partners and that a selective mGlu5 receptor agonist might provide a viable approach for increasing NMDA receptor function in the treatment of schizophrenia. The orthosteric binding sites across members of the mGlu receptor subtype for a particular endogenous ligand are often highly conserved, making it difficult to achieve high selectivity for the specific mGlu5 receptor. The advanced currents of drug discovery have developed multiple highly selective allosteric modulators of mGlu5 receptor. In the present review, we provide an update of the recent patents and development of positive allosteric modulators of the mGlu5 receptor and explore their therapeutic potential for schizophrenia.
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- 2010
45. Synchronous and Metachronous Extrapancreatic Malignant Neoplasms in Patients with Intraductal Papillary-Mucinous Neoplasm of the Pancreas
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lshida, Masaharu, Egawa, Shinichi, Kawaguchi, Kei, Aoki, Takeshi, Sakata, Naoaki, Mikami, Yukio, Motoi, Fuyuhiko, Abe, Tadayoshi, Fukuyama, Shoji, Katayose, Yu, Sunamura, Makoto, Unno, Michiaki, Moriya, Takuya, Horii, Akira, and Furukawa, Toru
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Background/Aims:Patients with intraductal papillary-mucinous neoplasm (IPMN) of the pancreas are likely to have a better prognosis than those with conventional pancreatic ductal adenocarcinoma. Recently there have been some reports on extrapancreatic malignant neoplasms (EPM) occurring in patients with IPMN. The purpose of this study was to discover the characteristic features of IPMN with EPM compared with IPMN without EPM. Methods:61 patients with IPMN who underwent surgery at Tohoku University Hospital between 1988 and 2006 were retrospectively analyzed. Results:The 61 patients with IPMN in this study comprised 25 with intraductal papillary-mucinous adenomas (IPMA) and 36 with intraductal papillary-mucinous carcinomas (IPMC) including 6 with invasive carcinomas. Synchronous and metachronous EPM were observed in 15 out of the 61 patients (24.6%). Three of these patients, including 2 with IPMA and 1 with invasive carcinoma associated with IPMC, died of the EPM. None of the features, including sex, age, smoking, family history, macroscopic types (main duct type or branch duct type), histological types (gastric, intestinal, pancreatobiliary or oncocytic), and aberrant expression of molecules including CDKN2A, TP53, SMAD4 and DUSP6, except for the histological diagnoses were associated with the occurrence of EPM, i.e., the EPM occurred more often in patients with IPMA (10 out of 25) than in those with IPMC (5 out of 36) in our series (p = 0.0199 by the ?2test, p = 0.0330 by Fisher's exact probability test, p = 0.0422 by Yates' correction). Conclusion:Patients with IPMA were more likely to have EPM than those with IPMC. Patients with IPMA are usually expected to have a fair prognosis but EPM could be fatal in some of them, so it must be noted during follow-up.
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- 2008
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46. Synchronous and Metachronous Extrapancreatic Malignant Neoplasms in Patients with Intraductal Papillary-Mucinous Neoplasm of the Pancreas
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Ishida, Masaharu, Egawa, Shinichi, Kawaguchi, Kei, Aoki, Takeshi, Sakata, Naoaki, Mikami, Yukio, Motoi, Fuyuhiko, Abe, Tadayoshi, Fukuyama, Shoji, Katayose, Yu, Sunamura, Makoto, Unno, Michiaki, Moriya, Takuya, Horii, Akira, and Furukawa, Toru
- Abstract
Background/Aims: Patients with intraductal papillary-mucinous neoplasm (IPMN) of the pancreas are likely to have a better prognosis than those with conventional pancreatic ductal adenocarcinoma. Recently there have been some reports on extrapancreatic malignant neoplasms (EPM) occurring in patients with IPMN. The purpose of this study was to discover the characteristic features of IPMN with EPM compared with IPMN without EPM. Methods: 61 patients with IPMN who underwent surgery at Tohoku University Hospital between 1988 and 2006 were retrospectively analyzed. Results: The 61 patients with IPMN in this study comprised 25 with intraductal papillary-mucinous adenomas (IPMA) and 36 with intraductal papillary-mucinous carcinomas (IPMC) including 6 with invasive carcinomas. Synchronous and metachronous EPM were observed in 15 out of the 61 patients (24.6). Three of these patients, including 2 with IPMA and 1 with invasive carcinoma associated with IPMC, died of the EPM. None of the features, including sex, age, smoking, family history, macroscopic types (main duct type or branch duct type), histological types (gastric, intestinal, pancreatobiliary or oncocytic), and aberrant expression of molecules including CDKN2A, TP53, SMAD4 and DUSP6, except for the histological diagnoses were associated with the occurrence of EPM, i.e., the EPM occurred more often in patients with IPMA (10 out of 25) than in those with IPMC (5 out of 36) in our series (p = 0.0199 by the χ2test, p = 0.0330 by Fisher’s exact probability test, p = 0.0422 by Yates’ correction). Conclusion: Patients with IPMA were more likely to have EPM than those with IPMC. Patients with IPMA are usually expected to have a fair prognosis but EPM could be fatal in some of them, so it must be noted during follow-up. and IAP
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- 2008
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47. Characteristic Clinicopathological Features of the Types of Intraductal Papillary-Mucinous Neoplasms of the Pancreas
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Ishida, Masaharu, Egawa, Shinichi, Aoki, Takeshi, Sakata, Naoaki, Mikami, Yukio, Motoi, Fuyuhiko, Abe, Tadayoshi, Fukuyama, Shoji, Sunamura, Makoto, Unno, Michiaki, Moriya, Takuya, Horii, Akira, and Furukawa, Toru
- Abstract
Intraductal papillary-mucinous neoplasm (IPMN) of the pancreas encompasses a spectrum of neoplasms with both morphological and immunohistochemical variations of mucin glycoproteins. Recently, a consensus nomenclature and criteria were histologically defined for classifying these variants of IPMNs into gastric, intestinal, pancreatobiliary, and oncocytic types. The purpose of this study was to determine associations between the histological types and clinicopathological features in patients with IPMN.
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- 2007
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48. Long-Term Maintenance of the Drug Transport Activity in Cryopreservation of Microencapsulated Rat Hepatocytes
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Koizumi, Tomotake, Aoki, Takeshi, Kobayashi, Yasuna, Yasuda, Daisuke, Izumida, Yoshihiko, Jin, Zhenghao, Nishino, Nobukazu, Shimizu, Yoshinori, Kato, Hirohisa, Murai, Noriyuki, Niiya, Takashi, Enami, Yuta, Mitamura, Keitaro, Yamamoto, Toshinori, and Kusano, Mitsuo
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Transplantation of isolated hepatocytes has been proposed to compensate for essential functions lacking in liver failure or for genetic defects that alter a specific liver metabolic pathway. Hepatocyte utilization for these purposes would be facilitated with a reliable, reproducible, and effective method of long-term hepatocyte storage. We have recently developed a simple new system for cryopreservation of hepatocytes that encapsulates alginate microspheres and maintains liver-specific function. The aim of this study was to elucidate the transport and drug-metabolizing enzyme activities of cryopreserved microencapsulated hepatocytes stored for a long time. Morphological examinations showed there is no apparent injury of the hepatocytes during cryopreservation processes. A drug-metabolizing enzyme (testosterone 6β-hydroxylase, a specific probe for CYP3A2) and drug transport activities [salicylate, allopurinol, and prostaglandin E2(PGE2), typical substrates of rOat2] in cryopreserved microencapsulated hepatocytes were maintained up to 120 days. Our results thus demonstrate for the first time that cryopreservation of primary rat hepatocytes by the encapsulation technique allows long-term retention of drug metabolism and drug transport activities.
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- 2007
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49. A Novel Method of Cryopreservation of Rat and Human Hepatocytes by Using Encapsulation Technique and Possible Use for Cell Transplantation
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Aoki, Takeshi, Koizumi, Tomotake, Kobayashi, Yasuna, Yasuda, Daisuke, Izumida, Yoshihiko, Jin, Zhenghao, Nishino, Nobukazu, Shimizu, Yoshinori, Kato, Hirohisa, Murai, Noriyuki, Niiya, Takashi, Enami, Yuta, Mitamura, Keitaro, Yamamoto, Toshihiro, and Kusano, Mitsuo
- Abstract
Encapsulated hepatocyte transplantation is a promising approach to cell transplantation without immunosuppression as an alternative to whole organ liver transplantation. However, the shortage of donor cells for hepatocyte transplantation has not been resolved, and at this critical point, it seems necessary to establish a method of hepatocyte cryopreservation to allow clinical application of hepatocyte transplantation and the development of a bioartificial liver system in the near future. In this study we demonstrated that cryopreserved microencapsulated rat and human hepatocytes can retain their hepatic function and that cryopreserved microencapsulated human hepatocytes transplanted into rat spleen remain viable without immunosuppression. Rat and human hepatocytes were isolated by a collagenase digestion method, and they were microencapsulated with poly-L-lysine. The microencapsulated rat hepatocytes were transferred to culture medium (DMEM containing 10% FBS and 10% DMSO) and immediately frozen in liquid nitrogen. A warm water bath (37°C) was used to thaw the microencapsulated hepatocytes. Hepatic function, drug metabolism, and cell morphology were assessed after 90 days of cryopreservation. After 1 week of cryopreservation, microencapsulated hepatocytes were cultured for up to 2 weeks to assess their hepatic function and morphology. The morphology of human hepatocytes was assessed after 30 days of cryopreservation. Cryopreserved human hepatocytes were transplanted into rat spleen to assess their morphology. Cryopreserved microencapsulated hepatocytes retained their viability and were strongly positive for expression of albumin, OAT2, CYP3A2, and CYP3A9. Two weeks after cultivation, the cryopreserved microencapsulated rat hepatocytes had retained their hepatic function (urea synthesis). Cryopreserved microencapsulated human hepatocytes also mainly survived and retained their hepatic function for at least 30 days after cryopreservation. Moreover, entrapped cryopreserved human hepatocytes also survived and expressed albumin in rat spleen after transplantation. We demonstrated a novel method of long-term cryopreservation of rat and human hepatocytes by using an encapsulation technique, with retention of biological activity and excellent survival of the cryopreserved microencapsulated human hepatocytes transplanted into rat spleen. We believe that this novel approach to hepatocytes cryopreservation provides a new direction in encapsulated cell therapy with the goal of clinical application in the near future.
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- 2005
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50. Intrasplenic Transplantation of Encapsulated Genetically Engineered Mouse Insulinoma Cells Reverses Streptozotocin-Induced Diabetes in Rats
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Aoki, Takeshi, Hui, Hongxiang, Umehara, Yutaka, Licalzi, Sergio, Demetriou, Achilles A., Rozga, Jacek, and Perfetti, Riccardo
- Abstract
Pancreatic islet transplantation is limited by shortage of donor organs. Although β-cell lines could be used, their secretion of insulin is characteristically glucose independent and immunoisolation is required. Here we show that intrasplenic transplantation of encapsulated glucose-responsive mouse insulinoma cells reversed streptozotocin (STZ)-induced diabetes in rats. MIN-6 cells derived from a transgenic mouse expressing SV 40 large T antigen in pancreatic β-cells were transfected with minigene encoding for human glucagon-like-peptide-1 under the control of rat insulin promoter. The cells were encapsulated in alginate/poly-L-lysine and used for cell transplantation in STZ-diabetic rats. Rats with nonfasting blood glucose (n-FBG) greater than 350 mg/dl were used. In group I rats (n = 6) 20 million encapsulated cells were injected into the spleen. Group II rats (n = 6) received empty capsules. n-FBG was measured biweekly. After 4 and 8 weeks, an intraperitoneal glucose tolerance test (IPGTT) was performed in group I; normal rats served as controls. Plasma insulin level was measured every other week (RIA). After 8 weeks, spleens were removed 1 day before sacrifice. In rats transplanted with cells the n-FBG was 100—150 mg/dl until the end of the study. After splenectomy, all cell recipients became diabetic (glucose 400 ± 20 mg/dl). Transplanted rats showed increase in body weight and insulin production (3.3 ± 1.0 ng/ml versus 0.92 ± 0.3 ng/ml; p < 0.01) and had normal IPGTT. Spleens contained capsules with insulin-positive cells. Overall, data from this work indicate that intrasplenic transplantation of xenogeneic encapsulated insulin-producing cells without immunosuppression reversed diabetes in rats. Excellent survival and function of the transplanted cells was due to the fact that the cells were separated from the bloodstream by the immunoisolatory membrane only and insulin was delivered directly to the liver (i.e., in a physiological manner).
- Published
- 2005
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