166 results on '"Minghui HE"'
Search Results
2. Self-Healing Multimodal Flexible Optoelectronic Fiber Sensors
- Author
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Xiaochun Wang, Guangxue Chen, Kaili Zhang, Ren’ai Li, Zihan Jiang, Hongyou Zhou, Jiulin Gan, and Minghui He
- Subjects
General Chemical Engineering ,Materials Chemistry ,General Chemistry - Published
- 2023
3. Increased cross-presentation by dendritic cells and enhanced anti-tumour therapy using the Arp2/3 inhibitor CK666
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Mariana M. S. Oliveira, Roberta D’Aulerio, Tracer Yong, Minghui He, Marisa A. P. Baptista, Susanne Nylén, and Lisa S. Westerberg
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Cancer Research ,Oncology - Abstract
Background Dendritic cell (DC) vaccines for cancer therapy offer the possibility to let the patient’s own immune system kill cancer cells. However, DC vaccines have shown less efficacy than expected due to failure to induce cancer cell killing and by activating T regulatory cells. Methods We tested if inhibition of signalling via WASp and Arp2/3 using the small molecule CK666 would enhance DC-mediated killing of tumour cells in vitro and in vivo. Results Using CK666 during the ex vivo phase of antigen processing of ovalbumin (OVA), murine and human DCs showed decreased phagosomal acidification, indicating activation of the cross-presentation pathway. When compared to untreated DCs, DCs treated with CK666 during uptake and processing of OVA-induced increased proliferation of OVA-specific CD8+ OT-I T cells in vitro and in vivo. Using the aggressive B16-mOVA melanoma tumour model, we show that mice injected with CK666-treated DCs and OVA-specific CD8+ OT-I T cells showed higher rejection of B16 melanoma cells when compared to mice receiving non-treated DCs. This resulted in the prolonged survival of tumour-bearing mice receiving CK666-treated DCs. Moreover, combining CK666-treated DCs with the checkpoint inhibitor anti-PD1 further prolonged survival. Conclusion Our data suggest that the small molecule inhibitor CK666 is a good candidate to enhance DC cross-presentation for cancer therapy.
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- 2023
4. Distinct single-cell immune ecosystems distinguish true and de novo HBV-related hepatocellular carcinoma recurrences
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Shuling Chen, Cheng Huang, Guanrui Liao, Huichuan Sun, Yubin Xie, Changyi Liao, Jianping Wang, Minghui He, Huanjing Hu, Zihao Dai, Xiaoxue Ren, Xuezhen Zeng, Zhilong Lin, Guo-Pei Zhang, Wenxuan Xie, Shunli Shen, Shaoqiang Li, Sui Peng, Dong-Ming Kuang, Qiang Zhao, Dan G Duda, and Ming Kuang
- Subjects
Gastroenterology - Abstract
ObjectiveRevealing the single-cell immune ecosystems in true versus de novo hepatocellular carcinoma (HCC) recurrences could help the optimal development of immunotherapies.DesignWe performed 5’and VDJ single-cell RNA-sequencing on 34 samples from 20 recurrent HCC patients. Bulk RNA-sequencing, flow cytometry, multiplexed immunofluorescence, and in vitro functional analyses were performed on samples from two validation cohorts.ResultsAnalyses of mutational profiles and evolutionary trajectories in paired primary and recurrent HCC samples using whole-exome sequencing identified de novo versus true recurrences, some of which occurred before clinical diagnosis. The tumour immune microenvironment (TIME) of truly recurrent HCCs was characterised by an increased abundance in KLRB1+CD8+T cells with memory phenotype and low cytotoxicity. In contrast, we found an enrichment in cytotoxic and exhausted CD8+T cells in the TIME of de novo recurrent HCCs. Transcriptomic and interaction analyses showed elevated GDF15 expression on HCC cells in proximity to dendritic cells, which may have dampened antigen presentation and inhibited antitumour immunity in truly recurrent lesions. In contrast, myeloid cells’ cross talk with T cells-mediated T cell exhaustion and immunosuppression in the TIME ofde novorecurrent HCCs. Consistent with these findings, a phase 2 trial of neoadjuvant anti-PD-1 immunotherapy showed more responses in de novo recurrent HCC patients.ConclusionTrue and de novo HCC recurrences occur early, have distinct TIME and may require different immunotherapy strategies. Our study provides a source for genomic diagnosis and immune profiling for guiding immunotherapy based on the type of HCC recurrence and the specific TIME.
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- 2023
5. Supplementary Figure from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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Ming Kuang, Zhenwei Peng, Baogang Peng, Jiaming Lai, Dongming Li, Shunli Shen, Shaoqiang Li, Lixia Xu, Jiehui Tan, Zelong Liu, Zihao Dai, Xiangjun Zhou, Xiaoshuang Li, Yifan Ma, Longqing Tang, Yanyan Han, Minghui He, Huanjing Hu, Yubin Xie, Bin Li, Qian Zhou, Han Xiao, Zebin Chen, Wei Wang, Tianhong Su, Xuezhen Zeng, Jie Mei, Wei Hu, Shuling Chen, and Sui Peng
- Abstract
Supplementary Figure from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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- 2023
6. Data from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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Ming Kuang, Zhenwei Peng, Baogang Peng, Jiaming Lai, Dongming Li, Shunli Shen, Shaoqiang Li, Lixia Xu, Jiehui Tan, Zelong Liu, Zihao Dai, Xiangjun Zhou, Xiaoshuang Li, Yifan Ma, Longqing Tang, Yanyan Han, Minghui He, Huanjing Hu, Yubin Xie, Bin Li, Qian Zhou, Han Xiao, Zebin Chen, Wei Wang, Tianhong Su, Xuezhen Zeng, Jie Mei, Wei Hu, Shuling Chen, and Sui Peng
- Abstract
A high rate of recurrence after curative therapy is a major challenge for the management of hepatocellular carcinoma (HCC). Currently, no effective adjuvant therapy is available to prevent HCC recurrence. We designed a personalized neoantigen-loaded dendritic cell vaccine and neoantigen-activated T-cell therapy, and used it as adjuvant therapy to treat 10 patients with HCC who had undergone curative resection or radiofrequency ablation in the first stage of a phase II trial (NCT03067493). The primary outcomes were safety and neoantigen-specific immune response. Disease-free survival (DFS) was also evaluated. The immunotherapy was successfully administered to all the patients without unexpected delay and demonstrated a reasonable safety profile with no grade ≥3 treatment-related side effects reported. Seventy percent of patients generated de novo circulating multiclonal neoantigen-specific T-cell responses. Induced neoantigen-specific immunity was maintained over time, and epitope spreading was observed. Patients who generated immune responses to treatment exhibited prolonged DFS compared with nonresponders (P = 0.012), with 71.4% experiencing no relapse for 2 years after curative treatment. High expression of an immune stimulatory signature, enhanced immune-cell infiltration (i.e., CD8+ T cells), and upregulated expression of T-cell inflammatory gene profiles were found in the primary tumors of the responders. In addition, neoantigen depletion (immunoediting) was present in the recurrent tumors compared with the primary tumors (7/9 vs. 1/17, P = 0.014), suggesting that immune evasion occurred under the pressure of immunotherapy. Our study indicates that neoantigen-based combination immunotherapy is feasible, safe, and has the potential to reduce HCC recurrence after curative treatment.
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- 2023
7. A Repeatable Self-Adhesive Liquid-Free Double-Network Ionic Conductor with Tunable Multifunctionality
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Ziyu Hua, Guangxue Chen, Kai Zhao, Ren’ai Li, and Minghui He
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General Materials Science - Abstract
Liquid-free ionic conductors (LFICs) have promising applications in flexible electronics because most ionic conductors currently suffer from ionic liquid leakage or water evaporation issues. However, it has been a formidable challenge for LFICs to achieve long-term repeated self-adhesion on different substrates, especially on soft biological tissues. Based on the double-network design concept, we first fabricate a series of repeatable self-adhesive liquid-free double-network ionic conductors (SALFDNICs), consisting of stretchable first poly(AA-ChCl)-type supramolecular deep eutectic polymer networks and stiff second polydopamine (PDA) networks, which can maintain sufficient dynamic hydrogen bonds and catechol groups in the ionic conductors by preventing the overoxidation of dopamine, thus balancing the contradiction between adhesion and cohesion in liquid-free ionic conductors. Therefore, SALFDNICs can instantly form various interface interaction forces with multiple substrates (adhesion strength up to 757 N/m) and firmly adhere to various substrates for 20 detachment-reattachment cycles with a reduction in adhesion strength of less than 15%. Furthermore, SALFDNICs also have other comprehensive properties, such as optimum self-healing properties (self-healing efficiency of 90%), good stretchability (strain at break of 1200%), and promising conductivity (2.31 × 10
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- 2022
8. Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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Sui Peng, Shuling Chen, Wei Hu, Jie Mei, Xuezhen Zeng, Tianhong Su, Wei Wang, Zebin Chen, Han Xiao, Qian Zhou, Bin Li, Yubin Xie, Huanjing Hu, Minghui He, Yanyan Han, Longqing Tang, Yifan Ma, Xiaoshuang Li, Xiangjun Zhou, Zihao Dai, Zelong Liu, Jiehui Tan, Lixia Xu, Shaoqiang Li, Shunli Shen, Dongming Li, Jiaming Lai, Baogang Peng, Zhenwei Peng, and Ming Kuang
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Cancer Research ,Carcinoma, Hepatocellular ,Antigens, Neoplasm ,Liver Neoplasms ,Vaccination ,Immunology ,Immunity ,Humans ,Dendritic Cells ,Immunotherapy ,Neoplasm Recurrence, Local ,Cancer Vaccines - Abstract
A high rate of recurrence after curative therapy is a major challenge for the management of hepatocellular carcinoma (HCC). Currently, no effective adjuvant therapy is available to prevent HCC recurrence. We designed a personalized neoantigen-loaded dendritic cell vaccine and neoantigen-activated T-cell therapy, and used it as adjuvant therapy to treat 10 patients with HCC who had undergone curative resection or radiofrequency ablation in the first stage of a phase II trial (NCT03067493). The primary outcomes were safety and neoantigen-specific immune response. Disease-free survival (DFS) was also evaluated. The immunotherapy was successfully administered to all the patients without unexpected delay and demonstrated a reasonable safety profile with no grade ≥3 treatment-related side effects reported. Seventy percent of patients generated de novo circulating multiclonal neoantigen-specific T-cell responses. Induced neoantigen-specific immunity was maintained over time, and epitope spreading was observed. Patients who generated immune responses to treatment exhibited prolonged DFS compared with nonresponders (P = 0.012), with 71.4% experiencing no relapse for 2 years after curative treatment. High expression of an immune stimulatory signature, enhanced immune-cell infiltration (i.e., CD8+ T cells), and upregulated expression of T-cell inflammatory gene profiles were found in the primary tumors of the responders. In addition, neoantigen depletion (immunoediting) was present in the recurrent tumors compared with the primary tumors (7/9 vs. 1/17, P = 0.014), suggesting that immune evasion occurred under the pressure of immunotherapy. Our study indicates that neoantigen-based combination immunotherapy is feasible, safe, and has the potential to reduce HCC recurrence after curative treatment.
- Published
- 2022
9. Self-Adhesive Dry Ionic Conductors Based on Supramolecular Deep Eutectic Polymers
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Kaili Zhang, Ren’ai Li, Guangxue Chen, Xiaochun Wang, and Minghui He
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General Chemical Engineering ,Materials Chemistry ,General Chemistry - Published
- 2022
10. Table S5 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Table S5 shows detailed information of structure variations in the six HCC patients with WGS analysis.
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- 2023
11. Figure S1-S17 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Fig.S1 shows flow chart of enrollment for multifocal HCC patients with anti-PD-1 therapy. Fig.S2 shows CT images of the six HCC patients with two tumors in the liver. Fig.S3 shows purity of tumor content of the six HCC patients with two tumors in the liver. Fig.S4 shows HBV integration sites in the six HCC patients with two tumors in the liver. Fig.S5 shows expression level of TERT in the six HCC patients with two tumors in the liver. Fig.S6 shows landscape of inter-chromosomal translocations and copy number alterations in small and large tumors of four cases. Fig.S7 shows number and types of chromosomal rearrangement in the six HCC patients with two tumors in the liver. Fig.S8 shows copy number aberrations in the six HCC patients with two tumors in the liver. Fig.S9 shows mutational landscape of the six HCC patients with two tumors in the liver. Fig.S10 shows non-silent mutations in the six HCC patients with two tumors in the liver. Fig.S11 shows profile of mutational cancer cell fractions of small and large tumors in the six HCC patients. Fig.S12 shows unsupervised clustering of RNA expression in tumors and adjacent normal liver tissues of 12 multifocal HCC patients. Fig.S13 shows expression level of immune functional genes in small and large tumors of 12 multifocal HCC patients. Fig.S14 shows expression level of immune stimulators in small and large tumors of 12 multifocal HCC patients. Fig.S15 shows expression level of immune inhibitors in small and large tumors of 12 multifocal HCC patients. Fig.S16 shows mutations of immune-related genes in the six HCC patients with two tumor nodules in the liver. Fig.S17 shows images of the small and large tumors before and after anti-PD-1 therapy.
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- 2023
12. Data from Multiomic Analysis Reveals Comprehensive Tumor Heterogeneity and Distinct Immune Subtypes in Multifocal Intrahepatic Cholangiocarcinoma
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Sui Peng, Ming Kuang, Jian Ren, Baogang Peng, Jiaming Lai, Dongming Li, Shaoqiang Li, Shunli Shen, Hong Peng, Qianwen Zeng, Xiaoxue Ren, Jianping Wang, Yuanqi Wang, Changyi Liao, Lijuan Liu, Minghui He, Huanjing Hu, Yuhong Cai, Yubin Xie, and Shuling Chen
- Abstract
Purpose:Targeted therapy and immunotherapy are transforming the treatment approach for intrahepatic cholangiocarcinoma (ICC). However, little is known about the intertumor heterogeneity (ITH) of multifocal ICC and its impacts on patient response to these treatments. We aimed to characterize the immunogenomic and epigenomic heterogeneity of multifocal ICC to guide treatment decision making.Experimental Design:We obtained 66 tumor samples from 16 patients with multifocal ICC and characterized the tumor and immune heterogeneity using whole-exome sequencing, bulk and single-cell RNA sequencing, methylation microarray, and multiplex immunostaining. Patients were divided into high- or low-ITH groups according to the median ITH index. Two independent cohorts were used to validate findings. Responses to anti-PD-1 therapy were assessed.Results:Multifocal ICC presented considerable intertumor genomic, transcriptional, and epigenomic heterogeneity within a patient in high ITH group. The immune profile among multiple tumors within a patient was relatively less heterogeneous in high- or low-ITH group, and consistent responses of multiple tumors to anti-PD-1 immunotherapy were observed. Unsupervised clustering of immune markers identified one low and one high immune subtype, with higher immune cell infiltration, closer tumor–immune cell interactions, and upregulated IFN-signature expression in high-immune subtype. Determining expression levels of CD8B and ICOS facilitated this immune classification and prediction of patient prognosis. Finally, promoter DNA methylation contributed to different immune profiles of two subtypes by regulating immune-gene expression.Conclusions:There is comprehensive heterogeneity in the genome, transcriptome, and epigenome of multifocal ICC. On the basis of the less heterogeneous immune profile of ICC, we suggest an immune classification that stratifies patients' prognosis and may support personalized immunotherapy.
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- 2023
13. Supplementary Data from Multiomic Analysis Reveals Comprehensive Tumor Heterogeneity and Distinct Immune Subtypes in Multifocal Intrahepatic Cholangiocarcinoma
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Sui Peng, Ming Kuang, Jian Ren, Baogang Peng, Jiaming Lai, Dongming Li, Shaoqiang Li, Shunli Shen, Hong Peng, Qianwen Zeng, Xiaoxue Ren, Jianping Wang, Yuanqi Wang, Changyi Liao, Lijuan Liu, Minghui He, Huanjing Hu, Yuhong Cai, Yubin Xie, and Shuling Chen
- Abstract
Supplementary Data from Multiomic Analysis Reveals Comprehensive Tumor Heterogeneity and Distinct Immune Subtypes in Multifocal Intrahepatic Cholangiocarcinoma
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- 2023
14. Table S9 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Table S9 shows potentially functional mutations of immune-related genes in the six HCC patients with WGS analysis.
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- 2023
15. Table S3 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Table S3 shows summary of whole genome sequencing in the six patients with two tumor nodules in the liver.
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- 2023
16. Table S1, Table S2, Table S4, Table S8, Table S10, Table S11 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Table S1 shows clinicopathologic information of 12 multifocal HCC patients. Table S2 shows PCR primers used for validation of HBV integration sites. Table S4 shows HBV integration analysis in HCC patients with WGS data. Table S8 shows P-value of pathways enrichment analysis in the small and large tumors of 12 multifocal HCC patients. Table S10 shows correlation between the number of rearrangements and immune activity. Table S11 shows detailed clinical data of eight patients with anti-PD-1 therapy.
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- 2023
17. Table S6 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
- Author
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Table S6 shows detailed information of copy number alterations in the six HCC patients with WGS analysis.
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- 2023
18. Data from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
- Author
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Purpose:Immune checkpoint inhibitor therapy is emerging as the promising option for patients with advanced hepatocellular carcinoma. We aimed to investigate the heterogeneity of different tumor nodules of the same patient with multifocal hepatocellular carcinomas in response to immunotherapy and its molecular mechanisms.Experimental Design:We attained 45 surgical tumor samples including 33 small and 12 large nodules from 12 patients with multifocal hepatocellular carcinoma and evaluated genomic and immune heterogeneity among tumors through whole-genome sequencing and RNA sequencing. IHC was performed to validate the expression of immune markers. The responses to anti–programmed cell death protein-1 (PD-1) therapy in patients with multifocal hepatocellular carcinoma were evaluated.Results:The small and large tumors within the same patient presented with similar genomic characteristics, indicating their same genomic origin. We further found the small tumors had higher immune cell infiltration including more CD8+ T cells, M1 macrophages, and monocytes as compared with large tumors. Besides, the expression of interferon signature predictive of response to anti–PD-1 therapy was significantly upregulated in the small tumors. Moreover, the immune pathways were more vigorous along with less active proliferation pathways in the small tumors. In keeping with this, we found that small nodules were more sensitive to anti–PD-1 therapy than large nodules in patients with multifocal hepatocellular carcinoma.Conclusions:The small tumors in patients with multifocal hepatocellular carcinoma had higher immune cell infiltration and upregulation of immune pathways as compared with the large tumors, which can partially explain the different responses of small and large tumors in the same case to anti–PD-1 therapy.
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- 2023
19. Table S7 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Table S7 shows detailed information of candidate somatic mutations in the six HCC patients with WGS analysis.
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- 2023
20. Capitaone A-B, Antioxidant and Cytotoxic Activities Phenolic Compounds from Polygonum Capitatum
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Lei He, Fei Zhong, XingJun Chen, Ya-Ru Yang, XueLong Yan, MingHui He, Xu Zhang, Zhu Zeng, Bo Tu, QingDe Long, and Yan Lin
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- 2023
21. Research Status and Progress of Biomass-Based 3D Printing Materials
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Hanyu Zhao, Ying Jia, Guangxue Chen, Minghui He, Junfei Tian, and Qifeng Chen
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- 2023
22. Superhydrophilic three-dimensional porous spent coffee ground reduced palladium nanoparticles for efficient catalytic reduction
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Shaokai Zhang, Guangxue Chen, Minghui He, Zhaohui Yu, Junfei Tian, Huifang Chan, Xiao-Fang Wan, Congcan Shi, Zhangxiong Wu, and Shenghong Sun
- Subjects
Materials science ,Water transport ,Metal Nanoparticles ,Selective catalytic reduction ,Hydrogen Peroxide ,Coffee ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Catalysis ,Nanocellulose ,Biomaterials ,Colloid and Surface Chemistry ,Chemical engineering ,Superhydrophilicity ,Water treatment ,Chemical stability ,Porosity ,Palladium - Abstract
The use of functional biodegradable wastes to treat environmental problems would create minimal extra burden to our environment. In this paper, we propose a sustainable and practical strategy to turn spent coffee ground (SCG) into a multifunctional palladium-loaded catalyst for water treatment instead of going into landfill as solid waste. Bleached delignified coffee ground (D-SCG) has a porous structure and a good capability to reduce Pd (II) to Pd (0). A large amount of nanocellulose is formed on the surface of SCG after bleaching by H2O2, which anchors and disperses the palladium nanoparticles (Pd NPs). The D-SCG loaded with Pd NPs (Pd-D-SCG) is superhydrophilic, which facilitates water transport and thus promotes efficient removal of organic pollutants dissolved in water. Pd-D-SCG exhibits excellent room temperature catalytic activity for the removal of 4-nitrophenol (4-NP) and methylene blue (MB) in water and shows good chemical stability and recyclability in water, with no obvious decrease even after five repeated cycles.
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- 2022
23. 3D printed, environment tolerant all-solid-state capacitive ionic skin
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Yixuan Wu, Ling Cai, Guangxue Chen, Fengzhi Yang, and Minghui He
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Renewable Energy, Sustainability and the Environment ,General Materials Science ,General Chemistry - Abstract
By the photopolymerization 3D printing of photopolymerizable deep eutectic solvents, a stable and sensitive solid-state capacitive ionic skin is reported.
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- 2022
24. 3D Photoprintable Antistatic Materials with Polymerizable Deep Eutectic Solvents
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Jiahui Su, Shaohui Li, Yonglong Chen, Yanyan Cui, and Minghui He
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General Chemical Engineering ,General Chemistry ,Industrial and Manufacturing Engineering - Published
- 2021
25. Dynamic modeling and analysis for a differential modular robot joint with the friction model
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Yukun Liu, Ruiqing Luo, Minghui He, Liang Du, Sheng Bao, Jianjun Yuan, and Weiwei Wan
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- 2022
26. A Parameter Identification Method for Coupled Dynamics of Robotic Manipulator with Differential Modular Joints
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Minghui He, Ruiqing Luo, Yukun Liu, Liang Du, Sheng Bao, Jianjun Yuan, and Weiwei Wan
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- 2022
27. Mechanistic insight of SARS-CoV-2 infection using human hepatobiliary organoids
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Yi Zhao, Xiaoxue Ren, Jing Lu, Minghui He, Zhe Liu, Lina Yi, Mingle Huang, Ming Kuang, Haipeng Xiao, Joseph JY Sung, Xiaoxing Li, Lixia Xu, and Jun Yu
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Gastroenterology - Published
- 2022
28. A smart nanoplatform for enhanced photo-ferrotherapy of hepatocellular carcinoma
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Longguang Tang, Mingjian Ling, Madiha Zahra Syeda, Rui Sun, Minghui He, Qingchun Mu, Xiulong Zhu, Chunming Huang, and Liao Cui
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Histology ,Biomedical Engineering ,Bioengineering ,Biotechnology - Abstract
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Emerging therapies, such as ferroptosis mediated cancer therapy and phototherapy, offer new opportunities for HCC treatment. The combination of multiple treatments is often more effective than monotherapy, but many of the current treatments are prone to serious side effects, resulting in a serious decline in patients’ quality of life. Therefore, the combination therapy of tumor in situ controllable activation will improve the efficacy and reduce side effects for precise treatment of tumor. Herein, we synthesized a GSH-activatable nanomedicine to synergize photothermal therapy (PTT) and ferrotherapy. We utilized a near-infrared dye SQ890 as both an iron-chelating and a photothermal converter agent, which was encapsulated with a GSH-sensitive polymer (PLGA-SS-mPEG), to attain the biocompatible SQ890@Fe nanoparticles (NPs). In the tumor microenvironment (TME), SQ890@Fe NPs showed a GSH-activated photothermal effect that could increase the Fenton reaction rate. Meanwhile, the depletion of GSH could further increase ferroptosis effect. In turn, the increasing radical generated by ferrotherapy could impair the formation of heat shock proteins (HSPs) which could amplify PTT effects by limiting the self-protection mechanism. Overall, the intelligent nanomedicine SQ890@Fe NPs combines ferrotherapy and PTT to enhance the efficacy and safety of cancer treatment through the mutual promotion of the two treatment mechanisms, providing a new dimension for tumor combination therapy.
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- 2022
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29. Research on design of intersection signal light based on artificial intelligence algorithm
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Minghui He
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- 2022
30. Improvement of GCC Congestion Control Algorithm in Streaming Media Transmission
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Minghui He, Yan Yuan, Kang Li, and Han Peng
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- 2022
31. Stiff, Self-Healable, Transparent Polymers with Synergetic Hydrogen Bonding Interactions
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Minghui He, Guangxue Chen, Ren'ai Li, Bin Su, and Kaili Zhang
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chemistry.chemical_classification ,Materials science ,chemistry ,Chemical engineering ,Hydrogen bond ,General Chemical Engineering ,Materials Chemistry ,General Chemistry ,Polymer - Published
- 2021
32. Distinct Single-cell Immune Ecosystems Distinguish True and De Novo HBV-related Hepatocellular Carcinoma Recurrences
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Shuling Chen, Cheng Huang, Guanrui Liao, Huichuan Sun, Yubin Xie, Jianping Wang, Minghui He, Huanjing Hu, Zihao Dai, Xiaoxue Ren, Xuezhen Zeng, Qianwen Zeng, Guopei Zhang, Changyi Liao, Wenxuan Xie, Shunli Shen, Shaoqiang Li, Sui Peng, Dongming Kuang, Qiang Zhao, Dan G. Duda, and Ming Kuang
- Abstract
SummaryRevealing differential tumor immune microenvironment (TIME) characteristics between true versus de novo hepatocellular carcinoma (HCC) recurrence could help optimal development and use of immunotherapies. Here, we studied the TIME of recurrent HBV-related HCCs by 5’and VDJ single-cell and bulk RNA-sequencing, flow cytometry, and multiplexed immunofluorescence. Analyses of mutational profiles, evolutionary trajectories, and clonal architecture using whole-exome sequencing identified de novo versus true recurrences, some of which occurred before clinical diagnosis. The TIME of truly recurrent HCCs was characterized by an increased abundance in KLRB1+CD8+ T cells with memory phenotype and low cytotoxicity. In contrast, we found an enrichment in cytotoxic and exhausted CD8+ T cells in the TIME of de novo recurrent HCCs. Transcriptomic and interaction analyses showed an upregulated GDF15 expression level on HCC cells in proximity to dendritic cells, which may have dampened antigen presentation and inhibited anti-tumor immunity in the TIME of truly recurrent lesions. In contrast, we found that myeloid cells’ crosstalk with T cells mediated T cell exhaustion and immunosuppression in the TIME of de novo recurrent HCC. In conclusion, our results support genomic diagnosis and immune profiling for guiding immunotherapy implementation based on the type of HCC recurrence and TIME.HighlightsTruly recurrent lesions are seeded before primary tumor diagnosis, and that de novo cancer can occur earlier than the clinically used 2-year limit.ScRNA-seq unravels distinct immune ecosystems in true versus de novo HCC recurrences, highlighting the need for different immunotherapy strategies for two types of HCC recurrence.CD8+ T cells in de novo recurrence displayed cytotoxic and exhausted phenotypes while those in truly recurrent lesions showed a memory phenotype with weak cytotoxicity.HCC cells expressing the inhibitory molecule GDF15 were in the proximity of DCs only in truly recurrent lesions.High GDF15 expression level was associated with truly recurrent HCC and worse prognosis.Graphical abstract
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- 2022
33. Three-dimensional Printed Ultrahighly Sensitive Bioinspired Ionic Skin Based on Submicrometer-Scale Structures by Polymerization Shrinkage
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Ling Cai, Bin Su, Guangxue Chen, Minghui He, and Junfei Tian
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Materials science ,Scale (ratio) ,business.industry ,General Chemical Engineering ,Ionic bonding ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Polymerization ,Materials Chemistry ,0210 nano-technology ,business ,Wearable technology ,Shrinkage - Abstract
Ionic skin with a high performance has been extensively explored due to its ability to convert external stimuli into obvious electrical signals for wearable electronics and intelligent detection. H...
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- 2021
34. CD36 and LC3B initiated autophagy in B cells regulates the humoral immune response
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Chikai Zhou, Saikiran K. Sedimbi, Lisa S. Westerberg, Jin Wang, Mikael C. I. Karlsson, Minghui He, Danai Lianoudaki, Marcus J.G.W. Ladds, Shuijie Li, Shan Wang, Chenfei He, and David P. Lane
- Subjects
0301 basic medicine ,CD36 Antigens ,autophagy ,T-Lymphocytes ,ATG5 ,Plasma Cells ,Plasma cell ,03 medical and health sciences ,Mice ,Sequestosome 1 ,Immune system ,medicine ,Animals ,Humans ,education ,Molecular Biology ,B cell ,Cell Proliferation ,education.field_of_study ,B-Lymphocytes ,030102 biochemistry & molecular biology ,biology ,scavenger receptors ,Autophagosomes ,Germinal center ,Cell Differentiation ,Cell Biology ,b cell ,Immunoglobulin Class Switching ,Cell biology ,Immunity, Humoral ,030104 developmental biology ,medicine.anatomical_structure ,Autophagosome membrane ,Antibody response ,biology.protein ,Antibody ,class switching ,Microtubule-Associated Proteins ,Research Article ,Research Paper - Abstract
Scavenger receptors are pattern recognition receptors that recognize both foreign and self-ligands, and initiate different mechanisms of cellular activation, often as co-receptors. The function of scavenger receptor CD36 in the immune system has mostly been studied in macrophages but it is also highly expressed by innate type B cells where its function is less explored. Here we report that CD36 is involved in macro-autophagy/autophagy in B cells, and in its absence, the humoral immune response is impaired. We found that CD36-deficient B cells exhibit a significantly reduced plasma cell formation, proliferation, mitochondrial mobilization and oxidative phosphorylation. These changes were accompanied by impaired initiation of autophagy, and we found that CD36 regulated autophagy and colocalized with autophagosome membrane protein MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3). When we investigated T-cell-dependent immune responses, we found that mice with CD36 deficiency, specifically in B cells, exhibited attenuated germinal center responses, class switching, and antibody production as well as autophagosome formation. These findings establish a critical role for CD36 in B cell responses and may also contribute to our understanding of CD36-mediated autophagy in other cells as well as in B cell lymphomas that have been shown to express the receptor. Abbreviations: AICDA/AID: activation-induced cytidine deaminase; ATG5: autophagy related 5; ATP: adenosine triphosphate; BCR: B-cell receptor; CPG: unmethylated cytosine-guanosine; CQ: chloroquine; DC: dendritic cells; FOB: follicular B cells; GC: germinal center; Ig: immunoglobulin; LPS: lipopolysaccharide; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MFI: mean fluorescence intensity; MZB: marginal zone B cells; NP-CGG: 4-hydroxy-3-nitrophenylacetyl-chicken gamma globulin; OCR: oxygen consumption rate; oxLDL: oxidized low-density lipoprotein; PC: plasma cells; Rapa: rapamycin; SQSTM1/p62: sequestosome 1; SRBC: sheep red blood cells; Tfh: follicular helper T cells; TLR: toll-like receptor.
- Published
- 2021
35. Polymerizable deep eutectic solvent-based mechanically strong and ultra-stretchable conductive elastomers for detecting human motions
- Author
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Guangxue Chen, Ren'ai Li, Minghui He, Jimin Yang, Junfei Tian, and Kaili Zhang
- Subjects
Toughness ,Materials science ,Renewable Energy, Sustainability and the Environment ,02 engineering and technology ,General Chemistry ,Conductivity ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Elastomer ,01 natural sciences ,Flexible electronics ,0104 chemical sciences ,Deep eutectic solvent ,chemistry.chemical_compound ,chemistry ,Ultimate tensile strength ,General Materials Science ,Composite material ,0210 nano-technology ,Electrical conductor ,Eutectic system - Abstract
Conductive elastomers (CEs) with strong mechanical properties have been fabricated and used in flexible electronics. However, the development of CEs with both super-high mechanical strength and extreme stretchability remains challenging. This paper reports on the development of a series of mechanically strong and tough CEs based on photopolymerizable deep eutectic solvents (PDESs) with dense hydrogen bonding interactions. One of these CEs exhibits the highest reported mechanical performance for elastomers, with tensile strength, strain at break and toughness up to 31.21 MPa, 3645% and 615 MJ m−3, respectively. Notably, this CE tolerates puncture and can lift a 10.5 kg weight (9500 times its own weight) without any failure. In addition, the series of CEs also shows excellent transparency (>94% in the visible range), favorable conductivity (0.007–0.04 S m−1), tunable stretchability (strain from 22% to 6164%) and good self-healing capability (electrical healing efficiency of 99% within 0.26 s). In view of the comprehensive properties of the CEs, we demonstrate the practical suitability of CEs by including them in a strain sensor to detect human motion. Based on their extremely facile preparation process and practical functionalities, we believe that CEs will not only contribute new methods for the development of mechanically strong conductors, but also open up novel avenues for flexible electronics.
- Published
- 2021
36. Mechanically tough yet self-healing transparent conductive elastomers obtained using a synergic dual cross-linking strategy
- Author
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Ren'ai Li, Liang Lin, Minghui He, Qiankun Zhang, and Guangxue Chen
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chemistry.chemical_classification ,Materials science ,Polymers and Plastics ,Organic Chemistry ,Supramolecular chemistry ,Bioengineering ,Nanotechnology ,Transparency (human–computer interaction) ,Polymer ,Elastomer ,Biochemistry ,Flexible electronics ,Deep eutectic solvent ,Stress (mechanics) ,chemistry.chemical_compound ,chemistry ,Self-healing - Abstract
Simultaneously achieving transparency, high mechanical strength and self-healing capability in conductive elastomers with simple and green features is a challenge. Here we develop a novel strategy for a tough yet self-healing supramolecular network by introducing Al(III)-carboxyl complexes into photo-polymerizable deep eutectic solvent (PDES). The resulting polymer simultaneously exhibits high transparency, ionic conductivity, and high stretchability. Notably, due to synergetic interactions of the H-bonds and coordination bonds in the polymer matrix, the supramolecular network shows a high stress at break while achieving good self-healing capability. In view of the outstandingly comprehensive properties, we continue to verify the practicability of our prepared materials as strain sensors to monitor external stimuli such as human motions. Our methodology is simple, green and time-saving, and the fundamental design concept derived from the multifunctional PDES will contribute to the development of tough, self-healing transparent materials and flexible electronics.
- Published
- 2021
37. Overactive WASp in X-linked neutropenia leads to aberrant B-cell division and accelerated plasma cell generation
- Author
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E.A. Deordieva, Lieselot Buedts, Lisa S. Westerberg, Chaohong Liu, Roberta D’Aulerio, Julien Record, Mezida B. Saeed, Peter Vandenberghe, Siobhan O. Burns, Lennart Hammarström, Marton Keszei, Larissa Vasconcelos-Fontes, Yu Xia, Chiara Geyer, Mariana M.S. Oliveira, Charlotte Cunningham-Rundles, Anna Shcherbina, Minghui He, Vinicius Cotta-de-Almeida, Lia Gonçalves Pinho, Lena Bohaumilitzky, Meike Thiemann, Dmitry Pershin, Rhaissa Vieira, Joao Pedro Lopes, Xiaodong Zhao, and Adrian J. Thrasher
- Subjects
Neutropenia ,Wiskott–Aldrich syndrome ,Plasma Cells ,Immunology ,Naive B cell ,macromolecular substances ,Plasma cell ,Biology ,primary immunodeficiency ,plasma cells ,Affinity maturation ,Mice ,X-linked neutropenia ,medicine ,Animals ,Humans ,Immunology and Allergy ,B cell ,B-Lymphocytes ,B cells ,Cell growth ,Germinal center ,Genetic Diseases, X-Linked ,WASp ,medicine.disease ,Molecular biology ,Immunoglobulin A ,medicine.anatomical_structure ,Immunoglobulin class switching ,germinal center ,actin ,Cell Division ,Wiskott-Aldrich Syndrome Protein ,IgA - Abstract
BACKGROUND: B-cell affinity maturation in germinal center relies on regulated actin dynamics for cell migration and cell-to-cell communication. Activating mutations in the cytoskeletal regulator Wiskott-Aldrich syndrome protein (WASp) cause X-linked neutropenia (XLN) with reduced serum level of IgA. OBJECTIVE: We investigated the role of B cells in XLN pathogenesis. METHODS: We examined B cells from 6 XLN patients, 2 of whom had novel R268W and S271F mutations in WASp. By using immunized XLN mouse models that carry the corresponding patient mutations, WASp L272P or WASp I296T, we examined the B-cell response. RESULTS: XLN patients had normal naive B cells and plasmablasts, but reduced IgA+ B cells and memory B cells, and poor B-cell proliferation. On immunization, XLN mice had a 2-fold reduction in germinal center B cells in spleen, but with increased generation of plasmablasts and plasma cells. In vitro, XLN B cells showed reduced immunoglobulin class switching and aberrant cell division as well as increased production of immunoglobulin-switched plasma cells. CONCLUSIONS: Overactive WASp predisposes B cells for premature differentiation into plasma cells at the expense of cell proliferation and immunoglobulin class switching. ispartof: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY vol:149 issue:3 pages:1069-1084 ispartof: location:United States status: published
- Published
- 2022
38. FOXO Dictates Initiation of B Cell Development and Myeloid Restriction in Common Lymphoid Progenitors
- Author
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Lucía Peña-Pérez, Shabnam Kharazi, Nicolai Frengen, Aleksandra Krstic, Thibault Bouderlique, Julia Hauenstein, Minghui He, Ece Somuncular, Xiaoze Li Wang, Carin Dahlberg, Charlotte Gustafsson, Ann-Sofie Johansson, Julian Walfridsson, Nadir Kadri, Petter Woll, Marcin Kierczak, Hong Qian, Lisa Westerberg, Sidinh Luc, and Robert Månsson
- Subjects
B-Lymphocytes ,B cell ,specification ,Forkhead Box Protein O1 ,Precursor Cells, B-Lymphoid ,myeloid restriction ,lineage commitment ,Utvecklingsbiologi ,Lymphoid Progenitor Cells ,gene regulation ,Hematopoiesis ,FOXO (forkhead box protein O) ,Developmental Biology - Abstract
The development of B cells relies on an intricate network of transcription factors critical for developmental progression and lineage commitment. In the B cell developmental trajectory, a temporal switch from predominant Foxo3 to Foxo1 expression occurs at the CLP stage. Utilizing VAV-iCre mediated conditional deletion, we found that the loss of FOXO3 impaired B cell development from LMPP down to B cell precursors, while the loss of FOXO1 impaired B cell commitment and resulted in a complete developmental block at the CD25 negative proB cell stage. Strikingly, the combined loss of FOXO1 and FOXO3 resulted in the failure to restrict the myeloid potential of CLPs and the complete loss of the B cell lineage. This is underpinned by the failure to enforce the early B-lineage gene regulatory circuitry upon a predominantly pre-established open chromatin landscape. Altogether, this demonstrates that FOXO3 and FOXO1 cooperatively govern early lineage restriction and initiation of B-lineage commitment in CLPs.SUMMARYCommon lymphoid progenitors co-express the transcription factors FOXO1 and FOXO3. Removing FOXO1 and FOXO3 at this developmental stage results in regained myeloid potential, failed establishment of the early B cell gene regulatory program, and the complete loss of the B cell lineage.
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- 2022
39. Cellulose Nanofiber-Reinforced Ionic Conductors for Multifunctional Sensors and Devices
- Author
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Xiao Feng, Chao Dang, Ming Wang, Xueqiong Yin, Detao Liu, Minghui He, Ren'ai Li, Fanglin Dai, and Haisong Qi
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Materials science ,Compressive Strength ,Nanofibers ,Ionic bonding ,Hydrogels ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Flexible electronics ,0104 chemical sciences ,Conductor ,chemistry.chemical_compound ,Compressive strength ,chemistry ,Bacterial cellulose ,Tensile Strength ,Nanofiber ,Ultimate tensile strength ,Solvents ,General Materials Science ,Composite material ,Cellulose ,0210 nano-technology ,Electrical conductor - Abstract
Ionic conductors are normally prepared from water-based materials in the solid form and feature a combination of intrinsic transparency and stretchability. The sensitivity toward humidity inevitably leads to dehydration or deliquescence issues, which will limit the long-term use of ionic conductors. Here, a novel ionic conductor based on natural bacterial cellulose (BC) and polymerizable deep eutectic solvents (PDESs) is developed for addressing the abovementioned drawbacks. The superstrong three-dimensional nanofiber network and strong interfacial interaction endow the BC-PDES ionic conductor with significantly enhanced mechanical properties (tensile strength of 8 × 105 Pa and compressive strength of 6.68 × 106 Pa). Furthermore, compared to deliquescent PDESs, BC-PDES composites showed obvious mechanical stability, which maintain good mechanical properties even when exposed to high humidity for 120 days. These materials were demonstrated to possess multiple sensitivity to external stimulus, such as strain, pressure, bend, and temperature. Thus, they can easily serve as supersensitive sensors to recognize physical activity of humans such as limb movements, throat vibrations, and handwriting. Moreover, the BC-PDES ionic conductors can be used in flexible, patterned electroluminescent devices. This work provides an efficient strategy for making cellulose-based sustainable and functional ionic conductors which have broad application in artificial flexible electronics and other products.
- Published
- 2020
40. High-strength paper enhanced by chitin nanowhiskers and its potential bioassay applications
- Author
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Congcan Shi, Junfei Tian, Jing Wu, Tang Hua, Minghui He, Guangxue Chen, and Li Dongjian
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Paper ,Materials science ,Surface Properties ,Chitin ,02 engineering and technology ,Biochemistry ,Nanomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,Structural Biology ,Wet strength ,Bioassay ,Surface charge ,Cellulose ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,Temperature ,General Medicine ,021001 nanoscience & nanotechnology ,Nanostructures ,Cellulose fiber ,Chemical engineering ,chemistry ,Biological Assay ,0210 nano-technology - Abstract
In this paper, nanochitin was used as an alternative natural nanomaterial to combine with cellulose fibers for fabricating high-strength paper. Two typical chitin nanowhiskers having contrasting sign of surface charge were compared to evaluate the enhancement performance on paper in details. The results show that nanochitin with positive charges on the surface has a significant effect on the strength properties of the prepared paper, especially on wet strength. When the dosage of chitin nanowhiskers was 2%, the wet strength index was increased to 2.48 N·m/g, which is important for paper-based analytical devices with the common use in liquid analysis. Typical colorimetric glucose assays were successfully performed, suggesting the improved analytical performance on these prepared paper.
- Published
- 2020
41. Autonomous Self-Healing, Antifreezing, and Transparent Conductive Elastomers
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Ting Fan, Ren'ai Li, Guangxue Chen, Minghui He, Bin Su, Junfei Tian, and Kaili Zhang
- Subjects
Materials science ,General Chemical Engineering ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Elastomer ,01 natural sciences ,0104 chemical sciences ,Molecular network ,Self-healing ,Materials Chemistry ,0210 nano-technology ,Electrical conductor - Abstract
It is a challenge to synthesize all-in-one molecular networks that are autonomously self-healable over a wide temperature range (from subzero to high), transparent, stretchable, and conductive. Her...
- Published
- 2020
42. Transparent conductive elastomers with excellent autonomous self-healing capability in harsh organic solvent environments
- Author
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Guangxue Chen, Kaili Zhang, Minghui He, Ting Fan, and Ren'ai Li
- Subjects
Materials science ,Renewable Energy, Sustainability and the Environment ,Organic solvent ,Supramolecular chemistry ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Elastomer ,01 natural sciences ,0104 chemical sciences ,Deep eutectic solvent ,chemistry.chemical_compound ,Monomer ,chemistry ,Self-healing ,General Materials Science ,0210 nano-technology ,Electrical conductor - Abstract
A new type of supramolecular elastomer, photopolymerized from two rationally designed polymerizable deep eutectic solvent (PDES) monomers, was reported. The resultant network was highly transparent, intrinsically conductive, stretchable, and self-healable. In particular, the healing process can even take place in various organic solvents, simultaneously with enhanced adaptability.
- Published
- 2020
43. Tailoring the Pore Surface of 3D Covalent Organic Frameworks via Post‐Synthetic Click Chemistry
- Author
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Cheng Wang, Bo Gui, Xuefen Liu, Ya Zhang, Pohua Chen, Yuanpeng Cheng, Junliang Sun, and Minghui He
- Subjects
Materials science ,Covalent bond ,Click chemistry ,Nanotechnology ,General Chemistry ,Gas separation ,General Medicine ,Surface engineering ,Catalysis ,Characterization (materials science) - Abstract
Three-dimensional covalent organic frameworks (3D COFs) have gained increasing attention for their attractive features. However, the development of 3D COFs is strongly restricted, mainly due to their synthetic difficulty and complicated structure determination. Post-synthetic modification, which can avoid these problems by incorporating functional moieties into a predetermined framework, provides an alternative way to construct 3D COFs with specific functions. Herein, we report the designed synthesis and characterization of a series of highly crystalline 3D COFs with different loadings of ethynyl groups. Notably, these alkyne-tagged 3D COFs provide a platform for targeted anchoring various specific groups onto the pore walls via click reactions. Moreover, the pore surface engineering can accordingly change their properties, for example, the obtained click products exhibited higher CO2 /N2 selectivity. We describe a simple but powerful strategy to build functional 3D COFs, which will certainly advance them for a ranging of interesting applications in the future.
- Published
- 2021
44. Research on the Formulation of Rural Planning from the Perspective of Integration of Agriculture, Culture and Tourism: A Case Study of Guifengshan Village, Macheng City, Hubei Province
- Author
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Rui Wang, Hong Xu, Xuecheng Hou, and Minghui He
- Subjects
Geography ,Agriculture ,business.industry ,Perspective (graphical) ,Regional science ,business ,Tourism - Abstract
With the gradual construction of the land and space planning system and the acceleration of the urban-rural integrated development process, China’s rural land planning and preparation have ushered in substantial innovations, and at the same time, they are also facing huge challenges. Among them, the low utilization rate of rural land and cultural desertification are the crux of the lagging rural development. This paper takes Guifengshan Village in Macheng City, Hubei Province as an example. Through the analysis of the current advantages of the village, the analysis of challenges, and the evaluation of the suitability of land use, this paper proposes to promote the mutual benefit of agriculture, culture and tourism, and promote the revitalization of Guifengshan Village, forming a “agricultural industry + characteristic cultural creation + red ecology”. “Tourism” jointly develops rural planning strategies. It aims to link rural agriculture, tourism and cultural industries closely on the basis of people-oriented and ecological protection, with income generation as the goal of rural revitalization, to achieve the establishment and extension of the industrial chain, and to promote rural revitalization.
- Published
- 2021
45. N6-Methylandenosine-Related lncRNAs Predict Prognosis and Immunotherapy Response in Bladder Cancer
- Author
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Jianwen Zeng, Yuying Zhang, Chonghe Jiang, Weidong Ji, Baoyi Zhu, Yi Cai, Xiaoling Ying, and Minghui He
- Subjects
Cancer Research ,medicine.medical_treatment ,Cell ,Biology ,medicine.disease_cause ,immune response ,lncRNA ,Immune system ,medicine ,tumor microenvironment ,RC254-282 ,Original Research ,Tumor microenvironment ,Bladder cancer ,N6-methyladenosine ,Competing endogenous RNA ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Immunotherapy ,Cell cycle ,medicine.disease ,medicine.anatomical_structure ,Oncology ,Cancer research ,bladder cancer ,prognosis ,Carcinogenesis - Abstract
Both lncRNAs and the N6-methyladenosine (m6A) modification are key regulators of tumorigenesis and innate immunity. However, little is known about the m6A modification of lncRNAs and their clinical and immune relevance in bladder cancer. In this study, we identified m6A-related lncRNAs using Pearson correlation analysis in The Cancer Genome Atlas (TCGA) and the IMvigor210 datasets. Next, univariate Cox regression was performed using the TCGA dataset to filter prognostic m6A-related lncRNAs, which were further subjected to the least absolute shrinkage and selection operator (LASSO) Cox regression to establish a 12 m6A-related lncRNA prognostic score (m6A-LRS). The m6A-LRS was validated in the IMvigor210 dataset. In addition, high m6A-LRS tumors, characterized by decreased tumor mutation load and neoantigen load, showed poorer response to immunotherapy than those with low m6A-LRS in the IMvigor210 dataset. Further, we constructed an m6A-LRS-based nomogram that demonstrated a strong ability to predict overall survival in patients with bladder cancer. Moreover, enrichment analysis revealed that tumor-associated biological processes, oncogenic signaling, and tumor hallmarks were commonly associated with a high m6A-LRS. Gene set variation analysis also indicated that high m6A-LRS was associated with activation of canonical oncogenic signatures, such as the epithelial-to-mesenchymal transition, cell cycle regulators, and DNA replication, as well as activation of immunosuppressive signatures, such as the T-cell exhaustion and pan-fibroblast-TGF-β response signatures. Furthermore, we observed distinct tumor microenvironment cell infiltration characteristics between high- and low-risk tumors. High m6A-LRS tumors showed reduced infiltration of CD8+ T-cells and enhanced infiltration of macrophages and fibroblasts. Additionally, we established a competing endogenous RNA network based on the12 m6A-related lncRNAs. Finally, three lncRNAs (SNHG16, SBF2-AS1, and BDNF-AS) were selected for further validation. The qualitative PCR assay on 10 pairs of bladder cancer and adjacent normal control samples validated the differential expression, and methylated RNA immunoprecipitation (MeRIP) analysis demonstrated a robust m6A enrichment in T24 bladder cancer cells compared with normal uroepithelial cells (SVHUC-1). In conclusion, this study introduced an m6A-related lncRNA signature that identified a subgroup of patients with poor prognoses and suboptimal immune responses, thus providing novel approaches for treatment response prediction and patient stratification in bladder cancer.
- Published
- 2021
46. Relationship between cumulative exposure to metal mixtures and heart rate among Chinese preschoolers
- Author
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Ye Fu, Yun Liu, Yanli Liu, Yan Wang, Meiqin Zhu, Wei Lin, Mingzhu Li, Yang Liu, Minghui He, Lili Yu, and Jing Wang
- Subjects
China ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Pollution ,Arsenic ,Zinc ,Heart Rate ,Metals, Heavy ,Humans ,Environmental Chemistry ,Child - Abstract
The cumulative exposure to metals affects cardiac conduction, and the effect of polymetallic exposure on heart rate in children is unknown. To evaluate the relationship between cumulative exposure to metal mixtures and heart rate among Chinese preschoolers, the determination of urinary 24 metals was processed by high-resolution inductively coupled plasma-mass spectrometry. Heart rate was recorded when measuring blood pressure after resting 5 min or longer. As a method to compute the Environmental risk score (ERS) according to heart rate under heavy metal mixtures, adaptive elastic net (AENET) with 299 predictors which were formed by the combination of main effects, squared terms, and pairwise interactions of heavy metals with a total number of 23, 23, 253 respectively. To further assess the associations between ERS and heart rate, regression analyses were performed with complex survey designs. The construction of ERS under heart rate-related metal mixtures was returned by AENET in according to 11 main effects (tin, arsenic, zinc, iron, titanium, vanadium, nickel, manganese, cobalt, copper and chromium) and 2 squared terms (tungsten and rubidium). A high correlation was monitored between the alteration of ERS in the study population and heart rate (β = 1.030, 95% CI: 0.730 - 1.330 in 1239; β = 1.085, 95% CI: 0.777 - 1.393 in 1061). Significant associations of ERS with higher heart rates were also pointed out (Ps 0.05). Our study elucidates the association of the cumulative exposure of heavy metals as mixtures and heart rate among Chinese preschoolers. Further research is obliged to corroborate these findings in longitudinal studies.
- Published
- 2022
47. Ultrastretchable and Antifreezing Double-Cross-Linked Cellulose Ionic Hydrogels with High Strain Sensitivity under a Broad Range of Temperature
- Author
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Ren'ai Li, Guangxue Chen, Haisong Qi, Fachuang Lu, Junfei Tian, Ruiping Tong, Minghui He, and Danhong Pan
- Subjects
Materials science ,Renewable Energy, Sustainability and the Environment ,General Chemical Engineering ,Natural polymers ,Ionic bonding ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Sensitivity (explosives) ,0104 chemical sciences ,High strain ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Self-healing hydrogels ,Environmental Chemistry ,Ionic conductivity ,Ammonium persulfate ,0210 nano-technology ,Electrical conductor - Abstract
Stretchable and antifreezing conductive hydrogels, especially prepared from natural polymers, are beneficial for important and rapidly growing stretchable electronic devices. Unfortunately, the pot...
- Published
- 2019
48. Preparation of high-density garnet thin sheet electrolytes for all-solid-state Li-Metal batteries by tape-casting technique
- Author
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Yi-Peng Zhang, Kang-Ning Gao, Zhonghui Cui, Xixiang Li, Jun Gao, Minghui He, Yiqiu Li, Tao Zhang, and Zhongliang Zhan
- Subjects
Tape casting ,Materials science ,Mechanical Engineering ,Metals and Alloys ,Sintering ,02 engineering and technology ,Electrolyte ,Thin sheet ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,0104 chemical sciences ,Metal ,Mechanics of Materials ,visual_art ,Materials Chemistry ,visual_art.visual_art_medium ,Relative density ,Ionic conductivity ,Composite material ,0210 nano-technology - Abstract
Garnet electrolyte is a promising candidate for the development of all-solid-state Li-metal batteries, because of its safety and stability against Li metal. However, its practical application is limited by the difficulty in production of its high-density thin sheet. In this work, we have fabricated garnet thin sheet electrolytes (Li6.4La3Zr1.4Ta0.6O12, LLZTO) by tape-casting technique. By using Li2O as a liquid-phase sintering additive, a high relative density up to 99% was achieved. Electrochemical results show the LLZTO sheets have an ionic conductivity of 5.2 × 10−4 S cm−1 at 30 °C and good cycling stability against Li metal. Based on the sheets, all-solid-state Li/LLZTO/LiFePO4 batteries exhibit excellent reversible cycles at 60 °C and 0.1C with an initial discharge specific capacity of 125.8 mA h g−1 and a retention of 92.3% after 50 cycles. These results indicate that the tape-casting process demonstrated here provides an effective method of producing high-density garnet thin sheet electrolytes for high-performance all-solid-state Li-metal batteries.
- Published
- 2019
49. Nanocomposite intermediate layers formed by conversion reaction of SnO2 for Li/garnet/Li cycle stability
- Author
-
Jingming Fu, Xiangxin Guo, Hanyu Huo, Yiqi Li, Fangfang Xu, Minghui He, Yue Chen, Tao Zhang, and Ning Zhao
- Subjects
Chemical substance ,Materials science ,Nanocomposite ,Renewable Energy, Sustainability and the Environment ,Alloy ,Energy Engineering and Power Technology ,02 engineering and technology ,Electrolyte ,engineering.material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,law.invention ,Magazine ,Chemical engineering ,Coating ,law ,Fast ion conductor ,engineering ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,0210 nano-technology ,Science, technology and society - Abstract
Garnets are promising solid electrolytes for developing solid state Li batteries, owing to their features of relatively high conductivity and stability against lithium metal. However, they show shortcoming of Li penetration through garnets during Li plating and stripping, which limits their practice application. Herein, we present a strategy to solve such problem by coating SnO2 films on the surfaces of the Li6·4La3Zr1·4Ta0·6O12 pellets. Through conversion reaction of SnO2 with Li at 200 °C, the nanocomposite layers consisting of crosslinked LixSn and Li2O are formed between the Li and the Li6·4La3Zr1·4Ta0·6O12 electrolytes. This leads to transition from lithiophobicity to lithiophilicity, thus greatly reducing interfacial resistance from 1100 Ω cm2 to 25 Ω cm2. Furthermore, taking advantage of suppressing volume change of LixSn alloy which is about 260%, the intermediate layers maintain integrity under the current densities of 0.2 mA cm−2 for 650 h cycles. In addition, the critical current density of Li/SnO2-Li6.4La3Zr1·4Ta0·6O12-SnO2/Li can be as high as 1.15 mA cm−2. As a proof-of-concept, this effective interface modification based on conversion reaction method contributes to a useful way of solving the Li/garnet interface problem and promoting the solid state Li batteries development.
- Published
- 2019
50. Angle-shaped triboelectric nanogenerator for harvesting environmental wind energy
- Author
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Hongbin Lin, Qingshen Jing, Shutang Wang, Yaoli Zhang, Yannan Xie, Minghui He, Weifeng Yang, Lianhui Wang, Yanwen Ma, Ning Li, Jing Li, and Ying Liu
- Subjects
Wind power ,Materials science ,Renewable Energy, Sustainability and the Environment ,business.industry ,Nanogenerator ,02 engineering and technology ,Electrostatic induction ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Engineering physics ,Wind speed ,0104 chemical sciences ,law.invention ,Capacitor ,law ,General Materials Science ,Electrical and Electronic Engineering ,0210 nano-technology ,Contact electrification ,business ,Contact area ,Triboelectric effect - Abstract
Recently, flutter-driven triboelectric nanogenerator (TENG) has shown huge potential in wind energy harvesting from ambient environments. However, almost all the reported devices are based on parallel structure which suffers from a critical problem of insufficient contact between triboelectric surfaces. In this work, we propose an angle-shaped TENG (AS-TENG) with the above issue resolved. The device is based on two Al layers stacked to form an angle shape and a FEP film positioned in between by sharing a common side. Thanks to this design, the FEP film can contact fully and intimately with the Al layers to facilitate the effect of contact electrification and electrostatic induction. Compared with the existing parallel-structured TENG, the AS-TENG shows an overwhelming output performance because of the enhanced contact area. Furthermore, the AS-TENG is introduced a wedge-shaped wind guide channel which can provide a driving force for the flutter and hence lower the start-up wind speed largely. To further enhance the electric output, the AS-TENGs can be facilely integrated into a 360° radial array, showing an enlarged windward area and a high level of integration. The AS-TENG array can be applied to harvest outdoor weak wind and act as a direct power source to charge a capacitor or drive electronic devices. This work provides a design strategy for natural weak wind scavenging which may push forward the practical applications of TENG for wind power.
- Published
- 2019
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