75 results on '"Dongdong Su"'
Search Results
2. 64Cu radiolabeled nanomaterials for positron emission tomography (PET) imaging
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Xueqian Chen, Wenchao Niu, Zhongying Du, Yong Zhang, Dongdong Su, and Xueyun Gao
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General Chemistry - Published
- 2022
3. Image‐guided diagnosis and treatment of glioblastoma
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Yongning Bian, Yaling Wang, Xueqian Chen, Yong Zhang, Shaoqing Xiong, and Dongdong Su
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General Energy - Published
- 2023
4. Enzyme-activated near-infrared fluorogenic probe with high-efficiency intrahepatic targeting ability for visualization of drug-induced liver injury†
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Yaling Wang, Mingrui Li, Yong Zhang, Xueqian Chen, Xueyun Gao, Dongdong Su, Qing Yuan, and Yongning Bian
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Liver injury ,chemistry.chemical_classification ,Drug ,Fluorophore ,media_common.quotation_subject ,digestive, oral, and skin physiology ,Cholic acid ,General Chemistry ,medicine.disease ,chemistry.chemical_compound ,Chemistry ,Liver targeting ,Enzyme ,chemistry ,In vivo ,medicine ,Biophysics ,Leucine ,human activities ,media_common - Abstract
Hepatotoxicity is a serious problem faced by thousands of clinical drugs, and drug-induced liver injury (DILI) caused by chronic administration or overdose has become a major biosafety issue. However, the near-infrared (NIR) fluorescent probes currently used for liver injury detection still suffer from poor liver targeting ability and low sensitivity. Enzyme-activated fluorogenic probes with powerful in situ targeting ability are the key to improving the imaging effect of liver injury. Herein, we rationally designed a leucine aminopeptidase (LAP) activated fluorogenic probe hCy-CA-LAP, which greatly improved the hepatocyte-targeting capability by introducing a cholic acid group. The probe hCy-CA-LAP is converted into a high-emission hCy-CA fluorophore in the presence of LAP, showing high selectivity, high sensitivity and low detection limit (0.0067 U mL−1) for LAP, and successfully realizes the sensitive detection of small fluctuations of LAP in living cells. Moreover, the probe can achieve effective in situ accumulation in the liver, thereby achieving precise imaging and evaluation of two different types of drug-induced hepatotoxicity in vivo. Therefore, the probe hCy-CA-LAP may be a potential tool for exploring the roles of LAP and evaluating the degree of DILI., We rationally designed a leucine aminopeptidase (LAP) activated fluorogenic probe hCy-CA-LAP with high hepatocyte-targeting ability for accurate and sensitive imaging of DILI.
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- 2021
5. Multi-stage planning of integrated energy systems considering external carbon trading
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Shiyu Ji, Dongdong Su, Qianqian Song, Yibo Li, Pengxu Dong, and Yongli Wang
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- 2022
6. Cell membranes targeted unimolecular prodrug for programmatic photodynamic-chemo therapy
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Dongdong Su, Jie Yuan, Xiao-Bing Zhang, Xiujuan Zhuang, Hepeng Zhao, Xing-Xing Zhang, Mei Chen, Lin Yuan, and Rong Peng
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Combination therapy ,medicine.medical_treatment ,Cell ,Medicine (miscellaneous) ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,two-photon photodynamic therapy ,combination therapy ,In vivo ,medicine ,Animals ,Humans ,Prodrugs ,Photosensitizer ,glutathione ,Hypoxia ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Fluorescent Dyes ,Photons ,Chemotherapy ,Photosensitizing Agents ,Singlet Oxygen ,Staining and Labeling ,Tumor hypoxia ,Rhodamines ,Chemistry ,Cell Membrane ,Mammary Neoplasms, Experimental ,Prodrug ,021001 nanoscience & nanotechnology ,humanities ,0104 chemical sciences ,medicine.anatomical_structure ,Photochemotherapy ,Cancer research ,Female ,0210 nano-technology ,Azo Compounds ,Research Paper ,HeLa Cells - Abstract
Photodynamic therapy (PDT) has emerged as one of the most up-and-coming non-invasive therapeutic modalities for cancer therapy in rencent years. However, its therapeutic effect was still hampered by the short life span, limited diffusion distance and ineluctable depletion of singlet oxygen (1O2), as well as the hypoxic microenvironment in the tumor tissue. Such problems have limited the application of PDT and appropriate solutions are highly demand. Methods: Herein, a programmatic treatment strategy is proposed for the development of a smart molecular prodrug (D-bpy), which comprise a two-photon photosensitizer and a hypoxia-activated chemotherapeutic prodrug. A rhodamine dye was designed to connect them and track the drug release by the fluorescent signal generated through azo bond cleavage. Results: The prodrug (D-bpy) can stay on the cell membrane and enrich at the tumor site. Upon light irradiation, the therapeutic effect was enhanced by a stepwise treatment: (i) direct generation of 1O2 on the cell membrane induced membrane destruction and promoted the D-bpy uptake; (ii) deep tumor hypoxia caused by two-photon PDT process further triggered the activation of the chemotherapy prodrug. Both in vitro and in vivo experiments, D-bpy have exhabited excellent tumor treatment effect. Conclusion: The innovative programmatic treatment strategy provides new strategy for the design of follow-up anticancer drugs.
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- 2021
7. Study on host-guest interaction of aroma compounds/γ-cyclodextrin inclusion complexes
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Xingran Kou, Yaqi Zhang, Dongdong Su, Hui Wang, Xin Huang, Yunwei Niu, Qinfei Ke, Zuobing Xiao, and Qingran Meng
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Food Science - Published
- 2023
8. Mapping the endogenous Zn2+ in situ during zebrafish embryogenesis by a fluorogenic sensor
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Xueqian Chen, Wenchao Niu, Qing Yuan, Yong Zhang, Xueyun Gao, and Dongdong Su
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Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
9. Chemiluminescent Probes Based on 1,2-Dioxetane Structures For Bioimaging
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Yaling Wang, Yongning Bian, Xueqian Chen, and Dongdong Su
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Heterocyclic Compounds, 1-Ring ,Luminescence ,Organic Chemistry ,Luminescent Measurements ,General Chemistry ,Biochemistry ,Fluorescence - Abstract
Chemiluminescent probes based on 1,2-dioxetane scaffold are one of the most sensitive imaging modalities for detecting disease-related biomarkers and can obtain more accurate biological information in cells and in vivo. Due to the elimination of external light excitation, the background autofluorescence problem in fluorescence technology can be effectively avoided, providing ultrahigh sensitivity and signal-to-noise ratio for various applications. In this review, we highlight a comprehensive but concise overview of activatable 1,2-dioxetane-based chemiluminescent probes by reporting significant advances in accurate detection and bioimaging. The design principles and applications for reactive species, enzymes, and other disease-related biomarkers are systematically discussed and summarized. The challenges and potential prospects of chemiluminescent probes are also discussed to further promote the development of new chemiluminescence methods for biological analysis and diagnosis.
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- 2022
10. Inherently PET/CT Dual Modality Imaging Lipid Nanocapsules for Early Detection of Orthotopic Lung Tumors
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Xiangchun Zhang, Pengju Cai, Zhibo Liu, Chunyu Zhang, Liang Gao, Liu Yu, Dongdong Su, Xueyun Gao, Wenjiang Yang, Fuping Gao, Hui Liu, Huaidong Jiang, and Zhesheng He
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medicine.medical_specialty ,PET-CT ,Lung ,business.industry ,Lipid nanocapsules ,Biochemistry (medical) ,Biomedical Engineering ,Cancer ,Early detection ,General Chemistry ,Pet imaging ,medicine.disease ,Biomaterials ,medicine.anatomical_structure ,medicine ,Dual modality ,Radiology ,Stage (cooking) ,business - Abstract
Accurate diagnosis of cancer at an early stage is the key to reduce cancer mortality and improve survival. PET imaging has high sensitivity but low spatial resolution, while CT imaging has good spatial location information. Therefore, the combination of PET and CT imaging can provide complementary advantages to achieve accurate early diagnosis of tumors. However, currently developed PET or CT imaging agents have only a single function. Here, we designed and constructed a self-assembled lipid nanocapsule encapsulated with iodixanol and labeled with self-chelated
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- 2022
11. Peptide and protein modified metal clusters for cancer diagnostics
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Xueyun Gao, Dongdong Su, Xiangchun Zhang, Fuping Gao, and Liang Gao
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Chemistry ,Low toxicity ,Computer science ,Nanotechnology ,General Chemistry ,Materials design ,Metal clusters - Abstract
A perspective on the development of peptide and protein modified metal clusters in cancer diagnostic applications., The biomedical features of metal clusters have been explored in tumor diagnostic applications in recent years. Peptide or protein protected metal clusters with low toxicity, ultra-small size and good biocompatibility are ideal bioanalytical tools, and exhibit better cancer diagnostic properties that have been attractive to oncologists. This perspective provides a rigorous but succinct overview of cancer diagnosis as a working concept for metal clusters by reporting the latest significant advances in the applications of metal clusters in tumor-related bioanalysis and diagnosis. The materials design principles, bioanalytical mechanisms and biomedical applications of metal clusters are described, and then the potential challenges and prospects of metal clusters in cancer diagnosis are discussed. A perspective addressing the role of metal clusters in this field is required to understand their effects and functions, as well as for the scientific community to further advance the development of metal clusters for broader diagnostic applications.
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- 2020
12. Noble-metal nanocluster as enzyme-mimetic catalyst for diagnostic analysis
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Dongdong Su, Qing Yuan, ShiZheng Wang, Xueyun Gao, Liang Gao, and Xiaojun Ren
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chemistry.chemical_classification ,Enzyme ,chemistry ,Diagnostic analysis ,General Engineering ,engineering ,General Materials Science ,Noble metal ,engineering.material ,Combinatorial chemistry ,Catalysis - Published
- 2019
13. Recent advances in molecular fluorescent probes for organic phosphate biomolecules recognition
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Dongdong Su, Shulu Yin, Xiangyang Gong, Wei Li, Lin Yuan, Xiao-Bing Zhang, and Xiaopeng Fan
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chemistry.chemical_classification ,Biomolecule ,Signal modulation ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry ,Nucleic acid ,0210 nano-technology - Abstract
Organic phosphate biomolecules (OPBs) are indispensable components of eukaryotes and prokaryotes, such as acting as the fundamental components of cell membranes and important substrates for nucleic acids. They play pivotal roles in various biological processes, such as energy conservation, metabolism, and signal modulation. Due to the difficulty of detection caused by variety OPBs, investigation of their respective physiological effects in organisms has been restrained by the lack of efficient tools. Many small fluorescent probes have been employed for selective detection and monitoring of OPBs in vitro or in vivo due to the advantages of tailored properties, biodegradability and in situ high temporal and spatial resolution imaging. In this review, we summarize the recent advances in fluorescent probes for OPBs, such as nucleotides, NAD(P)H, FAD/FMN and PS. Importantly, we describe their identification mechanisms in detail and discuss the general strategies for these OPBs probe designs, which provide new insights and ideas for the future probe designs.
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- 2019
14. Activatable fluorogenic probe for accurate imaging of ulcerative colitis hypoxia
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Mingrui Li, Yong Zhang, Xiaojun Ren, Wenchao Niu, Qing Yuan, Kai Cao, Jinchao Zhang, Xueyun Gao, and Dongdong Su
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Diagnostic Imaging ,Dextran Sulfate ,Metals and Alloys ,General Chemistry ,Nitroreductases ,Nitric Oxide ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Up-Regulation ,Disease Models, Animal ,Mice ,Microscopy, Fluorescence ,A549 Cells ,Materials Chemistry ,Ceramics and Composites ,Animals ,Humans ,Colitis, Ulcerative ,Hypoxia ,Fluorescent Dyes - Abstract
An efficient probe NB-NO2 for specific imaging of ulcerative colitis via hypoxia detection was developed.
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- 2021
15. A fast, scalable, and energy-efficient edge acceleration architecture based on FPGA cluster
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Dongdong Su, Hongwei Kan, and Li Rengang
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Acceleration ,Remote direct memory access ,business.industry ,Computer science ,Server ,Embedded system ,Scalability ,Cloud computing ,Enhanced Data Rates for GSM Evolution ,Energy consumption ,business ,Efficient energy use - Abstract
FPGA-based acceleration has been emerged to avoid the cloud computing overload problem by accelerating the compute-intensive workload on edge networks. Though existing studies for FPGA-based edge acceleration have focused on optimizing the computing time, they did not address the burden of the FPGA-aided server under an enormous computing requests circumstance. The massive computing requests can cause delays in transmission and processing at the FPGA-aided server, leading to long response times and high system energy consumption. Therefore, we propose an emerging edge acceleration architecture based on FPGA cluster over the low-latency RDMA-based network. Preliminary simulation results demonstrate that our architecture is fast, scalable, and energy-efficient in comparison with the FPGA-aided servers cluster.
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- 2021
16. HLS Based Ultra-low Latency FAST Protocol Decoder
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Hongwei Kan, Rui Hao, Jiangwei Wang, Guoqiang Mei, Dongdong Su, and Songqing Deng
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- 2021
17. A fast responsive chromogenic and near-infrared fluorescence lighting-up probe for visual detection of toxic thiophenol in environmental water and living cells
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Ruilong Sheng, Juanjuan Wu, Wei Li, Lintao Zeng, João Rodrigues, Caiqin Qin, and Dongdong Su
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Infrared Rays ,02 engineering and technology ,Wastewater ,Photochemistry ,01 natural sciences ,Chloride ,Fluorescence ,Analytical Chemistry ,chemistry.chemical_compound ,Phenols ,Environmental water ,Limit of Detection ,Oxazines ,medicine ,Humans ,Sulfhydryl Compounds ,Fluorescent Dyes ,Detection limit ,Sulfonamides ,Microscopy, Confocal ,Chromogenic ,Thiophenol ,010401 analytical chemistry ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Nile blue ,0104 chemical sciences ,Spectrometry, Fluorescence ,Models, Chemical ,chemistry ,0210 nano-technology ,Selectivity ,Water Pollutants, Chemical ,HeLa Cells ,medicine.drug - Abstract
Thiophenols as high toxic environmental pollutants are poisonous for animals and aquatic organisms. Therefore, it is indispensable to monitor thiophenols in the environment. Herein, a novel near-infrared fluorescent probe was developed for the detection of thiophenols, which was easily prepared by one-step coupling of 2,4-dinitrobenzenesulfonyl chloride with Nile blue. The probe showed a significant near infrared (∼675 nm) fluorescence “turn-on” response to thiophenols with some good features including chromogenic reaction, high sensitivity and selectivity, fast response, near-infrared emission along with low detection limit (1.8 nM). The probe was employed to rapidly and visually determine thiophenols in several industrial wastewaters with good recoveries (90–110%). Moreover, this probe has been demonstrated good capability for imaging thiophenol in HeLa cells.
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- 2019
18. Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
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Florent Ginhoux, Jin-Soo Kim, Young-Tae Chang, Aymeric Silvin, Sungjin Park, H. Shawn Je, Masahiro Fukuda, Audrey Tze Ting Khoo, Xiao Liu, Beomsue Kim, Sejong Choi, Taejoon Kwon, Jungyeol Lee, Xiaogang Liu, Dongdong Su, Weijie Chi, Diana S. Y. Wan, and Srikanta Sanu
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Genetically modified mouse ,Brain development ,Microglia ,010405 organic chemistry ,Chemistry ,Optical Imaging ,Chemical probe ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Catalysis ,In vitro ,0104 chemical sciences ,Mice ,medicine.anatomical_structure ,nervous system ,In vivo ,medicine ,Animals ,Macrophage ,Glucuronosyltransferase ,Neuroscience ,Fluorescent Dyes - Abstract
Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
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- 2019
19. Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
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Beomsue Kim, Masahiro Fukuda, Jung‐Yeol Lee, Dongdong Su, Srikanta Sanu, Aymeric Silvin, Audrey T. T. Khoo, Taejoon Kwon, Xiao Liu, Weijie Chi, Xiaogang Liu, Sejong Choi, Diana S. Y. Wan, Sung‐Jin Park, Jin‐Soo Kim, Florent Ginhoux, H. Shawn Je, and Young‐Tae Chang
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General Medicine - Published
- 2019
20. A distinctive near-infrared fluorescence turn-on probe for rapid, sensitive and chromogenic detection of sulfite in food
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Chong Duan, Guang-Ming Bao, Dongdong Su, Lintao Zeng, Jun-Feng Zhang, and Yubo Hu
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Chromatography ,food.ingredient ,Chromogenic ,Process Chemistry and Technology ,General Chemical Engineering ,Food additive ,food and beverages ,02 engineering and technology ,Near infrared fluorescence ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry.chemical_compound ,Residue (chemistry) ,Visual detection ,food ,chemistry ,Sulfite ,0210 nano-technology ,Sodium sulfite - Abstract
Sodium sulfite (Na2SO3) is widely used as a food additive, but the excessive residue of SO32− in food can cause irritative effects and damages to the human body. In this study, we reported a novel near-infrared (NIR) fluorescent probe (DCMQ) for visual detection of SO32− based on a chromogenic reaction. The probe can quantitatively determine SO32− with high specificity and sensitivity, fast response (
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- 2019
21. A smart fluorescent probe for discriminative detection of hydrazine and bisulfite from different emission channels
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Juanjuan Wu, Dongdong Su, Zhuo Ye, Jian Pan, and Lintao Zeng
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Detection limit ,High selectivity ,Hydrazine ,Metals and Alloys ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Combinatorial chemistry ,Fluorescence ,Ratiometric fluorescence ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Bisulfite ,chemistry.chemical_compound ,Human health ,chemistry ,Materials Chemistry ,Electrical and Electronic Engineering ,0210 nano-technology ,Instrumentation - Abstract
Hydrazine and bisulfite are poisonous to the environment and human health, hence it is necessary to monitor hydrazine and bisulfite in the environment and living cells. In this study, we reported a smart fluorescent probe (CMCA) for discriminative detection of hydrazine and bisulfite. The single fluorescent probe can respond to hydrazine and bisulfite with two different sets of colorimetric and ratiometric fluorescence signals together with high selectivity, fast response and low detection limit. The discriminative sensing behaviors of the probe for hydrazine and bisulfite were ascribed to different reaction mechanisms, which were confirmed by 1H NMR and HR-MS spectra. The probe has been successfully used to discriminatively determine hydrazine and bisulfite in industrial wastewater with good recoveries. Furthermore, the probe was employed to visualize hydrazine and bisulfite in live cells from different fluorescence channels with good performance. Therefore, this probe has great potential applications for discriminative detection of hydrazine and bisulfite in the environment and living cells.
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- 2018
22. Diversity-Oriented Fluorescence Library Approach (DOFLA) for Discovery of Cell-Permeable Probes for Applications in Live Cell Imaging
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Dongdong Su and Young-Tae Chang
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medicine.anatomical_structure ,Chemistry ,Live cell imaging ,Cell ,medicine ,Fluorescence ,Cell biology - Published
- 2021
23. Trusted Edge Cloud Computing Mechanism Based on FPGA Cluster
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Shen Yanmei, Dongdong Su, Li Rengang, Wang Yanwei, Kan Hongwei, and Liu Wei
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business.industry ,Computer science ,Cloud computing ,Encryption ,law.invention ,law ,Embedded system ,Server ,Internet Protocol ,Data center ,Enhanced Data Rates for GSM Evolution ,business ,Field-programmable gate array ,PCI Express - Abstract
To solve the problem of computing overload in cloud, we intend to design a trusted edge cloud computing model and method based on FPGA (Field Programmable Gate Array) clusters. Firstly, a device, named FPGA Box, with PCIe (Peripheral Component Interconnect Express) power supply capability is used to manage the FPGA accelerator in the model. Besides, the FPGA cluster provide heterogeneous accelerated computing services for the data center through the network. Furthermore, we proposed a trusted edge cloud computing method based on FPGA cluster. On the one hand, a bi-level encryption algorithm based on RSA is proposed to generate an authorized use code, which implied the FPGA accelerator IP (Internet Protocol) address and other information. On the other hand, based on the programmable features of the FPGA accelerators, we set FPGA registers as use status bits, which can control different working status of accelerator. Specifically, when the accelerators has been assigned, we also need upload the deadline of usage to it. Finally, the software activity process of the entire trusted edge cloud system is described in detail, including the process of generating authorized use code. Simulation results show that the edge cloud computing mechanism based on FPGA cluster is proved to be trusted and effective.
- Published
- 2020
24. Energy efficient computing offloading mechanism based on FPGA cluster for edge cloud
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Shen Yanmei, Liu Wei, Dongdong Su, Kan Hongwei, Ou Mingyang, and Wang Yanwei
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User information ,Computer Science::Hardware Architecture ,Task (computing) ,Computer engineering ,Computer science ,business.industry ,Data center ,Cloud computing ,Enhanced Data Rates for GSM Evolution ,Energy consumption ,business ,Field-programmable gate array ,Efficient energy use - Abstract
Towards the computing overload problem in cloud data center, we propose a computing offloading mechanism based on FPGA clusters for edge cloud. Firstly, the FPGA cluster we proposed is decoupled from traditional server, and FPGA BOX is used to power batch FPGA accelerators. The network-based FPGA cluster is deployed at the edge to undertake the computing tasks of the data center. On this basis, we propose an edge cloud network model based on FPGA clusters, and study the energy consumption minimization problem. Secondly, considering the diversity of computing tasks, the MapReduce algorithm based on the numbering mechanism proposed in this paper realizes the classification of computing tasks, and converts the energy consumption minimization problem into the energy consumption minimization problem when the user's computing tasks are determined. Based on the classification results, our improved Hungary algorithm can obtain the minimum energy consumption. Meantime, the computing task results can feedback to corresponding user according to the user information in classification results. Finally, numerical calculation results show that the computing offloading mechanism proposed in this paper shows good performance in terms of energy consumption.
- Published
- 2020
25. A specification that supports FPGA devices on the TensorFlow framework
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Wang Yanwei, Guangkui Huang, Hongbo Zhao, Kan Hongwei, Qianqian Zhao, and Dongdong Su
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Speedup ,Artificial neural network ,Computer science ,business.industry ,Embedded system ,Process (computing) ,Inference ,Original Device ,Field-programmable gate array ,business ,MNIST database - Abstract
With the rise of artificial intelligence and machine learning, many applications and services require FPGA support to speed up the training process and improve efficiency. FPGA has its unique advantages including inference; but now TensorFlow only supports CPU and GPU, TPU, does not support FPGA, cannot use FPGA to accelerate specific models, and cannot play the full role of FPGA in TensorFlow. Based on the above problems, this paper proposes a method for efficiently using FPGA in TensorFlow. This method uses TensorFlow's original device management mechanism, adds an abstract method for FPGA devices under the TensorFlow framework, and writes implementation specifications for FPGA operators. Finally, we used OpenCL to build kernels of FPGA devices, took full advantage of the parallel computing advantages of FPGA devices, and used the CNN LeNet5 model and MNIST dataset to conduct corresponding experiments. The experimental results show that the training accuracy of the two devices is basically the same. This paper provides a feasible solution for TensorFlow to use FPGA devices for neural network calculations.
- Published
- 2020
26. An Optimal Design Method of Conv2d Operator for TensorFlow Based on FPGA Accelerator
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Dongdong Su, Kan Hongwei, Wang Yanwei, Hongbo Zhao, Li Rengang, and Tong Peilin
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Optimal design ,Operator (computer programming) ,Artificial neural network ,Computer science ,Node (networking) ,Central processing unit ,Parallel computing ,General-purpose computing on graphics processing units ,Field-programmable gate array ,MNIST database - Abstract
Currently, TensorFlow architecture only supports CPU and GPU programming, and has not yet formed a unified support standard for FPGAs. To the best of our knowledge, when forward operators in TensorFlow specifies a new device, the backward gradient operator in the same neural network cannot use the same device, which does not comply with rules about node device allocation in TensorFlow. Therefore, we propose an improved algorithm for node device allocation based on placement mechanism and an optimization algorithm for conv2d operator based on OpenCL. The proposed improved algorithm for node device allocation makes forward and backward operators based on FPGA accelerator satisfy the node and device allocation requirements for all TensorFlow operators, and the conv2d operator optimization algorithm based on OpenCL takes full advantage of the parallel computing advantages of FPGA. Finally, this paper uses the CNN LeNet5 model and the MNIST dataset to conduct corresponding experiments. Referring to conv2d operator, based on FPGA accelerator, we implement both the forward and backward operators involved in the first four layers of the model. The experimental results show that the accuracy of the three methods is above 98%. Compared with CPU and GPU, the accuracy difference is only about five thousandths. In addition, in the case of different batch sizes, we tested the runtime of conv2d operator in the first layer of this model. The results show that when the input batch size increased to 10000, the FPGA runs 9 times faster than the CPU. It proved that we proposed an optimization solution for TensorFlow to use FPGA operators for neural network calculations.
- Published
- 2020
27. Target identification of a macrocyclic hexaoxazole G-quadruplex ligand using post-target-binding visualization
- Author
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Young-Tae Chang, Yuki Wakabayashi, Kazuo Nagasawa, Yue Ma, Hiroyuki Seimiya, Sachiko Okabe, Dongdong Su, Mizuho Yasuda, and Masayuki Tera
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Fluorophore ,Macrocyclic Compounds ,Stereochemistry ,Alkyne ,010402 general chemistry ,G-quadruplex ,Ligands ,01 natural sciences ,Catalysis ,03 medical and health sciences ,chemistry.chemical_compound ,Cell Line, Tumor ,Materials Chemistry ,Moiety ,Humans ,Oxazoles ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Binding Sites ,Molecular Structure ,Chemistry ,Ligand ,Metals and Alloys ,General Chemistry ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,G-Quadruplexes ,Ceramics and Composites ,Click chemistry ,Azide ,BODIPY - Abstract
Macrocyclic hexaoxazoles (6OTDs) are G-quadruplex (G4) ligands, and some derivatives, such as L2H2-6OTD (1a) bearing two aminobutyl side chains, show cytotoxicity towards cancer cells. To identify the cellular target of 1a, we employed a post-target-binding strategy utilizing click reaction (Huisgen cyclization) between the azide-conjugated ligand L2H2-6OTD-Az (1b) and the cell-permeable dye CO-1 bearing a strained alkyne moiety and the BODIPY fluorophore under Cu-free conditions. We confirmed that introduction of the small azide group did not alter the physical or biological properties, including anti-cancer activity, of 1a, and we also demonstrated bias-free localization of CO-1. The post-binding visualization strategy suggested that L2H2-6OTD (1a) colocalized with RNA G4 in living cells.
- Published
- 2020
28. A high-performance enzyme-activated near-infrared probe for the sensing and tracking of tumor-related NQO1 in cells and in vivo
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Yong Zhang, Xueqian Chen, Qing Yuan, Yongning Bian, Mingrui Li, Dongdong Su, and Xueyun Gao
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Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2022
29. A portable colorimetric and fluorescent sensor for the fast visual detection of phosgene
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Yongning Bian, Yaling Wang, Houqun Yuan, Guang-Ming Bao, and Dongdong Su
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Process Chemistry and Technology ,General Chemical Engineering - Published
- 2022
30. Effect of electroacupuncture at Zusanli (ST 36) on dorsal root nerve signals in normal rats
- Author
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Dongdong, Su, Lianqi, Geng, Jingsha, Zhang, Yingyuan, Chen, Yangyang, Liu, Lijuan, Yan, and Yi, Guo
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Rats, Sprague-Dawley ,Electroacupuncture ,Animals ,Female ,Rats, Wistar ,Spinal Nerve Roots ,Rats - Abstract
To investigate how electroacupuncture (EA) at Zusanli (ST 36) with varying intensities of stimulation affects dorsal root nerve signals in normal rats.Adult female Wistar rats were examined after drug-induced anesthesia and isolation of the L4 dorsal root associated with the Zusanli (ST 36) acupoint, using bipolar platinum electrodes. We applied EA at Zusanli (ST 36) with a continuous wave waveform for 1 min, and recorded action potentials in the L4 spinal cord dorsal root nerve with a frequency of 5 Hz and various current intensity levels (approximately 1-4 mA). In addition, we tested EA with a frequency of 15 Hz and current intensity of 1-4 mA. The L4 spinal cord dorsal nerve bundle action potentials were measured for 1 min of EA. To analyze the discharge frequency, we used the maximum Lyapunov exponent and Lempel-Ziv (LZ) complexity.At a fixed frequency, with increasing intensity of EA, dorsal root nerve filament discharge frequency revealed an initial increase, followed by a decreasing trend. A stimulation intensity of 3 mA induced a significantly greater discharge frequency, compared with stimulation intensities of 2 and 4 mA. EA stimulation evoked neuroelectric signals with chaotic characteristics. Increased intensity led to an initial increase in LZ complexity which then decreased, with a stimulation strength of 3 mA inducing the highest level of LZ complexity.EA of different intensities can induce nerve action potential encoding with different features.
- Published
- 2020
31. Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes† †Electronic supplementary information (ESI) available: Experimental procedures, characterization data, and additional spectra. See DOI: 10.1039/c8sc03308k
- Author
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Lv, Yun, Dan Cheng, Dongdong Su, Chen, Mei, Yin, Bin-Cheng, Yuan, Lin, and Zhang, Xiao-Bing
- Subjects
Chemistry ,urogenital system ,urologic and male genital diseases ,female genital diseases and pregnancy complications - Abstract
Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice., Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙–), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙– with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙– and NIR-O2˙–) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙– in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙– level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙– was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙– in complex nephrotoxicity disease systems.
- Published
- 2018
32. Highly photostable, lysosome-targeted BODIPYs with green to near-infrared emission for lysosome imaging in living cells
- Author
-
Jian Pan, Dongdong Su, Chao Xiong, Lintao Zeng, and Zhuo Ye
- Subjects
Fluorescence-lifetime imaging microscopy ,010405 organic chemistry ,Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Near-infrared spectroscopy ,High selectivity ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,medicine.anatomical_structure ,Lysosome ,Biophysics ,medicine - Abstract
A series of highly photostable, lysosome-targeted BODIPYs with tunable fluorescence from green to the near-infrared region was developed. The probes were found to show an accurate lysosome-targeting ability with high selectivity and high photostability under continuous excitation irradiation. The results show that these lysosome-targeted BODIPYs offer a simple and convenient tool for fluorescence imaging of lysosomes, which will be valuable for understanding the activity and function of lysosomes in related cells.
- Published
- 2018
33. A Cooperative Method of Task Scheduling based on FPGA Cloud Platform
- Author
-
Liu Wei, Zhang Deshan, Dongdong Su, Li Rengang, Chengqi Wang, and Lin Du
- Subjects
Optimization problem ,Computer science ,business.industry ,Fpga acceleration ,Distributed computing ,020206 networking & telecommunications ,Cloud computing ,02 engineering and technology ,Maximization ,Scheduling (computing) ,Server ,0202 electrical engineering, electronic engineering, information engineering ,Loss minimization ,business ,Field-programmable gate array ,Computer Science::Operating Systems - Abstract
A cloud platform based on FPGA acceleration cards cluster and servers cluster is proposed in this paper. Multi-type boards in the cloud platform could provide multi-type application acceleration services to meet the needs of different tenants. Therefore, we propose to design an optimal task scheduling and service scheme to maximize tenants satisfaction and minimize operators benefits loss. Firstly, the problem of task scheduling is considered as a ‘max-min’ joint optimization problem. Then it is transformed into a bi-level optimization problem with the lower as tenants' satisfaction maximization problem and the upper as FPGA cloud platform operators benefits loss minimization problem. Finally, because the lower problem is convex, the MATSuMoTo algorithm is used to obtain the best task scheduling strategy of the cloud platform.
- Published
- 2019
34. Seeing Elastin: A Near-Infrared Zwitterionic Fluorescent Probe for In Vivo Elastin Imaging
- Author
-
Jong-Jin Kim, Sungjin Park, Chai Lean Teoh, Myung Ae Bae, Renzhe Bi, U. S. Dinish, Michiya Matsusaki, Seong Soon Kim, Young-Tae Chang, Animesh Samanta, Malini Olivo, Marie Piantino, Dongdong Su, and Fiona Louis
- Subjects
0301 basic medicine ,Optical contrast ,biology ,Wavelength range ,Chemistry ,General Chemical Engineering ,Biochemistry (medical) ,Near-infrared spectroscopy ,Photoacoustic imaging in biomedicine ,General Chemistry ,Biochemistry ,Fluorescence ,Imaging modalities ,03 medical and health sciences ,030104 developmental biology ,In vivo ,Materials Chemistry ,biology.protein ,Biophysics ,Environmental Chemistry ,Elastin - Abstract
Summary Elastic fibers are present in a variety of tissues and are responsible for their resilience. Until now, no optical contrast agent in the near-infrared (NIR) wavelength range of 700–900 nm has been reported for the imaging of elastic fibers. Here, we report the discovery of a NIR zwitterionic elastin probe ElaNIR (elastin NIR) through fluorescent-image-based screening. The probe was successfully applied for in vitro , ex vivo , and in vivo imaging by various imaging modalities. Age-related elastin differences shown by in vivo fluorescent and photoacoustic imaging indicated that ElaNIR can be a potentially convenient tool for uncovering changes of elastin in live models.
- Published
- 2018
35. Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes
- Author
-
Bin-Cheng Yin, Yun Lv, Xiao-Bing Zhang, Dongdong Su, Mei Chen, Lin Yuan, and Dan Cheng
- Subjects
chemistry.chemical_classification ,Reactive oxygen species ,010405 organic chemistry ,Chemistry ,Superoxide ,Rational design ,Acute kidney injury ,General Chemistry ,010402 general chemistry ,medicine.disease ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Nephrotoxicity ,chemistry.chemical_compound ,Biochemistry ,medicine ,Mouse Kidney ,Oxidative injury - Abstract
Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙-), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙- with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙- and NIR-O2˙- ) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙- in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙- level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙- was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙- in complex nephrotoxicity disease systems.
- Published
- 2018
36. Detection of analytes in mitochondria without interference from other sites based on an innovative ratiometric fluorophore
- Author
-
Tian-Bing Ren, Dongdong Su, Xiao-Bing Zhang, Xing-Xing Zhang, Qian-Ling Zhang, and Lin Yuan
- Subjects
Analyte ,Fluorophore ,010405 organic chemistry ,General Chemistry ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Chemistry ,chemistry.chemical_compound ,Target site ,chemistry ,Interference (communication) ,Mitochondrial targeting ,Biophysics ,Reaction site - Abstract
A new strategy that integrates the targeting group and response moiety together for the preparation of mitochondrial probe was developed. Bioimaging studies have shown that for the first time, the newly designed probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte., Mitochondria are vital organelles that not only produce cellular energy but also participate in many biological processes. Recently, various fluorescent probes have been developed for mitochondrial imaging. However, due to the lack of suitable dyes or strategies, it is difficult for most reported mitochondrial targeting probes to prove whether the analytes they detected are from mitochondria. In addition, positive charge on mitochondrial probes can seriously affect the mitochondrial environment. To address these issues, we herein put forward a novel strategy for probe design based on a smart NIR dye (HDFL) for mitochondrial targeting detection. Compared to general mitochondrial targeting probes that are modified with a target site and a reaction site, the new strategy is to combine the two sites together for a mitochondrial probe that would provide accurate detection of analytes in mitochondria without interference. As a proof of concept, we synthesized a mitochondrial-targetable probe HDFL-Cys for cysteine. Bioimaging studies have shown that the new type of probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte (cysteine) accompanied by the departure of the targeting group (lipophilic cation moieties). Thus, it can specifically detect the analyte in mitochondria without interference from extra-mitochondrial analytes. We anticipate that the new strategy based on the novel NIR dye HDFL may be a potential platform for developing desirable ratiometric fluorescent probes for mitochondrial imaging.
- Published
- 2018
37. A palette of background-free tame fluorescent probes for intracellular multi-color labelling in live cells
- Author
-
Hee-Sung Park, Kyung Jin Lee, José L. Belmonte-Vázquez, Samira Husen Alamudi, Young-Tae Chang, Jung Yeol Lee, Eduardo Peña-Cabrera, and Dongdong Su
- Subjects
chemistry.chemical_classification ,Bioconjugation ,010405 organic chemistry ,Chemistry ,Biomolecule ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry.chemical_compound ,Labelling ,Biophysics ,Click chemistry ,Azide ,BODIPY ,Intracellular - Abstract
A multi-color labelling technique for visualizing multiple intracellular apparatuses in their native environment using small fluorescent probes remains challenging. This approach requires both orthogonal and biocompatible coupling reactions in heterogeneous biological systems with minimum fluorescence background noise. Here, we present a palette of BODIPY probes containing azide and cyclooctyne moieties for copper-free click chemistry in living cells. The probes, referred to as ‘tame probes’, are highly permeable and specific in nature, leaving no background noise in cells. Such probes, which are rationally designed through optimized lipophilicity, water solubility and charged van der Waals surface area, allow us to demonstrate rapid and efficient concurrent multi-labelling of intracellular target components. We show that these probes are capable of not only labelling organelles and engineered proteins, but also showing the intracellular glycoconjugates’ dynamics, through the use of metabolic oligosaccharide engineering technology in various cell types. The results demonstrated in this study thus provide flexibility for multi-spectral labelling strategies in native systems in a high spatiotemporal manner.
- Published
- 2018
38. Optimal Cooperative Wireless Communication for Mobile User Data Offloading
- Author
-
Hongwu Lv, Haibin Lv, Huiqiang Wang, Dongdong Su, Fumin Xia, Guangsheng Feng, and Yongmin Zhang
- Subjects
Optimization problem ,Mobile data offloading ,General Computer Science ,Distributed database ,Computer science ,business.industry ,General Engineering ,Mobile computing ,020206 networking & telecommunications ,bi-level optimization ,utility function ,02 engineering and technology ,cooperative transmission ,Base station ,user satisfaction ,0202 electrical engineering, electronic engineering, information engineering ,Cellular network ,Wireless ,020201 artificial intelligence & image processing ,General Materials Science ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,business ,lcsh:TK1-9971 ,Computer network - Abstract
We study the mobile data offloading problem in cooperative cellular networks, in which the cellular base station can offload a fraction of data traffic to its cooperative Wi-Fi networks. Both user satisfaction and benefit are important for the cellular network. Under the case that the cellular network cannot provide sufficient transmission resources, part of data traffic can be offloaded to its cooperative Wi-Fi networks to guarantee the user satisfaction, even though the benefit loss of the cellular network will be incurred. Meanwhile, considering the users are price sensitive, the operators, including cellular and Wi-Fi, can issue an appreciate unit price to attract more users and more traffic requirements to increase their total benefit. In this paper, we intend to design an optimal pricing scheme to maximize the user satisfaction and minimize the benefit losses of the operators simultaneously. First, we formulate the mobile data offloading problem as a joint optimization of “min–max” problem. Then, we convert the problem into a bi-level optimization problem, in which the lower level is to maximize the user satisfaction and the upper level is to minimize the benefit losses of the operators. Since the lower-level problem is convex, a centralized scheme based on the dynamic coordinate search-based method is employed to find the optimal solution of the bi-level problem. In addition, a distributed method is proposed to obtain the near-optimal solution quickly, where one-third-party agent is used to adjust the data traffic allocation. Simulation results show that the proposed method achieves the near-optimal solutions with limited iterations.
- Published
- 2018
39. Real-Time In Vivo Hepatotoxicity Monitoring through Chromophore-Conjugated Photon-Upconverting Nanoprobes
- Author
-
Xiaogang Liu, Animesh Samanta, Sungjin Park, Wenxuan Jiang, Juanjuan Peng, Angelo H. All, Xiao Zeng, Daniel Teh Boon Loong, Lu Wang, Nam-Young Kang, Sanyang Han, Dongdong Su, Lin Yuan, and Young-Tae Chang
- Subjects
Modern medicine ,Nanotechnology ,General Medicine ,02 engineering and technology ,General Chemistry ,Conjugated system ,Chromophore ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Fever drug ,chemistry.chemical_compound ,chemistry ,In vivo ,Biophysics ,0210 nano-technology ,Luminescence ,Drug toxicity ,Peroxynitrite - Abstract
Drug toxicity is a long-standing concern of modern medicine. A typical anti-pain/fever drug paracetamol often causes hepatotoxicity due to peroxynitrite ONOO- . Conventional blood tests fail to offer real-time unambiguous visualization of such hepatotoxicity in vivo. Here we report a luminescent approach to evaluate acute hepatotoxicity in vivo by chromophore-conjugated upconversion nanoparticles. Upon injection, these nanoprobes mainly accumulate in the liver and the luminescence of nanoparticles remains suppressed owing to energy transfer to the chromophore. ONOO- can readily bleach the chromophore and thus recover the luminescence, the presence of ONOO- in the liver leads to fast restoring of the near-infrared emission. Taking advantages of the high tissue-penetration capability of near-infrared excitation/emission, these nanoprobes achieve real-time monitoring of hepatotoxicity in living animals, thereby providing a convenient screening strategy for assessing hepatotoxicity of synthetic drugs.
- Published
- 2017
40. A new approach for turn-on fluorescence sensing of l-DOPA
- Author
-
Young-Tae Chang, Jungyeol Lee, Lu Wang, Dongdong Su, and Stuart N. Berry
- Subjects
Chemistry ,Metals and Alloys ,Analytical chemistry ,Fluorescence sensing ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Catalysis ,nervous system diseases ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Turn (biochemistry) ,parasitic diseases ,Materials Chemistry ,Ceramics and Composites ,Biophysics ,0210 nano-technology ,Fluorescence response - Abstract
A novel design strategy for the fluorescence sensing of l-DOPA is reported. Resa-Sulf displays a significant turn-on fluorescence response to l-DOPA due to its reduction properties; this sensing mechanism was fully confirmed by mechanistic studies. Furthermore, Resa-Sulf was successfully utilized to quantitatively detect l-DOPA concentrations from a commercially available source.
- Published
- 2017
41. Motion-induced change in emission (MICE) for developing fluorescent probes
- Author
-
Chai Lean Teoh, Xiaogang Liu, Young-Tae Chang, Lu Wang, and Dongdong Su
- Subjects
Analyte ,Materials science ,Molecular motion ,Nanotechnology ,02 engineering and technology ,General Chemistry ,Molecular rotors ,010402 general chemistry ,021001 nanoscience & nanotechnology ,0210 nano-technology ,01 natural sciences ,Fluorescence ,0104 chemical sciences - Abstract
The need for detecting and labelling environmentally and biologically important analytes has driven considerable research efforts in developing fluorescent probes. During the sensing process, molecular motions (i.e., molecular rotations or vibrations) of a flexible fluorescent probe can be significantly altered by its embedding micro-environment or analyte, thereby leading to substantial changes in readout signals. Motion-induced change in emission (MICE) can be utilized as an effective sensing mechanism. However, in comparison to the well-understood sensing mechanisms, such as photo-induced electron transfer (PET), intramolecular charge transfer (ICT), aggregation-induced emission (AIE) and disaggregation-induced emission (DIE), MICE has not been systematically discussed to date. In this tutorial review, we will summarize the concept and mechanisms of MICE for developing single-molecular fluorescent probes, present unique advantages of MICE based sensors, demonstrate their various applications, and discuss technical challenges in this field. We expect that this review will promote a deeper understanding of MICE and facilitate the development of novel MICE based probes.
- Published
- 2017
42. A unique off-on near-infrared QCy7-derived probe for selective detection and imaging of hydrogen sulfide in cells and in vivo
- Author
-
Dongdong Su, Qian Wu, Dan Cheng, Yun Lv, Xiaojun Ren, and Lin Yuan
- Subjects
Hydrogen sulfide ,Regulator ,Endogeny ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Fluorescence spectroscopy ,Analytical Chemistry ,chemistry.chemical_compound ,Mice ,In vivo ,Materials Testing ,Animals ,Humans ,Hydrogen Sulfide ,Instrumentation ,Spectroscopy ,Fluorescent Dyes ,Mice, Inbred BALB C ,Spectroscopy, Near-Infrared ,Near-infrared spectroscopy ,Hep G2 Cells ,equipment and supplies ,021001 nanoscience & nanotechnology ,Fluorescence ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Molecular Imaging ,chemistry ,Microscopy, Fluorescence ,Biophysics ,Female ,Single-Cell Analysis ,0210 nano-technology ,Selectivity ,HeLa Cells - Abstract
Hydrogen sulfide (H2S) has been found to be an important biological regulator that plays important roles in many physiological and pathological processes. Near-infrared (NIR) fluorescent probes capable of selectively detecting H2S in vivo will be useful tools to understand its mechanisms in biological processes. Herein, we reported an easily synthesized and stimuli-responsive NIR fluorescent probe (QCy7-HS) for selective evaluation of endogenous H2S in the living cells and mice. In response to cellular H2S stimulus, QCy7-HS is converted to QCy7 and shows a unique off-on near-infrared fluorescence signal change. The results of selectivity and kinetic studies indicated that our probe has high H2S binding capacity. Therefore, this probe was used for the fluorescence detection of H2S in cells. Moreover, the probe was applied for study exogenous and endogenous H2S in live mice, indicating that the new probe can be used as an efficient tool on H2S related events in living animals.
- Published
- 2019
43. A Near-Infrared Probe Tracks and Treats Lung Tumor Initiating Cells by Targeting HMOX2
- Author
-
Hyung-Ho Ha, Sungjin Park, Jungyeol Lee, Xiao Liu, Nam-Young Kang, Jong-Jin Kim, Seong Soon Kim, Zhenxun Wang, Lu Wang, Haw-Young Kwon, Yong-An Lee, So Young Choi, Seong Cheol Hong, Animesh Samanta, Myung Ae Bae, Young Hyun Yu, Wai Leong Tam, Jun-Seok Lee, Young-Tae Chang, Junyoung Kim, Bing Lim, Jia Hui Jane Lee, Beomsue Kim, and Dongdong Su
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,Lung Neoplasms ,HMOX2 ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,Metastasis ,Mice ,Colloid and Surface Chemistry ,mental disorders ,medicine ,Animals ,Humans ,Fluorescent Dyes ,Spectroscopy, Near-Infrared ,biology ,Chemistry ,Cancer ,General Chemistry ,medicine.disease ,Ligand (biochemistry) ,Small molecule ,Xenograft Model Antitumor Assays ,nervous system diseases ,0104 chemical sciences ,body regions ,Heme oxygenase ,Survival Rate ,Heme Oxygenase (Decyclizing) ,Cancer research ,biology.protein ,Neoplastic Stem Cells ,Biomarker (medicine) ,human activities ,Preclinical imaging - Abstract
Tumor initiating cells (TIC) are resistant to conventional anticancer therapy and associated with metastasis and relapse in cancer. Although various TIC markers and their antibodies have been proposed, it is limited to the use of antibodies for in vivo imaging or treatment of TIC. In this study, we discovered heme oxygenase 2 (HMOX2) as a novel biomarker for TIC and developed a selective small molecule probe TiNIR (tumor initiating cell probe with near infrared). TiNIR detects and enriches the functionally active TIC in human lung tumors, and through the photoacoustic property, TiNIR also visualizes lung TIC in the patient-derived xenograft (PDX) model. Furthermore, we demonstrate that TiNIR inhibits tumor growth by blocking the function of HMOX2, resulting in significantly increased survival rates of the cancer model mice. The novel therapeutic target HMOX2 and its fluorescent ligand TiNIR will open a new path for the molecular level of lung TIC diagnosis and treatment.
- Published
- 2019
44. An ESIPT-Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells
- Author
-
Jianing Hou, Dan Cheng, Yu-Peng Liu, Tian-Bing Ren, Dongdong Su, and Lin Yuan
- Subjects
Detection limit ,Fluorescence-lifetime imaging microscopy ,Full Paper ,010405 organic chemistry ,General Chemistry ,Full Papers ,010402 general chemistry ,Photochemistry ,Condensation reaction ,01 natural sciences ,Fluorescence ,Rapid detection ,0104 chemical sciences ,Highly sensitive ,lcsh:Chemistry ,chemistry.chemical_compound ,sulfite ,Sulfite ,chemistry ,lcsh:QD1-999 ,imaging studies ,fluorescent probe ,Michael reaction ,fluorescence ,ratiometric probes - Abstract
The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δλ=116 nm), which is beneficial for fluorescence imaging research, and its sulfite‐responsive site is based on a rhodamine‐like structure with the emission peak at 566 nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450 nm as a self‐reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30 s) with a low detection limit (44 nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions.
- Published
- 2019
45. Frontispiece: Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
- Author
-
Audrey Tze Ting Khoo, Jungyeol Lee, Aymeric Silvin, H. Shawn Je, Beomsue Kim, Sungjin Park, Xiaogang Liu, Taejoon Kwon, Masahiro Fukuda, Xiao Liu, Weijie Chi, Dongdong Su, Sejong Choi, Diana S. Y. Wan, Florent Ginhoux, Srikanta Sanu, Young-Tae Chang, and Jin-Soo Kim
- Subjects
Turn (biochemistry) ,medicine.anatomical_structure ,Microglia ,Chemistry ,Biophysics ,medicine ,Substrate (chemistry) ,General Chemistry ,Fluorescence ,Catalysis - Published
- 2019
46. Frontispiz: Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
- Author
-
Beomsue Kim, Masahiro Fukuda, Jung‐Yeol Lee, Dongdong Su, Srikanta Sanu, Aymeric Silvin, Audrey T. T. Khoo, Taejoon Kwon, Xiao Liu, Weijie Chi, Xiaogang Liu, Sejong Choi, Diana S. Y. Wan, Sung‐Jin Park, Jin‐Soo Kim, Florent Ginhoux, H. Shawn Je, and Young‐Tae Chang
- Subjects
General Medicine - Published
- 2019
47. De Novo Design of Chemical Stability Near-Infrared Molecular Probes for High-Fidelity Hepatotoxicity Evaluation In Vivo
- Author
-
Mei Chen, Juanjuan Peng, Lin Yuan, Dongdong Su, Dan Cheng, Xiao-Bing Zhang, Dongjie Liu, and Yun Lv
- Subjects
Fluorescence-lifetime imaging microscopy ,Cell Survival ,Infrared Rays ,010402 general chemistry ,Protective Agents ,01 natural sciences ,Biochemistry ,Catalysis ,Leucyl Aminopeptidase ,Colloid and Surface Chemistry ,In vivo ,Peroxynitrous Acid ,Humans ,Acetaminophen ,Fluorescent Dyes ,Detection limit ,Molecular Structure ,Chemistry ,Near-infrared spectroscopy ,Optical Imaging ,Rational design ,General Chemistry ,Hep G2 Cells ,Fluorescence ,0104 chemical sciences ,Biophysics ,Chemical stability ,Chemical and Drug Induced Liver Injury ,Molecular probe ,Biomarkers - Abstract
Near-infrared (NIR) fluorescence imaging technique is garnering increasing research attention due to various advantages. However, most NIR fluorescent probes still suffer from a false signals problem owing to their instability in real application. Especially in a pathological environment, many NIR probes can be easily destroyed due to the excessive generation of highly reactive species and causing a distorted false signal. Herein, we proposed an approach for developing a new stable NIR dye platform with an optically tunable group to eliminate false signals using the combination of dyes screening and rational design strategy. The conception is validated by the construction of two high-fidelity NIR fluorescent probes (NIR-LAP and NIR-ONOO–) sensing leucine aminopeptidase (LAP) and peroxynitrite (ONOO–), the markers of hepatotoxicity. These probes (NIR-LAP and NIR-ONOO–) were demonstrated to sensitively and accurately monitor LAP and ONOO– (detection limit: 80 mU/L for LAP and 90 nM for ONOO–), thereby allow...
- Published
- 2019
48. Activatable imaging probes for cancer-linked NAD(P)H:quinone oxidoreductase-1 (NQO1): Advances and future prospects
- Author
-
Xueqian Chen, Xueyun Gao, Dongdong Su, and Yong Zhang
- Subjects
Early cancer ,business.industry ,010401 analytical chemistry ,High selectivity ,Cancer ,Computational biology ,medicine.disease ,Quinone oxidoreductase ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Biomarker (cell) ,Medicine ,NAD+ kinase ,business ,Spectroscopy - Abstract
NAD(P)H:quinone oxidoreductase-1 (NQO1) is attractive as a potential cancer biomarker because it is abnormally expressed in many solid tumors and is closely related to a variety of carcinogenic processes. Determining and evaluating the content/activity of NQO1 in tumors is of great significance for early cancer diagnosis and therapeutic effect evaluation. Enzyme-activated imaging probes with tailored properties, high selectivity and sensitivity, and high temporal and spatial resolution have become powerful tools for detecting and monitoring NQO1 activity. In this review, we aim to offer a rigorous but succinct overview of activatable imaging probes by reporting the significant progress made in accurately detecting and imaging NQO1 activity. The design strategies, mechanisms and applications of relevant probes are rationally analyzed and discussed. We also emphasized the potential challenges and prospects of NQO1-activated imaging probes to further advance the development of diagnosis and therapeutic methods for NQO1-related cancers.
- Published
- 2020
49. A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells
- Author
-
Beomsue Kim, Wang Xu, Yong Ni, Lu Wang, Jun Cheng Er, Xian Zeng, Bikram Keshari Agrawalla, Young-Tae Chang, Dongdong Su, Juanjuan Peng, and Lin Yuan
- Subjects
Nanotechnology ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,Adenosine Triphosphate ,Cell Line, Tumor ,Humans ,Binding site ,Fluorescent Dyes ,Binding Sites ,010405 organic chemistry ,Chemistry ,Chemiosmosis ,General Chemistry ,General Medicine ,Fluorescence ,In vitro ,0104 chemical sciences ,Mitochondria ,Microscopy, Fluorescence ,Apoptosis ,Biophysics ,Adenosine triphosphate ,Intracellular - Abstract
Adenosine triphosphate (ATP), commonly produced in mitochondria, is required by almost all the living organisms; thus fluorescent probes for monitoring mitochondrial ATP levels fluctuation are essential and highly desired. Herein, we report a multisite-binding switchable fluorescent probe, ATP-Red 1, which selectively and rapidly responds to intracellular concentrations of ATP. Live-cell imaging indicated that ATP-Red 1 mainly localized to mitochondria with good biocompatibility and membrane penetration. In particular, with the help of ATP-Red 1, we successfully observed not only the decreased mitochondrial ATP levels in the presence of KCN and starvation state, but also the increased mitochondrial ATP levels in the early stage of cell apoptosis. These results indicate that ATP-Red 1 is a useful tool for investigating ATP-relevant biological processes.
- Published
- 2015
50. Development of a Highly Selective, Sensitive, and Fast Response Upconversion Luminescent Platform for Hydrogen Sulfide Detection
- Author
-
Wang Xu, Chai Lean Teoh, Young-Tae Chang, Lu Wang, Juanjuan Peng, Xiaogang Liu, Dongdong Su, Animesh Samanta, Lin Yuan, and Xiao Zeng
- Subjects
Materials science ,Hydrogen sulfide ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,equipment and supplies ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Highly selective ,01 natural sciences ,Photon upconversion ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Upconversion nanoparticles ,chemistry.chemical_compound ,chemistry ,Electrochemistry ,Monitoring methods ,0210 nano-technology ,Luminescence - Abstract
Hydrogen sulfide (H2S) has been recognized as one of most important gaseous signaling molecules mediated by a variety of physiological and pathological processes. Yet, its functions remain largely elusive due to the lack of potent monitoring methods. Hereby this issue is addressed with a powerful new platform—dye-assembled upconversion nanoparticles (UCNPs). A series of chromophores with different absorption bands and fast responses towards H2S is combined with UCNPs and results in a library of H2S sensors with responsive emission signals ranging from the visible to the near-infrared (NIR) region. These nanoprobes demonstrate highly selective and rapid responses to H2S in vitro and in cells. Furthermore, H2S levels in blood can be detected using the developed nanoprobes. Hence the reported H2S sensing platform can serve as a powerful diagnostic tool to research H2S functions and to investigate H2S-related diseases.
- Published
- 2015
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