48 results on '"Tandon, Sudeep"'
Search Results
2. An Insight into Acute Disseminated Encephalomyelitis.
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Nooreen, Summayya F, Wal P, Wal A, Rai AK, and Tandon S
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Background: Acute disseminated encephalomyelitis (ADEM), a neural and immune related state that occur when cerebrospinal system's damaged by extensive swelling. Although manifestation is possible no matter the age, adolescents have a greater probability that adults. The purpose of present manuscript is to provide recent advancement and enhance knowledge of the disease., Method: The literature search on etiology, pathophysiology, diagnosis and treatment was carried out using the online database of Scifinder, Medline, Pubmed and GoogleScholar, Scopus etc. Result: Although the cause of ADEM remains unclear, it is believed to be caused by the inflammation in those with genetic sensitivity to an environmental stimulation. When people have altered levels of awareness or multifocal neurological abnormalities, ADEM is a possibility as a diagnosis. The diagnosis of ADEM is dependent on a combination of clinical, radiologic symptoms and the exclusion of illnesses that mimic ADEM; there is no one test that can establish the diagnosis. The inflammation in a child's brain and spinal cord is treated with medication. Prednisone will occasionally be given to youngsters for a brief amount of time., Conclusion: Most children with ADEM improve with high doses of methylprednisolone. Cyclophosphamide and hypothermia was need to individual. Most investigations show that 50%-75% of individuals completely recover between the first and sixth month of their condition., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
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- 2024
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3. Cu-catalyzed click reaction in synthesis of eugenol derivatives as potent antimalarial agents.
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Jyoti, Bhatt D, Kumar S, Maurya A, Pal A, Darokar MP, Bawankule DU, and Tandon S
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Eugenol( 1 ), a terpenoid found in Ocimum, has various biological activities. The present study aims at extraction, isolation of the plant secondary metabolite eugenol ( 1 ), it's derivatisation and structure identification as bioactive molecules. Synthesis and antiplasmodial activity ( in-vitro and in-vivo ), of a series of fourteen novel eugenol-based 1,2,3-triazole derivatives was done in the present study. Derivatives 5a - 5n showed good antimalarial activity against the strain Plasmodium falciparum NF
54 . Derivative 5 m , IC50 at 2.85 µM was found to be several times better than its precursor 1 (106.82 µM) whereas the derivative 5n showed three fold better activity than compound 1 , in vitro . The structure-activity relationship of the synthesised compounds indicated that the presence of triazole ring in eugenol analogues is responsible for their good activity. Compound 5m , was further evaluated for in-vivo antimalarial activity which showed about 79% parasitemia suppression. It is the first report on antimalarial activity of triazole eugenol derivatives.- Published
- 2024
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4. An Updated Insight into Phytomolecules and Novel Approaches used in the Management of Breast Cancer.
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Nooreen Z, Tandon S, Wal A, and Rai AK
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- Humans, Female, Drug Delivery Systems, Antineoplastic Agents, Phytogenic therapeutic use, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic administration & dosage, Nanoparticles chemistry, Breast Neoplasms drug therapy, Breast Neoplasms pathology, Phytochemicals pharmacology, Phytochemicals therapeutic use, Phytochemicals chemistry
- Abstract
Breast cancer is a widespread condition that kills more women from cancer-related causes than any other type of cancer globally. Women who have estrogen-dependent, initial metastatic breast cancer frequently receive treatment with surgery, radiation therapy, and chemotherapy. They may also get more specialized treatments like tamoxifen or aromatase inhibitors (anastrozole or letrozole). The World Health Organisation reported in 2012 that by 2030, breast cancer will be more common worldwide. There are several phytochemicals, such as isoflavones, coumestans, lignans, and prenylflavonoides. Isoflavones have been shown in studies to prevent the spread of breast cancer and to trigger apoptosis. Targeting BCs in metastatic breast cancer may be made possible by combining well-formulated phytochemicals in nanoparticles or other novel drug delivery agents with currently accepted endocrine and/or conventional chemotherapies. Cell signaling, regulation of cell cycles, oxidative stress action, and inflammation could be positively impacted by phytoconstituents. They have the ability to alter non-coding RNAs, to prevent the proliferation and regeneration of cancer cells. The availability of novel approaches helps in disease targeting, safety, effectiveness and efficacy. The current literature helps to know the available drugs i.e. phytoconstituents or novel drug delivery like nanoparticle, microsphere, micelles, liposomes and neosomes. The literature has been taken from PubMed, Google Scholar, SciFinder, or other internet sites., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
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- 2024
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5. An Insight of Naturally Occurring Phytoconstituents and Novel Approaches Towards the Treatment of Diabetes.
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Nooreen Z, Rai AK, Summayya F, and Tandon S
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- Humans, Hypoglycemic Agents therapeutic use, Hypoglycemic Agents chemistry, Drug Delivery Systems, Diabetes Mellitus drug therapy, Plants, Medicinal chemistry
- Abstract
Background: The rising in diabetes incidents has clearly become one main worldwide health problem. Individuals suffering from diabetes are still more susceptible to many long-term and short-term side effects, which most often cause fatalities. Even though chemically synthesized anti-diabetic entities are capable of helping manage and treat, there has been significant risks related with their prolong and repetitive use. Hence, there is a requirement for safer and novel approaches that might be formed and utilized., Objective: Aim of the present review is to explain the naturally occurring phytochemicals and novel approach as anti-diabetic agents in the treatment of diabetes and its related issues., Method: A survey of Google scholar, Research Gate, Pubmed, Science Direct, NCBI database was carried out conducted to determine a most hopeful phytochemicals and novel drug delivery systems in the management of diabetes., Result: The study stressed the significance of phytomolecules and some novel approaches researched or reported in the literature for the management and cure of diabetes. It is suggested that changes in lifestyle can help patients and like nutritional support, assessment and lifestyle guidance must be individualized based on physical and functional capacity. Further evaluations and improved preventative medicine were the result of improving patient outcomes., Conclusion: Conventional or synthetic drugs provide relief for short time but nanoformulations of phytomolecules offer an improved therapeutic with fewer negative side effects. Herbal medicines are rich in phytoconstituents and possess variety of health benefits. This review is compilation of phytoconstituents and novel drug delivery system of phytomolecules i.e. nanoparticles, niosomes, microsphere, microparticle and others., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
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- 2024
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6. Synthesis of 6-alkoxy and 6-hydroxy-alkyl amine derivatives of braylin as vasorelaxing agents.
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Nainawat KS, Singh S, Agarwal K, Iqbal H, Rani P, Bhatt D, Khan S, Chanda D, Bawankule DU, Tandon S, Khan F, Kumar Gupta A, and Gupta A
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- Animals, Rats, Alcohols, Amines pharmacology, Calcium Channels, L-Type pharmacology, Vasodilation
- Abstract
Braylin (10b) is a 8,8-dimethyl chromenocoumarin present in the plants of the family Rutaceae and Meliaceae and possesses vasorelaxing and anti-inflammatory activities. In this study, six 6-alkoxy (10b, 15-19), and twelve 6-hydroxy-alkyl amine (20a-20l) derivatives of braylin (11 and 12) were synthesized to delineate its structural requirement for vasorelaxing activity. The synthesized compounds were evaluated for vasorelaxation response in preconstricted intact rat Main Mesenteric Artery (MMA). The compounds showed l-type VDCC channel blockade depended and endothelium-independent vasorelaxation within the range of Emax < 50.00-96.70 % at 30 µM. Amongst all, 6-alkoxy derivatives were more active than 6-hydroxy-alkyl amine derivatives. The structural refinements about braylin showed that deletion of its methoxy group or homologation beyond ethoxy group presented deleterious effect on vasorelaxation response of braylin. Interestingly, substituting the ethoxy group in 10b presented the best activity and selectivity towards l-type VDCC channel blockade, a specific target cardiovascular function., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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7. Liquiritigenin, isoliquiritigenin rich extract of glycyrrhiza glabra roots attenuates inflammation in macrophages and collagen-induced arthritis in rats.
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Babu V, Kapkoti DS, Binwal M, Bhakuni RS, Shanker K, Singh M, Tandon S, Mugale MN, Kumar N, and Bawankule DU
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- Rats, Mice, Animals, Inflammation drug therapy, Inflammation metabolism, Plant Extracts therapeutic use, Cytokines metabolism, Macrophages, Arthritis, Experimental drug therapy, Arthritis, Experimental metabolism, Glycyrrhiza metabolism
- Abstract
Liquiritigenin (LTG) and its bioprecursor isoliquiritigenin(ISL), the main bioactives from roots of Glycyrrhiza genus are progressively documented as a potential pharmacological agent for the management of chronic diseases. The aim of this study was to evaluate the pharmacological potential of liquiritigenin, isoliquiritigenin rich extract of Glycyrrhiza glabra roots (IVT-21) against the production of pro-inflammatory cytokines from activated macrophages as well as further validated the efficacy in collagen-induced arthritis model in rats. We also performed the safety profile of IVT-21 using standard in-vitro and in-vivo assays. Results of this study revealed that the treatment of IVT-21 and its major bioactives (LTG, ISL) was able to reduce the production of pro-inflammatory cytokines (TNF-α, IL-6) in LPS-activated primary peritoneal macrophages in a dose-dependent manner compared with vehicle-alone treated cells without any cytotoxic effect on macrophages. In-vivo efficacy profile against collagen-induced arthritis in Rats revealed that oral administration of IVT-21 significantly reduced the arthritis index, arthritis score, inflammatory mediators level in serum. IVT-21 oral treatment is also able to reduce the NFкB-p65 expression as evidence of immunohistochemistry in knee joint tissue and mRNA level of pro-inflammatory cytokines in paw tissue in a dose-dependent manner when compared with vehicle treated rats. Acute oral toxicity profile of IVT-21 demonstrated that it is safe up to 2000 mg/kg body weight in experimental mice. This result suggests the suitability of IVT-21 for further study in the management of arthritis and related complications., (© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
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- 2023
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8. Taxoids-rich extract from Taxus wallichiana alleviates high-fat diet-induced insulin resistance in C57BL/6 mice through inhibition of low-grade inflammation.
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Binwal M, Babu V, Israr KMM, Kashyap PK, Maurya AK, Padalia RC, Tandon S, and Bawankule DU
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- Mice, Animals, Diet, High-Fat, Taxoids therapeutic use, Mice, Inbred C57BL, Inflammation drug therapy, Insulin metabolism, Blood Glucose metabolism, Insulin Resistance physiology, Taxus metabolism
- Abstract
The needle powder of Taxus wallichiana is in use for the management of diabetes and inflammation-related complications in the Indian and Chinese Systems of Traditional Medicine but the lack of proper pharmacological intervention has prompted us to investigate the pharmacological mechanism against inflammation-induced insulin resistance in high-fat diet-fed C57BL/6 mice. Hexane (Tw-H), chloroform (Tw-C), and ethyl acetate (Tw-EA) extracts were prepared from a needle of T. wallichiana and its effect on glucose uptake against TNF-α-induced insulin resistance in skeletal muscle cells was studied. Among all, Tw-EA extract has shown promising glucose uptake potential. Tw-EA treatment is also able to decrease the lipid accumulation in adipocytes. Chemical signature of Tw-EA using HPLC showed the presence of taxoids. Efficacy of taxoids-rich extract from T. wallichiana (Tw-EA) was further validated in in vivo system against high-fat diet (HFD)-induced insulin resistance in C57BL/6 mice. Oral treatment of Tw-EA showed significant reduction in blood glucose, pro-inflammatory cytokine production and body weight gain when compared with vehicle-treated HFD-induced insulin resistance in C57BL/6 mice. Histopathology and immunohistochemistry study in skeletal muscle and adipose tissue revealed that oral treatment of Tw-EA is able to reduce the infiltration of inflammatory cells in skeletal muscles, ameliorate the hypertrophy in adipose tissue and upregulate the GLUT4 protein expression. Treatment with Tw-EA significantly up-regulated mRNA expression of insulin signaling pathway (IRS-1, PI3K, AKT, GLUT 4). This study suggested the beneficial effect of taxoids-rich extract from Taxus wallichiana against the inflammation-associated insulin resistance condition., (© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
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- 2023
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9. Pharmacological and Toxicological Study of Coumarinolignoids from Cleome viscosa in Small Animals for the Management of Rheumatoid Arthritis.
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Babu V, Singh R, Kashyap PK, Washimkar KR, Mugale MN, Tandon S, and Bawankule DU
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- Rats, Animals, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents therapeutic use, Analgesics therapeutic use, Cytokines metabolism, Cleome metabolism, Arthritis, Rheumatoid chemically induced, Arthritis, Rheumatoid drug therapy, Arthritis, Rheumatoid metabolism, Arthritis, Experimental chemically induced, Arthritis, Experimental drug therapy, Arthritis, Experimental metabolism
- Abstract
This study aims to explore the possible pharmacological potential of Cleome viscosa Linn (Cleomaceae), an annual weed, into therapeutic value-added products. In the present study, we have explored the pharmacological and toxicological profile of coumarinolignoids isolated from Cleome viscose for the management of rheumatoid arthritis and related complications in a small animal model. To avoid the biasness during experiments on animals, we have coded the isolated coumarinolignoids as CLIV-92 to perform the experimental pharmacological study. CLIV-92 was orally administrated (30,100, 300 mg/kg) to animal models of collagen-induced arthritis (CIA), carrageenan-induced acute inflammation, thermal and chemical-induced pain, and Brewer's yeast-induced pyrexia. Oral administration of CLIV-92 significantly decreases the arthritis index, arthritis score, and increases the limb withdrawal threshold in the CIA model in experimental rats. The anti-arthritis studies revealed that the anti-inflammatory effect of CLIV-92 was associated with inhibition of the production of inflammatory mediators like TNF- α , IL-6, IL-17A, MMP-1, MMP-9, Nitric oxide, and C-RP in CIA rat's serum, and also reduced the NF к B-p65 expression as evidence of immunohistochemistry in knee joint tissue of CIA rats, in a dose-dependent manner. Further individual experiments related to arthritis-related complications in experimental animals demonstrated the analgesic, anti-inflammatory, and antipyretic potential of CLIV-92 in a dose-dependent manner. Further, an in-vivo acute oral toxicity study concluded that CLIV-92 is safe in experimental animals up to 2,000 mg/kg dose. The results of this study suggested that the oral administration of CLIV-92 may be a therapeutic candidate for further investigation in the management of rheumatoid arthritis and related complications., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)
- Published
- 2023
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10. An efficient process for the extraction of lutein and chemical characterization of other organic volatiles from marigold (Tagetes erecta L.) flower.
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Kumar Kashyap P, Singh S, Kumar Singh M, Gupta A, Tandon S, Shanker K, Kumar Verma R, and Swaroop Verma R
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- Flowers chemistry, Lutein chemistry, Solvents, Calendula, Tagetes chemistry
- Abstract
Marigold (Tagetes erecta L.) petals are the primary industrial source of lutein, which is used as a colouring agent and nutrient supplement to foods. This research extracted marigold petals using different solvents, covering conventional and non-toxic green solvents. The oleoresin, free lutein, and recrystallized lutein yields varied from 8.47-16.67%, 2.56-9.62%, and 1.11-1.61%, respectively. The purity of lutein was achieved up to 92.57% and 97.64% in conventional and newly established green methods, respectively. The present study described an efficient green process to isolate lutein with significantly improved yield (2.56%) and purity (97.33%) over the conventional methods. Based on the results, 2-methyltetrahydrofurancould be a practical green alternative to the traditional toxic solvents for the processing of lutein. Further, the chemical analysis of the essential oil of the residual receptacles obtained after removing petals revealed the presence of important organic volatiles, including piperitone (54.7%) and piperitenone oxide (6.5%), indicating its usefulness for value-addition., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
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- 2022
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11. Linalool reduces the virulence of Pseudomonas syringae pv. tomato DC 3000 by modulating the PsyI/PsyR quorum-sensing system.
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Deepa N, Chauhan S, Kumari P, Rai AK, Tandon S, and Singh A
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- Quorum Sensing genetics, Virulence genetics, Bacterial Proteins metabolism, Acyl-Butyrolactones metabolism, Pseudomonas syringae genetics, Solanum lycopersicum
- Abstract
Quorum sensing modulatory effect of linalool was tested for the first time against Pseudomonas syringae pv. tomato DC 3000, a bacterial plant pathogen responsible for causing huge losses worldwide. DC 3000 extensively regulates its virulence traits through quorum sensing mechanism involving expression of psyI, an acyl-homoserine lactone (AHL) synthase gene and psyR, a luxR-type regulator. In this study, exposure of DC 3000 to 80 ppm linalool concentration resulted in reduced biofilm formation, hampered motility, decreased AHL production, and reduced secretion of plant cell wall-degrading enzymes followed by negligible effect on the bacterial count and its metabolic activity. Extracellular polymeric substances (EPS), which play a crucial role in the development of biofilm and subsequent infection was significantly reduced which was further confirmed by Fourier Transform Infrared Spectroscopy. The qRT PCR analysis of the gene expression analysis of virulence genes (syringafactin production gene syfA, type III secretion system gene hrpA, flagellar genes fleQ and fliC, and coronatine production gene cfl unveiled significant downregulation of the same under Lin 80 ppm concentration. To further confirm the aforementioned possibilities, docking simulations run between PsyR and linalool suggested a strong interaction with the developed protein model. Overall, the treatment of DC 3000 with Lin 80 ppm affected the phenotypic and genotypic expressions associated with quorum sensing, thereby significantly reducing the infection rate as observed in in-vivo plant assay., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare. All co-authors have seen and agree with the contents of the manuscript and there is no financial interest to report. We certify that the submission is original work and is not under review at any other publication., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
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- 2022
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12. Corrigendum: Calliterpenone, a natural plant growth promoter from a medicinal plant Callicarpa macrophylla , sustainably enhances the yield and productivity of crops.
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Pandey P, Pandey SS, Awasthi A, Tripathi A, Singh HP, Singh AK, Tandon S, and Kalra A
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[This corrects the article DOI: 10.3389/fpls.2022.960717.]., (Copyright © 2022 Pandey, Pandey, Awasthi, Tripathi, Singh, Singh, Tandon and Kalra.)
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- 2022
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13. Calliterpenone, a natural plant growth promoter from a medicinal plant Callicarpa macrophylla , sustainably enhances the yield and productivity of crops.
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Pandey P, Pandey SS, Awasthi A, Tripathi A, Singh HP, Singh AK, Tandon S, and Kalra A
- Abstract
The global population is rising at an alarming rate, which is threatening food and nutritional security. Although chemical fertilizers and pesticides are important for achieving food security, their excessive usage critically affects soil health and adds up residues in the food chain. There is an increasing interest in identifying eco-friendly farm inputs that can improve crop productivity through sustainable agricultural practices. One of the most common approaches to reducing chemical inputs in agriculture is the use of plant growth regulators (PGRs). Here, we demonstrate the benefits of a natural and novel plant growth enhancer "calliterpenone," isolated from Callicarpa macrophylla , a medicinal plant, for increasing crop productivity in six crops, viz ., rice, wheat, potato, tomato, chickpea, and onion. Results revealed that the application of calliterpenone (foliar spraying or seed soaking) enhanced the yield of rice (28.89%), onion (20.63%), potato (37.17%), tomato (28.36%), and chickpea (26.08%) at 0.001 mM and of wheat (27.23%) at 0.01 mM concentrations in comparison to control. This enhancement in yield was reflected through improvements in its growth attributes, viz ., spike length, tillers plant
-1 , seeds spike-1 , plant height, and biomass. Furthermore, the exogenous application of calliterpenone could increase the endogenous level of indole-3-acetic acid (IAA) in all tested crops and decrease the content of abscisic acid (ABA) in a few. Trials conducted at farmers' fields showed an overall ~12% increase in rice yield (mean of 11 farmers' fields ranging from 3.48 to 19.63%) and ~10% increase in wheat yield (ranging from 3.91 to 17.51%). The 0.001 mM of calliterpenone was the best effective dose for most crops except wheat, where a concentration of 0.01 mM was found to be the most optimal. This study indicates that calliterpenone is a natural plant growth promoter that can be used in boosting the yields of multiple crops and would be an important input component of organic farming., Competing Interests: The authors declare that the study was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Pandey, Pandey, Awasthi, Tripathi, Singh, Singh, Tandon and Kalra.)- Published
- 2022
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14. Chemical composition and antimicrobial activity of the leaf essential oil of Ravenia spectabilis Engl. (Rutaceae).
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Singh S, Kurmi A, Singh MK, Kashyap PK, Tandon S, Chauhan A, Padalia RC, Saikia D, and Verma RS
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- Anti-Bacterial Agents chemistry, Candida albicans, Gas Chromatography-Mass Spectrometry, Humans, Microbial Sensitivity Tests, Plant Leaves chemistry, Anti-Infective Agents chemistry, Methicillin-Resistant Staphylococcus aureus, Oils, Volatile chemistry, Rutaceae chemistry
- Abstract
Ravenia spectabilis Engl. belongs to the family Rutaceae is known to possess several biologically active phytomolecules. This study was planned to investigate the chemical composition and antimicrobial activity of the leaf essential oil of R. spectabilis . The hydrodistillation of fresh leaves of R. spectabilis gave 0.19 ± 0.02% essential oil. The resulting essential oil was analysed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Altogether, thirty-one constituents forming 97.6 ± 1.72% of the total oil composition were identified. Major components of the oil were sabinene (60.8 ± 0.36%), α -pinene (5.4 ± 0.30%), myrcene (4.8 ± 0.25%), δ -3-carene (4.7 ± 0.62%) and β -pinene (4.3 ± 0.17%). The in-vitro antimicrobial potential of the oil was examined against eight human pathogenic bacterial and fungal strains. The essential oil showed significant activity against Staphylococcus aureus , Staphylococcus epidermidis , methicillin-resistant Staphylococcus aureus , methicillin-resistant Staphylococcus epidermidis , Candida albicans , and Candida kefyr . This is the first report on R. spectabilis leaf essential oil composition and its antimicrobial activity. The essential oil could be a promising natural source of sabinene and antimicrobial for developing new phytotherapeutics.
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- 2022
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15. Towards the development of phytoextract based healthy ageing cognitive booster formulation, explored through Caenorhabditis elegans model.
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Pandey R, Mani D, Shanker K, Bawankule DU, Chanda D, Lal RK, Pal A, Khare P, Kumar N, Tandon S, Saikia D, Gupta AK, Srivastava RK, Kumar S, Suresh R, Singh S, Kalra A, Maurya A, Singh DP, Pandey T, Trivedi S, Smita SS, Pant A, Rathor L, Asthana J, Trivedi M, and Trivedi PK
- Abstract
The positive effect of herbal supplements on aging and age-related disorders has led to the evolution of natural curatives for remedial neurodegenerative diseases in humans. The advancement in aging is exceedingly linked to oxidative stress. Enhanced oxidative stress interrupts health of humans in various ways, necessitating to find stress alleviating herbal resources. Currently, minimal scientifically validated health and cognitive booster resources are available. Therefore, we explored the impact of plant extracts in different combinations on oxidative stress, life span and cognition using the multicellular transgenic humanized C. elegans , and further validated the same in Mus musculus , besides testing their safety and toxicity. In our investigations, the final product-the HACBF (healthy ageing cognitive booster formulation) thus developed was found to reduce major aging biomarkers like lipofuscin, protein carbonyl, lipid levels and enhanced activity of antioxidant enzymes. Further confirmation was done using transgenic worms and RT-PCR. The cognitive boosting activities analyzed in C. elegans and M. musculus model system were found to be at par with donepezil and L-dopa, the two drugs which are commonly used to treat Parkinson's and Alzheimer's diseases. In the transgenic C. elegans model system, the HACBF exhibited reduced aggregation of misfolded disease proteins α-synuclein and increased the health of nicotinic acetylcholine receptor, levels of Acetylcholine and Dopamine contents respectively, the major neurotransmitters responsible for memory, language, learning behavior and movement. Molecular studies clearly indicate that HACBF upregulated major genes responsible for healthy aging and cognitive booster activities in C. elegans and as well as in M. musculus . As such, the present herbal product thus developed may be quite useful for healthy aging and cognitive boosting activities, and more so during this covid-19 pandemic., Supplementary Information: The online version contains supplementary material available at 10.1007/s13237-022-00407-1., Competing Interests: Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© The Author(s) under exclusive licence to Archana Sharma Foundation of Calcutta 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
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- 2022
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16. New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall. ex DC.) Essential Oil and cis-Davanone in Primary Macrophage Cells.
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Singh S, Bhatt D, Singh MK, Sundaresan V, Tandon S, Padalia RC, Bawankule DU, and Verma RS
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- Animals, Cell Survival drug effects, Cells, Cultured, Cytokines metabolism, Female, Lipopolysaccharides antagonists & inhibitors, Lipopolysaccharides pharmacology, Macrophages metabolism, Mice, Oils, Volatile chemistry, Oils, Volatile isolation & purification, Sesquiterpenes chemistry, Sesquiterpenes isolation & purification, Artemisia chemistry, Cytokines antagonists & inhibitors, Macrophages drug effects, Oils, Volatile pharmacology, Sesquiterpenes pharmacology
- Abstract
Artemisia pallens Wall. ex DC., popularly known as davana, has gained considerable attention because of its unique fragrance, high economic value, and pharmacological properties. The compositional complexity of davana essential oil (DO) has been a challenge for quality control. In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques. The analysis led to the identification of ninety-nine compounds. Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %). The study led to identifying several co-eluting novel minor components, which could help determine the authenticity of DO. The rigorous column-chromatography led to the isolation of five compounds. Among these, bicyclogermacrene, trans-ethyl cinnamate, and spathulenol were isolated and characterized by spectroscopic methods for the first time from DO. Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations. The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions., (© 2021 Wiley-VHCA AG, Zurich, Switzerland.)
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- 2021
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17. Cliv-92-Loaded Glycyrrhetinic Acid-Modified Chitosan Nanoparticles for Enhanced Hepatoprotection-Preparation, Characterization, and In Vivo Evaluation.
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Yadav KS, Srivastava N, Rai VK, Ranjana, Tandon S, Mina PR, Chanda D, Kalleti N, Rath SK, Darokar MP, Ajayakumar PV, Shanker K, and Yadav NP
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- Drug Carriers, Particle Size, Solubility, Chitosan, Glycyrrhetinic Acid, Nanoparticles
- Abstract
Cliv-92 is a mixture of three structurally similar coumarinolignoids and a proven hepatoprotective agent. Low aqueous solubility and poor bioavailability are notable hindrances for its further use. Therefore, glycyrrhetinic acid-linked chitosan nanoparticles loaded with Cliv-92 were prepared for active targeting to the liver. The nanoparticles were prepared by the ionic gelation method to avoid the use of toxic solvents/rigorous agitation. The method of preparation was optimized using a central composite design with independent variables, namely polymer: drug ratio (3:1, w/w), crosslinker concentration (0.5%), and stirring speed (750 rpm). The optimized nanoparticles had a mean particle size of 185.17 nm, a polydispersity index of 0.41, a zeta potential of 30.93 mV, and a drug loading of 16.30%. The prepared formulation showed sustained release of approximately 63% of loaded Cliv-92 over 72 h. The nanoparticles were freeze-dried for long-term storage and further characterized. The formulation was found to be biocompatible for parenteral delivery. In vivo imaging study showed that optimized nanoparticles were preferentially accumulated in the liver and successfully targeting the liver. The present study successfully demonstrated the improved pharmacokinetic properties (≈12% relative bioavailability) and efficacy profile (evidenced by in vivo and histopathological studies) of fabricated Cliv-92 nanoparticles., (© 2021. American Association of Pharmaceutical Scientists.)
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- 2021
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18. New chemical constituent from the stem of Cuscuta reflexa Roxb. and its biological activities.
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Nooreen Z, Tandon S, Yadav NP, and Ahmad A
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- Anti-Bacterial Agents pharmacology, Antifungal Agents pharmacology, Carbon-13 Magnetic Resonance Spectroscopy, Microbial Sensitivity Tests, Phytochemicals chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Proton Magnetic Resonance Spectroscopy, Cuscuta chemistry, Phytochemicals pharmacology, Plant Stems chemistry
- Abstract
Chemical investigations on the stem of Cuscuta reflexa Roxb. (Convolvulaceae) led to the isolation of one new compound characterised as 3',4'-dimethoxy-1-phenyl-1α, 2-ethanediol ( 1 ), along with eight known compounds as tridecanyl palmitate, palmitic acid, n-pentatriacontane, n -triacont-21, 27-dien-1-ol, kaempherol, chlorogenic acid, 5,7-dimethoxyapigenin and quercitin. The chemical structures were established with the help of physical, chemical and spectroscopic methods. The antimicrobial potential of the new compound ( 1 ) was evaluated against three bacterial and three fungal pathogenic strain and showed significant activities.
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- 2021
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19. 4-chloro eugenol interacts synergistically with artesunate against drug resistant P. falciparum inducing oxidative stress.
- Author
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Mina PR, Kumar S, Agarwal K, Kumar R, Pal A, Tandon S, Yadav SK, Yadav S, and Darokar MP
- Subjects
- Animals, Calcium metabolism, DNA Damage, Drug Resistance drug effects, Drug Synergism, Lipid Peroxidation drug effects, Malaria, Falciparum drug therapy, Malaria, Falciparum parasitology, Membrane Potential, Mitochondrial drug effects, Mice, Protein Carbonylation drug effects, Reactive Nitrogen Species metabolism, Reactive Oxygen Species metabolism, Antimalarials pharmacology, Artesunate pharmacology, Eugenol pharmacology, Oxidative Stress drug effects, Plasmodium falciparum drug effects
- Abstract
4-chloro eugenol (4CE), a semisynthetic analog of phytomolecule eugenol exhibited potent antiplasmodial activity with IC
50 in the range of 1.5-5 μM against sensitive as well as drug resistant strain of P. falciparum. This analog also showed synergy with a clinically used antimalarial drug artesunate and was able to curtail the IC50 of artesunate up to 4-5 folds. Although, 4CE did not show any effect on heme polymerization, the most common drug target in the malaria parasite, it could increase the level of reactive oxygen species (ROS) and reactive nitrogen species (RNS) alone as well as in combination with artesunate. Further, 4CE induced oxidative stress was observed to affect the macromolecules in terms of DNA damage, protein carbonylation and lipid peroxidation. At the physiological level, cellular organelles like mitochondria and endoplasmic reticulum were observed to be get affected by 4CE in terms of membrane depolarization and calcium ion leakage respectively. These observations could be validated by expression analysis of oxidative stress responsive genes and proteins. Further, in in vivo assay, 4CE showed significant chemo-suppression of parasitemia as well as an increase in mean survival time in the murine malaria model. Interestingly, in combination with artesunate, 4CE showed higher chemo-suppression as well as enhanced mean survival time at a much lower concentrations of both the partners as compared to an individual dose of artesunate and 4CE. A combination of 4CE and artesunate was also observed to attenuate cerebral malaria pathogenesis., (Copyright © 2021 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.)- Published
- 2021
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20. Chemical composition and antimicrobial activity of the essential oil of senescent leaves of guava ( Psidium guajava L.).
- Author
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Chaturvedi T, Singh S, Nishad I, Kumar A, Tiwari N, Tandon S, Saikia D, and Verma RS
- Subjects
- Bacteria drug effects, Fungi drug effects, Gas Chromatography-Mass Spectrometry, Humans, Microbial Sensitivity Tests, Anti-Infective Agents pharmacology, Oils, Volatile chemistry, Oils, Volatile pharmacology, Plant Leaves chemistry, Psidium chemistry
- Abstract
The aim of the study was to investigate the chemical composition and antimicrobial activity of the essential oil of the senescent leaves of Psidium guajava L. (Myrtaceae). The hydrodistilled essential oil of the leaves was analysed by GC-FID and GC-MS. The antimicrobial activity of the oil was assessed against human and plant pathogenic microorganisms by disc diffusion, microdilution broth and poison food techniques. A total of forty-six constituents, representing 90.9% of the total oil composition were identified. Major constituents of the oil were limonene (29.1%), ( E )-caryophyllene (15.7%), caryophyllene oxide (8.8%), caryophylla-4(12),8(13)-dien-5-ol (6.5%), ( E )-nerolidol (4.0%), α -cadinol (3.4%), and muurola-4,10(14)-dien-1- β -ol (2.5%). The chemical composition of the examined essential oil was quite different from those reported earlier. The essential oil showed significant inhibition of human pathogenic bacteria (MIC: 0.065-0.261 mg/mL) and plant pathogenic fungi, namely Curvularia lunata (86.02%) and Fusarium chlamydosporum (82.80%).
- Published
- 2021
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21. 4-Chlorothymol Exerts Antiplasmodial Activity Impeding Redox Defense System in Plasmodium falciparum .
- Author
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Kumar S, Mina PR, Kumar R, Pal A, Ahmad A, Tandon S, and Darokar MP
- Abstract
Malaria remains one of the major health concerns due to the resistance of Plasmodium species toward the existing drugs warranting an urgent need for new antimalarials. Thymol derivatives were known to exhibit enhanced antimicrobial activities; however, no reports were found against Plasmodium spp. In the present study, the antiplasmodial activity of thymol derivatives was evaluated against chloroquine-sensitive (NF-54) and -resistant (K1) strains of Plasmodium falciparum . Among the thymol derivatives tested, 4-chlorothymol showed potential activity against sensitive and resistant strains of P. falciparum . 4-Chlorothymol was found to increase the reactive oxygen species and reactive nitrogen species level. Furthermore, 4-chlorothymol could perturb the redox balance by modulating the enzyme activity of GST and GR. 4-Chlorothymol also showed synergy with chloroquine against chloroquine-resistant P. falciparum . 4-Chlorothymol was found to significantly suppress the parasitemia and increase the mean survival time in in vivo assays. Interestingly, in in vivo assay, 4-chlorothymol in combination with chloroquine showed higher chemosuppression as well as enhanced mean survival time at a much lower concentration as compared to individual doses of chloroquine and 4-chlorothymol. These observations clearly indicate the potential use of 4-chlorothymol as an antimalarial agent, which may also be effective in combination with the existing antiplasmodial drugs against chloroquine-resistant P. falciparum infection. In vitro cytotoxicity/hemolytic assay evidently suggests that 4-chlorothymol is safe for further exploration of its therapeutic properties., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Kumar, Mina, Kumar, Pal, Ahmad, Tandon and Darokar.)
- Published
- 2021
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22. Chemical composition, bactericidal kinetics, mechanism of action, and anti-inflammatory activity of Isodon melissoides (Benth.) H. Hara essential oil.
- Author
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Kumar A, Singh S, Kumar A, Bawankule DU, Tandon S, Singh AK, Verma RS, and Saikia D
- Subjects
- Bacteria drug effects, Cymenes pharmacology, DNA metabolism, Gas Chromatography-Mass Spectrometry, HaCaT Cells, Humans, Kinetics, Microbial Sensitivity Tests, Plant Extracts pharmacology, RNA metabolism, Thymol pharmacology, Anti-Bacterial Agents pharmacology, Anti-Inflammatory Agents pharmacology, Isodon chemistry, Oils, Volatile chemistry, Oils, Volatile pharmacology
- Abstract
Present study was aimed to investigate the antibacterial activity, bactericidal mechanism of action, killing kinetics and anti-inflammatory activity of Isodon melissoides (Benth.) H. Hara essential oil. The gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of carvacrol (45.4%), p -cymene (11.6%) and thymol (11.3%) as major constituents of the oil. The oil displayed broad spectrum significant antibacterial activity (MIC: 0.13-8.33 ppm; MBC: 0.13->33.34 ppm) against test strains. The oil exhibited a time and dose-dependent bactericidal effect. The oil disrupted the cell membrane by changing the cell membrane permeability. The essential oil significantly decreased the overproduction of proinflammatory cytokines in LPS-induced inflammation in HaCaT cells without any cytotoxic effect. I. melissoides essential oil can be a promising alternative antimicrobial agent for the control of methicillin resistant staphylococci and other pathogenic bacteria tested, and also useful for the topical anti-inflammatory properties.
- Published
- 2021
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23. Anti-psoriatic effect of Lavandula angustifolia essential oil and its major components linalool and linalyl acetate.
- Author
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Rai VK, Sinha P, Yadav KS, Shukla A, Saxena A, Bawankule DU, Tandon S, Khan F, Chanotiya CS, and Yadav NP
- Subjects
- Acyclic Monoterpenes administration & dosage, Acyclic Monoterpenes isolation & purification, Administration, Cutaneous, Animals, Cytokines metabolism, Dermatologic Agents administration & dosage, Dermatologic Agents isolation & purification, Disease Models, Animal, Female, Imiquimod, Inflammation Mediators metabolism, Mice, Inbred BALB C, Monoterpenes administration & dosage, Monoterpenes isolation & purification, Oils, Volatile administration & dosage, Oils, Volatile isolation & purification, Plant Oils administration & dosage, Plant Oils isolation & purification, Psoriasis chemically induced, Psoriasis metabolism, Psoriasis pathology, Rabbits, Signal Transduction, Skin metabolism, Skin pathology, Acyclic Monoterpenes pharmacology, Dermatologic Agents pharmacology, Lavandula chemistry, Monoterpenes pharmacology, Oils, Volatile pharmacology, Plant Oils pharmacology, Psoriasis prevention & control, Skin drug effects
- Abstract
Ethno-Pharmacological Relevance: Lavender oil (LO) is an aromatic/essential oil extracted from Lavandula angustifolia and traditionally used as an aromatherapy massage oil due to its anti-inflammatory and wound healing property and also for providing the relief in other skin conditions such as psoriasis, dermatitis and eczema. However, LO has not been evaluated scientifically for psoriasis like skin inflammation., Aim of the Study: This study was aimed to investigate the LO and its major components linalool (L) and linalyl acetate (LA) against psoriasis like skin inflammation., Materials and Methods: Anti-psoriatic activity was done using Imiquimod (IMQ) induced psoriasis like skin inflammation in BALB/c mice. Assessment of anti-psoriatic effect of LO, L and LA was done on the basis of change in ear thickness, psoriasis area severity index (PASI) scoring at alternative day, CosCam scoring using skin analyzer equipped with SkinSys software, biochemical, immunohistochemical and histological investigations. Level of effectiveness against psoriasis was investigated by percent reduction in PASI scores, CosCam scores and level of Th-1 and Th-17 cell expressing cytokines, as compared to the diseased mice., Results: Topical application of LO 10% showed 73.67% recovery in PASI and 87% in Th-17 cell-specific cytokines towards normal as compared to disease group. L and LA were identified as the major components of LO and favoured ligands for selected psoriasis targets. At 2% topical dose, L and LA showed 64% and 47.61% recovery in PASI scores, respectively. Both, L and LA showed significant recovery in Th-1 specific TNF-α and IL-1β however, only L showed significant recovery of Th-17 cytokines (IL-17 and IL-22). In contrast to LA (which restored granulosis), L restored epidermal hyperplasia and parakeratosis toward the normal condition. On the other hand, L also reduced the expression of NF-κβ, ccr6 and IL-17, while LA reduced the expression of NF-κβ only. At 10% topical dose, LO was observed to be slight irritant while at 2% topical dose, L and LA were found non-irritant to the skin., Conclusion: This study proves the effectiveness of LO and its major phytoconstituents linalool and linalyl acetate against IMQ induced psoriasis like skin inflammation and provides the scientific evidence for topical use of lavender oil., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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24. Silymarin, a polyphenolic flavonoid impede Plasmodium falciparum growth through interaction with heme.
- Author
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Mina PR, Kumar Y, Verma AK, Khan F, Tandon S, Pal A, and Darokar MP
- Subjects
- Animals, Antioxidants pharmacology, Apoptosis drug effects, Hemeproteins antagonists & inhibitors, Inhibitory Concentration 50, Plasmodium falciparum growth & development, Silymarin chemistry, Silymarin metabolism, Flavonoids pharmacology, Heme metabolism, Plasmodium falciparum drug effects, Silymarin pharmacology
- Abstract
A polyphenolic flavonoid, Silymarin isolated from Silybum marianum is widely known for its hepatoprotective action. In the present study anti-plasmodial activity of Silymarin has been demonstrated for the first time having IC
50 of 14 ± 0.33 μM against the NF-54 strain of P. falciparum with high selectivity index (>100). The parasitostatic action is exerted through inhibition of β-hematin/hemozoin formation which is due to the interaction ( Kd = 3.63 ± 0.9µM) of silymarin with free heme in a Stoichiometry of 1:1 Silymarin: heme complex resulting into heme-induced membrane damage in the parasite. Silymarin could hinder the glutathione and hydrogen peroxide-induced heme detoxification. Silymarin also induces apoptosis in the parasite through the elevation of caspase-3 level in a dose-dependent manner. Results from the docking studies suggest that Silymarin interacts with heme.- Published
- 2020
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25. Brevifoliol ester induces apoptosis in prostate cancer cells by activation of caspase pathway.
- Author
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Bhukya B, Fatima K, Nagar A, Lakshmi V, Dubey P, Kumar S, Kumar Y, Luqman S, Chanda D, Tandon S, Shanker K, Khan F, and Negi AS
- Subjects
- Acetic Acid chemistry, Animals, Antineoplastic Agents pharmacology, Apoptosis drug effects, Benzoic Acid chemistry, Drug Screening Assays, Antitumor, Esterification, Humans, Male, Mice, Molecular Docking Simulation, Neoplasms, Experimental drug therapy, PC-3 Cells, Plant Extracts pharmacology, Taxoids pharmacology, Taxus chemistry, Antineoplastic Agents chemistry, Esters chemistry, Plant Extracts chemistry, Prostatic Neoplasms drug therapy, Taxoids chemistry
- Abstract
Prostate cancer is fourth most abundant cancer type around the globe. Brevifoliol, a rearranged taxoid from Taxus walllichiana needles has been derivatized as C5 esters using Steglich esterification reaction. Seventeen diverse analogues were evaluated against a panel of human cancer cell lines by MTT assay. Among these, two of the semi-synthetic analogues, that is, 13 and 16 exhibited potent cytotoxicity, selectively against PC-3, prostate cancer cell lines. In cell cycle analysis, analogue 13 induced S and G2/M phase arrest and induced apoptosis by activating caspase-3. Compound 13 showed moderate efficacy in in-vivo Ehrlich ascites carcinoma in Swiss albino mice. Further, compound 13 was found to be safe in Swiss albino mice up to 1,000 mg/kg dose in acute oral toxicity. Brevifoliol ester 13 may further be optimized for better efficacy., (© 2019 John Wiley & Sons A/S.)
- Published
- 2020
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26. Synthesis and evaluation of substituted 8,8-dimethyl-8H-pyrano[2,3-f]chromen-2-one derivatives as vasorelaxing agents.
- Author
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Singh S, Agarwal K, Iqbal H, Yadav P, Yadav D, Chanda D, Tandon S, Khan F, Gupta AK, and Gupta A
- Subjects
- Humans, Structure-Activity Relationship, Drug Discovery methods, Vasodilation drug effects
- Abstract
A series of substituted 8,8-dimethyl-8H-pyrano[2,3-f]chromen-2-ones (chromeno-coumarin hybrids) was synthesized from scopoletin (11) as vasorelaxing agents. The synthesized compounds 21a-f, 22, 23a-e and scopoletin (11) were evaluated for vasorelaxation in endothelium intact rat main mesenteric artery (MMA). Compounds 11, 21a, 21c-f and 22 showed significant vasorelaxation in precontracted MMA within the range of EC
50 value 1.58-5.02 µM. These derivatives presented 29.40-70.89 fold increased sensitivity for experimental tissue compared to scopoletin (11), the parent molecule. Among others, 22 was found to be the most active compound which had EC50 1.58 µM with 70.89 fold increased sensitivity. The mechanistic evaluation of 22 showed that it exerted vasorelaxation through Ca2+ -activated K+ (BKca) channel and the effect was endothelium-independent., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2020
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27. Synthesis of eugenol derivatives and its anti-inflammatory activity against skin inflammation.
- Author
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Maurya AK, Agarwal K, Gupta AC, Saxena A, Nooreen Z, Tandon S, Ahmad A, and Bawankule DU
- Subjects
- Animals, Anti-Inflammatory Agents chemical synthesis, Cytokines antagonists & inhibitors, Eugenol analogs & derivatives, Eugenol pharmacology, Macrophages drug effects, Macrophages pathology, Phytochemicals pharmacology, Anti-Inflammatory Agents pharmacology, Dermatitis drug therapy, Eugenol chemical synthesis
- Abstract
Eugenol is a phytochemical present in aromatic plants has generated considerable interest in the pharmaceutical industries mainly in cosmetics. A series of eugenol esters (ST1-ST7) and chloro eugenol ( ST8 ) have been synthesized. The structures of newly synthesized compounds were confirmed by
1 H and13 C NMR and mass spectrometry. In an effort to evaluate the pharmacological activity of eugenol derivatives, we explored its anti-inflammatory potential against skin inflammation using in-vitro and in-vivo bioassay. Synthesized derivatives significantly inhibited the production of pro-inflammatory cytokines against LPS-induced inflammation in macrophages. Among all derivatives, ST8 [Chloroeugenol (6-chloro, 2-methoxy-4-(prop-2-en-1-yl)-phenol)] exhibited most potent anti-inflammatory activity without any cytotoxic effect. We have further evaluated the efficacy and safety in in-vivo condition. ST8 exhibited significant anti-inflammatory activity against TPA-induced skin inflammation without any skin irritation effect on experimental animals. These findings suggested that ST8 may be a useful therapeutic candidate for the treatment of skin inflammation.- Published
- 2020
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28. Application of essential oils as a natural and alternate method for inhibiting and inducing the sprouting of potato tubers.
- Author
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Shukla S, Pandey SS, Chandra M, Pandey A, Bharti N, Barnawal D, Chanotiya CS, Tandon S, Darokar MP, and Kalra A
- Subjects
- Clove Oil pharmacology, Cymbopogon chemistry, Gene Expression Regulation, Plant drug effects, Meristem drug effects, Plant Tubers drug effects, Plant Tubers growth & development, Plant Tubers metabolism, Oils, Volatile pharmacology, Solanum tuberosum drug effects, Solanum tuberosum physiology
- Abstract
Use of harmful chemicals and expensive maintenance of cold-storage conditions for controlling sprouting are among the major problems in potato storage. Here, 20 essential oils (EOs) were tested for their sprouting-inhibiting and sprouting-inducing activities. Overall, treatments of lemon grass (LG) and clove (CL) oils could induce sprouting whereas palmarosa (PR) and ajwain (AZ) oils could inhibit sprouting of potato tubers at normal-room-temperature (25 ± 2 °C) storage. Selected-EOs treatments affected sprouting by modulation of accumulation of reducing sugars, ethylene, and expression of genes involved in tuber-sprouting such as ARF, ARP, AIP and ERF. Surprisingly, 7-days AZ-treatments could inhibit sprouting for 30-days which was mediated via damaging apical meristem. However, LG- and CL-treated tubers could produce enhanced potato yield as well. Present work clearly demonstrates that selected-EOs can be used as a promising eco-friendly approach for inducing/inhibiting sprouting of potato tubers during potato storage and those enhancing sprouting can be used for enhancing productivity., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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29. New chemical constituents from the fruits of Zanthoxylum armatum and its in vitro anti-inflammatory profile.
- Author
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Nooreen Z, Kumar A, Bawankule DU, Tandon S, Ali M, Xuan TD, and Ahmad A
- Subjects
- Animals, Anti-Inflammatory Agents isolation & purification, Cytokines metabolism, Female, India, Mice, Mice, Inbred BALB C, Molecular Structure, Phytochemicals isolation & purification, Phytochemicals pharmacology, Anti-Inflammatory Agents pharmacology, Fruit chemistry, Macrophages, Peritoneal drug effects, Zanthoxylum chemistry
- Abstract
Chemical investigations on the fruits of Zanthoxylum armatum Roxb. (Rutaceae) led to the isolation of two new constituents characterised as 2α-methyl-2β-ethylene-3β-isopropyl-cyclohexan-1β, 3α-diol (1) and phenol-O-β-D-arabinopyranosyl-4'-(3″, 7″, 11″, 15″-tetramethyl)-hexadecan-1″-oate (2) along with known compounds m-methoxy palmityloxy benzene (3), acetyl phenyl acetate (4), linoleiyl-O-α-D-xylopyranoside (5), m-hydroxyphenoxy benzene (6) and palmitic acid (7). The chemical structures were established with the help of physical, chemical and spectroscopic methods. The anti-inflammatory potential of isolated compounds 1 and 2 was evaluated using in vitro target-based anti-inflammatory activity in LPS-stimulated primary peritoneal macrophages isolated from mice. Production of pro-inflammatory cytokines (TNF-α and IL-6) was significantly inhibited by the treatment of isolated compounds 1 and 2 in a dose-dependent manner.
- Published
- 2019
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30. Standardization and xanthine oxidase inhibitory potential of Zanthoxylum armatum fruits.
- Author
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Ranjana, Nooreen Z, Bushra U, Jyotshna, Bawankule DU, Shanker K, Ahmad A, and Tandon S
- Subjects
- Fruit, Gout Suppressants pharmacology, Plant Extracts pharmacology, Xanthine Oxidase antagonists & inhibitors, Zanthoxylum
- Abstract
Ethnopharmacological Relevance: Tejovati (Zanthoxylum armatum DC; Family- Rutaceae) popularly known as toothache tree is widely distributed in sub-tropical Himalaya region. Traditionally, The Southeast Asian population of Indo-Nepal origin uses it to treat asthma, gout, pain, and inflammation. The Ayurvedic action of the plant includes the balancing of Vata-Kapha in the body. Which lead to various ailments related to the circulation of blood and water, digestion, immunity, and skin. Therefore, in-vitro xanthine oxidase (XO) inhibition potential of the extract could be worth to explore prospect in the prevention/treatment of gouty affections of the joints and other diseases., Aim of Study: Anti-inflammatory and antioxidant potential of Z. armatum fruit (ZAF) has been reported. To date, no scientific study to validate the claim for gout treatment/management has been attempted so far. The present study deals with the xanthine oxidase inhibitory potential of a various extract of ZAF and marker-based high-performance liquid chromatography (HPLC) standardization of most active fraction., Materials and Methods: Liquid-liquid partioning of crude methanol extract of the ZAF followed by repeated column chromatography of most active fraction has resulted in the isolation of seven compounds. Five distinct groups of compounds were isolated, purified, and identified. We have investigated the therapeutic action of ZAF in the management of gout through in-vitro assay of XO, a key enzyme involved in gout pathogenesis., Results: Phytochemical investigation of ZAF has resulted in the isolation of seven compounds of diverse nature. It is noteworthy to mention that out of seven, five compounds have shown the xanthine oxidase inhibitory action. The ethyl acetate fraction was most potent to inhibit XO. The XO inhibitory activity (IC
50 values) of isolated marker chemical was ranging from 5.62 to 41.21 µM. Three compounds viz. acetyl phenyl acetate (ZA-2), prudomestin (ZA-6), and tambulin (ZA-7) showed the most potent XO inhibitory activity (IC50 ≈ 6 µM) comparable with a positive control (Allopurinol, IC50 , 3.38 µM). This is the first validated HPLC-PDA method for simultaneous analysis and accurate quantification of seven compounds (phenolic acid, acetyl phenyl acetate, xylopyranoside, diphenyl ether and three flavones) in ZAF as well as their distribution in other tissues of the plant., Conclusion: Most potent three chemicals (ZA-2, 6 and 7) could be considered as bioactive to ensure the robust quality of the enriched fraction of ZAF with defined XO inhibition potential. Therefore, either single purified component or their enriched fraction could be a better choice for the management of gout than the crude extract of ZAF. Developed HPLC method is suitable for quality assurance analysis and process control of ZAF derived product intended for gout management. XO inhibitory potential exhibited by the characterized compounds validate the traditional use of this ZAF for the treatment of gout. Further, a detailed study is required to assess the effect of ZAF chemicals on serum uric acid and mechanism of XO inhibition., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2019
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31. Essential oil from waste leaves of Curcuma longa L. alleviates skin inflammation.
- Author
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Kumar A, Agarwal K, Singh M, Saxena A, Yadav P, Maurya AK, Yadav A, Tandon S, Chanda D, and Bawankule DU
- Subjects
- Animals, Cell Survival drug effects, Cells, Cultured, Female, Gas Chromatography-Mass Spectrometry, Humans, Mice, Oils, Volatile pharmacology, Rabbits, Tetradecanoylphorbol Acetate, Curcuma chemistry, Dermatitis drug therapy, Oils, Volatile therapeutic use, Plant Leaves chemistry
- Abstract
Background: Curcuma longa L. is an important industrial crop used by medicinal and cosmetic industries in the world. Its leaves are a waste material after harvesting rhizomes. The aim of the study was to evaluate the chemical and pharmacological profile of essential oil from waste leaves of Curcuma longa (EOCl) against skin inflammation., Methods: EOCl was subjected to gas chromatography (GC) analysis for identification of essential oil constituents and its anti-inflammatory evaluation through in vitro and in vivo models., Results: Chemical fingerprinting using GC and GC-MS analysis of EOCl revealed the presence of 11 compounds, representing 90.29% of the oil, in which terpinolene (52.88%) and α-phellandrene (21.13%) are the major components. In the in vitro testing EOCl inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) in lipopolysaccharide (LPS) and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in the human keratinocyte cell line (HaCaT). Topical application of EOCl produced anti-inflammatory effects by reducing ear thickness, ear weight and ameliorating the level of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) at protein and mRNA levels as well as regulating the overproduction of oxidative markers and restoring the histopathological damage in a TPA-induced mouse model of inflammation., Conclusion: These findings of topical anti-inflammatory properties of EOCl provide a scientific basis for medicinal use of this plant material against inflammatory disorders.
- Published
- 2018
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32. AromaDb: A Database of Medicinal and Aromatic Plant's Aroma Molecules With Phytochemistry and Therapeutic Potentials.
- Author
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Kumar Y, Prakash O, Tripathi H, Tandon S, Gupta MM, Rahman LU, Lal RK, Semwal M, Darokar MP, and Khan F
- Abstract
In traditional, herbal medicine, and aromatherapy, use of essential oils and their aroma compounds have been known since long, for the management of various human diseases. The essential oil is a mixture of highly complex, naturally occurring volatile aroma compounds synthesized by medicinal and aromatic plants as secondary metabolites. Essential oils widely used in pharmaceutical, cosmetic, sanitary, food industry and agriculture for their antibacterial, antiviral, antifungal, antiparasitic, insecticidal, anticancer, neuroprotective, psychophysiological, and anti-aging activities. Moreover, volatile aroma compounds comprise a chemically diverse class of low molecular weight organic compounds with significant vapor pressure. However, aroma compounds produced by plants, mainly attract pollinators, seed dispersers and provide defense against pests or pathogens. However, in humans, about 300 active olfactory receptor genes are involved to detect thousands of different aroma compounds and modulates expression of different metabolic genes regulating human psychophysiological activity, brain function, pharmacological signaling, and therapeutic potential. Keeping in mind this importance, present database, namely, AromaDb (http://bioinfo.cimap.res.in/aromadb/) covers information of plant varieties/chemotypes, essential oils, chemical constituents, GC-MS profile, yield variations due to agro-morphological parameters, trade data, aroma compounds, fragrance type, and bioactivity details. The database includes 1,321 aroma chemical structures, bioactivities of essential oil/aroma compounds, 357 fragrance type, 166 commercially used plants, and their high yielding 148 varieties/chemotypes. Also includes calculated cheminformatics properties related to identification, physico-chemical properties, pharmacokinetics, toxicological, and ecological information. Also comprises interacted human genes affecting various diseases related cell signaling pathways correlating the use of aromatherapy. This database could be a useful resource to the plant's growers/producers, an aroma/fragrance industrialist, health professionals, and researchers exploring the potential of essential oils and aroma compounds in the development of novel formulations against human diseases.
- Published
- 2018
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33. Anti-biofilm and anti-virulence potential of 3,7-dimethyloct-6-enal derived from Citrus hystrix against bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae.
- Author
-
Singh A, Gupta R, Tandon S, Prateeksha, and Pandey R
- Subjects
- Acyclic Monoterpenes, Gene Expression Regulation, Bacterial drug effects, Movement drug effects, Plant Diseases microbiology, Plant Leaves microbiology, Virulence Factors genetics, Xanthomonas genetics, Xanthomonas pathogenicity, Aldehydes pharmacology, Biofilms growth & development, Citrus chemistry, Monoterpenes pharmacology, Oils, Volatile pharmacology, Oryza microbiology, Plant Oils pharmacology, Xanthomonas drug effects
- Abstract
The present investigation for the first time explains the anti biofilm and anti virulence potential of Kaffir lime oil (KLO) and its major constituent, Citronellal (3,7-dimethyloct-6-enal) against Xanthomonas oryzae pv. oryzae, causal organism of bacterial blight disease of rice. KLO at 500 ppm showed potential activity against X. oryzae pv. oryzae. Among the major components identified, citronellal (CIT) at 75 μM concentration was found to significantly inhibit biofilm along with the swimming and swarming potential of X. oryzae pv. oryzae. In contrary, CIT did not affect the metabolic status and growth kinetics of the bacterial cells. Gene expression analysis showed down regulation in motA, cheD, cheY, flgF, gumC, xylanase, endogluconase, cellulose, cellobiosidase, virulence and rpfF transcript levels by citronellal treatment. However, an insignificant effect of 75 μM CIT treatment was observed on motB, flgE, pilA, estY, pglA, protease and lytic genes expression. Finally, the observations recorded were in confirmity with the virulence leaf clip test as lesion length was significantly decreased (39%) in CIT treatment as compared to the control leaves inoculated with only X. oryzae pv. oryzae. Overall, the findings obtained advocate the use of CIT for promising anti biofilm and anti virulence activity which in turn can be used for managing the blight disease in rice., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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34. Citral, a monoterpenoid aldehyde interacts synergistically with norfloxacin against methicillin resistant Staphylococcus aureus.
- Author
-
Gupta P, Patel DK, Gupta VK, Pal A, Tandon S, and Darokar MP
- Subjects
- Acyclic Monoterpenes, Aldehydes pharmacology, Animals, Drug Synergism, Mice, Microbial Sensitivity Tests, Staphylococcal Infections drug therapy, Anti-Bacterial Agents pharmacology, Methicillin-Resistant Staphylococcus aureus drug effects, Monoterpenes pharmacology, Norfloxacin pharmacology
- Abstract
Background: Staphylococcus aureus (SA), is a major human pathogen causing wide range of clinical infections, which has been further complicated by drug resistance like methicillin resistant S. aureus (MRSA), vancomycin intermediate S. aureus (VISA)/vancomycin resistant S. aureus (VRSA), etc. The present study was aimed at determining anti-staphylococcal potential of citral against drug resistant clinical isolates alone and in combination with antibiotics., Purpose: To assess the potential of citral in combination with norfloxacin in treating drug resistant infections of SA., Study Design: In the present study, synergistic interaction of citral and norfloxacin against drug resistant SA strains was evaluated. Further the efficacy and possible mechanism of action of the combination was also evaluated using in vitro and in vivo assays., Method: The anti-staphylococcal activity of each of the monoterpene and the antibiotic was determined in terms of MIC and the effective concentration of both compounds in combination was obtained by checkerboard assay. In vivo efficacy and oral acute toxicity was evaluated in Swiss albino mice model. To understand the mechanism of action, time-kill curve, bacteriolysis, leakage, membrane depolarization, salt tolerance and ethidium bromide efflux assays were performed., Results: Citral was found effective against clinical isolates of SA with MIC values ranging from 75 to 150 µg ml
-1 exhibiting bacteriostatic activity. Citral interacted synergistically, reducing MIC of norfloxacin up to 32-folds with FICI ≤ 0.50. Citral did not affect cell wall, but could damage cell membrane, inhibit efflux pump and affect the membrane potential. Citral could reduce the staphylococcal load of spleen and liver tissues in a dose-dependent manner which was further reduced when used in combination with norfloxacin. Citral did not exhibit any mortality or morbidity up to 500 mg kg-1 body weight and found to prolong the post-antibiotic effect of norfloxacin., Conclusion: Based on these observations, citral could be a lead candidate phytomolecule for further developing it into an anti-staphylococcal agent. The observations of combination study will help in reducing the burden of antibiotics leading to delayed resistance development., (Copyright © 2017 Elsevier GmbH. All rights reserved.)- Published
- 2017
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35. 1-Methyl-4-propan-2-ylbenzene from Thymus vulgaris Attenuates Cholinergic Dysfunction.
- Author
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Sammi SR, Trivedi S, Rath SK, Nagar A, Tandon S, Kalra A, and Pandey R
- Subjects
- Acetylcholinesterase genetics, Alzheimer Disease drug therapy, Animals, Caenorhabditis elegans, Cholinesterase Inhibitors pharmacology, Plant Extracts pharmacology, Receptors, Nicotinic drug effects, Benzene Derivatives pharmacology, Cholinergic Neurons drug effects, Propane analogs & derivatives, Propane pharmacology, Synaptic Transmission drug effects, Thymus Plant chemistry
- Abstract
Cholinergic dysfunction is manifested in a plethora of neurodegenerative and psychiatric disorders such as Alzheimer's, Parkinson's, and Huntington's diseases. The extent of cholinergic affliction is maximum in Alzheimer's disease which is a progressive neurodegenerative disorder involving death of cholinergic neurons. To this date, the therapeutic management of cholinergic dysfunction is limited to provide symptomatic relief through the use of acetylcholinesterase (Ache) inhibitors only. The present study elaborates the potential of thyme oil and its individual components in curtailing cholinergic deficits. We found that thyme oil augments neurotransmission by modulating synaptic acetylcholine (Ach) levels and nicotinic acetylcholine receptor activity, being orchestrated through upregulation of genes cho-1, unc-17 and unc-50. Studies on individual components revealed para-cymene (1-methyl-4-propan-2-ylbenzene) as the active component of thyme oil, contributing its effects through upregulation of cho-1, cha-1, unc-17 and unc-50, while downregulating ace-1 and ace-2. Interestingly, thymol and gamma-terpinene which although were devoid of any activity individually, exhibited significantly enhanced synaptic Ach levels and nicotinic acetylcholine receptor (nAchR) responsiveness, when administered in combination. Our findings advocate thyme oil and its constituents as potential candidates for amelioration of cholinergic dysfunction. The study is speculated to make a way for a new line of "phytomolecules-based drugs" from the diverse pool of natural compounds.
- Published
- 2017
- Full Text
- View/download PDF
36. A Review on Phytoconstituents and Biological activities of Cuscuta species.
- Author
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Ahmad A, Tandon S, Xuan TD, and Nooreen Z
- Subjects
- Anti-Infective Agents chemistry, Anti-Infective Agents pharmacology, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents pharmacology, Phytochemicals chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Cuscuta chemistry, Phytochemicals pharmacology
- Abstract
The genus Cuscuta belonging to the Cuscutaceae family comprises of about 100-170 species spread around the world. Although several species have been studied for their phytochemical characterization and biological activities but still many species are yet unexplored till date. Cuscuta are parasitic plants generally of yellow, orange, red or rarely green color. The Cuscuta species were reported rich in flavonoid and glycosidic constituents along with alkaloids, fatty acids, fixed oil, minerals, essential oil and others phytomolecules also etc. Flavonoids and other molecules of Cuscuta species were reported for different types of biological activities such as antiproliferative activity, antioxidant activity, anti-inflammatory, hepatoprotective, antimicrobial and anxiolytic activity, while some other flavonoids have exhibited potential antiviral and anticancer especially in ovarian and breast cancer activities. This review is an attempt to compile all the available data for the 24 different of Cuscuta species on the basis of different types of phytochemical constituents and biological studies as above., (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
- Published
- 2017
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37. Thyme Oil Reduces Biofilm Formation and Impairs Virulence of Xanthomonas oryzae .
- Author
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Singh A, Gupta R, Tandon S, and Pandey R
- Abstract
Xanthomonas oryzae pv. oryzae (Xoo), a common bacterial plant pathogen regulates its virulence and biofilm formation attribute via a chemical method of communication. Disabling this mechanism offers a promising alternative to reduce the virulence and pathogencity of the microorganism. In this study, the effect of thyme (THY) oil on Quorum Sensing mediated synthesis of various virulence factors and biofilm formation was analyzed. Treatment of Xoo with 500 ppm THY oil displayed a significant diminution in swimming, swarming, exopolysaccharide and xanthomonadin secretion. However, no effect was observed on bacterial growth kinetics and metabolic activity of the cells. Results were further authenticated by RT-qPCR as significant reduction in motA, motB , and flgE genes was observed upon THY oil treatment. Similarly, the expression of some extracellular enzyme genes such as endoglucanase, xylanase, cellobiosidase, and polygalacturonase was also found to be significantly reduced. However, biochemical plate assays revealed insignificant effect of 500 ppm THY oil on secretion of protease, cellulase, and lipase enzymes. The rpfF gene known to play a crucial role in the virulence of the phytopathogenic bacteria was also significantly reduced in the THY oil treated Xoo cells. HPTLC analysis further revealed significant reduction in DSF and BDSF signaling molecules when Xoo cells were treated with 500 ppm THY oil. Disease reduction was observed in in vitro agar plate assay as lesion length was reduced in THY oil treated Xoo cells when compared with the alone treatment. GC-MS result revealed thymol as the active and major component of THY oil which showed potential binding with rpfF gene. Application of 75 μM thymol resulted in downregulation of gumC, motA, estA , virulence acvB and pglA along with rpfF. The other genes such as cheD, flgA, cheY , and pilA , were not found to be significantly affected. Overall, the results clearly indicated THY oil and its active component Thymol to be a potential candidate for the development of anti-virulence agent which in future when applied in combination with conventional bactericides might not only help in lowering the dose of bactericides but also be successful in curbing the disease progression in rice.
- Published
- 2017
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38. Characterization and evaluation of bioactive polyphenolic constituents from Zanthoxylum armatum DC., a traditionally used plant.
- Author
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Nooreen Z, Singh S, Singh DK, Tandon S, Ahmad A, and Luqman S
- Subjects
- Anti-Bacterial Agents chemistry, Antioxidants chemistry, Bacteria drug effects, Flavonoids chemistry, Molecular Structure, Polyphenols chemistry, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Flavonoids pharmacology, Polyphenols pharmacology, Zanthoxylum chemistry
- Abstract
Zanthoxylum armatum or Timoor has been used in different traditional system of medicine due to its aromatic properties and also in the treatment of cancer, diarrhea and cholera. In the present investigation, four chemically distinct compounds namely Tambulin (6), Prudomestin (7), Ombuin (8) and 3, 4, 5, 3', 4', 5'-hexahydroxydiphenyl ether (9) have been isolated and quantified from the fruits. To explore the biological activities, we have further studied the antiproliferative, antimicrobial and antioxidant efficacy. Tambulin which was also found in maximum amount (0.125%) in fruits revealed significant antiproliferative activity (IC
50 37.96±0.36 to 48.7±0.21μg/mL) against breast, liver, colon and skin cancer cell lines corroborated by resilient binding interaction with SDH (-6.76Kcal/mol) and inhibition constant (Ki: 11.02μM). Hexane and ethyl acetate fraction exhibited moderate antibacterial efficacy (MIC: 250-1000μg/mL) against selected pathogenic microbes while Ombuin displayed broad spectrum antibacterial effect with MIC ranges from 125 to 500μg/mL. Total phenolic content (5.27±0.06 to 46.12±0.40mg/g of gallic acid equivalents), total flavonoids content (6.05±0.24 to 14.46±0.73mg/g of quercetin equivalents), ferric reducing power (42.35±0.85 to 62.52±0.66mg/g of ferrous sulfate equivalents) and percent free radical scavenging activity (59.56±0.38 to 64.85±1.78) were also estimated. Our findings infer that Tambulin exhibited significant antiproliferative activity whereas Ombuin was found to display broad spectrum antibacterial activity which adds one more positive attribute to its traditional usage., (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)- Published
- 2017
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39. Comparative transcriptome analysis reveals candidate genes for the biosynthesis of natural insecticide in Tanacetum cinerariifolium.
- Author
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Khan S, Upadhyay S, Khan F, Tandon S, Shukla RK, Ghosh S, Gupta V, Banerjee S, and Ur Rahman L
- Subjects
- Gene Ontology, Molecular Sequence Annotation, Proteomics, Sequence Analysis, Biological Products metabolism, Chrysanthemum cinerariifolium genetics, Chrysanthemum cinerariifolium metabolism, Gene Expression Profiling, Genes, Plant genetics, Insecticides metabolism, Pyrethrins metabolism
- Abstract
Background: Pyrethrins are monoterpenoids and consist of either a chrysanthemic acid or pyrethric acid with a rethrolone moiety. Natural pyrethrins are safe and eco-friendly while possessing strong insecticidal properties. Despite such advantages of commercial value coming with the eco-friendly tag, most enzymes/genes involved in the pyrethrin biosynthesis pathway remain unidentified and uncharacterized. Since the flowers of Tanacetum cinerariifolium are rich in major pyrethrins, next generation transcriptome sequencing was undertaken to compare the flowers and the leaves of the plant de novo to identify differentially expressed transcripts and ascertain which among them might be involved in and responsible for the differential accumulation of pyrethrins in T. cinerariifolium flowers., Results: In this first tissue specific transcriptome analysis of the non-model plant T. cinerariifolium, a total of 23,200,000 and 28,500,110 high quality Illumina next generation sequence reads, with a length of 101 bp, were generated for the flower and leaf tissue respectively. After functional enrichment analysis and GO based annotation using public protein databases such as UniRef, PFAM, SMART, KEGG and NR, 4443 and 8901 unigenes were identified in the flower and leaf tissue respectively. These could be assigned to 13344 KEGG pathways and the pyrethrin biosynthesis contextualized. The 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway was involved in the biosynthesis of acid moiety of pyrethrin and this pathway predominated in the flowers as compared to the leaves. However, enzymes related to oxylipin biosynthesis were found predominantly in the leaf tissue, which suggested that major steps of pyrethrin biosynthesis occurred in the flowers., Conclusions: Transcriptome comparison between the flower and leaf tissue of T. cinerariifolium provided an elaborate list of tissue specific transcripts that was useful in elucidating the differences in the expression of the biosynthetic pathways leading to differential presence of pyrethrin in the flowers. The information generated on genes, pathways and markers related to pyrethrin biosynthesis in this study will be helpful in enhancing the production of these useful compounds for value added breeding programs. Related proteome comparison to overlay our transcriptome comparison can generate more relevant information to better understand flower specific accumulation of secondary metabolites in general and pyrethrin accumulation in particular.
- Published
- 2017
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40. Synthesis of 3,5-dihydroxy-7,8-dimethoxy-2-(4-methoxyphenyl)benzopyran-4-one derivatives as anticancer agents.
- Author
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Singh S, Ahmad A, Raghuvanshi DS, Hasanain M, Agarwal K, Dubey V, Fatima K, Alam S, Sarkar J, Luqman S, Khan F, Tandon S, and Gupta A
- Subjects
- Apoptosis drug effects, Cell Line, Tumor, Humans, Inhibitory Concentration 50, Structure-Activity Relationship, Antineoplastic Agents chemical synthesis, Antineoplastic Agents pharmacology, Benzopyrans chemical synthesis, Benzopyrans pharmacology
- Abstract
Different alkyl amide (15a-l) and alkyl amine (16a-e) derivatives of 7,8-dimethoxy-3-hydroxy-2-(4-methoxyphenyl)benzopyran-4-one were synthesized and evaluated for their anticancer activity against five different cancer cell lines using SRB assay. Compounds 15e, 15i, 15j and 16a-e showed significant anticancer activity within the range of IC
50 2.58-34.86μM. The most promising molecule, 16c, was further analyzed for its effect on cell cycle and apoptosis of estrogen receptor positive cancer cells (MCF-7 cells) which showed that 16c triggered apoptosis in MCF-7 cells and arrested cells population at sub-G0 (apoptotic) and G2 M phase. In tubulin polymerization assay, 16c interfered with kinetics of tubulin polymerization., (Copyright © 2016 Elsevier Ltd. All rights reserved.)- Published
- 2016
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41. Pharmacological and phytochemical evaluation of Ocimum sanctum root extracts for its antiinflammatory, analgesic and antipyretic activities.
- Author
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Kumar A, Agarwal K, Maurya AK, Shanker K, Bushra U, Tandon S, and Bawankule DU
- Abstract
Background: Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) increases risk of having a range of gastrointestinal problems. Therefore, new anti-inflammatory, analgesic, antipyretic drugs having lesser side effects are being searched all overthe world as alternatives to NSAIDs., Aims: To evaluate the anti-inflammatory, analgesic and antipyretic profile of Ocimum sanctum root extracts., Materials and Methods: Anti-inflammatory profile of hexane (STH), chloroform (STC), ethyl acetate (STE), butanol (STB) and water (STW) extracts of OS was carried out by using carrageenan induced paw edema. STE a most active extract was further validated in dose dependent manner for anti-inflammatory, analgesic and antipyretic activity as well as oral toxicity profile in small laboratory animals. Identification of bioactives flux and chemical signature of most active fraction STE was developed by using the high-performance liquid chromatography fingerprinting., Results: An ethyl acetate fraction (STE) exhibit most potent anti-inflammatory activity followed by STB, STW, STC and STH. Dose response study of STE showed anti-inflammatory, analgesic and anti-pyretic potential in dose-dependent manner without any toxic effect at dose 2000 mg/kg. Chemical fingerprint revealed the presence of flavanoids., Conclusions: The present research revealed that STE possess anti-inflammatory, analgesic and anti-pyretic properties. However, future research is advocated to evaluate the pharmacological properties of isolated bioactive compounds.
- Published
- 2015
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42. In silico assay development for screening of tetracyclic triterpenoids as anticancer agents against human breast cancer cell line MCF7.
- Author
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Prakash O, Ahmad A, Tripathi VK, Tandon S, Pant AB, and Khan F
- Subjects
- Breast Neoplasms, Datasets as Topic, Female, Humans, MCF-7 Cells, Models, Molecular, Models, Theoretical, Reproducibility of Results, Structure-Activity Relationship, Workflow, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Computer Simulation, Drug Screening Assays, Antitumor methods, Triterpenes chemistry, Triterpenes pharmacology
- Abstract
Experimental activity of a compound on cancer cell line/target is mostly analyzed in the form of percentage inhibition at different concentration gradient and time of incubation. In this study a statistical model has been developed referred as in silico assay using support vector regression model, which can act with change in concentration gradient and time of incubation. This model is a function of concentration gradient, treatment hour and independent components; which calculate the percentage inhibition in combination of above three components. This model is designed to screen tetracyclic triterpenoids active against human breast cancer cell line MCF7. The model has been statistically validated, checked for applicability domain and predicted results were reconfirmed by MTT assay, for example Oenotheranstrol derivatives, OenA & B. Computational SAR, target and docking studies were performed to understand the cytotoxic mechanism of action of Oenotheranstrol compounds. The proposed in silico assay model will work for specific chemical family for which it will be optimized. This model can be used to analyze growth kinetics pattern on different human cancer cell lines for designed compounds.
- Published
- 2014
- Full Text
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43. Structure-activity relationships of monoterpenes and acetyl derivatives against Aedes aegypti (Diptera: Culicidae) larvae.
- Author
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Pandey SK, Tandon S, Ahmad A, Singh AK, and Tripathi AK
- Subjects
- Aedes growth & development, Animals, Larva growth & development, Molecular Structure, Structure-Activity Relationship, Aedes drug effects, Insecticides chemistry, Insecticides pharmacology, Larva drug effects, Monoterpenes chemistry, Monoterpenes pharmacology
- Abstract
Background: Dengue fever virus transmitted by Aedes aegypti causes lethal mortalities of human beings, and, because of the lack of any vaccine, management of this vector, especially with phytochemicals, is essential. In the present investigation, the structure-activity relationship of monoterpenes and their acetyl derivatives was studied to identify structural features that are responsible for mosquitocidal activity., Results: Derivatization of monoterpenes (eugenol, geraniol, linalool, L-menthol and terpeniole) followed by structure-activity relationship studies identified all five acetyl derivatives as having enhanced mosquitocidal activity against fourth-instar larvae of Aedes aegypti. Acetylation of the hydroxyl group in general increased activity in comparison with hydroxyl compounds. Based on LC50 values (ppm), the activities could be placed in the following order: eugenyl acetate (50.2) > linalyl acetate (119.7) > terpinyl acetate (287.1) > menthyl acetate (308.4) > geranyl acetate (325.5), as compared with monoterpenoids: eugenol (82.8) > linalool (242.6) > terpineol (331.7) > L-menthol (365.8) > geraniol (415.0). In eugenyl acetate, the presence of an aromatic ring and a side chain with an allylic double bond makes it most effective., Conclusion: Bioactive functional groups identified in the study may contribute to the understanding of larvicidal activity of acetyl derivatives and may help in the development of ecofriendly mosquito larvicidal compounds., (© 2013 Society of Chemical Industry.)
- Published
- 2013
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44. Antiaging, antistress and ROS scavenging activity of crude extract of Ocimum sanctum (L.) in Caenorhabditis elegans (Maupas, 1900).
- Author
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Pandey R, Gupta S, Shukla V, Tandon S, and Shukla V
- Subjects
- Animals, Caenorhabditis elegans growth & development, Caenorhabditis elegans metabolism, Caenorhabditis elegans Proteins genetics, Caenorhabditis elegans Proteins metabolism, Cell Proliferation, Chemotaxis drug effects, Environment, HSP70 Heat-Shock Proteins genetics, HSP70 Heat-Shock Proteins metabolism, Hot Temperature, Hydrogen Peroxide metabolism, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction drug effects, Sirtuins genetics, Sirtuins metabolism, Aging drug effects, Antioxidants pharmacology, Caenorhabditis elegans drug effects, Complex Mixtures pharmacology, Free Radical Scavengers pharmacology, Ocimum chemistry, Oxidative Stress drug effects
- Abstract
Since aging is the most important risk factor for variety of diseases, the discovery of a wide range of chemical modulators of aging in model organisms encourages new strategies for targeting age associated diseases. Simple genetic manipulation leads to long-lived and healthy animals, so any compound which could have similar effect would prove a boon to mankind. In the present study, effect of different pharmacological doses (1.0, 0.1, 0.01 and 0.001 mg/mL) of O. sanctum crude extract were used to determine their impact on life span, thermotolerance and ROS scavenging activities in C. elegans. The results revealed that 1 mg/mL of O. sanctum extract significantly extended the life span of C. elegans. The extract also proved to be a strong free radical scavenger and increased resistance against thermal stress. It is also suggested that the protective and life span extending action of the crude extract is not only due to its antioxidant capacity but may also be mediated by modulation of some signaling pathways. Thus, in addition to all the known medicinal property of Ocimum, it is capable of increasing stress tolerance and life span in C. elegans.
- Published
- 2013
45. Baccoside A suppresses epileptic-like seizure/convulsion in Caenorhabditis elegans.
- Author
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Pandey R, Gupta S, Tandon S, Wolkenhauer O, Vera J, and Gupta SK
- Subjects
- Animals, Caenorhabditis elegans physiology, Disease Models, Animal, Epilepsy drug therapy, Hot Temperature, Saponins pharmacology, Seizures drug therapy, Triterpenes pharmacology, Bacopa chemistry, Caenorhabditis elegans drug effects, Neurons drug effects, Phytotherapy methods, Plant Extracts pharmacology
- Abstract
The 1 mm long Caenorhabditis elegans is one of the prime research tools to study different human neurodegenerative diseases. We have considered the case in which increase in the surrounding temperature of this multicellular model leads to abnormal bursts of neuronal cells that can be linked to seizure or convulsion. The induction of such seizure/convulsion mechanism was done by gradually increasing the temperature with 1x buffer (100 mM NaCl, 50 mM MgCl(2)) in adult C. elegans. In the present experiment it is demonstrated that Baccoside A can significantly reduce the seizure/convulsion in C. elegans at higher temperatures (26-28+/-1 degrees C). Furthermore, in T-type Ca(2+) channel cca-1 mutant worms, no convulsion was recorded. Our experimental results suggest that plant molecules from Bacopa monnieri may be useful in suppressing the seizure/convulsion in worms., (2010 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2010
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46. Identification and quantification of two antihepatotoxic coumarinolignoids cleomiscosin A and cleomiscosin B in the seeds of Cleome viscosa using liquid chromatography-tandem mass spectrometry.
- Author
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Chattopadhyay SK, Kumar S, Kaur R, Tandon S, and Rane S
- Subjects
- Coumarins chemistry, Seeds chemistry, Chromatography, High Pressure Liquid methods, Cleome chemistry, Coumarins analysis, Spectrometry, Mass, Electrospray Ionization methods, Tandem Mass Spectrometry methods
- Abstract
A sensitive liquid chromatography/electrospray ionization tandem mass spectrometric (LC/ESI-MS/MS) method was developed for the identification and quantification of two antihepatotoxic coumarinolignoids cleomiscosin A and cleomiscosin B in different extracts of the seeds of Cleome viscosa. The separation of cleomiscosin A and cleomiscosin B was achieved on an RP(18) column using a solvent system consisting of a mixture of acetonitrile-methanol (1:2, v/v) and acetonitrile-water-formic acid (5:95:0.3, v/v) as a mobile phase in a gradient elution mode. A multiple-reaction monitoring (MRM) method was developed for quantification of cleomiscosin A and cleomiscosin B in the seed extracts of Cleome viscosa. On the basis of signal-to-noise ratio of 3, the limit of detection in MRM mode for cleomiscosin A and cleomiscosin B were 1.0 and 4.0 ng/mL respectively. The method was validated in terms of linearity, accuracy and precision for 6 days. The method developed was found to be useful for identification and quantification of cleomiscosin A and cleomiscosin B in the different extracts of the seeds of Cleome viscosa.
- Published
- 2009
- Full Text
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47. Demethoxycurcumin and its semisynthetic analogues as antitubercular agents.
- Author
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Agrawal DK, Saikia D, Tiwari R, Ojha S, Shanker K, Kumar JK, Gupta AK, Tandon S, Negi AS, and Khanuja SP
- Subjects
- Antitubercular Agents isolation & purification, Curcumin isolation & purification, Curcumin pharmacology, Diarylheptanoids, Molecular Structure, Plant Extracts isolation & purification, Rhizome, Antitubercular Agents pharmacology, Curcuma chemistry, Curcumin analogs & derivatives, Mycobacterium tuberculosis drug effects, Plant Extracts pharmacology
- Abstract
Demethoxycurcumin, isolated from the rhizomes of Curcuma longa, was found to possess antitubercular activity against Mycobacterium tuberculosis H (37)Rv strain at 200 microg/mL. Derivatisation of this active principle yielded a potent agent 6, exhibiting considerable activity with a minimum inhibitory concentration (MIC) value of 7.8 microg/mL. H (37)Rv:Mycobacterium tuberculosis H (37)Rv strain MIC:minimum inhibitory concentration.
- Published
- 2008
- Full Text
- View/download PDF
48. High-performance liquid chromatography and LC-ESI-MS method for the identification and quantification of two biologically active isomeric coumarinolignoids cleomiscosin A and cleomiscosin B in different extracts of Cleome viscosa.
- Author
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Chattopadhyay SK, Kumar S, Tripathi S, Kaur R, Tandon S, and Rane S
- Subjects
- Coumarins chemistry, Isomerism, Molecular Structure, Reproducibility of Results, Chromatography, High Pressure Liquid methods, Cleome chemistry, Coumarins analysis, Plant Extracts chemistry, Spectrometry, Mass, Electrospray Ionization methods
- Abstract
A rapid, sensitive and simple reverse-phase high-performance liquid chromatographic-electrospray ionization-mass spectrometry method for simultaneous determination of cleomiscosin A and cleomiscosin B has been developed and validated. The isomeric coumarinolignoids cleomiscosin A (1) and cleomiscosin B (2) were separated on a Waters symmetry C(18) column with a solvent system composed of acetonitrile-methanol (1:2) and acetic acid-water (0.5:99.5) in a gradient elution mode. The absorption at 326 nm was chosen as the measuring wavelength in which resolution and baseline separation of compounds 1 and 2 could be obtained. The identity of the two isomeric compounds 1 and 2 in the samples were determined on a triple quadrupole mass spectrometer with ESI interface operating in the positive mode. Calibration curves were linear (r(2) > 0.993) over the concentration range 20-200 microg/mL for cleomiscosin A and 10-200 microg/mL for cleomiscosin B with acceptable accuracy and precision, respectively. The intra-day and inter-day precision were 1.13 and 0.82% for cleomiscosin A and 1.78 and 1.28% for cleomiscosin B, respectively. The validated method was successfully applied for the analysis of the above two compounds in different extracts of Cleome viscosa.
- Published
- 2008
- Full Text
- View/download PDF
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