1. Enhanced brain-targeting and efficacy of cannabidiol via RVG-Exo/CBD nanodelivery system.
- Author
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Li Y, Chen Z, Guo J, Meng D, Pang X, Sun Z, Pu L, Yang S, Yang M, and Peng Y
- Subjects
- Animals, Mice, Male, Glycoproteins chemistry, Glycoproteins metabolism, Glycoproteins administration & dosage, Drug Delivery Systems methods, Peptide Fragments, Viral Proteins, Cannabidiol administration & dosage, Cannabidiol chemistry, Cannabidiol pharmacology, Brain metabolism, Brain drug effects
- Abstract
This study introduces an innovative brain-targeted drug delivery system, RVG-Exo/CBD, utilizing rabies virus glycoprotein (RVG)-engineered exosomes for encapsulating cannabidiol (CBD). The novel delivery system was meticulously characterized, confirming the maintenance of exosomal integrity, size, and successful drug encapsulation with a high drug loading rate of 83.0 %. Evaluation of the RVG-Exo/CBD's brain-targeting capability demonstrated superior distribution and retention in brain tissue compared to unmodified exosomes, primarily validated through in vivo fluorescence imaging. The efficacy of this delivery system was assessed using a behavioral sensitization model in mice, where RVG-Exo/CBD notably suppressed methamphetamine-induced hyperactivity more effectively than CBD alone, indicating a reduction in effective dose and enhanced bioavailability. Overall, the RVG-Exo/CBD system emerges as a promising strategy for enhancing the therapeutic efficacy and safety of CBD, particularly for neurological applications, highlighting its potential for addressing the limitations associated with traditional CBD administration in clinical settings., Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
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