43 results on '"Fu JG"'
Search Results
2. Free PSA performs better than total PSA in predicting prostate volume in Chinese men with PSA levels of 2.5-9.9 ng ml -1 .
- Author
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Huang MP, Tang P, Klein CS, Wei XH, Du W, Fu JG, Huang TH, Chen H, and Xie KJ
- Subjects
- Male, Humans, Prostate, East Asian People, ROC Curve, Prostate-Specific Antigen, Prostatic Neoplasms diagnosis
- Abstract
This study investigated whether free prostate-specific antigen (fPSA) performs better than total PSA (tPSA) in predicting prostate volume (PV) in Chinese men with different PSA levels. A total of 5463 men with PSA levels of <10 ng ml
-1 and without prostate cancer diagnosis were included in this study. Patients were classified into four groups: PSA <2.5 ng ml-1 , 2.5-3.9 ng ml-1 , 4.0-9.9 ng ml-1 , and 2.5-9.9 ng ml-1 . Pearson/Spearman's correlation coefficient (r) and receiver operating characteristic (ROC) curves were used to evaluate the ability of tPSA and fPSA to predict PV. The correlation coefficient between tPSA and PV in the PSA <2.5 ng ml-1 cohort (r = 0.422; P < 0.001) was markedly higher than those of the cohorts with PSA levels of 2.5-3.9 ng ml-1 , 4.0-9.9 ng ml-1 , and 2.5-9.9 ng ml-1 (r = 0.114, 0.167, and 0.264, respectively; all P ≤ 0.001), while fPSA levels did not differ significantly among different PSA groups. Area under ROC curve (AUC) analyses revealed that the performance of fPSA in predicting PV ≥40 ml (AUC: 0.694, 0.714, and 0.727) was better than that of tPSA (AUC = 0.545, 0.561, and 0.611) in men with PSA levels of 2.5-3.9 ng ml-1 , 4.0-9.9 ng ml-1 , and 2.5-9.9 ng ml-1 , respectively, but not at PSA levels of <2.5 ng ml-1 (AUC: 0.713 vs 0.720). These findings suggest that the relationship between tPSA and PV may vary with PSA level and that fPSA is more powerful at predicting PV only in the ''gray zone'' (PSA levels of 2.5-9.9 ng ml-1 ), but its performance was similar to that of tPSA at PSA levels of <2.5 ng ml-1 ., Competing Interests: None- Published
- 2023
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3. Palladium-catalyzed cross-coupling of unreactive C(sp 3 )-H bonds with azole C(sp 2 )-H bonds by using bromide as a traceless directing group.
- Author
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Chen YZ, Fu JG, Ji XM, Zhang SS, and Feng CG
- Subjects
- Azoles, Catalysis, Ligands, Bromides, Palladium chemistry
- Abstract
A palladium-catalyzed intermolecular cross-coupling of unreactive C(sp
3 )-H bonds and azole C(sp2 )-H bonds with bromide as a traceless directing group is described. The judicious selection of the bulky and electron-rich phosphine ligand is the key for the success of this cascade process. The protocol features a broad substrate scope, excellent regioselectivity, and good functional group tolerance.- Published
- 2022
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4. Phosphorylation of C(sp 3 )-H Bonds via 1,4-Palladium Migration.
- Author
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Ji XM, Chen YZ, Fu JG, Zhang SS, and Feng CG
- Subjects
- Catalysis, Ligands, Phosphorylation, Carbon chemistry, Palladium chemistry
- Abstract
1,4-Palladium migration has emerged as a reliable method for directed C-H functionalization. In contrast to coupling with carbon nucleophiles, limited examples with heteroatom nucleophiles have been reported. Herein we report a palladium-catalyzed intermolecular C(sp
3 )-H phosphorylation reaction via 1,4-palladium migration, which is often difficult because of the strong coordination of phosphorus reagents to palladium catalysts. Phosphorylation of C(sp3 )-H bonds is accomplished in good reaction yields with excellent regioselectivity. The judicious selection of the phosphine ligand proved to be the key to the success of this cascade process.- Published
- 2022
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5. Bacteria - Anchoring Hybrid Liposome Capable of Absorbing Multiple Toxins for Antivirulence Therapy of Escherichia coli Infection.
- Author
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Jiang L, Zhu Y, Luan P, Xu J, Ru G, Fu JG, Sang N, Xiong Y, He Y, Lin GQ, Wang J, Zhang J, and Li R
- Subjects
- Animals, Endotoxins, Erythrocyte Membrane, Liposomes, Mice, Escherichia coli Infections drug therapy, Escherichia coli O157
- Abstract
Antivirulence therapy by cell membrane coated nanoparticles has shown promise against bacterial infections. However, current approaches remain unsatisfactory when facing Escherichia coli ( E. coli ) infections, since the E. coli secretes multiple bacterial toxins including endotoxins and exotoxins that are challenging to eliminate simultaneously. What is worse, the absorptive scavengers normally rely on random contact of the diffuse toxins, which is not efficient. For the current cell membrane coated platform, the single type of cell membrane cannot fully meet the detoxing requirement facing multiple toxins. To address these problems, a polymyxin B (PMB)-modified, red blood cell (RBC)-mimetic hybrid liposome (P-RL) was developed. The P-RL was fabricated succinctly through fusion of PMB-modified lipids and the RBC membranes. By the strong interaction between PMB and the E. coli membrane, P-RL could attach and anchor to the E. coli ; attributed to the fused RBC membrane and modified PMB, the P-RL could then efficiently neutralize both endotoxins and exotoxins from the toxin fountainhead. In vitro and in vivo results demonstrated the P-RL had a significant anchoring effect to E. coli . Moreover, compared with the existing RBC vesicles or PMB-modified liposomes, P-RL exhibited a superior therapeutic effect against RBC hemolysis, macrophage activation, and a mixed-toxin infection in mice. Potently, P-RL could inhibit E. coli O157:H7-induced skin damage, intestinal infection, and mouse death. Overall, the P-RL could potentially improve the detoxing efficiency and markedly expand the detoxification spectrum of current antivirulence systems, which provides different insights into drug-resistant E. coli treatment.
- Published
- 2021
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6. Successful treatment of encrusted cystitis: A case report and review of literature.
- Author
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Fu JG and Xie KJ
- Abstract
Background: Encrusted cystitis (EC) is a chronic inflammation of the bladder associated with mucosal encrustations. Early diagnosis and optimal treatment are not well established. Here, we report a case of EC successfully treated with com-bination therapy., Case Summary: A 27-year-old man presented with frequency, urgency, dysuria, gross hematuria and suprapubic pain for 2 mo. He was diagnosed with EC based on characteristic calcifications of the bladder wall (most of them were struvite), cystoscopy and histopathological examination. He was cured after combined therapy of elimination of encrustations, bladder instillation of hyaluronic acid and injection of botulinum-A neurotoxin into bladder submucosal tissue., Conclusion: Bladder instillation of hyaluronic acid and injection of botulinum-A neurotoxin into the bladder submucosal tissue can be used for treatment of EC., Competing Interests: Conflict-of-interest statement: The authors have nothing to disclose., (©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.)
- Published
- 2020
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7. Silver-promoted synthesis of vinyl sulfones from vinyl bromides and sulfonyl hydrazides in water.
- Author
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Zhang G, Fu JG, Zhao Q, Zhang GS, Li MY, Feng CG, and Lin GQ
- Abstract
The synthesis of vinyl sulfones via silver-promoted cross-coupling of vinyl bromides with sulfonyl hydrazides was realized. Water was used as the sole solvent. Multisubstituted vinyl sulfones were easily prepared with excellent alkyl group tolerance. A mechanism involving nucleophilic attack of a sulfinate anion was proposed.
- Published
- 2020
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8. Comparison of geometrical uncertainties in the radiotherapy for various treatment sites with two different immobilization marking methods.
- Author
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Pan JH, Yu DD, Song QB, Ruan CL, Fu JG, Wu B, Ma GD, Wang LZ, Lei Y, and Han G
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Body Mass Index, Cone-Beam Computed Tomography, Female, Humans, Male, Middle Aged, Neoplasms diagnostic imaging, Neoplasms radiotherapy, Patient Positioning, Prospective Studies, Radiotherapy Setup Errors, Young Adult, Immobilization, Radiotherapy Planning, Computer-Assisted methods, Radiotherapy, Image-Guided methods
- Abstract
Objective: The skin marking method (SMM) and bow-form-ruler marking method (BFRM) are two commonly used patient marking methods in mainland China. This study aims to evaluate SMM and BFRM by comparing the inter-fraction setup errors from using these two methods together with vacuum cushion immobilization in patients underwent radiotherapy for different treatment sites., Materials and Methods: Eighteen patients diagnosed with pelvic, abdominal and thoracic malignant tumors (with 6 patients per treatment site) were enrolled in this prospective study. All patients were immobilized with vacuum cushion. Each patient was marked by both SMM and BFRM before computed tomography (CT) simulation. Target location was verified by cone beam CT images with displacements assessed prior to each sampled treatment session. The localization errors in three translational and three rotational directions were recorded and analyzed., Results: Images from 108 fractions in 18 patients produced 324 translational and 324 rotational comparisons for SMM and BFRM. The setup errors of all treatment sites showed no difference in two marking methods in any directions (p > 0.05). In subgroups of treatment site analysis, SMM significantly lessened the lateral and yaw setup errors compared to BFRM in the pelvic sites (0.39±1.85 mm vs -1.28±1.13 mm, p < 0.01 and -0.19±0.59° vs -0.61±0.59°, p < 0.05). However, in the abdominal subgroup, BFRM was superior to SMM for reduced vertical errors (0.17±2.73 mm vs 2.28±3.16 mm, p < 0.05). For the underweight or obese patients (with Body Mass Index, BMI < 18.5 or BMI≥24), SMM resulted in less yaw errors compared to BFRM (-0.05±0.38° vs -0.43±0.48°, p < 0.05). No significant difference between SMM and BFRM in setup errors of normal weighted patients (18.5≤BMI < 24) was observed for all three studied treatment sites., Conclusions: This study shows no significant difference in patient setup errors for various treatment sites between SMM and BFRM in general. SMM may be suitable for the pelvic tumor and patients with BMI < 18.5 or BMI≥24, while BFRM is recommended for the abdominal tumor sites.
- Published
- 2019
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9. Heterogeneous responses and resistant mechanisms to crizotinib in ALK-positive advanced non-small cell lung cancer.
- Author
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Kang J, Chen HJ, Zhang XC, Su J, Zhou Q, Tu HY, Wang Z, Wang BC, Zhong WZ, Yang XN, Chen ZH, Ding Y, Wu X, Wang M, Fu JG, Yang Z, Zhang X, Shao YW, Wu YL, and Yang JJ
- Subjects
- Anaplastic Lymphoma Kinase antagonists & inhibitors, Biomarkers, Tumor, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung mortality, Crizotinib therapeutic use, DNA Mutational Analysis, Gene Expression Profiling, Humans, Lung Neoplasms drug therapy, Lung Neoplasms mortality, Mutation, Neoplasm Metastasis, Neoplasm Staging, Prognosis, Protein Kinase Inhibitors therapeutic use, Anaplastic Lymphoma Kinase genetics, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung pathology, Crizotinib pharmacology, Drug Resistance, Bacterial genetics, Lung Neoplasms genetics, Lung Neoplasms pathology, Protein Kinase Inhibitors pharmacology
- Abstract
Background: ALK-tyrosine kinase inhibitors (TKIs) have been proven effective for treating ALK-positive non-small cell lung cancer (NSCLC), although patients present with variable responses and disease progression courses. The detailed underlying molecular mechanisms require further investigation to yield a better prognosis., Methods: Targeted next-generation sequencing (NGS) mutation profiling was performed on samples from 42 NSCLC patients confirmed positive for ALK rearrangements by fluorescence in situ hybridization or immunohistochemistry who experienced disease progression after crizotinib treatment., Results: ALK rearrangements were not confirmed in six patients (14%) with other potential oncogenic drivers identified by NGS, who therefore did not respond to crizotinib and had significantly shorter overall survival (OS) compared to NGS ALK -positive patients. Fifteen ALK activating mutations were detected in 8 out of 26 post-treatment samples (31%), among which ALK L1196M and G1269A were the most common acquired mutations detected in half of the patients with ALK activating mutations. Dynamic monitoring of the genetic evolution in one patient revealed both spatial and temporal heterogeneity of resistant mechanisms during different ALK-TKI treatment courses. Activation of ALK downstream or bypass pathways was detected in patients without ALK activating mutations, such as genetic alterations in PIK3CA, MET, and KRAS. Interestingly, we identified two patients with acquired mutations in the DNA mismatch repair gene POLE, which resulted in a dramatically increased tumor mutation burden, and might contribute to the poor response to crizotinib., Conclusions: Heterogeneous resistant mechanisms have been identified and correlate to diverse responses to crizotinib. Comprehensive and dynamic mutation profiling is required to better predict clinical outcomes., (© 2018 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.)
- Published
- 2018
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10. [Genetic characterization of norovirus isolated in an outbreak of gastroenteritis in Jiangsu province].
- Author
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Fu JG, Shi C, Sha D, Shi P, Bao CJ, and Ai J
- Subjects
- Caliciviridae Infections diagnosis, Caliciviridae Infections epidemiology, Capsid Proteins, Gastroenteritis epidemiology, Genotype, Humans, Norovirus isolation & purification, Phylogeny, RNA, Viral genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Disease Outbreaks, Gastroenteritis diagnosis, Norovirus genetics
- Abstract
Objective: To analyze the genetic characterization of norovirus isolated in an outbreak of gastroenteritis in Jiangsu province. Methods: Extracted viral RNA from the swab samples of cases of acute gastroenteritis outbreak in Jiangsu province on December 16-27, 2016 was reversely transcribed to cDNA, and partial RNA-dependent RNA polymerase sequence and complete capsid sequence (VP1) were amplified by RT-PCR. Amplification products were sequenced for the analysis of genetic characteristics. Results: Based on sequence alignment, the variant shared a high level of identity with the strain GⅡ.g isolated in Spain and Finland (98.7%) in the RNA-dependent RNA polymerase region, and with the strain GⅡ.1 isolated in American (99.4%) in the VP1. The recombination was determined by using software Simplot, and the breakpoint of recombination was located in the ORF1/2 overlap region at position 5 106 of VP1. The result of amino acids alignment in capsid region showed that there were no mutations in the amino acids of the predicted epitopes and receptor binding site Ⅰ-Ⅲ, but a unique amino acid change was detected at position 132 (N-S). Conclusion: The norovirus isolated in the outbreak of gastroenteritis in Jiangsu province was a rare recombinant norovirus variant GⅡ.g-GⅡ.1.
- Published
- 2018
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11. Outbreaks of acute gastroenteritis associated with a re-emerging GII.P16-GII.2 norovirus in the spring of 2017 in Jiangsu, China.
- Author
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Fu JG, Shi C, Xu C, Lin Q, Zhang J, Yi QH, Zhang J, Bao CJ, Huo X, Zhu YF, Ai J, and Xing Z
- Subjects
- Acute Disease, China epidemiology, Genes, Viral, Humans, Norovirus classification, Norovirus genetics, Norovirus pathogenicity, Phylogeny, Viral Proteins genetics, Caliciviridae Infections epidemiology, Disease Outbreaks, Gastroenteritis epidemiology, Norovirus isolation & purification
- Abstract
A total of 64 acute gastroenteritis outbreaks with 2,953 patients starting in December of 2016 and occurring mostly in the late spring of 2017 were reported in Jiangsu, China. A recombinant GII.P16-GII.2 norovirus variant was associated with 47 outbreaks (73.4%) for the gastroenteritis epidemic, predominantly occurring in February and March of 2017. Sequence analysis of the RNA-dependent RNA polymerase (RdRp) and capsid protein of the viral isolates from these outbreaks confirmed that this GII.P16-GII.2 strain was the GII.P16-GII.2 variant with the intergenotypic recombination, identified in Taiwan, Hong Kong, and other cities in China in 2016. This GII.P16-GII.2 recombinant variant appeared to a re-emerging strain, firstly identified in 2011-2012 from Japan and USA but might be independently originated from other GII.P16-GII.2 variants for sporadic and outbreaks of gastroenteritis in Japan and China before 2016. Further identification of unique amino acid mutations in both VP1 and RdRp of NoV strain as shown in this report may provide insight in explaining its structural and antigenic changes, potentially critical for the variant recombinant to gain its predominance in causing regional and worldwide epidemics.
- Published
- 2017
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12. Germination Response of Four Alien Congeneric Amaranthus Species to Environmental Factors.
- Author
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Hao JH, Lv SS, Bhattacharya S, and Fu JG
- Subjects
- Amaranthus classification, Amaranthus growth & development, China, Hydrogen-Ion Concentration, Light, Osmosis, Sodium Chloride administration & dosage, Species Specificity, Amaranthus physiology, Germination
- Abstract
Seed germination is the key step for successful establishment, growth and further expansion of population especially for alien plants with annual life cycle. Traits like better adaptability and germination response were thought to be associated with plant invasion. However, there are not enough empirical studies correlating adaptation to environmental factors with germination response of alien invasive plants. In this study, we conducted congeneric comparisons of germination response to different environmental factors such as light, pH, NaCl, osmotic and soil burials among four alien amaranths that differ in invasiveness and have sympatric distribution in Jiangsu Province, China. The data were used to create three-parameter sigmoid and exponential decay models, which were fitted to cumulative germination and emergence curves. The results showed higher maximum Germination (Gmax), shorter time for 50% germination (G50) and the rapid slope (Grate) for Amaranthus blitum (low-invasive) and A. retroflexus (high-invasive) compare to intermediately invasive A. spinosus and A. viridis in all experimental regimes. It indicated that germination potential does not necessarily constitute a trait that can efficiently distinguish highly invasive and low invasive congeners in four Amaranthus species. However, it was showed that the germination performances of four amaranth species were more or less correlated with their worldwide distribution area. Therefore, the germination performance can be used as a reference indicator, but not an absolute trait for invasiveness. Our results also confirmed that superior germination performance in wide environmental conditions supplementing high seed productivity in highly invasive A. retroflexus might be one of the reasons for its prolific growth and wide distribution. These findings lay the foundation to develop more efficient weed management practice like deep burial of seeds by turning over soil and use of tillage agriculture to control these invasive weed species., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2017
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13. Characterization of the new GII.17 norovirus variant that emerged recently as the predominant strain in China.
- Author
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Jin M, Zhou YK, Xie HP, Fu JG, He YQ, Zhang S, Jing HB, Kong XY, Sun XM, Li HY, Zhang Q, Li K, Zhang YJ, Zhou DQ, Xing WJ, Liao QH, Liu N, Yu HJ, Jiang X, Tan M, and Duan ZJ
- Subjects
- Blood Group Antigens genetics, Blood Group Antigens metabolism, Caliciviridae Infections epidemiology, Caliciviridae Infections genetics, Caliciviridae Infections metabolism, China epidemiology, Disease Outbreaks, Evolution, Molecular, Feces virology, Gastroenteritis epidemiology, Gastroenteritis virology, Humans, Norovirus classification, Norovirus genetics, Phylogeny, Caliciviridae Infections virology, Norovirus isolation & purification
- Abstract
Human noroviruses are the most important viral pathogens causing epidemic acute gastroenteritis, in which the GII.4 viruses have been predominant worldwide for the past decades. During 2014-2015 winter season, a new GII.17 variant emerged as the predominant virus in China surpassing the GII.4 virus in causing significantly increased acute gastroenteritis outbreaks. Genome sequences of the new GII.17 variant was determined and compared with other GII.17 noroviruses, revealing residue substitutions at specific locations, including the histo-blood group antigen-binding site and the putative antigenic epitopes. Further study of GII.17 outbreaks focusing on host susceptibility showed that the new GII.17 variant infected secretor individuals of A, B, O and Lewis types. Accordingly, the P particles of the new GII.17 variant bound secretor saliva samples of A, B, O and Lewis types with significantly higher binding signals than those of the P particles of the previous GII.17 variants. In addition, human sera collected from the outbreaks exhibited stronger blockade against the binding of the new GII.17 P particles to saliva samples than those against the binding between the P particles of previous GII.17 variants and saliva samples. Taken together, our data strongly suggested that the new GII.17 variant gained new histo-blood group antigen-binding ability and antigenic features, which may contribute to its predominance in causing human norovirus epidemics.
- Published
- 2016
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14. An asymmetric approach to bicyclo[2.2.1]heptane-1-carboxylates via a formal [4 + 2] cycloaddition reaction enabled by organocatalysis.
- Author
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Fu JG, Shan YF, Sun WB, Lin GQ, and Sun BF
- Abstract
An organocatalytic formal [4 + 2] cycloaddition reaction has been realized that permits rapid access to a wide range of bicyclo[2.2.1]heptane-1-carboxylates in a highly enantioselective manner from simple starting materials under mild and operationally simple conditions.
- Published
- 2016
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15. Molecular epidemiology of genogroup II norovirus infection among hospitalized children with acute gastroenteritis in Suzhou (Jiangsu, China) from 2010 to 2013.
- Author
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Fu JG, Ai J, Zhang J, Wu QB, Qi X, Ji H, Jin M, Liu C, Wang SJ, Shan J, Bao CJ, Tang FY, and Zhu YF
- Subjects
- Acute Disease, Capsid Proteins genetics, Child, Preschool, China epidemiology, Epidemics, Epidemiological Monitoring, Feces virology, Female, Fever virology, Genetic Variation, Genotype, Hospitalization, Humans, Infant, Infant, Newborn, Male, Norovirus classification, Norovirus pathogenicity, Phylogeny, Prevalence, RNA, Viral genetics, Real-Time Polymerase Chain Reaction, Seasons, Sequence Analysis, DNA, Caliciviridae Infections epidemiology, Caliciviridae Infections virology, Gastroenteritis virology, Norovirus genetics
- Abstract
Noroviruses (NoVs) are the most common cause of acute gastroenteritis in both sporadic and outbreak cases. Genotyping and recombination analyses were performed in order to help getting more knowledge of the distribution and genetic diversity of NoVs in Suzhou, located in Jiangsu province of China. All stool samples were collected from hospitalized children younger than 5 years old with acute gastroenteritis. For genotyping, the open reading frame (ORF) 1 and ORF2 were partially amplified and sequenced. 26.9% of stool samples were positive for genogroup II NoVs. The most common genotype was GII.4 and its variants included Den Haag-2006b, New Orleans-2009, and Sydney-2012. The Den Haag-2006b variants predominated during 2010-2012. In 2013, it was replaced by the Sydney-2012 variant. The second most common genotype was GII.12/GII.3. NoVs could be detected throughout the year, with GII.4 and GII.12/GII.3 coexisting during the cold months, and GII.4 was the main genotype during the warm months. The highest prevalence of NoV was detected in young children aged <24 months. Patients infected with GII.4 had a higher chance of getting moderate fever than other NoV-positive patients, while those infected with GII.12/GII.3 tended to have a mild degree of fever. NoV is an important pathogen responsible for viral gastroenteritis among children in Suzhou. Analyses of NoV circulating between 2010 and 2013 revealed a change of predominant variant of NoV GII.4 in each epidemic season and intergenotype recombinant strains represented an important part., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
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16. Interfacial interactions between plastic particles in plastics flotation.
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Wang CQ, Wang H, Gu GH, Fu JG, Lin QQ, and Liu YN
- Subjects
- Hydrophobic and Hydrophilic Interactions, Models, Theoretical, Particulate Matter chemistry, Plastics chemistry, Refuse Disposal, Water Pollutants, Chemical chemistry
- Abstract
Plastics flotation used for recycling of plastic wastes receives increasing attention for its industrial application. In order to study the mechanism of plastics flotation, the interfacial interactions between plastic particles in flotation system were investigated through calculation of Lifshitz-van der Waals (LW) function, Lewis acid-base (AB) Gibbs function, and the extended Derjaguin-Landau-Verwey-Overbeek potential energy profiles. The results showed that van der Waals force between plastic particles is attraction force in flotation system. The large hydrophobic attraction, caused by the AB Gibbs function, is the dominant interparticle force. Wetting agents present significant effects on the interfacial interactions between plastic particles. It is found that adsorption of wetting agents promotes dispersion of plastic particles and decreases the floatability. Pneumatic flotation may improve the recovery and purity of separated plastics through selective adsorption of wetting agents on plastic surface. The relationships between hydrophobic attraction and surface properties were also examined. It is revealed that there exists a three-order polynomial relationship between the AB Gibbs function and Lewis base component. Our finding provides some insights into mechanism of plastics flotation., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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17. Characterization of interleukin-1β as a proinflammatory cytokine in grass carp (Ctenopharyngodon idella).
- Author
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Bo YX, Song XH, Wu K, Hu B, Sun BY, Liu ZJ, and Fu JG
- Subjects
- Aeromonas hydrophila physiology, Amino Acid Sequence, Animals, Base Sequence, DNA, Complementary genetics, DNA, Complementary metabolism, Fish Diseases microbiology, Fish Proteins chemistry, Fish Proteins metabolism, Gram-Negative Bacterial Infections immunology, Gram-Negative Bacterial Infections microbiology, Interleukin-1beta chemistry, Interleukin-1beta metabolism, Lipopolysaccharides pharmacology, Molecular Sequence Data, Phylogeny, RNA, Messenger genetics, RNA, Messenger metabolism, Real-Time Polymerase Chain Reaction veterinary, Sequence Alignment veterinary, Carps, Fish Diseases immunology, Fish Proteins genetics, Gram-Negative Bacterial Infections veterinary, Interleukin-1beta genetics
- Abstract
Interleukin-1β (IL-1β) is a well-characterized cytokine that plays key roles in cellular responses to infection, inflammation, and immunological challenges in mammals. In this study, we identified and analyzed a grass carp (Ctenopharyngodon idella) ortholog of IL-1β (gcIL-1β), examined its expression patterns in various tissues in both healthy and lipopolysaccharide (LPS)-stimulated specimens, and evaluated its proinflammatory activities. The gcIL-1β gene consists of seven exons and six introns. The full-length cDNA sequence contains an open reading frame of 813 nucleotides. The deduced amino acid sequence exhibits a characteristic IL-1 signature but lacks the typical IL-1β converting enzyme cleavage site that is conserved in mammals. In the phylogenetic tree, IL-1βs from grass carp and other members of the Cyprinidae family clustered into a single group. Expression pattern analysis revealed that gcIL-1β is constitutively expressed in all 11 tissues examined, and LPS stimulation leads to significant up-regulation in muscle, liver, intestine, skin, trunk kidney, head kidney, and gill. Recombinant grass carp IL-1β (rgcIL-1β) was generated prokaryotically as a fusion protein of Trx-rgcIL-1β. An anti-rgcIL-1β polyclonal antibody (rgcIL-1β pAb) was raised in mice against the purified Trx-rgcIL-1β. Western blot analysis confirmed that rgcIL-1β pAb reacted specifically with gcIL-1β in C. idella kidney (CIK) cells. Quantitative real-time PCR data indicated that intestinal mRNA expression levels of endogenous IL-1β, IL-1R2, and TNF-α were significantly up-regulated following Trx-rgcIL-1β exposure. The inhibitory activities of rgcIL-1β pAb against the inflammatory response were confirmed in a model of Aeromonas hydrophila-induced intestinal inflammation. Our immunohistochemical study revealed that the degree and intensity of inflammatory cell infiltration are fully consistent with the observed mRNA expression patterns of these key inflammatory genes. Taken together, these data suggest that gcIL-1β plays a critical role in the proinflammatory response in the grass carp intestine., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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18. Flotation separation of waste plastics for recycling-A review.
- Author
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Wang CQ, Wang H, Fu JG, and Liu YN
- Subjects
- Refuse Disposal, Plastics analysis, Recycling methods, Solid Waste analysis, Waste Management methods
- Abstract
The sharp increase of plastic wastes results in great social and environmental pressures, and recycling, as an effective way currently available to reduce the negative impacts of plastic wastes, represents one of the most dynamic areas in the plastics industry today. Froth flotation is a promising method to solve the key problem of recycling process, namely separation of plastic mixtures. This review surveys recent literature on plastics flotation, focusing on specific features compared to ores flotation, strategies, methods and principles, flotation equipments, and current challenges. In terms of separation methods, plastics flotation is divided into gamma flotation, adsorption of reagents, surface modification and physical regulation., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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19. Separation of polycarbonate and acrylonitrile-butadiene-styrene waste plastics by froth flotation combined with ammonia pretreatment.
- Author
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Wang CQ, Wang H, Liu Q, Fu JG, and Liu YN
- Subjects
- Photoelectron Spectroscopy, Plastics chemistry, Acrylic Resins chemistry, Ammonia chemistry, Butadienes chemistry, Polycarboxylate Cement chemistry, Polystyrenes chemistry, Refuse Disposal methods, Solid Waste analysis
- Abstract
The objective of this research is flotation separation of polycarbonate (PC) and acrylonitrile-butadiene-styrene (ABS) waste plastics combined with ammonia pretreatment. The PC and ABS plastics show similar hydrophobicity, and ammonia treatment changes selectively floatability of PC plastic while ABS is insensitive to ammonia treatment. The contact angle measurement indicates the dropping of flotation recovery of PC is ascribed to a decline of contact angle. X-ray photoelectron spectroscopy demonstrates reactions occur on PC surface, which makes PC surface more hydrophilic. Separation of PC and ABS waste plastics was conducted based on the flotation behavior of single plastic. At different temperatures, PC and ABS mixtures were separated efficiently through froth flotation with ammonia pretreatment for different time (13 min at 23 °C, 18 min at 18 °C and 30 min at 23 °C). For both PC and ABS, the purity and recovery is more than 95.31% and 95.35%, respectively; the purity of PC and ABS is up to 99.72% and 99.23%, respectively. PC and ABS mixtures with different particle sizes were separated effectively, implying that ammonia treatment possesses superior applicability., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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20. Emergence of two novel norovirus genotype II.4 variants associated with viral gastroenteritis in China.
- Author
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Fu JG, Ai J, Qi X, Zhang J, Tang FY, and Zhu YF
- Subjects
- Child, Preschool, China, Cluster Analysis, Female, Genome, Viral, Genotype, Humans, Infant, Infant, Newborn, Male, Molecular Sequence Data, Mutation, Norovirus genetics, Phylogeny, RNA, Viral genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Caliciviridae Infections virology, Gastroenteritis virology, Norovirus classification, Norovirus isolation & purification
- Abstract
Noroviruses (NoVs) are the principal cause of epidemic viral gastroenteritis worldwide, including industrialized and developing countries. Eight hundred and fifty sporadic specimens from hospitalized children with acute gastroenteritis and 131 specimens from seven gastroenteritis outbreaks were collected during 2011-2012 in Jiangsu, China. All specimens were tested for the presence of norovirus (NoV) by real time RT-PCR, and in these, 225/850 of sporadic specimens and 76/131 of outbreak specimens were positive. By sequencing, two novel variants termed JS2011/CHN variant and JS2012/CHN variant were found. By complete genome sequencing and phylogenetic analysis confirmed that both JS2011/CHN variant and JS2012/CHN variant shared more than 98% identity with GII.4 New Orleans/2009/USA strain and GII.4 Sydney/2012/AUS. Both of them had mutations in some key sites in nucleotide sequence and amino acid sequence of ORF1-ORF3. Whether two novel variants will cause epidemic of NoV outbreaks in China deserves further attention. A national surveillance network may be needed to identify trends in molecular evolution of NoVs for prevention of future epidemics., (© 2013 Wiley Periodicals, Inc.)
- Published
- 2014
- Full Text
- View/download PDF
21. Flotability and flotation separation of polymer materials modulated by wetting agents.
- Author
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Wang H, Wang CQ, Fu JG, and Gu GH
- Subjects
- Adsorption, Lignin, Methylcellulose, Octoxynol, Tannins, Materials Testing methods, Polymers chemistry, Refuse Disposal methods, Waste Products analysis, Wetting Agents chemistry
- Abstract
The surface free energy, surface tension and contact angles were performed to investigate the properties of wetting agents. Adsorption of wetting agents changes wetting behavior of polymer resins. Flotability of polymer materials modulated by wetting agents was studied, and wetting agents change significantly flotability of polymer materials. The flotability decreases with increasing the concentration of wetting agents, and the wetting ability is lignin sulfonate (LS)>tannic acid (TA)>methylcellulose (MC)>triton X-100 (TX-100) (from strong to weak). There is significant difference in the flotability between polymer resins and plastics due to the presence of additives in the plastics. Flotation separation of two-component and multicomponent plastics was conducted based on the flotability modulated by wetting agents. The two-component mixtures can be efficiently separated using proper wetting agent through simple flotation flowsheet. The multicomponent plastic mixtures can be separated efficiently through multi-stage flotation using TA and LS as wetting agents, and the purity of separated component was above 94%, and the recovery was more than 93%., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
22. Divergent total synthesis of the Lycopodium alkaloids huperzine A, huperzine B, and huperzine U.
- Author
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Ding R, Fu JG, Xu GQ, Sun BF, and Lin GQ
- Subjects
- Alkaloids chemistry, Cyclohexane Monoterpenes, Drugs, Chinese Herbal chemistry, Molecular Structure, Sesquiterpenes chemistry, Stereoisomerism, Alkaloids chemical synthesis, Drugs, Chinese Herbal chemical synthesis, Lycopodium chemistry, Monoterpenes chemistry, Sesquiterpenes chemical synthesis
- Abstract
Huperzine A, huperzine B, and huperzine U are congeners isolated from the Chinese herb Huperzia serrata (= Lycopodium serratum ) in minuscule amounts. The most efficient total synthesis of huperzine A, the first asymmetric total syntheses of huperzine B, and the first total synthesis of huperzine U have been achieved efficiently in overall yields of 17%, 10%, and 9%, respectively, each spanning 10-13 steps from (R)-pulegone. The featured steps include palladium-catalyzed Buchwald-Hartwig coupling and Heck cyclization reactions and an Ir-catalyzed olefin isomerization reaction. This work has established the absolute configurations of huperzine B and huperzine U and revealed that natural huperzine A, huperzine B, and huperzine U possess the same set of absolute stereochemistries, thus providing support for the potential role of huperzine B and huperzine U in the biosynthesis of huperzine A.
- Published
- 2014
- Full Text
- View/download PDF
23. [Molecular features of human bocavirus from infantile diarrhea in Suzhou area, Jiangsu province in 2010-2011].
- Author
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Fu JG, Jiang CL, Qin YF, Ai J, Liu C, Wu B, Qi X, Bao CJ, Zhu YF, and Tang FY
- Subjects
- China epidemiology, Female, Genotype, Human bocavirus isolation & purification, Humans, Infant, Male, Phylogeny, Sequence Analysis, DNA, Diarrhea, Infantile epidemiology, Diarrhea, Infantile virology, Human bocavirus genetics
- Abstract
Objective: To investigate the status of human bocavirus and to identify its epidemiological characteristics as well as genotype distribution in patients with infantile viral diarrhea in Suzhou, Jiangsu province., Methods: 832 fecal specimens from patients with infantile virus diarrhea cases were collected from Suzhou Children's Hospital in 2010-2011. Human bocavirus were detected by Real-Time RT-PCR, and genotype were determined by sequence analysis., Results: Among all the fecal specimens, 51 (6.1%) cases were positive for human bocavirus. The peak season of rotavirus infection was between July and September. Of all the episodes on rotavirus diarrhea, 96% occurred before 2 years of age, with peaks in children with 7-12 months of age. Data from Nucleotide Sequence analysis showed that among 28 samples that carrying HBoV-1, 5 strains belonged to HBoV-2, HBoV type 3 but type 4 were absent., Conclusion: Human bocavirus were detected from fecal specimens of infantile virus diarrhea in Suzhou, with genotype HBoV-1 as the major strain. HBoV-2 genotype was also found.
- Published
- 2013
24. Floatability of polymer materials modulated by frothers.
- Author
-
Wang H, Wang CQ, and Fu JG
- Subjects
- Adsorption, Models, Chemical, Polymers chemistry
- Abstract
Flotation tests of 35 polymer materials were carried out to investigate their floatability modulated by frothers. Results of flotation tests demonstrated that polymer resins and soft PVC showed high floatability, floatability of hard PVC plastics was relatively low and was related to the frothers, and there exists significant difference in the floatability of different post-consumer plastics. Flotation rate of post-consumer plastics varies from 0% to 100%. Furthermore, three-category low-energy surface (LES) was defined based on the hydrophile index of the materials involved in this paper, and an adsorption model was proposed to explain the results of flotation and to discuss the floatability of polymer materials modulated by frothers. Frother molecules are prone to adsorb on the surface of bubble rather than LES at relatively low concentration, bubble adsorbed by frother molecules is prone to approach first-category LES rather than third-category LES, and the structure of liquid film is formed on the first-category LES at large concentration. Floatability of polymer materials modulated by frothers is further discussed: frothers increase the floatability of the first-category LES but decrease the floatability of the third-category LES, while the floatability of the second-category LES is related to the type of frothers., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
25. [Molecular characteristics of acute gastroenteritis outbreaks caused by norovirus, in Jiangsu province].
- Author
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Fu JG, Ai J, Jin M, Liu C, Sha D, Yao P, Wu B, Qi X, Bao CJ, Zhu YF, and Tang FY
- Subjects
- Amino Acid Sequence, Caliciviridae Infections epidemiology, China epidemiology, Disease Outbreaks, Gastroenteritis epidemiology, Genotype, Humans, Phylogeny, RNA, Viral, Caliciviridae Infections virology, Gastroenteritis virology, Norovirus genetics
- Abstract
Objective: To study both the epidemiologic and molecular characteristics of outbreaks caused by norovirus (NoV) with its variants, in Jiangsu., Methods: 67 specimens from seven gastroenteritis outbreaks were collected from October 2012 to March 2013 in Jiangsu. NoV gene group was detected by Real-Time RT-PCR. NoV portions of RdRp gene and VP1 gene were amplified under RT-PCR., Results: Seven gastroenteritis outbreaks were caused by NoV. Among all the fecal specimens,45 (67.2%) showed positive to NoV G II. Study on the genotype was conducted through analyzing the nucleotide sequence of RdRp gene. Based on the RdRp region, 7 strains appeared to be G II, with 3 and 38 strains belonged to G II.4--Sydney variants. Results from phylogenetic analysis confirmed that 38 variants shared 99% identity with G II.4--Sydney. We also amplified the VP1 genes from 6 variants and comparing with 9 epidemic strains on the sequence amino acid sequence. All the strains showed mutation in amino acid sequence at some key sites which were closely related to the forming of neutralizing epitopes., Conclusion: The short interval periods between all 7 NoV outbreaks with identical viral strain indicated the emergence of a new NoV variant in Jiangsu province,that had caused a number of epidemics abroad. Results from our study suggested that the development of monitoring programs on this novel G II.4--Sydney variant should be a part of the NoV surveillance in Jiangsu province or even in the country.
- Published
- 2013
26. [Study on uniformity of optical field in static Fourier transform spectrometer].
- Author
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Lü JG, Liang JQ, Liang ZZ, and Fu JG
- Abstract
Since the Fourier transform spectrometer based on multi-micro mirrors samples the interferogram image which corresponds to each OPD in the transverse optical field, the spatial distribution of the irradiance has much effect on the quality of the interferogram. In the present paper, distribution function is introduced in this spectrometer system. By means of simulation, the contrast of the interferogram modulated by distribution function is depressed in the fringe area and the concomitant line appears in the spectrum. According to theory analysis, the decline of the contrast lies on the distribution function which scans the interferogram periodically. And the concomitant line is the effect of the spectrum shift, which belongs to the modulation function. Finally, the difference image and conversed recovery arithmetic are proposed. Via the simulation, the conversed recovery arithmetic can recover the interferogram and the spectrum evidently.
- Published
- 2012
27. Construction of highly functional quaternary carbon stereocenters via an organocatalytic tandem cyanation-allylic alkylation reaction.
- Author
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Zhuang Z, Pan F, Fu JG, Chen JM, and Liao WW
- Abstract
The first tertiary amine-catalyzed tandem cyanation-allyic alkylation (CAA) reaction of aldehydes, appropriate cyanide sources, and Morita-Baylis-Hillman (MBH) adducts has been developed, which provides a facile access to densely functionalized products containing O-substituted quaternary centers.
- Published
- 2011
- Full Text
- View/download PDF
28. Expression of inflammatory and apoptosis factors following coronary stent implantation in coronary heart disease patients.
- Author
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Liu LL, Lin LR, Lu CX, Fu JG, Chao PL, Jin HW, Zhang ZY, and Yang TC
- Subjects
- Angina, Stable blood, Angina, Stable pathology, Angina, Stable therapy, Angina, Unstable blood, Angina, Unstable pathology, Angina, Unstable therapy, Angioplasty, Balloon, Coronary, Antigens, CD biosynthesis, Antigens, CD blood, Biomarkers blood, Blood Cell Count, Blood Platelets immunology, Blood Platelets pathology, Case-Control Studies, Cell Adhesion immunology, Endothelins biosynthesis, Endothelins immunology, Female, Humans, Immunologic Factors blood, Male, Middle Aged, Monocytes immunology, Monocytes pathology, Neutrophils immunology, Neutrophils pathology, Angina, Stable immunology, Angina, Unstable immunology, Apoptosis immunology, Endothelium, Vascular immunology, Endothelium, Vascular pathology, Immunologic Factors biosynthesis, Stents adverse effects
- Abstract
We investigated the changes in characteristics of neutrophil CD11b, monocyte CD11b, platelet CD62P, endothelin (ET), and neutrophil CD178 in patients with coronary heart disease (CHD) before and after primary coronary stenting. A total of 41 patients with CHD who underwent coronary stenting and 40 control subjects were enrolled in the study. In CHD patients, peripheral blood samples were taken 24 h before and 30 min, 24 h, and 72 h after successful coronary stenting. All markers were significantly elevated in patients with CHD compared with controls (P<0.05). Time-course studies revealed that the expressions of neutrophil CD11b, monocyte CD11b, platelet CD62P, and ET were lower at 30 min post-operation (PO) compared with that at 24 h before operation (BO) (P<0.05). All levels significantly increased from 30 min PO to 24 h PO (P<0.05) and decreased thereafter until 72 h PO (P>0.05). Time course changes in neutrophil CD11b levels after coronary stenting were significantly higher in patients with unstable angina pectoris than in patients with stable angina pectoris (P<0.05). CD11b levels were related to CD62P in patients with CHD (P<0.05). Neutrophil CD11b and monocyte CD11b levels were significantly increased in patients with CHD who underwent coronary stenting compared with controls (P<0.05). Results show that CD11b levels increased, meanwhile, the levels of CD62P and ET increased in CHD patients after coronary stenting. In addition, neutrophil CD178 levels of apoptosis factor in patients, which is important for regression of inflammation, remained high for a period of time after coronary stenting., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
29. Further evaluation of the characteristics of Treponema pallidum-specific IgM antibody in syphilis serofast reaction patients.
- Author
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Lin LR, Zheng WH, Tong ML, Fu ZG, Liu GL, Fu JG, Zhang DW, Yang TC, and Liu LL
- Subjects
- Adult, Age Factors, Antibodies, Bacterial blood, Female, Humans, Immunoglobulin M blood, Male, Middle Aged, Serologic Tests, Sex Factors, Syphilis diagnosis, Treponema pallidum genetics, Young Adult, Antibodies, Bacterial immunology, Immunoglobulin M immunology, Syphilis immunology, Treponema pallidum immunology
- Abstract
Syphilis serofast reaction (SSR) is common in clinical work. From June 2005 to May 2009, 1208 syphilis patients were chosen for research by the Xiamen Center of Clinical Laboratory in China. Serologic tests were performed with toluidine red unheated serum test (TRUST) and Treponema pallidum particle agglutination (TPPA). Then, T. pallidum-specific IgM antibody (TP-IgM) was detected with fluorescent treponemal antibody absorption (FTA-Abs) and TPPA. In this study, patients were divided into the following experimental groups according to the results of TRUST and TPPA: (1) the SSR group consisted of 411 cases with (+) TRUST and (+) TPPA, and without clinical manifestations after 1 year of recommended syphilis treatment; (2) the serum cure group, which was further subdivided into group A consisting of 251cases with (-) TRUST and (+) TPPA; (3) group B consisting of 546 cases with (-) TRUST and (-) TPPA; and (4) the blood donor control group which consisted of 100 cases. We demonstrated that a total of 136 cases (33.09%) of 411 SSR patients were TP-IgM positive by TPPA, and this percentage was markedly higher than that in serum cure group A (9.16%). FTA-Abs analyses revealed similar results. All samples in serum cure group B and the control group were TP-IgM negative, which is identical to our previous report. The present study also indicated that the TP-IgM positive rate was not significantly different among patients with different ages, genders, and clinical phases after 1 year of recommended therapy. From the total of 1208 syphilis patients, 289 were randomly selected for TP-DNA detection by fluorescence quantitative polymerase chain reaction, and the positive rate of TP-DNA was 32.53%, which was slightly higher than that of FTA-Abs TP-IgM, and no statistically significant difference by chi-square tests, indicating the TP-DNA result is preferably consistent with FTA-Abs and supporting our deduction that TP-IgM could be used as a serologic marker for the relapse and infection of syphilis., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
30. [Analysis of collimation error in Fourier transform infrared spectrometer based on step mirrors].
- Author
-
Lü JG, Liang ZZ, Fu JG, and Liang JQ
- Abstract
The collimation error in Fourier transform spectrometer has many effects on the interferogram and the spectrogram. By means of modulation, the authors found the reverse of contrast in the interferogram and the side slope noise in the spectrogram due to the collimation error. The authors analyzed the signal-to-noise ratio and resolution at different factor of beam divergence. When the factor of the beam divergence is 0.15 degrees x mm(-1), the signal-to-noise ratio can reduce to 6 dB and the resolution can go bad to 13.4 cm(-1).
- Published
- 2011
31. [Influence of extended light source on spectral reconstruction in a micro FTIR].
- Author
-
Fu JG, Liang ZZ, and Liang JQ
- Abstract
The influence of extended light source on spectral reconstruction in the FTIR (fourier transform infrared spectrometer) based on step mirrors was discussed in the present paper. The relationship between coherent intensity and solid angle was calculated. It was found that the coherent length became shorter and the spectrum resolving power became lower as a result of solid angle produced by the extended light source. The spectrum resolving power of system could reach the value as the theoretic design if the solid angle of extended light source is smaller than 0.001. The radiance will reduce if the size of extended light source is shortened. Therefore, a suitable size of extended light source can be chosen, considering the requirement of SNR in the optical design.
- Published
- 2011
32. [Analysis of diffraction in Fourier-transform infrared spectrometer based on step mirrors].
- Author
-
Fu JG, Liang ZZ, and Liang JQ
- Abstract
Diffraction in the Fourier-transform infrared spectrometer based on step mirrors was analyzed in the present paper, and the influence of diffraction on the spectrum reconstruction with different width of step mirrors, wavelengths and distance was also discussed. It is shown that the influence of diffraction is low when the distance is less than 10 cm and the width of mirror is more than 0.5 mm. Also, a method to reduce the noises in spectrum reconstuction is discussed in this paper. The results could be used in the design of the spectrometer and the data processing in the next step.
- Published
- 2010
33. [Serologic survey on pandemic influenza A (H1N1) virus among aged ≥ 3 years population from Jiangsu province in 2009, China].
- Author
-
Tang FY, Zu RQ, Li L, Qi X, Ji H, Wu B, Xu K, Fu JG, Qin YF, Deng F, and Yang HT
- Subjects
- Adolescent, Adult, Aged, Antibodies, Viral blood, Child, China epidemiology, Female, Humans, Influenza A Virus, H1N1 Subtype, Influenza, Human blood, Influenza, Human virology, Male, Middle Aged, Seroepidemiologic Studies, Young Adult, Influenza, Human epidemiology
- Abstract
Objective: To understand the antibody levels against pandemic influenza A (H1N1) virus (2009 H1N1) among aged ≥ 3 years population in 2009, from Jiangsu province, and to describe the distribution of 2009 H1N1., Methods: Serum specimens were collected from natural populations at four different periods in Jiangsu, and tested with hemagglutination-inhibition (HI) assays. Rates of protective antibody against 2009 H1N1 and Geometric mean titers (GMTs) were estimated., Results: The rates of protective antibody against 2009 H1N1 rose with the progress of epidemics in Jiangsu, which were 3.46%, 7.59%, 16.94%, respectively in July, August and November, 2009. There were no significant differences on the rates of protective antibody between males and females at four different cross-sectional periods (P > 0.05), and no significant differences on GMTs were observed at different periods except for November 2009. Significant differences on rates of protective antibody and GMTs among various age groups were observed at four different periods (P < 0.05), and similar results were observed among different periods in various age groups (P < 0.05). There were significant differences on rates of protective antibody and GMTs among different areas (P < 0.05)., Conclusion: The 2009 H1N1 strain had been widely spread out in Jiangsu province since July 2009. People aged 12 - 17 years became the major victims after August. As of November 2009, the rate of protective antibody against 2009 H1N1 was still low, predicting that the epidemic might continue to exist for a certain period of time.
- Published
- 2010
34. [Preparation and antitumor immunity of long circulating nano-liposome encapsulated tumor specific antigen].
- Author
-
Hu PZ, Zuo JF, Fu JG, Li X, Si SY, Ge W, Zhang XM, and Li ZS
- Subjects
- Animals, Antigens, Neoplasm metabolism, Cancer Vaccines metabolism, Cancer Vaccines pharmacokinetics, Cell Line, Tumor, Cytotoxicity, Immunologic, Female, HSP70 Heat-Shock Proteins metabolism, Interferon-gamma metabolism, Mice, Microscopy, Electron, T-Lymphocytes, Cytotoxic immunology, T-Lymphocytes, Cytotoxic metabolism, T-Lymphocytes, Cytotoxic ultrastructure, Time Factors, Antigens, Neoplasm blood, Antigens, Neoplasm immunology, Cancer Vaccines blood, Cancer Vaccines immunology, Liposomes chemistry, Nanostructures chemistry
- Abstract
Aim: To prepare Nano-Liposome encapsulated MAGE3/HSP70(NL M3H) and study its character and antitumor immunity in mouse., Methods: NL M3H was prepared by the thin film-dispersion ultrasonic. The shape and size of NL M3H were detected by electron microscope. The encapsulation rate, drug-carrying capacity, stability and the releasing character were tested by Sephedex-G100 gel filtration. The mouse was immunized by NL M3H, and the antitumor immunity was detected by ELISPOT and LDH release assay., Results: The mean size of NL M3H was lower than 100 nm. The encapsulation rate was 38%.The drug content was 0.038 g/L. NL M3H has good stability after stored in 4 degrees C for 6 months. The releasing profile showed that 74 percent of proteins was released during the first 24 hours in saline. The results of ELISPOT and LDH release assay showed that NL M3H generated tumor specific cytotoxic T lymphocyte(CTL)to damage tumor cell., Conclusion: NL M3H has novel characters, it can generate specific CTL to kill tumor cell, and can be used as new kind of vaccine against tumor.
- Published
- 2009
35. [Property of liposomal fusion induced by acid-sensitive polymer].
- Author
-
Wang RT, Chen T, Wang Z, Hui MQ, and Fu JG
- Subjects
- Alkylation, Drug Carriers, Erythrocyte Membrane metabolism, Humans, Hydrogen-Ion Concentration, Lipids chemistry, Membrane Fusion, Molecular Weight, Particle Size, Polymers, Temperature, Acrylates chemistry, Drug Delivery Systems, Liposomes chemistry
- Abstract
The fusion between liposome-liposome, liposome-biomembarnes induced by acid-sensitive polymers has been systematically investigated. The polymer-liposomes were constructed by post-insertion method with the poly (2-ethylacrylic acid) (PEAA) alkylamide derivatives. The liposomal fusion was studied by use of fluorescence resonance energy transfer assay, particle size, fluorescent-photometer. The results indicated that the poly (2-ethylacrylic acid)-liposomes has very strong acidic induced fusion capability. Under acidic conditions, acid-sensitive polymer liposomes fused each other, the fusion closely related to the molecular weight of acid sensitivity polymer on the surface of liposomes. The acidic fusion of polymer-liposomes was dependent upon the lipids composition, the degree of fusion was reversely related to the cholesterol contents. Acid-en ci-nsitive polymer liposomes fused with erythrocyte ghosts. The liposomal fusion induced by acid-sensitive polymer associated with the increase of membrane permeability. The good acid-sensitivity of PEAA has been further demonstrated by membrane fusion in current experiments, and the liposomes prepared with lipid anchored-poly (2-ethylacrylic acid) were developeds s a potential pH sensitive delivery system.
- Published
- 2008
36. Screen p53 mutations in hepatocellular carcinoma by FASAY: a novel splicing mutation.
- Author
-
Wu XM, Fu JG, Ge WZ, Zhu JY, Wang JY, Zhang W, Qian W, and Huo KK
- Subjects
- Gene Frequency, Genetic Testing, Humans, Mutation, Reproducibility of Results, Sensitivity and Specificity, Alternative Splicing genetics, Carcinoma, Hepatocellular genetics, DNA Mutational Analysis methods, Liver Neoplasms genetics, Tumor Suppressor Protein p53 genetics
- Abstract
Objective: To establish a routine procedure for the detection of p53 mutations in hepatocellular carcinoma (HCC) surgical resections using the FASAY (functional analysis of separated alleles of p53 on yeast) procedure., Methods: p53 status was analyzed by FASAY and cDNA sequencing in 50 cases of HCC. After the extraction of RNA from the frozen tumor and corresponding normal tissues, reverse transcription RT-PCR was carried out using these samples. The assay can detect mutations of p53 mRNA between codons 67 and 347 by the DNA-binding activity of the protein and reveal them as red colonies., Results: Of the 50 specimens, 29 (58%) were positive (mutant) by FASAY. Sequencing analysis confirmed that all 29 FASAY positive tumors harbored mutations, and that no mutations were detectable in any FASAY negative tumors. In 29 p53 mutations, 22 mutations were point missense mutation, 5 were deletions and 2 were splicing mutations. A novel splice mutation on splice donor of intron 6 was reported, which could produce two different mRNAs, respectively using the nearest upstream and downstream recessive splice donor sites., Conclusion: FASAY is a sensitive method for detecting the various types of p53 mutations in HCC, suggesting that the yeast functional assay for the detection of p53 mutations may be essential for elucidating their clinical significance.
- Published
- 2007
- Full Text
- View/download PDF
37. [Therapeutic efficacy of oxymatrine on arrhythmia and heart rate variability in patients with coronary heart disease].
- Author
-
Guo ZB, Fu JG, and Zhao Y
- Subjects
- Adult, Aged, Anti-Arrhythmia Agents therapeutic use, Arrhythmias, Cardiac physiopathology, Coronary Artery Disease physiopathology, Drugs, Chinese Herbal therapeutic use, Female, Humans, Male, Middle Aged, Phytotherapy, Treatment Outcome, Alkaloids therapeutic use, Arrhythmias, Cardiac drug therapy, Coronary Artery Disease drug therapy, Heart Rate drug effects, Quinolizines therapeutic use
- Abstract
Objective: To evaluate the therapeutic efficacy of oxymatrine (Oxy) on arrhythmia and heart rate variability (HRV) in patients with coronary heart disease (CHD) by Holter electrocardiogram and HRV indexes., Methods: One hundred and ten patients with CHD and frequent arrhythmia were randomly divided into the Oxy group and the propafenone group, 55 cases in each group. Changes of general conditions, 48 hrs Holter electrocardiogram, frequency of premature beats, P-R interval, R-R interval and 24 hrs HRV index in patients before and after treatment were evaluated., Results: The amount of both atrial and ventricular premature beats decreased markedly in both groups (P < 0.01). Propafenone showed better effect in reducing atrial premature beats than that of Oxy (P < 0.05), while the two had similar effect on ventricular premature beats (P > 0.05). HRV was elevated in both groups after treatment (P < 0.01), but the effect of Oxy was superior to that of propafenone after treatment (P < 0.05)., Conclusion: Oxymatrine could significantly improve HRV, and shows obvious effects on atrial and ventricular arrhythmia in patients with CHD.
- Published
- 2006
38. [Progress of cardiovascular pharmacologic study on berbamine].
- Author
-
Guo ZB and Fu JG
- Subjects
- Heart Rate drug effects, Ion Channel Gating drug effects, Platelet Aggregation, Alkaloids pharmacology, Anti-Arrhythmia Agents pharmacology, Antihypertensive Agents pharmacology, Benzylisoquinolines pharmacology, Platelet Aggregation Inhibitors pharmacology
- Abstract
Berbamine (molecular formular C37H40N2O6) is a bi-benzle-isoquinolyl alkaloid extracted from Berberis poiretil Schneid (genus of Berberis, family of Beridaceae), a kind of Chinese plants. In aspect of cardiovascular pharmacology, berbamine shows actions of anti-arrhythmia, anti-myocardial ischemia, vasodilatating to lower blood pressure, and antithrombosis, it could lower heart function and heart rate. Study on its anti-arrhythmia was the deepest one. The significant anti-arrhythmia action can be achieved by inhibiting ionic channels of sodium, potassium, calcium, etc., negative frequency and negative transduction, improving the diastolic excitation threshold of myocardium, prolonging effective refractory period of myocardium. As a direction of researches on new type of antiarrhythmic herbs and herbal drugs, the study on berbamine is worthy of further research and development.
- Published
- 2005
39. [Influence of benign prostatic hyperplasia therapy on patients' quality of life].
- Author
-
Fu JG and Sun ZQ
- Subjects
- Humans, Male, Prostatic Hyperplasia psychology, Prostatic Hyperplasia therapy, Quality of Life
- Published
- 2004
40. [Development and application of geographic information system of Aedes vector in Chaozhou city, Guangdong province].
- Author
-
Yi BT, Xu DZ, Zhang ZY, Zhang B, Xi YZ, Fu JG, Luo J, Yuan MH, and Liu SQ
- Subjects
- Animals, China, Dengue prevention & control, Dengue Virus, Ecology, Geographic Information Systems standards, Geography, Insect Vectors virology, Aedes, Geographic Information Systems statistics & numerical data
- Abstract
Objective: To explore the spatial distribution character of dengue fever and the change of Aedes' population, so as to provide macroscopical decision-making evidences of prevention and supervision on dengue fever., Methods: (1) Collecting data on morbidity of dengue and supervision on vector's population in the corresponding period. (2) Drawing digitized map of Chaozhou in scale of 1:50,000, including elements of boundary, residential areas, road and traffic, altitude, water systems etc. (3) Measuring the latitude and longitude of center position of surveillance safes on the scene. (4) Processing spatial analysis by the ArcGIS 8.5 software., Results: Distribution of Aedes showed spatial cluster in Chaozhou, while its density was related to the distance to the watersides. The closer to the watersides, the higher the density was. Map on spatial distribution showed that although the Aedes epidemic situation changed yearly, but primarily be kept in high, middle, low regions. Cross-validation effects of the distribution maps were satisfactory., Conclusion: Geographic information system was promising in analyzing data on dengue fever, and better than other routine research methods.
- Published
- 2004
41. Chinese bittersweet alkaloid III, a new compound from Celastrus angulatus.
- Author
-
Yin WP, Zhao TZ, and Fu JG
- Subjects
- Alkaloids chemistry, Drugs, Chinese Herbal chemistry, Mass Spectrometry, Molecular Conformation, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Piperazines chemistry, Piperidines chemistry, Plant Leaves chemistry, Plants, Medicinal chemistry, Seeds chemistry, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Stereoisomerism, Alkaloids isolation & purification, Celastraceae chemistry, Drugs, Chinese Herbal isolation & purification, Piperazines isolation & purification, Piperidines isolation & purification
- Abstract
A novel skeleton alkaloid named Chinese Bittersweet Alkaloid III from the leaves of C. angulatus was reported. The structure and its stereochemistry were established by IR, ID and 2DNMR (1H-1HCOSY, HMQC, HMBC, NOESY), MS and elemental analysis.
- Published
- 2001
- Full Text
- View/download PDF
42. [The proper time of spraying the leaves of Coix lacrymajobi with phosphorus].
- Author
-
Fu JG
- Subjects
- Fertilizers, Time Factors, Medicine, Chinese Traditional, Medicine, East Asian Traditional, Phosphorus, Plants, Medicinal growth & development
- Published
- 1986
43. [Elements of micro-amounts in the roots of Panax quinquefolium L].
- Author
-
Liu TC, Liu HQ, Zhao YJ, Fu JG, and Hu BY
- Subjects
- China, United States, Panax analysis, Plants, Medicinal, Trace Elements analysis
- Published
- 1987
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