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Indoor air quality is crucial for human health due to the significant time people spend at home, and it is mainly affected by internal sources such as solid fuel combustion for heating. This study investigated the indoor air quality and health implications associated with residential coal burning covering gaseous pollutants (CO, CO 2 and total volatile organic compounds), particulate matter, and toxicity. The PM 10 chemical composition was obtained by ICP-MS/OES (elements), ion chromatography (water-soluble ions) and thermal-optical analysis (organic and elemental carbon). During coal combustion, PM 10 levels were higher (up to 8.8 times) than background levels and the indoor-to-outdoor ratios were, on average, greater than unity, confirming the existence of a significant indoor source. The chemical characterisation of PM 10 revealed increased concentrations of organic carbon and elemental carbon during coal combustion as well as arsenic, cadmium and lead. Carcinogenic risks associated with exposure to arsenic exceeded safety thresholds. Indoor air quality fluctuated during the study, with varying toxicity levels assessed using the Aliivibrio fischeri bioluminescence inhibition assay. These findings underscore the importance of mitigating indoor air pollution associated with coal burning and highlight the potential health risks from long-term exposure. Effective interventions are needed to improve indoor air quality and reduce health risks in coal-burning households., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Fungal spores, commonly found in the atmosphere, can trigger important respiratory disorders. The glycoprotein Alt a 1 is the major allergen present in conidia of the genus Alternaria and has a high clinical relevance for people sensitized to fungi. Exposure to this allergen has been traditionally assessed by aerobiological spore counts, although this does not always offer an accurate estimate of airborne allergen load. This study aims to pinpoint the key factors that explain the presence and variation of Alt a 1 concentration in the atmosphere in order to establish exposure risk periods and improve forecasting models. Alternaria spores were sampled using a Hirst-type volumetric sampler over a five-year period. The allergenic fraction from the bioaerosol was collected using a low-volume cyclone sampler and Alt a 1 quantified by Enzyme-Linked ImmunoSorbent Assay. A cluster analysis was executed in order to group days with similar environmental features and then analyze days with the presence of the allergen in each of them. Subsequently, a quadratic discriminant analysis was performed to evaluate if the selected variables can predict days with high Alt a 1 load. The results indicate that higher temperatures and absolute humidity favor the presence of Alt a 1 in the atmosphere, while time of precipitation is related to days without allergen. Moreover, using the selected parameters, the quadratic discriminant analysis to predict days with allergen showed an accuracy rate between 67 % and 85 %. The mismatch between daily airborne concentration of Alternaria spores and allergen load can be explained by the greater contribution of medium-to-long distance transport of the allergen from the major emission sources as compared with spores. Results highlight the importance of conducting aeroallergen quantification studies together with spore counts to improve the forecasting models of allergy risk, especially for fungal spores., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

The below cloud scavenging of aerosols by snow has been analysed in León (NW Spain). Six snow events were registered over the course of one year of study. Ultrafine and accumulation aerosol particles were measured using a scanning mobility particle sizer spectrometer, while hydrometeors were characterized using a disdrometer. Furthermore, the chemical composition of the melted snow-water samples (soluble and insoluble fractions) was analysed. The scavenging coefficient (λ) showed a great variability among events. An effective washing of particles was observed during the first 30 min of snowfall. The mean change in the scavenging efficiency (%ΔC) of particle number concentration (PNC) and λ coefficient during this time interval were: i) nucleation mode: 36.3 % and 3.02 · 10 -4  s -1 ; ii) Aitken mode: 30.4 % and 2.37 · 10 -4  s -1 and iii) accumulation mode: 22.4 % and 1.77 · 10 -4  s -1 . The range of particle sizes that is less efficiently scavenged by snowfall was observed between 400 and 600 nm. When analyzing the whole snow event, an increase of PNC was observed. Two possible explanations underlie this behaviour: it could be caused by changes in air masses or by the resuspension of aerosol particles scavenged by snowflakes upon reaching the ground. A clear relationship was observed between Ca 2+ , SO 4 2- and NO 3 - concentrations of aerosol particles before the snow event and the concentrations registered in the melted snow-water. The largest and smallest changes in aerosol number concentrations were caused by snowflakes of 3 and 6 mm in diameter, respectively. The particle size distributions (PSD) were fitted to log-normal distributions and the parameters were compared before and after snowfall., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Chromatin remodeling is essential to allow full development of alternative gene expression programs in response to environmental changes. In fission yeast, oxidative stress triggers massive transcriptional changes including the activation of hundreds of genes, with the participation of histone modifying complexes and chromatin remodelers. DNA transcription is associated to alterations in DNA topology, and DNA topoisomerases facilitate elongation along gene bodies. Here, we test whether the DNA topoisomerase Top1 participates in the RNA polymerase II-dependent activation of the cellular response to oxidative stress. Cells lacking Top1 are resistant to H2O2 stress. The transcriptome of Δtop1 strain was not greatly affected in the absence of stress, but activation of the anti-stress gene expression program was more sustained than in wild-type cells. Top1 associated to stress open reading frames. While the nucleosomes of stress genes are partially and transiently evicted during stress, the chromatin configuration remains open for longer times in cells lacking Top1, facilitating RNA polymerase II progression. We propose that, by removing DNA tension arising from transcription, Top1 facilitates nucleosome reassembly and works in synergy with the chromatin remodeler Hrp1 as opposing forces to transcription and to Snf22 / Hrp3 opening remodelers., (© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.)

Worldwide coal is still used for household heating purposes not only because it is available and cheap but also due to behavioural issues. Regional variability in fuels and combustion appliances make accurate emission estimates from this source hard to achieve. In the present study, gaseous (CO, VOCs, SO 2 and NO X ) and particulate matter (TSP) emission factors (EFs) were determined for Spanish household coal combustion covering three commercial coals and distinct combustion stages and mimicking usage patterns in real households. TSP samples were analysed to determine water-soluble inorganic ions, metal(loid)s, and organic and elemental carbon (OC and EC). Additionally, the morphology of the emitted particles was also characterised. CO (3.43-169 g kg -1 ), NO X (1.29-6.00 g kg -1 ) and SO 2 (8.96-22.3 g kg -1 ) EFs showed no trend regarding the combustion stage or coal type tested. On the other hand, VOC, TSP and EC EFs were higher for the ignition/devolatilisation combustion stage, regardless of the fuel tested. TSP EFs (0.085-1.08 g kg -1 ) increased with increasing coal volatile matter while the opposite trend was recorded for VOC emissions (0.045-3.39 gC kg -1 ). TSP carbonaceous matter was dominated by EC while OC represented a small fraction of the particulate mass emitted (less than 8 %wt.). Inorganic compounds composed an important fraction of the TSP samples. Sulphate particulate mass fractions (8.66-22.9 %wt.) appeared to increase with coal S-content. Coal combustion released particles with diverse morphologies, including silicate-rich particles, ferro- and glassy-spheres. This study provides novel emission factors to update emission inventories of residential coal combustion. Additionally, detailed chemical profiles were obtained for source apportionment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This study focuses on the analysis of the distribution, both spatial and temporal, of the PM 10 (particulate matter with a diameter of 10 µm or less) concentrations recorded in nine EMEP (European Monitoring and Evaluation Programme) background stations distributed throughout mainland Spain between 2001 and 2019. A study of hierarchical clusters was used to classify the stations into three main groups with similarities in yearly concentrations: GC (coastal location), GNC (north-central location), and GSE (southeastern location). The highest PM 10 concentrations were registered in summer. Annual evolution showed statistically significant decreasing trends in PM 10 concentration in all the stations covering a range from -0.21 to -0.50 µg m -3 /year for Barcarrota and Víznar, respectively. Through the Lamb classification, the weather types were defined during the study period, and those associated with high levels of pollution were identified. Finally, the values exceeding the limits established by the legislation were analyzed for every station assessed in the study.

Pompe disease is an inherited metabolic myopathy caused by deficiency of acid alpha-glucosidase (GAA), resulting in lysosomal glycogen accumulation. Residual GAA enzyme activity affects disease onset and severity, although other factors, including dysregulation of cytoplasmic glycogen metabolism, are suspected to modulate the disease course. In this study, performed in mice and patient biopsies, we found elevated protein levels of enzymes involved in glucose uptake and cytoplasmic glycogen synthesis in skeletal muscle from mice with Pompe disease, including glycogenin (GYG1), glycogen synthase (GYS1), glucose transporter 4 (GLUT4), glycogen branching enzyme 1 (GBE1), and UDP-glucose pyrophosphorylase (UGP2). Expression levels were elevated before the loss of muscle mass and function. For first time, quantitative mass spectrometry in skeletal muscle biopsies from five adult patients with Pompe disease showed increased expression of GBE1 protein relative to healthy controls at the group level. Paired analysis of individual patients who responded well to treatment with enzyme replacement therapy (ERT) showed reduction of GYS1, GYG1, and GBE1 in all patients after start of ERT compared to baseline. These results indicate that metabolic changes precede muscle wasting in Pompe disease, and imply a positive feedforward loop in Pompe disease, in which lysosomal glycogen accumulation promotes cytoplasmic glycogen synthesis and glucose uptake, resulting in aggravation of the disease phenotype., (© 2022 The Authors. Journal of Inherited Metabolic Disease published by John Wiley & Sons Ltd on behalf of SSIEM.)

The MAP kinase Sty1 phosphorylates and activates the transcription factor Atf1 in response to several stress conditions, which then shifts from a transcriptional repressor to an activator. Atf1 also participates in heterochromatin assembly at the mat locus, in combination with the RNA interference (RNAi) machinery. Here, we study the role of signal-dependent phosphorylation of Atf1 in heterochromatin establishment at mat , using different Atf1 phospho mutants. Although a hypo-phosphorylation Atf1 mutant, Atf1.10M, mediates heterochromatin assembly, the phosphomimic Atf1.10D is unable to maintain silencing. In a minimal mat locus, lacking the RNAi-recruiting cis elements and displaying intermediate silencing, Atf1.10M restores full heterochromatin and silencing. However, evolution experiments with this stress-blinded Atf1.10M show that it is unable to facilitate switching between the donor site mat3 and mat1 . We propose that the unphosphorylated, inactive Atf1 contributes to proper heterochromatin assembly by recruiting repressive complexes, but its stress-dependent phosphorylation is required for recombination/switching to occur., Competing Interests: The authors declare no competing interests., (© 2022 The Author(s).)

The atlantoaxial joint C2 (axis) with the anterior arch of C1 (atlas) allows 50% of cervical lateral rotation. It is responsible for precise and important movements that allow us to perform precise actions, both in normal and working life. Due to low incidence in adults, this condition often goes undiagnosed, or the diagnosis is delayed and the outcome is worse. An early diagnosis and treatment are essential to ensure satisfactory neurological and functional outcomes. The aim of this review is to analyze C1-C2 rotatory subluxation in adults, given its rarity. The time between injury and reduction is key, as it is directly related to prognosis and the severity of the treatment options. Due to low incidence in adults, this condition often goes undiagnosed, or the diagnosis is delayed as a lot of cases are not related to a clear trauma, with a poor prognosis just because of the late diagnosis and the outcome is worse. The correct approach and treatment of atlantoaxial dislocation requires a careful study of the radiological findings to decide the direction and plane of the dislocation, and the search for associated skeletal anomalies.

The radiative forcing before and after rain events was studied between 12 February 2016 and 14 March 2017 in León, Spain. For this purpose, the radiative forcing fluxes were calculated using the Radiative Transfer Model Global Atmospheric ModEl (RTM GAME). After the application of a set of selection criteria (based on the availability of AERONET data, rain characteristics and lightning maps), 16 stratiform rain events were identified, concentrated in spring and winter, and 15 convective rain events were found concentrated in spring and summer. Rainfall events were grouped according to the atmospheric forcing (ΔF ATM ) before rain: "low" or "high" (lower or higher than 30 W m -2 ). The threshold has been set at this value because it is the mean ΔF ATM of all the selected events before rain. There were significant statistical differences between stratiform and convective events in rain duration, mean raindrop diameter and parameters a and b of radar reflectivity Z and rainfall intensity R relationship (Z = a R b ). When comparing "low" and "high" groups, raindrop diameter was similar in stratiform (0.51 ± 0.08 vs 0.48 ± 0.12 mm) and convective events (0.96 ± 0.98 vs 0.83 ± 0.63 mm), registering higher values for the latter. In stratiform events, the rain scavenging effect on aerosol particles is clearly observed in the "high" group with a decrease of radiative forcing of -27.0 ± 25.3%, and to a lesser extent, in the "low" group, probably because of a lower aerosol load in the atmosphere. In stratiform events, the mode of the raindrop size gamma distribution presented statistical differences between "low" (0.25 ± 0.13 mm) and "high" (0.35 ± 0.05 mm) groups. We claim that this points towards a relationship between radiative forcing before rain and the specific characteristics of rainfall measured at ground level. This study increases our knowledge on the important role of rainwater as a clean agent of the atmosphere and its impact on climate (through radiative forcing)., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Real-time measurements of particles in the 15-736 nm range have been obtained by a Scanning Mobility Particle Sizer to characterize the evolution of particle size distribution and new particle formation (NPF) events in an urban background area. The annual, weekly and diurnal variations of the modal (nucleation (N nuc ), Aitken (N Ait ) and accumulation (N acc )) particle concentrations were characterised. The N Ait and N acc registered their maximums in cold months during rush hours, in the morning (0600-0900 UTC) and in the afternoon (1700-2000 UTC), while the maximums for N nuc were reached in warm months during midday hours. N Ait , N acc and N total showed a significant negative correlation with wind speed and a different relationship with the planetary boundary layer (PBL) height by periods. In the warm period, a positive significant correlation between PBL and N nuc was registered, indicating that the higher dispersion promoted by a high PBL causes favourable conditions for the occurrence of NPF events (a low polluted atmosphere). NPF processes are one of the main sources of ultrafine particles (<100 nm) in the warm period. After a visual-based classification, 45 NPF events of type Ia (strong and with a good confidence level) were identified and analysed, occurring primarily between 1100 and 1500 UTC, mainly in spring and summer. In addition, a two-step method was developed for identifying NPF events: cluster analysis followed by discriminant analysis. The application of discriminant analysis to one of the clusters, grouping 93 days, enabled us to identify 55 of the 56 NPF events days included in the cluster. This method is a valuable tool for identifying NPF events quickly and effectively., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

This paper studies the below-cloud scavenging caused by precipitation on ultrafine and accumulation modes, as well as the role of the different raindrop sizes in an urban environment. The equipment used to measure aerosol particles and raindrop variables includes a scanning mobility particle sizer spectrometer-SMPS and a Laser Precipitation Monitor (LPM), respectively. An analysis of the scavenging efficiency and the scavenging coefficient (λ) by modes and rain intensities was carried out. The main results observed have been: i) the nucleation (between 14 and 30 nm), Aitken (between 30 and 100 nm), accumulation 1 (between 100 and 300) and accumulation 2 (between 300 and 1000 nm) modes presented a scavenging efficiency of 15, 4, 22 and 21%, respectively; ii) events with rain intensities between 1 and 3 mm h -1 caused less scavenging in all modes; iii) raindrop sizes between 1.25 and 3.5 mm scavenged mainly particle sizes between 70 and 250 nm. Lower scavenging was observed on particle sizes >300 nm, and particle sizes >600 nm were only scavenged by raindrop sizes >4.75 mm; iv) the respirable fraction before and after the rain events presented a statically significant decrease of -35%. The combination in this study of SMPS and disdrometer measurements has resulted in a more detailed characterization of the influence of this process on the submicrometer aerosol fraction, noting that below-cloud scavenging is one of the main removal pathways for submicrometer aerosol particles. This study thus contributes to improving the current state of knowledge of below-cloud scavenging., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Bioaerosols play a major role in the plant life of ecosystems. In addition, they have a profound impact on human health, since they may cause lung diseases or allergies. The key objective of this study is to assess the below cloud scavenging effect of rainfall on pollen concentration. The analysis is based on a sampling carried out in León, Spain, between 2015 and 2018. The rainfall variables and the pollen concentrations have been obtained with a disdrometer and a volumetric Hirst type spore-trap, respectively. In order to evaluate the scavenging, three parameters have been calculated: scavenging efficiency (through the concentration-weighted average (%ΔC)), the scavenging coefficient (λ) and the percentage of events with a decrease in pollen concentration (%ES) also called events with effective scavenging. 71% of rain events presented an effective scavenging that affected all types of pollen. The %ΔC mean value of total pollen was 24 ± 18% (positive values indicate an effective scavenging) and the types of pollen with the highest values were Castanea and Cupressaceae (71 and 40%, respectively). A linear model (R 2  = 0.94) to estimate the pollen concentration after rain was built with variables such as pollen concentration before rain and other variables from a weather station and a disdrometer. Furthermore, we have shown the possibility of knowing in real time the probable Cupressaceae pollen concentration, from the initial pollen concentration and the physical parameters of rain (such as raindrop size, rain intensity or volume swept by raindrops in their falling path)., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation., Competing Interests: The authors declare no competing interests., (© 2021 The Author(s).)

A one-year study was carried out in León, Spain, in order to characterize physically and chemically the precipitation. With the aim of studying the scavenging process of atmospheric pollutants, scavenging ratio and removal coefficients were calculated through physical parameters of raindrops (obtained by disdrometer data) and through chemical properties of aerosols. Finally, linear models for the prediction of the chemical composition of rainwater and the efficiency of the removal effect were established. In general, the rainwater was dominated by NH 4 + > SO 4 2- > NO 3 - in all seasons. Higher ion concentrations and conductivity and lowest pH were observed in summer, due to the low volume of rain. In winter, the high values of Na + and Cl - in the rainwater showed the contribution from marine sources, while in summer the high concentrations of Ca 2+ , Mg 2+ , SO 4 2- , NH 4 + and NO 3 - reflected the contribution from both crustal and anthropogenic sources. The linear models revealed that the amount of dissolved organic carbon and of the water-soluble ions in rain samples, Ca 2+ , SO 4 2- , NO 3 - , increases with the volume swept by the falling drops. Insoluble carbon fraction has a negative dependence with the volume swept and positive with the diameter of the raindrop. Removal coefficients are affected by the concentration in the air of each species before precipitation, the duration of the event and the time elapsed between two precipitation events., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier Ltd.)

A one-year aerosol sampling campaign, between 2016 and 2017, was conducted in a suburban area of León city, Spain. An association between the Positive Matrix Factorization (PMF) results and air masses through circulation weather types was carried out, through the construction of linear models from the PM 10 concentrations and its chemical composition. The aerosol sources, identified by PMF six-factor solution, were: traffic (29%), aged sea salt (26%), secondary aerosols (16%), dust (13%), marine aerosol (7%) and biomass burning (3%). Traffic and secondary factors showed the highest PM 10 contribution in the hybrid cyclonic types with wind component from the first and second quadrant. Anticyclonic types with wind component from the first quadrant exhibited high values of secondary, aged sea salt and dust factors. The highest contributions of the dust factor were also associated with northerly types. The linear models built for estimating the source apportionment of PM 10 , from aerosol chemical composition and geostrophic flow, showed positive coefficients for: westerly flows (WF) in marine factor, southerly flows (SF) in secondary and traffic factors, and shear southerly vorticities (ZS) in dust factor. Negative dependences were observed for ZS in aged sea salt factor and for SF in dust factor. The PM 10 mass concentration calculated by the linear models and by the PMF model were strongly correlated. This can be very useful to determine the contribution of a specific source to PM 10 in León, only by knowing some meteorological and chemical variables., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

A one-year campaign of joint sampling of aerosols and precipitation, carried out in León, Spain, allowed to study the impact of two special events that affected the air quality in the north of the country, on rainfall in the city: a period with wildfires and a Saharan dust intrusion. The wildfires that occurred in northern Portugal and northwestern Spain in August 2016 affected the chemistry of rainfall on 15 August 2016, causing an increase in concentrations of NH 4 + , Na + , Cl - , K + , Mg 2+ , Ca 2+ , SO 4 2- and NO 3 - and in the concentrations of organic acids, which was reflected in the levels of soluble and insoluble organic carbon. This led to acidification of rainwater (pH = 4.8). The second precipitation event was registered between 11 and 14 February 2017, during which the rainwater was collected in four daily fractions (P 1 , P 2 , P 3 and P 4 ). The rain sample of 12 February (P 2 ) coincided with a Saharan dust intrusion that reached northern Iberia that day. The chemical composition of P 2 showed an increase in the Ca 2+ (>800%), Mg 2+ (71%), Cl - (62%), and SO 4 2- (33%) concentrations, with respect to P 1 . The input of crustal elements to the atmosphere helped to neutralize the P 2 rainwater, causing pH values higher than 6.5. Once the dust intrusion left the north of the Peninsula, the composition of rainwater P 3 and P 4 revealed a mixture of marine contribution with local anthropogenic emissions, as well as a decrease in ion concentrations and conductivity, and an increase in pH values., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Transcription factors are often the downstream effectors of signaling cascades. In fission yeast, the transcription factor Atf1 is phosphorylated by the MAP kinase Sty1 under several environmental stressors to promote transcription initiation of stress genes. However, Sty1 and Atf1 have also been involved in other cellular processes such as homologous recombination at hotspots, ste11 gene expression during mating and meiosis, or regulation of fbp1 gene transcription under glucose starvation conditions. Using different phospho-mutants of Atf1, we have investigated the role of Atf1 phosphorylation by Sty1 in those biological processes. An Atf1 mutant lacking the canonical MAP kinase phosphorylation sites cannot activate fbp1 transcription when glucose is depleted, but it is still able to induce recombination at ade6.M26 and to induce ste11 after nitrogen depletion; in these last cases, Sty1 is still required, suggesting that additional non-canonical sites are activating the transcription factor. In all cases, an Atf1 phosphomimetic mutant bypasses the requirement of the Sty1 kinase in these diverse biological processes, highlighting the essential role of the DNA binding factor Atf1 on chromatin remodeling and cell adaptation to nutritional changes. We propose that post-translational modifications of Atf1 by Sty1, either at canonical or non-canonical sites, are sufficient to activate some of the functions of Atf1, those involving chromatin remodeling and transcription initiation. However, in the case of fbp1 where Atf1 acts synergistically with other transcription factors, elimination of the canonical sites is sufficient to hamper some of the interactions required in this complex scenario and to impair transcription initiation., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Background: External fixator knee arthrodesis is a salvage procedure used primarily in cases of end-stage infected total knee replacement (iTKR). Stable fixation combined with bone-end compression is essential to achieve knee fusion, but providing sufficient stability can be challenging in the presence of severe bone loss. Our hypothesis is that using an external fixation biplanar configuration would bring about a fusion rate superior to that of a monolateral frame., Methods: This study compares outcomes of biplanar external fixator knee fusion due to non-revisable iTKR with those of a historical cohort control study with patients managed with a monoplanar configuration. Primary endpoints were fusion rate, time to achieve bone fusion and infection eradication rate. Limb-length discrepancy, pain level, patient satisfaction and health-related quality of life were evaluated., Results: A total of 29 knee fusion cases were included. In the biplanar group, infection was eradicated in 100% of the patients and fusion was achieved in all cases within an average of 5.24 months. In comparison, in the monolateral group, infection was eradicated in 86% of the cases and fusion was achieved in 81% of the patients after a mean of 10.3 months (p < 0.05). In both groups, postoperative pain was mild and patients expressed a high degree of satisfaction once fusion was achieved., Conclusions: According to our data, external fixation knee fusion is a useful limb-salvage procedure in end-stage cases of knee PJI. We conclude that a biplanar configuration can halve the time required to achieve solid bone fusion in such a complex scenario.

Survival upon glucose starvation requires a delicate balance between different metabolic pathways. A recent work by the Roe laboratory provides a mechanistic link between glucose deprivation and the regulation of the pentose phosphate pathway, with the transcriptional repressor Rsv1 playing a key role in the process. Rsv1 regulates the flow of glucose into its possible metabolic fates and promotes long-term survival under low glucose., (© 2019 Federation of European Biochemical Societies.)

A sampling campaign was conducted in the Liberdade Avenue tunnel (Braga, Portugal) during a week (with 56,000 vehicles) to monitor black carbon (eBC-equivalent black carbon) by means of an Aethalometer AE-31, and gaseous pollutants (CO 2 , CO, NO x ). Inside the tunnel, the mean eBC mass concentration was 21 ± 10 μg m - 3 . An hourly and weekday-weekend study was carried out. Regarding the Absorption Ångström exponent (AAE), a mean value of 0.97 ± 0.10 was obtained, for a source of practically pure traffic. There was a positive significant correlation between eBC and the number of light vehicles (r = 0.47; p < 0.001) and between eBC and the gaseous emissions: CO (r = 0.67; p < 0.001), CO - (r = 0.71; p < 0.001), NO (r = 0.63; p < 0.001) and NO 3 (r = 0.70; p < 0.001). The mean black carbon emission factors (EF 2 (r = 0.71; p < 0.001), NO (r = 0.63; p < 0.001) and NO 2 (r = 0.70; p < 0.001). The mean black carbon emission factors (EF BC ) inside the tunnel were 0.31 ± 0.08 g (kg fuel) - 1 and 0.11 ± 0.08 mg veh - 1  km - 1 , similar to those found in other studies for gasoline and diesel vehicles in road tunnels., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Skeletal muscle is capable of efficiently regenerating after damage in a process mediated by tissue-resident stem cells called satellite cells. This regenerative potential is often compromised under muscle-degenerative conditions. Consequently, the damage produced during degeneration is not efficiently repaired and the balance between repair and damage is lost. Here we review recent progress on the role of satellite cell-mediated repair in neuromuscular disorders with a focus on Pompe disease, an inherited metabolic myopathy caused by deficiency of the lysosomal enzyme acid alpha glucosidase (GAA). Studies performed in patient biopsies as well as in Pompe disease mouse models demonstrate that muscle regeneration activity is compromised despite progressing muscle damage. We describe disease-specific mechanisms of satellite cell dysfunction to highlight the differences between Pompe disease and muscle dystrophies. The mechanisms involved provide possible targets for therapy, such as modulation of autophagy, muscle exercise, and pharmacological modulation of satellite cell activation. Most of these approaches are still experimental, although promising in animal models, still warrant caution with respect to their safety and efficiency profile., Competing Interests: Conflicts of Interest: The authors have no conflicts of interest to declare.

Saharan air masses can transport high amounts of mineral dust particles and biological material to the Iberian Peninsula. During winter, this kind of events is not very frequent and usually does not reach the northwest of the Peninsula. However, between 21 and 22 February 2016 and between 22 and 23 February 2017, two exceptional events were registered in León (Spain), which severely affected air quality. An integrative approach including: i) typical synoptic conditions; ii) aerosol chemical composition; iii) particle size distributions; iv) pollen concentration; v) aerosol optical depth (AOD); vi) radiative forcing and vii) estimation of the impact of aerosols in the respiratory tract, was carried out. In the global characterization of these events, the exceedance of the PM 10 daily limit value, an increase in the coarse mode and a rise in the iron concentration were observed. On the 2016 event, an AOD and extinction-related Ångström exponent clearly characteristic of desert aerosol (1.1 and 0.05, respectively) were registered. Furthermore, pollen grains not typical of flowering plants in this period were identified. The chemical analysis of the aerosol from the 2017 event allowed us to confirm the presence of the main elements associated with mineral sources (aluminum, calcium, and silica concentrations). An increase in the SO 4 2- , NO 3 - and Cl - concentrations during the Saharan dust intrusion was also noted. However, in this event, there was no presence of atypical pollen types. The estimated dust radiative forcing traduced a cooling effect for surface and atmosphere during both events, corroborated by trends of radiative flux measurements. The estimated impact on the respiratory tract regions of the high levels of particulate matter during both Saharan dust intrusions showed high levels for the respirable fraction., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Aerosol black carbon (BC) is the second strongest contributor to global warming, after CO 2 , and it is linked to many adverse health effects. A sampling campaign of 15 months was carried out in León (Spain) in order to evaluate the scavenging of BC with an ensemble aethalometer-disdrometer. The aethalometer provides the concentration of equivalent black carbon (eBC), and the disdrometer, the raindrop size distribution. A total of seventy-five rain events were studied and in 73% of them there was an effective (eBC initial  > eBC final ) scavenging, with a mean decrease of 48 ± 37% in long rain events (>8 h) and 39 ± 38% in short rain events. The scavenging of BC is strongly related to its source. Thus, the scavenging coefficient (SC) mean value of the BC from fossil fuel (eBC ff ) for short and long rain events was 5.1 10 -5 and 1.3 10 -5 s -1 , respectively. For the BC from biomass burning (eBC bb ), the SC values were 1.6 10 -4 and 2.8 10 -5 s -1 in short and long events, respectively. There was a significant positive correlation between the SC and the number of drops with diameters between 0.375 and 2.5 mm. Rain scavenging of eBC was analyzed depending on the air mass origin obtaining an effective scavenging for air masses from Atlantic, Arctic and Africa. A linear model (R 2  = 0.72) was built to estimate the ΔeBC values with variables from an aethalometer, a disdrometer and a weather station: eBC concentration before rain, swept volume and precipitation accumulated. A Kolmogorov-Smirnov statistical test confirmed the goodness of fit of the model to the measured data., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Aims: The poor control of symptoms in patients with advanced heart failure with reduced ejection function (HFrEF) can limit the functionality of patients. Sacubitril-valsartan, compared with enalapril, has been shown to reduce mortality and hospitalization, and nowadays, there is still little evidence about the improvement on functionality. The aim of our study is to analyse the improvement of the functional class and the 6 min walking test (6MWT) in patients with multiple pathologies and advanced heart failure., Methods and Results: From September 2016 to March 2018, 65 multimorbidity patients with severe symptomatic HFrEF were initiated to receive sacubitril-valsartan. Mean age was 78.6 ± 7.4 years, and 68% were male. The Charlson co-morbidity index was 8 points. Seventy-four per cent had New York Heart Association (NYHA) Functional Class IV. After the treatment, patients were able to achieve 55.68 m or more on 6MWT, and 91% presented an improvement in the NYHA functional class., Conclusions: Sacubitril-valsartan relieves symptoms and improves functional class prognostic risk of patients with advanced HFrEF and co-morbidity., (© 2018 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology.)

Presently, both in rural areas and in cities open fireplaces are still present and large quantities of wood are combusted every year. The present study aims to characterize aerosol size distribution, chemical composition and deposition in the human respiratory tract of particles emitted during the combustion of logs of oak in an open fireplace installed in the living room of a typical village house. CO 2 and CO levels and aerosol size distribution have been continuously monitored and a PM 10 sampler with two types of filters for chemical and microscopic analysis was also installed. The increment, between the operating periods and the indoor background, in the organic carbon and PM 10 concentration due to the use of the fireplace is 15.7±0.6 (mean±standard deviation) and 58.5±6.2μgm -3 , respectively. The two main polluting processes during the operation of the fireplace are the ignition with the subsequent refueling and the final cleaning of the residual ashes. In both phases mean values around 1800 particles cm -3 with CMD of 0.15μm were measured. However, while PM 10 levels of 130±120μgm -3 were estimated for the ignition stage, values of 200±200μgm -3 were obtained during the final cleaning step. Assessment conducted according to ISO standard 7708:1995, demonstrated that a person who stays in a living room when an open fireplace is lit will inhale, on average, 217μgm -3 and 283μgm -3 during the ignition and the refueling stages, respectively. Subsequent refueling proved to be much less polluting. The ashes removal can also be very polluting and dangerous to health if there are hidden small incandescent embers among the ashes (estimated PM 10 of 132μgm -3 ), reaching a CO 2 level of 1940ppm and a dangerous level of CO of 132ppm., (Copyright © 2018 Elsevier B.V. All rights reserved.)

This study provides an analysis of the spatial distribution and trends of NO, NO2 and O3 concentrations in Portugal between 1995 and 2010. Furthermore, an estimation model for daily ozone concentrations was developed for an urban and a rural site. NO concentration showed a significant decreasing trend in most urban stations. A decreasing trend in NO2 is only observed in the stations with less influence from emissions of primary NO2. Several stations showed a significant upward trend in O3 as a result of the decrease in the NO/NO2 ratio. In the northern rural region, ozone showed a strong correlation with wind direction, highlighting the importance of long-range transport. In the urban site, most of the variance is explained by the NO2/NOX ratio. The results obtained by the ozone estimation model in the urban site fit 2013 observed data. In the rural site, the estimated ozone during extreme events agrees with observed concentration.

To date, although much attention has been paid to the estimation and modeling of the voice source (ie, the glottal airflow volume velocity), the measurement and characterization of the supraglottal pressure wave have been much less studied. Some previous results have unveiled that the supraglottal pressure wave has some spectral resonances similar to those of the voice pressure wave. This makes the supraglottal wave partially intelligible. Although the explanation for such effect seems to be clearly related to the reflected pressure wave traveling upstream along the vocal tract, the influence that nonlinear source-filter interaction has on it is not as clear. This article provides an insight into this issue by comparing the acoustic analyses of measured and simulated supraglottal and voice waves. Simulations have been performed using a high-dimensional discrete vocal fold model. Results of such comparative analysis indicate that spectral resonances in the supraglottal wave are mainly caused by the regressive pressure wave that travels upstream along the vocal tract and not by source-tract interaction. On the contrary and according to simulation results, source-tract interaction has a role in the loss of intelligibility that happens in the supraglottal wave with respect to the voice wave. This loss of intelligibility mainly corresponds to spectral differences for frequencies above 1500 Hz., (Copyright © 2016 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Background and Objective: Calciphylaxis is a cutaneous ischaemic vascular disease of small vessels with high morbidity and mortality. To date very few series of patients with this disease have been published, none from a Spanish hospital. The main objectives of this work are to analyze the demographic, clinical and histological profile of patients diagnosed in our department to identify risk factors and potential therapeutic strategies., Material and Method: We made a retrospective study of the cases seen in the dermatology department with a diagnosis of calciphylaxis and who had a confirmatory biopsy in the period between January 2010 to August 2015., Results: Nine patients were studied, with an age range of 76-86 years. All had cardiovascular comorbidities and 67% had renal failure. A 33% mortality was observed., Conclusions: Faced with a possible diagnosis of calciphylaxis, a complete blood analysis is mandatory to rule out other causes of skin ulcers. The management of these patients should be undertaken by a multidisciplinary team. We emphasize the role of sodium thiosulfate in the treatment of this condition., (Copyright © 2016 Elsevier España, S.L.U. All rights reserved.)

This paper presents an electronic system for the automatic detection of hazardous gases. The proposed system implements colorimetric sensing algorithms, thus providing a low-cost solution to the problem of gas sensing. It is remotely operated and it performs the tasks of image capturing and processing, hence obtaining colour measurements in RGB (Red-Green-Blue) space that are subsequently sent to a remote operator via the internet. A prototype of the system has been built to test its performance. Specifically, experiments have been carried out aimed at the detection of CO, CO2, NO, NO2, SO2 and formaldehyde at diverse concentrations by using a chromogenic array composed by 13 active and 2 inert compounds. Statistical analyses of the results reveal a good performance of the electronic system and the feasibility of remote hazardous gas detection using colorimetric sensor arrays., (Copyright © 2015 ISA. Published by Elsevier Ltd. All rights reserved.)

In this study, an indoor/outdoor monitoring program was carried out in a gymnasium at the University of Leon, Spain. The main goal was a characterization of aerosol size distributions in a university gymnasium under different conditions and sports activities (with and without magnesia alba) and the study of the mass fraction deposited in each of the parts of the respiratory tract. The aerosol particles were measured in 31 discrete channels (size ranges) using a laser spectrometer probe. Aerosol size distributions were studied under different conditions: i) before sports activities, ii) activities without using magnesia alba, iii) activities using magnesia alba, iv) cleaning procedures, and v) outdoors. The aerosol refractive index and density indoors were estimated from the aerosol composition: 1.577-0.003i and 2.055 g cm(-3), respectively. Using the estimated density, the mass concentration was calculated, and the evolution of PM1, PM2.5 and PM10 for different activities was assessed. The quality of the air in the gymnasium was strongly influenced by the use of magnesia alba (MgCO3) and the number of gymnasts who were training. Due to the climbing chalk and the constant process of resuspension, average PM10 concentrations of over 440 μg m(-3) were reached. The maximum daily concentrations ranged from 500 to 900 μg m(-3). Particle size determines the place in the respiratory tract where the deposition occurs. For this reason, the inhalable, thoracic, tracheobronchial and respirable fractions were assessed for healthy adults and high risk people, according to international standards. The estimations show that, for healthy adults, up to 300 μg m(-3) can be retained by the trachea and bronchi, and 130 μg m(-3) may reach the alveolar region. The different physical activities and the attendance rates in the sports facility have a significant influence on the concentration and size distributions observed., (Copyright © 2015 Elsevier B.V. All rights reserved.)

An indoor/outdoor monitoring programme of PM10 was carried out in two sports venues (a fronton and a gymnasium). Levels always below 50 μg m(-3) were obtained in the fronton and outdoor air. Due to the climbing chalk and the constant process of resuspension, concentrations above 150 μg m(-3) were registered in the gymnasium. The chalk dust contributed to CO3 (2-) concentrations of 32 ± 9.4 μg m(-3) in this sports facility, which represented, on average, 18 % of the PM10 mass. Here, the carbonate levels were 128 times higher than those registered outdoors. Much lower concentrations, around 1 μg m(-3), were measured in the fronton. The chalk dust is also responsible for the high Mg(2+) concentrations in the gym (4.7 ± 0.89 μg m(-3)), unfolding a PM10 mass fraction of 2.7 %. Total carbon accounted for almost 30 % of PM10 in both indoor spaces. Aerosol size distributions were bimodal and revealed a clear dependence on physical activities and characteristics of the sports facilities. The use of climbing chalk in the gymnasium contributed significantly to the coarse mode. The average geometric mean diameter, geometric standard deviation and total number of coarse particles were 0.77 μm, 2.79 cm(-3) and 28 cm(-3), respectively.

The aim of this study is to improve the estimation of the characteristic uncertainties of optic disdrometers in an attempt to calculate the efficient sampling area according to the size of the drop and to study how this influences the computation of other parameters, taking into account that the real sampling area is always smaller than the nominal area. For large raindrops (a little over 6 mm), the effective sampling area may be half the area indicated by the manufacturer. The error committed in the sampling area is propagated to all the variables depending on this surface, such as the rain intensity and the reflectivity factor. Both variables tend to underestimate the real value if the sampling area is not corrected. For example, the rainfall intensity errors may be up to 50% for large drops, those slightly larger than 6 mm. The same occurs with reflectivity values, which may be up to twice the reflectivity calculated using the uncorrected constant sampling area. The Z-R relationships appear to have little dependence on the sampling area, because both variables depend on it the same way. These results were obtained by studying one particular rain event that occurred on April 16, 2006.

Objectives: This article presents a comparative study of the spectral power distribution for normal and dysphonic voices, both for sustained vowels and running speech. The objective of this study was to find robust cues of dysphonia in spectral domain. For this purpose, recordings from two databases are processed, one of them including both sustained vowels and running speech. Additionally, a new measure of stability is introduced (decorrelation time). The application of this measure to the power spectrum is also tested as a cue of dysphonia., Materials and Methods: The spectral analysis is done having both an auditory model and the filterbank approach as references to the computation of discrete spectrograms. Results are obtained from three sets of recordings belonging to two different databases., Results: The reported results indicate that only minor differences exist in the shape of the power spectrum of normal and dysphonic voices when performing sustained vowel phonation tasks. However, the calculated band power decorrelation times indicate that power in bands between 2000 and 6400Hz is significantly less stable in dysphonic voices. As for running speech, the stability of spectral power is not such a good indicator of dysphonia, but there is a significant difference between normal and dysphonic voices in the power level of high-frequency bands (above 5300Hz). In addition, this means that sampling rates above 10.6ksps are needed for assessing running speech in spectral domain. Also, the results involving decorrelation times indicate that for short-time spectral analysis, frame rates above 100 frames/s should be preferred., (Copyright © 2013 The Voice Foundation. Published by Mosby, Inc. All rights reserved.)

Objective evaluation of the results of medical image segmentation is a known problem. Applied to the task of automatically detecting the glottal area from laryngeal images, this paper proposes a new objective measurement to evaluate the quality of a segmentation algorithm by comparing with the results given by a human expert. The new figure of merit is called Area Index, and its effectiveness is compared with one of the most used figures of merit found in the literature: the Pratt Index. Results over 110 laryngeal images presented high correlations between both indexes, demonstrating that the proposed measure is comparable to the Pratt Index and it is a good indicator of the segmentation quality.

The number of particles and their size distributions were measured in a rural area, during the summer, using a PCASP-X. The aim was to study the influence of wildfires on particle size distributions. The comparative studies carried out reveal an average increase of around ten times in the number of particles in the fine mode, especially in sizes between 0.10 and 0.14 μm, where the increase is of nearly 20 times. An analysis carried out at three different points in time--before, during, and after the passing of the smoke plume from the wildfires--shows that the mean geometric diameter of the fine mode in the measurements affected by the fire is smaller than the one obtained in the measurements carried out immediately before and after (0.14 μm) and presents average values of 0.11 μm.

Within this paper, the authors report on an experiment on automatic labelling of perceived voice roughness (R) and breathiness (B), according to the GRBAS scale. The main objective of the experiment has not been to correlate objective measures to perceived R and B, but to automatically evaluate R and B. For this purpose, a system has been trained that extracts the first mel-frequency cepstral coefficients (MFCC) of available sustained vowel phonations. Afterwards, a classifier has been trained to estimate the corresponding degrees of roughness and breathiness. The obtained results reveal a significant correlation between subjective and automatic labelling, hence indicating the feasibility of objective evaluation of voice quality by means of perceptually meaningful measures.

A new index is introduced in this article to measure the degree of normality in the speech. The proposed parameter has demonstrated to be correlated with the perceived hoarseness, giving an indication of the degree of normality. The calculation of such a parameter is based on a statistical model developed to represent normal and pathological voices. The modeling is built around Gaussian mixture models and Mel frequency cepstral coefficients. The proposed index has been named pathological likelihood index (PLI). PLI is compared with other aperiodicity features (such as jitter and shimmer), and measurements sensitive to additive noise (such as harmonics-to-noise ratio (HNR), cepstrum-based HNR, normalized noise energy, and glottal-to-noise excitation ratio). The proposed parameter is revealed to be a good estimator of the presence of pathology, showing lower correlation with noise, frequency, and amplitude perturbation parameters than these classical features among them., (Copyright © 2010 The Voice Foundation. Published by Mosby, Inc. All rights reserved.)

This study was carried out in the region of Castile and Leon, Spain, from 1980 to 1998 and analyzes the relationship between the number of monthly deaths caused by cardiovascular, respiratory and digestive diseases and three meteorological variables: temperature, pressure and humidity. One of the innovations in this study is the application of principal component analysis in a way that differs from its usual application: one single series representing the whole region was constructed for each meteorological variable from the series of eight weather stations. Annual and seasonal mortality trends were also studied. Cardiovascular diseases are the leading cause of death in Castile and Leon. The mortality related to cardiovascular, respiratory and digestive systems shows a statistically significant rising trend across the study period (an annual increase of 6, 16 and 4 per thousand, respectively). The pressure at which mortality is lowest is approximately the same for all causes of death (about 915 hPa), but temperature values vary greatly (16.8-19.7 degrees C for the mean, 10.9-18.1 degrees C for the minimum, and 24.1-27.2 degrees C for the maximum temperature). The most comfortable temperatures for patients with cardiovascular diseases (16.8 degrees C) are apparently lower than those for patients with respiratory diseases (18.1 degrees C), which are, in turn, lower than in the case of diseases of the digestive system (19.7 degrees C). Finally, the optimal humidity for patients with respiratory diseases is the lowest (24%) among the diseases, and the highest (51%) corresponds to diseases of the digestive system, while the optimal relative humidity for the cardiovascular system is 45%.

Mel-frequency cepstral coefficients (MFCC) have traditionally been used in speaker identification applications. Their use has been extended to speech quality assessment for clinical applications during the last few years. While the significance of such parameters for such an application may not seem clear at first thought, previous research has demonstrated their robustness and statistical significance and, at the same time, their close relationship with glottal noise measurements. This paper includes a review of this parameterization scheme and it analyzes its performance for voice analysis when patients are differentiated by sex. While it is of common use for establishing normative values for traditional voice descriptors (e.g. pitch, jitter, formants), differentiation by sex had not been tested yet for cepstral analysis of voice with clinical purposes. This paper shows that the automatic detection of laryngeal pathology on voice records based on MFCC can significantly improve its performance by means of this prior differentiation by sex., (Copyright 2009 S. Karger AG, Basel.)

This paper reports on the behaviour of Alnus glutinosa (alder) pollen grains in the atmosphere of Ponferrada (León, NW Spain) from 1995 to 2006. The study, which sought to determine the effects of various weather-related parameters on Alnus pollen counts, was performed using a volumetric method. The main pollination period for this taxon is January-February. Alder pollen is one of the eight major airborne pollen allergens found in the study area. An analysis was made of the correlation between pollen counts and major weather-related parameters over each period. In general, the strongest positive correlation was with temperature, particularly maximum temperature. During each period, peak pollen counts occurred when the maximum temperature fell within the range 9 degrees C-14 degrees C. Finally, multivariate analysis showed that the parameter exerting the greatest influence was temperature, a finding confirmed by Spearman correlation tests. Principal components analysis suggested that periods with high pollen counts were characterised by high maximum temperature, low rainfall and an absolute humidity of around 6 g m(-3). Use of this type of analysis in conjunction with other methods is essential for obtaining an accurate record of pollen-count variations over a given period.

The present work describes a new method for the automatic detection of the glottal space from laryngeal images obtained either with high speed or with conventional video cameras attached to a laryngoscope. The detection is based on the combination of several relevant techniques in the field of digital image processing. The image is segmented with a watershed transform followed by a region merging, while the final decision is taken using a simple linear predictor. This scheme has successfully segmented the glottal space in all the test images used. The method presented can be considered a generalist approach for the segmentation of the glottal space because, in contrast with other methods found in literature, this approach does not need either initialization or finding strict environmental conditions extracted from the images to be processed. Therefore, the main advantage is that the user does not have to outline the region of interest with a mouse click. In any case, some a priori knowledge about the glottal space is needed, but this a priori knowledge can be considered weak compared to the environmental conditions fixed in former works.

Plants of the Urticaceae family can develop into a pest on soils enriched with nitrogen. Urticaceae pollen is a biohazard because it elicits severe pollinosis. Pollen grains were sampled by using a Lanzoni seven-day-recording trap from February 1995-December 2000 in the atmosphere of the city of Ponferrada (Leon, North Western Spain). The Spearman test was used to analyse the statistical correlation between Urticaceae pollen and certain meteorological factors in different main pollination periods. Maximum values are reached in June and July, minimum levels are recorded in January and December. The parameters bearing the greatest positive influence on the occurrence of Urticaceae pollen grains are: temperature (maximum, minimum and mean), humidity (absolute, wet-bulb temperature, dew point and mixing ratio) and south western wind direction; negative parameters are: relative humidity, rainfall and period without wind. The highest correlation coefficients were obtained with temperature and wet-bulb. Absolute humidity and wet-bulb temperature yielded better correlation than relative humidity; hence, these two parameters must be included in this type of study. The use of one main pollination period or another in statistical analysis has an influence on the coefficient value. The behaviour of the pollen grains in the atmosphere during the year also influences the results.

The atmospheric particulates from the Caracus Valley in Venezuela and the fluvial particulates transported by the Tuy River into the Caribbean sea have been evaluated for Pb, Cu and Zn with the purpose of determining the contamination levels in the study area. The atmospheric particulate samples were collected in the city of Caracas using a low volume sampler whereas the fluvial particulate were collected at the mouth of the Tuy River. The particulate samples were analysed by flame or graphite furnace atomic absorption spectrometry depending upon the concentration levels of the heavy metal under study. The results obtained for the fluvial particulates enabled estimates to be made of the total anthropogenic flux of Cu (383 ton year(-1)), Pb (528 ton year(-1)) and Zn (865 ton year(-1)). These results yield annual per capita inputs for Cu (96 g),Pb (132 g) and Zn (216 g) which greatly exceed those from global anthropogenic emissions. The weighted average concentration of Pb (1.13 %) found in the atmospheric particulates was much higher than those for Cu (140 mg kg(-1)) and Zn (200 mg kg(-1)) and reflects the high motor car traffic in the Caracas Valley. The anthropogenic/natural ratios estimated in this study were as follows: 2.6 for Pb; 1.5 for Cu and 1.5 for Zn. This indicates that anthropogenic inputs for Cu, Pb, and Zn in the study area exceed those from natural sources, cars being the major source for Pb and industrial activities the major sources for Cu and Zn.

Ninety-one assessable patients with advanced oat cell and non-oat cell carcinoma of the lung were given AMSA on an intermittent every-3-week schedule. Starting doses ranged from 55 to 120 mg/m2, depending on the presence and severity of hepatic dysfunction. Three partial responses (two squamous cell carcinomas, one adenocarcinoma) of short duration were documented. The major toxic effect was leukopenia (44%). AMSA does not appear to have sufficient antitumor activity to warrant further investigation in advanced lung cancer.