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An in vitro assay to quantify satellite cell activation using isolated mouse myofibers.

Authors :
Canibano-Fraile R
Boertjes E
Bozhilova S
Pijnappel WWMP
Schaaf GJ
Source :
STAR protocols [STAR Protoc] 2021 Apr 23; Vol. 2 (2), pp. 100482. Date of Electronic Publication: 2021 Apr 23 (Print Publication: 2021).
Publication Year :
2021

Abstract

Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.<br />Competing Interests: The authors declare no competing interests.<br /> (© 2021 The Author(s).)

Details

Language :
English
ISSN :
2666-1667
Volume :
2
Issue :
2
Database :
MEDLINE
Journal :
STAR protocols
Publication Type :
Academic Journal
Accession number :
33997810
Full Text :
https://doi.org/10.1016/j.xpro.2021.100482