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An in vitro assay to quantify satellite cell activation using isolated mouse myofibers.
- Source :
-
STAR protocols [STAR Protoc] 2021 Apr 23; Vol. 2 (2), pp. 100482. Date of Electronic Publication: 2021 Apr 23 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.<br />Competing Interests: The authors declare no competing interests.<br /> (© 2021 The Author(s).)
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 2
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 33997810
- Full Text :
- https://doi.org/10.1016/j.xpro.2021.100482