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We modeling myocardial infarction female Wistar rats (N=126). We used morphomethry for examination of infarction zone from 2 h to 30 days after operation. The study comprised three groups of animals: "Control", "FGF" with 100 Hz FCGF2 injection into left ventricle, "anti-FGF" with 2 microg monoclonal antiFCF2 antibodies injections at period 1.5 h, 6 h and 3 day after operation. We founded that FCF2 injections leaded to enlarged of infiltrative inflammatory stage and take effect on the early collagen synthesis in infarction regions (from 3 days in group with FGF2 injection vs. 7 days in control group). Opportunity,injection of anti-FCF2 antibodies leaded to decreasing collagen volume on 30 day after infarction (19.15 +/- 1.22% in "anti-FGF" group vs. 41.71 +/- 1.30% in "Control" group, p < 0.05).

The activities of lectins and peroxidase and lignin content were studied in the perilesional area of leaves in two tobacco species (Nicotiana tabacum L., cultivar Samsun NN, and N. glutinosa L.) inoculated with tobacco mosaic virus. The development of hypersensitivity response proved to be accompanied by a complex spatial and temporal distribution of lectin activity. The area 5-9 mm away from the lesion center was characterized by the highest activity of loosely bound membrane lectins eluted with 0.05% Triton X-100. In the fraction of tightly bound membrane lectins (eluted with 0.5% detergent), lectin activity decreased during the first two days but increased on day 4 after inoculation. The activity of loosely bound membrane lectins increased in the leaf areas distant from the lesion. Two-phase dynamics in the interlesional area were also observed for lectin activity in the tightly bound membrane fraction (decrease on day 2 days and increase on day 4 after inoculation) and for peroxidase activity (increase on day 2 days and decrease on day 4). The relationship between the dynamics and spatial distribution of lectins in the perilesional area and the possible involvement of these proteins in pathogen-induced changes in photosynthesis are discussed.

Affective phases developing in personality disorder (index-sample--98 patients) were compared to those in cyclothymia (85 patients--control group). A preference of phase dynamics in the group of abnormalities relating to ICD-10 item "Borderline personality disorder" was confirmed. In line with a concept considering personality disorders as clinical syndromes, patients of the index-group have personality disorders with the signs of psychopathological diathesis determined by vulnerability to affective disorders. Affective phases are interpreted not only as an expression of a specific type of personality disorders dynamics but as an emergence of affective pathology, which is alternative to endogenous one both by modus of constitutional predisposition and clinical parameters (egosyntonic moderating of the phase, domination of negative affectivity in its structure, amphitymic duality of pathologically altered affect).

Using clinical and psychopathological method, 134 patients who met the ICD-10 criteria of diagnosis of schizoaffective psychosis (F25), were examined. Relatively small prognostic informative value of standard schizoaffective psychosis differentiation into affective- and schizo-dominant types was found. Subtyping of schizoaffective psychosis according to peculiarities of delusion formation in the picture of the disease attacks proved to be more significant. Given clinico-psychopathological features of circular affective and delusional disorders and their pathokinesis, as well as dynamics types and disease outcome, a schizoaffective psychosis systematization has been elaborated. That includes 3 nosologic types: "nuclear" type with non-progressive phase dynamics; "marginal" (intermediate) type and schizoaffective type of attack-like progressive schizophrenia. The concept of nosologic independence of schizoaffective psychosis ("nuclear" type) is discussed.

A total of 168 patients were examined presenting with different clinical and roentgenological forms of acute pneumonia, such as focal one with an acute course, focal protracted, croupous with an acute course, croupous protracted, abscessing ones. Blood serum elastinase-like activity was studied. The complicated forms of pneumonia were found to be accompanied by two-phase dynamics of elastinase-like activity, such that it soars by day 10-15 of the disease process and declines by day 20-15, unlike the prognostically favourable forms of the condition where the initial reduction of this parameter turns to its gradual normalization.

It has been shown that paradoxical sleep (PS) total time (TT) in euthermia phase of hibernation cycle (winter sleep) can be compared with its mean values in euthermia season. Ultradian fluctuations of PS episodes duration, TT and phase number as well as bradycardia microintervals are revealed in euthermia phase dynamics. Amplitude of these fluctuations increases before hibernation entry; in transitional "start" period PSTT increases up to maximum critical values. In hibernation cycle PSTT rapidly decreases at the first stage owing to reduction of PS phase number and their duration. These data support and supplement earlier suggested hypothesis about endogenous "swinging up" of ultradian rhythms of sleep and thermoregulation promoting stage-to-stage expansion of homeostasis temperature limits in various critical periods of preparation and entry of homeotherms into natural hypometabolic states (torpor, hibernation and others).

Age peculiarities of absorption of 14C-biotin by the blood cells and plasma proteins in vitro are studied. The phase dynamics of 14C-biotin absorption by the blood cells and binding by plasma proteins is found. The reliable ontogenetic differences in absorption of 14C-biotin by the blood cells and plasma proteins are determined. The biotin-binding in vitro by the blood cells and plasma proteins decreases and becomes slower in old rats as compared with young ones.

A phase dynamics in body temperature and thermoproduction was shown in the course of cooling of mice. 3 periods of various intensity were noted in thermocompensatory reactions. Within the first period content of noradrenaline was maintained in the vitally important tissues (brain, heart and brown adipose tissues); later on the content of noradrenaline was distinctly decreased. The blockade of ganglions and beta-adrenoreceptors inhibited markedly the thermoproduction and resistance to cooling. The main thermocompensatory reactions were realized via beta 1-adrenoreceptors; blocking of beta 1-adrenoreceptors by means of practolol was more effective as compared with the influence of N-isopropylmethoxamine--inhibitor of beta 2-adrenoreceptors. Importance of beta 1-adrenoreceptors in thermoregulation is discussed.

Mice with transplantable Harding-Passey melanoma were treated with polysaccharide mannan (50 mg/kg), cyclophosphamide (20 and 100 mg/kg) and their combinations. Mannan proved to possess considerable antitumor properties. It potentiated the therapeutic effect of cyclophosphamide and diminished its toxicity. Mannan treatment resulted in two-phase dynamics of cyclic nucleotide system in mouse liver and melanoma.

Leucocytes adsorb by two orders of magnitude more labeled nicotinic acid ([14C]Na) than erythrocytes (as calculated on a per cell basis). The dynamics of binding of labeled vitamin by leucocytes is biphasic with the formation of predominantly [14C]nicotinic coenzymes already at very short time intervals after their injection to rats. Simultaneous injections of thiamine, riboflavin, lipoate and pantotenate increased the level of total labeled nicotinate metabolites in the blood and leucocytes 2.1- and 4.1-fold, respectively. The metabolism of subcutaneously injected [14C]NA was predominantly localized in the digestive system with a markedly pronounced two-phase dynamics of changes of the level of total labeled metabolites in the liver and small intestine concomitant with their secretion together with digestive juices. The functionally coupled vitamins injected simultaneously sharply increased the incorporation of the total label into liver tissues (up to 45% of the injected dose against 33% in the control) and the increase in the level of [14C]pyridine nucleotides. Similar effects were observed upon accumulation of labeled metabolites of [14C]NA in small intestine membranes. The increase in the maximal accumulation of nicotinate under effects of other group B vitamins in brain, heart and spleen tissues correlated with the dynamics, of their accumulation in the blood. In the postmaximal period in cardiac muscle and brain tissues, the second increase in the [14C]NA binding correlated with the dynamics of its accumulation in the digestive system.(ABSTRACT TRUNCATED AT 250 WORDS)

Pantothenate, penetrating the liver tissue after intraperitoneal administration into young rats at a dose 100 mumole/kg was metabolized to unidentified substances within 15-30 min without accumulation of 4-phosphopantothenate. Within subsequent 2-4 hrs period pantothenate, 4-phosphopantothenate, CoA and considerably higher amounts of the unidentified metabolites (apparently symmetric and mixed (disulphides) were simultaneously accumulated in liver tissue; the latter substances determined the subsequent prolonged and essential elevation in content of CoA in the liver tissue. The phase dynamics of accumulation in liver tissue of the administered pantothenate is due to its recirculation from intestine and to alteration in the activity of pantothenate metabolizing enzymes, particularly those, which catalyze the reactions of thiodisulphide metabolism.

Metabolism of serotonin was studied in duodenum of rabbits in acute kidney ischemia (from 15 min to 1 hr). Estimations of serotonin and 5-hydroxy indolacetic acid content and the activities of 5-hydroxytryptophane decarboxylase (5-HTD) and monoamine oxidase (MAO) using serotonin as a substrate were carried out. A complicated phase dynamics in the intestine of main patterns of serotonin metabolism was observed in acute kidney ischemia. Within the ischemic period content of serotonin and the MAO activity correlated with the activity of 5-HTD in the duodenal tissue. The alterations in the activity of MAO from intestine suggest that this enzyme was especially important in the serotonin metabolic conversions studied.