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Electro-Fenton process could remove pollutant in in tannery industry wastewater. The objectives of this is used to know the effect of electro-Fenton on, ammonia (NH3), chromium (III) (Cr(III), total suspended solid (TSS), biological oxygen deman d (BOD), and chemical oxygen demand (COD) removal in tannery wastewater treatment. The voltage variation used to trigger the electro-Fenton reaction was 6V, 8V, and 10V. Observation time at 0, 30, 60, 90, and 120 mins was used to see changes in the sample. The results obtained are electro-Fenton method has an effective time of 30 mins to removal efficiency of 60.7%, 32.9%, 72.8%, 53.4%, and 53.4% for NH3, Cr(III), TSS, BOD, and COD respectively. Pollution from tannery effluent can be eliminated to a sufficient extent using the electro-Fenton technique.

Sengketa tanah akhir-akhir ini menjadi isu yang sangat sentral yang menjadikan skala prioritas segera bisa terselesaikan, ketidakberdayaan lembaga peradilan untuk menyelesaikan penyelesaian sengketa tanah diberbagai wilayah, menjadikan kepercayaan masyarakat berkurang. Oleh karena itu diperlukan penyelesaian lain (opsi lain), untuk mengatasi berbagai kasus penyelesaian pertanahan di negerini ini. Yaitu penyelesaiannya di luar pengadilan (Non Litigasi) Kota Depok, daerah Propinsi Jawa Barat, berbagai persoalan pertanahan banyak sekali yang tidak bisa diselesaikan, ketidakmampuan lembaga pemerintah Kantor Pertanahan Kota Depok, untuk segera mengatasi mungkin persoalan tersebut biar tidak menambah persoalan menjadi semakin sulit. Permasalahan bidang pertanahan di wilayah Kota Depok Jawa Barat mempengaruhi berbagai faktor, karena wilayah depok yang bersinggungan langsung dengan pusar pemerintahan DKI Jakarta, sehingga kebutuhan tanah menjadi hal yang tidak bisa dihindari, hal ini menjadi permasalahn dibidang pertanahan menjadi eskalali tinggi. Untuk mencari solusi agar permasalahan tersebut dapat diatasi, harapan permasalahan selesai maka tanah dapat dimanfaatkan secara ekonomi. Salah satu alternatif solusi untuk menyelesaikan penyelesaian pertanahan dengan melalui Mediasi (Non Litigasi), disisi lain hal tersebut dapat dilakukan secara efektif dan efisien, dengan biaya murah, waktu singkat, dengan sebuah syarat bahwa para pihak dalam penyelesaian pertanahan dapat menerima dengan rasa keadilan. Melalui kegiatan pengabdian kepada masyarakat ini dalam bentuk penyuluhan hukum yang bertemakan “Penyuluhan Hukum Penyelesaian Sengketa Tanah Atau Lahan Melalui Jalur Non Litigasi atau Diluar Ranah Pengadilan Diwilayah Kelurahan Pangkalan Jati Baru Kecamatan Cinere Kota Depok” diharapkan bisa memberikan solusi atau opsi bagi permasalahan yang saat ini masih terjadi di Kelurahan Pangkalan Jati Baru Kecamatan Cinere Kota Depok. Kegiatan pengabdian kepada masyarakat ini sudah sebelumnya pernah dilaksanakan dua kali tahun 2021 dan tahun ini 2022 dengan tema berbeda. Kegiatan Abdimas ini dilakukan dengan menggunakan metode ceramah, diskusi dengan menghadirkan perangkat kelurahan, tokoh masyarakat, ketua RT, RW yang ada dilingkungan kelurahan Pangkalan Jati Baru. Kesimpulan Dari hasil kegiatan Abdimas masyarakat sangat antusias banyaknya pertanyaan-pertanyaan yang dilontarkan kepada penyaji dan keingintahuan tentang pertanahan sangat serius, peserta Abdimas menyarankan kegiatan pengabdian masyarakat ini bisa berkesinambungan memberikan wacana atau gambaran hal hal yang berkaitan dengan penebangan lahan atau dengan tema yang lain

This article starts from the premise that the ordinary is the mystical. It does so by delving into the dynamic relationship between Spirituality and Public Theology against the backdrop of Christianity’s societal roles. It explores how Christian theology extends beyond private faith to address broad societal issues. Through a critical examination of Public Theology’s distinct contributions to contemporary discussions, the article emphasises the necessity of engaging Spirituality – with its focus on the divine-human relationship into this discourse. Contribution: This synthesis aims to enrich our comprehension of Christianity’s impact on public life, advocating for a faith that actively contributes to societal transformation across diverse contexts.

Abstract Bioengineered probiotics enable new opportunities to improve colorectal cancer (CRC) screening, prevention and treatment. Here, first, we demonstrate selective colonization of colorectal adenomas after oral delivery of probiotic E. coli Nissle 1917 (EcN) to a genetically-engineered murine model of CRC predisposition and orthotopic models of CRC. We next undertake an interventional, double-blind, dual-centre, prospective clinical trial, in which CRC patients take either placebo or EcN for two weeks prior to resection of neoplastic and adjacent normal colorectal tissue (ACTRN12619000210178). We detect enrichment of EcN in tumor samples over normal tissue from probiotic-treated patients (primary outcome of the trial). Next, we develop early CRC intervention strategies. To detect lesions, we engineer EcN to produce a small molecule, salicylate. Oral delivery of this strain results in increased levels of salicylate in the urine of adenoma-bearing mice, in comparison to healthy controls. To assess therapeutic potential, we engineer EcN to locally release a cytokine, GM-CSF, and blocking nanobodies against PD-L1 and CTLA-4 at the neoplastic site, and demonstrate that oral delivery of this strain reduces adenoma burden by ~50%. Together, these results support the use of EcN as an orally-deliverable platform to detect disease and treat CRC through the production of screening and therapeutic molecules.

In previous studies, the use of mineral wools as an onsite-supporting media in water bodies showed satisfactory results in removing organic pollutants. However, the analytical method chosen is still very conventional. This study aims to model the removal of organic pollutants represented by the COD value to determine the mineral performance. The data used in this study are field scale data using two types of mineral wools, namely type I (density 80 kg/m3 and water retention 95%) and type II (density 120 kg/m3 and water retention 92%). There are form variations of mineral wool namely cubes and blocks on each type, which are placed respectively in segments 2, 4, 8, and 9, on the Cikapayang River. The modeling results show that the most optimal type of mineral wool to remove COD is type II mineral wool-cube form at segment 4 using the first order kinetics equation. The reaction coefficient (k) obtained is 0,5378/s, standard deviation value is 14,532 mg/L COD, and the coefficient of determination is 0,1025. The kinetics value of the reaction removal and equations obtained are used to make modeling in Matlab R2020b application to determine the dimensions of the mineral wool required to remove pollutants.

Urban water management efforts are essential in encouraging the fulfillment of the SDGs targets. One action that can be done is to approach the calculation of the actual water consumption value. This research conducted a survey of domestic water consumption in Bandar Lampung City to obtain comprehensive information. Bandar Lampung City was chosen because it is one of the cities on the island of Sumatra with a high economic growth rate. Lampung Province is the top 3 province in Sumatera Island with the higher Gross Domestic Product Growth on 2022, with Bandar Lampung as its capital city. Furthermore, water consumption patterns were analyzed for various activities, socio demographic conditions, and the community's economy. The results showed that of the 404 samples, the average water usage was 195.08 liters/person/day, with the dominant activities in use including bathing (66.84 liters/person/day), flushing the toilet (35.71 liters/person) / day), and ablution (29.74 liters/person/day). Furthermore, the variable number of family members in one house and income level have significant different on total domestic water consumption. The results obtained in this study are expected to assist in making decisions regarding urban water management plans.

Total Suspended Particulates (TSP) in ambient air contain metallic elements and can be inhaled by humans. Inhaled metal elements risk public health with long-term exposure because metallic elements are carcinogenic in the human body. This study aimed to analyze metallic elements and assess their risk to public health in the Bandung area and its surroundings. The metal elements analyzed in this research are Pb, Al, and Mn. Ambient air sampling was carried out in five industrial areas: Bandung Wetan, Buahbatu, Cibeureum, Padalarang, and Cimahi. High Volume Air Sampler (HVAS) is for sampling TSP in ambient air, and X-Ray Fluorescence (XRF) was used to analyze metallic elements. The results of the study showed that the Cimahi location gave the highest TSP level (126.7 g/m3) and the lowest is Padalarang (15.1µg/m3). The metal elements concentrations, Pb, Al, and Mn were varied widely of 7.1–29.21 ng/m3, 1054.9–1700.1 ng/m3, and 8.91–14.79 ng/m3, respectively. Risk analysis was determined by calculating ADDinhale, Hazard Quotient (HQ), and Hazard Index (HI) to determine whether there is a potential non–carcinogenic effect on public health. Each industrial area gives an HI value > 1, and Buahbatu has the highest HI. This study proves that Pb significantly contributes to the increased risk of community exposure to non-carcinogenic effects.

Background and Aim: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain in veterinary and human medicine is a growing global problem. This study aimed to evaluate MRSA isolates of human and animal origin against various antibiotics in Yogyakarta, Indonesia. Materials and Methods: The susceptibility test was carried out by the disk diffusion method using Mueller-Hinton agar against nine antibiotic disks. Methicillin-resistant S. aureus strains were genetically confirmed through mecA gene detection encoding for methicillin resistance by polymerase chain reaction. Results: All 240 S. aureus strains isolated from animals and humans were resistant to penicillin G (P) (100% and 99%, respectively), followed by ampicillin (AMP), amoxicillin (AML), oxacillin (OX), erythromycin (E), clindamycin (DA), tetracycline (TE), gentamicin (GEN), and ciprofloxacin (CIP). Eighty-three MRSA strains were resistant to OX (100%), P (100%), AMP (99.27%), AML (95.52%), E (87.77%), TE (71.33%), DA (63.24%), GEN (38.81%), and CIP (26.87%). Conclusion: The antimicrobial resistance pattern of S. aureus human isolates was similar to their animal counterpart, with 77.20% of MRSA strains classified as multidrug-resistant (MDR) bacteria. These findings indicate an increase in MDR S. aureus strains of animal origin in Yogyakarta, thus raising public health concerns about MRSA zoonotic spread.

Nesta pesquisa, faz-se a indagação sobre quais são os perfis gerenciais percebidos como necessários para a execução das funções do Tribunal Regional Eleitoral do Acre. Objetiva-se compreender os perfis gerenciais predominantes nos colaboradores da instituição. Tendo isso, com o pouco conhecimento por parte das organizações públicas sobre as competências gerenciais necessárias para o desenvolvimento das atividades no TRE-AC, deu-se o problema da pesquisa onde se buscou saber os perfis gerenciais predominantes dos servidores do TRE-AC. A abordagem é quantitativa e um estudo quantitativo com análise estatística. O resultado alcançado demonstrou a predominância dos papéis dos colaboradores como Inovador e Negociador. Sendo assim, a organização pública deve utilizar o mapeamento das competências gerenciais dos colaboradores para o desenvolvimento do treinamento na função.

The higher-order thinking ability of students in Indonesia is still in the low category. This research aims to see the effect of the application of a problem-based learning model on higher-order mathematical thinking skills in terms of student's initial abilities and mathematical beliefs. This research uses a quasi-experimental design with a Non-Equivalent Control Group Design. Data on students' higher-order mathematical thinking skills were taken using a test instrument. This research was conducted at SMP Negeri 27 Jakarta with a total of 165 students obtained from cluster random sampling. Based on the analysis of the three-way ANOVA test, it was found that the problem-based learning model had a positive effect on students' higher-order thinking skills. Problem-based learning model gives better results than conventional learning models. This also applies to students with the initial ability and mathematical beliefs in the high category. Problem-based learning can be an alternative for teachers to improve students' higher-order mathematical thinking skills.

Crude oil contamination is considered highly toxic and poses a significant environmental problem. As an alternative, the laccase enzyme showed high performance for removing various toxic contaminants, particularly oil-contaminated soil (i.e., total petroleum hydrocarbon). However, previous studies mainly tested the performance of laccase under irrelevant environmental conditions (i.e., artificially contaminated soil) with a limited number of soil samples, which can lead to bias optimization results for assessing laccase performance. Two types of natural oil-contaminated soils were tested under various reaction times and various concentrations of laccase extracted from Marasmiellus palmovorus. In addition, response surface methodology was used to find the maximum degradation of total petroleum hydrocarbon (TPH). The maximum degradation of TPH from soil A and soil B were 48.57% and 54.1%, respectively. Moreover, the performance of the laccase enzyme for oil recovery was also tested, with the percentage of oil recovery being 9.89% and 10.1 % for soil A and soil B, respectively. SARA fraction analysis indicated that laccase enzyme preferentially degraded highly polar SARA fraction (i.e., asphaltene and resin). In general, the application of laccase for the enzymatic remediation of oil-contaminated soils was practical. Hence, the use of laccase for environmental application is still promising.

O presente artigo de pesquisa trata a respeito da abordagem no corpo docente dos cursos superiores do Instituto Federal do Acre – IFAC do campus Rio Branco. Nesse estudo, faz-se o questionamento sobre quais são as competências gerenciais percebidas nos professores para a execução das funções docentes, durante a pandemia de Covid-19, além do mapeamento dessas capacidades constatadas. Então, utilizou-se o modelo de Quinn et al. como base de análise, esse modelo se define em vinte e quatro competências gerenciais, conceituando-as e classificando-as em oito papéis gerenciais. O objetivo é analisar as competências gerenciais dos professores de graduação de diversos cursos no IFAC campus Rio Branco, em conformidade com o modelo de Quinn. A abordagem é quantitativa com a coleta de dados realizada por meio de questionário, observando os resultados pelos gráficos de pizza e radar. A escolha do tema revela-se por não haver uma pesquisa nesse ramo no campus Rio Branco, logo nota-se a necessidade de aplicar aos professores. Buscou-se potencializar os campos de conhecimentos da Administração, Educação e Gestão. O resultado alcançado despertou nos docentes habilidades de inovador, produtor, diretor, monitor, facilitador, mentor, com maior ênfase em coordenador, no modelo de processos internos e desequilíbrio no perfil negociador, no modelo de sistemas abertos. Nesse sentido, configurou-se relevante qualificação do corpo docente no período de pandemia.

Abstract Splenunculus, or congenital accessory spleen, is a benign anatomical variation, and is rarely of clinical consideration in routine clinical practice. We describe a patient who presented with synchronous herpes simplex 1 viraemia and myelosuppression, with a splenunculus mimicking splenomegaly, and we discuss the implications on clinical practice, investigations, and management.

Pendahuluan: Tension Type Headache (TTH) dapat dipengaruhi banyak faktor, beberapa di antaranya adalah stres dan kadar hormon kortisol. Beberapa penelitian telah menganalisa hubungan nyeri kepala terhadap kortisol, dan stres. Namun, jarang ada penelitian yang menghubungkan ketiganya. Tujuan: Membuktikan adanya hubungan antara intensitas nyeri kepala, stres psikologis dan kadar kortisol serum pada penderita TTH. Metode: Jenis penelitian adalah analitik observasional dengan rancangan cross sectional dengan uji korelasi. Hasil: Pada penelitian ini didapatkan 42 subyek penelitian. Dari pengolahan data, didapatkan p= 0,594. Yang berarti tidak ada korelasi signifikan antara intensitas nyeri kepala dengan stres psikologis. Sementara itu, pada intentistas nyeri kepala dengan kadar kortisol didapatkan korelasi signifikan dengan p= 0,04. Pada stres dan kadar kortisol tidak ditemukan korelasi signifikan dengan p= 0,154. Kesimpulan: Ada hubungan yang signifikan antara intensitas nyeri kepala dengan kadar kortisol serum. Sebaliknya, pada intensitas nyeri kepala dengan stres psikologis dan stres psikologis dengan kadar kortisol serum, tidak memiliki hubungan yang signifikan.

Interest in Public Theology is growing across the African continent. However, there are some important critiques of Public Theology and public theologians in Africa and from Africa. This article outlines three important critiques of Public Theology within the variety of African contexts. Having done so, it seeks to engage in a process of critical reflection on the two constituent concepts related to an ‘African Public Theology’. First, it considers what we might mean when we speak of ‘Africa’ or ‘African?’ It does so by engaging relevant literature on the subjects of decolonisation and Africanisation as they relate to knowledge production and African theological reflection. Second, we ask, what do we mean when we speak of ‘Public Theology?’ Two broad approaches are presented and critically considered. First, we discuss a descriptive approach to Public Theology. Then we consider a more prescriptive understanding of Public Theology. In each instance we relate to both the critiques of Public Theologies in African contexts, and to the principles of decolonisation and Africanisation, as presented in relevant literature related to the debates and the context. The intention of this article is to take the three identified critiques of ‘African Public Theology’ seriously, and by means of critical conceptual engagement, to keep an important conversation on Public Theology in and from Africa alive. Contribution: This article contributes towards contemporary debates on the relationship between faith and public life in South Africa. South Africa remains a deeply religious society. Religion plays an important role in the formation of moral values, social norms, and dominant practices in public life. Gaining a clearer understanding of what Public Theology is and how it is practiced, helps to further our critical academic understanding of the concept and its practice in contemporary life.

How do we know who we are? What sources can we draw upon in order to explain and understand the complex notions of identity and consciousness? This article revisits this debate and argues that African approaches the consciousness and identity cohere with Wentzel van Huyssteen’s post-foundational theology. Post-foundational theology offers a transverse rationality that operates between explanatory power and truth. The impetus for the research that informs this article emerged from a conversation with Prof. Van Huyssteen in 2000. The conversation set the author on a path of exploration which led to the discovery of the richness of African religious, philosophical and social resources on identity and consciousness. The outcome was an integrated approach to identity known as a ‘generous ontology’ that draws upon subjective, objective, inter-subjective and inter-objective sources of knowledge. The article concludes that an African approach to consciousness, as a post-foundational theological contribution, helps us to offer clear explanations and deeper truths in relation to our understanding of identity and consciousness. Intradisciplinary and or interdisciplinary implications: This article presents a post-foundationalist argument for the inclusion of African theological notions of identity and consciousness in the debates of this field that take place at the intersections of faith and science. The outcome textures our explanations and deepens our understandings of transdisciplinary approaches to identity and consciousness.

An Ocular Sebaceous Carcinoma (OSC) is a rare malignant tumor for which initial clinical and pathological diagnosis is often incorrect. OSCs can mimic Squamous Cell Carcinomas of the Conjunctiva (SCCC). The aim of this study was to find microRNA biomarkers to distinguish OSCs and SCCCs from normal tissue and from each other. Clinical OSC and SCCC case files and the corresponding histopathological slides were collected and reviewed. Micro dissected formalin-fixed paraffin-embedded tumor and control tissues were subjected to semi-high throughput microRNA profiling. MicroRNA expression distinguishes OSCs and SCCCs from corresponding control tissues. Selected differentially expressed miRNAs were validated using single RT-PCR assays. No prognostic miRNAs could be identified that reliably predict SCCC metastasis or OSC recurrence. A comparison between OSCs (n = 14) and SCCCs (n = 18) revealed 38 differentially expressed microRNAs (p < 0.05). Differentially expressed miRNAs were selected for validation in the discovery cohort and an independent validation cohort (OSCs, n = 11; SCCCs, n = 12). At least two miRNAs, miR-196b-5p (p ≤ 0.05) and miR-107 (p ≤ 0.001), displayed a statistically significant differential expression between OSCs and SCCCs with miR-196b-5p upregulated in SCCCs and miR-107 upregulated in OSCs. In the validation cohort, microRNA miR-493-3p also showed significant upregulation in SCCCs when compared to OSCs (p ≤ 0.05). ROC analyses indicated that the combined miR-196b-5p and miR-107 expression levels predicted OSCs with 90.0% sensitivity and 83.3% specificity. In conclusion, the combined testing of miR-196b-5p and miR-107, can be of additional use in routine diagnostics to discriminate OSCs from SCCCs.

This article argues for a rediscovery of a theology of work in South Africa that is based on the Protestant notion of calling and vocation. Such a view has the primary intention of emphasising obedience and faithfulness to God rather than self-fulfilment or achievement as the intentions of work. Such an approach can empower and equip the church and individual Christians for effective and faithful living in all spheres of life – both private and public. The article shows that the influences of theological dualism, an unbalanced view of the clergy as primary agents of ministry and mission, and a structures-centred view of ministry and mission detracted from the importance of the church’s ministry in numerous spheres of society. A consequence of this was the introduction of a subtle dualism between faith and work. In response to this, the article considers how the church could become an agent of mission and transformation in the world of work. The conclusion of this article is that the South African church could benefit from revisiting and rediscovering a theology of work that is based on the Protestant emphasis of calling and vocation in the public sphere. Contribution: While this article engages the traditional protestant theological notions of calling and vocation, it argues that reconsidering these notions in relation to the contemporary world of work can renew a theology of work and ministry for South African churches to serve their members in achieving God’s will in society.

Background: There has been speculation that IOP-lowering medication, which increases aqueous humor outflow, increases the risk of metastatic uveal melanoma (UM). This hypothesis has not been studied previously but is relevant for UM patients who use IOP-lowering medication. The aim of the current study is to assess the association between the use of intraocular pressure (IOP)-lowering medication and the risk of metastatic UM, and mortality. Methods: A retrospective cohort study, in which patients from the Rotterdam Ocular Melanoma Study were included from 1986 onwards. Medical records were evaluated for use of IOP-lowering medication at baseline (i.e., before diagnosis). For each IOP-lowering medication, we divided patients into two groups for comparison (e.g., patients with alpha2-agonist use and patients without alpha2-agonist use). All patients underwent regular ophthalmic examinations and routine screening for metastasis. Survival analyses were initiated to compare groups in each IOP-lowering medication group. In addition, secondary analyses were performed to examine the association between IOP and the development of metastatic UM, and mortality. Results: A total of 707 patients were included of whom 13 patients used prostaglandin or pilocarpine at baseline. For alpha2-agonist, beta-blocker, carbonic anhydrase inhibitor, and oral IOP-lowering medication these were 4, 14, 11, and 12 patients, respectively. The risk of metastatic UM (choroid and ciliary body melanoma) among the prostaglandin/pilocarpine users was significantly higher than controls (HR [95% CI]: 4.840 [1.452–16.133]). Mortality did not differ significantly among the IOP-lowering medications groups, except for the prostaglandin or pilocarpine group (HR [95% CI]: 7.528 [1.836–30.867]). If we combined all IOP-lowering medication that increase aqueous humor outflow, the risk (HR [95% CI]) of metastatic UM and mortality was 6.344 (1.615–24.918) and 9.743 (2.475–38.353), respectively. There was an association between IOP and mortality, but not for the onset of metastatic UM. Conclusion: The use of topical prostaglandin or pilocarpine may increase the risk of metastatic UM and mortality compared to patients without prostaglandin or pilocarpine use. Therefore, use of IOP-lowering medication which increases aqueous humor outflow, should be avoided in patients with (presumed) UM.

Data mining tidak hanya digunakan untuk membahas hal-hal yang berbau informatika, akan tetapi bisa digunakan untuk meneliti berbagai hal, bahkan pola untuk menemukan gejala diabetes pada pasien . Pada paper ini membahas cara memprediksi apakah seseorang mengidap penyakit diabetes menggunakan Data Set yang telah diperoleh dari Machine Learning terlebih dahulu. Pelatihan dilakukan dengan metode Bayesian Regularization Neural Network (RBNN) yang diharapkan dapat memberikan hasil yang diharapkan sesuai dengan prediksi pada penelitian kali ini.

Nanocomposites of UiO-66 and graphene oxide (UiO-66_GO) were prepared with different GO contents by a one-step hydrothermal method, and their photocatalytic activities for the degradation of carbamazepine (CBZ) were investigated under ranges of GO loading, catalyst dose, initial pollutant concentration, and solution pH. The UiO-66_GO nanocomposites showed photocatalytic rate constant up to 0.0136 min−1 for CBZ degradation and its high overall removal efficiency (>90%) in 2 h. The photocatalytic rate constant over the UiO-66_GO nanocomposite was about 2.8 and 1.7 times higher than those over pristine GO and UiO-66, respectively. The enhancement of photocatalytic activity by GO was attributed to increased surface area and porosity, improved light absorption, and narrowed band gap. The composite also showed substantial recyclability and stability over five consecutive cycles of photocatalytic degradation. The experimental results indicated that O2●− and OH● are the responsible radicals for photocatalytic degradation, which helped us propose a photocatalytic mechanism for the enhanced CBZ photodegradation. This work provides a reference for the development of GO-based composite photocatalysts and expands the application of UiO-66 as a photocatalyst for the degradation of persistent micropollutants in water.

Some 20 years after the dawn of participative democracy, there is little noticeable or substantial change in the living conditions of the average South African. The country remains divided by race, class and economics. Poverty, inequality and racial enmity remain looming challenges to human flourishing and social transformation. Some have begun to ask whether forgiveness for the sins of colonialism and apartheid are possible. This article engages with the (im)possibilityof forgiveness as it is presented in Matthew 18:15-35. In particular, it does so from the bilingual perspective of a public theological engagement with the text and its contemporary readers in South Africa. By reading the text from an integral All Quadrants All Levels (AQAL) approach this article extrapolates a textured understanding of forgiveness that ‘possibilises’ the (im)possiblity of forgiveness between racially and socially divided groups of readers.

This article engages with the Missional Framework Document of the Dutch Reformed Church (DRC) from the perspectives of solidarity with the poor and justice for South African society. The democratic South Africa continues to face significant socio-economic problems and an increasingly dissatisfied population. In the light of this, the article presents an introductory conversation with the Missional Framework Document in order to ask whether it offers an adequate response to South Africa�s current contextual challenges. The lens through which this article will engage the Framework Document is the theological paradigm of justice, specifically the theory of justice presented by Nicholas Wolterstorff as well as some important contributions from contemporary South African scholars who advocate for a theology from the margins of society. It is contended that the DRC remains a predominantly white middle-class church. This social, economic and political location has an impact on the missional theology of the church as expressed in the Framework Document. Hence, we engage with the Missional Theology of the DRC by means of a paradigm that operates from the �underside� or the economic, political and social �margins� of South African society. We argue that any missional theology aimed at furthering God�s Kingdom in South Africa at present must develop in community with the marginalised majority of the nation. The critical engagement with the Framework Document will be done by sketching a theological landscape where current contextual realities are brought into relief against a kairos moment on which the efficacy of this church�s mission, indeed its public work and witness, is being called into question.Intradisciplinary and/or interdisciplinary implications: This interdisciplinary study in Systematic Theology and Ethics engages the missional theology found in the Missional Framework Document of the Dutch Reformed Church. Methodologically, the research advocates for a contextual engagement with the missional theology proposed by this important document. The outcome of this contextual theological consideration is an invitation for the Church to consider the ethics of justice as an important aspect of their approach to faithful Christian mission in the South African social, economic and political context.

This article takes the form of an appreciative contextual response to the notion of ‘just health’ that is formulated in Jean-Pierre Wils’ article, ‘Is there a future for medical ethics?’ It approaches the notion of just health in the South African context from a public theological vantage point. The article addresses the issues of justice, care and the future of ‘medical ethics’ by adopting a position that seeks to constructively engage empire, economics and apathy in relation to just health in South Africa.

Recent research by the Call42 group has shown that South African Christians experience that they are not adequately prepared or equipped for Christian living and discipleship in the world of work – here called the marketplace. This article has argued for the importance of a rediscovery of a theology of work that can empower and equip the church and individual Christians for ministry in the marketplace. The article traces why such a theological deficiency exists in the South African church by considering areas such as an inadequate theology of work and mission, a dualism between faith and work, and an unbalanced emphasis on the role of clergy and a lesser focus on the role of the laity in themissio Dei. Having considered these challenges to the mission and theological identity of the church, the article discusses the three general theological views of the church in South Africa as presented by Smit and adapted by Forster. It considers how the church could become an agent of mission and transformation in the marketplace in each of these three forms. The article comes to the conclusion that the church will need to revisit its missional theology, refocuses its efforts on broader society, and empowers and equips its members for ministry in the marketplace in order to be faithful in partnering with God in the missio Dei. Waar is die kerk op Maandag? Ontwaking van die kerk tot die teologie en praktyk vanbediening en sending in die markplein. Onlangse navorsing deur die Call42 groep het bevind dat Suid-Afrikaanse Christene ervaar dat hulle nie voldoende voorbereid en toegerus is vir die Christelike lewe en dissipelskap in die arbeidsmark - hier genoem die markplein – nie. Hierdie artikel poog om aan te toon dat ’n herontdekking van ’n teologie van werk belangrik is ten einde die kerk in die algemeen asook individuele Christene te bemagtig en toe te rus vir die bediening in die markplein. Hierdie artikel poog dus om die kwessie van die sodanige teologiese leemte in die Suid-Afrikaanse kerk na te vors. Terreine soos onvoldoende teologie van werk en sending word ondersoek, ’n dualisme tussen geloof en werk word uitgewys, en daar word aangetoon dat ’n oorspeling van die predikant se rol en ’n onderspeling van gewone kerklidmate se rol die kerk se betrokkenheid by die missio Dei benadeel. Met inagneming van hierdie uitdagings aan sending en die kerk se teologiese identiteit, bespreek die artikel drie algemene teologiese standpunte van die kerk in Suid Afrika, soos deur Smit aangebied en deur Forster aangepas. Die artikel besin hoe die kerk in elk van hierdie drie bestaansvorme ’n agent van sending en transformasie in die markplein kan wees. Die gevolgtrekking word gemaak dat die kerk die missionale of sendingteologie moet heroorweeg, opnuut moet fokus op die uitreik na die breër gemeenskap en lidmate vir bediening in die markplein moet bemagtig en toerus. Sodoende sal die kerk getrou wees aan die medewerking met God in die missio Dei.

What hope is there for South Africa? What role can the church play as a bearer of hope in South Africa? This article seeks to address these important questions. Firstly, it problematises the contemporary notion of hope in South Africa by showing that it is a complex theological and social concept. Next, a nuanced understanding of hope is presented by adopting a public theological methodology that brings dominant theological perspectives on eschatological hope into dialogue with the most recent statistics about the quality of life in South Africa from 1994, 2004 and 2014. The article proposes that the complexity of Christian hope necessitates an understanding of the present reality that is held in dynamic tension with the desired future – namely a present-futurist eschatology. Finally the article shows that from this vantage point the church, in its various forms and understandings, is able to be a bearer of Christian hope that can contribute towards shaping a better future for South Africa.

BackgroundThe cellulosome is a multi-enzyme machine, which plays a key role in the breakdown of plant cell walls in many anaerobic cellulose-degrading microorganisms. Ruminococcus flavefaciens FD-1, a major fiber-degrading bacterium present in the gut of herbivores, has the most intricate cellulosomal organization thus far described. Cellulosome complexes are assembled through high-affinity cohesin-dockerin interactions. More than two-hundred dockerin-containing proteins have been identified in the R. flavefaciens genome, yet the reason for the expansion of these crucial cellulosomal components is yet unknown.Methodology/principal findingsWe have explored the full spectrum of 222 dockerin-containing proteins potentially involved in the assembly of cellulosome-like complexes of R. flavefaciens. Bioinformatic analysis of the various dockerin modules showed distinctive conservation patterns within their two Ca(2+)-binding repeats and their flanking regions. Thus, we established the conceptual framework for six major groups of dockerin types, according to their unique sequence features. Within this framework, the modular architecture of the parent proteins, some of which are multi-functional proteins, was evaluated together with their gene expression levels. Specific dockerin types were found to be associated with selected groups of functional components, such as carbohydrate-binding modules, numerous peptidases, and/or carbohydrate-active enzymes. In addition, members of other dockerin groups were linked to structural proteins, e.g., cohesin-containing proteins, belonging to the scaffoldins.Conclusions/significanceThis report profiles the abundance and sequence diversity of the R. flavefaciens FD-1 dockerins, and provides the molecular basis for future understanding of the potential for a wide array of cohesin-dockerin specificities. Conserved differences between dockerins may be reflected in their stability, function or expression within the context of the parent protein, in response to their role in the rumen environment.

The answer to the question ‘who am I?’ is of fundamental importance to being human. Answers to this question have traditionally been sought from various disciplines and sources, which include empirical sources, such as biology and sociology, and phenomenological sources, such as psychology and religion. Although the approaches are varied, they have the notion of foundational truth, whether from an objective or subjective perspective, in common. The question of human identity that is the subject of this paper is germinated from the title of a book by WITS academic, Ivor Chipkin, entitled, Do South Africans exist? Nationalism, democracy and the identity of ‘the people’ (2007). This paper does not discuss Chipkin’s thoughts on nationalism and democracy; however, it considered the matter of human identity that is raised by his question. The approach taken by this paper on the notion of identity was significantly influenced by Brian McLaren’s postmodernist approach to Christian doctrine as outlined in his book A generous orthodoxy (2004) – a term coined by Yale Theologian, Hans Frei. The inadequacies of traditional approaches to human identity and consciousness that are based upon ‘foundational knowledge’ were thus considered. Both subjective and objective approaches to identity were touched upon, showing the weaknesses of these approaches in dealing with the complex nature of true human identity. The paper then presented an integrative framework for individual consciousness that is not static or ultimately quantifiable, but rather formulated in the process of mutual discovery that arises from a shared journey. The approach presented here drew strongly upon the groundbreaking work of Ken Wilber and Eugene de Quincey and related their ontological systems to the intersubjective approach to identity that can be found in the African philosophy and theology of ‘ubuntu’. This paper focused on how the ethics and theology of this indigenous knowledge system can contribute toward overcoming the impasse of validating individual identity in contemporary academic debates on human consciousness.

Abstract In everyday tasks, the choices we make incorporate complex trade-offs between conflicting factors that affect how we will achieve our goals. Previous experimental research has used dual-target visual search to determine how people flexibly adjust their behaviour and make choices that optimise their decisions. In this experiment, we leveraged a visual search task that incorporates complex trade-offs, and electroencephalography (EEG), to understand how neural mechanisms of selective attention contribute to choice behaviour in these tasks. On each trial, participants could choose to respond to the gap location on either of two possible targets. Each target was colour coded such that colour indicated which of the two had the easier gap discrimination. Orthogonally, we manipulated the set size of coloured distractors to modulate how efficiently each target could be found. As a result, optimised task performance required participants to trade-off conflicts between the ease of finding a target given the current set size, and the ease of making its associated gap discrimination. Our results confirm that participants are able to flexibly adjust their behaviour, and trade-off these two factors to maintain their response speed and accuracy. Additionally, the N2pc and SPCN components elicited by search displays could reliably predict the choice that participants would ultimately make on a given trial. These results suggest that initial attentional processes may help to determine the choice participants make, highlighting the central role that attention may play in optimising performance on complex tasks.

Abstract Background Laryngeal injury associated with traumatic or prolonged intubation may lead to voice, swallow, and airway complications. The interplay between inflammation and microbial population shifts induced by intubation may relate to clinical outcomes. The objective of this study was to investigate laryngeal mechanics, tissue inflammatory response, and local microbiome changes with laryngotracheal injury and localized delivery of therapeutics via drug-eluting endotracheal tube. Methods A simulated traumatic intubation injury was created in Yorkshire crossbreed swine under direct laryngoscopy. Endotracheal tubes electrospun with roxadustat or valacyclovir- loaded polycaprolactone (PCL) fibers were placed in the injured airway for 3, 7, or 14 days (n = 3 per group/time and ETT type). Vocal fold stiffness was then evaluated with normal indentation and laryngeal tissue sections were histologically examined. Immunohistochemistry and inflammatory marker profiling were conducted to evaluate the inflammatory response associated with injury and ETT placement. Additionally, ETT biofilm formation was visualized using scanning electron microscopy and micro-computed tomography, while changes in the airway microbiome were profiled through 16S rRNA sequencing. Results Laryngeal tissue with roxadustat ETT placement had increasing localized stiffness outcomes over time and histological assessment indicated minimal epithelial ulceration and fibrosis, while inflammation remained severe across all timepoints. In contrast, vocal fold tissue with valacyclovir ETT placement showed no significant changes in stiffness over time; histological analysis presented a reduction in epithelial ulceration and inflammation scores along with increased fibrosis observed at 14 days. Immunohistochemistry revealed a decline in M1 and M2 macrophage markers over time for both ETT types. Among the cytokines, IL-8 levels differed significantly between the roxadustat and valacyclovir ETT groups, while no other cytokines showed statistically significant differences. Additionally, increased biofilm formation was observed in the coated ETTs with notable alterations in microbiota distinctive to each ETT type and across time. Conclusion The injured and intubated airway resulted in increased laryngeal stiffness. Local inflammation and the type of therapeutic administered impacted the bacterial composition within the upper respiratory microbiome, which in turn mediated local tissue healing and recovery.

Abstract Members of the leucine rich repeat (LRR) and PDZ domain (LAP) protein family are essential for animal development and histogenesis. Densin-180, encoded by LRRC7, is the only LAP protein selectively expressed in neurons. Densin-180 is a postsynaptic scaffold at glutamatergic synapses, linking cytoskeletal elements with signalling proteins such as the α-subunit of Ca2+/calmodulin-dependent protein kinase II. We have previously observed an association between high impact variants in LRRC7 and Intellectual Disability; also three individual cases with variants in LRRC7 had been described. We identify here 33 individuals (one of them previously described) with a dominant neurodevelopmental disorder due to heterozygous missense or loss-of-function variants in LRRC7. The clinical spectrum involves intellectual disability, autism, ADHD, aggression and, in several cases, hyperphagia-associated obesity. A PDZ domain variant interferes with synaptic targeting of Densin-180 in primary cultured neurons. Using in vitro systems (two hybrid, BioID, coimmunoprecipitation of tagged proteins from 293T cells) we identified new candidate interaction partners for the LRR domain, including protein phosphatase 1 (PP1), and observed that variants in the LRR reduced binding to these proteins. We conclude that LRRC7 encodes a major determinant of intellectual development and behaviour.

Background: The stunting prevalence in Central Java Province remains high. Six key messages, i.e. iron tablet supplementation, healthy latrines, exclusive breastfeeding, Integrated Health Care Post (Posyandu) visit, hand washing, and Antenatal Care visit are the efforts to reduce the prevalence of stunting. Objectives: This study aimed to analyse the relationship between the behaviour of the six key messages and the stunting prevalence in four districts of Central Java Province. Methods: This cross-sectional study was conducted in Brebes Regency, Tegal Regency, Banyumas Regency, and Semarang City with 424 mothers who have under two-year-old children randomly selected as sample. Data were collected by interview using a structured questionnaire with the results grouped into attitude and practices categories. The body’s weight and length were measured by a digital scale and infantometer. Data were analysed using the chi-square test. Results: The findings indicated that 21.5% of children under two had stunting. Besides, behaviour regarding six key messages were mostly good. There was no association between mothers' iron tablet consumption behaviour and the nutritional status of under-two children. There was an association between maternal attitudes about exclusive breastfeeding and knowledge about hand washing with length for age index (p=0.004). There was also an association between antenatal care and Posyandu visits practice with weight for age index (p=0.004 and 0.018). There was a relationship between attitude regarding the use of latrines and the weight for length index (p=0.001). Conclusions: Only the key messages of exclusive breastfeeding attitude, antenatal care visit practice, Posyandu visit practice, and hand washing attitude were associated with the nutritional status of under-two children.

Abstract Developmental and epileptic encephalopathies (DEEs) feature altered brain development, developmental delay and seizures, with seizures exacerbating developmental delay. Here we identify a cohort with biallelic variants in DENND5A, encoding a membrane trafficking protein, and develop animal models with phenotypes like the human syndrome. We demonstrate that DENND5A interacts with Pals1/MUPP1, components of the Crumbs apical polarity complex required for symmetrical division of neural progenitor cells. Human induced pluripotent stem cells lacking DENND5A fail to undergo symmetric cell division with an inherent propensity to differentiate into neurons. These phenotypes result from misalignment of the mitotic spindle in apical neural progenitors. Cells lacking DENND5A orient away from the proliferative apical domain surrounding the ventricles, biasing daughter cells towards a more fate-committed state, ultimately shortening the period of neurogenesis. This study provides a mechanism for DENND5A-related DEE that may be generalizable to other developmental conditions and provides variant-specific clinical information for physicians and families.

Abstract Importance Most patients presenting with a hip fracture regardless of their comorbidities are surgically treated. A growing body of research states that a certain type of elderly patient could benefit more from a palliative approach. Objective Identify the patient who would benefit most from a palliative care approach instead of a surgery. Design Exploratory-matched retrospective cohort study between 2015 and 2021. Setting Single Level 1 Trauma Center. Participants There were 2240 hip fracture patients admitted to our institution between 2015 and 2021. Patients over 65 years old with intertrochanteric or femoral neck fractures could be included. A total of 129 patients opted for palliative care (Palliative Group = PG). This cohort was compared to a matched cohort (for age, sex and fracture type) who underwent surgery but died within three months of the procedure (Surgery Deceased Group = SDG) and another matched cohort who survived more than three months (Surgery Alive Group = SAG) following surgery. Main outcomes and measures Medical charts were reviewed for patient demographics, autonomy level, level of care, neurocognitive disorders (NCD), fracture type, in-hospital data and outpatient death within three months of admission. Analysis was performed through univariate and multivariate models with SAS OnDemand for Academics (alpha 0.05). Results Patients in the PG (n = 129) were 88.2 ± 7.2 years old, 71.3% were females, and 61.2% had a femoral neck fracture. Patients in the SDG (n = 95) and SAG (n = 107) were well matched. The PG differed from the SDG (n = 95) and SAG (n = 107) regarding NCD (85.3% vs. 57.9% vs. 36.4%, p

Abstract Background Serum creatinine is used as initial test to derive eGFR and confirmatory testing with serum cystatin C is recommended when creatinine‐based eGFR is considered less accurate due to deviant muscle mass. Low muscle mass is associated with increased risk of premature mortality. However, the associations of serum creatinine and cystatin C with muscle mass and mortality remain unclear and require further investigation to better inform clinical decision‐making. Methods We included 8437 community‐dwelling adults enrolled in the Dutch PREVEND study and 5033 in the US NHANES replication cohort. Associations of serum creatinine and/or cystatin C with muscle mass surrogates and mortality were quantified with linear and Cox proportional hazards regression, respectively. Missing observations in covariates were multiply imputed using Substantive Model Compatible Fully Conditional Specification. Results Mean (SD) age of PREVEND and NHANES participants (50% and 48% male) were 49.8 (12.6) and 48.7 (18.7) years, respectively. Median (Q1–Q3) serum creatinine and cystatin C were 71 (61–80) and 80 (62–88) μmol/L and 0.87 (0.78–0.98) and 0.91 (0.80–1.10) mg/L, respectively. Higher serum creatinine was associated with greater muscle mass, while serum cystatin C was not associated with muscle mass. Adjusting both markers for each other strengthened the positive relationship between serum creatinine and muscle mass and revealed an inverse association between serum cystatin C and muscle mass. In the PREVEND cohort, 1636 (19%) deaths were registered over a median follow‐up of 12.9 (5.8–16.3) years with a 10‐year mortality rate (95% CI) of 7.6% (7.1–8.2%). In the NHANES, 1273 (25%) deaths were registered over a median follow‐up of 17.9 (17.3–18.5) years with a 10‐year mortality rate of 13.8% (12.8–14.7%). Both markers were associated with increased mortality. Notably, when adjusted for each other, higher serum creatinine was associated with decreased mortality, while the association between serum cystatin C and increased mortality strengthened. The shapes of the associations in the PREVEND study and NHANES were almost identical. Conclusions The strong association between serum creatinine and muscle mass challenges its reliability as GFR marker, necessitating a more cautious approach in its clinical use. The minimal association between serum cystatin C and muscle mass supports its increased use as a more reliable alternative in routine clinical practice.

Abstract The contraction of CAG/CTG repeats is an attractive approach to correct the mutation that causes at least 15 neuromuscular and neurodegenerative diseases, including Huntington’s disease and Myotonic Dystrophy type 1. Contractions can be achieved in vivo using the Cas9 D10A nickase from Streptococcus pyogenes (SpCas9) using a single guide RNA (sgRNA) against the repeat tract. One hurdle on the path to the clinic is that SpCas9 is too large to be packaged together with its sgRNA into a single adeno-associated virus. Here we aimed to circumvent this problem using the smaller Cas9 orthologue, SlugCas9, and the Cas9 ancestor OgeuIscB. We found them to be ineffective in inducing contractions, despite their advertised PAM sequences being compatible with CAG/CTG repeats. Thus, we further developed smaller Cas9 hybrids, made of the PAM interacting domain of S. pyogenes and the catalytic domains of the smaller Cas9 orthologues. We also designed the cognate sgRNA hybrids using molecular dynamic simulations and binding energy calculations. We found that the four Cas9/sgRNA hybrid pairs tested in human cells failed to edit their target sequences. We conclude that in silico approaches can identify functional changes caused by point mutations but are not sufficient for designing larger scale complexes of Cas9/sgRNA hybrids.

Abstract Background Coronary heart disease (CHD) is the leading cause of deaths and disability worldwide. Cardiac rehabilitation (CR) effectively reduces the risk of future cardiac events and is strongly recommended in international clinical guidelines. However, CR program quality is highly variable with divergent data systems, which, when combined, potentially contribute to persistently low completion rates. The QUality Improvement in Cardiac Rehabilitation (QUICR) trial aims to determine whether a data-driven collaborative quality improvement intervention delivered at the program level over 12 months: (1) increases CR program completion in eligible patients with CHD (primary outcome), (2) reduces hospital admissions, emergency department presentations and deaths, and costs, (3) improves the proportion of patients receiving guideline-indicated CR according to national and international benchmarks, and (4) is feasible and sustainable for CR staff to implement routinely. Methods QUICR is a multi-centre, type-2, hybrid effectiveness-implementation cluster-randomized controlled trial (cRCT) with 12-month follow-up. Eligible CR programs (n = 40) and the individual patient data within them (n ~ 2,000) recruited from two Australian states (New South Wales and Victoria) are randomized 1:1 to the intervention (collaborative quality improvement intervention that uses data to identify and manage gaps in care) or control (usual care with data collection only). This sample size is required to achieve 80% power to detect a difference in completion rate of 22%. Outcomes will be assessed using intention-to-treat principles. Mixed-effects linear and logistic regression models accounting for clusters within allocated groupings will be applied to analyse primary and secondary outcomes. Discussion Addressing poor participation in CR by patients with CHD has been a longstanding challenge that needs innovative strategies to change the status-quo. This trial will harness the collaborative power of CR programs working simultaneously on common problem areas and using local data to drive performance. The use of data linkage for collection of outcomes offers an efficient way to evaluate this intervention and support the improvement of health service delivery. Ethics Primary ethical approval was obtained from the Northern Sydney Local Health District Human Research Ethics Committee (2023/ETH01093), along with site-specific governance approvals. Trial registration Australian New Zealand Clinical Trials Registry (ANZCTR) ACTRN12623001239651 (30/11/2023) ( https://anzctr.org.au/Trial/Registration/TrialReview.aspx?id=386540&isReview=true ).

Abstract Background Fuzzy cognitive mapping (FCM) is a graphic technique to describe causal understanding in a wide range of applications. This practice review summarises the experience of a group of participatory research specialists and trainees who used FCM to include stakeholder views in addressing health challenges. From a meeting of the research group, this practice review reports 25 experiences with FCM in nine countries between 2016 and 2023. Results The methods, challenges and adjustments focus on participatory research practice. FCM portrayed multiple sources of knowledge: stakeholder knowledge, systematic reviews of literature, and survey data. Methodological advances included techniques to contrast and combine maps from different sources using Bayesian procedures, protocols to enhance the quality of data collection, and tools to facilitate analysis. Summary graphs communicating FCM findings sacrificed detail but facilitated stakeholder discussion of the most important relationships. We used maps not as predictive models but to surface and share perspectives of how change could happen and to inform dialogue. Analysis included simple manual techniques and sophisticated computer-based solutions. A wide range of experience in initiating, drawing, analysing, and communicating the maps illustrates FCM flexibility for different contexts and skill bases. Conclusions A strong core procedure can contribute to more robust applications of the technique while adapting FCM for different research settings. Decision-making often involves choices between plausible interventions in a context of uncertainty and multiple possible answers to the same question. FCM offers systematic and traceable ways to document, contrast and sometimes to combine perspectives, incorporating stakeholder experience and causal models to inform decision-making. Different depths of FCM analysis open opportunities for applying the technique in skill-limited settings.

Abstract Strongly-interacting nanomagnetic arrays are ideal systems for exploring reconfigurable magnonics. They provide huge microstate spaces and integrated solutions for storage and neuromorphic computing alongside GHz functionality. These systems may be broadly assessed by their range of reliably accessible states and the strength of magnon coupling phenomena and nonlinearities. Increasingly, nanomagnetic systems are expanding into three-dimensional architectures. This has enhanced the range of available magnetic microstates and functional behaviours, but engineering control over 3D states and dynamics remains challenging. Here, we introduce a 3D magnonic metamaterial composed from multilayered artificial spin ice nanoarrays. Comprising two magnetic layers separated by a non-magnetic spacer, each nanoisland may assume four macrospin or vortex states per magnetic layer. This creates a system with a rich 16 N microstate space and intense static and dynamic dipolar magnetic coupling. The system exhibits a broad range of emergent phenomena driven by the strong inter-layer dipolar interaction, including ultrastrong magnon-magnon coupling with normalised coupling rates of $$\frac{\Delta f}{\nu }=0.57$$ Δ f ν = 0.57 , GHz mode shifts in zero applied field and chirality-control of magnetic vortex microstates with corresponding magnonic spectra.

Abstract Mutations in DNA damage response (DDR) factors are associated with human infertility, which affects up to 15% of the population. The DDR is required during germ cell development and meiosis. One pathway implicated in human fertility is DNA translesion synthesis (TLS), which allows replication impediments to be bypassed. We find that TLS is essential for pre-meiotic germ cell development in the embryo. Loss of the central TLS component, REV1, significantly inhibits the induction of human PGC-like cells (hPGCLCs). This is recapitulated in mice, where deficiencies in TLS initiation (Rev1 -/- or Pcna K164R/K164R ) or extension (Rev7 -/- ) result in a > 150-fold reduction in the number of primordial germ cells (PGCs) and complete sterility. In contrast, the absence of TLS does not impact the growth, function, or homeostasis of somatic tissues. Surprisingly, we find a complete failure in both activation of the germ cell transcriptional program and in DNA demethylation, a critical step in germline epigenetic reprogramming. Our findings show that for normal fertility, DNA repair is required not only for meiotic recombination but for progression through the earliest stages of germ cell development in mammals.

Background Patients with shock treated by emergency medical services (EMS) have high morbidity and mortality. Knowledge of prehospital factors predicting outcomes in patients with shock remains limited. We aimed to describe the prehospital predictors of mortality in patients with non-traumatic shock transported to hospital by EMS.Method This is a retrospective cohort study of consecutive ambulance attendances for non-traumatic shock in Victoria, Australia (January 2015–June 2019) linked with government-held administrative data (emergency, admissions and mortality records). Predictors of 30-day mortality were assessed using Cox proportional regressions. The primary outcome was 30-day all-cause mortality.Results Overall, 21 334 patients with non-traumatic shock (median age 69 years, 54.8% female) were successfully linked with state administrative records. Among this cohort, 9 149 (43%) patients died within 30-days. Compared with survivors, non-survivors had a longer median on-scene time: 60 (35–98) versus 30 (19–50), p

BackgroundFor the selective detection of thyroid-stimulating hormone receptor antibodies with stimulating properties (thyroid-stimulating immunoglobulins; TSI), a novel and rapid bioassay (Turbo TSI) has been introduced. We evaluate the clinical performance of Turbo TSI in Graves’ orbitopathy (GO) patients and compare it to a bridge-based TSI binding immunoassay and third generation TSH-R-binding inhibitory immunoglobulins (TBII) assay. Also, we investigate the association of Turbo TSI and TBII measurements with GO activity and severity, as well as response to intravenous methylprednisolone (IVMP), and compare results to previous findings on the bridge-based TSI binding immunoassay.MethodsTurbo TSI, TBII and bridge-based TSI binding immunoassay measurements were performed in biobank serum from 111 GO patients and control cases (healthy controls [HC; n=47], primary Sjögren’s disease [SD; n=10], systemic sclerosis [SSc; n= 10], systemic lupus erythematosus [SLE; n=10]). Clinical characteristics and response to treatment were retrospectively retrieved from GO patient files.ResultsTurbo TSI had the highest sensitivity (97.3%) and negative predictive value (96.1%), while bridge-based TSI binding immunoassay showed the highest specificity (100%) and positive predictive value (100%). Differentiating GO patients from control cases, receiver operating characteristic (ROC) analysis showed an area under the curve (AUC) of 98.5%, 95.7% and 99.8% for Turbo TSI, TBII and bridge-based TSI binding immunoassay, respectively. Turbo TSI (p

Contributed by scholars from diverse traditions and perspectives around the world, this article evidences both unifying and diversifying impulses in Christian ethics and moral thinking. Christian ethics is understood as both lived experience and academic study reflecting upon practice, deploying moral norms, engaging present-day issues, and more. Major topics include climate change, the body, peacemaking, and more. Consideration is also paid to the powerful the role of the Bible in the everyday lives and decision-making of Christian people. Persistent emphases include God’s bias to the poor and how God’s gift of the earth to all in common requires human beings to ensure that those things necessary for life are available to all, including future generations. Christian ethics is shown to be distinctive because it follows from belief in Jesus Christ, the incarnate Son of God, and capable of argumentation without direct reference to revelation. Together, and in a conscious effort to reflect the polyphony of Pentecost (Acts 2:4–6), the contributors find Christian ethics to be an academic discipline of both sorrow and hope.

Abstract Objectives With rapid advances in ultrasound‐guided procedures, there is an unmet need for echogenic phantoms with sufficient anatomical details for artificial intelligence and ultrasound‐guided device testing. We developed a method for creating neck phantoms for novel otolaryngology‐related device testing. To achieve accurate representation of the anatomy, we utilized CT scans and 3D printing technology to create customized agar molds, thus providing high‐fidelity yet cost‐effective tools. Methods Based on previous studies, the key components in our neck phantom include the cervical vertebrae, trachea, common carotid arteries, internal jugular veins, thyroid gland, and surrounding soft tissue. Open‐source image analysis software were employed to process CT data to generate high fidelity 3D models of the target structures. Resin molds were 3D printed and filled with various agar mixtures to mimic anatomical echogenicity. Results Following the method proposed, we successfully assembled the neck phantom which provided a detailed representation of the target structures. To evaluate the results, ultrasound data was collected on the phantom and living tissue and analyzed with ImageJ. We were able to demonstrate echogenicity comparable to that of living tissue. Conclusion The proposed method for building neck phantoms with detailed anatomical features offers a valuable, detailed, low‐cost tool for medical training and device testing in otolaryngology, particularly for novel devices that involve artificial intelligence (AI) guidance and robotic‐based needle insertion. Additional anatomical refinements and validation studies could further enhance the consistency and accuracy, thus paving the way for future advancements in ultrasound training and research, and ultimately benefiting patient care and safety.

Summary: Reactive changes of glial cells during neuroinflammation impact brain disorders and disease progression. Elucidating the mechanisms that control reactive gliosis may help us to understand brain pathophysiology and improve outcomes. Here, we report that adult ablation of autism spectrum disorder (ASD)-associated CHD8 in astrocytes attenuates reactive gliosis via remodeling chromatin accessibility, changing gene expression. Conditional Chd8 deletion in astrocytes, but not microglia, suppresses reactive gliosis by impeding astrocyte proliferation and morphological elaboration. Astrocyte Chd8 ablation alleviates lipopolysaccharide-induced neuroinflammation and septic-associated hypothermia in mice. Astrocytic CHD8 plays an important role in neuroinflammation by altering the chromatin landscape, regulating metabolic and lipid-associated pathways, and astrocyte-microglia crosstalk. Moreover, we show that reactive gliosis can be directly mitigated in vivo using an adeno-associated virus (AAV)-mediated Chd8 gene editing strategy. These findings uncover a role of ASD-associated CHD8 in the adult brain, which may warrant future exploration of targeting chromatin remodelers in reactive gliosis and neuroinflammation in injury and neurological diseases.

Summary: Background: It is uncertain which biological features underpin the response of rectal cancer (RC) to radiotherapy. No biomarker is currently in clinical use to select patients for treatment modifications. Methods: We identified two cohorts of patients (total N = 249) with RC treated with neoadjuvant radiotherapy (45Gy/25) plus fluoropyrimidine. This discovery set included 57 cases with pathological complete response (pCR) to chemoradiotherapy (23%). Pre-treatment cancer biopsies were assessed using transcriptome-wide mRNA expression and targeted DNA sequencing for copy number and driver mutations. Biological candidate and machine learning (ML) approaches were used to identify predictors of pCR to radiotherapy independent of tumour stage. Findings were assessed in 107 cases from an independent validation set (GSE87211). Findings: Three gene expression sets showed significant independent associations with pCR: Fibroblast-TGFβ Response Signature (F-TBRS) with radioresistance; and cytotoxic lymphocyte (CL) expression signature and consensus molecular subtype CMS1 with radiosensitivity. These associations were replicated in the validation cohort. In parallel, a gradient boosting machine model comprising the expression of 33 genes generated in the discovery cohort showed high performance in GSE87211 with 90% sensitivity, 86% specificity. Biological and ML signatures indicated similar mechanisms underlying radiation response, and showed better AUC and p-values than published transcriptomic signatures of radiation response in RC. Interpretation: RCs responding completely to chemoradiotherapy (CRT) have biological characteristics of immune response and absence of immune inhibitory TGFβ signalling. These tumours may be identified with a potential biomarker based on a 33 gene expression signature. This could help select patients likely to respond to treatment with a primary radiotherapy approach as for anal cancer. Conversely, those with predicted radioresistance may be candidates for clinical trials evaluating addition of immune-oncology agents and stromal TGFβ signalling inhibition. Funding: The Stratification in Colorectal Cancer Consortium (S:CORT) was funded by the Medical Research Council and Cancer Research UK (MR/M016587/1).

Abstract Background Brain-computer interface (BCI) technology offers children with quadriplegic cerebral palsy unique opportunities for communication, environmental exploration, learning, and game play. Research in adults demonstrates a negative impact of fatigue on BCI enjoyment, while effects on BCI performance are variable. To date, there have been no pediatric studies of BCI fatigue. The purpose of this study was to assess the effects of two different BCI paradigms, motor imagery and visual P300, on the development of self-reported fatigue and an electroencephalography (EEG) biomarker of fatigue in typically developing children. Methods Thirty-seven typically-developing school-aged children were recruited to a prospective, crossover study. Participants attended three sessions: (A) motor imagery-BCI, (B) visual P300-BCI, and (C) video viewing (control). The motor imagery task involved an imagined left- or right-hand squeeze. The P300 task involved attending to one square on a 3 × 3 grid during a random single flash sequence. Each paradigm had respective calibration periods and a similar visual counting game. Primary outcomes were self-reported fatigue and the power of the EEG alpha band both collected during resting-state periods pre- and post-task. Self-reported fatigue was measured using a 10-point visual analog scale. EEG alpha band power was calculated as the integrated power spectral density from 8 to 12 Hz of the EEG spectrum. Results Thirty-two children completed the protocol (age range 7–16, 63% female). Self-reported fatigue and EEG alpha band power increased across all sessions (F (1,155) = 33.9, p

ABSTRAK Perkembangan teknologi yang semakin pesat memudahkan masyarakat dalam berbagai aspek kehidupan. Media sosial adalah hasil dari perkembangan teknologi yang digunakan dalam kegiatan sehari-hari. Akses media sosial yang mudah dengan jangkauan yang luas dapat membantu penggunanya untuk mendapatkan perhatian publik yang akan mendukung kegiatan pemasaran. Maka dari itu, penelitian ini memiliki tujuan untuk menganalisa pengaruh dari Attractiveness, Trustworthiness, dan Expertise Endorser dalam terhadap niat beli konsumen di media sosial, dengan mediasi Brand Image dan Attitude. Metode penelitian yang digunakan adalah metode penelitian kuantitatif dengan menggunakan teknik purposive sampling. Populasi dari penelitian ini adalah masyarakat Batam yang memiliki akun media sosial. Jumlah responden yang dalam penelitian ini adalah sebanyak 275 responden dengan jumlah pertanyaan 24. Analisa yang digunakan dalam penelitian ini adalah PLS-SEM. Hasil dari penelitian ini menunjukkan bahwa variabel attractiveness dan expertise memiliki pengaruh positif baik secara langsung maupun tidak langsung yang dimediasikan oleh brand image dan attitude terhadap purchase intention, variabel trustworthiness juga memiliki pengaruh positif terhadap purchase intention baik secara langsung dan tidak langsung yang dimediasikan oleh attitude, namun tidak dengan yang dimediasikan oleh brand image. Kata Kunci: Teknologi, Media Sosial, Endorser, Citra Merek, Sikap. ABSTRACT The rapid development of technology makes various life aspects easier. Social media is the results of technology developments used in daily activities. Easy access to social media with a wide reach can help users to get public attention which will support marketing activities. Therefore, this research aims to analyze Attractiveness, Trustworthiness, and Expertise of an Endorser influence in determining consumer purchasing intentions on social media, with the mediation of Brand Image and Attitude. This research uses quantitative research methods with purposive sampling technique. This study population that used are the social media user in Batam. The number used respondents from this research was 275 respondents with 24 questions. The analysis used in this research was PLS-SEM. The research result show that attractiveness and expertise variables have a positive effect directly and indirectly that mediated by brand image and attitude, on purchase intention, the trustworthiness variable also has a positive effect on purchase intention, directly and indirectly that mediated by attitude, but didn’t show positive effect that mediated by brand image. Keywords: Technology, Social Media, Endorser, Brand Image, Attitude

Abstract Child maltreatment is a global concern that profoundly affects individuals throughout their lives. This study investigated the relationships between various forms of child maltreatment and behavior problems involving internalization and externalization during adolescence. Data obtained from a diverse sample of 1802 Canadians aged 14–18 years was used to examine the mediating role of alexithymia—a difficulty in recognizing and expressing emotions—in these associations. Results indicated that adolescents who experienced sexual abuse, emotional abuse, and exposure to intimate partner violence (IPV) in their childhood exhibited higher levels of alexithymia, which was correlated with elevated levels of both internalizing and externalizing problems. Physical abuse and parental neglect were only associated with externalizing problems. Gender differences also emerged, with gender-diverse adolescents reporting a higher prevalence of maltreatment, alexithymia, and behavior problems compared with their peers. However, alexithymia’s mediating role was consistent across genders. Overall, this study highlights the intricate relationships between child maltreatment, alexithymia, and adolescent behavior problems. The findings of this study how different forms of child maltreatment significantly shape behavioral outcomes and indicate the importance of interventions in enhancing emotional awareness and expression in adolescents with a childhood history of maltreatment.

Abstract In-sensor computing could become a fundamentally new approach to the deployment of machine learning in small devices that must operate securely with limited energy resources, such as wearable medical devices and devices for the Internet of Things. Progress in this field has been slowed by the difficulty to find appropriate computing devices that operate using physical degrees of freedom that can be coupled directly to degrees of freedom that perform sensing. Here we leverage reservoir computing as a natural framework to do machine learning with the degrees of freedom of a physical system, to show that a micro-electromechanical system can implement computing and the sensing of accelerations by coupling the displacement of suspended microstructures. We present a complete wearable system that can be attached to the foot to identify the gait patterns of human subjects in real-time. The computing efficiency and the power consumption of this in-sensor computing system is then compared to a conventional system with a separate sensor and digital computer. For similar computing capabilities, a much better power efficiency can be expected for the highly-integrated in-sensor computing devices, thus providing a path for the ubiquitous deployment of machine learning in edge computing devices.