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Purpose: Epidemiological studies examining the association between circulating micronutrients and the risk of hypertensive disorders during pregnancy (HDP) have produced inconsistent results. Therefore, we conducted a Mendelian randomization (MR) analysis to evaluate the potential causal relationship between micronutrients and HDP. Methods: Nine micronutrients (beta-carotene, vitamin B6, vitamin B12, calcium, zinc, selenium, copper, folate, and phosphorus) were selected as the exposure factors. Summary data for gestational hypertension (14,727 cases and 196,143 controls) and preeclampsia/eclampsia (7212 cases and 174,266 controls) were extracted from the FinnGen consortium. The MR analysis employed the inverse variance weighted method and conducted a range of sensitivity analyses. Results: The inverse variance weighted method indicated no significant causal relationship between nine genetically predicted micronutrient concentrations and gestational hypertension, as well as preeclampsia/eclampsia. Sensitivity analyses suggested the absence of pleiotropy. Conclusion: There is no strong evidence to support the causation between circulating micronutrients and hypertensive disorder during pregnancy. [ABSTRACT FROM AUTHOR]

Background: Z-DNA binding protein 1 (ZBP1) is a nucleic acid sensor that is involved in multiple inflammatory diseases, but whether and how it contributes to osteoarthritis (OA) are unclear. Methods: Cartilage tissues were harvested from patients with OA and a murine model of OA to evaluate ZBP1 expression. Subsequently, the functional role and mechanism of ZBP1 were examined in primary chondrocytes, and the role of ZBP1 in OA was explored in mouse models. Results: We showed the upregulation of ZBP1 in articular cartilage originating from OA patients and mice with OA after destabilization of the medial meniscus (DMM) surgery. Specifically, knockdown of ZBP1 alleviated chondrocyte damage and protected mice from DMM-induced OA. Mechanistically, tumor necrosis factor alpha induced ZBP1 overexpression in an interferon regulatory factor 1 (IRF1)-dependent manner and elicited the activation of ZBP1 via mitochondrial DNA (mtDNA) release and ZBP1 binding. The upregulated and activated ZBP1 could interact with receptor-interacting protein kinase 1 and activate the transforming growth factor-beta-activated kinase 1-NF-κB signaling pathway, which led to chondrocyte inflammation and extracellular matrix degradation. Moreover, inhibition of the mtDNA-IRF1-ZBP1 axis with Cyclosporine A, a blocker of mtDNA release, could delay the progression of DMM-induced OA. Conclusions: Our data revealed the pathological role of the mtDNA-IRF1-ZBP1 axis in OA chondrocytes, suggesting that inhibition of this axis could be a viable therapeutic approach for OA. [ABSTRACT FROM AUTHOR]

The objective of this study is to investigate the impact of emotional design and sustainable product design on user experience and satisfaction. Additionally, the study aims to optimize the combination efficiency and material selection methods of these two design approaches, extracting optimization parameters based on emotional and sustainable product design principles. By analyzing user satisfaction through questionnaires, various product indicators were examined. The study ultimately establishes a BP neural network optimization model by analyzing the design elements of different products and the satisfaction levels of various indicators. This BP neural network design optimization model, integrating emotional and sustainable design, demonstrates an accuracy rate of over 95% in predicting user satisfaction for different design schemes. Upon examining the emotional design and sustainable design parameter tables following data standardization, and analyzing various product models, it was concluded that the rate of change in each design parameter significantly impacts the satisfaction of each product indicator, highlighting the importance of these parameters in product design optimization. [ABSTRACT FROM AUTHOR]

A simple and flexible mass balance approach was applied to observations of XCH4 from TROPOMI to estimate CH4 emissions over Shanxi Province, including the impacts of advective transport, pressure transport, and atmospheric diffusion. High-frequency eddy-covariance flux observations were used to constrain the driving terms of the mass balance equation. This equation was then used to calculate day-to-day and 5 km × 5 km grided CH4 emissions from May 2018 to July 2022 based on TROPOMI RPRO column CH4 observations. The Shanxi-wide emissions of CH4, 126 ± 58.8 ug/m2/s, shows a fat tail distribution and high variability on a daily time scale (the 90th percentile is 2.14 times the mean and 2.74 times the median). As the number of days in the rolling average increases, the change in the variation decreases to 128 ± 35.7 ug/m2/s at 10-day, 128 ± 19.8 ug/m2/s at 30-day and 127 ± 13.9 ug/m2/s at 90-day. The range of values of the annual mean emissions on coal mine grids within Shanxi for the years 2018 to 2022 was 122 ± 58.2, 131 ± 71.2, 111 ± 63.6, 129 ± 87.1, and 138 ± 63.4 ug/m2/s, respectively. The 5-year average emissions from TROPOMI are 131 ± 68.0 ug/m2/s versus 125 ± 94.6 ug/m2/s on the grids where the EDGAR bottom-up database also has data, indicating that those pixels with mines dominate the overall emissions in terms of both magnitude and variability. The results show that high-frequency observation-based campaigns can produce a less biased result in terms of both the spatial and temporal distribution of CH4 emissions as compared with approaches using either low-frequency data or bottom-up databases, that coal mines dominate the sources of CH4 in Shanxi, and that the observed fat tail distribution can be accounted for using this approach. [ABSTRACT FROM AUTHOR]

Background: To compare the repeatability and reproducibility of corneal and corneal epithelial thickness mapping using anterior segment optical coherence tomography (AS-OCT) according to tear film break-up time (TBUT). Methods: The included eyes were divided into three subgroups according to TBUT (group 1: TBUT ≤ 5 s, group 2: 5 s < TBUT ≤ 10 s, and group 3: TBUT > 10 s). All eyes were imaged separately thrice by two operators to obtain the thickness maps (TMs) of the cornea and corneal epithelium based on spatial zones encompassing a 9-mm-diameter area. Each TM consisted of 25 areas. Intraoperator (repeatability) and interoperator (reproducibility) standard deviations (Sws), coefficients of variation (CoVs), and intraclass correlation coefficients (ICCs) among the tests were calculated and compared in all the areas. Results: Altogether, 132 eyes of 67 subjects were included (50, 47, and 35 eyes in groups 1, 2, and 3; respectively). The ICCs of corneal epithelial thickness and corneal thickness were > 0.75 in most of the areas. Pairwise comparisons showed that AS-OCT exhibited lower repeatability in group 1 than in groups 2 and 3 (P < 0.05). However groups 2 and 3 showed similar results. Sws and CoVs of corneal epithelial thickness exhibited no significant interoperator differences. While no significant differences were observed in corneal thickness in most of the areas. Conclusions: TBUT significantly influences the repeatability of corneal and corneal epithelial thickness measurements. Poor tear film stability requires careful evaluation of corneal epithelial thickness. [ABSTRACT FROM AUTHOR]

Background: Age-related blepharoptosis, or ptosis, affects vision and appearance. Associations with age, gender, BMI, and diabetes have been explored, but the link to blood lipids remains unclear. The impact on refraction also lacks consensus. This study addresses gaps by investigating ptosis prevalence and factors in a representative Chinese population, aiming for a comprehensive understanding. Methods: A cross-sectional study was conducted among individuals aged 50 and above who were willing to participate in comprehensive systemic check-ups, behavioral questionnaires, and ophthalmic examinations at Yaoxi Community Health Center in Wenzhou City, Zhejiang Province. Results: The prevalence of blepharoptosis among the elderly participants at this health center was 27.16%. Individuals with blepharoptosis tended to be older, male, exhibited slightly higher body mass index, wider waist circumference, engaged in lower exercise frequency, and had a higher prevalence of hypertension, diabetes, and with-the-rule astigmatism compared to their counterparts without these conditions. Adjusting for all other confounding variables, older age, being male, higher fasting plasma glucose (FPG), and lower exercise frequency displayed statistically significant relationships with blepharoptosis. After examining the distribution of blepharoptosis degrees within relevant factor subgroups, we noted a higher prevalence of severe ptosis in subgroups associated with older age, male gender, higher FPG, and against-the-rule astigmatism. Conclusion: The notable associations with age, gender, FPG, and exercise level suggest a multifactorial etiology for blepharoptosis. The observed link between with-the-rule astigmatism and blepharoptosis implies a potential contributory role in the refractive aspect of blepharoptosis. Level of Evidence IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. [ABSTRACT FROM AUTHOR]

Quantifying the mechanical properties of the cornea can provide valuable insights into the occurrence and progression of keratoconus, as well as the effectiveness of corneal crosslinking surgery. This study presents a non‐contact and non‐invasive wave‐based optical coherence elastography system that utilizes air‐pulse stimulation to create a two‐dimensional map of corneal elasticity. Homogeneous and dual concentration phantoms were measured with the sampling of 25 × 25 points over a 6.6 × 6.6 mm2 area, to verify the measurement capability for elastic mapping and the spatial resolution (0.91 mm). The velocity of elastic waves distribution of porcine corneas before and after corneal crosslinking surgery were further mapped, showing a significant change in biomechanics in crosslinked region. This system features non‐invasiveness and high resolution, holding great potential for application in ophthalmic clinics. [ABSTRACT FROM AUTHOR]

Reducing methane emissions in the oil and gas industry is a top priority for the current international community in addressing climate change. Methane emissions from the energy sector exhibit strong temporal variability and ground monitoring networks can provide time-continuous measurements of methane concentrations, enabling the rapid detection of sudden methane leaks in the oil and gas industry. Therefore, identifying specific locations within oil fields to establish a cost-effective and reliable methane monitoring ground network is an urgent and significant task. In response to this challenge, this study proposes a technical workflow that, utilizing emission inventories, atmospheric transport models, and intelligent computing techniques, automatically determines the optimal locations for monitoring stations based on the input quantity of monitoring sites. This methodology can automatically and quantitatively assess the observational effectiveness of the monitoring network. The effectiveness of the proposed technical workflow is demonstrated using the Shengli Oilfield, the second-largest oil and gas extraction base in China, as a case study. We found that the Genetic Algorithm can help find the optimum locations effectively. Besides, the overall observation effectiveness grew from 1.7 to 5.6 when the number of site increased from 1 to 9. However, the growth decreased with the increasing site number. Such a technology can assist the oil and gas industry in better monitoring methane emissions resulting from oil and gas extraction. [ABSTRACT FROM AUTHOR]

Background: Pudendal neuralgia is a chronic and debilitating condition. Its prevalence ranges from 5 to 26%. Currently, therapeutic approaches to treat pudendal neuralgia include patient education, medication management, psychological and physical therapy, and procedural interventions, such as nerve block, trigger point injections, and surgery. Drug therapy has a limited effect on pain relief. A pudendal nerve block may cause a significant decrease in pain scores for a short time; however, its efficacy significantly decreases over time. In contrast, pudendal nerve pulsed radiofrequency can provide pain relief for 3 months, and ganglion impar block has been widely used for treating chronic perineal pain and chronic coccygodynia. This study aimed to determine the efficacy and safety of monotherapy (pudendal nerve pulsed radiofrequency) and combination therapy (pudendal nerve pulsed radiofrequency plus ganglion impar block) in patients with pudendal neuralgia. Methods: This randomized, controlled clinical trial will include 84 patients with pudendal neuralgia who failed to respond to drug or physical therapy. Patients will be randomly assigned into one of the two groups: mono or combined treatment groups. The primary outcome will be a change in pain intensity measured using the visual analog scale. The secondary outcomes will include a Self-Rating Anxiety Scale score, Self-Rating Depression Scale score, the use of oral analgesics, the Medical Outcomes Study Health Survey Short Form-36 Item score, and the occurrence of adverse effects. The study results will be analyzed using intention-to-treat and per-protocol analyses. Primary and secondary outcomes will be evaluated between the mono and combined treatment groups. Subgroup analyses will be conducted based on the initial ailment, age, and baseline pain intensity. The safety of the treatment will be assessed by monitoring adverse events, which will be compared between the two groups. Discussion: This study protocol describes a randomized, controlled clinical trial to determine the efficacy and safety of mono and combination therapies in patients with pudendal neuralgia. The study results will provide valuable information on the potential benefits of this combination therapy and contribute to the development of more effective and safer treatments for patients with pudendal neuralgia. Trial registration: Chinese Clinical Trial Registry (ChiCTR2200061800). [ABSTRACT FROM AUTHOR]

Introduction: Nitrososphaeria, formerly known as Thaumarchaeota, constitute a diverse and widespread group of ammonia-oxidizing archaea (AOA) inhabiting ubiquitously in marine and terrestrial environments, playing a pivotal role in global nitrogen cycling. Despite their importance in Earth's ecosystems, the cellular organization of AOA remains largely unexplored, leading to a significant unanswered question of how the machinery of these organisms underpins metabolic functions. Methods: In this study, we combined spherical-chromatic-aberration-corrected cryo-electron tomography (cryo-ET), scanning transmission electron microscopy (STEM), and energy dispersive X-ray spectroscopy (EDS) to unveil the cellular organization and elemental composition of Nitrosopumilus maritimus SCM1, a representative member of marine Nitrososphaeria. Results and Discussion: Our tomograms show the native ultrastructural morphology of SCM1 and one to several dense storage granules in the cytoplasm. STEM-EDS analysis identifies two types of storage granules: one type is possibly composed of polyphosphate and the other polyhydroxyalkanoate. With precise measurements using cryo-ET, we observed low quantity and density of ribosomes in SCM1 cells, which are in alignment with the documented slow growth of AOA in laboratory cultures. Collectively, these findings provide visual evidence supporting the resilience of AOA in the vast oligotrophic marine environment. [ABSTRACT FROM AUTHOR]

Due to policy changes in the context of COVID-19 pandemic, online teaching has become the main form of class in many Chinese universities. Flipped classroom has been widely used in other disciplines, but there is a dearth of evidence available about the use in online teaching of emergency medicine. This study aimed to develop a flipped classroom for online emergency medicine teaching and evaluate its effectiveness by comparing it with traditional lecture-based online teaching. A total of 62 clinical medical undergraduates from Jinan University participated in this study from September to December in 2022. An online flipped classroom approach was developed (FC group, n = 31). Traditional lecture-based online teaching was applied as a contrast (LBT group, n = 31). The undergraduates completed examinations and questionnaires at the end of the course. A course experience questionnaire and course examination score were used to evaluate the effectiveness of the flipped classroom approach. Regarding the five dimensions of the course experience questionnaire, the scores for good teaching (3.47 ± 0.50 vs. 2.34 ± 0.48, p <.001), appropriate assessment (3.31 ± 0.68 vs. 2.95 ± 0.71, p =.043) and generic skills (3.16 ± 0.60 vs. 2.72 ± 0.39, p <.001) were higher for the FC group than for the LBT group. There was no significant difference between the two groups in clear goals and standards, and appropriate workload. The undergraduates in the FC group showed significantly higher overall satisfaction than those in the LBT group (3.52 ± 0.1.03 vs. 2.87 ± 0.92, p =.012). The examination scores (77.936 ± 11.573 vs. 70.484 ± 7.434, p <.001), especially the scores for questions related to case analysis (33.032 ± 5.363 vs. 26.968 ± 7.657, p <.001), were significantly higher in the FC group than in the LBT group. The flipped classroom for online teaching was efficient in improving undergraduates' emergency medical academic performance and promoting the development of clinical case analysis ability. These findings provide an alternative flipped classroom approach for online teaching of emergency medicine. [ABSTRACT FROM AUTHOR]

Current research has increasingly focused on the preventive role of individual legal socialization in crime. The socialization of legal emotions is an important part of legal socialization. Building upon existing literature, this study, conducted through two sub-studies, investigated the influencing factors of legal emotions in N mainland Chinese university students and the mechanisms through which legal emotions impact aggressive behavior. In study 1, the results indicated that mother-child attachment, innovation spirit, and positive emotional expression positively predicted positive legal emotion, while mother-child attachment, dependency dimension in adult attachment, and positive emotional expression negatively predicted negative legal emotions. The anxiety dimension in adult attachment and negative emotional expression positively predicted negative legal emotions. In study 2, Positive legal emotion among university students could directly negatively predict aggressive behavior or exert influence through social alienation. Negative legal emotions could not only directly positively predict aggressive behavior but also partly affect it through social alienation. In summary, our study not only identified factors that influence legal emotions, but also found that legal emotions have an impact on aggressive behavior directly or indirectly through social alienation. Our research findings have significant implications for cultivating positive legal emotion in university students and curbing aggressive behavior. This can be achieved by promoting the legal socialization of university students and ultimately contributing to crime prevention. [ABSTRACT FROM AUTHOR]

China stands out as a major contributor to anthropogenic methane (CH 4) emissions, with coal mine methane (CMM) playing a crucial role. To control and reduce CH 4 emissions, China has made a dedicated commitment and formulated an ambitious mitigation plan. To verify the progress made, the consistent acquisition of independent CH 4 emission data is required. This paper aims to implement a wind-assigned anomaly method for the precise determination of regional-scale CMM emissions within the coal-rich Shanxi province. We use the TROPOspheric Monitoring Instrument (TROPOMI) CH 4 observations from May 2018 to May 2023, coupled with ERA5 wind and a bottom-up inventory dataset based on the IPCC (Intergovernmental Panel on Climate Change) Tier 2 approach covering the Changzhi, Jincheng, and Yangquan regions of the Shanxi province. The derived emission strengths are 8.4 × 10 26 molec. s -1 (0.706 Tg yr -1 , ± 25 %), 1.4 × 10 27 molec. s -1 (1.176 Tg yr -1 , ± 20 %), and 4.9 × 10 26 molec. s -1 (0.412 Tg yr -1 , ± 21 %), respectively. Our results exhibit biases of - 18 %, 8 %, and 14 %, respectively, when compared to the IPCC Tier 2 bottom-up inventory. Larger discrepancies are found when comparing the estimates to the Copernicus Atmosphere Monitoring Service global anthropogenic emissions (CAMS-GLOB-ANT) and Emissions Database for Global Atmospheric Research (EDGARv7.0) inventories (64 %–176 %), suggesting that the two inventories may be overestimating CH 4 emissions from the studied coal mining regions. Our estimates provide a comprehensive characterization of the regions within the Shanxi province, contribute to the validation of emission inventories, and provide additional insights into CMM emission mitigation. [ABSTRACT FROM AUTHOR]

The Qinghai-Tibetan Plateau (QTP), the source of many major Asian rivers, is sensitive to climate change, affecting billions of people's livelihoods across Asia. Here, we developed high-resolution projections of precipitation and daily maximum/minimum temperatures at 0.1° spatial resolution over the QTP. The projections are based on the output from seven global climate models (GCMs) from the Coupled Model Intercomparison Project Phase 6 (CMIP6) for historical (1979–2013) and projected (2015–2100) climates across four scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5). An updated nonstationary cumulative distribution function matching method (called CNCDFm) was used to remove model systemic bias. We verify the necessity of taking into account altitude in downscaling processes and the validity of nonstationary bias correction. Compared to the historical period, the climate in the QTP in the 21st century is warmer (1.2–5.1 °C, for maximum surface temperature) and wetter (3.9–26.8%) according to the corrected GCM projection. For precipitation, the Indus River (IDR), Tarim River (TMR), Inner of Qiangtang Basin (IQTB), Yarlung Zangbo (YLZBR), and Qaidam Basin (QDB) showed growth well above the global average across high radiative forcing scenarios, which could have a profound impact on the regional hydrological cycle. However, there is great uncertainty in precipitation prediction, which is demonstrated by a very low signal-to-noise ratio (SNR) and a large difference between Bayesian model averaging (BMA) and multi-model averages (MMAs). This bias-corrected dataset is available for climate change impact research in the QTP at the subregion scale. [ABSTRACT FROM AUTHOR]

Actual evapotranspiration constitutes a vital component of the exchange of energy and water vapour between the soil‐vegetation and atmospheric systems on terrestrial terrain. Nevertheless, the Tibetan Plateau, owing to its austere environmental conditions, harbours a scarcity of terrestrial monitoring stations. This circumstance presents a formidable challenge in attaining precise estimations of actual evapotranspiration. The complementary relationship method is a potential approach because it requires only routine meteorological data to estimate actual evapotranspiration on a regional or global scale. However, the suitability of the complementary relationship model across diverse ecosystems on the Tibetan Plateau necessitates further investigation. In this study, we scrutinized the simulation of daily and monthly actual evapotranspiration across 18 observation sites spanning eight distinct land use categories on the Tibetan Plateau. We employed the polynomial generalized complementary function introduced by Brutsaert (B2015), alongside its enhanced rendition proposed by Szilagyi (S2017) and Crago (C2018). The outcomes reveal that all three models adeptly replicate the fluctuations in actual evapotranspiration, irrespective of land use category or temporal scale—whether daily or monthly. This is true regardless of whether original or calibrated parameter values are applied. However, there exist significant variations in the performance of these models. In general, the C2018 model demonstrates superior performance across most ecosystems when original parameters are employed. Following parameter calibration, the simulation efficacy of the models experienced marked enhancement. Post parameter calibration, the B2015 model outperforms the other two models notably in desert and wetland environments. Furthermore, the simulation outputs from all three models display heightened sensitivity to parameter α, particularly in the context of the C2018 and S2017 models. These findings suggest that accurate estimation of parameter values is critical to improving the accuracy of estimating actual evapotranspiration. Calibrated parameter values, contingent on a fusion of vegetation, meteorology and surface roughness, exhibit variability across diverse ecosystems. [ABSTRACT FROM AUTHOR]

Purpose: To explore changes in corneal epithelial thickness (CET) after femtosecond laser–assisted laser in situ keratomileusis in patients with high astigmatism. Methods: CET was measured at every intersection of the concentric circles and specific axes using AngioVue optical coherence tomography (Angio-OCT) preoperatively and 1 month postoperatively. The average thickness of corneal central, paracentral, and peripheral regions was the mean of the points within the central 2, 2 to 5, and 5 to 7 mm areas, respectively. Correlation analysis was performed to investigate the association between CET along different axes and other preoperative and postoperative parameters. Results: Forty-two eyes of 28 patients were included. CET along the astigmatic (K1) and perpendicular (K2) axes in the central and paracentral areas increased (P <.001), whereas that along the K2 axis decreased in the peripheral area 1 month postoperatively (P =.001). The amount of CET change in the peripheral area between the K1 and K2 axes was significantly different (P <.001). In the central area, the change in CET along the K2 axis was positively correlated with ablation depth (r = 0.315, P =.042) and negatively with refractive power after surgery (r = −0.347, P =.024). In the peripheral area, the changes in CET along both K1 and K2 axes were negatively correlated with ablation depth (r = −0.431, P =.004; r = −0.387, P =.011, respectively). Conclusions: Epithelial modeling differed between the different astigmatism axes after refractive surgery. The compensatory response of the corneal epithelium is more pronounced along the steeper axis. [J Refract Surg. 2024;40(4):e239–e244.] [ABSTRACT FROM AUTHOR]

PDL1 blockade therapy holds great promise in cancer immunotherapy. Ultrasound imaging of PDL1 expression in the tumor is of great importance in predicting the therapeutic efficacy. As a proof‐of‐concept study, a novel ultrasound contrast agent has been innovated here to image and block PDL1 in the tumor tissue. Briefly, extracellular vesicles (EVs) are engineered to display truncated PD1 (tPD1) on the surface to bind PDL1 with high affinity by fusion to EV‐abundant transmembrane protein PTGFRN. The engineered EVs are then encapsulated with Ca(HCO3)2 via electroporation and designated as Gp‐EVtPD1, which would recognize PDL1 highly expressed cells and produce gas in the endosomes and lysosomes. On the one hand, the echogenic signal intensity correlates well with the PDL1 expression and immune response inhibition in the tumor. On the other hand, during the trajectory of Gp‐EVtPD1 in the recipient cells, tPD1 on the EV binds PDL1 and triggers the PDL1 endocytosis and degradation in endosomes/lysosomes in a sequential manner, and thus boosts the anti‐tumor immunity of cytotoxic T cells. In summary, Gp‐EVtPD1 serves as a novel ultrasound contrast agent and blocker of PDL1, which might be of great advantage in imaging PDL1 expression and conquering immune checkpoint blocker resistance. [ABSTRACT FROM AUTHOR]

Background. Despite the growing prevalence of internet usage among young people, the relationships between internet addiction, online social anxiety, fear of missing out (FoMO), and interpersonal sensitivity remain uncertain, intricate, and multifaceted. To gain insight into the underlying psychological mechanisms, we employed network analysis to explore the interconnections between them. This endeavor may provide fresh opportunities for intervention and treatment. Methods. In this study, 470 participants were assessed at age from 18 to 22 (M = 20 18 years, SD = 1 861) years. Network analysis was used to examine the connections between symptoms, and statistical measures were applied to assess the stability of the network model. Results. Online social anxiety and interpersonal sensitivity had the strongest associations with other symptoms in the network, with “Evaluation anxiety” having the highest expected influence centrality, followed by “Privacy concern anxiety,” “Need for approval,” “Suspicion,” and “vulnerability.” The FoMO symptom, “Fear of missing information,” had the strongest direct relation to internet addiction. “Evaluation anxiety” and “Fear of missing information” played a key role in bridging internet addiction and interpersonal sensitivity. Additionally, the structure distribution of edge weights had a significant difference between gender. Conclusions. Our findings indicated that FoMO, interpersonal sensitivity, and online social anxiety likely play a significant role in the development and continuation of internet addiction. Interpersonal sensitivity seems to contribute to increased online social anxiety, FoMO, and the development of internet addiction, indicating that targeting these symptoms may help reduce negative online behavior and psychological burden. [ABSTRACT FROM AUTHOR]

Conventional inorganic coagulants (Al, Fe) and Al/Fe-based covalently bonded flocculants (CAFMs) had different hydrolysis species at different pHs, which subsequently led to differences in their binding sites and complexation ability with humic acid (HA). Studying the binding sites and interactions between CAFMs, AlCl3 (Al), and FeCl3 (Fe) hydrolysates and HA molecules is critical to understanding the coagulation mechanism. The results found that CAFM 0.6, Al, and AlCl3 combined FeCl3 (Al/Fe) removed more than 90% of HA at pH 6, and CAFMs showed higher HA removal rate than that of Al, Fe, and Al/Fe under the same reaction conditions. The flocs of CAFMs contained abundant -NH2/OH as well as the large particle size, compact structure, and excellent settling performance. The hydrolyzed species of Al and Fe were predominantly Alb and Feb at pH 6, but the hydrolyzed species of CAFMs were primarily (Al + Fe)c. Moreover, the hydrolyzed species of Al and Al/Fe were found to complex with HA functional groups such as -COOH, C = O, C-H/C–C, C = C, and C–OH to form ligand bonds, while the hydrolyzed species (Al + Fe)c of CAFMs could deeply interact with HA functional groups including C-O, -COOH, C = O, C-H/C–C, C = C, and C–OH by the adsorption and sweeping. [ABSTRACT FROM AUTHOR]

Background: Osteoarthritis (OA) is a degenerative disease characterized by chronic inflammation of the joint. As the disease progresses, patients will gradually develop symptoms such as pain, physical limitations and even disability. The risk factors for OA include genetics, gender, trauma, obesity, and age. Unfortunately, due to limited understanding of its pathological mechanism, there are currently no effective drugs or treatments to suspend the progression of osteoarthritis. In recent years, some studies found that low-intensity pulsed ultrasound (LIPUS) may have a positive effect on osteoarthritis. Nonetheless, the exact mechanism by which LIPUS affects osteoarthritis remains unknown. It is valuable to explore the specific mechanism of LIPUS in the treatment of OA. Methods: In this study, we validated the potential therapeutic effect of LIPUS on osteoarthritis by regulating the YAP–RIPK1–NF-κB axis at both cellular and animal levels. To verify the effect of YAP on OA, the expression of YAP was knocked down or overexpressed by siRNA and plasmid in chondrocytes and adeno-associated virus was injected into the knee joint of rats. The effect of LIPUS was investigated in inflammation chondrocytes induced by IL-1β and in the post-traumatic OA model. Results: In this study, we observed that YAP plays an important role in the development of osteoarthritis and knocking down of YAP significantly inhibited the inflammation and alleviated cartilage degeneration. We also demonstrated that the expression of YAP was increased in osteoarthritis chondrocytes and YAP could interact with RIPK1, thereby regulating the NF-κB signal pathway and influencing inflammation. Moreover, we also discovered that LIPUS decreased the expression of YAP by restoring the impaired autophagy capacity and inhibiting the binding between YAP and RIPK1, thereby delaying the progression of osteoarthritis. Animal experiment showed that LIPUS could inhibit cartilage degeneration and alleviate the progression of OA. Conclusions: These results showed that LIPUS is effective in inhibiting inflammation and cartilage degeneration and alleviate the progression of OA. As a result, our results provide new insight of mechanism by which LIPUS delays the development of osteoarthritis, offering a novel therapeutic regimen for osteoarthritis. [ABSTRACT FROM AUTHOR]

A Spaceborne broadband circularly polarized conformal antenna for ultra high frequency band is presented. The antenna mounted on the outer surface of the circular arc spherical cone satellite works in 390–450 MHz frequency band, and has the characteristics of broadband, light miniature. The measured results for the antenna demonstrate an impedance bandwidth of 32.5% from 360 to 500 MHz, an axial ratio bandwidth of 27.2% from 380 to 500 MHz, and a peak gain of 6.7 dBic. The antenna form can be applied to the field of broadband circularly polarized conformal antenna on satellite and missile, and has a wide application prospect. [ABSTRACT FROM AUTHOR]

The vibration-based identification method has long been used as an alternative to mechanical tests for material characterization. Its non-destructive nature, high efficiency in parameter identification of anisotropic materials and its ability to achieve structure-level homogenization make it very attractive for both academic community and industry. Due to the absence of explicit mapping from the result of vibration test to elastic constants, conventional vibration-based identification approaches generally rely on the minimization of a cost function built on modal properties or Frequency Response Function (FRF) curve. In this paper, a direct relation from FRFs to mechanical properties is established by deep neural network, which offers a new perspective to solve the inverse identification problem. Specifically, this mapping is approximately built with Multilayer Perceptrons (MLPs) and the network's weights are learned from massive training data. The training data-i.e., the FRF curves, is generated by a finite element model whose input parameters are selected by Latin Hypercube Sampling (LHS). After training, the obtained model is validated by dataset unseen during training process and will be further used to predict mechanical properties of materials from FRFs obtained by vibration test. The effectiveness and efficiency of the developed method are demonstrated on the case study of an aluminum plate. Both the Young's modulus and Poisson's ratio are retrieved with high accuracy. [ABSTRACT FROM AUTHOR]

China's Shanxi Province accounts for 12 % of global coal output and therefore is responsible for a very large fraction of the total global methane (CH 4) emissions, as well as being a large source of uncertainty due to the lack of in situ and field measurements. This work introduces the first comprehensive attempt to compute the coal mine methane (CMM) emissions throughout Shanxi, using a mixture of bottom-up and top-down approaches. First, public and private data from 636 individual coal mines in Shanxi Province were analyzed following the IPCC Tier 2 approach, using three to five sets of observed emission factors and rank information based on methods issued by the National Coal Mine Safety Administration and the National Energy Administration, to compile a range of bottom-up CMM on a mine-by-mine basis. An eddy covariance tower is set up near the output flue of a well-characterized high-rank coal mine in Changzhi and used to produce an average observed CH 4 flux over two 2-month-long periods (Winter 2021 and Autumn 2022). The observed half-hourly CH 4 flux variability is found to be roughly stable over the entire observed time and is subsequently used to produce a set of scaling factors (ratio correction) to update the preliminary bottom-up coal mine methane emissions to account for both bias and high-frequency temporal variability. The resulting emissions dataset has been compared against commonly used global CMM datasets including EDGAR and GFEI v2, and there are three unique scientific conclusions. First, their total CH 4 emissions over Shanxi lie between this work's 50th percentile and 70th percentile range, meaning they are slightly high. Second, both datasets have a very large amount of emissions which occur where there are no coal mines and no CH 4 -emitting industry, indicating that there are significant spatial disparities, with the overlapped portion of CMM emissions where mines exist consistently close to the 30th percentile of this work's emissions, meaning they underestimate CMM in general on a mine-by-mine basis. Third, some of the mines have average emissions values which are more than the 90th percentile of the computed mine-by-mine emissions, while many are far below the 10th percentile, showing that there is a significant issue with the sampling not capturing the observed temporal variability. It is hoped that this mine-by-mine and high-frequency approximation of CMM emissions can both improve top-down observation campaigns and provide quantitative support and identification of mitigation opportunities. [ABSTRACT FROM AUTHOR]

Dipterocarpoideae species form the emergent layer of Asian rainforests. They are the indicator species for Asian rainforest distribution, but they are severely threatened. Here, to understand their adaptation and population decline, we assemble high-quality genomes of seven Dipterocarpoideae species including two autotetraploid species. We estimate the divergence time between Dipterocarpoideae and Malvaceae and within Dipterocarpoideae to be 108.2 (97.8‒118.2) and 88.4 (77.7‒102.9) million years ago, and we identify a whole genome duplication event preceding dipterocarp lineage diversification. We find several genes that showed a signature of selection, likely associated with the adaptation to Asian rainforests. By resequencing of two endangered species, we detect an expansion of effective population size after the last glacial period and a recent sharp decline coinciding with the history of local human activities. Our findings contribute to understanding the diversification and adaptation of dipterocarps and highlight anthropogenic disturbances as a major factor in their endangered status. Dipterocarp trees are iconic but severely threatened species in Asian rainforests. This study assembles high-quality genomes of seven dipterocarp species to reveal the molecular basis of key adaptations and identifies a recent sharp population decline coinciding with local human activity. [ABSTRACT FROM AUTHOR]

Dipterocarpoideae species form the emergent layer of Asian rainforests. They are the indicator species for Asian rainforest distribution, but they are severely threatened. Here, to understand their adaptation and population decline, we assemble high-quality genomes of seven Dipterocarpoideae species including two autotetraploid species. We estimate the divergence time between Dipterocarpoideae and Malvaceae and within Dipterocarpoideae to be 108.2 (97.8‒118.2) and 88.4 (77.7‒102.9) million years ago, and we identify a whole genome duplication event preceding dipterocarp lineage diversification. We find several genes that showed a signature of selection, likely associated with the adaptation to Asian rainforests. By resequencing of two endangered species, we detect an expansion of effective population size after the last glacial period and a recent sharp decline coinciding with the history of local human activities. Our findings contribute to understanding the diversification and adaptation of dipterocarps and highlight anthropogenic disturbances as a major factor in their endangered status. Dipterocarp trees are iconic but severely threatened species in Asian rainforests. This study assembles high-quality genomes of seven dipterocarp species to reveal the molecular basis of key adaptations and identifies a recent sharp population decline coinciding with local human activity. [ABSTRACT FROM AUTHOR]

Dynamic force reconstruction theory has been developed for many years, generating numerous effective methods. However, the applications of engineering structures are relatively rare, owing to the difficult experiment, low accuracy and noise disturbance, among others. Aiming at the support platform structure commonly used in engineering, we propose the complete dynamic force reconstruction process based on the direct inversion method in frequency domain. The dynamic calibration methods of experiment and simulation are both analyzed in this case. In order to improve identification accuracy, the Tikhonov regularization is introduced. The results validate the reliability of our proposed method in real scenarios and the necessity of regularization method. Moreover, dynamic load identification method of structural variable stiffness is derived by the four-terminal parameter method. This work provides engineering application–a reference for the engineering of dynamic load identification technology. [ABSTRACT FROM AUTHOR]

Grover quantum algorithm is an unstructured search algorithm that can run on a quantum computer with the complexity of O N , and is one of the typical algorithms of quantum computing. Recently, it has served as a routine for pattern-matching tasks. However, the original Grover search algorithm is probabilistic, which is not negligible for problems involving determinism. Besides that, efficient data loading is also a key challenge for the practical applications of the Grover algorithm. Here in this work, we propose a modified pattern-matching scheme with Long's quantum search algorithm, in which the quantum circuit structure search algorithm requires fewer multi-qubit quantum gates, and can obtain the desired results deterministically. Then, the comparison of the performance of our scheme and the previous algorithms is presented through numerical simulations, indicating our algorithm is feasible with current quantum technologies which is friendly to noisy intermediate-scale quantum (NISQ) devices. [ABSTRACT FROM AUTHOR]

Ochratoxin A (OTA) is one of the mycotoxins that poses a serious threat to human and animal health. Curcumin (CUR) is a major bioactive component of turmeric that provides multiple health benefits. CUR can reduce the toxicities induced by mycotoxins, but the underlying molecular mechanisms remain largely unknown. To explore the effects of CUR on OTA toxicity and identify the key regulators and metabolites involved in the biological processes, we performed metabolomic and transcriptomic analyses of livers from OTA-exposed mice. We found that CUR can alleviate the toxic effects of OTA on body growth and liver functions. In addition, CUR supplementation significantly affects the expressions of 1584 genes and 97 metabolites. Integrated analyses of transcriptomic and metabolomic data showed that the pathways including Arachidonic acid metabolism, Purine metabolism, and Cholesterol metabolism were significantly enriched. Pantothenic acid (PA) was identified as a key metabolite, the exogenous supplementation of which was observed to significantly alleviate the OTA-induced accumulation of reactive oxygen species and cell apoptosis. Further mechanistical analyses revealed that PA can downregulate the expression level of proapoptotic protein BAX, enhance the expression level of apoptosis inhibitory protein BCL2, and decrease the level of phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2). This study demonstrated that CUR can alleviate the adverse effects of OTA by influencing the transcriptomic and metabolomic profiles of livers, which may contribute to the application of CUR in food and feed products for the prevention of OTA toxicity. [ABSTRACT FROM AUTHOR]

In this paper, an X‐band dual‐band dual‐polarized medium‐gain antenna (MGA) is designed for deep‐space satellite applications. The MGA has isoflux radiation patterns in the 7.145–7.19 and 8.40–8.45 GHz frequency bands. The concentric ring horn qualification model named MGA is developed for TIAN WEN‐2 (TW‐2) mission. MGA is fabricated and the measurements are conducted on it and its installation on the deck of the TW‐2 Radiation Mockup (RM). The performance evaluation of MGA and its corresponding RM measurements indicate an axial ratio lower than 4.0 dB and a gain higher than 5.8 dBic on the operational bandwidth at the coverage limit of 40° for all azimuth angles (φ). The calculated and measured data align exceedingly well, confirming that MGA satisfactorily meets the requirements for the forthcoming TW‐2 deep‐space spacecraft. [ABSTRACT FROM AUTHOR]

In this report, a Ka‐band, broadband, omnidirectional, with a top metal disk antenna is proposed. The antenna is fed by an air waveguide. The TE10 mode of the waveguide can efficiently be converted into an air coaxial TEM mode by a compact waveguide air coaxial transition. After matching and adjusting, the dielectric substrate is loaded and then transited to the metal radiation structure loaded with a top metal disc to obtain omnidirectional electromagnetic waves efficiently converted to free space. By optimizing the sizes of waveguide to air‐coaxial transition structure and disk, and so on, a good broadband omnidirectional antenna performance is finally achieved. The measured fractional impedance bandwidth is larger than 30% with the reference of S11⩽−10 $\leqslant -10$ dB, which fully verifies the rationality of the design. In addition, measured patterns at the plane of different azimuth angles almost coincide with each other, which shows an excellent omnidirectional pattern. [ABSTRACT FROM AUTHOR]

While there is lively debate on whether higher education cultivates support for immigrants in North America and Western Europe, there is little discussion on the extent to which the relationship generalizes beyond these continents. In light of Japan's growing reliance on foreign workers, increase in university enrollment rates, as well as efforts to internationalize universities over the last half-century, we explore the relationship between university education and Japanese attitudes toward immigrants. Using two surveys asking an overlapping set of questions in 2009 and 2022, we find the relationship between university education and pro-immigrant attitudes varies by generation. Otherwise positive connections are significantly weakened for Japanese who entered universities in the 1990s through 2000s. Even though Japan is a modern democracy with well-developed higher education institutions, these institutions do not always correlate with more supportive attitudes toward immigrants. Our findings underscore the dynamic nature of higher education's role in shaping public opinion outside of North America and Western Europe. [ABSTRACT FROM AUTHOR]

Natural montane peatlands are generally not a significant source of nitrous oxide (N2O) due to environment limitations, including phosphorus (P) scarcity and temperature lowness. Phosphorus enrichment and warming caused by global change are altering these limitations, and are likely to increase the source function of N2O. However, the combined effects of P addition and warming on N2O fluxes and biotic/abiotic factors in peatlands are still uncertain. To address this, we investigated the long-term (12 yrs) effects of P addition (5 and 10 kg ha−1 yr−1) and its interaction with warming on N2O fluxes in a peatland. The results showed that although long-term P addition did not significantly affect the source/sink function of N2O in the peatland, it stimulated enzyme activities and promoted peat decomposition. However, warming amplified the effect of P addition to increase N2O emissions by stimulating enzyme activities and changing soil stoichiometry, so even turned the peatland into a significant source of N2O with an emission of approximate 100 g m−2 during the growing season. Our study suggests that P enrichment against the current background of global warming will enhance the possibility of strong N2O emissions in montane peatlands, which may increase the risk that global warming will be further aggravated. [ABSTRACT FROM AUTHOR]

River water surface extent can be extracted from optical and radar satellite images; this is useful for estimating streamflow from space. The radiation characteristics of open water from the visible and microwave bands are different and provide independent information. In this study, for the purpose of improving streamflow estimation from space for data-sparse regions, a method that combines satellite optical and radar images data for streamflow estimation using a machine learning technique was proposed. The method was demonstratedthrough a case study in the river segment upstream of the Ganzi gauging station on the Yalong River, China. Utilizing the support vector regression (SVR) model, the feasibility of different combinations of water surface area derived from Sentinel-1 synthetic aperture radar images (AREA_SAR), modified normalized difference water index derived from Landsat 8 images (MNDWI), and reflectance ratios between NIR and SWIR channels derived from MODIS images (RNIR/RSWIR) for streamflow estimation were evaluated through three experiments. In Experiment I, three models using AREA_SAR (Model 1), MNDWI (Model 2), and a combination of AREA_SAR and MNDWI (Model 3) were built; the mean relative error (MRE) and mean absolute error (MAE) of streamflow estimates corresponding to the SVR model using both AREA_SAR and MNDWI (Model 3) were 0.19 and 31.6 m3/s for the testing dataset, respectively, and were lower than two models using AREA_SAR (Model 1) or MNDWI (Model 2) solely as inputs. In Experiment II, three models with AREA_SAR (Model 4), RNIR/RSWIR (Model 5), and a combination of AREA_SAR and RNIR/RSWIR (Model 6) as inputs were developed; the MRE and MAE for the model using AREA_SAR and RNIR/RSWIR (Model 6) were 0.25 and 56.5 m3/s, respectively, which outperformed the two models treating AREA_SAR (Model 4) or MNDWI (Model 5) as single types of inputs. In Experiment III, three models using AREA_SAR (Model 7), MNDWI, and RNIR/RSWIR (Model 8) and the combination of AREA_SAR, MNDWI and RNIR/RSWIR (Model 9) were built; combining all three types of satellite observations (Model 9) exhibited the highest accuracy, for which the MRE and MAE were 0.18 and 18.4 m3/s, respectively. The results of all three experiments demonstrated that integrating optical and microwave observations could improve the accuracy of streamflow estimates using a data-driven model; the proposed method has great potential for near-real-time estimations of flood magnitude or to reconstruct past variations in streamflow using historical satellite images in data-sparse regions. [ABSTRACT FROM AUTHOR]

China stands out as a major contributor to anthropogenic methane (CH4) emissions, with coal mine methane (CMM) playing a crucial role. To control and reduce CH4 emissions, China has made a dedicated commitment and formulated an ambitious mitigation plan. To verify the process made, the consistent acquisition of independent CH4 emission data is required. This paper aims to implement a wind-assigned anomaly method for the precise determination of regional-scale CMM emissions within the coal-rich Shanxi province. We use the TROPOspheric Monitoring Instrument (TROPOMI) CH4 observations from May 2018 to May 2023, coupled with ERA5 wind covering the Changzhi, Jincheng and Yangquan regions. The derived emission strengths are 8.4× 1026 ± 1.6 × 1025 molec. s-1 (0.706 ± 0.013 Tg yr-1), 1.4 × 1027 ± 1.9 × 1025 molec. s-1 (1.176 ± 0.016 Tg yr-1), and 4.9 × 1026± 1.8 × 1025 molec. s-1 (0.412 ± 0.015 Tg yr-1), respectively. Our results exhibit biases of -18 %, 8 %, and 14 % when compared to the bottom-up inventory. Larger discrepancies are found when comparing the estimates to the CAMS-GLOB-ANT and EDGARv7.0 inventories. This suggests that the two inventories may be overestimating the CH4 emissions in the Jincheng and Yangquan regions. Our estimates provide a comprehensive characterization of the regions within the Shanxi province, contribute to the validation of emission inventories, and help to develop climate mitigation strategies. [ABSTRACT FROM AUTHOR]

Introduction: Plants that display heteroblasty possess conspicuous variations in leaf morphology between their juvenile and adult phases, with certain species retaining juvenile-like leaves even in adulthood. Nevertheless, the ecological advantages of maintaining two or more distinct leaf types in heteroblastic plants at the adult stage remain unclear. Method: The aim of this study is to examine the adaptive significance of heteroblastic leaves sampled from branches with divergent functions (sterile and fertile branches) of mature Ficus pumila individuals by comparing their morphological, anatomical, and physiological characteristics. Result: Leaves on sterile branches (LSs) exhibited a significantly larger specific leaf area, thinner palisade and spongy tissues, lower chlorophyll contents, and lower light saturation points than leaves on fertile branches (LFs). These results demonstrate that LSs are better adapted to low light environments, while LFs are well equipped to take advantages of high light conditions. However, both LFs and LSs have a low light compensation point with no significant difference between them, indicating that they start to accumulate photosynthetic products under similar light conditions. Interestingly, significant higher net photosynthetic rate was detected in LFs, showing they have higher photosynthetic capacity. Furthermore, LFs produced significant more nutrients compared to LSs, which may associate to their ability of accumulating more photosynthetic products under full light conditions and higher photosynthetic capacity. Discussion: Overall, we observed a pattern of divergence in morphological features of leaves on two functional branches. Anatomical and physiological features indicate that LFs have an advantage in varied light conditions, providing amounts of photosynthetic products to support the sexual reproduction, while LSs adapt to low light environments. Our findings provide evidence that heteroblasty facilitates F. pumila to utilize varying light environments, likely associated with its growth form as a climbing plant. This strategy allows the plant to allocate resources more effectively and optimize its overall fitness. [ABSTRACT FROM AUTHOR]

In recent years, an increasing number of studies have examined the mechanisms underlying nonverbal emotional information processing in people with high social anxiety (HSA). However, most of these studies have focused on the processing of facial expressions, and there has been scarce research on gesture or even face-gesture combined processing in HSA individuals. The present study explored the processing characteristics and mechanism of the interaction between gestures and facial expressions in people with HSA and low social anxiety (LSA). The present study recruited university students as participants and used the Liebowitz Social Anxiety Scale scores to distinguish the HSA and LSA groups. We used a 2 (group: HSA and LSA) x 2 (emotion valence: positive, negative) x 2 (task: face, gesture) multifactor mixed design, and videos of a single face or gesture and combined face-gesture cues were used as stimuli. We found that (1) there is a distinction in the processing of faces and gestures, with individuals recognizing gestures faster than faces; (2) there is an attentional enhancement in the processing of gestures, particularly for negative gestures; and (3) when the emotional valence of faces and gestures align, it facilitates the recognition of both. However, incongruent gestures have a stronger impact on the processing of facial expressions compared to facial expressions themselves, suggesting that the processing of facial emotions is more influenced by environmental cues provided by gestures. These findings indicated that gestures played an important role in emotional processing, and facial emotional processing was more dependent on the environmental cues derived from gestures, which helps to clarify the reasons for biases in the interpretation of emotional information in people with HSA. [ABSTRACT FROM AUTHOR]

Purpose: To biomechanically compare the initial fixation strength of grafts among three tibial tunnel angles (30°/45°/60°) in transtibial posterior cruciate ligament (PCL) reconstruction. Methods: A series of transtibial PCL reconstruction models were established with porcine tibias and bovine tendons. Specimens were randomly assigned to three groups according to the angles between the tibial tunnel and the perpendicular line of the tibial shaft: Group A (30°, n = 12), Group B (45°, n = 12), and Group C (60°, n = 12). The area of the tunnel entrance, the segmental bone mineral density (sBMD) of the graft fixation site of the tibia and the maximum insertion torque of the interference screw were measured. Finally, load to failure tests were carried out on the graft-screw-tibia constructs at the same rate. Results: Ultimate load to failure in Group C (335.2 ± 107.5 N) was significantly lower than that in Group A (584.1 ± 127.9 N, P < 0.01) and Group B (521.9 ± 95.9 N, P < 0.01). There were no significant differences between biomechanical properties of Groups A and B (n.s.). The posterior part fractures of the tibial tunnel exit occurred in eight specimens of Group C. In addition, the ultimate load was proven to be related to insertion torque (rho = 0.7, P < 0.01), sBMD (rho = 0.7, P < 0.01), and the area of the tunnel entrance (rho =− 0.4, P = 0.01). Conclusion: The ultimate load to failure was significantly lower in tibial PCL interference screw fixation for tunnels drilled at 60° compared to 30°/45°. In addition, the ultimate load was significantly correlated with insertion torque, sBMD and the area of the tunnel entrance. Given that the load to failure of distal fixation may not be sufficient for early postoperative rehabilitation, a 60° tunnel should not be recommended to drill in tibia during PCL reconstruction. [ABSTRACT FROM AUTHOR]

In this work, silicone polymer (PSOL)/carbon fiber (CF)/epoxy (EP) composites were prepared by introducing PSOL as interfacial modifiers into CF/EP composites. The mechanical and dynamic mechanical properties of the composites were investigated, and the optimum content of the PSOL modification was obtained. The impact of EP on the wettability of CF was compared before and after adding PSOL. The micromorphology of CF/EP composites was also observed. The consequences demonstrate that the interfacial bonding ability between CF and matrix was improved after adding PSOL. The interlaminar shear strength, tensile strength, elongation at break and flexural strength of PSOL/CF/EP composites were increased by 23.28%, 18.49%, 38.45% and 11.23%, respectively. Furthermore, the original thermal stability of the composite was preserved after the modification of PSOL. [ABSTRACT FROM AUTHOR]

China's Shanxi Province accounts for 12 % of global coal output, and therefore is responsible for a very large fraction of the total global methane (CH4) emissions, as well as being a large source of uncertainty due to the lack of in-situ and field measurements. This work introduces the first comprehensive attempt to compute the coal mine methane emissions (CMM) throughout Shanxi, using a mixture of bottom-up and top-down approaches. First, public and private data from 636 individual coal mines in Shanxi Province were analyzed following the IPCC Tier 2 approach, using three to five sets of observed emission factors, and rank information based on methods issued by the National Coal Mine Safety Administration and the National Energy Administration, to compile a range of bottom-up CMM on a mine-by-mine basis. An eddy-covariance tower is set up near the output flue of a well-characterized high rank coal mine in Changzhi, and used to produce an average observed CH4 flux over two two-month long periods (Winter 2021 and Autumn 2022). The observed half-hourly CH4 flux variability is found to be roughly stable over the entire observed time, and is subsequently used to produce a set of scaling factors (RATIO correction) to updating the preliminary bottom-up coal mine methane emissions to account for both bias and high-frequency temporal variabiliy. The resulting emissions dataset have been compared against commonly used global CMM datasets including EDGAR and GFEI v2, and yield three unique scientific conclusions. First, their total CH4 emissions over Shanxi lie in between this work's 50th percentile and 70th percentile range, meaning they are slightly high. Second, both datasets have a very large amount of emissions which occur where there are no coal mines and no CH4 emitting industry, indicating that there are significant spatial disparities, with the overlapped portion of CMM emissions where mines exist consistently close to the 30th percentile of this work's emissions, meaning they underestimate CMM in general on a mine-by-mine basis. Third, some of the mines have average emissions values which are more than the 90th percentile of the computed mine-by-mine emissions, while many are far below the 10th percentile, showing that there is a significant issue with the sampling not capturing the observed temporal variability. It is hoped that this mine-by-mine and high frequency approximation of CMM emissions can improve both top-down observation campaigns as well as provide quantitative support and identification of mitigation opportunities. [ABSTRACT FROM AUTHOR]

Nano zero-valent iron (nZVI) has a great potential for arsenic removal, but it would form aggregates easily and consume largely by H+ in the strongly acidic solution. In this work, 15%CaO doped with nZVI (15%CaO-nZVI) was successfully synthesized from a simplified ball milling mixture combined with a hydrogen reduction method, which had a high adsorption capacity for As(V) removal from high-arsenic acid wastewater. More than 97% As(V) was removed by 15%CaO-nZVI under the optimum reaction conditions of pH 1.34, initial As(V) concentration 16.21 g/L, and molar ratio of Fe/As (nFe/nAs) 2.5:1. The effluent pH solution was weakly acidic 6.72, and the secondary arsenic removal treatment reduced the solid waste and improved arsenic grade in slag from the mass fraction of 20.02% to 29.07%. Multiple mechanisms including Ca2+ enhanced effect, adsorption, reduction, and co-precipitation coexisted for As(V) removal from high-arsenic acid wastewater. Doping of CaO might lead to improving cracking channels which was benefit for electronic transmission and the confusion of atomic distribution. The in situ weak alkaline environment generated on the surface of 15%CaO-nZVI would increase the content of γ-Fe2O3/Fe3O4, which was in favor for As(V) adsorption. In addition, H+ in the strongly acidic solution could accelerate corrosion of 15%CaO-nZVI and abundant fresh and reactive iron oxides continuously generated, which would provide plenty specific reactive site and fast charge transfer and ionic mobility for arsenic removal. [ABSTRACT FROM AUTHOR]

Tidal-level prediction is crucial for ensuring the safety and efficiency of offshore marine activities, port and channel management, water transportation resource development, and life-saving operations. Although tidal harmonic analysis is among the most prevalent methods for predicting tidal water level fluctuations, it relies on extensive data, and its long-term prediction accuracy can be limited. To enhance prediction performance, this paper proposes a model that combines the variational mode decomposition (VMD) algorithm with the long short-term memory (LSTM) neural network. The initial step involves decomposing the original data using the VMD algorithm, followed by applying the LSTM to each decomposition component. Finally, all prediction results are superimposed and summed. The model is tested using the 2018 tidal time series data from the Lvsi station in Zhoushan City and the 2020 tidal time series data from the Ganpu station. The results are compared with those from the classical harmonic analysis model, the traditional machine learning model, and the decomposition-based machine learning method. The experimental outcomes demonstrate the superior predictive capabilities of the proposed model. [ABSTRACT FROM AUTHOR]

RhoGDIα is an inhibitor of RhoGDP dissociation that involves in Aβ metabolism and NFTs production in Alzheimer's disease (AD) by regulating of RhoGTP enzyme activity. Our previous research revealed that RhoGDIα, as the target of Polygala saponin (Sen), might alleviate apoptosis of the nerve cells caused by hypoxia/reoxygenation (H/R). To further clarify the role of RhoGDIα in the generation of NFTs, we explored the relationship between RhoGDIα and Tau. We found out that RhoGDIα and Tau can bind with each other and interact by using coimmunoprecipitation (Co-IP) and GST pulldown methods in vitro. This RhoGDIα-Tau partnership was further verified by using immunofluorescence colocalization and fluorescence resonance energy transfer (FRET) approaches in PC12 cells. Using the RNA interference (RNAi) technique, we found that the RhoGDIα may be involved in an upstream signaling pathway for Tau. Subsequently, in Aβ25-35- and H/R-induced PC12 cells, forced expression of RhoGDIα via cDNA plasmid transfection was found to reduce the hyperphosphorylation of Tau, augment the expression of bcl-2 protein, and inhibit the expression of Bax protein (reducing the Bax/bcl-2 ratio) and the activity of caspase-3. In mouse AD and VaD models, forced expression of RhoGDIα via injection of a viral vector (pAAV-EGFP-RhoGDIα) into the lateral ventricle of the brain alleviated the pathological symptoms of AD and VaD. Finally, GST pulldown confirmed that the binding sites on RhoGDIα for Tau were located in the range of the ΔC33 fragment (aa 1–33). These results indicate that RhoGDIα is involved in the phosphorylation of Tau and apoptosis in AD and VaD. Overexpression of RhoGDIα can inhibit the generation of NFTs and delay the progress of these two types of dementia. [ABSTRACT FROM AUTHOR]

Usher syndrome (USH) is characterised by degenerative vision loss known as retinitis pigmentosa (RP), sensorineural hearing loss, and vestibular dysfunction. RP can cause degeneration and the loss of rod and cone photoreceptors, leading to structural and functional changes in the retina. Cep250 is a candidate gene for atypical Usher syndrome, and this study describes the development of a Cep250 KO mouse model to investigate its pathogenesis. OCT and ERG were applied in Cep250 and WT mice at P90 and P180 to access the general structure and function of the retina. After recording the ERG responses and OCT images at P90 and P180, the cone and rod photoreceptors were visualised using an immunofluorescent stain. TUNEL assays were applied to observe the apoptosis in Cep250 and WT mice retinas. The total RNA was extracted from the retinas and executed for RNA sequencing at P90. Compared with WT mice, the thickness of the ONL, IS/OS, and whole retina of Cep250 mice was significantly reduced. The a-wave and b-wave amplitude of Cep250 mice in scotopic and photopic ERG were lower, especially the a-wave. According to the immunostaining and TUNEL stain results, the photoreceptors in the Cep250 retinas were also reduced. An RNA-seq analysis showed that 149 genes were upregulated and another 149 genes were downregulated in Cep250 KO retinas compared with WT mice retinas. A KEGG enrichment analysis indicated that cGMP-PKG signalling pathways, MAPK signalling pathways, edn2-fgf2 axis pathways, and thyroid hormone synthesis were upregulated, whereas protein processing in the endoplasmic reticulum was downregulated in Cep250 KO eyes. Cep250 KO mice experience a late-stage retinal degeneration that manifests as the atypical USH phenotype. The dysregulation of the cGMP-PKG-MAPK pathways may contribute to the pathogenesis of cilia-related retinal degeneration. [ABSTRACT FROM AUTHOR]