1. Phylogeographic analysis of porcine reproductive and respiratory syndrome virus 1 in Guangdong province, Southern China
- Author
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Zhang-Fu Pei, He Zhang, Shao-Lun Zhai, Xiao-Hui Wen, Wen-Kang Wei, Xia Zhou, Dian-Hong Lv, Qin-Ling Chen, Zuzhang Wei, and Tao Lin
- Subjects
0301 basic medicine ,China ,Veterinary medicine ,Farms ,Genotype ,Swine ,viruses ,animal diseases ,Porcine Reproductive and Respiratory Syndrome ,Sequence alignment ,Open Reading Frames ,Viral Proteins ,03 medical and health sciences ,Phylogenetics ,Virology ,Prevalence ,Animals ,Pig farming ,Porcine respiratory and reproductive syndrome virus ,Amino Acid Sequence ,Pathogen ,Phylogeny ,Sequence Homology, Amino Acid ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,virus diseases ,General Medicine ,respiratory system ,Porcine reproductive and respiratory syndrome virus ,biology.organism_classification ,Molecular Typing ,Phylogeography ,030104 developmental biology ,RNA, Viral ,Sequence Alignment - Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is considered an important economic pathogen for the international swine industry. At present, both PRRSV-1 and PRRSV-2 have been confirmed to be co-circulating in China. However, there is little available information about the prevalence or distribution of PRRSV-1 in Guangdong province, southern China. In this study, we performed molecular detection of PRRSV-1 in 750 samples collected from 50 farms in 15 major pig farming regions in this province. After RT-PCR testing, 64% (32/50) of farms were confirmed as PRRSV-1-positive. Surprisingly, PRRSV-1 was circulating on at least one pig farm in all 15 regions; of the 750 samples, 186 samples (24.8%) were positive for PRRSV-1. Furthermore, 15 representative PRRSV-1 ORF5 sequences (606 bp) (n = 1 per region) were obtained from those PRRSV-1-positive regions. Sequence alignment analysis indicated that they shared 81.8% ~ 100% nucleotide and 81.2% ~ 100% amino acid similarity with each other. Although all current PRRSV-1 sequences were divided into pandemic subtype 1, most of them had unique glycoprotein-5 amino acid sequences that are significantly different from other known PRRSV-1 isolates. To conclude, the present findings revealed wide geographical distribution of PRRSV-1 in Guangdong province, southern China. This study further extends the epidemiological significance of PRRSV-1 in China.
- Published
- 2018