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Phylogeographic analysis of porcine reproductive and respiratory syndrome virus 1 in Guangdong province, Southern China
- Source :
- Archives of Virology. 163:2443-2449
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Porcine reproductive and respiratory syndrome virus (PRRSV) is considered an important economic pathogen for the international swine industry. At present, both PRRSV-1 and PRRSV-2 have been confirmed to be co-circulating in China. However, there is little available information about the prevalence or distribution of PRRSV-1 in Guangdong province, southern China. In this study, we performed molecular detection of PRRSV-1 in 750 samples collected from 50 farms in 15 major pig farming regions in this province. After RT-PCR testing, 64% (32/50) of farms were confirmed as PRRSV-1-positive. Surprisingly, PRRSV-1 was circulating on at least one pig farm in all 15 regions; of the 750 samples, 186 samples (24.8%) were positive for PRRSV-1. Furthermore, 15 representative PRRSV-1 ORF5 sequences (606 bp) (n = 1 per region) were obtained from those PRRSV-1-positive regions. Sequence alignment analysis indicated that they shared 81.8% ~ 100% nucleotide and 81.2% ~ 100% amino acid similarity with each other. Although all current PRRSV-1 sequences were divided into pandemic subtype 1, most of them had unique glycoprotein-5 amino acid sequences that are significantly different from other known PRRSV-1 isolates. To conclude, the present findings revealed wide geographical distribution of PRRSV-1 in Guangdong province, southern China. This study further extends the epidemiological significance of PRRSV-1 in China.
- Subjects :
- 0301 basic medicine
China
Veterinary medicine
Farms
Genotype
Swine
viruses
animal diseases
Porcine Reproductive and Respiratory Syndrome
Sequence alignment
Open Reading Frames
Viral Proteins
03 medical and health sciences
Phylogenetics
Virology
Prevalence
Animals
Pig farming
Porcine respiratory and reproductive syndrome virus
Amino Acid Sequence
Pathogen
Phylogeny
Sequence Homology, Amino Acid
biology
Reverse Transcriptase Polymerase Chain Reaction
virus diseases
General Medicine
respiratory system
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
Molecular Typing
Phylogeography
030104 developmental biology
RNA, Viral
Sequence Alignment
Subjects
Details
- ISSN :
- 14328798 and 03048608
- Volume :
- 163
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....505deee467a4c7ffa5e75c543dbbb17c