1. CD63
- Author
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Yohei, Otsuki, Eiko, Ito, Atsushi, Mukai, Morio, Ueno, Takahiro, Yamawaki, Chie, Sotozono, Shigeru, Kinoshita, and Junji, Hamuro
- Subjects
Male ,Tetraspanin 30 ,Tumor Necrosis Factor-alpha ,Macrophages ,Blotting, Western ,Enzyme-Linked Immunosorbent Assay ,Cell Communication ,Retinal Pigment Epithelium ,Exosomes ,Flow Cytometry ,Real-Time Polymerase Chain Reaction ,Coculture Techniques ,Immunity, Innate ,Cell Line ,Mice, Inbred C57BL ,Extracellular Vesicles ,Mice ,Gene Expression Regulation ,Animals ,Cytokines ,Chemokine CCL2 ,Signal Transduction - Abstract
The aim of the study is to clarify the participation of extracellular vesicles (EV) secreted by murine primary retinal pigment epithelial (mpRPE) cells in the cell to cell communication with macrophages (Mps), firstly described by the authors in 2016. In ocular inflammation, Mps act as sources of tumor necrosis factor-α (TNF-α), an activator of RPE cells. TNF-α stimulates the production of monocyte chemotactic protein (MCP-1) by RPE cells, thereby causing greater recruitment of Mps to the sub-RPE space. Murine RAW 264.7 Mps cells were co-cultured with C57BL/6 mouse mpRPE cells, either together or separated in transwells, vertically or horizontally connectable, with 0.40 or 0.03 μm membrane filters. The association of EV with mpRPE or RAW 264.7 was quantified by fluorescence cell sorting (FACS) using Qdot655 streptavidin-conjugated biotinylated EV. Increased levels of CD63
- Published
- 2020