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CD63
- Source :
- Experimental eye research. 205
- Publication Year :
- 2020
-
Abstract
- The aim of the study is to clarify the participation of extracellular vesicles (EV) secreted by murine primary retinal pigment epithelial (mpRPE) cells in the cell to cell communication with macrophages (Mps), firstly described by the authors in 2016. In ocular inflammation, Mps act as sources of tumor necrosis factor-α (TNF-α), an activator of RPE cells. TNF-α stimulates the production of monocyte chemotactic protein (MCP-1) by RPE cells, thereby causing greater recruitment of Mps to the sub-RPE space. Murine RAW 264.7 Mps cells were co-cultured with C57BL/6 mouse mpRPE cells, either together or separated in transwells, vertically or horizontally connectable, with 0.40 or 0.03 μm membrane filters. The association of EV with mpRPE or RAW 264.7 was quantified by fluorescence cell sorting (FACS) using Qdot655 streptavidin-conjugated biotinylated EV. Increased levels of CD63
- Subjects :
- Male
Tetraspanin 30
Tumor Necrosis Factor-alpha
Macrophages
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Cell Communication
Retinal Pigment Epithelium
Exosomes
Flow Cytometry
Real-Time Polymerase Chain Reaction
Coculture Techniques
Immunity, Innate
Cell Line
Mice, Inbred C57BL
Extracellular Vesicles
Mice
Gene Expression Regulation
Animals
Cytokines
Chemokine CCL2
Signal Transduction
Subjects
Details
- ISSN :
- 10960007
- Volume :
- 205
- Database :
- OpenAIRE
- Journal :
- Experimental eye research
- Accession number :
- edsair.pmid..........86469ef1697e3cee947f8e84f899ca6d