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Introduction: Our previous study demonstrated mild increase in serum concentration of anti- inflammatory cytokine IL-10 after comsuption of omega-3 polyunsaturated fatty acids enriched hen eggs in young healthy participants (Stupin et al., 2020). Therefore, the aim of this study was to determine serum concentrations of inflammatory and anti-inflammatory cytokines in young healthy individuals who consumed hen eggs enriched with four components - omega-3 polyunsaturated fatty acids, vitamin E, lutein and selenium. Methods: Randomized, double-blind placebo- controlled study was conducted in healthy young subjects (N=35) who consumed 3 chicken eggs per day for 3 weeks. Subjects in the "Quatro" group consumed enriched hen eggs, and control subjects consumed ordinary hen eggs, produced on the same farm. Serum concentration of IL- 17A, IL-10, IL-6 and TNF-alpha was determined by commercially available kit and analyzed on Luminex platform. One way ANOVA was conducted for statistical analysis with Tukey post hoc test ; p

Introduction: Previously we showed (Mihaljevic et al. 2018) that acute hyperbaric oxygenation (A-HBO2) transiently impaired vasorelaxation due to increased oxidative stress but also that intermittent (4d-HBO2) hyperbaric treatments again increased vasorelaxation of rat aorta and furthermore brought the antioxidative status to the normal level in the macrocirculation. The present study assessed the effects of different types of HBO2 treatments on gene expression of antioxidative enzymes in cerebral microcirculation (brain blood vessels (BBVs)). Methods: 9-11-weeks old healthy male Sprague-Dawley rats were randomly assigned to the following groups (n=6-8 rats/group): CONTROL group (intact animals), and 2 hyperbaric groups that received either single (A-HBO2) or intermitent (4 consecutive HBO2 treatments, one per day, 4d-HBO2) hyperbaric treatments. The therapy protocol consists of 120- minute sessions of 100% O2 at 2.0 bars absolute of pressure. Following the protocol, rats were anesthetized with ketamine (75 mg/kg) and midazolam (2.5 mg/kg) and sacrificed by decapitation. All surface BBVs were isolated and collected for mRNA levels measurements of antioxidative ezymes. Data were analyzed using One-Way ANOVA, present as mean±SD and p

Objective: Mediterranean lifestyle is slowly disappearing along the Croatian coast and Croatian islands were supposed to be the last part of healthy living. Unfortunately, healthcare system is poorer on islands than in other parts of Croatia. Our aim was to analyse lifestyle and hypertension (HT) treatment and control among inhabitants of the Croatian islands. Design and method: HSK was organised by the Croatian Society of Hypertension and conducted by the WG- medical students in hypertension. A screening site was set up at the boat by which students traveled from island to island. Adult volunteers were recruited through opportunistic screening on five Adriatic islands. Trained medical students collected information through an extended questionnaire. HT was defined as blood pressure (BP) > 140/90mmHg (single occasion ; an average of 2nd–3rd measurement ; Omron M3) and/or self-reported use of drugs for HT. Results were compared with results from the general Croatian population (EHUH 2 cohort). Results: A total of 424 participants were included (196 men, average age 61 years). There were less obese and current smokers at islands than in the general Croatian population (26.5% vs. 33.1% ; 21.5% vs. 25%, respectively, p < 0.05). Islanders were more physically active (> 1 time/week), eat more frequently fish (> 1 time/week), and use more frequently olive oil (daily basis) (44.1% vs. 26%, 79.9% vs. 32.5%, 72.6% vs. 14.9 %, respectively ; p < 0.01). However, there were no differences in consumption of red meat, processed meat, cookies and cakes (p > 0.05). Less hypertensive patients were treated and control was achieved in less subjects on islands than in the general population (60.7% vs. 72.9% ; 27.2% vs. 39.3%, respectively ; p < 0.01). Conclusions: Some elements of Mediterranean life are still present on Croatian islands. However, several poor lifestyle habits are equally present on islands and in other Croatian regions. Treatment and control of HT are poorer on islands reflecting inadequate healthcare and lack of physicians. Public health authorities should reorganize health care on islands. In the meantime, such public health actions are warranted and must continue.