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Objectives: Introduction Fractal analysis (FA) is a computational method used to quantify the complex trabecular structure of bone. While FA has been widely applied in dentistry, there are challenges in standardizing the technique due to factors such as image resolution, region of interest (ROI) selection, and image processing. This study aimed to investigate the impact of the direction of ROI selection (DROIS) on fractal dimension (FD) calculations., Methods: Panoramic radiographs of 226 individuals aged 20-35 years were analyzed. ROIs were selected on the mandibular condyle, angular region, and mental region, and oriented at 0°, 22.5°, 45°, and 67.5° angles. FD was calculated using the box-counting method in ImageJ. The Friedman test and Wilcoxon signed-rank test were used for statistical analysis., Results: The FD values differed significantly between the angled ROI groups in all three regions (Friedman test, p < 0.0001). Pairwise comparisons showed significant differences in FD between most ROI orientations, except between 22.5° and 67.5° in the angular region., Conclusions: DROIS is an important factor that should be considered in FA studies to ensure reliable and reproducible FD values. Appropriate methodological choices can help account for the influence of DROIS on FD calculations.., Competing Interests: Declarations Conflict of interest There is no conflict of interest among authors. Ethical approval All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2008. Informed consent was obtained from all patients for being included in the study., (© 2024. The Author(s) under exclusive licence to Japanese Society for Oral and Maxillofacial Radiology.)

Objectives: To assess the structural alterations in peri-implant bone occurring 5 years after prosthodontic loading in panoramic radiography (PR). Methods: PR images of 44 mandibular and 33 maxillary implants along with 42 healthy control teeth taken before and 5 years after prosthodontic loading were included. Two regions-of-interest (ROI) were selected from mesial and distal surrounding bone of each implant and tooth. Then, the selected ROIs were divided to obtain three sub-ROIs (coronal, middle, and apical) on each side. A total of eight ROIs and sub-ROIs from each implant and control tooth were used for the calculations of fractal dimension (FD), lacunarity, and bone area fraction (BA/TA). The paired-sample t test was used to compare measurements before and 5 years after loading (p = 0.05). Results: Overall evaluation of 77 implants showed that FD decreased at the middle and apical peri-implant bone levels 5 years after loading (p < 0.05). In mandibular implants, BA/TA decreased after loading (p < 0.05). While FD decreased at the coronal level (p = 0.022), lacunarity increased at the middle level of mandibular implants (p < 0.05). In maxillary implants, FD decreased at the middle and BA/TA decreased at the coronal level (p < 0.05). On the other hand, BA/TA increased at the apical level of maxillary implants (p = 0.016) after loading. None of the parameters revealed any difference in the control group (p > 0.05). Conclusions: FD and BA/TA can be used to analyze structural changes in peri-implant bone after prosthodontic loading. Additionally, FD, lacunarity and BA/TA may provide useful information about changes occurring at different levels of peri-implant bone. [ABSTRACT FROM AUTHOR]

Objective: The aims of this study were (1) to subjectively quantify the degree of scratching and smudging that had taken place in the junior clinic in the 9 months following the implementation of digital radiology; (2) to compare the findings with a previously published report; and (3) to identify areas in the protocol and training that can be refined to minimize future scratching and smudging., Methods: Seven sets of blank clinical photostimulable storage phosphor (PSP) plates were scanned after exposing them at 65 kV and 7 mA for 0.80 s. Scanned plates were lightly wiped with a soft cloth and alcohol, repackaged in plastic sleeves, re-exposed and rescanned. The two sets of resulting images were subjectively rated independently by two investigators for artefacts and placed in five categories., Results: Of all the images, approximately 75% were rated in the top 3 categories (most readable), leaving 17% and 8% in the poor and unsatisfactory categories, respectively. Mean rated values of the two image sets (before and after wiping) were not statistically different, but ratings slightly improved after cleaning the plates., Conclusions: Wiping all plates to remove surface contamination may not always be necessary or desirable. Systems that are designed to minimize handling of the plates may help minimize scratching of them.

Purpose: The aim of this retrospective study was to evaluate the effects of functional orthopedic treatment on mandibular bone structure using fractal dimension (FD) analysis of panoramic radiographs., Methods: The study material consisted of the pre- and posttreatment digital panoramic radiographs of 80 patients (mean age 10.1 ± 2.01 years; 45 girls, 35 boys) with class II malocclusion who were treated with the monoblock or twin block appliances. The following regions of interest (ROI) were selected: ROI1, condylar process; ROI2, angulus mandibulae; ROI3, corpus mandibulae; and ROI4, mental foramen. Pre- and posttreatment FD values were compared for each ROI., Results: FD values of the mandibular condyle did not change with the functional orthodontic treatment. FD values of the mandibular corpus region had the highest pretreatment values and significantly decreased with treatment (p < 0.05)., Conclusion: Functional orthopedic treatment altered the trabeculation of the mandibular bone, but it is speculated that the changes in the occlusal forces seemed to be of primary significance for this effect., (© 2022. Springer Medizin Verlag GmbH, ein Teil von Springer Nature.)

Objective: The objective of this study was to determine if phosphor plates used in predoctoral clinics are microbiologically contaminated and to identify the source of contamination., Study Design: Forty-five of 300 phosphor plates (15%) were randomly selected for examination. The plates were pressed into individual blood agar plates, were incubated using standard techniques at 37 degrees C, and were monitored for 72 hours. The number, size, distribution, and variety of resulting colonies were noted. A representative of each type of colony was selected to be Gram stained using the standard technique., Results: Of the plates, 42.2% were uncontaminated, 57.8% yielded bacterial colonies, and 15.6% of those colonies demonstrated hemolytic growth. The hemolytic growth included combined alpha and beta hemolysis and beta only hemolysis. Six colonies were gram-positive rods and 7 were gram-positive cocci., Conclusion: Meticulous infection-control techniques are inevitable and continuous reinforcement and training for staff and students are mandatory. Periodic gas sterilization of phosphor plates may be necessary.

Objective: This study aimed to investigate the fractal dimension values of trabecular bone in periapical lesions and healthy mandibular first permanent molars, based on age and gender, using panoramic radiographs. Material and Methods: The panoramic radiographs of a total of 216 patients aged 6-16 years who applied to Dicle University Faculty of Dentistry Department of Pedodontics in 2022 were retrospectively examined. The patients were equally distributed by gender into three age groups: 6-9, 10-12, and 13-16 years. Fractal analysis was applied using the ImageJ program to the regions of interest (ROI’s) of 25x25 pixels determined from two different regions on each digital panoramic radiograph, and fractal dimension calculations were performed. The data were analyzed using the Shapiro-Wilk and/or Kolmogorov-Smirnov tests, as well as the Mann-Whitney U and Kruskal-Wallis H tests. The confidence interval for all statistical tests was set at 95%, and the significance level was accepted as p<0.05. Results: According to the results of the study, the average fractal dimension value of the lesioned region was 1.106, while the average fractal dimension value of the healthy region was 1.116. No statistically significant difference was found in the fractal dimension values between both the lesioned and healthy groups, as well as between the age and gender groups (p>0.05). Conclusion: Fractal dimension (FD) calculation is a reliable method for detecting early-stage periapical lesions. However, to achieve more comprehensive evaluations, future studies should integrate histological and clinical parameters and compare 2D and 3D imaging techniques with larger sample sizes. [ABSTRACT FROM AUTHOR]

Objective The aim of this retrospective study was to compare the effects of the Twin block and Herbst appliances on the mandibular trabecular pattern using fractal dimension analysis (FDA) of panoramic radiographs (PRs). Methods The PRs of 50 subjects with skeletal Class II malocclusion who underwent the Twin block (T-group, average age: 11.63±0.87; 25 girls, 25 boys), 50 subjects with skeletal Class II malocclusion who underwent the Herbst (H-group, average age: 11.72±0.91; 27 girls, 23 boys), and 50 controls (C-group average age: 11.67±0.83; 24 girls, 26 boys) were selected. The condyle, corpus, and angulus regions of all groups in the mandible were examined using FDA. Results The condylar region (p≤0.001) and corpus mandible in the treatment groups (T-group: right, p≤0.05, left, p≤0.01; H-group: p≤0.05), as well as the left and right condylar region (p≤0.001) and left corpus mandible (p≤0.05) in the C-group, all indicated substantial increases in FDA between T0 and T1. Inter-group comparisons indicated that the T-group had greater variances in the condyle (p≤0.001) compared to the H group. Conclusion As the findings revealed both Twin block and Herbst appliances not only contributed to the dentoalveolar structure but also provided remodeling of the mandibular trabecular structure. Consequently, the null hypothesis was rejected. Keywords:Functional, Cl II, fractal, dental radiography: [ABSTRACT FROM AUTHOR]

Objective: The purpose of this research project was to investigate the origin of the anatomical structures interpreted as trabecula bone on dental radiographic images., Study Design: Mandible sections were cut sagitally into halves. Trabecular bone was removed from each section in 4 stages. Following each stage, standardized radiographs were made, using CDR direct digital equipment. Trabecular bone in the resulting digital images was measured with 4 methods: (1) mean gray level; (2) the fractal dimension of the basic images; and, following morphological image processing, (3) counting the number of trabecular ends, intercepts, and segments (EIS) and (4) performing fractal analyses of the skeletonized images. Additionally, human visual interpretation of the collected images was conducted through a written examination. Repeated measures analysis of variance (ANOVA) was used to test for changes in measurements attributable to bone removal., Results: Repeated measures ANOVA indicated that the use of gray levels, fractal dimension, and morphologic operations quantifying using EIS or fractal analysis had similar performance and resulted in significant changes in measurements following bone removal ( P < .05). Visual differences were not always apparent between each stage of bone reduction. Radiometric and morphologic analysis showed measurable differences between stages., Conclusions: These results imply that the inner trabecula, the junctional trabecula, and the actual cortical housing all contribute to some extent to the radiograph, although changes in the radiographic architecture are not always clinically detectible.

Background: The purpose of this study was to investigate the source of radiographic trabecular patterns by removing trabecular bone in four sequential steps from six cadaver mandible sections, radiographing the sections after each removal, and using four digital-image analysis methods to quantify any resulting changes to the radiographs., Methods: Mandible sections were cut sagittally into halves. Trabecular bone was removed from each section in four stages. Following each stage, standardized radiographs were taken, using direct digital equipment. Trabecular bone in the resulting digital images was measured with four methods. Mean gray level values (method 1) and cumulative percent histograms (method 2) were calculated from the raw data. Morphological image processing was used to skeletonize the trabecular structure, which was quantified by counting the number of trabecular ends and segments in the skeletonized images (method 3) and performing fractal analyses of the skeletonized images (method 4). Repeated measures analysis of variance (ANOVA) was used to test for changes in measurements attributable to bone removal., Results: Repeated measures ANOVA indicated that the use of gray levels, cumulative percent histograms, and morphologic operators resulted in highly significant changes in measurements following bone removal (P < 0.01). Ends and segments demonstrated similar performance, with changes highly significant over time (P < 0.01). Fractal analysis also resulted in highly significant changes over time (P < 0.01)., Conclusions: The analyses performed in this study demonstrated consistent image differences following the four steps of bone removal. These differences appeared whether light, cancellous bone or heavier endosteal bone was removed. These findings indicate that trabecular and endosteal bone combine to form the structure that most dentists identify as trabeculae on intraoral radiographs.

Objectives: The purpose of this research was to examine the influence of enamel matrix proteins (EMP) on the viability, proliferation, and attachment of periodontal ligament fibroblasts (PDLF) to diseased root surfaces., Materials and Methods: Primary cell cultures of PDFL were obtained from clinically healthy third molars or premolar teeth. Viability and proliferation rates were carried out over a 10-day period. A total of 80,000 cells were plated in 24-well plates followed by EMEM with 10% FBS (positive control) and EMEM plus EMP at 25, 50, 75, and 100 micro g/ml. Cells were harvested on days 1, 3, 5, 7, and 10 and viability was performed utilizing an MTS assay. PDLF proliferation rates were assessed by a CyQUANT GR dye assay. SEM analysis was used to examine the qualitative effects of cellular attachment to diseased root surfaces following EMP compared to nontreated controls., Results: The results indicated that viability was negatively affected for higher doses over time while lower doses displayed viability effects similar to control. Proliferation, however, appeared to be ameliorated following exposure to EMP. The SEM analysis suggests that cellular attachment to diseased dentin was enhanced following EMP application., Conclusion: These in vitro studies support the concept that EMP may act as a suitable matrix for PDLF.

Background: The loss of ovarian function at menopause is associated with loss of postcranial and oral bone. Hormone/estrogen replacement therapy (HRT/ERT) has a positive effect on both postcranial and oral bone. The objective of the study was to determine if the positive effect of HRT/ERT on alveolar crest height (ACH) is generalized or site specific., Methods: The sample consisted of 49 women who completed a 3-year, HRT/ERT prospective study. Cemento-enamel junction distances (ACH) were measured on digitized images of bitewing radiographs. Lumbar spine and proximal femur bone mineral densities (BMDs) were determined with dual-energy x-ray absorptiometric scans. Measurements were made at baseline and at the end of year 3. For the 3-year study period, mean change in ACH was determined for each patient. In addition, the sites with the greatest, second and third greatest ACH changes were determined for each patient. Correlations between changes in ACH (as determined by the various methods) and postcranial BMD were determined., Results: Mean ACH changes had an average correlation (r) of -0.24 with femoral and lumbar spine BMDs. Although the largest site-specific change in ACH resulted in a mean correlation of -0.21, the correlations for the second and third largest changes in ACH dropped to -0.15 and -0.12. Overall, the correlations for site-specific changes were substantively smaller than those for generalized change., Conclusions: The data of this study indicate that ACH change attributable to HRT/ERT is generalized rather than site specific. Studies of the effect of HRT/ERT on ACH should employ multiple measurements to minimize measurement errors associated with site-specific measurements.

Treatment planning is a critical aspect of clinical education in the dental school curriculum. It is surprising, therefore, that so little attention has been given to this subject in the dental literature. The importance of treatment planning is reinforced in the standards and the tests that clearly present methods and necessity for treatment planning. However, there is minimal evidence about how these treatment planning courses have been evaluated, how they were incorporated into the curriculum, or how they have been integrated into treatment planning in the academic clinical setting. The purpose of this study was to survey and profile current treatment planning processes in U.S. dental schools. A questionnaire consisting of twenty-nine items relating to treatment plan preparation, process, and outcomes was mailed to fifty-four U.S. dental schools. The primary topics included patient assignments, treatment planning, plan sequencing, plan presentation, informed consent, and plan modifications. Forty-seven of the fifty-four U.S. dental schools (87 percent) completed and returned the surveys. Profiling the treatment planning process in dental schools reveals many similarities. Typically, the schools screen patients prior to assignment to students and expect the student diagnostician to complete the planning process as well as comprehensive care. The patient's welfare is the primary determinant of the content of the plan in 92 percent of U.S. dental schools. Secondly, though current accreditation standards are concentrated on competencies, the treatment plans are influenced by quantitative requirements. Third, the plan is usually completed during the second patient visit after screening. Fourth, the approaches vary among the schools when a multidisciplinary or complex treatment plan is appropriate. Some depend on a panel of experts, whereas others do not have interactive planning with specialists. A significant number of schools decentralize treatment planning and delegate part of the plan to disciplines or group practice leaders. Fifth, the treatment plans and treatment risks are presented in accordance with the intent of the accreditation guidelines; however, fewer than half the schools explain the risk of procedures to patients at the time of plan presentation. Finally, plans change frequently, but the modifications are generally considered to be minor.

The American Academy of Oral and Maxillofacial Radiology developed these Parameters of Care to provide national guidelines for the use of radiographs prescribed for the diagnosis of disease, treatment planning, and follow-up care of patients with abnormalities of the oral and maxillofacial region. The Parameters cover radiographic techniques, imaging of the temporomandibular joint, imaging of diseases of the jaws, and imaging of dental implant sites.

Background: This study was undertaken to compare 5 digital analytic protocols for their abilities to extract data from digital clinical radiographs and discriminate between patients with gingivitis and periodontitis., Methods: Five digital-image analysis protocols were compared for their abilities to discriminate between two groups of 24 patients each. One group was diagnosed with healthy gingiva (or gingivitis) and the second with periodontitis. These groups were previously evaluated in published studies that used fractal and morphologic analyses. Pre-existing clinical radiographs for each patient were digitized and regions of interest (ROIs) were placed on interdental bone in mandibular posterior quadrants. The 5 protocols used were: 1) MGB: a median filtration to remove high-frequency noise, a Gaussian filtration to remove low-frequency noise, binarization of the resulting image, and quantification of the black pixels; 2) MGBS: the same protocol as MGB except for a skeletonization of the binary image and a quantification of the skeleton's pixels; 3) GBS: Gaussian filtration, binarization (thresholding on the mean pixel value) of the resulting image, skeletonization, and quantification of the pixels of the skeleton; 4) NS: normalization, skeletonization, and quantification of the skeleton's pixels; and 5) S: a variation of NS, except normalization was not used. The resulting values for the 2 patient groups were compared with Mann-Whitney U tests and effect likelihood-ratio test., Results: For digitized radiographs, the mean gray-scale value (+/- standard deviation) for gingivitis patients was 183.22 +/- 18.53 and for periodontitis patients 181.26 +/- 17.20. Mann-Whitney U tests resulted in the following P values for these protocols: MGBS <0.01; S <0.01; GBS <0.01; NS <0.01; and MGB <0.83. Effect likelihood-ratio tests indicated that only MGBS and S significantly contributed to models containing the other factors., Conclusions: Small variations to protocols affected the strength of the discrimination between the gingivitis and periodontitis groups. While there is potential for morphologic analysis to be used to discriminate between patients with gingivitis and periodontitis, a robust technique was not identified.

Ideal orthodontic treatment should achieve long-term stability of the occlusion. The mandibular incisor segment has been described as the segment that is most likely to exhibit relapse after treatment and retention. Therefore, relapse of this is a challenge that clinicians need to address. The purpose of this study is to evaluate the amount of relapse that may occur in Angle Class II Division 1 patients, treated orthodontically with tandem mechanics. All cases in this study were treated without extraction of permanent teeth, and the patients were followed for at least 2 years after the end of the retention phase of treatment. Six predictors were investigated at pretreatment, posttreatment, and postretention periods. A synopsis of this study shows the correction of lower incisor crowding as measured by the irregularity index was stable over 5.2 years of postretention follow-up; but longer follow-up time revealed increased relapse of incisor irregularity. Intermolar width increased during treatment and remained stable in the follow-up period. Overjet and overbite corrections and changes in the lower incisor to mandibular plane angle were also stable in the follow-up period. In addition, the amounts of overjet correction and loss of expansion of intercanine distance after treatment were associated with increased irregularity index in the follow-up period. It appears the discrepancies between this and previously published works are sufficiently dramatic that the whole question of treatment philosophy and long-term stability may need to be reevaluated.

Background: Our objective was to test the association between cemento-enamel junction, alveolar-crest distance (CEJ-AC, as measured on digitized vertical bite-wing radiographs) and postcranial bone mineral density (BMD) relative to clinical, dietary, and demographic variables., Methods: Data were collected in a cross-sectional study of 134 postmenopausal women. CEJ-AC distances were determined from digitized vertical bite-wing radiographs. Lumbar spine and proximal femur BMDs were determined from dual-energy x-ray absorptiometric scans. Correlation analysis and Student t tests were used to identify those variables most associated with CEJ-AC distance. The selected variables were modeled with a backward stepwise regression analysis, with CEJ-AC distance as the dependent variable., Results: Parity (number of pregnancies to term), cigarette smoking, and the interaction of lateral spine BMD with cigarette smoking were independent predictors of CEJ-AC distance (P < or =0.05). Statistical models containing these variables accounted for 19% of the variation in CEJ-AC distances., Conclusions: CEJ-AC distance in postmenopausal women is the result of a complicated interaction of many effects, including but not limited to, parity, cigarette smoking, and skeletal BMD.

Background: We set out to determine whether morphologic measurements extracted from digitized images of bite-wing radiographs correlated with lumbar and femoral bone mineral density (BMD) measurements in 45 postmenopausal women who had no or only mild periodontal disease (no probing depths >5 mm)., Methods: Lumbar spine and femoral BMDs were determined by dual-energy x-ray absorptiometry. Vertical bite-wing radiographs were taken and digitized. Crestal and apical regions of interest (ROIs) were drawn on the digital images of the maxillary and mandibular alveolar bone on the patient's right and left sides. For each patient, a single morphologic measurement was made for each of 8 ROIs. Correlation analysis was performed to determine the strengths of the relationships between the morphologic measurements made at the 8 locations and between these morphologic measurements and BMD measurements., Results: The correlations (r) between the morphologic operator (MO) measurements and lumbar spine and femoral BMDs were weak (mean r = 0.02, range = 0.32 to -0.26) and not statistically significant, with no clear trends discernible. Correlations between MO measurements made at the 8 alveolar sites were also weak (mean r = 0.05, range = 0.35 to -0.38) and not statistically significant., Conclusions: The MO measurements used in this study were weakly correlated with lumbar spine and femoral BMDs, with no clear trends discernible in this population of postmenopausal women with no or mild periodontal disease.

Objective: To determine the correlations among morphologic operations (MO) values and the correlations among gray-level values for regions of interest (ROIs) placed at various locations on digital images of alveolar bone for 45 patients., Study Design: As part of a larger study, up to 7 vertical bite-wing radiographs were taken and digitized for each of 45 patients. Sets of 2 rectangular ROIs were placed on the digitized images of interdental alveolar bone at 4 locations for each patient. The ROIs (1 crestal and 1 apical) were placed between second premolars and first molars in all 4 dental quadrants. Gray-level values were measured, and MO analysis was performed on each ROI. Descriptive statistics were calculated and correlations determined., Results: Paired correlations (such as apical vs crestal, left vs right, maxillary apical vs mandibular apical) of MO values were weak (r = 0.01-0.21), but corresponding correlations for gray-level values were relatively strong (r = 0. 60-0.92)., Conclusion: MO values varied with ROI location considerably more than did gray-level values. Additionally, ROI size and shape apparently affected MO data. Accurate placement and documentation of ROIs appear to be critical considerations in analyses that use MOs.

The correction and relapse of mandibular anterior crowding was evaluated in a population of 58 patients with Angle Class I malocclusion who were treated orthodontically without extraction of permanent teeth. The subjects were retrospectively evaluated from records taken before treatment, posttreatment, and postretention. The postretention period averaged 8 years (minimum of 4 and maximum of 20 years). All cases in Groups A and B were given orthopedic treatment to develop the maxillary apical base in the transverse and anteroposterior planes. Group A was treated with expansion of the inner bow of the face bow appliance (Kloehn), and Group B was treated with the Haas palatal expansion appliance. Both groups were then treated orthodontically with tandem mechanics. The response variables measured were: overbite, overjet, intercanine distance, intermolar distance, and irregularity index. Study groups A and B were not significantly different for subject age, retention, or postretention time. Moreover, the groups did not show significant difference for any of the response variables before treatment. However, there was a statistically significant difference in the treatment times (P =.0133). A statistically significant treatment effect was observed for most response variables in the groups. Overbite, overjet, and irregularity index were significantly reduced, intermolar distance was significantly increased, and intercanine distance showed no significant change in Groups A and B. In the postretention period, there was a tendency for variables to change slightly toward their before treatment values but no compromise of orthodontic correction was noted. The irregularity index in Group A was corrected from 4.8 to 1.1 mm and remained at 1.1 mm in the postretention period. The irregularity index in Group B was corrected from 5.1 to 1.2 mm (P =.0001) and changed slightly from 1. 2 to 1.7 mm (P =.0540) in the postretention period. We concluded that mandibular incisors tended to become more crowded postretention. However, in contrast to previous reports, we calculate this relapse to be small. Neither before treatment nor posttreatment variables were predictive of relapse.

Observer reliability in performing linear measurements between the cementoenamel junction and alveolar crest was determined for mandibular posterior teeth from digitized clinical bitewing radiographs acquired during recall examinations. 6 measurements (corresponding to traditional probing measurements) were made per tooth by 3 observers. Mesial and distal measurements made to the most coronal aspects of the alveolar crest were the most reliable and least biased. As was anticipated, intra-observer reliability was better than inter-observer reliability although the 3 observers of our study were able to detect a significant mean change (0.1 mm, p<0.0001) in alveolar bone height over a 1-year period for 10 patients. For our most reliable and unbiased measurements (mesial measurements to the alveolar crest), a change of 0.54 mm (90th percentile) would be required to indicate change at a site from one time to the next. Based on the reliability of our digital radiographic measurements, with the alpha error rate set at 0.05 and beta at 0.20, a difference in alveolar bone height of 0.3 mm could be detected with a patient sample size of between 13 (best case) and 54 (worst case).

Objective: To compare the reliability of radiodensity measurements made from dental radiographs with manual and a novel computer-intensive methods., Methods: As part of a prospective study of postmenopausal women, a series of seven vertical bitewing radiographs were taken of 36 patients. One of each set of radiographs was repeated. The original and the corresponding duplicate radiographs were used in this study. Radiographs were digitized at 50 microns spatial resolution and 12-bit gray-scale resolution. For the Manual Method, original and duplicate radiographs were manually cropped to improve image homology, histogram matched and mean pixel gray-scale values determined for an alveolar bone ROI within each image. For the computer-intensive method, images were put into registration with ANALYZE software (Mayo Foundation, Rochester, MINN, USA), cropped automatically, histogram matched and color-coded on the basis of the per cent difference. Alveolar bone ROIs adjacent to clinical crowns and root surfaces whose color code indicated less than a 5% change were sampled. Method error (ME) and the coefficient of variation of method error (CVME) were calculated., Results: With the Manual Method the SD between original and duplicate measures was 95.21 out of 4096 gray scale values; ME = 67.32; CVME = 3.78%. For the computer-intensive method, the corresponding values were 54.74, 38.71, and 2.29%., Conclusions: The new computer-intensive method resulted in a 40% improvement over the Manual Method in the precision of radiodensity measurements.

Objectives: This study was conducted to determine whether morphologic operation procedures applied to digitized, non-standardized, clinical radiographs of mandibular alveolar bone could be used to distinguish between a population of patients diagnosed with periodontitis and a population of patients either diagnosed with gingivitis or having healthy gingivae., Study Design: Two groups, one consisting of 29 patients who either had healthy gingivae or had been diagnosed with gingivitis and the other consisting of 32 patients who had been diagnosed with periodontitis, were compared. Pre-existing clinical radiographs were digitized, and for each patient three to six regions of interest were placed on an image of the mandibular posterior region of the interdental bone. The regions of interest were processed under two morphologic-operations protocols, and a mean density (referred to as an MO number) was calculated for each patient. With paired t-tests, the resulting MO numbers for the two groups were compared., Results: The two populations were statistically different (p < 0.05)., Conclusion: The results of this study indicate that morphologic operations have the potential to differentiate between patient groups differing in periodontal health.

This study was undertaken to demonstrate that the fractal dimensions calculated using digitized non-standardized, clinical radiographs of mandibular alveolar bone from a population of patients diagnosed with periodontitis are statistically different from fractal dimensions calculated from another population diagnosed as having gingivitis or healthy gingiva. The fractal dimension was calculated using a public domain fractal analysis program distributed by the National Institutes of Health (NIH). Fractal dimensions were calculated from digitized clinical radiographs for 29 patients diagnosed with healthy gingiva and/or gingivitis and 32 patients diagnosed with periodontitis and compared. To estimate the reproducibility of the technique, we recalculated the fractal dimension from images of the gingivitis patients 3 months after the original calculations and compared them to the originals. A 2 sample, 2-tailed Student t test showed the gingivitis data group to be different from the periodontitis data group (P = 0.0012). The original gingivitis and repeat gingivitis groups fractal dimension calculation were the same and analysis showed the two data sets were not significantly different (P = 0.99). We found that: 1) fractal dimensions could be used to distinguish between gingivitis and periodontitis patient groups; 2) fractal dimensions could be calculated from non-standardized clinical radiographs; and 3) fractal dimensions for gingivitis patients were reproducible over a 3-month period.

Objectives: The purpose of this study was to evaluate the ability of two different panoramic imaging systems to produce cross-sectional images with accurate vertical dimensions of the posterior mandible., Study Design: Three partially edentulous human cadaver mandibles were used for this study. On each mandible, three potential implant sites were arbitrarily identified in an area between the mental foramen and the ascending ramus. Each site was imaged using two different panoramic machines. Using each image, the mandible's outline, cortical thickness, and position of the mandibular canal were traced on clear acetate film. The mandibles were then sectioned at each site to serve as a gold standard. The cadaver sections and tracings (corrected for magnification) were measured, recording the overall mandibular height, distance from the crest of the ridge to the superior aspect of the mandibular canal, and the thickness of the cortical bone at the most inferior aspect of the mandible., Results: There were no significant differences between either of the system's image measures and the gold standard when considering the distance between the crest and the mandibular canal. Differences were noted between the systems measures and the gold standard in the assessment of the cortical bone thickness and the overall mandibular height., Conclusions: Both imaging systems can be useful for vertical measurements of a potential implant site in the posterior mandible.

To determine whether postmenopausal bone loss and factors associated with osteoporosis affect tooth retention, we examined vertebral and proximal femoral (postcranial) bone mineral density in relation to tooth loss and attachment loss in a cross-sectional study of 135 postmenopausal women (age range 41-70 yr). Women had at least 10 teeth and no evidence of moderate or severe periodontal disease. Full-mouth attachment loss measurements were made using a pressure-sensitive probe, and bone density was determined by dual-energy X-ray absorptiometry. Attachment loss was correlated with tooth loss (number of remaining teeth, radiologically determined), but not with vertebral or proximal femur bone density. Multivariate analysis showed current smoking (p = 0.01), years since menopause (p = 0.02) and the interaction of age and current smoking (p < 0.01), to be statistically significant predictors of attachment loss in our study population.

Objectives: To compare the effect of using regions of interest (ROIs) of different size and shape on the fractal index of alveolar bone., Study Design: Two sets of clinical posterior bitewing radiographs were used to calculate the fractal index (S). Two comparisons were made. First, S was calculated from large interdental ROIs that included small amounts of root structure and compared with S from small ROIs that included no root structures. Then S was calculated from large interdental ROIs (similar to those used for the first set) and compared with S calculated from ROIs that included nearly all of the mandibular alveolar bone (and adjacent root) present on the bitewing., Results: For the first comparison, paired t-tests showed that fractal indices calculated with the large ROIs were significantly different from the respective indices calculated from the small ROIs (P < 0.001). For the second comparison, the fractal indices calculated from the large quadrant ROIs were not significantly different from those calculated from the large ROIs (P = 0.120)., Conclusion: ROI size and shape may affect the results of fractal analysis of alveolar bone.

Objectives: We used digitized dental radiographs of alveolar bone to test the hypothesis that the fractal dimension, as calculated with the program "ImageFractal" was independent of variations in X-ray exposure, beam alignment, and region of interest placement., Study Design: The radiographic data set consisted of 72 radiographs digitized with 200 microns pixels. Radiographs were obtained with the use of three time settings and two alignments. Rectangular regions of interest were placed on each digital image over the interdental bone between the mandibular first and second molars on six hemimandibles. Each of six hemimandibles had identical copies of a unique region of interest placed on every image in its series. New regions were made 3 months later. A fractal dimension was computed from each region of interest with the caliper method included in ImageFractal, a public domain program available through National Institutes of Health. The resulting fractal dimensions were evaluated with two repeated measures analysis of variance., Results: No significant differences were found between the fractal dimensions calculated for baseline images and those from overexposed and underexposed images, from images with 4 to 6 degrees of alignment variations, or from repeat regions of interest., Conclusion: The results support the hypothesis that fractal dimensions derived from digitized dental radiographs are not affected by variations in exposure or small variations in alignment and imply an absolute region of interest placement may not be necessary. However, caution should be used with the use of the fractal dimension to discriminate among alveolar bone variations until further research is performed.

Unlike traditional radiographs, digital images are electronically alterable and offer the potential for enhancing diagnostic information. The authors conducted a small-scale study to examine differences in clinicians' diagnoses of caries using traditional radiographs and digitized images vs. microscopic diagnosis. Two general dentists and one oral-maxillofacial radiologist scored the images for caries. This study suggests that digital enhancements may aid some clinicians in caries diagnosis.

This pilot study was undertaken to determine whether spatial resolution affects radiometric analyses aimed at detecting progressive enamel loss. Four teeth were weighed, attached to a positioning device, and evaluated with radiography. A 1 mm strip of enamel was removed from each tooth, and the teeth were weighted again and reexamined by radiography. This process was repeated five times until 1/2 mm of dentin was removed. The radiographs were digitized twice with 59 and 200 microns pixels at 8 bits, providing two series of images with the optical densities converted into 256 gray levels. Each series of images was adjusted for contrast variation. Regions of interest were drawn on the crowns, and cumulative percent histograms (CPHs) were calculated. Within a series of CPHs enamel reduction resulted in shifts in the CPHs that were directly proportional to the amount of enamel removed. CPH shifts associated with the smaller 59 microns pixels accounted for 68% of the variation in weights caused by enamel reduction, whereas the shifts associated with the larger 200 microns pixels accounted for 50%. The results indicate that pixel size does affect radiometric determinations of enamel reduction.

Objectives: To compare the results or two methods of histogram matching and two methods of histogram flattening for their ability to correct for contrast variations in digital dental images., Methods: A custom-built, aluminium stepwedge with 0.1, 0.5 and 1.0 mm steps was placed over Ektaspeed films and exposed for 0.06, 0.12 and 0.25 s, respectively. Radiographs were digitized at 50 microns spatial resolution and 12-bit contrast resolution. Contrast corrections were performed using Rüttimann et al.'s algorithm (1986) for one method of matching (RM) and flattening (RF) and Castleman's algorithm (1979) for the other method of matching (CM) and flattening (CF). Mean pixel grey-scale values were determined for each step. The 0.12 s exposure was considered to be the target image exposure. Absolute differences in pixel grey-scale values between the target images and the modified images were determined., Results: The median values of the absolute differences in pixel grey-scale values between the target images and the contrast corrected images were: CM = 4.3; RM = 4.1; CF = 70.2 and RF = 70.2., Conclusion: Castleman's and Rüttimann's matching algorithms perform equally well in correcting digital image contrast. Histogram flattening was less effective.

This pilot study was undertaken to determine the effect of x-ray beam alignment and spatial resolution on quantification of alveolar bone using radiometric techniques. Six (6) dry mandibles were radiographed at 70 kVp, 10 mA, 0.6 seconds using D-speed film, with a bone chip (2.64, 4.10, or 6.07 mg) present or absent at 7 x-ray beam alignments (0 degree, 2 degrees horizontal, 2 degrees vertical, 4 degrees horizontal, 4 degrees vertical, 6 degrees horizontal, 6 degrees vertical). This resulted in 28 radiographs per mandible. Radiographs were digitized using 50- and 200-microns pixel spatial resolution. Image gray levels were standardized using a simple look-up table shift. Regions of interest (ROIs) were positioned on the alveolar bone where the bone chips had been placed. Cumulative percent histograms (CPH) were calculated for those ROIs. Regression analysis was used to evaluate the relationships between CPH changes and bone chip size as x-ray beam angulation and spatial resolution was varied. The resulting R2 values for angulation ranges of 0 degree to 1.4 degrees, 1.5 degrees, to 2.4 degrees, and 2.5 degrees to 5.5 degrees were: 0.983, 0.941, 0.891 for 50-microns pixel images and 0.869, 0.909, and 0.774 for 200-microns pixel images. We conclude that 50-microns pixel spatial resolution is apparently superior to 200-microns pixel images if radiometric data is to be evaluated. With 50-microns pixel spatial resolution, alignment variations up to 5 degrees may be acceptable in clinical studies, depending on the magnitude of bone change that is to be detected.

Objectives: Radiometric analysis can be used to identify small changes in grey levels taken from investigator-defined regions of interest (ROIs). To do this, standardization of the radiographic optical densities and image grey scales is considered essential. Rigid standardization is one of the impediments to applying existing radiometric techniques to clinical practice. The purpose of this study was to evaluate the use of grey-level variations from a series of intrafilm control regions of interest (ROIs) to adjust radiometric grey-level data taken from test ROIs., Methods: After digitization, ROIs were drawn on three locations: the background, anatomical crown, and tooth root. Mean grey levels, histograms and cumulative percent histograms (CPHs) were determined for each group. With the goal of decreasing the distribution of these curves from their unadjusted grey-level positions to zero (superimposing all curves), shifts required to align the control CPHs were applied to test CPHs. Changes between the pre- and post-adjusted CPH distributions were measured., Results: Intrafilm controls provided a combined decrease of 45.3% in the grey-level distribution error., Conclusion: While CPHs can be adjusted using intrafilm controls, these adjustments may have limited benefit.

Longitudinal radiographic assessment of crestal alveolar bone plays an important role in the diagnosis and long-term evaluation of periodontal disease in patients. Because practitioners use several radiographic techniques to obtain bite-wing radiographs, horizontal and vertical alignment errors could adversely affect the diagnostic impression gained from this type of radiographic examination. The objective of this study was to determine the alignment reproducibility of three different clinical techniques used to acquire bite-wing radiographs. Patients who require bite-wing radiographs as part of a dental school screening process were radiographed with modified standard bite-wing tabs and two different intraoral positioning devices. Horizontal and vertical angular deviations were measured and alignment errors were calculated for each radiograph. The mean total angular alignment error for the standard bite-wing tab technique was 6.2 degrees, whereas the mean alignment error for both positioning devices was less than 1.8 degrees. The results of this study suggest that an intraoral positioning device for acquiring bite-wing radiographs should be used.

The resolution requirements were determined for detection of incremental alveolar bone loss from digitized Ektaspeed radiographs. Ten clinical radiographs were examined with a calibrated optical microscope to measure the smallest feature of interest discernible for alveolar bone. Images of trabeculae > 100 microns in diameter could be identified, but smaller ones could not be resolved. The Nyquist Criterion requires sampling with 50 microns (or smaller) pixels to measure such features. Numerous 25 microns Ag aggregates were present. Fifty microns resolution is a practical compromise between noise level and feature resolution. In another example of 10 bitewings digitized at 8 bit grey scale, about half the available range was used for alveolar bone, resulting in uncertainty, over a range of 2 optical density (OD) units, of about 1.42% at the average OD for alveolar bone (1.1). With the same radiographs digitized at 12 bit resolution, over 2000 of 4096 grey scales were used with a corresponding uncertainty of about 0.09%. Twelve-bit precision was also able to resolve smaller increments in an aluminium stepwedge than was 8 bit precision. Twelve-bit grey scale and 50 microns spatial resolution are recommended for alveolar bone densitometry performed with Ektaspeed radiographs.

Orthopedic surgery and general dentistry residency program directors were surveyed about treatment considerations for dental patients with prosthetic joints. The majority believed dental diseases can affect joint prostheses and thought orthopedic surgeons should be consulted before dental treatment. Both groups recommended antibiotic prophylaxis for these patients.

In vitro periodontal alveolar bone mineral content (BMC) measurements obtained with dual-energy radiography (DER) were compared with assessments based on bitewing radiographs (BWX). In addition, in patients, the relationship between bitewing and several postcranial dual-energy-radiographic measures were evaluated. Dual-energy-radiographic and bitewing measurements were made on 2 cadaver mandibles initially and after 2 incremental bone reductions at 4 sites. Rank-order correlations between dual-energy-radiographic and bitewing measures for the 4 sites ranged from 0.7 to 1.00. Bitewing measures indicated true bone loss with a sensitivity of 1.00. For patients, correlations between bitewing measures and dual-energy-radiographic scans suggested the strongest relationships were in the distal sections of the radius and ulna and in the intertrochanteric and Ward's areas of the femur. Correlations, in the 0.5-0.6 range, were not statistically significant (p > 0.05), but were quite robust considering the small sample size and preliminary nature of this investigation. Results suggest that the bitewing measure is sufficiently sensitive to detect clinically meaningful (5% or greater) changes in alveolar BMC and, further, that alveolar bone mineral content may reflect postcranial BMC. The implications of postcranial bone mineral changes being reflected in alveolar bone would enhance both our understanding and treatment of alveolar bone loss. The use of bitewing measures to facilitate identification of patients with postcranial bone loss is discussed.

A 35-mm slide scanner digital imaging system was tested for its suitability in digitizing intraoral dental radiographic film for quantitative studies. The system (Nikon model LS-3510AF Nikon Electronic Imaging, Nikon, Inc., Melville, N.Y.) uses a charge-coupled device linear photodiode array. The data content in the original film images was evaluated, and the system performance assessed objectively with the use of specially designed test films. Radiometric and geometric performances for the digitizing system were extracted from measurements and observations, and these were compared with published data for two other film digitizing systems (video camera DAGE MTI, Michigan City, Ind. and Barneyscan 35-mm film digitizer Barneyscan, Berkeley, Calif.). The techniques used to evaluate this system are easy and suitable for evaluation of any digitizing system. This scanner system (Nikon) was superior to previously evaluated systems in transforming and recording radiographic film densities across the range (0.3 to 2.0 optical density units) of clinically relevant optical densities. The scanner offers substantial advantage over the other digitizing systems for gray scale information from clinically important optical densities.

Typical 35-mm slide scanners use a photodiode array and software that allows for digital and analog controls that are manually adjustable. The digital controls provide brightness and contrast adjustments, whereas corresponding analog controls adjust the exposure time and black level that determines the clamping level of the charge-coupled device for the maximum black in the image. The objective of this study was to determine the effects of these controls on the radiometric data of intraoral dental radiograph images, to establish recommended settings, and to set specific standard guidelines for the digitization process. Three approaches were used. The results of this study demonstrate that brightness and contrast control alterations on the digitizer produces different optical densities and modulation transfer function values. The impact of these results is unresolved yet must be considered in analysis on quantitative radiometric studies.

It is generally accepted that two of the most commonly seen radiolucencies, the periapical granuloma and the radicular cyst, cannot be differentiated radiographically. The purpose of this investigation was to determine the feasibility of identifying radiometric differences in these two pathologic conditions by analyzing the patterns of the gray-level distributions of digitized radiographic images. Radiographs used in the study had been sent by practitioners to the School of Dentistry with tissue specimens. Radiographs of lesions identified as periapical granulomas and radicular cysts in the mandibular posterior quadrant were included in the study. The radiographs were digitized and regions of interest were drawn to include the lesions. Frequency distributions (histogram) with 256 gray-scale intervals and a cumulative percent histogram were computed. The typical histograms of the dental granulomas had narrower ranges and lower gray-scale values than did cysts. The results of this study indicate that it is feasible to radiometrically differentiate between lesions that are visually indistinguishable on radiographs.

Previous studies have demonstrated that accurate alveolar bone-level measurements can be made using digitally enhanced bitewing radiographs. In this study, we determined the effect of exposure variations on bone-level measurement accuracy using digital radiographic images of dry skulls. 6 direct bone-level measurements on the mandibular 1st molars of dry skulls were established by consensus of 2 expert observers. 13 bitewing radiographs were taken on each side of 11 skulls, using a specially designed positioning device. Exposure settings of 70 kVp, 10 mA, and 24 impulses were empirically chosen as the baseline. Kilovoltage and impulses times were individually varied (50 to 100 kVp in 10 kV increments and 15 to 48 impulses in 6 steps) to ensure a wide range of clinically relevant optical densities on the test images. The radiographs were digitized and alveolar bone level measurements, corresponding to those for skulls, were made on the images by 2 observers. Pearson product-moment coefficients between radiographs and criteria measurements were calculated. It was found that dry skull and digital bitewing radiographic measurements were highly correlated, within the limits of exposure parameter variations tested. Accurate measurements of alveolar bone level are possible from bitewing radiographs taken within a wide range of exposures, when digital image processing techniques are employed.

Normalized cumulative percentage gray-scale distributions from digitized radiographs of dry skulls can be used to evaluate alveolar bone health. With this method, manually defined regions of interest are used to identify the area of the image to be analyzed. The purpose of this study was to determine if variations in the dimensions of the regions of interest affect the gray-scale distributions. Two approaches were used. The effect of region of interest outline variations on (1) the ability to differentiate between various sized bone chips and (2) the ability to distinguish between two groups of skulls with the alveolar bone subjectively rates as normal or diseased were investigated. Four different regions of interest, which consisted of outline and area variations to a basic region, were used. Ninety-one percent of the cumulative histograms that represented chips were sufficiently separated from nonchip plots to be distinguished. Bone health was correctly assessed with a numeric classifier for 37 of 44 quadrants, for an accuracy of 84%, a specificity of 79%, and a sensitivity of 90%. Although ROI size and shape consistency in longitudinal studies are important in radiometric analyses, small variations had minimal impact in this study.

A survey of 97 dentists who had recently taken a state dental board examination was completed to document the use of radiographs for the screening and treatment of their patients. The response rate was 53%. Candidates spent on average 14.25 hours screening a mean of 18 patients to identify the dental pathoses necessary to take the examination. Candidates took a mean of 28.9 (+/- 25.8) radiographs for screening purposes and during the examination with a range of 3 radiographs to a maximum of 141 radiographs. Time in practice and specialty status did not affect the total number of radiographic exposures made. The majority of radiographs taken (53%) were unlikely to be used in the direct care of the patient's dental needs. The results of this survey indicated that efforts to minimize administrative radiographs should be increased.

Errors in X-ray beam alignment account for many of the exposure errors in bitewing radiography. A new method for measuring these alignment errors is described. The alignment accuracy of two different models of inexpensive plastic positioning devices, used for bitewing radiography, were quantified and compared. The technique involves documenting the alignment of an 'ideal' reference radiograph and successive exposures. It permits calculation of X-ray beam-film alignment errors in all subsequent radiographs using the 'ideal' reference. Bitewing radiographs were taken on 61 patients and 19 of these were re-radiographed at 6-month intervals over a 2-year period, providing a total of 156 radiographs for the study. Horizontal and vertical angular deviations were measured and total alignment errors calculated for each radiograph. The average angular alignment error was less than 2 degrees total angular error (1.3-2.4 degrees, 95% confidence intervals). It is concluded that these devices can be used when X-ray beam-film alignment error below 2.5 degrees is acceptable.

A radiograph-based classifier for discrimination between normal and diseased alveolar bone was developed. The alveolar bone health of dry mandibles was rated, by consensus of two trained observers, as normal or diseased. Bitewing radiographs of mandibular quadrants were digitized and regions of interest (ROIs) of the alveolar bone between the first and second permanent molars outlined. Gray-scale histograms for the ROIs were computed, and a classifier based on these histograms was developed. Two observers (independently) used only the classifier to rate the bone as being normal or diseased. These ratings were compared with the original ratings of trained observers who viewed and scored the actual alveolar bone. The sensitivity (the ability to detect diseased alveolar bone), specificity (the ability to detect normal alveolar bone), and accuracy were 0.90 or greater, indicating good agreement with subjective visual assessments of dry mandibles. These results suggest that this procedure may be applicable for evaluations of bone health in humans.

Dental radiographs are amenable to digital image processing and analysis for both subjective interpretation and quantification of scene features. Four general-purpose image processing programs for the Apple Macintosh II computer (NIH Image, Enhance, IP Lab and DIP Station) were applied to digital analysis of dental radiographs. These programs can manipulate and quantitatively analyse the gray scale and spatial data of dental images and improve their subjective quality. They can interactively make geometric measurements of features. The programs were evaluated for their versatility and applicability in the quantification of alveolar bone. Overall, NIH Image was judged most suitable for current dental imaging needs. Each of the other programs offered unique features that may be valuable in specific applications.

Alveolar bone loss measurements made using digitized dental radiographs have been shown to be precise and accurate. We determined the influence of alignment errors introduced when exposing the radiographs on the precision and accuracy of bone loss measurements. Bitewing radiographs of 15 day skulls were obtained using a positioning device (modified after the methods of Duckworth and associates). The sequence of radiographs was taken while the alignment of the main Roentgen beam was systematically varied within a 10 degree cone. Angular displacements were calculated from the digitized radiographs. Space variant digital image enhancement was performed to improve visualization of the cementoenamel junctions and alveolar crests. Corresponding bone loss measurements were made on dry skulls and radiographs. The criterion for truth in these measurements was established by 2 expert observers who measured the skulls with periodontal probes. Radiographic measurements were statistically compared with the criterion using validity coefficients. We found beam positioning errors of up to 10 degrees do not substantively affect alveolar bone loss measurements of mandibular molars taken from enhanced digital images of bitewing radiographs.