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Background: Astaxanthin (AST) shows a large range of beneficial effects together with anti-cancer and antioxidation properties. Human Serum Albumin (HSA) is the most abundant protein in blood plasma which plays the role of a depot and transport protein for many exogenous compounds. However, whether HSA could enhance AST-induced cytotoxic effects in human ovarian cancer cells has not been examined to date. Objective: This study aims to explore the anticancer effect and the molecular mechanism of AST combine with HSA induced cytotoxicity in ovarian cancer SKOV3 cells. Methods: The ovarian cancer SKOV3 cells were treated by AST combined with HSA to study the effects of cell proliferation, cell morphology, cell cycle arrest, related protein expression, nuclear transfer, cell migration, and drug-resistant. Results: Our data confirmed that AST+HSA treatment enhanced the anticancer effects of AST, arrested G1 phase cell cycle and induced apoptosis in SKOV3 cells. AST+HSA induced apoptosis via mitochondrial apoptotic pathways was related to the increased ratio of Bcl-2/Bax and activation of caspase-3. Besides, exposure of cells to AST+HSA triggered the inactivation of NF-κB and activation p53 and MAPKs signaling pathways. Furthermore, AST+HSA significantly overcome the drug-resistant and inhibited the migration of SKOV3 cells. Conclusion: AST combined treatment with HSA considerably inhibited NF-κB expression and translocation to nucleus, thereby improving the AST-induced cytotoxic effect on SKOV3 cells. These findings may provide rationale to combine AST with HSA for the treatment of ovarian cancer.

The performance and microbial characteristics of ammonium-limited and nitrite-limited ANAMMOX reactors were studied in two continuously stirred tank reactors. The influent TN concentrations were controlled below 50 mg·L

Aim: To investigate the relationship between PI3K/Akt/NF-κB cellular signal pathway and the expression of P-gp and LRP in multidrug resistance (MDR) cell of nasopharyngeal carcinoma. Method: The PI3K, p-Akt and NF-κB/p65 as the activity of PI3K/Akt/NF-κB were detected by Western blot. The expressions of LRP and P-gp were detected by Western blot and real-time PCR. Result: The RIs of CNE/DDP group to DDP, 5-Fu, VCR, ADR and PTX were 35.04, 18.14, 24.13, 12.00 and 10.18, respectively. The RIs of LY-294002 group were 11.77, 5.83, 3.07, 3.86 and 3.34, and PDTC group were 11.08, 6.55, 7.66, 2.18 and 4.05. The expressions of PI3K, p-Akt and NF-κBp65, LRP and P-gp were increased and mRNA of LRP and P-gp were up-regulated in CNE/DDP. The expression of p-Akt in LY-294002 group was down-regulated. The expression of NF-κB p65 in PDTC group was decreased. The mRNA of LRP and P-gp in LY-294002 group and PDTC group were decreased. Conclusion: MDR of nasopharyngeal carcinoma cell can be regulated by activating PI3K/Akt/NF-κB signal pathway and then increase the expression of P-gp and LRP. The MDR of nasopharyngeal carcinoma cell can be reversed by inhibiting PI3K/Akt/NF-κB signal pathway.

To evaluate the treatment effect of topical doxycycline on avulsed permanent teeth compared with normal saline.A total of 44 avulsed teeth from 38 patients (22 boys and 16 girls, aged 7-14 years) were recruited. Twenty-one teeth in group A were treated with doxycycline for 5 min before replantation while 23 teeth in group B were treated with saline solution. All participants were followed up for at least 12 months. The clinical outcome differences between 2 groups was evaluated by Mann-Whitney U test.In group A, 18 teeth were found pulp necrosis, 3 with infection-related resorption, 13 with ankylosis-related resorption and 6 were extracted. In group B, 16 were diagnosed with pulp necrosis, 4 with infection-related resorption, 12 with ankylosis-related resorption and 7 were extracted. No significant differences were found between the two groups on pulp survival and periodontal healing.Compared with treatment with normal saline, avulsed permanent teeth treated with doxycycline did not show a better clinical outcome.

Aiming at the center shaft bracket sheet extension parts, its stamping calculation is simulated based on the sheet metal forming with nonlinear finite element software Dynaform. The contours varying with blank holder forces, friction coefficients and material performance parameters (sheet hardening exponent n and thick anisotropic coefficient r) are obtained by using the simulation software. The impact of the factors on the central shaft bracket parts stamping is analysised by using the contours, provide the theory to eliminate the fold and fracture in the stamping process.

The highest frequency duct is evaporation duct under marine atmospheric environment, and it is a special method of expanding the radar measuring range by using the evaporation duct to achieve the over-the-horizon detection. On what conditions to ensure the realization of the over-the-horizon radar detection is one of key problems. Many aspects, such as evaporation duct discrimination, radar working wavelength and frequency, initial launching angle and the location of radar are studied to get the necessary conditions of the over-the-horizon radar detection. The results show that the four kinds of necessary conditions must meet at the same time instead of only one certain condition, which lays a necessary foundation for the marine radar, ship radar and communication system to exert the largest effects in the evaporation duct environment.

Combining the characteristics of silver inserts, it was tested that its molding process and impact strength from the PA1010 injection molding product's craft. A kind of effective method was found that make sure silver embedded parts not occur surface color as injection heat and determined the reasonable injection molding craft. Meanwhile measuring impact strength of the nylon 1010 injection molding products through the impact test, It was discovered that injection products could well meet the assembly convenient, reliable and the electric conduction needs after inserting the embedded parts in the PA1010 injection molding products. Against molding defects of PA1010 injection molding products and influencing factors of the impact strength, the corresponding analysis was made.

Based on parameters and design requirements of the original LJ276M gasoline engine, using Helmholtz theory to calculate resonance frequency, intake manifold length and diameter and other structural parameters, rapid design a new resonance intake system. And then apply simulation software GT-Power to build working process model of the LJ276M Electronically Controlled Gasoline engine, and determine the final structural parameters of the intake system through the use of rapid prototyping and test verification methods. Compared with the original machine, the dynamic performance is improved significantly by the use of optimum design of the intake system.

The impacts of multi-monomers melt grafting blends of maleic anhydride(MAH) and Styrene (St) on the crystallization behavior, morphology and mechanical properties of PA1010/ABS/PP blends were studied through the methods of SEM,themal analysis, mechanical properties analysis. The results showed that the graft copolymer generated in situ by the anhydride groups of g-PP and the amino end of PA1010 reduced the interfacial tension of blends efficiently, and the interaction of the styrene groups of the graft copolymer and ABS is helpful to improve the compatibility of three components during the melt blending process. The size of dispersed phase in blends is reduced obviously and the mechanical properties have a great improvement when the percentage of g-PP in the PA1010/ABS/PP blends increases to 10%. The tensile strength of the compatibilized PA1010/ABS/PP blends is improved by 11.7%,and the impact strength and elongation at break are increased 4 times and 4.9 times, respectively.

By Means of Fluent 6.3，the paper simulated the solid-liquid two-phase flow to a type of centrifugal sewerage pump under different sand size and contain arenaceous concentration conditions, using Eulerian Mixture Model, adopting SIMPLE algorithm, k-ε-Ap turbulence model and partitioning non-structure hex grid in the area of flow passage in the pump. The paper analyzed wear characteristics in different parts of centrifugal sewage pump impeller and put forward some means to decrease attrition for impeller. The results can be as theoretical reference for the optimization design of the centrifugal sewage pumps

The noise of exhaust muffler is one of the key factors of the noise of the whole car. As a key member in reducing the exhaust noise, its performance directly influence the smoothness of the car. By establishment of simulation model of coupling of engine working process and muffler the plug-in loss and pressure loss are obtained. The result of simulation calculation is basically the same as that of actual test. The measures to optimize the structure of the muffler proposed based on the study have achieved relatively satisfactory effect.

The use of L27 (35) orthogonal to the battery shell injection molding process is optimized. The main factors of technical parameters were determined mould temperature, melt temperature, the speed of injection, injection pressure, cooling time.On the basis of actual production, to determine the factors values of different process parameters.Combination of scrapped products in key (reduction and a high degree of tolerance deflated) tests were selected in the process parameters within the scope of the assessment. Various factors impact on the product of the total height followed by cooling time, mold temperature, melt temperature, injection pressure, injection speed from strong to weak .The best products technological parameters were determined.Good results were obtained for production.

Using GT-Power software to establish the simulation model of the working process of an engine coupled with the muffler, insertion loss and pressure loss are obtained, simulation results are consistent with the experimental results, which verifies the correctness of simulation model.Therefore, it is important to shorten the vehicle development cycle and reduce cost.

By Means of Fluent 6.3，the paper simulated the solid-liquid two-phase flow to a centrifugal sewerage pump，using Eulerian Mixture Model under different working condition and different particle size. The simulation draws some conclusion on distributive rules of solid particle inside impeller passage. The results for this simulation were as following: Distributive rules of solid particle inside impeller passage mainly relate to particle size. Meanwhile，the volume fraction of particles and operation condition have influence on distributive rules of solid particle. The simulated results can explain commendably that attrition took place inside pump passage when pump transported solid-liquid two-phase flow. Meanwhile，the simulated results have reference price to improve the design for pump and performance of pump.

Two new compounds, named (Z)-7,4'-dimethoxy-6-hydroxyaurone-4-O-β-glucopyranoside (1) and (-)-4-O-(4-O-β-D-glucopyranosylcaffeoyl)quinic acid (2), were isolated from the endophytic fungus Penicillium citrinum of Avicennia marina. Their structures were elucidated on the basis of spectroscopic analysis. Additionally, compound 2 exhibited potent chemoreversal activity, mainly by inhibiting P-glycoprotein efflux pump function.

To evaluate the effect of amount of silane coupling agent on flexural strength of dental composite resins reinforced with aluminium borate whisker (ABW).ABW was surface-treated with 0%, 1%, 2%, 3% and 4% silan coupling agent (γ-MPS), and mixed with resin matrix to synthesize 5 groups of composite resins. After heat-cured at 120 degrees centigrade for 1 h, specimens were tested in three-point flexure to measure strength according to ISO-4049. One specimen was selected randomly from each group and observed under scanning electron microscope (SEM). The data was analyzed with SAS 9.2 software package.The flexural strength (117.93±11.9 Mpa) of the group treated with 2% silane coupling agent was the highest, and significantly different from that of the other 4 groups (α=0.01).The amount of silane coupling agent has impact on the flexural strength of dental composite resins reinforced with whiskers; The flexual strength will be reduced whenever the amount is higher or lower than the threshold. Supported by Research Fund of Science and Technology Committee of Shanghai Municipality (08DZ2271100).

To know about Shanghai residents' cognition and approbation to dental practical clinic and discuss the appropriate mode of clinical teaching.100 residents were chosen in five super-markets in five different districts randomly to fill in a questionnaire.475 questionnaires were effective among 502 questionnaires and the efficiency was 94.62%; 10.32% informants knew the practical clinic, 56.84% informants would like to know it; 40.21% informants would like to choose the specialists, meanwhile 37.68% informants would choose the interns; 65.43% informants were satisfied with the dental interns.The cognition and approbation of residents to dental practical clinic should be enhanced, hospitals and schools should strengthen propaganda and improve teaching, in order to advancing the approbation based on upgrading the cognition.

To evaluate the effect of different polishing time on microleakage of different resin composite restorations (Filtek Z350 and Filtek Z250).Standardized preparation (the box-type cavities, 3 mm in length and width,2 mm in depth) was performed at the cemento-enamel junction on buccal surface of 80 premolars.They were randomly divided into two groups,and restored with composite resin(Filtek Z350 or Filtek Z250). According to polishing time, the specimens of each group were randomly classified into four subgroups. They were the subgroups of no polishing, polishing immediately after restoring, polishing after 10 minutes and polishing after 24 hours. All of the restorations were polished with Sof-lex discs. After thermal circulation and soaked for 24 hours in 2% methyl blue solution, the specimens were cut through the midline of the restoration, the microleakage at the occlusal margin and the gingival margin were observed under a stereomicroscope and the leakage depth was measured with Spot version 4.6 software package. The microleakage degree and microleakage depth of different groups were analyzed with SPSS 17.0 software package for Mann -Whitney U test and Kruskal -Wallis test.The microleakage depth of Filtek Z350 groups were(0.655±0.486)mm,(0.626±0.497)mm,(0.648±0.370)mm and (0.639±0.453)mm,respectively. The microleakage depths of Filtek Z250 groups were (0.785±0.553)mm,(0.763±0.491)mm,±0.749±0.608)mm and (0.715±0.588)mm,respectively. No significant differences (P0.05) of microleakage was found among the different groups and subgroups.The polishing system of Sof-lex discs have no significant influence on microleakage of resin composite restorations at different polishing time.

To analyze the patients' trust and satisfaction with dental interns and discuss the countermeasure to improve the trust of patients.One hundred and eighteen patients who came to our clinic were asked to fill in a questionnaire, including the content and trust of patients to dental interns.95.24% of the patients were satisfied with dental interns and 84.13% of them considered that interns were better than they had expected; 51.09% of the patients would like to accept dental interns' diagnose and therapy; 47.83% of the patients were worry about interns medical activities while 28.26% of them did not.Because of various reasons, patients have some inimical emotion to dental interns and unwilling to cooperate with their performance. Interns, clinicians, hospitals and schools should enhance patients' content and trust to upgrade the quality and efficiency of clinical training and practice.

To evaluate the result that students accommodate themselves to the case-based learning(CBL) in the practice of dental trauma.A total of 60 students were randomly assigned to two groups: CBL group and traditional group. Before and after the learning completed, an exam was fulfilled by each student. A questionnaire was fulfilled by CBL group. The questionnaire included the participants' attitudes toward the challenges through CBL and the suggestions for education reform from traditional to CBL method. All statistical analysis was performed using SAS9.0 software package.There was significant difference (P0.01) in score improvement between experimental group and control group of students. Questionnaires survey showed that students generally liked the CBL teaching methods.CBL model can improve not only scores but also the capability of self-study of students. It can also culture the students' good thinking habit in clinic.

To evaluate three kinds of nickel-titanium instruments applied to molar root canal preparation.A total of 90 molars treated by root canal therapy were randomly assigned to three groups: ProTaper group. K3 group and Mtwo group. Crown-down technique was used in ProTaper and K3 group, while Mtwo group was treated with routine preparation technique. All teeth in 3 groups were filled by lateral condensation technique. Treatment effect of 3 groups was evaluated according to pre- and post-operative X-ray films. Wearing and tearing degree of equipment, preparation time and postoperative complications incidence of 3 groups were also compared. All statistical analysis was performed using SAS9.0 software package. Data of root canal curvatures and preparation time was compared using t test; the incidence of complications of root canal treatment and postoperative pain was analyzed using Chi-square test.In three groups, root canals had no deviation or no ledge, could maintain the root canal anatomy in early form, and achieved good preparation results. In this study, four ProTapers, three K3s and one Mtwo instrument were fractured. The average root canal preparation time of the Mtwo group was 3.94 min, significantly less than the ProTaper (4.71 min) group and the K3(4.58 min) group.Three rotary nickel-titanium systems tested in this study are effective in the molar root canal preparation. The Mtwo instrument is much easier and faster during preparation with routine preparation technique.Supported by Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08DZ2271100).

Two new compounds, named ( Z)-7,4′-dimethoxy-6-hydroxyaurone-4- O-β-glucopyranoside (1) and (-)-4- O-(4- O-β-D-glucopyranosylcaffeoyl)quinic acid (2), were isolated from the endophytic fungus Penicillium citrinum of Avicennia marina. Their structures were elucidated on the basis of spectroscopic analysis. Additionally, compound 2 exhibited potent chemoreversal activity, mainly by inhibiting P-glycoprotein efflux pump function.