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The multidrug resistance can be reversed for the decrease of P-gp and LRP by inhibiting PI3K/Akt/NF-κB signal pathway in nasopharynx carcinoma
- Source :
- Bioscience Reports
- Publication Year :
- 2020
- Publisher :
- Portland Press Ltd., 2020.
-
Abstract
- Aim: To investigate the relationship between PI3K/Akt/NF-κB cellular signal pathway and the expression of P-gp and LRP in multidrug resistance (MDR) cell of nasopharyngeal carcinoma. Method: The PI3K, p-Akt and NF-κB/p65 as the activity of PI3K/Akt/NF-κB were detected by Western blot. The expressions of LRP and P-gp were detected by Western blot and real-time PCR. Result: The RIs of CNE/DDP group to DDP, 5-Fu, VCR, ADR and PTX were 35.04, 18.14, 24.13, 12.00 and 10.18, respectively. The RIs of LY-294002 group were 11.77, 5.83, 3.07, 3.86 and 3.34, and PDTC group were 11.08, 6.55, 7.66, 2.18 and 4.05. The expressions of PI3K, p-Akt and NF-κBp65, LRP and P-gp were increased and mRNA of LRP and P-gp were up-regulated in CNE/DDP. The expression of p-Akt in LY-294002 group was down-regulated. The expression of NF-κB p65 in PDTC group was decreased. The mRNA of LRP and P-gp in LY-294002 group and PDTC group were decreased. Conclusion: MDR of nasopharyngeal carcinoma cell can be regulated by activating PI3K/Akt/NF-κB signal pathway and then increase the expression of P-gp and LRP. The MDR of nasopharyngeal carcinoma cell can be reversed by inhibiting PI3K/Akt/NF-κB signal pathway.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B
Pyrrolidines
LRP
Cell
Biophysics
nasopharynx carcinoma
Multidrug resistance
Biochemistry
NF-κB
chemistry.chemical_compound
Phosphatidylinositol 3-Kinases
Western blot
Thiocarbamates
Cell Line, Tumor
Antineoplastic Combined Chemotherapy Protocols
medicine
Humans
Molecular Biology
Protein kinase B
PI3K/AKT/mTOR pathway
Research Articles
Cancer
Phosphoinositide-3 Kinase Inhibitors
Vault Ribonucleoprotein Particles
Messenger RNA
PI3K/Akt
Nasopharyngeal Carcinoma
medicine.diagnostic_test
Chemistry
Transcription Factor RelA
Nasopharyngeal Neoplasms
Cell Biology
medicine.disease
Signaling
Drug Resistance, Multiple
Multiple drug resistance
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Nasopharyngeal carcinoma
Drug Resistance, Neoplasm
Cancer research
P-gp
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 15734935 and 01448463
- Volume :
- 40
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....35b0713dca790907f75c386dc2af14db