1. Lactic acid bacteria isolated from women' breast milk and infants' faeces have appreciable immunogenic and probiotic potentials against diarrheagenic E. coli strains.
- Author
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Obisesan AO, Abiodun OO, and Ayeni FA
- Subjects
- Humans, Animals, Female, Mice, Escherichia coli Infections microbiology, Escherichia coli Infections prevention & control, Escherichia coli Infections veterinary, Infant, RNA, Ribosomal, 16S genetics, Anti-Bacterial Agents pharmacology, Infant, Newborn, Adult, Microbial Sensitivity Tests, Probiotics pharmacology, Feces microbiology, Milk, Human microbiology, Milk, Human immunology, Escherichia coli genetics, Escherichia coli drug effects, Escherichia coli immunology, Lactobacillales isolation & purification, Lactobacillales physiology, Lactobacillales classification, Diarrhea microbiology, Diarrhea prevention & control
- Abstract
Diarrheal diseases remain the leading cause of high mortality among the infants, particularly in the developing countries; Probiotic intervention for diarrhea has been an ongoing novel approach to diarrheal prevention and treatment. This study aims to characterize immunogenic and probiotic properties of lactic acid bacteria (LAB) isolated from human breast milk and neonates' faeces. The LAB isolates from 16 mothers' breast milk and 13 infants' faeces were screened and identified by 16 S rRNA gene partial sequencing. Their antimicrobial activities against 5 strains of diarrheagenic Escherichia coli were tested. Organic acids production was quantified by HPLC, and antibiotic resistance pattern were determined by VITEK
® . Autoaggregation, co-aggregation and hydrophobicity properties were assessed by UV spectrophotometry and immunomodulatory effect was determined in mouse model. Ninety-three LAB of five genera were identified. The most abundant species was Lactiplantibacillus plantarum with inhibition zones ranged from 8.0 to 25.0 ± 1 mm. Lacticaseibacillus rhamnosus A012 had 76.8 mg/mL lactic acid, (the highest concentration), was susceptible to all antibiotics tested. L. plantarum A011 and L. rhamnosus A012 were highly resistance to gastrointestinal conditions. L. rhamnosus A012 produced hydrophobicity of 25.01% (n-hexadecane), 15.4% (xylene) and its autoaggregation was 32.52%. L. rhamnosus A012 and L. plantarum A011 exert immunomodulatory effects on the cyclophosphamide-treated mice by upregulating anti-inflammatory cytokine and downregulating proinflammatory cytokines. Lactobacillus sp. demonstrated good probiotic and immunomodulatory properties. Further works are ongoing on the practical use of the strains., (© 2024. The Author(s).)- Published
- 2024
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