1. Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng
- Author
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Jiang Xu, Fugang Wei, Jun Qian, Zhongjian Chen, Lianjuan Zhang, Ying Gao, He Su, Shilin Chen, Guangfei Wei, Wang Yong, Feng Yang, Zhengwei Jia, and Linlin Dong
- Subjects
0301 basic medicine ,MEP, 2-C-methyl-D-erythritol-4-phosphate ,Metabolite ,MVK, Mevalonate kinase ,Saponin ,FPS, Farnesyl pyrophosphate synthase ,HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase ,DXPS, 1-deoxy-o-xylulose 5-phosphate synthase ,ISPE, 4-(cytidine-5′-diphospho)-2-C-methylerythritol kinase ,DXPR, 1-deoxy-o-xylulose 5-phosphate reductoisomerase ,Transcriptome ,HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase ,chemistry.chemical_compound ,0302 clinical medicine ,lcsh:Botany ,MVD, Mevalonate diphosphate decarboxylase ,ISPH, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase ,chemistry.chemical_classification ,biology ,Chemistry ,HPLC-UV, High-performance liquid chromatography-ultraviolet detection ,musculoskeletal system ,HDS, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphatesynthase ,lcsh:QK1-989 ,IPP, Isoprenyl diphosphate ,Biochemistry ,030220 oncology & carcinogenesis ,Metabolome ,IPPI, Isopentenyl pyrophosphate isomerase ,OPLS-DA, Orthogonal partial least squares-discriminant analysis ,Biotechnology ,Research Article ,MALDI-MS, Matrix-assisted laser desorption/ionization–mass spectrometry ,P450, P450-monooxygenase ,SS, Squalene synthase ,complex mixtures ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Panax plants ,GDPS, Gerenyl diphosphatesynthase ,03 medical and health sciences ,parasitic diseases ,GO, Gene Ontology ,Distribution (pharmacology) ,Saponin distribution ,Panax notoginseng ,Saponin synthesis ,ISPD, 2-C-methylerythritol 4-phosphatecytidyl transferase ,FPP, Farnesyl diphosphate ,PMK, Phosphomevalonate kinase ,DS, Dammarenediol-II synthase ,NCBI Nr, NCBI Non-redundant protein ,biology.organism_classification ,MVA, Mevalonate acid ,MECPS, 2-C-methylerythritol-2,4-cyclophosphate synthase ,UPLC-MS, Ultrahigh-performance liquid chromatography quadrupole time of flight-mass spectrometry ,carbohydrates (lipids) ,WGCNA, Weighted gene coexpression network analysis ,030104 developmental biology ,Complementary and alternative medicine ,Root tissues ,Potential biomarkers ,AACT, Acetoacetyl-CoA acyltransferase ,FDR, False discovery rate ,ORF, Open read frame ,SE, Squalene epoxidase ,UGTs, UDP-glycosyltransferases - Abstract
Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants. Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles. Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P
- Published
- 2019