463 results on '"Guo QM"'
Search Results
2. Purification of the secondary treatment tail water for wastewater reclamation by integrated subsurface-constructed wetlands.
- Author
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A D, Guo QM, Deng YY, Jiang Y, and Chen CX
- Subjects
- Wastewater, Wetlands, Waste Disposal, Fluid methods, Nitrogen analysis, Phosphorus, Water Purification methods, Environmental Pollutants
- Abstract
A whole-year investigation of full-scale integrated subsurface-constructed wetlands (ISCWs) was carried out to purify the tail water from a wastewater treatment plant (WWTP) for wastewater reclamation under four plant species, four hydraulic loading rates (HLRs), and four seasons. The results showed that ISCWs were effective for the purification of WWTP discharge, with the average removal efficiencies of COD, NH
4 + -N, TN, and TP being 48%, 49%, 9%, and 30%, respectively. Typical pollutant concentrations in the treated effluent of ISCWs were 8.19 mg/L COD, 1.76 mg/L NH4 + -N, 11.57 mg/L TN, and 0.36 mg/L TP, which met most of the water quality standards for reusing recycling water. Emergent plants with well-developed root systems may be capable of promoting the decontamination of ISCWs. Seasonal change played an important role in the treatment process: the removal of phosphorus by plant uptake and microbial utilization was more active in the warm season and the co-occurrence of organic degradation and nitrification, whereas the cold season is conducive to exothermic adsorption process of pollutants to substrates. Properly increasing the HLRs may improve the availability of ISCWs according to the requirement of effluent quality. Furthermore, the C/N ratio might be the key factor for the purification effect of ISCWs, because the COD level of WWTP discharge may change the process of NH4 + -N biotransformation.- Published
- 2024
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3. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer.
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Zhao MN, Zhang LF, Sun Z, Qiao LH, Yang T, Ren YZ, Zhang XZ, Wu L, Qian WL, Guo QM, Xu WX, Wang XQ, Wu F, Wang L, Gu Y, Liu MF, and Lou JT
- Subjects
- Animals, Humans, Mice, Cell Line, Tumor, Cell Proliferation, Gene Expression Regulation, Neoplastic, Interleukin-8 metabolism, Mice, Nude, Neoplasm Metastasis, Carcinoma, Non-Small-Cell Lung pathology, Lung Neoplasms pathology, MicroRNAs metabolism
- Abstract
Bone metastasis is one of the main complications of lung cancer and most important factors that lead to poor life quality and low survival rate in lung cancer patients. However, the regulatory mechanisms underlying lung cancer bone metastasis are still poor understood. Here, we report that microRNA-182 (miR-182) plays a critical role in regulating osteoclastic metastasis of lung cancer cells. We found that miR-182 was significantly upregulated in both bone-metastatic human non-small cell lung cancer (NSCLC) cell line and tumor specimens. We further demonstrated that miR-182 markedly enhanced the ability of NSCLC cells for osteolytic bone metastasis in nude mice. Mechanistically, miR-182 promotes NSCLC cells to secrete Interleukin-8 (IL-8) and in turn facilitates osteoclastogenesis via activating STAT3 signaling in osteoclast progenitor cells. Importantly, systemically delivered IL-8 neutralizing antibody inhibits NSCLC bone metastasis in nude mice. Collectively, our findings identify the miR-182/IL-8/STAT3 axis as a key regulatory pathway in controlling lung cancer cell-induced osteolytic bone metastasis and suggest a promising therapeutic strategy that targets this regulatory axis to interrupt lung cancer bone metastasis., (© 2023. The Author(s).)
- Published
- 2023
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4. A three-year study on the treatment of domestic-industrial mixed wastewater using a full-scale hybrid constructed wetland.
- Author
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A D, Deng YY, Guo QM, Jiang Y, and Chen CX
- Subjects
- Wetlands, Nitrogen analysis, Oxygen, Denitrification, Wastewater, Waste Disposal, Fluid
- Abstract
Three full-scale constructed wetlands (CWs), namely vertical flow (VFCW), surface flow (SFCW), and horizontal flow (HFCW) systems, were combined in a series process to form a hybrid CW, which was used for the treatment performance of domestic-industrial mixed wastewater and investigated over a three-year period. The hybrid CW demonstrated that it is effective and stable during the long-term treatment of high-loading mixed wastewater under different operation years, season changes, and technology processes, with the average removal efficiencies of suspended solids, chemical oxygen demand, biological oxygen demand, total nitrogen, ammonia nitrogen, nitrate nitrogen, and total phosphorous being 84, 40, 54, 54, 70, 40, and 46%, respectively. The effluent quality of the hybrid CW reached the highest discharge standard for wastewater treatment plants. First, a variety of pollutants from the mixed wastewater were effectively removed in the subsurface processes (VFCW and HFCW) via substrate adsorption and degradation of the attached biofilm. The higher dissolved oxygen content and oxygen transfer capacity values in the VFCW were favourable for the occurrence of aerobic pathways (such as nitrification and inorganic phosphorus oxidation). In addition, with the large consumption of oxygen in the previous process, the oxygen-enriching capacity of the SFCW processes, provided aerobic potential for the next stage. In particular, the plant debris in the SFCW temporarily increased the organics and suspended solids, further increasing the C/N ratio, which was beneficial for denitrification as the main nitrogen removal pathway in the HFCW., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2023
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5. Botany, traditional uses, phytochemistry and pharmacological activity of Crataegus pinnatifida (Chinese hawthorn): a review.
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Zhang SY, Sun XL, Yang XL, Shi PL, Xu LC, and Guo QM
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- Molecular Docking Simulation, Flavonoids chemistry, Fruit chemistry, Medicine, Chinese Traditional, Crataegus chemistry, Botany
- Abstract
Objectives: Crataegus pinnatifida (C. pinnatifida), including C. pinnatifida Bge. and its variant C. pinnatifida Bge. var. major N, E. Br., has traditionally been used as a homologous plant for traditional medicine and food in ethnic medical systems in China. Crataegus pinnatifida, especially its fruit, has been used for more than 2000 years to treat indigestion, stagnation of meat, hyperlipidemia, blood stasis, heart tingling, sores, etc. This review aimed to provide a systematic summary on the botany, traditional uses, phytochemistry, pharmacology and clinical applications of C. pinnatifida., Key Findings: This plant contains flavonoids, phenylpropanoids, terpenoids, organic acids, saccharides and essential oils. Experimental studies showed that it has hypolipidemic, antimyocardial, anti-ischemia, antithrombotic, anti-atherosclerotic, anti-inflammatory, antineoplastic neuroprotective activity, etc. Importantly, it has good effects in treating diseases of the digestive system and cardiovascular and cerebrovascular systems., Summary: There is convincing evidence from both in vitro and in vivo studies supporting the traditional uses of C. pinnatifida. However, multitarget network pharmacology and molecular docking technology should be used to study the interaction between the active ingredients and targets of C. pinnatifida. Furthermore, exploring the synergy of C. pinnatifida with other Chinese medicines to provide new understanding of complex diseases may be a promising strategy., (© The Author(s) 2022. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2022
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6. Inflammation-Related Gene Signature: An Individualized Risk Prediction Model for Kidney Renal Clear Cell Carcinoma.
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Zhang Z, Wei YY, Guo QM, Zhou CH, Li N, Wu JF, Li YT, Gao WW, and Li HL
- Abstract
Background: There is much evidence that confirms the inextricable link between inflammation and malignancy. Inflammation-related regulators were involved in the progression of kidney renal clear cell carcinoma (KIRC). However, the predictive role of single gene biomarkers is inadequate, and more accurate prognostic models are necessary. We undertook the current research to construct a robust inflammation-related gene signature that could stratify patients with KIRC., Methods: The transcriptome sequencing data along with clinicopathologic information of KIRC were obtained from TCGA. A list of inflammation-related genes was acquired from the Molecular Signatures Database. Using the RNA-seq and survival time data from the TCGA training cohort, an inflammation-related gene signature was built using bioinformatic methods, and its performance in predicting patient prognosis was assessed by Kaplan-Meier and ROC curve analyses. Furthermore, we explored the association of risk score with immune score, stromal score, tumor immune-infiltrating cells (TIICs), immunosuppressive molecules, m6A regulators, and autophagy-related biomarkers., Results: Herein, nine inflammation-related hub genes (ROS1, PLAUR, ACVR2A, KLF6, GABBR1, APLNR, SPHK1, PDPN, and ADORA2B) were determined and used to build a predictive model. All sets, including training set, four testing sets, and the entire TCGA group, were divided into two groups (low and high risk), and Kaplan-Meier curves all showed an adverse prognosis for patients in the high-risk group. ESTIMATE algorithm revealed a higher immune score in the high-risk subgroup. CIBERSORT algorithm illustrated that the high-risk group showed higher-level immune infiltrates. Furthermore, LAG3, TIGIT, and CTLA4 were overexpressed in the high-risk subgroup and positively associated with risk scores. Moreover, except for METTL3 and ALKBH5, the other m6A regulators decreased in the high-risk subgroup., Conclusions: In conclusion, a novel inflammation-related gene signature comprehensively constructed in the current study may help stratify patients with KIRC., Competing Interests: The authors declare that they have no conflicts of interest regarding the publication of this paper., (Copyright © 2022 Ze Zhang et al.)
- Published
- 2022
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7. EpCAM expression in esophageal cancer and its correlation with immunotherapy of solitomab.
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Yu L, Guo QM, Wang Y, Xu Y, Liu L, and Zhang XT
- Abstract
Background: Recurrence of esophageal cancer (EC) after chemotherapy may mainly be explained by the existence of chemotherapy-resistant cells, and an effective drug against chemotherapy-resistant cells is highly sought. The aim of this study was to investigate the cytotoxicity of bispecific antibody solitomab combined with γ δ T cells on Eca109 cell spheres., Methods: We cultured Eca109 cell spheres in serum-free medium, and the morphological differences between wild-type Eca109 cells and Eca109 cell spheres were compared by microscope and flow cytometry. Different concentrations of nanoparticle albumin-bound paclitaxel (Nab-PTX) and cisplatin were used to treat the two groups of cells and compare their drug resistance. Flow cytometry was then used to detect the expression level of epithelial cell adhesion molecule (EpCAM) and the cytotoxicity of γ δ T cells combined with bispecific antibody solitomab on the two groups., Results: Flow cytometry analysis showed that Eca109 cell spheres were smaller in size and had less cytoplasmic granules and CCK-8 assay showed that the viability of Eca109 cell spheres treated with different concentrations of Nab-PTX and cisplatin was significantly higher than that of wild-type Eca109 cells (P<0.05). Flow cytometry also showed that the expression level of EpCAM on Eca109 cell spheres was higher than that of wild-type Eca109 cells. Co-culture experiment showed that there was no significant difference in the cytotoxicity of γ δ T cells to wild-type Eca109 cells and Eca109 cell spheres without solitomab. However, after adding solitomab, the cytotoxicity of γ δ T cells to Eca109 cell spheres was significantly higher than that of wild-type Eca109 cells (P<0.05)., Conclusions: EC Eca109 cell spheres have strong stem cell characteristics such as multidrug resistance and may contain a high proportion of EC stem cells. Further, EC Eca109 cell spheres have a high expression level of EpCAM, and EpCAM may be one of the markers of EC stem cells. Therefore, EpCAM could be used as a potential molecular target of immunotherapy for EC, and solitomab may become an effective immunotherapeutic drug for chemotherapy-resistant EC cells., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/jtd-21-442). The authors have no conflicts of interest to declare., (2021 Journal of Thoracic Disease. All rights reserved.)
- Published
- 2021
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8. [The Role of Virtual Reality Technology in Medical Education in the Context of Emerging Medical Discipline].
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Gao Y, Zhao QP, Zhou XD, Guo QM, and Xi T
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- China, Clinical Competence, Technology, Education, Medical, Virtual Reality
- Abstract
According to Healthy China, a national strategy of the Government of China, new requirements were put forward for high-quality medical education, high-level surgical research, and precise clinical diagnosis and treatment. In the context of Emerging Medical Discipline, a strategic blueprint of medical education in China, this paper reviews the concept and core value of virtual reality (VR) and its significant role in the medical industry. On that basis, we explore the role of VR technology in medical training against the background of Emerging Medicine Discipline. Furthermore, typical cases are presented to help analyze and illustrate in detail the important role of VR technology in the teaching and training of stomatological and clinical procedures, skills assessment, online self-directed training, and clinical thinking skills training. We herein summarize useful information from past experience so as to help build innovative models of medical education in the context of Emerging Medical Discipline., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).)
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- 2021
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9. In vivoexpression of Toll-like receptor 2, Toll-like receptor 4, CSF2 and LY64 in Chinese chronic periodontitis patients
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Ming-zhu Zhang, Rong Shu, Guo Qm, Dengtang Liu, and Yang Sun
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Adult ,Male ,China ,medicine.medical_treatment ,Gingiva ,Biology ,Pathogenesis ,Immune system ,Antigens, CD ,Reference Values ,medicine ,Humans ,RNA, Messenger ,Receptor ,General Dentistry ,Aged ,Toll-like receptor ,Granulocyte-Macrophage Colony-Stimulating Factor ,Gingival Crevicular Fluid ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Chronic periodontitis ,Toll-Like Receptor 2 ,Toll-Like Receptor 4 ,TLR2 ,Cytokine ,Otorhinolaryngology ,Case-Control Studies ,Chronic Periodontitis ,Immunology ,TLR4 ,Female ,Periodontal Index - Abstract
Oral Diseases (2010) 16, 343–350 Objective: Toll-like receptors (TLRs) are the essential components in the innate and adaptive immune systems. Colony stimulating factor 2 (CSF2) is a cytokine that may prevent endotoxin tolerance, and LY64 has the ability to interfere with the recognition of bacteria via TLR4. The aim of this study was to explore the in vivo expressions of TLR2, TLR4, CSF2 and LY64 in Chinese chronic periodontitis patients. Methods: Gingival biopsies were collected from 24 chronic periodontitis patients and 19 healthy controls. The gene expression profiles of TLR2, TLR4, CSF2 and LY64 were investigated by real-time polymerase chain reaction, and the protein expressions of TLR2 and TLR4 were detected by immunohistochemistry. In addition, the levels of CSF2 in gingival crevicular fluid (GCF) were determined by ELISA. Results: The higher mRNA expressions of TLR2, TLR4 and CSF2, and the lower mRNA expression of LY64 were detected in chronic periodontitis patients. And the increased protein expressions of TLR2 and TLR4 were confirmed by immunohistochemistry. In addition, the increase of total amount of CSF2 in GCF was observed in chronic periodontitis patients. Conclusions: Our results suggest that TLR2 and TLR4 may play a role in periodontal pathogenesis. In addition, CSF2 and LY64 may contribute to the regulation of inflammatory response and maintaining periodontal homeostasis.
- Published
- 2010
10. Detection of Plasma EGFR Mutations in NSCLC Patients with a Validated ddPCR Lung cfDNA Assay.
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Guo QM, Wang L, Yu WJ, Qiao LH, Zhao MN, Hu XM, Sun YM, Ni S, Xu YH, and Lou JT
- Abstract
Purpose : The clinical utility of cell-free DNA (cfDNA) to assess EGFR mutations is increasing. However, there are limited studies determining their clinical validity and utility. The value of cfDNA assays in cancer management remains controversial. Methods : In this study, we first evaluated the analytical performance of the ddPCR Lung cfDNA Assay. We next analyzed the concordance of the results with tissue amplification refractory mutation system PCR (ARMS-PCR) and plasma next-generation sequencing (NGS) genotyping. Finally, we assessed its clinical utility by exploring the association of cfDNA EGFR mutations with metastatic sites and the efficacy of EGFR-TKIs treatment. Results : The ddPCR Lung cfDNA Assay demonstrated a limit of blank of 1 droplet per reaction, an analytical specificity of 100%, and detection limit of 0.05%, 0.05%, and 0.1% for E746_A750del , L858R , and T790M , respectively. With tissue ARMS-PCR as a standard for comparison, the clinical sensitivity and specificity of ddPCR were 62.5% (15/24) and 100% (82/82) for E746_A750del , and 75.0% (15/20) and 94.2% (81/86) for L858R , respectively. The ddPCR showed high concordance with NGS in determining cfDNA EGFR mutations. Patients with bone and/or brain metastasis showed a higher detection rate and mutant abundance of cfDNA EGFR mutations compared to those with other sites of metastasis. Moreover, EGFR-TKIs treatment was effective in patients with sensitive EGFR mutations in either plasma cfDNA or tumor tissue-derived DNA. Conclusions : We validated in this study that the ddPCR Lung cfDNA Assay is reliable for detection of EGFR mutations in lung cancers, in terms of analytical performance, clinical validity and utility., Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
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- 2019
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11. CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.
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Zhou KX, Xie LH, Peng X, Guo QM, Wu QY, Wang WH, Zhang GL, Wu JF, Zhang GJ, and Du CW
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- Animals, Apoptosis drug effects, Apoptosis radiation effects, Benzylamines, Cell Cycle drug effects, Cell Cycle radiation effects, Cell Line, Tumor, Cell Survival drug effects, Cell Survival radiation effects, Chemoradiotherapy, Cyclams, Female, Humans, Mice, Nude, Receptors, CXCR4 metabolism, Triple Negative Breast Neoplasms metabolism, Triple Negative Breast Neoplasms pathology, Heterocyclic Compounds pharmacology, Radiation, Ionizing, Receptors, CXCR4 antagonists & inhibitors, Triple Negative Breast Neoplasms therapy, Xenograft Model Antitumor Assays methods
- Abstract
Radiation therapy (RT) is one of the primary modalities for triple-negative breast cancer (TNBC) treatment. However, due to the pro-metastatic potential of radiation and the intrinsic radiation resistance of some tumors, many patients experience RT failure, which leads to cancer relapse and distant metastasis. This preclinical study evaluated the efficacy of the antagonist of the SDF-1 receptor CXCR4, AMD3100, as a radiosensitizer in TNBC models. The combined effect of ionizing radiation and AMD3100 was determined in vitro by surviving fraction, cell cycle distribution, Bax and Bcl-2 expression, and apoptosis assays in a TNBC cell line (MDA-MB-231). For in vivo studies, human xenograft athymic nude mice were used. Treatment of TNBC cells with AMD3100 significantly augmented cellular radiosensitivity. Radiosensitivity was enhanced specifically through increased Bax expression, reduced Bcl-2 expression, prolonged G2-M arrest, and increased apoptosis. Combined treatment with AMD3100 and irradiation also enhanced tumor growth delay, with an enhancement factor ranging from 1.5 to 1.8. These findings support the evaluation of antagonists of the SDF-1 receptor CXCR4, such as AMD3100, as potent radiosensitizers in TNBC., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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12. Identification of the novel allele, HLA-B*15:388, in a Chinese bone marrow donor.
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Han B, Zhu YL, Guo QM, Pang ST, and Feng ZH
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- Base Sequence, Histocompatibility Testing, Humans, Alleles, Asian People genetics, Bone Marrow metabolism, HLA-B Antigens genetics, Tissue Donors
- Abstract
The novel allele, HLA-B*15:388, was identified in a Chinese bone marrow donor by sequence-based typing., (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2018
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13. [Survey of periodontal health in medical college students after 3 years of periodontal health maintenance].
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Ge LH, Lin ZK, Guo QM, Ni J, Qian JL, and Shu R
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- Adolescent, Follow-Up Studies, Gingival Hemorrhage, Humans, Oral Health, Periodontal Index, Periodontal Pocket, Students, Students, Medical, Surveys and Questionnaires, Dental Plaque Index, Periodontal Attachment Loss, Periodontal Diseases prevention & control
- Abstract
Purpose: To observe and evaluate the status of periodontal disease in young people and the effect of intervention to control the development of periodontal diseases., Methods: One hundred and fifty-three medical college students were randomly divided into group A (receiving interventions) and group B (no interventions). They were followed up for 3 years. The subjects in group A received oral health education, including selection of the toothbrush, the right way to brush teeth, the use of dental floss and interdental brush. At the same time ,they were given initial periodontal treatment according to the actual situation, and received oral health education, periodontal maintenance treatment, and reinforced plaque control every six months. The changes of debris index (DI), calculus index (CI), probing depth (PD), clinical attachment loss (CAL), bleeding on probing (BOP) and gingival index (GI) before and after interventions were compared. Statistical analysis was performed using SAS 6.12 software package., Results: Three years later, CI and DI in group A declined significantly compared to the baseline (P<0.01), but there was no significant changes in group B (P>0.05). There was significant difference in the changes of PD, BOP and GI between group A and B (P<0.01). Significant difference of the change of CAL between group A and B was also found(P<0.05), CAL in group B was significantly higher than that in group A., Conclusions: There are positive effects of regular periodontal health maintenance and oral health education on periodontal health.
- Published
- 2017
14. [Preparation of chaperone-antigen peptide vaccine derived from human gastric cancer stem cells and its immune function].
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Jiang YQ, Guo QM, Xu XP, Liang JC, He YY, An SH, Su F, Li CY, and Huang CX
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- Cancer Vaccines immunology, Cell Proliferation, Cytotoxicity Tests, Immunologic, HSP70 Heat-Shock Proteins immunology, HSP70 Heat-Shock Proteins isolation & purification, HSP90 Heat-Shock Proteins immunology, HSP90 Heat-Shock Proteins isolation & purification, Heat-Shock Proteins isolation & purification, Humans, Lymphocyte Activation immunology, Heat-Shock Proteins immunology, Neoplastic Stem Cells immunology, Peptides immunology, Stomach Neoplasms pathology
- Abstract
Objective: To explore the method of extracting chaperone antigen peptide complexes from gastric cancer stem cells and its immune function. Methods: Gastric cancer stem cells and gastric cancer cells were screened by low temperature ultrasonic lysis. After salting out and dialysis, the lysate supernatant was processed with SDS-PAGE to analyze the expression of chaperone antigen peptide complexes, and then was separated and purified with CNBr-activated SepharoseTM 4B. Reverse high pressure liquid chromatography (HPLC), SDS-PAGE and Western blotting were used to analyze the purity and nature of the acquired albumen. Lymphocyte proliferation assay and lymphocytotoxicity assay were used to ditermine the immunological activity of the chaperone-antigen peptide complexes. Results: The chaperone antigen peptide complexes of gastric cancer stem cells were prepared and identified successfully, of which the main components were the antigen peptides of HSP60, HSP70, HSP90 and HSP110. 0.75 μg and 1.00 μg HSP70-antigen peptide and 1.00 μg HSP90-antigen peptide activated lymphocytes significantly. Their A (490) values were 0.26±0.03, 0.45±0.05 and 0.32±0.04, respectively, while the corresponding doses of HSP60-antigen peptide and HSP110-antigen peptide did not activate lymphocytes. The killing rates of 1.00 μg HSP70-antigen peptide and 1.00 μg HSP70 were (45.0±2.0)% and (16.0±2.0)%, respectively, showing a significant difference ( P =0.012). Similarly, the killing rates of 1.00 μg HSP90-antigen peptide and 1.00 μg HSP90 were (36.0±5.0)% and (13.0±4.0)%, respectively, also showing a significant difference ( P =0.048). Conclusions: The amount of chaperone antigen peptide complexes in gastric cancer cells is extremely low, but it is obviously increased in gastric cancer stem cells. After purification, the chaperone antigen peptide complexes with high purity can be prepared. The extracted chaperone antigen peptide complexes have stronger immunogenicity, and can be used to make tumor vaccine in vitro, which may have a good application value in the targeted therapy of gastric cancer.
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- 2017
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15. Reversal of cisplatin resistance in non-small cell lung cancer stem cells by Taxus chinensis var.
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Jiang YQ, Xu XP, Guo QM, Xu XC, Liu QY, An SH, Xu JL, Su F, and Tai JB
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- ATP Binding Cassette Transporter, Subfamily B antagonists & inhibitors, ATP Binding Cassette Transporter, Subfamily B genetics, ATP Binding Cassette Transporter, Subfamily B metabolism, Antineoplastic Agents chemistry, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung metabolism, Carcinoma, Non-Small-Cell Lung pathology, Cell Line, Tumor, Cisplatin pharmacology, Drug Combinations, Drug Resistance, Neoplasm genetics, Drugs, Chinese Herbal, Humans, Lung Neoplasms drug therapy, Lung Neoplasms genetics, Lung Neoplasms metabolism, Lung Neoplasms pathology, Multidrug Resistance-Associated Proteins antagonists & inhibitors, Multidrug Resistance-Associated Proteins genetics, Multidrug Resistance-Associated Proteins metabolism, Neoplastic Stem Cells metabolism, Neoplastic Stem Cells pathology, Plant Extracts chemistry, Rhodamine 123 metabolism, Signal Transduction, Vault Ribonucleoprotein Particles antagonists & inhibitors, Vault Ribonucleoprotein Particles genetics, Vault Ribonucleoprotein Particles metabolism, Antineoplastic Agents pharmacology, Drug Resistance, Neoplasm drug effects, Gene Expression Regulation, Neoplastic, Neoplastic Stem Cells drug effects, Plant Extracts pharmacology, Taxus chemistry
- Abstract
Drug resistance in cells is a major impedance to successful treatment of lung cancer. Taxus chinensis var. inhibits the growth of tumor cells and promotes the synthesis of interleukins 1 and 2 and tumor necrosis factor, enhancing immune function. In this study, T. chinensis var.-induced cell death was analyzed in lung cancer cells (H460) enriched for stem cell growth in a defined serum-free medium. Taxus-treated stem cells were also analyzed for Rhodamine 123 (Rh-123) expression by flow cytometry, and used as a standard functional indicator of MDR. The molecular basis of T. chinensis var.-mediated drug resistance was established by real-time PCR analysis of ABCC1, ABCB1, and lung resistance-related protein (LRP) mRNA, and western blot analysis of MRP1, MDR1, and LRP. Our results revealed that stem cells treated with higher doses of T. chinensis var. showed significantly lower growth inhibition rates than did H460 cells (P < 0.05). The growth of stem and H460 cells treated with a combination of T. chinensis var. and cisplatin was also significantly inhibited (P < 0.05). Rh-123 was significantly accumulated in the intracellular region and showed delayed efflux in stem cells treated with T. chinensis var. (P < 0.05), compared to those treated with verapamil. T. chinensis var.-treated stem cells showed significant downregulation of the ABCC1, ABCB1, and LRP mRNA and MRP1, MDR1, and LRP (P < 0.05) compared to H460 cells. Thus, T. chinensis var.-mediated downregulation of MRP1, MDR1, and LRP might contribute to the reversal of drug resistance in non-small cell lung cancer stem cells., Competing Interests: The authors declare no conflict of interest.
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- 2016
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16. [Study on Molecular Biology of Rare Two A307 Phenotypes].
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Han B, Feng ZH, Guo QM, and Wang MM
- Subjects
- Alleles, DNA Mutational Analysis, Exons, Genotype, Humans, Mutation, Polymerase Chain Reaction, ABO Blood-Group System genetics, Phenotype
- Abstract
Objective: To identify the genotypes of the 2 blood samples whose serological typing were difficult by DNA sequencing analysis, and to investigate the molecular genetic basis of their genotypes., Methods: The 2 blood samples were preliminary genotyped by PCR-SSP. The complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and clonal sequenced in order to identify the genotypes., Results: The forward typing showed that both samples were weak A, while the reverse typing showed that the samples contained anti-A1. They were preliminarily genotyped as A/O1., Results: The sequencing analysis showed that the 2 samples contained the nt467C>T and nt745C>T mutation in the A allele, which resulted in an amino acid change from Proline (Pro) to Leucine (Leu) at codon 156 and also from Arginine (Arg) to Tryptophan (Trp) at codon 249., Conclusion: Through serology results and sequencing analysis, the 2 samples are identified as rare A307 phenotypes.
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- 2016
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17. Commentary: systems biology and its relevance to alcohol research.
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Guo QM and Zakhari S
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- 2008
18. DNA microarray and cancer.
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Guo QM and Guo, Qingbin M
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- 2003
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19. [Preparation of hydrophilic matrix sustained release tablets of total lactones from Andrographis paniculata and study on its in vitro release mechanism].
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Xu FF, Shi W, Zhang H, Guo QM, Wang Zhen-Zhong, Bi YA, Wang ZM, and Xiao W
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- Hydrophobic and Hydrophilic Interactions, Kinetics, Tablets chemistry, Andrographis chemistry, Delayed-Action Preparations chemistry, Drug Compounding methods, Drugs, Chinese Herbal chemistry, Lactones chemistry
- Abstract
In this study, hydrophilic matrix sustained release tablets of total lactones from Andrographis paniculata were prepared and the in vitro release behavior were also evaluated. The optimal prescription was achieved by studying the main factor of the type and amount of hydroxypropyl methylcellulose (HPMC) using single factor test and evaluating through cumulative release of three lactones. No burst drug release from the obtained matrix tablets was observed. Drug release sustained to 14 h. The release mechanism of three lactones from A. paniculata was accessed by zero-order, first-order, Higuchi and Peppas equation. The release behavior of total lactones from A. paniculata was better agreed with Higuchi model and the drug release from the tablets was controlled by degradation of the matrix. The preparation of hydrophilic matrix sustained release tablets of total lactones from A. paniculata with good performance of drug release was simple.
- Published
- 2015
20. miRNA-146 negatively regulates the production of pro-inflammatory cytokines via NF-κB signalling in human gingival fibroblasts.
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Xie YF, Shu R, Jiang SY, Song ZC, Guo QM, Dong JC, and Lin ZK
- Abstract
Objective: In human gingival fibroblasts (HGFs), TLR4 recognises Pathogen-associated molecular patterns (PAMPs), such as LPS, and subsequently activates downstream signals that lead to the production of pro-inflammatory cytokines. The aim of this study was to explore the mechanisms of LPS-induced miRNA-146 regulation of TLR4 signals in HGFs., Materials and Methods: HGFs were treated with Porphyromonas gingivalis (P.g) LPS, the cells were harvested, and kinase phosphorylation levels were detected by western blot. Selective pharmacological inhibitors and agonists were used to block or activate the relevant kinases, miRNA-146a/b expression levels were detected by real-time PCR, and IL-1, IL-6, and TNF-α production were measured by enzyme-linked immunosorbent assays (ELISA). A luciferase reporter plasmid containing miRNA-146a/b promoter was tested in terms of p50/p65 regulation., Results: After P.g LPS treatment, NF-κB and Erk1/2 were strongly activated in HGFs. miRNA-146a/b, IL-1, IL-6 and TNF-α levels were down-regulated when NF-κB inhibitor was used. p50/p65 strongly activated miRNA-146a/b promoter as measured with the luciferase assay., Conclusion: In TLR4 signalling in HGFs, both miRNA-146a and miRNA-146b are downstream targets of NF-κB, but not of AP-1 signalling. miRNA-146a/b expression was specifically dependent on NF-κB but not Erk1/2 or JNK signalling.
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- 2014
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21. [Cloning, prokaryotic expression, and functional identification of a sesquiterpene synthase gene (AsSS4) from Aquilaria sinensis].
- Author
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Liang L, Guo QM, Zhang Z, Xu YH, Han XM, and Liu J
- Subjects
- Alkyl and Aryl Transferases genetics, Azulenes, Cloning, Molecular, DNA, Complementary, Escherichia coli, Monocyclic Sesquiterpenes, Open Reading Frames, Polyisoprenyl Phosphates, Recombinant Proteins biosynthesis, Sesquiterpenes metabolism, Sesquiterpenes, Guaiane, Thymelaeaceae genetics, Alkyl and Aryl Transferases biosynthesis, Thymelaeaceae enzymology
- Abstract
A sesquiterpene synthase (AsSS4) full-length open reading frame (ORF) cDNA was cloned from wounded stems of Aquilaria sinensis by RT-PCR method. The result showed that the ORF of AsSS4 was 1,698 bp encoding 565 amino acids. Prokaryotic expression vector pET28a-AsSS4 was constructed and transformed into E. coli BL21 (DE3) pLysS. Recombinant AsSS4 protein was obtained after induction by IPTG and SDS-PAGE analysis with a MW of 64 kD. Enzymatic reactions using farnesyl pyrophosphate showed that recombinant AsSS4 protein purified by Ni-agarose gel yielded five sesquiterpene compounds, cyclohexane, 1-ethenyl-1-methyl-2, 4-bis(1-methylethenyl)-, β-elemene, α-guaiene, α-caryophyllene and δ-guaiene. This paper reported the first cloning and functional characterization of AsSS4 gene from A. sinensis, which will establish a foundation for future studies on the molecular mechanisms of wound-induce agarwood formation in A. sinensis
- Published
- 2014
22. [Effects of fenofibrate on the growth and migration of ovarian cancer cells in vitro].
- Author
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Wang H, He CH, Bai LP, Guo QM, and Zheng A
- Subjects
- Apoptosis drug effects, Cell Line, Tumor drug effects, Cell Movement drug effects, Culture Media, Serum-Free, Female, Humans, Cell Proliferation drug effects, Fenofibrate pharmacology, Ovarian Neoplasms pathology
- Abstract
Objective: To investigate the effects of fenofibrate, a lipid-lowering drug, on the growth and migration of human ovarian cancer cells SKOV3 in vitro., Methods: A human ovarian cancer cell line (SKOV3) as the research object, was incubated with serum-free media for 24 h. These cells were then treated by appropriate concentrations of fenofibrate for different time, including control and experimental groups. Cell proliferation was evaluated by MTT assay. Apoptosis was detected by Hoechst/PI and Annexin-V/PI fluorescent assay. The migration of cells was measured by the scratch-wound healing assay., Results: The MTT assay results demonstrated that the fenofibrate (10, 25, 50, 75, 100 micromol/L) could inhibit the proliferation of SKOV3 cells after 24, 48 and 72 h treatment (P < 0.05). The inhibition rate for 24, 48, 72 h-treatment was 55.72% +/- 0.28%, 57.63% +/- 0.47%, 72.41% +/- 0.62% respectively (P < 0.05). The effects increased with the concentrations. Hoechst/PI and Annexin-V/PI fluorescent assay showed that after stimulus for 24 h, fenofibrate induced apoptosis of SKOV3 cells in a concentration-dependent manner was observed. A significant inhibited cells migration distance (P < 0.05) evaluated with scratch-wound healing assay was observed after treatment with fenofibrate (10, 25, 50, 75, 100 micromol/L) for 24 h., Conclusion: Lipid-lowering drug fenofibrate can inhibit the growth and migration of human ovarian cancer cell SKOV3 in vitro, to some extent induce apoptosis. But the detailed mechanism need to be further studied.
- Published
- 2014
23. [Study on extracting and separating curcuminoids from Curcuma longa rhizome using ultrasound strengthen by microemulsion].
- Author
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Yue CH, Zheng LT, Guo QM, and Li KP
- Subjects
- Curcumin chemistry, Drugs, Chinese Herbal chemistry, Emulsifying Agents chemistry, Plant Oils chemistry, Rhizome chemistry, Surface-Active Agents chemistry, Technology, Pharmaceutical methods, Curcuma chemistry, Curcumin analogs & derivatives, Curcumin isolation & purification, Emulsions chemistry, Ultrasonics
- Abstract
Objective: To establish a new method for the extraction and separation of curcuminoids from Curcuma longa rhizome by cloud-point preconcentration using microemulsions as solvent., Methods: The spectrophotometry was used to detect the solubility of curcumin in different oil phase, emulsifier and auxiliary emulsifier, and the microemulsion prescription was used for false three-phase figure optimization. The extraction process was optimized by uniform experiment design. The curcuminoids were separated from microemulsion extract by cloud-point preconcentration., Results: Oil phase was oleic acid ethyl ester; Emulsifier was OP emulsifier; Auxiliary emulsifier was polyethylene glycol(peg) 400; The quantity of emulsifier to auxiliary emulsifier was the ratio of 5: 1; Microemulsion prescription was water-oleic acid ethyl ester-mixed emulsifier (0.45:0.1:0.45). The optimum extraction process was: time for 12.5 min, temperature of 52 degrees C, power of 360 W, frequency of 400 kHz, and the liquid-solid ratio of 40:1. The extraction rate of curcuminoids was 92.17% and 86.85% in microemulsion and oil phase, respectively., Conclusion: Curcuminoids is soluble in this microemulsion prescription with good extraction rate. This method is simple and suitable for curcuminoids extraction from Curcuma longa rhizome.
- Published
- 2014
24. [Cloning and expression analysis of cinnamate 4-hydroxylase (C4H) reductase gene from Aquilaria sinensis].
- Author
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Liang L, Han XM, Zhang Z, Guo QM, Xu YH, Liu J, and Liao YC
- Subjects
- Amino Acid Sequence, Models, Molecular, Molecular Sequence Data, Open Reading Frames, Oxidoreductases chemistry, Oxidoreductases metabolism, Phylogeny, Plant Proteins chemistry, Plant Proteins metabolism, Thymelaeaceae chemistry, Thymelaeaceae genetics, Trans-Cinnamate 4-Monooxygenase chemistry, Trans-Cinnamate 4-Monooxygenase metabolism, Cloning, Molecular, Oxidoreductases genetics, Plant Proteins genetics, Thymelaeaceae enzymology, Trans-Cinnamate 4-Monooxygenase genetics
- Abstract
The study aimed to clone the open reading frame of cinnamate 4-hydroxylase (C4H) from Aquilaria sinensis and analyze the bioinformatics and expression of the gene. One unique sequence containing C4H domain was discovered in our previous reported wound transcriptome dataset of A. sinensis. The open reading frame of C4H was cloned by RT-PCR strategy with the template of mixed RNA extracted from A. sinensis stem which treated by different wound time. The bioinformatic analysis of this gene and its corresponding protein was performed. C4H expression profiles in responds to MeJA (methyl jasmonate) application were analyzed by real-time PCR. The length of C4H open reading frame (ORF) was 1 515 bp, encoding 514 amino acids. The GenBank accession number is KF134783. Inducible-experiments showed that the genes were induced by mechanical wound as well as MeJA induction, and reached the highest expression level at 8 h and 20 h, respectively. The full-length cDNA of C4H and its expression patterns will provide a foundation for further research on its function in the molecular mechanisms of aromatic compounds and flavonoids biosynthesis.
- Published
- 2014
25. [Effects of rhAm on attachment, proliferation and immigration of human fibroblasts].
- Author
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Lin ZK, Shu R, Cheng L, Song ZC, Guo QM, and Zhang XL
- Subjects
- Animals, Cell Adhesion, Humans, Swine, Cell Movement, Cells, Cultured, Fibroblasts, Periodontal Ligament
- Abstract
Purpose: To compare the effects of 25 kDa full-length rhAm and porcine EMPs on cell behaviors of human periodontal ligament fibroblasts (HPDLF) and foreskin fibroblasts(HFF)., Methods: rhAm was induced by BL21/pET28a-His-SUMO-rhAm express system, and 25 kDa full-length rhAm was analyzed by SDS-PAGE and Western blot. EMPs were extracted by acetic acid method. HPDLF and HFF were cultured in vitro. The cells were treated with rhAm and EMPs at different concentrations. The cell adhesion, proliferation and migration assays were qualitatively analyzed. The data was statistically analyzed with SAS 5.0 software package., Results: 10-20 μg/mL rhAm significantly promoted the adhesion, proliferation and migration of HPDLF and HFF (P<0.05), but no significant difference between two proteins was found (P>0.05)., Conclusions: 25 kDa rhAm and EMPs shows similar biological effects on fibroblast, which indicates that rhAm may play an important role in the periodontal regeneration through the activation of fibroblasts.
- Published
- 2013
26. [Genetic diversity of germplasm resources of Lonicera japonica by AFLP analysis].
- Author
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Guo QM, Wang T, Zhou FQ, Li J, and Zhang YQ
- Subjects
- Amplified Fragment Length Polymorphism Analysis, China, Lonicera classification, Phylogeny, Polymorphism, Restriction Fragment Length, Genetic Variation, Lonicera genetics
- Abstract
Objective: This study aimed to analyze the genetic diversity and genetic relationship of germplasm resources of Lonicera japonica in main producing areas of China and provide reference for developing new varieties of L. japonica., Method: Using 6 primer combinations, 13 germplasm of L. japonica were analyzed by AFLP marker. The genetic distance was worked out by using DPS V3.01 software, and the cluster was conducted based on UPGMA., Result: A total of 435 bands were obtained including 191 polymorphic ones. The average polymorphic frequency was 43.9%. Cluster analysis showed that the relationship of cultivated variety from the same genuine area was near, and the classification result based on AFLP marker of germplasm of L. japonica from Shandong province was basically consistent with those on their morphological character., Conclusion: AFLP marker can indicate the abundant genetic diversity of L. japonica and provide theoretical evidence for reasonable utilization and breeding new cultivar of L. japonica in molecular level.
- Published
- 2012
27. Piperine suppresses tumor growth and metastasis in vitro and in vivo in a 4T1 murine breast cancer model.
- Author
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Lai LH, Fu QH, Liu Y, Jiang K, Guo QM, Chen QY, Yan B, Wang QQ, and Shen JG
- Subjects
- Animals, Breast drug effects, Breast metabolism, Breast pathology, Breast Neoplasms genetics, Cell Line, Tumor, Female, Gene Expression Regulation, Neoplastic drug effects, Mammary Neoplasms, Experimental drug therapy, Mammary Neoplasms, Experimental pathology, Matrix Metalloproteinase 13 genetics, Matrix Metalloproteinase 9 genetics, Mice, Mice, Inbred BALB C, Neoplasm Metastasis prevention & control, Piper chemistry, Alkaloids therapeutic use, Antineoplastic Agents, Phytogenic therapeutic use, Benzodioxoles therapeutic use, Breast Neoplasms drug therapy, Breast Neoplasms pathology, Piperidines therapeutic use, Polyunsaturated Alkamides therapeutic use
- Abstract
Aim: To investigate the effects of piperine, a major pungent alkaloid present in Piper nigrum and Piper longum, on the tumor growth and metastasis of mouse 4T1 mammary carcinoma in vitro and in vivo, and elucidate the underlying mechanisms., Methods: Growth of 4T1 cells was assessed using MTT assay. Apoptosis and cell cycle of 4T1 cells were evaluated with flow cytometry, and the related proteins were examined using Western blotting. Real-time quantitative PCR was applied to detect the expression of matrix metalloproteinases (MMPs). A highly malignant, spontaneously metastasizing 4T1 mouse mammary carcinoma model was used to evaluate the in vivo antitumor activity. Piperine was injected into tumors every 3 d for 3 times., Results: Piperine (35-280 μmol/L) inhibited the growth of 4T1 cells in time- and dose-dependent manners (the IC(50) values were 105 ± 1.08 and 78.52 ± 1.06 μmol/L, respectively, at 48 and 72 h). Treatment of 4T1 cells with piperine (70-280 μmol/L) dose-dependently induced apoptosis of 4T1 cells, accompanying activation of caspase 3. The cells treated with piperine (140 and 280 μmol/L) significantly increased the percentage of cells in G(2)/M phase with a reduction in the expression of cyclin B1. Piperine (140 and 280 μmol/L) significantly decreased the expression of MMP-9 and MMP-13, and inhibited 4T1 cell migration in vitro. Injection of piperine (2.5 and 5 mg/kg) dose-dependently suppressed the primary 4T1 tumor growth and injection of piperine (5 mg/kg) significantly inhibited the lung metastasis., Conclusion: These results demonstrated that piperine is an effective antitumor compound in vitro and in vivo, and has the potential to be developed as a new anticancer drug.
- Published
- 2012
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28. Salvianic acid A inhibits lipopolysaccharide-induced apoptosis through regulating glutathione peroxidase activity and malondialdehyde level in vascular endothelial cells.
- Author
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Zhao QT, Guo QM, Wang P, and Wang Q
- Subjects
- Cell Cycle drug effects, Cell Line, Cell Survival drug effects, Dose-Response Relationship, Drug, Drugs, Chinese Herbal pharmacology, Endothelium, Vascular cytology, Endothelium, Vascular metabolism, Human Umbilical Vein Endothelial Cells, Humans, Lipopolysaccharides, Antioxidants pharmacology, Apoptosis drug effects, Endothelium, Vascular drug effects, Glutathione Peroxidase metabolism, Lactates pharmacology, Malondialdehyde metabolism, Salvia miltiorrhiza chemistry
- Abstract
Aim: To find out the role of salvianic acid A (SAA) in the protection of vascular endothelial cells (VEC) and its possible mechanism in vitro., Methods: The ingredient at various concentrations was added to human umbilical vein endothelial cells (HUVEC) treated with 0.5 μmol·L(-1) lipopolysaccharide (LPS) for 24 h. Apoptotic morphological changes of cells were observed under inverted phase contrast microscope; the cell viability was quantified using MTT assay. Nuclear fragmentation of cells was observed under laser scanning confocal microcope after being stained with acridinorange. Cell cycle distribution was detected by flow-cytometry after being stained with propidium iodide (PI). The activities of glutathione peroxidase (GPH-PX) as well as maleic dialdehyde (MDA) level in cells were measured by spectrophotometric methods as described in the assay kits., Results: Apoptotic morphological changes and the decrease of cell viability of these cells were obviously inhibited by SAA in a dose-dependent manner. Furthermore, the abnormal cell cycle distribution, the decrease of GSH-Px activity and the increase of MDA level induced by LPS were markedly reversed., Conclusion: SAA exerts protective effect on VEC induced by LPS via an antioxidative mechanism., (Copyright © 2012 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.)
- Published
- 2012
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29. In vivo expression of Toll-like receptor 2, Toll-like receptor 4, CSF2 and LY64 in Chinese chronic periodontitis patients.
- Author
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Sun Y, Guo QM, Liu DL, Zhang MZ, and Shu R
- Subjects
- Adult, Aged, Antigens, CD genetics, Case-Control Studies, China, Chronic Periodontitis ethnology, Female, Gingiva metabolism, Gingival Crevicular Fluid metabolism, Granulocyte-Macrophage Colony-Stimulating Factor genetics, Humans, Immunohistochemistry, Male, Middle Aged, Periodontal Index, RNA, Messenger analysis, Reference Values, Toll-Like Receptor 2 genetics, Toll-Like Receptor 4 genetics, Antigens, CD metabolism, Chronic Periodontitis metabolism, Granulocyte-Macrophage Colony-Stimulating Factor metabolism, Toll-Like Receptor 2 metabolism, Toll-Like Receptor 4 metabolism
- Abstract
Objective: Toll-like receptors (TLRs) are the essential components in the innate and adaptive immune systems. Colony stimulating factor 2 (CSF2) is a cytokine that may prevent endotoxin tolerance, and LY64 has the ability to interfere with the recognition of bacteria via TLR4. The aim of this study was to explore the in vivo expressions of TLR2, TLR4, CSF2 and LY64 in Chinese chronic periodontitis patients., Methods: Gingival biopsies were collected from 24 chronic periodontitis patients and 19 healthy controls. The gene expression profiles of TLR2, TLR4, CSF2 and LY64 were investigated by real-time polymerase chain reaction, and the protein expressions of TLR2 and TLR4 were detected by immunohistochemistry. In addition, the levels of CSF2 in gingival crevicular fluid (GCF) were determined by ELISA., Results: The higher mRNA expressions of TLR2, TLR4 and CSF2, and the lower mRNA expression of LY64 were detected in chronic periodontitis patients. And the increased protein expressions of TLR2 and TLR4 were confirmed by immunohistochemistry. In addition, the increase of total amount of CSF2 in GCF was observed in chronic periodontitis patients., Conclusions: Our results suggest that TLR2 and TLR4 may play a role in periodontal pathogenesis. In addition, CSF2 and LY64 may contribute to the regulation of inflammatory response and maintaining periodontal homeostasis.
- Published
- 2010
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30. [Treatment of fracture of multiple ribs with absorbable rib fixed nail and dacron flap in 12 patients].
- Author
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Zhu HH, Xu TZ, Zhou M, and Guo QM
- Subjects
- Adult, Bone Nails, Female, Fracture Fixation, Intramedullary, Humans, Male, Middle Aged, Surgical Flaps, Treatment Outcome, Young Adult, Rib Fractures surgery
- Abstract
Objective: To introduce the methods of dacron flap application and its indications in treating fracture of multiple ribs, in order to reduce the incidence of the complications of fracture displacement., Methods: From September 2006 to March 2008, 12 patients with fracture of multiple ribs were treated with absorbable rib fixed nail and dacron flap. Included 8 males and 4 females,the age was from 22 to 51 years with an average of 38.2 years,the operative time was 2 hours to 3 days after injured. All the patients were closed injury and simultaneously accompanied with significant chest pain and chest tightness. 4 cases with dyspnea, blood in sputum and blood oxygen saturation decreased. The X-ray showed 3 cases of unilateral fracture and 9 cases of bilateral rib fractures and all cases accompanied with hemopneumothorax., Results: All patients were followed up from 2 to 26 months with an average of 8 months. All the fractures healed. According to clinical criteria, pain, breathing, ribs alignment etc. to observe the effect, 10 cases got excellent result, 1 case good, 1 case poor., Conclusion: It is safe and effective to use absorbable rib fixed nails and dacron flap for treating fracture of multiple ribs and especially for the patients of osteoporosis, comminuted fracture or oblique fracture.
- Published
- 2009
31. [Absorption and transport of 6 coumarins isolated from the roots of Angelica pubescens f. biserrata in human Caco-2 cell monolayer model].
- Author
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Yang XW, Guo QM, and Wang Y
- Subjects
- Absorption, Caco-2 Cells, Furocoumarins isolation & purification, Furocoumarins pharmacokinetics, Humans, Umbelliferones isolation & purification, Umbelliferones pharmacokinetics, Angelica chemistry, Coumarins isolation & purification, Coumarins pharmacokinetics, Plant Roots chemistry
- Abstract
Objective: To study the absorption and transepithelial transport of six coumarins (umbelliferone, osthole, columbianadin, columbianetin acetate, angelol-A and angelol-B, isolated from the roots of Angelica pubescens f. biserrata) in the human Caco-2 cell monolayer model., Methods: The in vitro cultured human colon carcinoma cell line, Caco-2 cell monolayer model, was applied to study the absorption and transport of the six coumarins from apical (AP) to basolateral (BL) side and from BL to AP side. The six coumarins were measured by reversed-phase high-performance liquid chromatography (HPLC) coupled with ultraviolet absorption detector. Transport parameters and apparent permeability coefficients (P(app)) were calculated and compared with those of propranolol as a control substance of high permeability and atenolol as a control substance of poor permeability. The transport mechanism of angelol-B was assayed by using iodoacetamide as a reference standard to inhibit ATP-dependent transport and MK571 as a well-known inhibitor of MRP2., Results: The absorption and transport of six coumarins were passive diffusion as the dominating process. The P(app) values of umbelliferone, osthole, columbianadin, columbianetin acetate, angelol-A and angelol-B from AP to BL side were (2.679+/-0.263) x 10(-5), (1.306+/-0.324) x 10(-5), (0.595+/-0.086) x 10(-6), (2.930+/-0.410) x 10(-6), (1.532+/-0.444) x 10(-5) and (1.413+/-0.243) x 10(-5) cm/s, and from BL to AP side were (3.381+/-0.410) x 10(-5), (0.898+/-0.134) x 10(-5), (0.510+/-0.183) x 10(-6), (0.222+/-0.025) x 10(-6), (1.203+/-0.280) x 10(-5) and (0.754+/-0.092) x 10(-5) cm/s, respectively. In this assay, the P(app) value of propranolol was 2.18 x 10(-5) cm/s and the P(app) value of atenolol was 2.77 x 10(-7) cm/s. Among the 6 coumarins, the P(app) values of umbelliferone, osthole, angelol-A and angelol-B from AP to BL side were identical with that of propranolol, and columbianadin and columbianetin acetate lied between propranolol and atenolol. When replaced the HBSS with EBSS, and iodoacetamide or MK-591 were used in the experiment, the P(app) of angelol-B had no statistical difference as compared with the control group. In the mean total recoveries, umbelliferone was (83.31+/-3.52)%, angelol-A was (77.39+/-7.38)%, osthole, columbianadin and angelol-B were between 50% to 65%, and columbianetin acetate was lower than 10%. The accumulation rates of osthole and columbianadin in the Caco-2 cells were (36.15+/-5.87)% and (53.90+/-4.39)%, respectively., Conclusion: The absorption and transport of umbelliferone, osthole, columbianadin, columbianetin acetate, angelol-A and angelol-B are passive diffusion as the dominating process in Caco-2 cell monolayer model. Umbelliferone, osthole, angelol-A and angelol-B are estimated to be highly absorbed compounds, and columbianadin and columbianetin acetate are estimated to be moderately absorbed compounds. In the Caco-2 cells, osthol and columbianadin appear to accumulate, and columbianetin acetate may be metabolized. The absorption and transport of angelol-B are not influenced by the change of pH and the presence of iodoacetamide or MK571.
- Published
- 2008
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32. [Studies on chemical constituents in fruits of Eucalyptus globulus].
- Author
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Yang XW and Guo QM
- Subjects
- Cyclohexanecarboxylic Acids chemistry, Fruit chemistry, Glucosides chemistry, Molecular Structure, Phloroglucinol chemistry, Phloroglucinol isolation & purification, Sesquiterpenes chemistry, Sitosterols chemistry, Sitosterols isolation & purification, Stigmasterol chemistry, Stigmasterol isolation & purification, Cyclohexanecarboxylic Acids isolation & purification, Eucalyptus chemistry, Glucosides isolation & purification, Phloroglucinol analogs & derivatives, Plants, Medicinal chemistry, Sesquiterpenes isolation & purification
- Abstract
Objective: To study the chemical constituents in the fruits of Eucalyptus globulus Labill., Method: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, NMR and MS evidences., Result: Fifteen compounds were obtained and identified as beta-sitosterol (1), betulinic acid (2), stigmasterol (3), euscaphic acid (4), 2a-Hydroxybetulinic acid (5), macrocarpal B (6), macrocarpal A (7), oleanolic acid (8), 3,4,3'-O-trimethylellagic acid (9), 3-O-methylellagic acid 4'-O-(2"-O-acetyl )-alpha-L-rhamnopyranoside (10), camaldulenside (cypellocarpin C, 11), 3-O-methylellagic acid 4'-O-alpha-L-rhamnopyranoside (12), 3-O-methylellagic acid (13), ellagic acid (14), and gallic acid (15)., Conclusion: Compounds 4 and 5 from genera Eucalyptus, 1, 3 and 11 from plant E. globulus, and 6, 7, 9 and 15 from the fruits of E. globulus were isolated for the first time.
- Published
- 2007
33. Intestinal permeability of antivirus constituents from the fruits of Eucalyptus globulus Labill. in Caco-2 Cell Model.
- Author
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Yang XW, Guo QM, Wang Y, Xu W, Tian L, and Tian XJ
- Subjects
- Alkylating Agents chemistry, Antiviral Agents isolation & purification, Biological Availability, Caco-2 Cells, Calcium Channel Blockers pharmacology, Cell Membrane metabolism, Chemical Phenomena, Chemistry, Physical, Chromatography, High Pressure Liquid, Humans, Intestinal Absorption, Iodoacetamide pharmacology, Phloroglucinol analogs & derivatives, Phloroglucinol chemistry, Phloroglucinol pharmacokinetics, Propionates pharmacology, Quinolines pharmacology, Sesquiterpenes chemistry, Sesquiterpenes pharmacokinetics, Verapamil pharmacology, Antiviral Agents pharmacokinetics, Eucalyptus chemistry, Fruit chemistry
- Abstract
The uptake and transepithelial transport of the three main constituents macrocarpal A (M-A), macrocarpal B (M-B), and cypellocarpa C (Cy-C) from the fruits of Eucalyptus globulus Labill. were investigated. Monolayers of the human intestinal epithelial cancer cell line Caco-2 were incubated with M-A, M-B, and Cy-C to model its intestinal absorption and transport, respectively. The determination of compounds was performed by HPLC. The apparent permeability coefficients (P(app)) for M-A, M-B, and Cy-C in the apical-to-basolateral direction of a Caco-2 monolayer were (1.70+/-0.06)x10(-6), (1.99+/-0.10)x10(-6), and (6.08+/-0.41)x10(-6)cm/s, respectively. In the presence of iodoacetamide, the P(app) of Cy-C were both reducted in apical-to-basolateral and basolateral-to-apical directions. M-A and M-B appear to accumulate in the epithelial cells. The intestinal absorption of M-A, M-B, and Cy-C was passive diffusion as the dominating process and Cy-C was partly ATP-dependent.
- Published
- 2007
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34. Jianqu, a traditional Chinese medicine, alleviates functional dyspepsia in high-calorie and high-protein diet mice.
- Author
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Jing-Yan Yang, Xiao-Xing Li, Juan Chen, Yan-Jun Liu, Yue-Hong Wu, Ning-Yu Luo, Cai-Xia Yang, Yang Li, Si-Jing Liu, and Jin-Lin Guo
- Subjects
HIGH-protein diet ,SHORT-chain fatty acids ,HIGH-calorie diet ,CHINESE medicine ,GASTROPARESIS ,INDIGESTION ,APPETITE stimulants ,BUTYRATES - Abstract
Background: Jianqu has been used to alleviate symptoms in patients with functional dyspepsia, but its specific anti-functional dyspepsia effect is still unclear. Therefore, our study aimed to investigate the impact of Jianqu on functional dyspepsia in mice. Methods: The phytochemical profile of Jianqu was analyzed by UPLC-Q-TOF-MS. Subsequently, Kunming mice were fed a high-calorie or high-protein diet (HCHP) for 7 days, and then orally treated with vehicle or Jianqu (1.62 g/kg body weight (b. w.) and 3.25 g/kg b. w.) for 10 days. A carbon powder solution was used to detect the gastric emptying and intestinal transit rate. The pathological changes in stomach and duodenum were evaluated by hematoxylin-eosin staining. The occludin, claudin-1, ZO-1 and CD45 expression was measured by immunocytochemical staining. Importantly, the serum gastrointestinal hormones were detected by ELISA. In addition, the gut microbiota composition was determined using 16S rRNA gene sequencing. The cecal short chain fatty acids were assessed by gas chromatography. Results: In general, 17 phytochemical compounds were identified from Jianqu, which significantly improved the gastric emptying rate and intestinal transit rate (p < 0.01), increased the body weight and food intake (p < 0.0001) in HCHP mice as well. Though HCHP did not cause significant pathological lesions in the gastrointestinal tract, increased the expression of CD45 in the duodenum (p < 0.05) was observed. Notably, Jianqu attenuated this abnormal expression of CD45 (p < 0.05). The levels of serum gastrointestinal hormones were significantly normalized by Jianqu intervention (p < 0.05). Moreover, Jianqu increased the relative abundance of Roseburia as well as short chain fatty acids levels in cecum (p < 0.05). Conclusion: The present results showed that Jianqu alleviated dyspeptic symptoms in HCHP mice possibly through reducing the duodenal leukocyte infiltration, and regulating the expression of gastrointestinal hormones. These effects may be partly related to the increasing cecal short chain fatty acids levels probably via gut microbial modulation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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35. Characterization and in vitro cytotoxicity of piperine-loaded nanoemulsion in breast cancer cells.
- Author
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Vitor, Leticia C., Di Filippo, Leonardo D., Duarte, Jonatas L., Brito, Lorrane D., Luiz, Marcela T., Dutra, Jessyca A. P., Sábio, Rafael M., Haddad, Felipe F., Scarim, Cauê B., da Costa, Paulo Inacio, and Chorilli, Marlus
- Abstract
Piperine is an alkaloid of natural origin with potent anti-cancer activity. However, due to its physicochemical characteristics, piperine's biological performance is limited, with low bioavailability resulting from high lipophilicity and low water solubility. To improve its cytotoxic activity in 4T1 and MCF7 breast cancer cell lines, we developed and characterized piperine-loaded nanoemulsions (Pip-NE). The nanoemulsions were obtained by spontaneous emulsification followed by sonication and were characterized regarding their size by dynamic light scattering techniques and their zeta potential by electrophoretic mobility. Pip-NE showed an average hydrodynamic diameter of 102 nm, polydispersity index of 0.2, and zeta potential of − 31.8 mV. Compared to piperine in solution, Pip-NE showed sustained in-vitro release, preserving the cytotoxic activity of piperine. The formulations presented no irritancy potential in the Hen's Egg Test on Chorioallantoic Membrane assay. Additionally, the nanoemulsification increased the piperine cytotoxicity against both breast cancer cell lines, as evidenced by the IC
50 values. Overall, this study contributes to understanding the potential of drug delivery nanosystems as a novel strategy to optimize the delivery and enhance the cytotoxic properties of lipophilic drugs such as piperine in breast cancer cell lines. [ABSTRACT FROM AUTHOR]- Published
- 2024
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36. [Studies on micromorphological characters of fruit coats in cultivated Fructus Trichosanthis in Shandong Province].
- Author
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Guo QM, Zhou FQ, Yang JL, and Gao H
- Subjects
- China, Microscopy, Electron, Scanning, Plants, Medicinal classification, Plants, Medicinal growth & development, Quality Control, Trichosanthes classification, Trichosanthes growth & development, Fruit ultrastructure, Plants, Medicinal ultrastructure, Trichosanthes ultrastructure
- Abstract
Objective: To explore the affinity relation among land race of Trichosanthes kirilowii and to provide evidence tor the classification and authentication., Method: Using scanning electron microscope, characteristics of the pericarp surface were studied comparatively., Result: The pericarp surface of the cultivated Fructus Trichosanthis in Shandong showed the characteristics of generality and diversity., Conclusion: The results of the study could be used for the identification of the cultivated Fructus Trichosanthis in Shandong.
- Published
- 2005
37. A new ellagic acid derivative from the fruits of Eucalyptus globulus Labill.
- Author
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Guo QM and Yang XW
- Subjects
- Ellagic Acid isolation & purification, Fruit chemistry, Magnetic Resonance Spectroscopy, Mass Spectrometry, Monosaccharides isolation & purification, Plant Extracts analysis, Spectrophotometry, Infrared, Spectroscopy, Fourier Transform Infrared, Ellagic Acid analogs & derivatives, Ellagic Acid chemistry, Eucalyptus chemistry, Monosaccharides chemistry
- Abstract
Four ellagic acid derivatives have been isolated from the fruits of Eucalyptus globulus Labill., one of which is new compound, identified as 3-O-methylellagic acid 4'-O-alpha-L-2"-O-acetyl-rhamnopyranoside (1), the known compounds were identified as 3-O-methylellagic acid 4'-O-alpha-L-rhamnopyranoside (2), ellagic acid (3) and 3-O-methylellagic acid (4), on the basis of the analysis of 1H NMR, 13C NMR, HSQC, HMBC, IR and MS spectral data. It is alsoassignment the 13C NMR signals of 3-O-methylellagic acid 4'-O-alpha-L-rhamnopyranoside for the first.
- Published
- 2005
38. [The relationship of angiotensin I-converting enzyme gene polymorphism with diabetic retinopathy and diabetes myocardial infarction].
- Author
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Wu SS, Guo QM, Liu GL, Zhang J, Zhao CF, Ning SC, Zhao LN, Yu F, and Yi HL
- Subjects
- Adult, Aged, Diabetic Retinopathy etiology, Female, Humans, Male, Middle Aged, Myocardial Infarction etiology, Risk Factors, Diabetes Mellitus, Type 2 complications, Diabetic Retinopathy genetics, Myocardial Infarction genetics, Peptidyl-Dipeptidase A genetics, Polymorphism, Genetic
- Abstract
Objective: To investigate the relationship of angiotensin I-converting enzyme (ACE) gene polymorphism to diabetic retinopathy and diabetes myocardial infarction., Methods: ACE insertion/deletion(I/D) polymorphism was determined by PCR., Results: No evidence showed that ACE gene was associated with diabetic retinopathy. By comparison of the type 2 diabetes patients with myocardial infarction versus those without-myocardial infarction, it was found that the frequencies of homozygote DD (41.2% versus 33.2%) and of allele D (64.7% versus 55.0%) increased remarkably; the difference was statistically significant (P<0.05)., Conclusion: Allele D(RR=1.50) and genotype DD(RR=1.33) seemed to be a genetic risk factor for type 2 diabetes myocardial infarction.
- Published
- 2004
39. High-throughput proteomics for alcohol research.
- Author
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Neuhold LA, Guo QM, Alper J, and Velazquez JM
- Subjects
- Alcohol Drinking metabolism, Alcoholism metabolism, Animals, Computational Biology methods, Humans, Research Design, Societies, Medical, United States, Alcohol Drinking genetics, Alcoholism genetics, Proteomics methods
- Abstract
This report summarizes the proceedings of a satellite symposium of the 2003 Research Society on Alcoholism meeting held on June 21, 2003, in Fort Lauderdale, FL. The goal of this symposium, sponsored by the NIAAA, was to identify new proteomic directions in alcohol research that will (1) enable studies that focus on characterizing protein function, biochemical pathways, and networks to understand alcohol-related illnesses; (2) identify protein-protein interactions, posttranslational modifications, and subcellular localizations; (3) identify molecular targets for medication development; (4) develop biomarkers for susceptibility, dependence, consumption, and relapse, as well as alcohol-induced pathologies; and (5) develop high-throughput drug screens to test the efficacy of therapeutics that control alcohol-induced diseases. The purpose of the symposium was also to promote the application of high-throughput proteomic approaches, including isolation of membrane-bound proteins, in situ proteomics, large-scale two-dimensional separations, protein microarray platforms, mass spectrometry, matrix-assisted laser desorption/ionization, matrix-assisted laser desorption/ionization time-of-flight, liquid chromatography-tandem mass spectrometry, and isotope-coded affinity tags. In addition, the development of protein network maps by using new bioinformatics approaches for database mining was also discussed.
- Published
- 2004
- Full Text
- View/download PDF
40. Relationship between overexpression of NK-1R, NK-2R and intestinal mucosal damage in acute necrotizing pancreatitis.
- Author
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Shi X, Gao NR, Guo QM, Yang YJ, Huo MD, Hu HL, and Friess H
- Subjects
- Amylases blood, Animals, RNA, Messenger metabolism, Random Allocation, Rats, Rats, Sprague-Dawley, Receptors, Neurokinin-1 genetics, Receptors, Neurokinin-2 genetics, Statistics as Topic, Intestinal Mucosa metabolism, Intestinal Mucosa pathology, Pancreatitis, Acute Necrotizing metabolism, Pancreatitis, Acute Necrotizing pathology, Receptors, Neurokinin-1 metabolism, Receptors, Neurokinin-2 metabolism
- Abstract
Aim: To study the expression of neurokinin-1 receptor (NK-1R) and neurokinin-2 receptor (NK-2R) in distal ileum of acute necrotizing pancreatitis (ANP) and to evaluate the relationship between expression of these two receptors and intestinal mucosal damage., Methods: A total of 130 adult Sprague-Dawley rats were randomly divided into two groups: the rats in ANP group (n=80) were induced by the retrograde intraductal infusion of 30 g.L(-1) sodium taurocholate. And the rats in normal control group (n=50) received laparotomy only. Sacrifices were made 6 h, 12 h, 24 h and 48 h later in ANP and normal control group after induction respectively. Intestinal mucosal permeability was studied by intrajejunal injection of 1.5 mCi radioactive isotope (99m)Tc-diethlene triamine pentacetic acid (DTPA) and the radioactivity of (99m)Tc-DTPA content in urine was measured 6 h, 12 h, 24 h and 48 h after induction. Then the pancreas and intestine were prepared for pathology. Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the mRNA expression of NK-1R and NK-2R, and Western blot was used to investigate the protein level of NK-1R and NK-2R., Results: In ANP rats, serious histologic damages in intestinal mucosa were observed, and the radioactivity of (99m)Tc-DTPA in urine increased significantly in the ANP group. RT-PCR revealed that NK-1R and NK-2R mRNA level was overexpressed in the distal ileum of ANP as compared with the normal control group. Western blot discovered stronger NK-1R (14-fold increase) and NK-2R (9-fold increase) immunoreactivity in the intestinal mucosa of ANP rats. Moreover, the overexpression of NK-1R was associated with mucosal pathological score (r=0.77, P<0.01) and intestinal permeability (r=0.68, P<0.01) in ANP rats., Conclusion: NK-1R and NK-2R contribute to disrupted neuropeptides loop balance, deteriorate intestinal damage, and are involved in pathophysiological changes in ANP.
- Published
- 2003
- Full Text
- View/download PDF
41. Identification of Src transformation fingerprint in human colon cancer.
- Author
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Malek RL, Irby RB, Guo QM, Lee K, Wong S, He M, Tsai J, Frank B, Liu ET, Quackenbush J, Jove R, Yeatman TJ, and Lee NH
- Subjects
- Animals, Cell Line, Transformed, Cluster Analysis, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Pyridones pharmacology, Pyrimidines pharmacology, Rats, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation, Cell Transformation, Neoplastic, Colonic Neoplasms genetics, Genes, src
- Abstract
We used a classical rodent model of transformation to understand the transcriptional processes, and hence the molecular and cellular events a given cell undergoes when progressing from a normal to a transformed phenotype. Src activation is evident in 80% of human colon cancer, yet the myriad of cellular processes effected at the level of gene expression has yet to be fully documented. We identified a Src 'transformation fingerprint' within the gene expression profiles of Src-transformed rat 3Y1 fibroblasts demonstrating a progression in transformation characteristics. To evaluate the role of this gene set in human cancer development and progression, we extracted the orthologous genes present on the Affymetrix Hu95A GeneChip (12k named genes) and compared expression profiles between the Src-induced rodent cell line model of transformation and staged colon tumors where Src is known to be activated. A similar gene expression pattern between the cell line model and staged colon tumors for components of the cell cycle, cytoskeletal associated proteins, transcription factors and lysosomal proteins suggests the need for co-regulation of several cellular processes in the progression of cancer. Genes not previously implicated in tumorigenesis were detected, as well as a set of 14 novel, highly conserved genes with here-to-fore unknown function. These studies define a set of transformation associated genes whose up-regulation has implications for understanding Src mediated transformation and strengthens the role of Src in the development and progression of human colon cancer. Supportive Supplemental Data can be viewed at http://pga.tigr.org/PGApubs.shtml.
- Published
- 2002
- Full Text
- View/download PDF
42. Silencing of Wnt signaling and activation of multiple metabolic pathways in response to thyroid hormone-stimulated cell proliferation.
- Author
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Miller LD, Park KS, Guo QM, Alkharouf NW, Malek RL, Lee NH, Liu ET, and Cheng SY
- Subjects
- Animals, Cell Division, Cell Line, Cytoskeletal Proteins physiology, Gene Expression Profiling, Kinetics, Oligonucleotide Array Sequence Analysis, RNA, Messenger biosynthesis, Rats, Transcriptional Activation, Wnt Proteins, beta Catenin, Gene Silencing, Proto-Oncogene Proteins antagonists & inhibitors, Signal Transduction, Trans-Activators, Triiodothyronine pharmacology, Zebrafish Proteins
- Abstract
To investigate the transcriptional program underlying thyroid hormone (T3)-induced cell proliferation, cDNA microarrays were used to survey the temporal expression profiles of 4,400 genes. Of 358 responsive genes identified, 88% had not previously been reported to be transcriptionally or functionally modulated by T3. Partitioning the genes into functional classes revealed the activation of multiple pathways, including glucose metabolism, biosynthesis, transcriptional regulation, protein degradation, and detoxification in T3-induced cell proliferation. Clustering the genes by temporal expression patterns provided further insight into the dynamics of T3 response pathways. Of particular significance was the finding that T3 rapidly repressed the expression of key regulators of the Wnt signaling pathway and suppressed the transcriptional downstream elements of the beta-catenin-T-cell factor complex. This was confirmed biochemically, as beta-catenin protein levels also decreased, leading to a decrease in the transcriptional activity of a beta-catenin-responsive promoter. These results indicate that T3-induced cell proliferation is accompanied by a complex coordinated transcriptional reprogramming of many genes in different pathways and that early silencing of the Wnt pathway may be critical to this event.
- Published
- 2001
- Full Text
- View/download PDF
43. [Effects of opioids on Ca2+/calmodulin dependent protein kinase signal pathway in NG108-15 cells].
- Author
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Guo QM and Liu JS
- Subjects
- Animals, Calmodulin metabolism, Cell Nucleus drug effects, Cell Nucleus metabolism, Cyclic AMP metabolism, Cytoplasm drug effects, Cytoplasm metabolism, Glioma, Hybrid Cells, Mice, Neuroblastoma, Rats, Analgesics, Opioid pharmacology, Calcium-Calmodulin-Dependent Protein Kinases metabolism, Enkephalin, D-Penicillamine (2,5)- pharmacology, Signal Transduction drug effects
- Abstract
Aim: To observe the change of Ca2+/calmodulin dependent protein kinase II (CaMK II) signal pathway in opioid dependent NG108-15 cells., Methods: NG108-15 cells were used as an in vitro model system. Competitive protein binding assay and radioimmunoassay were used to examine the intracellular cAMP accumulation. Calmodulin activity was assayed by PDE method. CaMK II activity was assayed by gamma-32 P incorporation of syntide-2., Results: DPDPE long-term treatment increased calmodulin activity and CaMK II activity in both cytoplasm and nucleus of NG108-15 cells. Specific calmodulin antagonist W-7 was found to significantly inhibit the elevation of calmodulin and CaMK II activity which resulted from DPDPE long-term treatment, and CaMK II inhibitor KN-62 also inhibited elevation of CaMK II activity by DPDPE long-term treatment. When naloxone was added to NG108-15 cells which were long-term treated by DPDPE, calmodulin and CaMK II activity increased, indicating that naloxone withdrawal can increase Ca2+/CaMK II pathway activity., Conclusion: The results indicate that Ca2+/CaMK II pathway was involved in the mechanisms of opioids dependence when DPDPE was long-term administered to NG108-15 cells.
- Published
- 2001
44. [Studies on the chemical constituents of Eclipta prostrata (L)].
- Author
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Zhang JS and Guo QM
- Subjects
- Coumarins chemistry, Coumarins isolation & purification, Molecular Structure, Eclipta chemistry, Plants, Medicinal chemistry
- Abstract
Aim: To study the chemical constituents of Eclipta prostrata (L)., Methods: The constituents of E. prostrata were systematically separated with the Bohlmann method and percolation and hot extraction methods, and various chromatographies. The structures were elucidated by chemical and spectroscopic means., Results: Ten compounds were isolated from the Eclipta prostrata. Their structures were determined as wedelolactone (1), demethylwedelolactone (2), isodemethylwedelolactone (3), alpha-formylterthienyl (4), strychnolactone (5), beta-sitosterol (6), nonacosanol (7), stearic acid (8), lacceroic acid (9), 3,4-dihydoxy benzoic acid (10). Fourteen ocmpounds, including hydrocarbons and its esters were identified by GC-MS from the least polar fractions., Conclusion: Compound 3 is a new coumestan named isodemethylwedelolactone. Compounds 2-10 and compounds characterized by GC-MS analysis were obtained for the first time from Eclipta prostrata.
- Published
- 2001
45. [Treatment of non-invasive aspergillosis of maxillary sinus by functional endoscopic sinus surgery].
- Author
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Feng LR, Tan CQ, and Guo QM
- Subjects
- Adult, Aged, Aspergillosis drug therapy, Female, Humans, Male, Maxillary Sinusitis drug therapy, Middle Aged, Aspergillosis surgery, Endoscopy methods, Maxillary Sinusitis surgery
- Abstract
Objective: To evaluate the treatment of non-invasive aspergillosis of the maxillary sinus by using functional endoscopic sinus surgery (FESS) and the correlated factors that affect the treatment., Method: 41 cases of local, non-invasive aspergillosis of the maxillary sinus undergone FESS were studied., Result: The period of convalescence of double pathway (via ostium of maxillary sinus and canine fossa) was 4.7 weeks while single pathway (via ostium of maxillary sinus only) was 9.3 weeks. There was significant difference (P < 0.01). The period of convalescence of group using anti-fungal drugs to wash the operative cavity was 6.4 weeks while the group without using drugs was 6.7 weeks. There was no statistic significance (P > 0.05)., Conclusion: FESS is effective in treating of non-invasive aspergillosis of the maxillary sinus. The operation of double pathway is superior to that of single pathway. Whether to use antifungal drugs to wash the operative cavity has no obvious effect on the efficacy.
- Published
- 2000
46. Identification of c-myc responsive genes using rat cDNA microarray.
- Author
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Guo QM, Malek RL, Kim S, Chiao C, He M, Ruffy M, Sanka K, Lee NH, Dang CV, and Liu ET
- Subjects
- Animals, Cell Line, DNA, Complementary genetics, Down-Regulation, Fibroblasts physiology, Gene Expression Regulation physiology, Oligonucleotide Array Sequence Analysis, Protein Biosynthesis, Proteins metabolism, Proto-Oncogene Proteins c-myc biosynthesis, Proto-Oncogene Proteins c-myc genetics, Rats, Up-Regulation, Gene Expression Profiling, Genes, myc physiology, Proto-Oncogene Proteins c-myc physiology
- Abstract
c-Myc functions through direct activation or repression of transcription. Using cDNA microarray analysis, we have identified c-Myc-responsive genes by comparing gene expression profiles between c-myc null and c-myc wild-type rat fibroblast cells and between c-myc null and c-myc null cells reconstituted with c-myc. From a panel of 4400 cDNA elements, we found 198 genes responsive to c-myc when comparing wild-type or reconstituted cells with the null cells. The plurality of the named c-Myc-responsive genes that were up-regulated, including 30 ribosomal protein genes, are involved in macromolecular synthesis and metabolism, suggesting a major role of c-Myc in the regulation of protein synthetic and metabolic pathways. When ectopically overexpressed, c-Myc induced a different and smaller set of c-Myc-responsive genes as compared with the physiologically expressed c-Myc condition. Thus, these results from expression profiling suggest a new primary function for c-Myc and raise the possibility that the physiological and transforming functions of c-myc may be separable.
- Published
- 2000
47. Clinical and experimental study on regional administration of phosphorus 32 glass microspheres in treating hepatic carcinoma.
- Author
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Liu L, Jiang Z, Teng GJ, Song JZ, Zhang DS, Guo QM, Fang W, He SC, and Guo JH
- Abstract
AIM:To study the therapeutical effectiveness, dosage range and toxic adverse effects of domestic phosphorus 32 glass microsphere and evaluate its clinical significance.METHODS:I.Fifty two BALB/c tumor bearing male nude mice were allocated into treatment group(n = 38) and control group(n = 14). In the former group different doses of (32) P-GMS were injected into the tumor mass, while in the latter (31)P-GMS or no treatment was given. The experimental animals were sacrificed in batches, and then the tumors and their nearby tissues were examined by light and electron microscopy.II. Through selective catheterization of hepatic artery, (32)P-GMS was infused to 5 healthy domestic pigs in a dosage equivalent to the therapeutic dose for human being, and (31)P-GMS was infused to another 5 healthy domestic pigs. Two pigs infused with contrast medium served as whole course blank controls. One pig from each group was surrendered to euthanasia at week 1, 4, 8 and 16 respectively. The ultrastructural histopath ological changes in liver tissues taken from different sites were evaluated semiquan titatively. III. One hundred and twenty seven times of (32)P-GMS intrahepatic artery interventional therapies were performed on 93 patients with hepatic carcinoma, including 79 cases of primary hepatic carcinoma and 14 cases of secondary hepatic carcinoma. (32)P-GMS (n = 30), and group B,(32)P-GMS and half dose of trans hepatic artery embolization (TAE)(n = 49), and 18 patients with HCC by TAE only as control group C. Fourteen patients with secondary hepatic carcinoma were treated in the same way as group B or C.RESULTS:I.Comparing with the control group, the treatment group of tumor bearing nude mice attained the tumor inhibition rates of 59.7%-93.7% (F = 579.62 P < 0.01) at 14d. At an absorbed dose of 7320Gy, the tumor cells were completely destroyed. When the absorbed doses ranged from 1830Gy to 3660Gy, most of the tumor cells showed the evidences of injury or necrosis, but there appeared some well differentiated tumor cells and enhanced effect of the autoimmunocytes. At an absorbed dose of 366Gy or less, some tumor cells still remained active prolix-ferative ability. The definite anticancer effect appeared as early as 3d after intratumoral injection of (32)P-GMS.II. The cumulative amount of (32) P-GMS in the target tissue after trans hepatic artery instillation attained more than 90% of the total dose administrated. Semiquantitative analysis of ultrastructral morphology in the experimental group showed no statistical difference between the nuclear abnormality (N(abn)) and mitochondrial variability (M(var)) at week 1 or 2, but revealed prominent difference (X(2) = 6.70-9.68, P < 0.01, X(2) = 65.09-115.09, P <0.001) as compared with those in the other groups. In the experimental group the N abn in tissues showed no significant difference between week 8 and week 16. No apparent changes were found in the stomach, spleen, kidney and lung tissues of the experimental pigs. III. The therapeutical results of HCC patients in group A were closely approximated to those of group C, no hematological toxic side effects were noted, and the systemic reaction was mild. In some patients 2mos-3mos after treatment some secondary foci appeared around the periphery of the primary lesion. In general better effectiveness was obtained in patients with small lesion. After analyzing by RIDIT method, the therapeutic result in group B was significantly better than that in group C, and secondary foci around the original lesion were rarely seen at 3mos after treatment. In group C the collateral circulation was reestablished along the periphery of primary foci and the secondary foci appeared more frequently, and were required to undergo several courses of treatment. In group B, 4 cases of HCC were treated surgically as their mass decreased in size after (32)P-GMS treatment.Resected specimens showed that the tumor was encapsulated by fibrotic tissue and most of the tumor cells necrosed. The 3 year survival rates were 43.3%-51.0% after A and B regimen treatment. In 14 cases of secondary HCC, the foci were well controled within one year after treatment.CONCLUSION:When the experimental model of implanted human liver cancer cells received (32)P-GMS of 1830Gy-3660Gy, it produced excellent anticancer effect without any injury to the normal neighboring tissues and the prominent anticancer effect was shown within 3d after intratumoral injection. Intrahepatic arterial administration of (32)P-GMS at the macro-cosmic absorbed dosage less than 190 Gy/dose exerted reversible sub lethal injury to domestic pig liver tissues. It took more than 8 weeks to repair the injured liver tissue and restore its function.(32)P-GMS trans hepatic artery embolization is an effective and safe regimen in treating hepatic carcinoma.
- Published
- 1999
- Full Text
- View/download PDF
48. [An experimental study of force produced by torquing auxiliary arch in the Begg technique].
- Author
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Guo QM
- Subjects
- Biomechanical Phenomena, Dental Stress Analysis, Humans, Orthodontic Wires, Orthodontics, Corrective methods
- Published
- 1994
49. Successful Management of Malignant Pericarditis Using Nivolumab for Metastatic Esophageal Squamous Cell Carcinoma.
- Author
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Yu Uneno, Motoo Nomura, Taisuke Hosokai, Yoshinosuke Kurakake, Masayuki Fuki, Hiroki Shiomi, Yasuhide Takeuchi, Masashi Tamaoki, Akira Yokoyama, Chikatoshi Katada, and Manabu Muto
- Published
- 2024
- Full Text
- View/download PDF
50. Efficacy of third-generation epidermal growth factor receptor-tyrosine kinase inhibitors in advanced NSCLC with different T790M statuses tested via digital droplet polymerase chain reaction ddPCR and next-generation sequencing.
- Author
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Xu, Ziyi, Li, Yan, Wang, Lin, Hao, Xuezhi, Ying, Jianming, Li, Junling, and Xing, Puyuan
- Subjects
EPIDERMAL growth factor ,POLYMERASE chain reaction ,NUCLEOTIDE sequencing ,EPIDERMAL growth factor receptors ,KINASE inhibitors - Abstract
We hypothesize that digital droplet polymerase chain reaction (ddPCR) would optimize the treatment strategies in epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) relapsed patients. In this study, we compared the efficacy of third-generation TKIs with various T790M statuses via ddPCR and next-generation sequencing (NGS). NGS was performed on blood samples of patients progressed from previous EGFR-TKIs for resistance mechanism. T790M-negative patients received further liquid biopsy using ddPCR for T790M detection. A cohort of 40 patients were enrolled, with 30.0% (12/40) T790M-positive via NGS (Group A). In another 28 T790M-negative patients by NGS, 11 (39.3%) were T790M-positive (Group B) and 17 (60.7%) were T790M-negative (Group C) via ddPCR. A relatively longer progression-free survival (PFS) was observed in group A (NR) and group B (10.0 months, 95% CI 7.040–12.889) than in group C (7.0 months, 95% CI 0.000–15.219), with no significant difference across all three groups (p = 0.196), or between group B and C (p = 0.412). EGFR-sensitive mutation correlated with inferior PFS (p = 0.041) and ORR (p = 0.326), and a significantly lower DCR (p = 0.033) in T790M-negative patients via NGS (n = 28). This study indicates that ddPCR may contribute as a supplement to NGS in liquid biopsies for T790M detection in EGFR-TKIs relapsed patients and help to optimize the treatment strategies, especially for those without coexistence of EGFR-sensitive mutation. identifier is NCT05458726. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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