Back to Search
Start Over
In vivoexpression of Toll-like receptor 2, Toll-like receptor 4, CSF2 and LY64 in Chinese chronic periodontitis patients
- Source :
- Oral Diseases. 16:343-350
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- Oral Diseases (2010) 16, 343–350 Objective: Toll-like receptors (TLRs) are the essential components in the innate and adaptive immune systems. Colony stimulating factor 2 (CSF2) is a cytokine that may prevent endotoxin tolerance, and LY64 has the ability to interfere with the recognition of bacteria via TLR4. The aim of this study was to explore the in vivo expressions of TLR2, TLR4, CSF2 and LY64 in Chinese chronic periodontitis patients. Methods: Gingival biopsies were collected from 24 chronic periodontitis patients and 19 healthy controls. The gene expression profiles of TLR2, TLR4, CSF2 and LY64 were investigated by real-time polymerase chain reaction, and the protein expressions of TLR2 and TLR4 were detected by immunohistochemistry. In addition, the levels of CSF2 in gingival crevicular fluid (GCF) were determined by ELISA. Results: The higher mRNA expressions of TLR2, TLR4 and CSF2, and the lower mRNA expression of LY64 were detected in chronic periodontitis patients. And the increased protein expressions of TLR2 and TLR4 were confirmed by immunohistochemistry. In addition, the increase of total amount of CSF2 in GCF was observed in chronic periodontitis patients. Conclusions: Our results suggest that TLR2 and TLR4 may play a role in periodontal pathogenesis. In addition, CSF2 and LY64 may contribute to the regulation of inflammatory response and maintaining periodontal homeostasis.
- Subjects :
- Adult
Male
China
medicine.medical_treatment
Gingiva
Biology
Pathogenesis
Immune system
Antigens, CD
Reference Values
medicine
Humans
RNA, Messenger
Receptor
General Dentistry
Aged
Toll-like receptor
Granulocyte-Macrophage Colony-Stimulating Factor
Gingival Crevicular Fluid
Middle Aged
medicine.disease
Immunohistochemistry
Chronic periodontitis
Toll-Like Receptor 2
Toll-Like Receptor 4
TLR2
Cytokine
Otorhinolaryngology
Case-Control Studies
Chronic Periodontitis
Immunology
TLR4
Female
Periodontal Index
Subjects
Details
- ISSN :
- 16010825 and 1354523X
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Oral Diseases
- Accession number :
- edsair.doi.dedup.....c25b27f82020ad4f6ad86f399fbeca87