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Introduction: The study aimed to analyze the characteristics of patients admitted to the neurology department of a tertiary hospital who subsequently died, focusing on those with high disease severity. Materials and methods: We conducted a retrospective cohort study of patients who died among those admitted to the neurology department of a regional tertiary hospital from 2013 to 2021. Clinical, radiological, and laboratory results of the included patients were collected, and their primary diagnoses, duration from time of admission to death, and direct causes of death were analyzed. Furthermore, the patients were categorized into subgroups based on sex (male and female), primary diagnosis (ischemic and non-ischemic stroke), and cancer diagnosis for comparative analysis. Results: Of 187 deaths, the primary diagnoses were ischemic stroke (131 cases), seizures (19 cases), encephalitis and encephalopathy (18 cases), and other conditions (19 cases). The direct causes of death included ischemic stroke in 68 patients, sepsis in 33, cerebral hemorrhage in 19, pneumonia in 15, acute kidney injury in nine, status epilepticus in seven, and other causes in 36. Pneumonia, cerebral hemorrhage, acute kidney injury, and status epilepticus were the more prevalent direct causes of death in men, whereas ischemic stroke and sepsis were more prevalent in women. Additionally, sepsis, pneumonia, acute kidney injury, and status epilepticus, as direct causes of death, were significantly higher among patients with a primary diagnosis of non-ischemic stroke than among those with a primary diagnosis of ischemic stroke. Furthermore, there were differences in some pre-existing diseases and laboratory findings when comparing between the cancer group and the non-cancer group. Discussion: Ischemic stroke was the primary diagnosis and direct cause of death in a high proportion of patients. Other noteworthy direct causes of death were cerebral hemorrhage and infections such as sepsis and pneumonia. Based on these findings, the characteristics and prognoses of patients admitted to neurology departments can be predicted and used for management. [ABSTRACT FROM AUTHOR]

Objective: Dengue fever is a rapidly spreading viral disease transmitted by mosquitoes, and it is becoming a global concern. This study aimed to assess the awareness, attitude, and performance of the people in Hormozgan province in carrying out preventive behaviors and dengue fever control methods. Method: This descriptive-analytical study was conducted in 1401 in Bandar Abbas, Iran. A total of 642 men and women participated in the study, and a researcher-made questionnaire was used to collect data on demographic information, awareness, attitude, and performance. The data were analyzed using descriptive statistics. Findings: The study included 642 citizens of Bandar Abbas, with the majority being male. The average scores for awareness, attitude, and performance were relatively low, indicating insufficient knowledge and weak preventive behaviors regarding dengue fever. Conclusion: The findings suggest a need for educational programs to increase awareness and preventive behaviors among the citizens, especially in essential areas such as protected coverage against mosquito bites and inspection of water accumulation sites in homes. It is also important for health centers and mass media to provide more information and education about dengue fever and its prevention. [ABSTRACT FROM AUTHOR]

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Natural history collections are invaluable reference collections. Digitizing these collections is a transformative process that improves the accessibility, preservation, and exploitation of specimens and associated data in the long term. Arthropods make up the majority of zoological collections. However, arthropods are small, have detailed color textures and share small, complex and shiny structures, which poses a challenge to conventional digitization methods. Sphaeroptica is a multi-images viewer that uses a sphere of oriented images. It allows the visualization of insects including their tiniest features, the positioning of landmarks, and the extraction of 3D coordinates for measuring linear distances or for use in geometric morphometrics analysis. The quantitative comparisons show that the measures obtained with Sphaeroptica are similar to the measurements derived from 3D μCT models with an average difference inferior to 1%, while featuring the high resolution of color stacked pictures with all details like setae, chaetae, scales, and other small and/or complex structures. Shaeroptica was developed for the digitization of small arthropods but it can be used with any sphere of aligned images resulting from the digitization of objects or specimens with complex surface and shining, black, or translucent texture which cannot easily be digitized using structured light scanner or Structure-from-Motion (SfM) photogrammetry. [ABSTRACT FROM AUTHOR]

Antidepressant response is a multifactorial process related to biological and environmental factors, where brain-derived neurotrophic factor (BDNF) may play an important role in modulating depressive and anxious symptoms. We aimed to analyze how BDNF impacts antidepressant response, considering the levels of anxiety. Methods: A total of 40 depressed adults were included. We evaluated initial serum BDNF, anxiety through the State–Trait Anxiety Inventory (STAI), and the severity of depressive symptoms by the Hamilton Depression Rating Scale (HDRS). Participants received antidepressant treatment for 8 weeks, and response to treatment was evaluated according to the final HDRS scores. Results: Basal BDNF was higher in responders compared to non-responder depressed patients, in addition to being inversely associated with the severity of anxiety and depression. Conclusions: Baseline BDNF serum is an adequate predictive factor for response to antidepressant treatment with SSRI, with lower pre-treatment levels of BDNF associated with higher anxiety symptoms after treatment. Stress levels could influence the response to treatment, but its association was not conclusive. [ABSTRACT FROM AUTHOR]

Supplementation with antioxidant vitamins is common among amateur and elite athletes. It is thought to cause a reduction in the muscle damage that results from exercised-induced oxidative stress, increasing the ability to improve performance markers. A systematic review and meta-analysis were carried out to evaluate the effects of supplementation with antioxidant vitamins (A, C, and E), alone and/or combination of sports performance (maximum oxygen consumption, maximum strength) in athletic populations. Eligibility criteria included only randomized clinical trial studies written in English, Spanish and Portuguese with no restriction on the year of publication. Case studies, observational studies, quasi-experimental trials, literature reviews, and animal studies were excluded from this systematic review. Eligible studies were searched on PubMed/Medline, Web of Science, SciElo, and Google Scholar. To minimize the risk of publication bias, searches were also conducted in the grey literature. Database searches occurred from June to September of 2022. Thirteen randomized controlled trials with a total of 398 participants. The risk of bias was assessed and presented low overall quality. In general, markers of sports performance and overall performance were not affected by antioxidant vitamin supplementation (SMD = 0.00, 95%CI − 0.43, 0.20, P = 0.98). Similarly, evaluations of aerobic resistance (SMD = − 0.06, 95%CI − 0.31, 0.19, P = 0.66) as well as muscle strength (SMD = 0.04, 95% CI − 0.31, 0.39, P = 0.81) were not influenced by supplementation. Thus, the results of this meta-analysis of clinical trials demonstrate that supplementation with isolated and/or combined antioxidant vitamins is not associated with improved sports performance. [ABSTRACT FROM AUTHOR]

Background and Aims: Dental caries and periodontal disease are the most common chronic diseases. Health care workers specially “Behvarz” due to their wide range of duties, have the potential role in delivering education and prevention of dental caries. The purpose of the present study was to assess the perspective of “Behvarz” working in health care centers of Kordkooy city regarding oral health. Materials and Methods: Focus group discussions was conducted with a convenient sample of “Behvarz” working in health centers of Kordkooy city. Total number of 26 Behvarz with the mean work experience of 13 years took part in the study. After obtaining informed consent, group discussions performed in- person (2 groups of 7 and 2 groups of 6 participants) in health centers considering universal precautions. Two researchers coordinated the discussions. Five questions proposed on dental service utilization and oral health challenges and data collection continued until saturation obtained. The content of the discussion recorded and transcribed verbatim. For content analysis, two raters independently coded the content; sub-themes and themes were then extracted and consensus obtained. Results: More than half of the participants were 34 years of age and older. Regarding the challenges related to dental care in health centers, the main themes were; resource limitation, workload, lack of awareness of “Behvarz” role, limited access, economic barriers to prevention and care. Regarding the challenges to prevention, the main themes were; poor knowledge of patients, limited oral health related knowledge of “Behvarz”, and human resources. The main suggestions were emphasizing on the role of schools, mothers and patient education. Conclusion: Our research showed that despite the important role of “Behvarz” in delivering oral health promotion at the population level, this role is neglected and needs further attention. [ABSTRACT FROM AUTHOR]

Introduction and aim. In this review, we suggest ways to improve diabetes mellitus (DM) care in Nigeria from a Health Promotion and Education (HPE) perspective by addressing the gap in DM care through the adoption of strategies from the Ottawa Charter and National Health Promotion Policy (NHPP) guidelines. Material and methods. This review conducted a comprehensive literature search on Africa Journal Online, PubMed, Google Scholar, and Science Direct, from 1986 to 2023, using relevant keywords. Analysis of the literature. The adoption of the Ottawa charter and NHPP remains a key strategy in addressing the gap in DM care in Nigeria. This could be achieved by the adoption of population-focused multi-sectoral interventions encompassing legislation, regulation, and fiscal measures, creating sustaining and expanding health-promoting environments to reduce modifiable risk factors, and reorienting the primary health care services to aid the diagnosis, treatment and rehabilitation of DM patients. Conclusion. This review concluded that the government and other critical stakeholders should adopt the HPE strategies that covers increased financing, strict legislation on DM modifiable risk factors, reorientation of the primary healthcare system, and capacity building for HPE practitioners into DM care in Nigeria as a strategy to improving DM care and prevention in Nigeria. [ABSTRACT FROM AUTHOR]

Brain-derived neurotrophic factor is a key factor in stress adaptation and avoidance of a social stress behavioral response. Recent studies have shown that brain-derived neurotrophic factor expression in stressed mice is brain region-specific, particularly involving the corticolimbic system, including the ventral tegmental area, nucleus accumbens, prefrontal cortex, amygdala, and hippocampus. Determining how brain-derived neurotrophic factor participates in stress processing in different brain regions will deepen our understanding of social stress psychopathology. In this review, we discuss the expression and regulation of brain-derived neurotrophic factor in stress-sensitive brain regions closely related to the pathophysiology of depression. We focused on associated molecular pathways and neural circuits, with special attention to the brain-derived neurotrophic factor-tropomyosin receptor kinase B signaling pathway and the ventral tegmental area-nucleus accumbens dopamine circuit. We determined that stress-induced alterations in brain-derived neurotrophic factor levels are likely related to the nature, severity, and duration of stress, especially in the above-mentioned brain regions of the corticolimbic system. Therefore, BDNF might be a biological indicator regulating stress-related processes in various brain regions., (Copyright © 2025 Copyright: © 2025 Neural Regeneration Research.)

With their unique colony structure, competition between ants (Hymenoptera: Formicidae) can be particularly intense, with colonies potentially willing to sacrifice large number of individuals to obtain resources or territory under the right circumstances. In this review, we cover circumstances in which ant competition escalates into combat, battle strategies and tactics, and analysis methods for these battles. The trends for when colonies choose to fight can vary greatly dependent on the species and situation, which we review in detail. Because of their large group sizes, ant conflicts can follow different patterns than many other species, with a variety of specialist adaptations and battle strategies, such as specialized worker classes and the need to rapidly recruit large number of compatriots. These same large group sizes also can make ant fighting amenable to mathematical analysis, particularly in the context of Lanchester's laws that consider how total numbers influence the outcome of a confrontation. Yet, dynamic behavior can often disrupt idealized mathematical predictions in real-world scenarios, even though these can still shed light on the explanations for such behavior. We also systematically cover the literature on battles between groups of ants, presenting several other interesting studies on species with unique colony organization, such as army ants and leafcutter ants. [ABSTRACT FROM AUTHOR]

Ants are crucial ecosystem engineers, and their ecological success is facilitated by a division of labour among sterile "workers". In some ant lineages, workers have undergone further morphological differentiation, resulting in differences in body size, shape, or both. Distinguishing between changes in size and shape is not trivial. Traditional approaches based on allometry reduce complex 3D shapes into simple linear, areal, or volume metrics; modern approaches using geometric morphometrics typically rely on landmarks, introducing observer bias and a trade‐off between effort and accuracy. Here, we use a landmark‐free method based on large deformation diffeomorphic metric mapping (LDDMM) to assess the co‐variation of size and 3D shape in the mandibles and head capsules of Atta vollenweideri leaf‐cutter ants, a species exhibiting extreme worker size‐variation. Body mass varied by more than two orders of magnitude, but a shape atlas created via LDDMM on μ‐CT‐derived 3D mesh files revealed only two distinct head capsule and mandibles shapes—one for the minims (body mass < 1 mg) and one for all other workers. We discuss the functional significance of the identified 3D shape variation, and its implications for the evolution of extreme polymorphism in Atta. [ABSTRACT FROM AUTHOR]

Animal performance fundamentally influences behaviour, ecology, and evolution. It typically varies monotonously with size. A notable exception is maximum running speed; the fastest animals are of intermediate size. Here we show that this peculiar allometry results from the competition between two musculoskeletal constraints: the kinetic energy capacity, which dominates in small animals, and the work capacity, which reigns supreme in large animals. The ratio of both capacities defines the physiological similarity index Γ, a dimensionless number akin to the Reynolds number in fluid mechanics. The scaling of Γ indicates a transition from a dominance of muscle forces to a dominance of inertial forces as animals grow in size; its magnitude defines conditions of “dynamic similarity“ that enable comparison and estimates of locomotor performance across extant and extinct animals; and the physical parameters that define it highlight opportunities for adaptations in musculoskeletal “design” that depart from the eternal null hypothesis of geometric similarity. The physiological similarity index challenges the Froude number as prevailing dynamic similarity condition, reveals that the differential growth of muscle and weight forces central to classic scaling theory is of secondary importance for the majority of terrestrial animals, and suggests avenues for comparative analyses of locomotor systems.How fast can animals run? Here, the authors show that maximum running speed is limited by different musculoskeletal constraints across animal size: kinetic energy capacity in small animals, and work capacity in large animals. [ABSTRACT FROM AUTHOR]

Objectives: Community Health Workers (CHWs) are important healthcare professionals and key members of team. The purpose of this research is to identify the roles and responsibilities of CHWs in developed and developing countries who provide healthcare assistance to pregnant and lactating women. Methods: For this particular study, a comparison was conducted between CHWs role in seven developed countries, seven South Asian developing countries, and India, with special emphasis on improving maternal health status. Results: CHW programs are essential in communities, institutional health programs, and outreach delivery systems. Without active community involvement, CHWs cannot reach their full potential. Developed countries have frameworks for CHWs, such as the Swasthya Shebika Program, Village Health Worker Cadret, Lady Health Worker Programme, and Accredited Social Health Activist program. CHWs are well-paid in developed nations and work with marginalized groups to spread health messages. However, up to 60% of community health workers in low- and lower-middle-income countries do not receive remuneration. Conclusion: Health systems must support CHWs in choosing technical interventions and providing necessary training, supervision, and logistical support. [ABSTRACT FROM AUTHOR]

Leaf-cutter ants (Atta and Acromyrmex species) exhibit complex social organizations that have fascinated scientists for decades. The leaf-cutter ants belong to a subgroup of fungus-growing ants, which live with fungus inside their nests. The behaviours exhibited by these ants are closely linked to their social organization, which involves intricate division of labour, caste systems, and cooperative tasks. This review article provides an overview of the behaviors associated with the social organization of leaf-cutter ants. It explores various aspects of their social organization, including foraging behavior, hitchhiking behaviour, hygienic behaviour, social organization and environmental influences. The leaf cutter ants have the instinct to forage, as they walk around the nest to cut leaf fragments from plants, then transport those fragments with their jaws and go back to the nest to cultivate a special fungus garden within the colony. By synthesizing existing knowledge, this review highlights the intricate relationships between behaviors and the social structure of leaf-cutter ants, shedding light on the underlying mechanisms and evolutionary implications. Furthermore, it identifies research gaps and proposes future directions for studying leaf-cutter ant behaviors, including emerging techniques and interdisciplinary approaches. The behaviors correlating with the social organization of leaf-cutter ants showcase the intricate mechanisms underlying their highly organized societies. In conclusion, this review contributes to our understanding of the fascinating behaviors exhibited by leaf-cutter ants and their implications in the broader context of social insect societies. [ABSTRACT FROM AUTHOR]

Background. Antihypertensive therapy is crucial for preventing stroke in hypertensive patients. However, the efficacy of antihypertensive therapy varies across individuals, partially due to therapy-related genetic variations among individuals. We investigated the association of antihypertensive drug-related gene polymorphism with stroke in patients with hypertension. Methods. Demographic information, medication, and outcome data were obtained from a hypertensive patient management system, and a PCR fluorescence probe technique was used to detect 7 gene polymorphic loci (CYP2D6 ∗ 10, ADRB1, CYP2C9 ∗ 3, AGTR1, ACE, CYP3A5 ∗ 3, and NPPA), and these loci were compared between patients with and without stroke. Logistic regression was performed to analyze the association of these genetic variations with stroke risk in hypertensive patients while controlling for potential factors. Results. The prevalence of stroke in the hypertensive population in Changsha County of Hunan Province was 2.75%. The mutation frequencies of ADRB1 (1165G > C), CYP2D6 ∗ 10, CYP2C9 ∗ 3, AGTR1 (1166A > C), ACE (I/D), NPPA (2238T > C), and CYP3A5 ∗ 3 were 74.43%, 57.23%, 4.26%, 5.71%, 31.62%, 1.17%, and 69.58%, respectively. Univariate analysis revealed that ADRB1 polymorphism was associated with stroke (χ2 = 8.659, P < 0.05), with a higher stroke risk in the CC group than in the GC and GG groups (GC + GG). Multivariate unconditional logistic regression analysis showed that the CC genotype in ADRB1 (vs. the GC + GG genotype) was associated with an increased risk of stroke [odds ratio (OR) = 1.184, P<0.05] in hypertensive patients. No association was observed between CYP2D6 ∗ 10, CYP2C9 ∗ 3, AGTR1 (1166A > C), ACE (I/D), CYP3A5 ∗ 3, and NPPA (2238T > C) polymorphisms and stroke. Conclusions. ADRB1 (1165G > C) gene polymorphism is associated with the risk of stroke in Chinese hypertensive patients. The CC genotype is correlated with a higher risk of stroke than the GC + GG genotype. [ABSTRACT FROM AUTHOR]

Bite force is a decisive performance trait in animals because it plays a role for numerous life history components such as food consumption, inter- and intraspecific interactions, and reproductive success. Bite force has been studied across a wide range of vertebrate species, but only for 32 species of insects, the most speciose animal lineage. Here we present the insect bite force database with bite force measurements for 654 insect species covering 476 genera, 111 families, and 13 orders with body lengths ranging from 3.76 to 180.12 mm. In total we recorded 1906 bite force series from 1290 specimens, and, in addition, present basal head, body, and wing metrics. As such, the database will facilitate a wide range of studies on the characteristics, predictors, and macroevolution of bite force in the largest clade of the animal kingdom and may serve as a basis to further our understanding of macroevolutionary processes in relation to bite force across all biting metazoans. [ABSTRACT FROM AUTHOR]

Background: Cognitive functional ability affects the accessibility of IT and is thus something that should be controlled for in user experience (UX) research. However, many cognitive function assessment batteries are long and complex, making them impractical for use in conventional experimental time frames. Therefore, there is a need for a short and reliable cognitive assessment that has discriminant validity for cognitive functions needed for general IT tasks. One potential candidate is the Trail Making Test (TMT). Objective: This study investigated the usefulness of a digital TMT as a cognitive profiling tool in IT-related UX research by assessing its predictive validity on general IT task performance and exploring its discriminant validity according to discrete cognitive functions required to perform the IT task. Methods: A digital TMT (parts A and B) named Axon was administered to 27 healthy participants, followed by administration of 5 IT tasks in the form of CAPTCHAs (Completely Automated Public Turing tests to Tell Computers and Humans Apart). The discrete cognitive functions required to perform each CAPTCHA were rated by trained evaluators. To further explain and cross-validate our results, the original TMT and 2 psychological assessments of visuomotor and short-term memory function were administered. Results: Axon A and B were administrable in less than 5 minutes, and overall performance was significantly predictive of general IT task performance (F 5,19=6.352; P =.001; Λ=0.374). This result was driven by performance on Axon B (F 5,19=3.382; P =.02; Λ=0.529), particularly for IT tasks involving the combination of executive processing with visual object and pattern recognition. Furthermore, Axon was cross-validated with the original TMT (P corr=.001 and P corr=.017 for A and B, respectively) and visuomotor and short-term memory tasks. Conclusions: The results demonstrate that variance in IT task performance among an age-homogenous neurotypical population can be related to intersubject variance in cognitive function as assessed by Axon. Although Axon's predictive validity seemed stronger for tasks involving the combination of executive function with visual object and pattern recognition, these cognitive functions are arguably relevant to the majority of IT interfaces. Considering its short administration time and remote implementability, the Axon digital TMT demonstrates the potential to be a useful cognitive profiling tool for IT-based UX research. [ABSTRACT FROM AUTHOR]

Aim: To assist policy-makers in improving access to eye care in under-served areas by analysing the relationship between motivational factors affecting the uptake of task-shifting in eye care and the recruitment and retention of optometrists in remote and rural areas. Background: The World Health Organization recommends two key strategies in tackling preventable blindness in under-served areas: improving human resources for health and task-shifting. The relationship between task-shifting and recruitment and retention of eye care workers in under-served areas is unknown. Ghana and Scotland are two countries from different levels of economic development that have notably expanded the roles of optometrists and struggle with rural recruitment and retention. Methods: Motivation was explored through semi-structured interviews with 19 optometrists in Ghana and Scotland with experience in remote and rural practice. Framework analysis was used to analyse interviews, explore the relationship between task-shifting and recruitment and retention and create recommendations for policy. Findings: The main motivational considerations included altruism, quality of life, learning and career opportunities, fulfilling potential, remuneration, stress of decision-making and collaboration. Motivational and demotivational factors for task-shifting and recruitment/retention shared many similar aspects. Discussion: Recruitment and retention in remote and rural areas require staff be incentivised to take up those positions, motivated to remain and given the adequate resources for personal and professional fulfilment. Task-shifting also requires incentivisation, motivation to continue and the resources to be productive. Many motivational factors influencing recruitment/retention and task-shifting are similar suggesting these two strategies can be compatible and complementary in improving access to eye care, although some factors are culture and context specific. Understanding optometrists' motivation can help policy-makers improve rural recruitment and retention and plan services. [ABSTRACT FROM AUTHOR]

How animals process and absorb nutrients from their food is a fundamental question in biology. Despite the continuity and interaction between intraoral food processing and post-oesophageal nutritional extraction, these topics have largely been studied separately. At present, we lack a synthesis of how pre- and post-oesophageal mechanisms of food processing shape the ability of various taxa to effectively assimilate nutrients from their diet. The aim of this special issue is to catalyse a unification of these distinct approaches as a functional continuum. We highlight questions that derive from this synthesis, as well as technical advances to address these questions. At present, there is also a skew toward vertebrates in studies of feeding form–function mechanics; by including perspectives from researchers working on both vertebrates and invertebrates, we hope to stimulate integrative and comparative research on food processing and nutritional assimilation. Below, we discuss how the papers in this issue contribute to these goals in three areas: championing a functional-comparative approach, quantifying performance and emphasizing the effects of life history, and food substrate and extrinsic factors in current and future studies of oral food processing and nutritional assimilation. This article is part of the theme issue 'Food processing and nutritional assimilation in animals'. [ABSTRACT FROM AUTHOR]

Ants are a dominant family of eusocial terrestrial insects with a diversity of ecologies, lifestyles and morphologies. Ant diet preferences range from strict carnivory through omnivory to almost complete herbivory in species feeding on seeds or exudates of plant-sucking insects. While several studies have investigated ant feeding performance on different substrates, comparatively little is known about the functional morphology of the structures involved in food uptake or their diversification across the ants. To take stock of our current knowledge, we give an overview of how adult ants ingest food, followed by a morphological description of the mouthparts, preoral space and cephalic sucking pump. The mandibles are the most prominent mouthparts and have received considerable attention in the literature, so we focus on the maxillae and labium here. We present current hypotheses for the movement patterns of these parts and discuss morphological differences among ants that may be related to their ecological diversity. Finally, we give short comparisons of the ant condition with some other insects and vertebrates, as well as an outlook summarizing gaps in our knowledge. This sets the stage for future studies elucidating the connections between ant feeding mechanisms and mouthpart evolution. This article is part of the theme issue 'Food processing and nutritional assimilation in animals'. [ABSTRACT FROM AUTHOR]

Herbivores large and small need to mechanically process plant tissue. Their ability to do so is determined by two forces: the maximum force they can generate, and the minimum force required to fracture the plant tissue. The ratio of these forces determines the relative mechanical effort; how this ratio varies with animal size is challenging to predict. We measured the forces required to cut thin polymer sheets with mandibles from leaf-cutter ant workers which vary by more than one order of magnitude in body mass. Cutting forces were independent of mandible size, but differed by a factor of two between pristine and worn mandibles. Mandibular wear is thus likely a more important determinant of cutting force than mandible size. We rationalize this finding with a biomechanical analysis, which suggests that pristine mandibles are ideally 'sharp'—cutting forces are close to a theoretical minimum, which is independent of tool size and shape, and instead solely depends on the geometric and mechanical properties of the cut tissue. The increase of cutting force due to mandibular wear may be particularly problematic for small ants, which generate lower absolute bite forces, and thus require a larger fraction of their maximum bite force to cut the same plant. This article is part of the theme issue 'Food processing and nutritional assimilation in animals'. [ABSTRACT FROM AUTHOR]

Insects use their mandibles for a variety of tasks, including food processing, material transport, nest building, brood care, and fighting. Despite this functional diversity, mandible motion is typically thought to be constrained to rotation about a single fixed axis. Here, we conduct a direct quantitative test of this 'hinge joint hypothesis' in a species that uses its mandibles for a wide range of tasks: Atta vollenweideri leaf-cutter ants. Mandible movements from live restrained ants were reconstructed in three dimensions using a multi-camera rig. Rigid body kinematic analyses revealed strong evidence that mandible movement occupies a kinematic space that requires more than one rotational degree of freedom: at large opening angles, mandible motion is dominated by yaw. But at small opening angles, mandibles both yaw and pitch. The combination of yaw and pitch allows mandibles to 'criss-cross': either mandible can be on top when mandibles are closed. We observed criss-crossing in freely cutting ants, suggesting that it is functionally important. Combined with recent reports on the diversity of joint articulations in other insects, our results show that insect mandible kinematics are more diverse than traditionally assumed, and thus worthy of further detailed investigation. This article is part of the theme issue 'Food processing and nutritional assimilation in animals'. [ABSTRACT FROM AUTHOR]

Background: Immune dysregulation is a feature of sepsis. However, a comprehensive analysis of the immune landscapes in septic patients has not been conducted. Objectives: This study aims to explore the abundance ratios of immune cells in sepsis and investigate their clinical value. Methods: Sepsis transcriptome data sets were downloaded from the NCBI GEO database. The immunedeconv R package was employed to analyze the abundance of immune cells in sepsis patients and calculate the ratios of different immune cell types. Differential analysis of immune cell ratios was performed using the t test. The Spearman rank correlation coefficient was utilized to find the relationships between immune cell abundance and pathways. The prognostic significance of immune cell ratios for patient survival probability was assessed using the log-rank test. In addition, differential gene expression was performed using the limma package, and gene co-expression analysis was executed using the WGCNA package. Results: We found significant changes in immune cell ratios between sepsis patients and healthy controls. Some of these ratios were associated with 28-day survival. Certain pathways showed significant correlations with immune cell ratios. Notably, six immune cell ratios demonstrated discriminative ability for patients with systemic inflammatory response syndrome (SIRS), bacterial sepsis, and viral sepsis, with an Area Under the Curve (AUC) larger than 0.84. Patients with a high eosinophil/B.cell.memory ratio exhibited poor survival outcomes. A total of 774 differential genes were identified in sepsis patients with a high eosinophil/B.cell.memory ratio compared to those with a low ratio. These genes were organized into seven co-expression modules associated with relevant pathways, including interferon signaling, T-cell receptor signaling, and specific granule pathways. Conclusions: Immune cell ratios eosinophil/B.cell.memory and NK.cell.activated/NK.cell.resting in sepsis patients can be utilized for disease subtyping, prognosis, and diagnosis. The proposed cell ratios may have higher prognostic values than the neutrophil-to-lymphocyte ratio (NLR). [ABSTRACT FROM AUTHOR]

In June 2014, the first Health Complex was established in the suburban area of Tabriz, a northwest city of Iran. Health Complexes, as one of the decentralized models, have been formed based on universal health coverage principles. However, nearly two years after commencing work in the province, Health Complexes experience a high turnover of health care workers. This study aims to explore why primary health care workers set aside their roles in the health system and leave their jobs in opposition to the planned objectives. In this qualitative research, purposive and snowball sampling techniques were used for participants' selection. Data were collected through semistructured interviews and documentary review. Interviews were conducted with health officials, health care specialists, and other health workers who had left their jobs in Health Complexes in the suburban areas of Tabriz. Inductive and deductive content analysis was used to analyse data. The leading causes for health care workers leaving a job were divided into four main categories including organizational policies and regulations, financial and economic factors, sociocultural, and personal factors. Developing evidence‐informed interventions and strategies to improve payment systems, provide job security and opportunities for professional development, and adopt cooperative leadership are essential to decrease the turnover rate of health care workers at Health Complexes. Key points: High turnover rates of medical practitioners and medical specialists is the main challenge in suburban areas. Developing evidence‐informed polices is crucial for decreasing turnover rates among clinicians, but such policies are not included among policy makers' planned objectives. Organizational policies and regulations, financial and economic factors, socio‐cultural and personal factors are the leading causes for leaving job by clinicians. [ABSTRACT FROM AUTHOR]

Simple Summary: Three bark beetle species, Tomicus yunnanensis, T. brevipilosus, and T. minor, are the most economically significant pests of the Yunnan pine in Southwestern China. Chemical and physical communication play key roles in various life activities. In this study, we described the fine structure of the adult mouthparts of these three Tomicus species using scanning and transmission electron microscopy. We identified three types of mandibular shapes, which match with their biomechanical properties, their ability to process food, and their preferred foraging locations on tree trunks. Eleven types of sensilla were discernible, including sensilla basiconica, sensilla twig basiconica, sensilla coeloconica, sensilla chaetica, sensilla trichoidea, and sensilla digitiformia. The function roles of each sensilla type were given based on its distribution and structures, especially on the internal structures such as dendrites and tubular body. Most chemoreceptors occur on the palpal tips. No significant differences among the sexes or species were identified; however, intraspecific variability in the number of sensilla twig basiconica 3 and sensilla digitiformia sensilla was evident. These findings will aid future studies of the feeding niches and reproductive behaviors of Tomicus beetles. Tomicus yunnanensis, T. brevipilosus, and T. minor are the most economically significant pests of Pinus yunnanensis in Southwestern China. Chemical and physical factors play critical roles in diverse biological activities. Here, we describe the fine structure of the adult mouthparts of these three Tomicus species using scanning and transmission electron microscopy. We identified three types of mandibular shapes, which determine their biomechanical properties, their ability to process food, and their preferred foraging locations on tree trunks. Eleven types of sensilla were discernible, including sensilla basiconica (Sb.1–2), sensilla twig basiconica (Stb.1–3), sensilla coeloconica (Sco), sensilla chaetica (Sch.1–2), sensilla trichoidea (Str.1–2), and sensilla digitiformia (Sdi). Each basiconic sensillum occurs on the palpal tips and is innervated by 2–6 dendrites. Sb.1 are gustatory receptors, Sb.2 are olfactory receptors, and the three other sensilla have dual taste and mechanical functions. Sco, Sch, and Str are mechanoreceptors. Sdi are mechanical vibration receptions, given that they are innervated by one dendrite with numerous dendritic branches into the nonporous cuticle. No significant differences among the sexes or species were identified; however, intraspecific variability in the number of Stb.3 and Sdi sensilla was evident. These results will aid future studies of Tomicus beetle behaviors. [ABSTRACT FROM AUTHOR]

Aging is characterized by a progressive decline in physiological function and underlies several disabilities, including the increased sensitivity of cells and tissues to undergo pathological oxidative stress. In recent years, efforts have been made to better understand the relationship between age and oxidative stress and further develop therapeutic strategies to minimize the impact of both events on age-related diseases. In this work, we review the impact of the oxidant and antioxidant systems during aging and disease development and discuss the crosstalk of oxidative stress and other aging processes, with a focus on studies conducted in elderly populations., (© 2024 Federation of European Biochemical Societies.)

Deep learning-based computer vision methods are transforming animal behavioural research. Transfer learning has enabled work in non-model species, but still requires hand-annotation of example footage, and is only performant in well-defined conditions. To help overcome these limitations, we developed replicAnt, a configurable pipeline implemented in Unreal Engine 5 and Python, designed to generate large and variable training datasets on consumer-grade hardware. replicAnt places 3D animal models into complex, procedurally generated environments, from which automatically annotated images can be exported. We demonstrate that synthetic data generated with replicAnt can significantly reduce the hand-annotation required to achieve benchmark performance in common applications such as animal detection, tracking, pose-estimation, and semantic segmentation. We also show that it increases the subject-specificity and domain-invariance of the trained networks, thereby conferring robustness. In some applications, replicAnt may even remove the need for hand-annotation altogether. It thus represents a significant step towards porting deep learning-based computer vision tools to the field. Deep learning-based computer vision tools are transforming animal behavioural research; however, many challenges remain. Here, Plum et al. present replicAnt, a novel tool for generating synthetic data to train computer vision models for animal behaviour studies, reducing the need for manual annotation. [ABSTRACT FROM AUTHOR]

Under the conditions of conservation tillage, the existence of the root–soil complex greatly increases the resistance and energy consumption of stubble-cutting blades, especially in Northeast China. In this research, the corn root–soil complex in Northeast China was selected as the research object. Based on the multi-toothed structure of the leaf-cutting ant's mandibles and the unique bite mode of its mandibles on leaves, a gear-tooth, double-disk, bionic stubble-cutting device (BSCD) was developed by using a combination of power cutting and passive cutting. The effects of rotary speed, tillage depth, and forward speed on the torque and power of the BSCD were analyzed using orthogonal tests, and the results showed that all of the factors had a large influence on the torque and power, in the order of tillage depth > rotary speed > forward speed. The performance of the BSCD and the traditional power straight blade (TPSB) was explored using comparative tests. It was found that the optimal stubble-cutting rate of the BSCD was 97.4%. Compared with the TPSB, the torque of the BSCD was reduced by 15.2–16.4%, and the power was reduced by 9.2–11.3%. The excellent performance of the BSCD was due to the multi-toothed structure of the cutting edge and the cutting mode. [ABSTRACT FROM AUTHOR]

The recent global pandemic had shocking impacts on societies and economies worldwide by bringing complexity, ambiguity, and uncertainty for extended periods. UX researchers, faced with numerous challenges, were compelled to adapt to emergent conditions through remote research methods. Investigating how some members of the UX research community adapted to the emergency offers valuable insights into how they responded to uncertainty and showed resilience. Therefore, we studied the early practices of UX managers and researchers in Turkey during the first 6 months of the global pandemic under lockdown conditions. Our goal was to understand how UX research and design teams responded to uncertainty by customizing their methods. We focused on seven firms as different cases to conduct semi-structured interviews with seven managers and 13 UX researchers. The results demonstrate that the adaptable nature of UX research allowed them to quickly respond to these emerging conditions. In the early period of the pandemic, our study participants— UX managers, designers, and researchers—experienced the advantages and challenges of remote UX research necessitated by the lockdown. These included issues related to participant recruitment, work performance, readiness, and methodological concerns. Based on our findings, we make recommendations for UX researchers to conduct moderated and unmoderated remote research while maintaining inclusiveness and rigor. [ABSTRACT FROM AUTHOR]

Aggressive behavior, or lack thereof, is vital to the success of a social insect colony. A diversity of aggressive behaviors are exhibited in varying degrees by workers across ant species. To better understand this suite of behaviors, we review the extensive literature around ant aggression in order to assess the importance of aggression to the success of ant lineages, the ways in which aggression has been quantified in the literature, and potential correlations between aggression and other key functional traits. Our new contributions to this body of literature include an interaction framework for contextualizing the variation of behaviors, a new suggested scale for quantifying aggressive behaviors, and finally an investigation into traits that are correlated with aggression across ant lineages. Based on our phylogenetic comparative analyses, we find a negative correlation between eye length and aggression and that body size, worker polymorphism, and potentially participation in mutualisms with plants and other insects are evolutionarily linked to an increase in aggressive behavior. [ABSTRACT FROM AUTHOR]

Background: Previous studies have confirmed that miR-146a-5p overexpression suppresses neurogenesis, thereby enhancing depression-like behaviors. However, it remains unclear how miR-146a-5p dysregulation produces in vivo brain structural abnormalities in patients with major depressive disorder (MDD). Methods: In this case–control study, we combined cortical morphology analysis of magnetic resonance imaging (MRI) and miR-146a-5p quantification to investigate the neuropathological effect of miR-146a-5p on cortical thickness in MDD patients. Serum-derived exosomes that were considered to readily cross the blood-brain barrier and contain miR-146a-5p were isolated for miRNA quantification. Moreover, follow-up MRI scans were performed in the MDD patients after 6 weeks of antidepressant treatment to further validate the clinical relevance of the relationship between miR-146a-5p and brain structural abnormalities. Results: In total, 113 medication-free MDD patients and 107 matched healthy controls were included. Vertex-vise general linear model revealed miR-146a-5p-dependent cortical thinning in MDD patients compared with healthy individuals, i.e., overexpression of miR-146a-5p was associated with reduced cortical thickness in the left orbitofrontal cortex (OFC), anterior cingulate cortex, bilateral lateral occipital cortices (LOCs), etc. Moreover, this relationship between baseline miR-146a-5p and cortical thinning was nonsignificant for all regions in the patients who had received antidepressant treatment, and higher baseline miR-146a-5p expression was found to be related to greater longitudinal cortical thickening in the left OFC and right LOC. Conclusions: The findings of this study reveal a relationship between miR-146a-5p overexpression and cortical atrophy and thus may help specify the in vivo mediating effect of miR-146a-5p dysregulation on brain structural abnormalities in patients with MDD. [ABSTRACT FROM AUTHOR]

Background: Behvarzs are the backbone of primary healthcare services in the Iranian health system and play a key role in providing efficient, responsive, and equitable services at the first level of service provision. This study aimed to identify the challenges of Behvarzs to provide a perspective for policymakers and managers to help them formulate future programs to enhance the efficiency of the health system. Methods: Following a qualitative design, an inductive content analysis approach was used to analyse the data. The healthcare network of the Alborz province (Iran) was considered as the study context. A total of 27 interviews were conducted with policymakers, development managers, managers of Behavrz training centres, and Behvarz workers in 2020. All interviews were audio-taped and transcribed, followed by data analysis using MAXQDA ver. 10. Results: Five themes were identified: service provision (scope of services, ambiguity of roles, non-compliance with the referral system, the quality of data entry, quality of services), access to equipment (quantity, quality), administrative issues (macro planning, micro planning), training (appropriateness of information, quality of training, recruiting related staff), perceived equity and fairness (balance between workload, income, and benefits, job satisfaction, job promotion). Conclusion: Occupational challenges affect the performance of Behvarzs in responding to society's needs because they not only play a major role in the health system, but also contribute to addressing the communication gap between local communities and high-level institutions, leading to the alignment of policy implementation. Therefore, strategies that emphasise the role of Behvarzs should be followed to promote community engagement. Behvarz workers or community health workers not only play a major role in the health system, but also contribute to filling the gap between communities and high-level institutions by delivering basic healthcare services and for more complicated needs they can refer people to receive appropriate services, leading to more homogeneity in policies. Therefore, strategies are needed to emphasise Behvarz workers' role, with a special focus on engaging local communities. The identified challenges can provide valuable information for health policymakers and the experiences of Behvarz workers can be used to inform international community health workers' programs, which in turn provides better outcomes for treated patients. [ABSTRACT FROM AUTHOR]

Introduction: In Ethiopia, country-wide community-based primary health programs have been in effect for about two decades. Despite the program's significant contribution to advancing Maternal Newborn and Child Health (MNCH), Ethiopia's maternal and child mortality is still one of the highest in the world. The aim of this manuscript is to critically examine the multifaceted fragmentation challenges of Ethiopia's Community Health Workers (CHWs) program to deliver optimum MNCH and build a resilient community health system. Methods: We conducted a qualitative case study in West Shewa Zone, rural Ethiopia. A purposive sampling technique was used to recruit participants. Data sources were two focus group discussions with sixteen CHWs, twelve key informant interviews with multilevel public health policy actors, and a policy document review related to the CHW program to triangulate the findings. Thematic analysis of the qualitative data was conducted. The World Health Organization's health systems framework and socio-ecological model guided the data collection, analysis, and interpretation. Results: The CHWs program has been an extended arm of Ethiopia's primary health system and has contributed to improved health outcomes. However, the program has been facing unique systemic challenges that stem from the fragmentation of health finance; medical and equipment supply; working and living infrastructures; CHWs empowerment and motivation, monitoring, supervision, and information; coordination and governance; and community and stakeholder engagement. The ongoing COVID-19 and volatile political and security issues are exacerbating these fragmentation challenges. Conclusion: This study emphasized the gap between the macro (national) level policy and the challenge during implementation at the micro (district)level. Fragmentation is a blind spot for the community-based health system in rural Ethiopia. We argue that the fragmentation challenges of the community health program are exacerbating the fragility of the health system and fragmentation of MNCH health outcomes. This is a threat to sustain the MNCH outcome gains, the realization of national health goals, and the resilience of the primary health system in rural Ethiopia. We recommend that beyond the current business-as-usual approach, it is important to emphasize an evidence-based and systemic fragmentation monitoring and responsive approach and to better understand the complexity of the community-based health system fragmentation challenges to sustain and achieve better health outcomes. The challenges can be addressed through the adoption of transformative and innovative approaches including capitalizing on multi-stakeholder engagement and health in all policies in the framework of co-production. [ABSTRACT FROM AUTHOR]

Mitochondria are the primary source of energy production and are implicated in a wide range of biological processes in most eukaryotic cells. Skeletal muscle heavily relies on mitochondria for energy supplements. In addition to being a powerhouse, mitochondria evoke many functions in skeletal muscle, including regulating calcium and reactive oxygen species levels. A healthy mitochondria population is necessary for the preservation of skeletal muscle homeostasis, while mitochondria dysregulation is linked to numerous myopathies. In this review, we summarize the recent studies on mitochondria function and quality control in skeletal muscle, focusing mainly on in vivo studies of rodents and human subjects. With an emphasis on the interplay between mitochondrial functions concerning the muscle fiber type-specific phenotypes, we also discuss the effect of aging and exercise on the remodeling of skeletal muscle and mitochondria properties. [ABSTRACT FROM AUTHOR]

Dental topographic analysis has proved a valuable tool for quantifying dental morphology. Established workflows often use proprietary software for pre‐processing dental surfaces, rendering the method expensive and inaccessible to many. This study explores the use of freeware pre‐processing alternatives. We tested 4 decimation tools and 13 smoothing tools across 7 different freeware packages. Surfaces generated via proprietary software could not be replicated, but it was possible to obtain statistically similar measurements using freeware. Based on this investigation, we propose a freeware workflow for researchers to conduct dental topographic analysis, with the expectation that their results will be comparable to that obtained through proprietary methods. [ABSTRACT FROM AUTHOR]

Many social insects display age polyethism: young workers stay inside the nest, and only older workers forage. This behavioural transition is accompanied by genetic and physiological changes, but the mechanistic origin of it remains unclear. To investigate if the mechanical demands on the musculoskeletal system effectively prevent young workers from foraging, we studied the biomechanical development of the bite apparatus in Atta vollenweideri leaf-cutter ants. Fully matured foragers generated peak in vivo bite forces of around 100 mN, more than one order of magnitude in excess of those measured for freshly eclosed callows of the same size. This change in bite force was accompanied by a sixfold increase in the volume of the mandible closer muscle, and by a substantial increase of the flexural rigidity of the head capsule, driven by a significant increase in both average thickness and indentation modulus of the head capsule cuticle. Consequently, callows lack the muscle force capacity required for leaf-cutting, and their head capsule is so compliant that large muscle forces would be likely to cause damaging deformations. On the basis of these results, we speculate that continued biomechanical development post eclosion may be a key factor underlying age polyethism, wherever foraging is associated with substantial mechanical demands. [ABSTRACT FROM AUTHOR]

Local health systems are increasingly tasked to play a more central role in driving action to reduce social inequalities in health. Past experience, however, has demonstrated the challenge of reorienting health system actions towards prevention and the wider determinants of health. In this review, I use meta-ethnographic methods to synthesise findings from eleven qualitative research studies that have examined how ambitions to tackle social inequalities in health take shape within local health systems. The resulting line-of-argument illustrates how such inequalities continue to be problematised in narrow and reductionist ways to fit both with pre-existing conceptions of health, and the institutional practices which shape thinking and action. Instances of health system actors adopting a more social view of inequalities, and taking a more active role in influencing the social and structural determinants of health, were attributed to the beliefs and values of system leaders, and their ability to push-back against dominant discourses and institutional norms. This synthesised account provides an additional layer of understanding about the specific challenges experienced by health workforces when tasked to address this complex and enduring problem, and provides essential insights for understanding the success and shortcomings of future cross-sectoral efforts to tackle social inequalities in health. [ABSTRACT FROM AUTHOR]

A main challenge in the analysis of division of labor in insect societies characterized by worker polymorphism has been identifying the number of physical castes and determining their relationship to task performance. We addressed this question using the extremely polymorphic leafcutter ant Atta cephalotes as a model by measuring 22 morphological characters and applying multivariate Gaussian mixture modeling to define worker size-class boundaries. Our statistical approach discriminated five physical worker size classes (subcastes) and found continuous variation in most morphological characters. Some worker size classes showed patterns of covariance, modularity, and integration in head, mandible, and leg traits, suggesting biomechanical functionality in tasks such as leaf cutting. Task repertoires and act frequencies were recorded to identify how fungal gardening, brood care, leaf-harvesting, and other tasks were distributed across worker size groups. Results showed that small and mid-sized media workers performed more diverse and complex tasks, including leaf harvesting, than workers of other size classes. Minims participated in fungal-gardening and nursing tasks, whereas large medias mainly participated in leafcutting and majors made few direct contributions to fungal cultivation. Results of our integrative analyses demonstrate the existence of an unexpectedly large number of worker size classes that can differ significantly in performance of agricultural tasks. However, overlap in task repertoires of workers at size-class boundaries suggests that not all morphologically distinct groups display discrete behavioral profiles. Morphological groups are instead distinguished by a combination of the tasks that each performs and their relative frequencies. Significance statement: The evolutionary coupling of behavior and morphology is central to understanding division of labor in insect societies. Our work applies robust statistical modelling to offer a novel analysis of worker physical caste categorization based on extensive morphometric sampling. Our method improves upon previous approaches by incorporating measurements of a large number of diverse morphological traits likely involved in task performance to examine worker physical caste evolution. We integrate morphological evolution with behavioral data on relative task performance rates and task repertoire size to describe the morphological and behavioral space of size-variable workers. Our approach provides methods to quantitatively analyze division of labor and behavioral performance, and offers new insights into worker trait modularity and integration and task performance associated with the organization of complex insect societies. [ABSTRACT FROM AUTHOR]

Food processing can exert significant evolutionary pressures on the morphological evolution of animal appendages. The ant genus Pheidole displays a remarkable degree of morphological differentiation and task specialization among its workers. Notably, there is considerable variation in head shape within worker subcastes of Pheidole, which could affect the stress patterns generated by bite‐related muscle contraction. In this study, we use finite element analysis (FEA) to investigate the effect of the variation in head plane shape in stress patterns, while exploring the morphospace of Pheidole worker head shapes. We hypothesize that the plane head shapes of majors are optimized for dealing with stronger bites. Furthermore, we expect that plane head shapes at the edges of each morphospace would exhibit mechanical limitations that prevent further expansion of the occupied morphospace. We vectorized five head shapes for each Pheidole worker type located at the center and edges of the corresponding morphospaces. We conducted linear static FEA to analyze the stresses generated by mandibular closing muscle contraction. Our findings indicate that plane head shapes of majors exhibit signs of optimization to deal with stronger bites. Stresses are distinctly directed along the lateral margins of the head, following the direction of muscle contraction, whereas the stresses on the plane head shapes of minors tend to concentrate around the mandibular articulations. However, the comparatively higher stress levels observed on majors' plane head shapes suggest a demand for cuticular reinforcement, like increased cuticle thickness or sculpturing pattern. Our results align with the expectations regarding the main colony tasks performed by each worker subcaste, and we find evidence of biomechanical limitations on extreme plane head shapes for majors and minors. [ABSTRACT FROM AUTHOR]