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The Medicaid coverage "cliff" occurs when Medicare beneficiaries with household income exceeding 100% of the federal poverty level lose eligibility for supplemental Medicaid coverage. Using a regression discontinuity design with data from Medical Expenditure Panel Survey and National Health and Nutrition Examination Survey for 2007-2019, we demonstrate that the cliff increases out-of-pocket spending by 25% and the probability of experiencing problems paying medical bills by 44.4% without decreases in overall health care spending. However, there is evidence that near-poor Medicare beneficiaries changed behavior in response to the cliff, increasing the use of high-value diagnostic and preventive testing by 8.8% and enrollment in a more affordable plan by 12.2%. The cliff does not encourage healthy behavior., (© 2024 John Wiley & Sons Ltd.)

Using representative data from China, we examine the causal effects of parental retirement on the health of adult children. To do so, we adopt a fuzzy regression discontinuity design and exploit the mandatory retirement ages in China as cut-off points. We find no evidence that parental retirement has significant effects on the mental health, healthcare utilization, or risky health behaviors of adult children. However, paternal retirement and maternal retirement have different effects on adult children's Self-reported health (SRH). Paternal retirement has a significantly negative effect only on the SRH of sons, while maternal retirement does not induce such effects. Potential mechanisms of intergenerational transfer through which parental retirement might affect adult children's health are also explored., (© 2023 The Authors. Health Economics published by John Wiley & Sons Ltd.)

Eucalyptus has become one of the most widely planted species in tropical and subtropical regions in China, with important economic, ecological, and social values. However, it is currently unclear how climate change will affect different Eucalyptus species. Therefore, it is urgent to investigate the potential distribution and dynamics of Eucalyptus under current and future climate scenarios. In this study, we analyzed the potential distribution patterns of the three main Eucalyptus species (Eucalyptus grandis, Eucalyptus urophylla, and Eucalyptus tereticornis) under current and future climatic conditions (2041-2060 and 2061-2080) using the optimized MaxEnt model, which integrates a variety of environmental data including climate, topography, soil, and human influence. We also identified the main factors affecting the potential distributions of the three main Eucalyptus species. Themodel indicated that E. grandis exhibited heightened sensitivity to the mean temperature of the coldest quarter (7.0-20.0 °C) and annual mean temperature (11.9-24.2 °C), whereas E. urophylla displayed heightened sensitivity to precipitation of the warmest quarter (272-1694 mm) and annual precipitation (812-2624mm). Conversely, E. tereticornis demonstrated heightened sensitivity to annual mean temperature (12.7-24.5 °C) and temperature seasonality (63.8-598.9). Under the current climate, E. tereticornis had the widest suitable distribution area (124.91 × 104 km²), followed by E. grandis (124.89 × 104 km²) and E. urophylla (119.81 × 104 km²). Under future climate change scenarios, the suitable ranges of E. grandis, E. urophylla and E. tereticornis will continue to expand. This study highlights the importance of climate change in Eucalyptus distribution and provides quantified potential distribution maps for three Eucalyptus species under current and future climate conditions in China. This research offers valuable scientific insights pertinent to the management and rational site selection for Eucalyptus plantations. [ABSTRACT FROM AUTHOR]

Polycyclic aromatic hydrocarbons (PAHs) are particulate matter bound environmental contaminants known to cause adverse effects on human health. The toxicity of carcinogenic PAH such as benzo[a]pyrene (BaP) has been extensively investigated, whereas other PAHs have received less attention. The present work investigated the toxic effects of three less investigated PAHs with distinct molecular weights in comparison to BaP on co-culture model of human epithelial lung cells (A549) and macrophages (THP-1). Due to the involvement of more than one cell type in the response to PAH exposure, the new co-culture model is considered to be suitable for the prediction of undesired toxicological effects of PAHs. To do so, the co-culture was established and exposed to 0–400 µM of phenanthrene (PHE), fluoranthene (FLA), and, benzo [ghi] perylene (BghiP) for 24 h. Subsequently, cytotoxicity, micronucleus formation, and cytokine excretion were analyzed. The results revealed that the viability of A549 cells decreased after being exposed to increasing concentrations of PAHs. The formation of micronuclei in binucleated cells (BNC) was found more frequently in cells treated with PAHs in comparison to the untreated group, indicating the genotoxic effect of these compounds. Moreover, an exposure to PAHs enhanced the pro-inflammatory cytokine, i.e., interleukin-6 secretion, while diminished the anti-inflammatory cytokine, i.e., interleukin-10. In summary, PAHs possess negative effects on A549 and THP-1 co-culture model, implying an adverse effect on human health when coming into contact with these chemicals via respiration. [ABSTRACT FROM AUTHOR]

Naturally occurring diabetes mellitus (NODM) is one of the most common endocrine disorders in dogs and its etiology closely resembles type 1 diabetes mellitus (T1DM) in people. Human patients with T1DM commonly have cellular derangements consistent with inflammation, impaired immune function, and hypovitaminosis D. There is little information available regarding inflammatory biomarkers, immune function, and vitamin D status in diabetic dogs. Therefore, our objectives were to assess inflammatory biomarkers, vitamin D metabolites, and phagocytic capacity in diabetic dogs and determine whether associations exist with these variables and the level of clinical control or vitamin D metabolites. This was a prospective case--control study that included 20 otherwise healthy diabetic dogs (clinically controlled, n = 10; uncontrolled, n = 10) and 20 nondiabetic, healthy, age (± 2 years), breed, and sex matched controls. Complete blood count, biochemical panel, urinalysis, and fructosamine were performed at a single commercial reference laboratory. Basal plasma tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, and IL-10 were measured using a canine-specific multiplex bead-based assay. Serum C-reactive protein (CRP) was measured using a commercially available ELISA kit. Serum 25-hydroxyvitamin (OH)D3 and 24,25-dihydroxyvitamin (OH)2D3 were measured with HPLC. Phagocytosis of opsonized-Escherichia coli (E. coli) was evaluated with flow cytometry. Diabetic dogs had higher serum CRP concentrations than controls (p = 0.02). Plasma IL-8 concentrations were higher in diabetic dogs with uncontrolled clinical disease compared to controls (p = 0.02). Diabetic dogs had a lower percentage of leukocytes that phagocytized opsonized-E. coli (p = 0.02), but an increased number of bacteria phagocytized per cell (p < 0.001) compared to controls. No between-group differences were identified in vitamin D metabolites, nor were associations found between vitamin D and any variables. Fructosamine had a positive association with serum CRP concentration (rho = 0.35, p = 0.03) and number of bacteria phagocytized per cell (rho = 0.45, p = 0.004) in our cohort (n = 40). Like people with T1DM, diabetic dogs have a proinflammatory phenotype and phagocytic dysregulation that may be correlated with glycemic control. [ABSTRACT FROM AUTHOR]

The economic vibrancy of most small communities is generally driven by Small, Medium and Micro Enterprises (SMMEs) of different kind. Although the existence of these SMMEs tend to depend mainly on the disposition of the entrepreneur, the role of demographic variables tend to be very significant when it comes to their profitability and long-term survival. A myriad of research studies have highlighted the role of demographic characteristics. The paper aims to establish the influence of demographic variables on SMME sustainability and success. We employed a positivist research approach, which is descriptive in nature. A survey was used to collect data from 117 randomly sampled owners/managers respondents from a myriad of SMMEs in the Matjhabeng municipality in Welkom. Yielding a response rate of 69%. A regression analysis and interpretation was conducted to determine the influence of variables to each other. The regression data analysis revealed a mixed-bag of results with (i) education level, (ii) household income and (iii) consumer mobility reporting a significant relationship with SMME sustainability. Conversely, (iv) age level and (v) past work experience showed no significant relationship with SMME sustainability. To ensure sustained SMME growth and success, owner/managers must embrace and entrench a culture of investing not only in their self-development, but that of their team members/workforce as well. The study provides empirical evidence of the positive relationship between demographic variables and SMME's sustained success and survival. Confirming that education and experience in particular are critical predictors of SMME success if investment on the most important asset, people, is given a high priority. [ABSTRACT FROM AUTHOR]

Myelination of axons is a key determinant of fast action potential propagation, axonal health and circuit function. Previously considered a static structure, it is now clear that myelin is dynamically regulated in response to neuronal activity in the central nervous system (CNS). However, how activity-dependent signals are conveyed to oligodendrocytes remains unclear. Here, we review the potential mechanisms by which neurons could communicate changing activity levels tomyelin, with a focus on the accumulating body of evidence to support activity-dependent vesicular signalling directly onto myelin sheaths. We discuss recent in vivo findings of activity-dependent fusion of neurotransmitter vesicles from non-synaptic axonal sites, and how modulation of this vesicular fusion regulates the stability and growth of myelin sheaths. We also consider the potential mechanisms by which myelin could sense and respond to axon-derived signals to initiate remodelling, and the relevance of these adaptations for circuit function. We propose that axonal vesicular signalling represents an important and underappreciated mode of communication by which neurons can transmit activity-regulated signals to myelinating oligodendrocytes and, potentially, more broadly to other cell types in the CNS. [ABSTRACT FROM AUTHOR]

Regression discontinuity (RD) designs have gained significant popularity as a quasi-experimental device for evaluating education programs and policies. In this paper, we present a comprehensive review of RD designs, focusing on the continuity-based framework, the most widely adopted RD framework. We first review the fundamental aspects of RD designs, drawing on potential outcomes and causal graphs. We then discuss the validity threats in RD designs, including manipulation, discreteness of the running variable, statistical power, and generalizability. Additionally, we provide an overview of the existing extensions to RD designs. To exemplify the application of RD methods, we analyze the effect of New Jersey's pre-kindergarten program on children's vocabulary test scores, using an educational dataset. Finally, we offer practical guidelines in the conclusion to promote the appropriate use of RD methods in educational research. [ABSTRACT FROM AUTHOR]

Damming can promote flood control, water supply, power generation, and shipping but often changes the downstream hydrological regimes, producing adverse externality effects. Maximizing downstream social and ecological benefits will inevitably reduce upstream power generation. This study presents two novel bi-level hybrid game models, called the non-cooperative hybrid game model (NCHG) and the cooperative hybrid game model (CHG), to facilitate integrated water resource management in reservoir systems. The performance index of propensity to disrupt is applied to evaluate the stability of CHG, and an improved reliability index and Gini coefficient are adopted to evaluate the reliability and equity of both two models. The Three Gorges Reservoir and its adjacent cities were chosen as a case to inspect the two models' performance. A range of scheduling schemes was derived by proposed bi-level hybrid game models in wet, normal, and dry years. Results reveal that (i) the RI values of the watershed system obtained by the CHG are less than those in NCHG in three typical years (for example, 0.1201 VS 0.1930 in the wet year), showing higher systemic reliability. The Gini coefficients of the watershed system obtained by the CHG are all less than those obtained by the NCHG in all typical years (for example, 0.1016 VS 0.1020 in the wet year), which shows better performance of CHG on fairness for the allocation results; (ii) in the case of a multi-function reservoir system, the CHG generates favorable allocation schemes with higher systemic characteristic values by 32.43, 34.39, and 33.54 in wet, normal, and dry years, respectively, than those in NCHG (32.03, 33.16, and 31.42 in wet, normal, and dry years, respectively); (iii) compared with NCHG, the economic benefits obtained by CHG decreased by 0.98%, 1.04%, and 5.42% in wet, normal, and dry years, respectively; the social negative benefits decreased by 3.49%, 9.84%, and 28.69%; and the ecological negative benefits decreased by 1.77%, 5.65%, and 5.59%, respectively. It indicates that a minor sacrifice of the reservoir benefit could significantly improve the welfare at the system level by the CHG. The developed CHG can provide optimal water scheduling schemes in balancing inter-regional water conflicts and can be widely used to produce an equilibrium management strategy for a multi-function reservoir system. [ABSTRACT FROM AUTHOR]

This paper examines whether state and local political leaders' partisan affiliations affect economic freedom at the metropolitan statistical area (MSA) level. Because political leaders' partisan affiliations likely correlate with unobserved state- and local-level characteristics that also affect the economic freedom levels of MSAs, we use a regression discontinuity design that leverages close elections as natural experiments to identify a causal effect. Using Stansel (2019)'s index, which aggregates a bundle of policies into a single measure of economic freedom, we find that close Republican majorities in the lower State House increase overall MSA-level economic freedom. This effect is realized through consistent governments (i.e., Republican governor and Republican State House majority) and primarily through changes in labor market policy. At the city level, Republican mayors also increase overall MSA-level economic freedom levels. [ABSTRACT FROM AUTHOR]

State government's role in monitoring local governments' fiscal distress gained importance after several local financial crises. Although many states have implemented state monitoring systems, the effectiveness of these systems has not been well understood. Using the case of the New York State, we conducted a regression discontinuity analysis to examine the effect of fiscal stress labeling on local governments. We find some evidence to support that fiscal stress labels lead local governments to improve their fund balance and cash positions. Specifically, school districts at the early stage of fiscal stress are more responsive to the labels than districts under more severe fiscal stress. [ABSTRACT FROM AUTHOR]

Salinity stress severely restricts rice growth. Prohexadione calcium (Pro-Ca) modulation can effectively alleviate salt stress in rice. In this study, we explored the effects of Pro-Ca on enhancing salt tolerance in two rice varieties, IR29 and HD96-1. The results revealed that Pro-Ca markedly enhanced root and shoot morphological traits and improved plant biomass under salt stress. Chlorophyll a and b content were significantly increased, which improved photosynthetic capacity. Transcriptomic and metabolomic data showed that Pro-Ca significantly up-regulated the expression of genes involved in E3 ubiquitin ligases in IR29 and HD96-1 by 2.5-fold and 3-fold, respectively, thereby maintaining Na+ and K+ homeostasis by reducing Na+. Moreover, Pro-Ca treatment significantly down-regulated the expression of Lhcb1, Lhcb2, Lhcb3, Lhcb5, and Lhcb6 in IR29 under salt stress, which led to an increase in photosynthetic efficiency. Furthermore, salt stress + Pro-Ca significantly increased the A-AAR of IR29 and HD96-1 by 2.9-fold and 2.5-fold, respectively, and inhibited endogenous cytokinin synthesis and signal transduction, which promoted root growth. The current findings suggested that Pro-Ca effectively alleviated the harmful effects of salt stress on rice by maintaining abscisic acid content and by promoting oxylipin synthesis. This study provides a molecular basis for Pro-Ca to alleviate salt stress in rice. [ABSTRACT FROM AUTHOR]

Coinfection with Mycobacterium tuberculosis (Mtb) and the human immunodeficiency virus (HIV) is a significant public health concern. Individuals infected with Mtb who acquire HIV are approximately 16 times more likely to develop active tuberculosis. T cells play an important role as both targets for HIV infection and mediators of the immune response against both pathogens. This review aims to synthesize the current literature and provide insights into the effects of HIV/Mtb coinfection on T cell populations and their contributions to immunity. Evidence from multiple in vitro and in vivo studies demonstrates that T helper responses are severely compromised during coinfection, leading to impaired cytotoxic responses. Moreover, HIV's targeting of Mtb-specific cells, including those within granulomas, offers an explanation for the severe progression of the disease. Herein, we discuss the patterns of differentiation, exhaustion, and transcriptomic changes in T cells during coinfection, as well as the metabolic adaptations that are necessary for T cell maintenance and functionality. This review highlights the interconnectedness of the immune response and the pathogenesis of HIV/Mtb coinfection. [ABSTRACT FROM AUTHOR]

Purpose: The study's objective is to assess the environmental performance of rice production in Northern Italy, in particular in Piedmont, the first Italian and European district for the rice-growing area, and thus identify the most critical hotspots and agricultural processes. In particular, as a case study, a farm located in Vercelli (VC) has been chosen. Subsequently, the study results were compared with other different cultivation practices to evaluate the most sustainable choice. Methods: The application of the LCA has been performed, highlighting the phases of rice production that have the most significant impact. Then, uncertainty and sensitivity analyses have been made to estimate the robustness of the results and assess the influence of changing some input variables on emission reduction. Finally, multivariate statistical, specifically a principal component analysis (PCA), was conducted to aid the interpretation of the output dataset of this case study. LCA, uncertainty analysis, and sensitivity analysis were performed with SimaPro 9.2.0, using ReCiPe 2016 Midpoint (H) methodology, and PCA with R software. Results and discussions: The hotspot with the highest environmental load is irrigation, which compared to the other phases impacts more in 15 out of 18 categories, including 12 with impacts greater than + 75%. This is because irrigation causes direct impacts, related to the methanogenesis in rice fields, but also indirect impacts related mainly to the production of the energy mix required to move the large masses of irrigation water. Therefore, different water management systems were compared and results show that the irrigation systems based on intermittent paddy submergence (DSI) could result in − 40% lower impacts, resulting to be the preferable technique over the other irrigation systems analyzed, including the traditional one used in this study. Conclusions: In order to reduce the environmental impacts related to the irrigation process, a water management system characterized by intermittent flooding of the paddy field (DSI) could be used as it reduces the environmental impacts the most (− 40%), while the least suitable system is one characterized by continuous flooding without drought periods, as it causes the highest impacts. [ABSTRACT FROM AUTHOR]

Introduction: The COVID-19 pandemic has led to approximately 3.5 million cases in Romania, causing systemic inflammation and over 200 symptoms affecting various body systems. This complexity has challenged rehabilitation systems, necessitating personalized plans tailored to each patient's illness stage and impairment level. The ISPRM-developed ClinFIT COVID-19 instrument, aligned with the ICF categories, assists in assessing patients during acute, post-acute, and long-term phases. Objective: This study aimed to evaluate and assess functional impairments in post-COVID-19 patients in Romania, with a secondary goal of generating rehabilitation directions. Methods: Data were collected from patients at two Bucharest medical centers, including those with persistent symptoms post-acute phase. Participants were assessed using the adapted ClinFIT COVID-19 instrument, and descriptive statistics were applied. Conclusions: Findings revealed diverse functional impairments in physical, psychological, and social domains among post-COVID-19 patients, with severe impairments more common in those with long-term COVID-19. Complete impairment in complex movement and paid work was noted, affecting one-third of salaried employees and forcing some to retire. In the acute phase, the most frequent functional impairments were sleep, attention, pain sensation, and exercise tolerance functions. In contrast, the most severely affected functions were exercise tolerance and mobility joint functions. Age did not positively correlate with any of the analyzed functions. In the post-acute phase, sleep, energy, and drive functions remained the most frequently affected functions, while the most severely affected was, by far, the moving around function. In the post-acute period, respiratory and respiratory muscle functions strongly correlated with all tasks related to physical activity. In the long COVID-19 phase, remunerative employment was the most severely affected function, while attention functions remained the most frequently affected, similar to the acute phase. The ClinFIT COVID-19 instrument effectively captured these impairments, underscoring the need for comprehensive rehabilitation strategies. [ABSTRACT FROM AUTHOR]

The histochemical Falck-Hillarp method for the localization of dopamine (DA), noradrenaline (NA) and serotonin in the central nervous system (CNS) of rodents was introduced in the 1960s. It supported the existence of chemical neurotransmission in the CNS. The monoamine neurons in the lower brain stem formed monosynaptic ascending systems to the telencephalon and diencephalon and monoamine descending systems to the entire spinal cord. The monoamines were early on suggested to operate via synaptic chemical transmission in the CNS. This chemical transmission reduced the impact of electrical transmission. In 1969 and the 1970s indications were obtained that important modes of chemical monoamine communication in the CNS also took place through the extra-synaptic fluid, the extracellular fluid, and longdistance communication in the cerebrospinal fluid involving diffusion and flow of transmitters like DA, NA and serotonin. In 1986, this type of transmission was named volume transmission (VT) by Agnati and Fuxe and their colleagues, also characterized by transmitter varicosity and receptor mismatches. The short and long-distance VT pathways were characterized by volume fraction, tortuosity and clearance. Electrical transmission also exists in the mammalian CNS, but chemical transmission is in dominance. One electrical mode is represented by electrical synapses formed by gap junctions which represent low resistant passages between nerve cells. It allows for a more rapid passage of action potentials between nerve cells compared to chemical transmission. The second mode is based on the ability of synaptic currents to generate electrical fields to modulate chemical transmission. One aim is to understand how chemical transmission can be integrated with electrical transmission and how putative (aquaporin water channel, dopamine D2R and adenosine A2AR) complexes in astrocytes can significancy participate in the clearance of waste products from the glymphatic system. VT may also help accomplish the operation of the acupuncture meridians essential for Chinese medicine in view of the indicated existence of extracellular VT pathways. [ABSTRACT FROM AUTHOR]

Introduction. Triple‐negative breast cancer (TNBC) is a subgroup of breast cancer characterized by the absence of estrogen and the human epidermal 2 receptor and also a lack of targeted therapy options. Chemotherapy has so far been the only approved treatment option, and patients with metastatic cancer have a dismal prognosis with a median overall survival (OS) of approximately 14 months. Identification of druggable targets for metastatic TNBC is therefore of special interest. Methods. A systematic search was performed, to review the existing evidence on targeted therapies in metastatic TNBC. Results. A total of 37 phase 2/3 studies were identified, evaluating 29 different targeted agents. In this review, results on progression free survival (PFS) and OS are presented. Conclusion. In most of the studies included, no improvement was observed for neither PFS nor OS; however, a few studies did show improvement with targeted agents and have led to new treatment options in subgroups of patients. The antibody drug conjugate, sacituzumab govitecan, demonstrated superior PFS and OS in comparison to chemotherapy. Immunotherapy with checkpoint inhibitors such as atezolizumab and pembrolizumab is now recommended as a first‐line treatment option for patients with expression a PD‐L1 positive tumor. Finally, the poly adenosine diphosphate‐ribose polymerase (PARP) inhibitors talazoparib and olaparib are recommended, as first‐line treatment options in patients with metastatic breast cancer and a germline BRCA mutation, but an immune checkpoint inhibitor should be considered for the subset of these patients who are PD‐L1 positive. [ABSTRACT FROM AUTHOR]

The salt-tolerant marine microalgae Dunaliella tertiolecta is reported to generate significant amounts of intracellular glycerol as an osmoprotectant under high salt conditions. This study highlights the phylogenetic distribution and comparative glycerol biosynthesis of seven new Dunaliella isolates compared to a D. tertiolecta reference strain. Phylogenetic analysis indicates that all Dunaliella isolates are newly discovered and do not relate to the D. tertiolecta reference. Several studies have identified light color and intensity and salt concentration alone as the most inducing factors impacting glycerol productivity. This study aims to optimize glycerol production by investigating these described factors singularly and in combination to improve the glycerol product titer. Glycerol production data indicate that cultivation with white light of an intensity between 500 and 2000 μmol m−2 s−1 as opposed to 100 μmol m−2 s−1 achieves higher biomass and thereby higher glycerol titers for all our tested Dunaliella strains. Moreover, applying higher light intensity in a cultivation of 1.5 M NaCl and an increase to 3 M NaCl resulted in hyperosmotic stress conditions, providing the highest glycerol titer. Under these optimal light intensity and salt conditions, the glycerol titer of D. tertiolecta could be doubled to 0.79 mg mL−1 in comparison to 100 μmol m−2 s−1 and salt stress to 2 M NaCl, and was higher compared to singularly optimized conditions. Furthermore, under the same conditions, glycerol extracts from new Dunaliella isolates did provide up to 0.94 mg mL−1. This highly pure algae-glycerol obtained under optimal production conditions can find widespread applications, e.g., in the pharmaceutical industry or the production of sustainable carbon fibers. [ABSTRACT FROM AUTHOR]

Limited research has been carried out on Indigenous young people transitioning out of care in Canada. This article provides a coherent and meaningful account of the journey of aging out of care in the province of Manitoba, as presented by 17 Indigenous care leavers. Using a qualitative multilevel approach grounded in first-person narratives, this study focused on incorporating and elevating the voices of Indigenous care leavers. Participants detailed their experiences of planning for the transition and aging out of care, and described their life post-care. The care leavers expressed that the Child and Family Services-led process of planning for and transitioning out of care was unclear and failed to engage them as partners, and as a result left them ill-prepared for life post-care. Additionally, participants’ experiences serve as evidence of enduring gaps in transition supports, namely in mental health and interpersonal supports. These supports were often unavailable or inaccessible, challenges that were amplified during participants’ transition out of care and in the post-care period. The experiential knowledge from this study contributes valuable information to inform responsive child welfare transition policies and practices that are reflective of the experiences and needs of the population it intends to serve. [ABSTRACT FROM AUTHOR]

This research investigates the effects of a success coaching program by examining the timing of the first appointment and the frequency of additional coaching. Using a fuzzy regression discontinuity approach, we provide Local Average Treatment Effects of the program when students make appointments early in the semester. Students with appointments scheduled before discontinuity had an average of two additional meetings compared to students who waited to make their first appointment. We find each coaching session yields an effect of about 0.2–0.3 grade points and approximately 0.8 additional credits earned. This article contributes to understanding of the timing and frequency effects for success coaching and recommends programs aim to have first interactions with students happen before the sixth week of classes and offer four to six appointments per semester for maximum impact. [ABSTRACT FROM AUTHOR]

The operation of hydropower plants leads to sudden changes in river stage and to a flow regime known as hydropeaking. Hydropeaking alters the morphology of the riverbed and water quality, and ultimately poses a risk to riverine ecosystems. While many indicators are available to quantitatively assess this problem in rivers, the impact of hydropeaking on aquifers is largely unknown and lacks of quantitative indicators. We analyze with wavelet techniques the spatial and temporal dynamics of surface water‐groundwater interaction in an aquifer impacted by two differently regulated rivers. We propose four indicators to study the aquifer stress produced by hydropeaking and classify the observed groundwater head time series into weakly, moderately and highly impacted. This study opens the possibility for a quantitative assessment of the impact of hydropeaking on the groundwater ecosystem. Plain Language Summary: The amount of water that hydropower plants release to the river downstream depends on the energy demand. This can lead to fast and frequent changes in the water level of the rivers, a regime also known as hydropeaking. Hydropeaking affects the river morphology, the water quality and the river ecosystem, and there are many indicators to assess these problems. On the contrary, the effects on aquifers are less studied. In this work, we analyze how rivers influence the groundwater table in a region affected by hydropeaking. Additionally, we propose different indicators to evaluate the degree of stress in the aquifer at different locations. We expect that our indicators can be used in the future to assess the impact of hydropeaking on the groundwater ecosystems. Key Points: Four indicators are proposed to assess the impact of hydropeaking on aquifersAquifer stress indicators capture different aspects of hydropeaking propagation in groundwaterGroundwater impact should be included in future ecohydrological assessments [ABSTRACT FROM AUTHOR]

Introduction: Individuals recovering from COVID-19 often experience a range of post-recovery symptoms. However, the literature on post-COVID-19 symptoms reveals conflicting results, necessitating a heightened focus on longitudinal studies to comprehend the trajectory of impairments over time. Our study aimed to investigate changes in long-term impairments among individuals infected with COVID-19 and explore potential predictors influencing these changes. Methods: We conducted a web-survey targeting individuals that had been infected with COVID-19 at four time-points: T0 (baseline), T1 (three months), T2 (six months), and T3 (twelve months). The survey included contextual factors, factors related to body functions and structures, and post-COVID impairments. The longitudinal sample included 213 individuals (with a mean age of 48.92years). Linear mixed models were employed to analyze changes in post-COVID impairments over time and identify impacting factors. Results: Findings revealed a general decline in post-COVID impairments over time, with each symptom exhibiting a dynamic pattern of fluctuations. Factors such as initial infection severity, education level, and work status were significantly associated with the levels of impairments. Discussion: The study emphasizes that post-COVID impairments are not static but exhibit variations over time. Personalized care, especially for vulnerable populations, is crucial. The results underscore the need for long-term monitoring and multidisciplinary treatment approaches. Targeted support and interventions are highlighted for individuals with severe initial infections and those in socioeconomically disadvantaged groups. [ABSTRACT FROM AUTHOR]

Oligodendrocyte progenitor cells (OPCs) represent a subtype of glia, giving rise to oligodendrocytes, the myelin-forming cells in the central nervous system (CNS). While OPCs are highly proliferative during development, they become relatively quiescent during adulthood, when their fate is strictly influenced by the extracellular context. In traumatic injuries and chronic neurodegenerative conditions, including those of autoimmune origin, oligodendrocytes undergo apoptosis, and demyelination starts. Adult OPCs become immediately activated; they migrate at the lesion site and proliferate to replenish the damaged area, but their efficiency is hampered by the presence of a glial scar—a barrier mainly formed by reactive astrocytes, microglia and the deposition of inhibitory extracellular matrix components. If, on the one hand, a glial scar limits the lesion spreading, it also blocks tissue regeneration. Therapeutic strategies aimed at reducing astrocyte or microglia activation and shifting them toward a neuroprotective phenotype have been proposed, whereas the role of OPCs has been largely overlooked. In this review, we have considered the glial scar from the perspective of OPCs, analysing their behaviour when lesions originate and exploring the potential therapies aimed at sustaining OPCs to efficiently differentiate and promote remyelination. [ABSTRACT FROM AUTHOR]

The primary research question driving this paper is the following: "What are the schooling experiences of young people who are at risk of or experiencing homelessness?" Through interviews with 28 young people in two cities in Ontario, Canada, the authors identified several common experiences, including the following: lack of available information that could help them cope with their housing difficulties; prejudice and bullying from other students, sometimes stemming from their housing problems but also due to factors such as racialization, gender identity, poverty, and substance use; and individual support from some teachers and support staff, although this was dependent on being in school. They proposed changes to help young people still in school, including the inclusion of non-judgmental information and guidance on dealing with poverty and homelessness in school curricula, educating school staff about the 'symptoms' of homelessness to help them identify students at risk, and creating more safe and supportive school environments overall. [ABSTRACT FROM AUTHOR]

Alzheimer's disease (AD) is a progressive neurodegenerative disease with no effective treatments, not least due to the lack of authentic animal models. Typically, rodent models recapitulate the effects but not causes of AD, such as cholinergic neuron loss: lesioning of cholinergic neurons mimics the cognitive decline reminiscent of AD but not its neuropathology. Alternative models rely on the overexpression of genes associated with familial AD, such as amyloid precursor protein, or have genetically amplified expression of mutant tau. Yet transgenic rodent models poorly replicate the neuropathogenesis and protein overexpression patterns of sporadic AD. Seeding rodents with amyloid or tau facilitates the formation of these pathologies but cannot account for their initial accumulation. Intracerebral infusion of proinflammatory agents offer an alternative model, but these fail to replicate the cause of AD. A novel model is therefore needed, perhaps similar to those used for Parkinson's disease, namely adult wildtype rodents with neuron-specific (dopaminergic) lesions within the same vulnerable brainstem nuclei, 'the isodendritic core', which are the first to degenerate in AD. Site-selective targeting of these nuclei in adult rodents may recapitulate the initial neurodegenerative processes in AD to faithfully mimic its pathogenesis and progression, ultimately leading to presymptomatic biomarkers and preventative therapies. [ABSTRACT FROM AUTHOR]

Traumatic spinal cord injury (SCI) is a life-threatening and life-altering condition that results in debilitating sensorimotor and autonomic impairments. Despite significant advances in the clinical management of traumatic SCI, many patients continue to suffer due to a lack of effective therapies. The initial mechanical injury to the spinal cord results in a series of secondary molecular processes and intracellular signaling cascades in immune, vascular, glial, and neuronal cell populations, which further damage the injured spinal cord. These intracellular cascades present promising translationally relevant targets for therapeutic intervention due to their high ubiquity and conservation across eukaryotic evolution. To date, many therapeutics have shown either direct or indirect involvement of these pathways in improving recovery after SCI. However, the complex, multifaceted, and heterogeneous nature of traumatic SCI requires better elucidation of the underlying secondary intracellular signaling cascades to minimize off-target effects and maximize effectiveness. Recent advances in transcriptional and molecular neuroscience provide a closer characterization of these pathways in the injured spinal cord. This narrative review article aims to survey the MAPK, PI3K-AKT-mTOR, Rho-ROCK, NF-κB, and JAK-STAT signaling cascades, in addition to providing a comprehensive overview of the involvement and therapeutic potential of these secondary intracellular pathways following traumatic SCI. [ABSTRACT FROM AUTHOR]

Amphiphilic silk fibroin (SF) forms stable adsorption layers at the air–water interface. The range of the investigated protein concentrations can be divided into two parts according to the peculiarities of the surface layer properties. At protein concentrations from 0.0005 to 0.01 mg/mL, the dynamic surface elasticity monotonically increases with the concentration and surface age and reaches values of up to 220 mN/m. In this range, the adsorption layer compression leads to a fast increase of the surface pressure. In the second part (>0.01 mg/mL), the surface elasticity decreases again and the kinetic dependences of the film thickness and adsorbed amount change only a little. In this case, the layer compression leads only to a slight increase of the surface pressure. These two types of behavior can be attributed to the distinctions in the protein aggregation in the surface layer. Atomic force microscopy (AFM) investigations of the layers transferred from the liquid surface onto a mica surface by the Langmuir–Schaefer method show some peculiarities of the layer morphology in the intermediate concentration range (~0.02 mg/mL). [ABSTRACT FROM AUTHOR]

Progesterone receptor membrane component 1 (PGRMC1) is one of few proteins that have been recently described as direct modulators of the activity of human cytochrome P450 enzymes (CYP)s. These enzymes form a superfamily of membrane-bound hemoproteins that metabolize a wide variety of physiological, dietary, environmental, and pharmacological compounds. Modulation of CYP activity impacts the detoxification of xenobiotics as well as endogenous pathways such as steroid and fatty acid metabolism, thus playing a central role in homeostasis. This review is focused on nine main topics that include the most relevant aspects of past and current PGRMC1 research, focusing on its role in CYP-mediated drug metabolism. Firstly, a general overview of the main aspects of xenobiotic metabolism is presented (I), followed by an overview of the role of the CYP enzymatic complex (IIa), a section on human disorders associated with defects in CYP enzyme complex activity (IIb), and a brief account of cytochrome b5 (cyt b5)'s effect on CYP activity (IIc). Subsequently, we present a background overview of the history of the molecular characterization of PGRMC1 (III), regarding its structure, expression, and intracellular location (IIIa), and its heme-binding capability and dimerization (IIIb). The next section reflects the different effects PGRMC1 may have on CYP activity (IV), presenting a description of studies on the direct effects on CYP activity (IVa), and a summary of pathways in which PGRMC1's involvement may indirectly affect CYP activity (IVb). The last section of the review is focused on the current challenges of research on the effect of PGRMC1 on CYP activity (V), presenting some future perspectives of research in the field (VI). [ABSTRACT FROM AUTHOR]