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This article presents results of research This article presents an excerpt from a doctoral thesis that researched on the manner of organizing Chemistry teacher training courses in the State of Minas Gerais, based on the formative dimensions necessary for professional development and teacher’s work. In this text, we focus on the 'basic and disciplinary conceptual dimension' and the 'instrumental, technical and practical dimension' in order to outline the profile of the Chemistry Licentiate Degree courses studied and making inferences about the possibilities and training needs that encompass in the construction of teacher’s professional identity. Therefore, we analyzed the pedagogical projects of the Chemistry Licentiate Degree course of three federal public universities of the mentioned State with regard to the organization of their pedagogical proposals and their curricular matrices. We identified that one of the courses tends to offer teacher education with bachelor's degree characteristics, reverberating in restrictions to the construction of the teaching identity. The other two courses, despite the need for some adjustments in their pedagogical proposals, provide for the constitution of the Chemistry teacher’s professional identity.

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Bioquímica. Fecha de lectura: 15-09-2021, La forma genética más prevalente en la cardiomiopatía arritmogénica (ACM, del inglés “arrhythmogenic cardiomyopathy”) es causada por mutaciones en la placofilina-2 (PKP2). La mayoría de las mutaciones en PKP2 descritas en pacientes con ACM, generan proteínas truncadas. Sin embargo, los mecanismos a través de los cuales estas mutaciones en PKP2 provocan el desarrollo de la ACM, son poco conocidos. El objetivo de este estudio fue investigar los mecanismos moleculares subyacentes al desarrollo de un fenotipo estructural de ACM, inducido por mutaciones que provocan la delección de la región C-terminal de PKP2 (CT) e identificar posibles dianas terapéuticas. En este trabajo se caracterizó el efecto de la proteína mutante de PKP2 con delección de la región CT, R735X. Para ello, mediante el uso de imagen por resonancia magnética cardíaca (MRI), se observó que la función cardíaca del ventrículo derecho (VD) estaba comprometida en ratones transducidos con AAV-R735X. Además, combinando diversas técnicas como microscopía confocal, microscopio de fuerza atómica (AFM) o transferencia de energía de resonancia de Föster (FRET), se observó que la presencia del mutante R735X, alteraba la distribución de los componentes del citoesqueleto de actomiosina, su estructura y las propiedades de los filamentos, afectando a la arquitectura celular. Este trabajo revela que PKP2 es capaz de influir en la regulación del citoesqueleto de actina a través de la modulación de la actividad de la MYH10, lo que sugiere que la proteína mutante R735X actúa como un mutante con ganancia de función El modelo tridimensional de la estructura de PKP2, reveló que la región CT era necesaria para estabilizar la estructura de la proteína. Por tanto, la proteína mutante R735X sufría un cambio conformacional y exponía una región interna que en la estructura de PKP2 “wild-type” quedaba oculta, alterando su función, su localización y su interacción con otras proteínas. La oclusión estérica de esta región interna, en la proteína R735X-Ctag, condujo a una recuperación parcial del fenotipo estructural que provocaba el mutante R735X, indicando que puede ser una nueva diana terapéutica en la ACM

Two strategies based on multivariate qualitative and quantitative analysis are proposed for determining whether paprika samples are adulterated with Sudan I or II dyes. The samples were characterized by their UV–visible spectrum. Then, the following chemometric techniques were applied: principal component analysis (PCA) for the data exploration stage, partial least squares-discriminant analysis (PLS-DA) for the qualitative strategy and partial least squares (PLS) for the Sudan quantification strategy. Three classes were considered in the qualitative analysis: unadulterated, adulterated with Sudan I and adulterated with Sudan II. For the quantitative analysis, models were developed for each adulterant in a concentration range of 0–12 mg g−1, where 0 mg g−1 stands for the unadulterated samples. Both strategies gave satisfactory results. The multi-class PLS-DA model properly differentiate between the three types of studied samples: non-adulterated and adulterated with Sudan I and with Sudan II. Only samples at the lowest concentration (1 mg g−1) were wrongly detected as unadulterated. The PLS models were validated by setting the regression line of the model-predicted concentration versus the real concentration for the test set samples. The slope and intercept of each regression line were compared with those of the ideal line (0, 1) by means of the joint test of ordinate and slope.

We present a novel metric for measuring relative connection between parts of a city using geotagged Twitter data as a proxy for co-occurrence of city residents. We find that socioeconomic similarity is a significant predictor of this connectivity metric, which we call “linkage strength”: neighborhoods that are similar to one another in terms of residents’ median income, education level, and (to a lesser extent) immigration history are more strongly connected in terms of the of people who spend time there, indicating some level of homophily in the way that individuals choose to move throughout a city’s districts. The authors would like to acknowledge the Senseable Stockholm Lab and its partners: City of Stockholm, KTH Royal Institute of Technology, the Stockholm Chamber of Commerce and Newsec, in collaboration with MIT Senseable City Lab. We would also like to acknowledge the H2020 5G-TOURS project. Finally, we would like to thank the City of Stockholm municipality's Statistical Information Service for provision of population counts and socioeconomic data.

Proceeding of: 2020 Mediterranean Communication and Computer Networking Conference (MedComNet), Aroa, Italy, 17-19 June 2020 In this paper, we investigate the existence and prevalence of comparable dynamics in the temporal fluctuations for the traffic demands generated by mobile applications.To this end, we hinge upon a spectral analysis framework, by computing Discrete Fourier Transforms of the typical demands for tens of popular mobile services observed in an operational metropolitan-scale network. We filter, cluster, and analyse hundreds of frequency components, and identify a substantial set of regular patterns that are common across most service demands. We also unveil how several mobile services defy classification, and have instead highly distinguishing temporal dynamics. The work of Orange Labs was supported by ANR through the CANCAN project (ANR-18-CE25-0011). The work of UC3M was supported by the H2020 5G-TOURS project (grant agreement no. 856950).

The economic sustainability of future mobile networks will largely depend on the strong specialization of its offered services. Network operators will need to provide added value to their tenants, by moving from the traditional one-size-fits-all strategy to a set of virtual end-to-end instances of a common physical infrastructure, named network slices , which are especially tailored to the requirements of each application. Implementing network slicing has significant consequences in terms of resource management: service customization entails assigning to each slice fully dedicated resources, which may also be dynamically reassigned and overbooked in order to increase the cost-efficiency of the system. In this paper, we adopt a data-driven approach to quantify the efficiency of resource sharing in future sliced networks. Building on metropolitan-scale real-world traffic measurements, we carry out an extensive parametric analysis that highlights how diverse performance guarantees, technological settings, and slice configurations impact the resource utilization at different levels of the infrastructure in presence of network slicing. Our results provide insights on the achievable efficiency of network slicing architectures, their dimensioning, and their interplay with resource management algorithms at different locations and reconfiguration timescales. The work of University Carlos III of Madrid was supported by the H2020 5G-MoNArch project (Grant Agreement No. 761445) and the work of NEC Laboratories Europe by the 5GTransformer project (Grant Agreement No. 761536). The work of CNR-IEIIT was partially supported by the ANR CANCAN project (ANR-18-CE25-0011). Publicado

Proceeding of: MobiCom '18: The 24th Annual International Conference on Mobile Computing and Networking, New Delhi, India, October 29 - November 2, 2018 By providing especially tailored instances of a virtual network,network slicing allows for a strong specialization of the offered services on the same shared infrastructure. Network slicing has profound implications on resource management, as it entails an inherent trade-off between: (i) the need for fully dedicated resources to support service customization, and (ii) the dynamic resource sharing among services to increase resource efficiency and cost-effectiveness of the system. In this paper, we provide a first investigation of this trade-off via an empirical study of resource management efficiency in network slicing. Building on substantial measurement data collected in an operational mobile network (i) we quantify the efficiency gap introduced by non-reconfigurable allocation strategies of different kinds of resources, from radio access to the core of the network, and (ii) we quantify the advantages of their dynamic orchestration at different timescales. Our results provide insights on the achievable efficiency of network slicing architectures, their dimensioning, and their interplay with resource management algorithms. We would like to thank the shepherd and reviewers for their valuable comments and feedback. The work of University Carlos III of Madrid was supported by the H2020 5G-MoNArch project (grant agreement no. 761445), and the work of NEC Europe Ltd. was supported by the H2020 5GTransformer project (grant agreement no. 761536).

Proceeding of: 13th International Conference on emerging Networking EXperiments and Technologies (CoNEXT '17) We investigate how individual mobile services are consumed at a national scale, by studying data collected in a 3G/4G mobile network deployed over a major European country. Through correlation and clustering analyses, our study unveils a strong heterogeneity in the demand for different mobile services, both in time and space. In particular, we show that: (i) somehow surprisingly, almost all considered services exhibit quite different temporal usage patterns; (ii) in contrast to such temporal behavior, spatial patterns are fairly uniform across all services; (iii) when looking at usage patterns at different locations, the average traffic volume per user is dependent on the urbanization level, yet its temporal dynamics are not. Our findings do not only have sociological implications, but are also relevant to the orchestration of network resources. This research work has been performed in the framework of the H2020-ICT-2014-2 project 5G NORMA (Grant Agreement No. 671584).

Objective: To evaluate pulmonary function and functional capacity in children and adolescents with sickle cell disease. Methods: This was a cross-sectional study involving 70 children and adolescents (8-15 years of age) with sickle cell disease who underwent pulmonary function tests (spirometry) and functional capacity testing (six-minute walk test). The results of the pulmonary function tests were compared with variables related to the severity of sickle cell disease and history of asthma and of acute chest syndrome. Results: Of the 64 patients who underwent spirometry, 15 (23.4%) showed abnormal results: restrictive lung disease, in 8 (12.5%); and obstructive lung disease, in 7 (10.9%). Of the 69 patients who underwent the six-minute walk test, 18 (26.1%) showed abnormal results regarding the six-minute walk distance as a percentage of the predicted value for age, and there was a ≥ 3% decrease in SpO2 in 36 patients (52.2%). Abnormal pulmonary function was not significantly associated with any of the other variables studied, except for hypoxemia and restrictive lung disease. Conclusions: In this sample of children and adolescents with sickle cell disease, there was a significant prevalence of abnormal pulmonary function. The high prevalence of respiratory disorders suggests the need for a closer look at the lung function of this population, in childhood and thereafter. RESUMO Objetivo: Avaliar a função pulmonar e a capacidade funcional em crianças e adolescentes com doença falciforme. Métodos: Estudo transversal com 70 crianças e adolescentes com doença falciforme (8-15 anos), submetidos a testes de função respiratória (espirometria) e de capacidade funcional (teste de caminhada de seis minutos). Os resultados da avaliação da função pulmonar foram comparados com variáveis relacionadas à gravidade da doença falciforme e à presença de história de asma e de síndrome torácica aguda. Resultados: Dos 64 pacientes submetidos à espirometria, 15 (23,4%) apresentaram resultados alterados: distúrbio ventilatório restritivo, em 8; (12,5%) e distúrbio respiratório obstrutivo, em 7 (10,9%). Dos 69 pacientes submetidos ao teste de caminhada de seis minutos, 18 (26,1%) apresentaram resultados alterados na distância em % do previsto para a idade, e houve uma queda ≥ 3% na SpO2 em 36 (52,2%) dos pacientes. Não houve associações significativas entre função pulmonar alterada e as outras variáveis analisadas, exceto para hipoxemia e distúrbio ventilatório restritivo. Conclusões: Observou-se uma significativa prevalência de alterações na função pulmonar nesta amostra de crianças e adolescentes com doença falciforme. A elevada prevalência de distúrbios ventilatórios sugere a necessidade de um olhar mais atento à função pulmonar desde a infância nessa população.

Use of breast magnetic resonance imaging (MRI) to detect breast cancer has generated significant debate. We analyze the role of breast MRI in the detection of additional disease and the need to perform additional biopsies in early breast carcinoma patients. In addition, we correlate the detection of new foci with tumor pathological features.Early breast carcinoma patients that had undergone an MRI as well as a mammography as diagnostic procedures were included in the study. The following pathologic features were studied: carcinoma type, histological grade, estrogen receptors (ER), progesterone receptors (PR), HER2 and Ki67. Univariate analysis was conducted to ascertain significant correlation among detection of new foci and each of the tumor pathological features.Data from 98 patients have been analyzed: median age 49 years (range 35-79); carcinoma type: (a) infiltrative ductal carcinoma (n = 73, 74 %), (b) infiltrative lobular cancer (n = 12, 12 %), (c) ductal carcinoma in situ (n = 6, 6 %); amplified HER2 (n = 18, 18 %); grade III (n = 33, 33 %); Ki67 ≥ 25 % (n = 33, 33.67 %); positive ER and PR (n = 79, 80 %); triple negative tumors (n = 8, 8 %). MRI detected additional disease in 38 cases (39.58 %), and 20 led to an additional biopsy (20.4 %). Thirty-eight patients (39 %) underwent mastectomy. We found a statistically significant correlation between new foci in MRI and high Ki67 ≥ 25 % (p0.005). No other statistically significant correlation was established.MRI detected additional disease in 39 % cases, requiring an additional biopsy 20 %. Tumors with high proliferative index were significantly correlated with the detection of new foci in MRI.

The process of Chemistry teach-learning is being object of concern to educators of the area since a long time. This concern has stimulated a search for improvements in the quality of education, what it evidences the number of projects that aim expressive changes in the pedagogical practices. In this direction, the approach of chemical contents with the subject “Phosphate Fertilizers” had as objective show for the students the other side of Chemistry, with intention to promote a differentiated education, arousing in the student the curiosity and motivation. The development of the project was through of a mini course, with six meeting of 4 hours each one, where has been presented to the students expositive and experimental classes, through the use of differentiated pedagogical techniques, how talks, visits techniques, pedagogical games among others. During the meetings, was possible to realize that the students feel more motivated and enthusiastic when the teacher promotes differentiated activities, these feelings are essentials for a significant learning. So, it was possible to conclude that the use of the subject created in the students an ampler vision of Chemistry that involves the soil and other important points that allowed the growth of the students as citizen.

Two data fusion strategies (high- and mid-level) combined with a multivariate classification approach (Soft Independent Modelling of Class Analogy, SIMCA) have been applied to take advantage of the synergistic effect of the information obtained from two spectroscopic techniques: FT-Raman and NIR. Mid-level data fusion consists of merging some of the previous selected variables from the spectra obtained from each spectroscopic technique and then applying the classification technique. High-level data fusion combines the SIMCA classification results obtained individually from each spectroscopic technique. Of the possible ways to make the necessary combinations, we decided to use fuzzy aggregation connective operators. As a case study, we considered the possible adulteration of hazelnut paste with almond. Using the two-class SIMCA approach, class 1 consisted of unadulterated hazelnut samples and class 2 of samples adulterated with almond. Models performance was also studied with samples adulterated with chickpea. The results show that data fusion is an effective strategy since the performance parameters are better than the individual ones: sensitivity and specificity values between 75% and 100% for the individual techniques and between 96-100% and 88-100% for the mid- and high-level data fusion strategies, respectively.

The present investigation objectified analyzes the limits and possibilities formatted of webquest to introduce the research on teaching formation on chemistry. For so, we started from the research choiced from Demo (1997; 2002) Maldaner (2000) and Galiazzi (2003) that pointed, between others characteristics of research job, the new elaboration of knowledge by the allowing that by himself assume how author of your formation, I mean, it´s capable of build knowledge from own initiating, learning how to learn. The investigation occur on discipline context of supervised curricular probation in chemistry IV from a licenciature in chemistry from Itumbiara GO. Opted for one investigation of qualitative kind, ruled on case studying, for develop on natural scene, reach on a descriptive scenery, reach on a descriptive data, focus on process and the variables that interfere on the research way. To catch and register the data were used the questionnaire, recording in audio and camp notes. The analyses shows that the elaboration of webquest become possible the development of capacities ah abilities needed to the research: the search and the new construction of the knowledge. But the academician present difficult to the decisions and dependence to models and standard to be followed. The result indicate the necessity of this abort during the initial formation of the teacher for the same become better your practice on research to your context and become a researcher. The webquest produced were changed, seen that some groups showed bigger interest and dedication, once that the work with the research need a give a bigger time to study and base. How consequence, the webquest sows the meaning potency on research teaching formation to become possible the use of informations available on building of knowledge from an emancipated form. Meanwhile we should detach that this is a process that once mobilized on initial formation, must be extend during all the existence and teacher practice with different formative strategy. A presente investigação objetivou analisar os limites e possibilidades formativas da webquest para introduzir a pesquisa na formação docente em Química. Para tanto, partimos da concepção de pesquisa adotada por Demo (1997; 2002), Maldaner (2000) e Galiazzi (2003) que apontam, entre outras características do trabalho com a pesquisa, a (re)elaboração do conhecimento pelo licenciando que se assume como autor de sua formação, ou seja, é capaz de construir conhecimento por iniciativa própria, aprendendo a aprender. A investigação ocorreu no contexto da disciplina de Estágio Curricular Supervisionado em Química IV de um curso de licenciatura em Química de Itumbiara-GO. Optou-se por uma abordagem de investigação do tipo qualitativa, pautada no estudo de caso, por se desenvolver em um cenário natural, rico em dados descritivos, focando o processo e as variáveis que interferem no caminhar da pesquisa. Para busca e registro dos dados utilizou-se de questionários, gravações em áudio e notas de campo. As análises revelaram que a elaboração da webquest possibilitou o desenvolvimento de capacidades e habilidades necessárias à pesquisa: a busca e a (re)construção do conhecimento. Porém os acadêmicos apresentaram dificuldades para a tomada de decisões e dependência a modelos e padrões a serem seguidos. Os resultados indiciam a necessidade dessa abordagem durante a formação inicial do professor para que o mesmo agregue a prática da pesquisa ao seu contexto e se constitua como pesquisador. As webquests produzidas foram diferenciadas, visto que alguns grupos demonstraram maior interesse e dedicação, uma vez que o trabalho com a pesquisa requer uma disponibilidade maior de tempo para o estudo e fundamentação. Por conseguinte, a webquest apresenta um potencial significativo na formação do docente pesquisador por possibilitar a utilização das informações disponíveis na construção dos saberes de forma emancipada. Entretanto devemos destacar que esse é um processo que uma vez mobilizado na formação inicial, deve se estender durante toda a vivência e prática do professor com diferentes estratégias formativas. Mestre em Química

e11517 Background: Aim:To study the relation of positive(pos) SN and mMTS or ITC in negative(neg) SN at surgery with tumor pathological(pathol) features (grade;carcinoma type;Ki67;Her2;hormonal status;tumor size). Methods: Cohort of 271 patients with clinically and ultrasonographically node-neg pT1-2 breast cancer and high grade or extended intraductal who received breast cancer surgery with SN biopsy alone in the last 2 years was studied. No previous chemotherapy was administered. Hormone(ER/RP) and Ki67 status were analyzed by inmunohistochemistry(IHC) while HER2 by FISH. Presence of mMTS and ITC were studied by IHC(AE1-AE3). Statistical analysis was conducted to ascertain significant relation among pos-SN or detection of mMTS/ITC in neg-SN and each of the tumor pathol features (test U-Mann-Whitney). Results: Data from 263 patients were analyzed (28 pos-SN;235 neg-SN;8 technique failures). Carcinoma type: Ductal (158;60%);lobular (46;17.5%);in-situ(53;20.2%);others(6;2.3%). Infiltrative cancer:(56% grade 2;20% grade 3;10% Her2+;9% triple-neg;81% pos-RE/RP). In-situ:(28% grade 2;64% grade 3;39% Her2+;55% pos-RE/RP). Mean pathological size 16.2mm(1-118) for infiltrative tumor and 20.1mm(1-90) for in-situ. Mean of SN found at surgery 1.98(1-5). In neg-SN, 35 cases with mMTS(14;6.2%) or ITC(21;9.2%) were found. No statistically significant relation was established between the finding of mMTS or ITC and any of the pathol features at in-situ or infiltrative tumors. In pos-SN a statistically significant relation between pos-SN and pathol size was found (p=0.003; mean pathol size 20.16mm and 15.69mm for pos and neg-SN). Conclusions: A significant relation was established with pathol size and pos-SN. In patients with neg-SN no significant relationship was found between detection of mMTS or ITC and the pathol features of the tumor.

Introduccion El neuroblastoma (NB) es el tumor abdominal mas frecuente de la infancia y tipicamente aparece en los primeros anos de la vida. Su comportamiento clinico y biologico es peculiar, dado que puede regresar espontaneamente o mostrar una alta agresividad. Las recidivas tardias consideradas como aquellas que se producen mas alla de los 5 anos del diagnostico son muy raras. En este sentido, presentamos el caso de un paciente que sufrio una recidiva de NB 16 anos mas tarde del diagnostico primitivo. Reflexionamos sobre la incertidumbre pronostica. Caso clinico Paciente varon de 19 anos que ingresa para estudio de tumoracion laterocervical y axilar izquierda de una semana de evolucion, sin signos de infeccion aparente. La exploracion no mostro otros hallazgos patologicos. A los 3 anos de vida fue diagnosticado de neuroblastoma abdominal suprarrenal izquierdo estadio 3. Fue remitido a hospital de referencia para tratamiento consiguiendose la remision completa y mantenida. La TC cervical realizada al ingreso en nuestro centro mostro un conglomerado adenopatico supraclavicular e infraclavicular izquierdo. Con la sospecha de un segundo tumor maligno, la masa fue biopsiada siendo el diagnostico histologico compatible con neuroblastoma. En la analitica destaco: LDH: 596 U/l; ferritina: 347 ng/ml; enolasa neuronal especifica: 85,5 ng/ml; catecolaminas en orina de 24 h elevadas: acido vanilmandelico 12,6 mg, acido homovanilico 52,5 mg y dopamina 16.168 μg. El estudio de extension tumoral incluyo: TC toracoabdominal: masa heterogenea en zona suprarrenal izquierda de 10 × 5 × 5 cm junto a grandes vasos que se extiende retrocruralmente a mediastino posterior. Gammagrafia con MIBG: captacion patologica del radioisotopo a nivel suprarrenal izquierdo, laterocervical izquierdo y subclavia izquierda. Biopsia de medula osea: no evidencia de metastasis. Con el diagnostico de recidiva local y metastasica de NB se realiza una segunda biopsia de la tumoracion cervical para estudio genetico, observandose ganancia de N-myc y delecion del 1p. El paciente se incluyo en el protocolo SIOP para neuroblastomas de alto riesgo (HR-NBL-1). Posteriormente ha recibido auto-TPH y tratamiento con cis-retinoico. Tras 5 meses de seguimiento sin tratamiento ha presentado nueva recidiva local en region axilar y abdomen. Actualmente se encuentra en tratamiento con topotecan y temozolamida. Comentarios El caso presentado nos hace reflexionar sobre: 1. Cuanto tiempo esperar antes de considerar curado un NB? El curso indolente de la enfermedad ha hecho que algunos autores utilicen el termino de NB cronico para referirse a los casos de recidivas tardias. A pesar de su infrecuencia es obvia la necesidad de recomendar un seguimiento prolongado en pacientes con NB. 2. Que desenlace debe esperarse? La mayoria de los casos provienen de enfermedad primaria avanzada o metastasica y la mortalidad comunicada es alta. A pesar de la agresividad del tratamiento en la segunda recidiva no hemos podido evitar la tercera recidiva, lo que orienta hacia un tumor resistente a la quimioterapia.

Introduction Human epidermal growth factor receptor 2 (HER2) amplification is frequent in ductal carcinoma in situ (DCIS) of the breast and is associated with poorly differentiated tumors and adverse prognosis features. This study aimed to determine the molecular effects of the HER2 inhibitor lapatinib in patients with HER2 positive DCIS. Methods Patients with HER2 positive DCIS received 1,500 mg daily of lapatinib for four consecutive weeks prior to surgical resection. Magnetic resonance imaging (MRI) was used to determine changes in tumor volume. The molecular effects of lapatinib on HER2 signaling (PI3K/AKT and RAS/MAPK pathways), cell proliferation (Ki67 and p27) and apoptosis (TUNEL) were determined in pre and post-lapatinib treatment samples. Results A total of 20 patients were included. Lapatinib was well tolerated with only minor and transient side effects. The agent effectively modulated HER2 signaling decreasing significantly pHER2 and pERK1 expression, together with a decrease in tumor size evaluated by MRI. There was no evidence of changes in Ki67. Conclusions Four weeks of neoadjuvant lapatinib in patients with HER2-positive DCIS resulted in inhibition of HER2 and RAS/MAPK signaling pathway. Trial registration 2008-004492-21 (Registered June 25th 2008). Electronic supplementary material The online version of this article (doi:10.1186/bcr3695) contains supplementary material, which is available to authorized users.