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41 results on '"Aujard, Isabelle"'

1. Fluorescence to measure light intensity

Author

Lahlou, Aliénor, Tehrani, Hessam Sepasi, Coghill, Ian, Shpinov, Yuriy, Mandal, Mrinal, Plamont, Marie-Aude, Aujard, Isabelle, Niu, Yuxi, Nedbal, Ladislav, Lazár, Dusan, Mahou, Pierre, Supatto, Willy, Beaurepaire, Emmanuel, Eisenmann, Isabelle, Desprat, Nicolas, Croquette, Vincent, Jeanneret, Raphaël, Le Saux, Thomas, and Jullien, Ludovic

Published
2023
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2. Control of Protein Activity and Gene Expression by Cyclofen‐OH Uncaging

Author

Zhang, Weiting, Hamouri, Fatima, Feng, Zhiping, Aujard, Isabelle, Ducos, Bertrand, Ye, Shixin, Weiss, Shimon, Volovitch, Michel, Vriz, Sophie, Jullien, Ludovic, and Bensimon, David

Subjects
Genetics, Biotechnology, Generic health relevance, Animals, Gene Expression Regulation, Green Fluorescent Proteins, Humans, Ligands, Optogenetics, Polycyclic Compounds, Receptors, Estrogen, Recombinant Fusion Proteins, caged compounds, cyclofen-OH, gene expression, optogenetics, photolysis, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Organic Chemistry
Abstract

The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.

Published
2018

3. Optical Control of Tumor Induction in the Zebrafish.

Author

Feng, Zhiping, Nam, Suzy, Hamouri, Fatima, Aujard, Isabelle, Ducos, Bertrand, Vriz, Sophie, Volovitch, Michel, Jullien, Ludovic, Lin, Shuo, Weiss, Shimon, and Bensimon, David

Subjects
Animals, Zebrafish, Humans, Neoplasms, Cell Transformation, Neoplastic, Disease Models, Animal, Gene Expression Regulation, Neoplastic, Mutation, Oncogenes, Proto-Oncogene Proteins p21(ras), Transcriptional Activation, Biomarkers, Tumor, Cell Transformation, Neoplastic, Disease Models, Animal, Gene Expression Regulation, Biomarkers, Tumor, Cancer, Genetics, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, Generic Health Relevance, Biochemistry and Cell Biology, Other Physical Sciences
Abstract

The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells.

Published
2017

9. Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging

Author

Benaissa, Hela, primary, Ounoughi, Karim, additional, Aujard, Isabelle, additional, Fischer, Evelyne, additional, Goïame, Rosette, additional, Nguyen, Julie, additional, Tebo, Alison G., additional, Li, Chenge, additional, Saux, Thomas Le, additional, Danglot, Lydia, additional, Pietrancosta, Nicolas, additional, Morin, Xavier, additional, Jullien, Ludovic, additional, and Gautier, Arnaud, additional

Published
2021
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10. Synthesis and properties of water-soluble gold colloids covalently derivatized with neutral polymer monolayers

Author

Mangeney, Claire, Ferrage, Fabien, Aujard, Isabelle, Marchi-Artzner, Valerie, Jullien, Ludovic, Ouari, Olivier, Rekai, El Djouhar, Laschewsky, Andre, Vikholm, Inger, and Sadowski, Janusz, W.

Subjects
Monomolecular films -- Research, Colloids -- Chemical properties, Gold compounds -- Chemical properties, Water-soluble polymers -- Structure, Chemistry
Abstract

The study presents a report on gold colloids, which are covalently derivatized with hydrophilic polymer brushes. A description on the preparation and properties of gold colloids is provided.

Published
2002

13. Fluorogenic Probing of Membrane Protein Trafficking

Author

Li, Chenge, primary, Mourton, Aurélien, additional, Plamont, Marie-Aude, additional, Rodrigues, Vanessa, additional, Aujard, Isabelle, additional, Volovitch, Michel, additional, Le Saux, Thomas, additional, Perez, Franck, additional, Vriz, Sophie, additional, Jullien, Ludovic, additional, Joliot, Alain, additional, and Gautier, Arnaud, additional

Published
2018
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15. Dynamic multicolor protein labeling in living cells

Author

Li, Chenge, primary, Plamont, Marie-Aude, additional, Sladitschek, Hanna L., additional, Rodrigues, Vanessa, additional, Aujard, Isabelle, additional, Neveu, Pierre, additional, Le Saux, Thomas, additional, Jullien, Ludovic, additional, and Gautier, Arnaud, additional

Published
2017
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19. Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain

Author

Rampon, Christine, primary, Gauron, Carole, additional, Lin, Thibault, additional, Meda, Francesca, additional, Dupont, Edmond, additional, Cosson, Adrien, additional, Ipendey, Eliane, additional, Frerot, Alice, additional, Aujard, Isabelle, additional, Le Saux, Thomas, additional, Bensimon, David, additional, Jullien, Ludovic, additional, Volovitch, Michel, additional, Vriz, Sophie, additional, and Joliot, Alain, additional

Published
2015
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21. Two-Photon Uncaging with Fluorescence Reporting: Evaluation of the o-Hydroxycinnamic Platform

Author

Gagey, Nathalie, Neveu, Pierre, Benbrahim, Chouaha, Goetz, Bernard, Aujard, Isabelle, Baudin, Jean-Bernard, Jullien, Ludovic, Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Statistique de l'ENS (LPS), Université Paris Diderot - Paris 7 (UPD7)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Biomolécules : synthèse, structure et mode d'action (UMR 8642) (BIOSYMA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC), Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry
Abstract

International audience; This paper evaluates the o-hydroxycinnamic platform for designing efficient caging groups with fluorescence reporting upon one- and two-photon excitation. The model cinnamates are easily prepared in one step by coupling commercial or readily available synthons. They exhibit a large one-photon absorption that can be tuned in the near-UV range. Uncaging after one-photon excitation was investigated by 1H NMR, UV-vis absorption, and steady-state fluorescence emission. In the whole investigated series, the caged substrate is quantitatively released upon photolysis. At the same time, uncaging releases a strongly fluorescent coproduct that can be used as a reporter for quantitative substrate delivery. The quantum yield of double bond photoisomerization leading to uncaging after one-photon absorption mostly lies in the 10% range. Taking advantage of the favorable photophysical properties of the uncaging coproduct, we use a series of techniques based on fluorescence emission to measure the action uncaging cross sections with two-photon excitation of the present cinnamates. Exhibiting values in the 1-10 GM range at 750 nm, they satisfactorily compare with the most efficient caging groups reported to date. Noticeably, the uncaging behavior with two-photon excitation is retained in vivo as suggested by the results observed in living zebrafish embryos. Reliable structure property relationships were extracted from analysis of the present collected data. In particular, the careful kinetic analysis allows us to discuss the relevance of the o-hydroxycinnamic platform for diverse caging applications with one- and two-photon excitation.

Published
2007

25. A Blue-Absorbing Photolabile Protecting Group for in Vivo Chromatically Orthogonal Photoactivation

Author

Fournier, Ludovic, primary, Gauron, Carole, additional, Xu, Lijun, additional, Aujard, Isabelle, additional, Le Saux, Thomas, additional, Gagey-Eilstein, Nathalie, additional, Maurin, Sylvie, additional, Dubruille, Sylvie, additional, Baudin, Jean-Bernard, additional, Bensimon, David, additional, Volovitch, Michel, additional, Vriz, Sophie, additional, and Jullien, Ludovic, additional

Published
2013
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27. Photoactivation of the CreERT2 Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution

Author

Sinha, Deepak Kumar, primary, Neveu, Pierre, additional, Gagey, Nathalie, additional, Aujard, Isabelle, additional, Le Saux, Thomas, additional, Rampon, Christine, additional, Gauron, Carole, additional, Kawakami, Koichi, additional, Leucht, Christoph, additional, Bally-Cuif, Laure, additional, Volovitch, Michel, additional, Bensimon, David, additional, Jullien, Ludovic, additional, and Vriz, Sophie, additional

Published
2010
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29. Photocontrol of Protein Activity in Cultured Cells and Zebrafish with One‐ and Two‐Photon Illumination

Author

Sinha, Deepak Kumar, primary, Neveu, Pierre, additional, Gagey, Nathalie, additional, Aujard, Isabelle, additional, Benbrahim‐Bouzidi, Chouaha, additional, Le Saux, Thomas, additional, Rampon, Christine, additional, Gauron, Carole, additional, Goetz, Bernard, additional, Dubruille, Sylvie, additional, Baaden, Marc, additional, Volovitch, Michel, additional, Bensimon, David, additional, Vriz, Sophie, additional, and Jullien, Ludovic, additional

Published
2010
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30. Photo-Control of Protein Activity in a Single Cell of a Live Organisim

Author

Sinha, Deepak K., primary, Neveu, Pierre, additional, Gagey, Nathalie, additional, Aujard, Isabelle, additional, Benbrahim-Bouzidi, Chouaha, additional, Le Saux, Thomas, additional, Rampon, Christine, additional, Gauron, Carole, additional, Goetz, Bernard, additional, Dubruille, Sylvie, additional, Bally-Cuif, Laure, additional, Volovitch, Michel, additional, Bensimon, David, additional, Jullien, Ludovic, additional, and Vriz, Sophie, additional

Published
2010
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35. Tetrahedral onsager crosses for solubility improvement and crystallization bypass

Author

Aujard, Isabelle, Baltaze, Jean-Pierre, Baudin, Jean-Bernard, Cogne, Emmanuelle, Ferrage, Fabien, Jullien, Ludovic, Perez, Eric, Prevost, Valery, Qian, Lin Mao, and Ruel, Odile

Subjects
Molecular crystals -- Research, Solubility -- Research, Chemistry
Abstract

Research exploring methods of improving solubility of organic concave shape molecules is presented. Particular attention is given to the method of tetrahedral onsager crosses in the process of conjugating molecules and to the method ultraviolet absorption.

Published
2001

39. A Blue-Absorbing Photolabile Protecting Group for in VivoChromatically Orthogonal Photoactivation

Author

Fournier, Ludovic, Gauron, Carole, Xu, Lijun, Aujard, Isabelle, Le Saux, Thomas, Gagey-Eilstein, Nathalie, Maurin, Sylvie, Dubruille, Sylvie, Baudin, Jean-Bernard, Bensimon, David, Volovitch, Michel, Vriz, Sophie, and Jullien, Ludovic

Abstract

The small and synthetically easily accessible 7-diethylamino-4-thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section for uncaging in the 470–500 nm wavelength range and a low light absorption between 350 and 400 nm. These attractive features have been implemented in living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development with UV and blue-cyan light sources, respectively.

Published
2013
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40. Photoactivation of the CreERT2Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution

Author

Sinha, Deepak Kumar, Neveu, Pierre, Gagey, Nathalie, Aujard, Isabelle, Le Saux, Thomas, Rampon, Christine, Gauron, Carole, Kawakami, Koichi, Leucht, Christoph, Bally-Cuif, Laure, Volovitch, Michel, Bensimon, David, Jullien, Ludovic, and Vriz, Sophie

Abstract

AbstractWe implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreERT2fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).

Published
2010
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