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A Blue-Absorbing Photolabile Protecting Group for in VivoChromatically Orthogonal Photoactivation

Authors :
Fournier, Ludovic
Gauron, Carole
Xu, Lijun
Aujard, Isabelle
Le Saux, Thomas
Gagey-Eilstein, Nathalie
Maurin, Sylvie
Dubruille, Sylvie
Baudin, Jean-Bernard
Bensimon, David
Volovitch, Michel
Vriz, Sophie
Jullien, Ludovic
Source :
ACS Chemical Biology; July 2013, Vol. 8 Issue: 7 p1528-1536, 9p
Publication Year :
2013

Abstract

The small and synthetically easily accessible 7-diethylamino-4-thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section for uncaging in the 470–500 nm wavelength range and a low light absorption between 350 and 400 nm. These attractive features have been implemented in living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development with UV and blue-cyan light sources, respectively.

Details

Language :
English
ISSN :
15548929 and 15548937
Volume :
8
Issue :
7
Database :
Supplemental Index
Journal :
ACS Chemical Biology
Publication Type :
Periodical
Accession number :
ejs30378029
Full Text :
https://doi.org/10.1021/cb400178m