Usefulness of a Non-Streptavidin Bead Technology to Overcome Biotin Interference: Proof of Principle with 25-OH Vitamin D, TSH, and FT4.

To the Editor: Biotin interference has become a matter of concern with immunoassays using the biotin-streptavidin immobilization system because of the risk of falsely low and falsely high results observed in sandwich and competitive assays, respectively (1–4). Therefore, the identification of these interferences is necessary to avoid misleading diagnoses and potential adverse consequences for patients.[...]