Back to Search
Start Over
Increased cucumber salt tolerance by grafting on pumpkin rootstock and after application of calcium
- Source :
- Biologia Plantarum, Vol 58, Iss 1, Pp 179-184 (2014)
- Publication Year :
- 2014
- Publisher :
- Institute of Experimental Botany of the Czech Academy of Sciences, 2014.
-
Abstract
- Self-grafted and pumpkin rootstock-grafted cucumber plants were subjected to the following four treatments: 1) aerated nutrient solution alone (control), 2) nutrient solution with 10 mM Ca(NO3)2 (Ca), 3) nutrient solution with 90 mM NaCl (NaCl), and 4) nutrient solution with 90 mM NaCl + 10 mM Ca(NO3)2 (NaCl+Ca). The NaCl treatment decreased the plant dry mass and content of Ca2+ and K+ but increased the Na+ content in roots and shoots. Smaller changes were observed in pumpkin rootstock-grafted plants. Supplementary Ca(NO3)2 ameliorated the negative effects of NaCl on plant dry mass, relative growth rate (RGR), as well as Ca2+, K+, and Na+ content especially for pumpkin rootstock-grafted plants. Supplementary Ca(NO3)2 distinctly stimulated the plasma membrane (PM) H+-ATPase activity which supplies the energy to remove excess Na+ from the cells. The expressions of gene encoding PM H+-ATPases (PMA) and gene encoding a PM Na+/H+ antiporter (SOS1) were up-regulated when Ca(NO3)2 was applied. The pumpkin rootstock-grafted plants had higher PM H+-ATPase activity as well as higher PMA and SOS1 expressions than the self-grafted plants under NaCl + Ca treatment. Therefore, the addition of Ca2+ in combination with pumpkin rootstock grafting is a powerful way to increase cucumber salt tolerance.
Details
- Language :
- English
- ISSN :
- 15738264
- Volume :
- 58
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Biologia Plantarum
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.42c98e77b70f4067b5ebd0a1b2a9ca9b
- Document Type :
- article
- Full Text :
- https://doi.org/10.1007/s10535-013-0349-6