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Global Phenolic Composition and Antioxidant Capacity of Extracts from the Endophytic Fungi Cophinforma mamane with Potential Use in Food Systems: The Effects of Time, Temperature, and Solvent on the Extraction Process

Authors :
Raiana S. Gurgel
Dorothy I. M. Pereira
Bárbara Martins
Lucas S. Falcão
Caroline D. Lacerda
Cláudia M. B. Neves
António F. Pinto
António M. Jordão
Patrícia M. Albuquerque
Source :
Applied Sciences, Vol 14, Iss 19, p 8784 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Endophytic fungi are promising producers of phenolic compounds; however, the process of extracting these molecules from the culture medium is a crucial step for obtaining these metabolites. In this context, the best conditions for extracting phenolic compounds produced by Cophinforma mamane CF2-13, isolated from an Amazonian plant, were evaluated. The fungus was cultivated in a liquid medium to obtain its metabolites. The effects of the solvent, time, and temperature were investigated, as well as the use of ultrasound. The total phenolics and the antioxidant activity were assessed to define the best extraction conditions. The optimized extract was chemically characterized and used to analyze its potential in protecting olive oil from lipid oxidation. The best results were found using ethyl acetate at 20 °C for 2 h. These conditions led to 61.61 mg GAEq/g of extract of total phenolics, and an antioxidant capacity of 0.47, 0.45, and 0.29 mmol TEq/g of extract using the DPPH, ABTS, and FRAP methods, respectively. In addition, the optimized extract presented caffeic acid and was able to delay the lipid oxidation of olive oil. The potential of endophytic fungi to produce phenolic compounds is promising for the food industry since natural bioactive compounds have many benefits for human health.

Details

Language :
English
ISSN :
20763417
Volume :
14
Issue :
19
Database :
Directory of Open Access Journals
Journal :
Applied Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.150414c3ca49f484af791db0589a93
Document Type :
article
Full Text :
https://doi.org/10.3390/app14198784