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Effect of Dietary Protein Source on the Activity of Polysomal Ornithine Decarboxylase Messenger RNA in Rat Liver
- Source :
- The Journal of Nutrition. 117:1801-1804
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- To pursue the mechanism underlying dietary induction of ornithine decarboxylase (ODC) activity in rat liver, changes in free-polysome-associated messenger RNA (mRNA) activity for the enzyme were studied in rats fed diets containing either casein or zein. The enzyme activity increased 50- to 100-fold in 4 h after feeding a 50% casein diet. Changes in the amount of immunoreactive ODC protein roughly paralleled changes in the enzyme activity. Dietary casein was found to cause a several fold increase in polysomal mRNA activity coding for this enzyme, which preceded the increase in catalytic activity. Therefore, the induction of hepatic ODC by casein feeding is due partly to an increase in the activity of its mRNA associated with free polysomes and partly to some translational and/or posttranslational control. On the other hand, feeding the zein diet hardly increased ODC activity, immunoreactive ODC protein or free-polysomal ODC-mRNA activity. This indicates that adequate supply and balance of amino acids are required both for the increase in polysome-associated ODC-mRNA activity and for the translational and/or posttranslational mechanism(s) during the induction of hepatic ODC activity.
- Subjects :
- Male
genetic structures
Medicine (miscellaneous)
Biology
Ornithine Decarboxylase
Ornithine decarboxylase
Casein
Polysome
Gene expression
Protein biosynthesis
Animals
RNA, Messenger
Ornithine decarboxylase antizyme
Messenger RNA
Nutrition and Dietetics
fungi
Rats, Inbred Strains
Enzyme assay
Rats
Liver
Biochemistry
RNA, Ribosomal
Enzyme Induction
Polyribosomes
biology.protein
Dietary Proteins
Subjects
Details
- ISSN :
- 00223166
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- The Journal of Nutrition
- Accession number :
- edsair.doi.dedup.....d35fb9167f6cd65304e8b568a62c482d
- Full Text :
- https://doi.org/10.1093/jn/117.10.1801