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Chemical Composition and Biotechnological Properties of a Polysaccharide from the Peels and Antioxidative Content from the Pulp of Passiflora liguralis Fruits
- Source :
- Journal of agricultural and food chemistry 55 (2007): 7427–7433. doi:10.1021/jf0704615, info:cnr-pdr/source/autori:Tommonaro G; Segura Rodriguez CS; Santillana M; Immirzi B; De Prisco R; Nicolaus B; Poli A./titolo:Chemical Composition and Biotechnological Properties of a Polysaccharide from the Peels and Antioxidative Content from the Pulp of Passiflora liguralis Fruits/doi:10.1021%2Fjf0704615/rivista:Journal of agricultural and food chemistry/anno:2007/pagina_da:7427/pagina_a:7433/intervallo_pagine:7427–7433/volume:55
- Publication Year :
- 2007
- Publisher :
- American Chemical Society (ACS), 2007.
-
Abstract
- A new polysaccharide with a high molecular weight (greater than 1 x 106 Da) was extracted and characterized from the peels of Passiflora liguralis (granadilla) fruits. Chemical composition of the biopolymer, performed by using a high pressure anion exchange-pulsed amperometric detector (HPAE-PAD), showed the presence of six different sugar residues: xylose, glucose, galactose, galactosamine, an unknown component, and fucose in the relative ratio of 1:0.5:0.2:0.06:0.05:trace. The optical rotation of this xyloglucan was [alpha](D)(25) degrees C = -186.42 (concentration of 1.4 mg/mL of H(2)O), and the viscosity was dependent on the concentration and pH, showing a maximum value of 1.4 eta at a concentration of 3% in distilled water and a maximum value of 7.0 eta in citrate buffer solution. Thermogravimetric analysis indicated that this biopolymer was very stable at high temperatures, showing a degradation temperature at 280 degrees C. The characterization of the polysaccharide was also investigated by spectroscopic methods (1H NMR and IR) pointing out the complexity of this biopolymer and the presence of sugar residues in alpha-manno, alpha-gluco-galacto, and beta-gluco-galacto configurations. The formation of a biodegradable film using this novel xyloglucan was reported, and the anticytotoxic activity of the polysaccharide was studied in a brine shrimp bioassay. Considerable antioxidant activity (Trolox equivalent antioxidant capacity (TEAC) value of 0.32 microM/mg fresh product) was noted in the lipophilic extracts of Passiflora liguralis fruits, indicating, in this fruit, an alternative source of bioactive compounds.
- Subjects :
- Chemical Phenomena
Trolox equivalent antioxidant capacity
passion fruit
antioxidant activity
Xylose
engineering.material
Polysaccharide
Antioxidants
Fucose
chemistry.chemical_compound
Polysaccharides
Sugar
Glucans
chemistry.chemical_classification
Chemistry, Physical
Passiflora
Chemistry
Food Packaging
General Chemistry
Xyloglucan
biodegradable film
Distilled water
Biochemistry
polysaccharide
Fruit
engineering
Xylans
Passiflora liguralis
Biopolymer
General Agricultural and Biological Sciences
Sesquiterpenes
Biotechnology
Nuclear chemistry
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....adb654a1067f479d4165b6f7432f6741