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MALDI-MS analysis of disaccharide isomers using graphene oxide as MALDI matrix
- Source :
- Food chemistry. 342
- Publication Year :
- 2019
-
Abstract
- Disaccharides are sugars composed of two monosaccharides joined by a glycosidic linkage. The specific properties of a disaccharide depend on the type of the glycosidic linkage and the identity of the two component monosaccharides. In this work, seven disaccharide isomers (gentiobiose, isomaltose, melibiose, lactose, maltose, cellobiose, and sucrose) were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using a graphene oxide matrix. Each disaccharide was identified by its unique cleavage pattern. To determine the feasibility of quantitative analyses based on specific fragment patterns, mixtures of sucrose with cellobiose or maltose were prepared at different ratios and analyzed by MALDI-MS, where a strong linear correlation was observed between the relative peak intensity of the sucrose fragment peak at m/z 185 and the amount of sucrose in the mixture. The calibration curve was successfully applied to obtain the relative amount of maltose and sucrose in four different honey samples.
- Subjects :
- Disaccharide
Cellobiose
Disaccharides
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
0404 agricultural biotechnology
Isomerism
Gentiobiose
Lactose
Melibiose
chemistry.chemical_classification
Chromatography
010401 analytical chemistry
Glycosidic bond
04 agricultural and veterinary sciences
General Medicine
Maltose
Honey
Isomaltose
040401 food science
0104 chemical sciences
chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Graphite
Food Science
Subjects
Details
- ISSN :
- 18737072
- Volume :
- 342
- Database :
- OpenAIRE
- Journal :
- Food chemistry
- Accession number :
- edsair.doi.dedup.....8c222883a80a8a00383d4f2d253b165e