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Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production
- Source :
- Antibiotics, Volume 9, Issue 11
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- This work aimed to evaluate the antimicrobial susceptibility of 87 Salmonella Infantis strains isolated in Italy from 2016 to 2019 along the food chain of broiler meat production and in humans and to determine the genetic profiles of the strains in order to establish a possible correlation with the antimicrobial pattern. All isolates were tested by the disk diffusion method to evaluate antimicrobial susceptibility toward sixteen antimicrobials, and the broth microdilution method was used to confirm extended spectrum &beta<br />lactamase (ESBL) production. PCR and pulsed field gel electrophoresis (PFGE) were applied to characterize ESBL-encoding and AmpC &beta<br />lactamase genes and to analyze the S. Infantis strains genetic profiles respectively. S. Infantis isolates showed high prevalence of resistance, in particular toward nalidixic acid (97.7%), tetracycline (96.5%), sulphamethoxazole/trimethoprim (91%) and cefepime (72.4%). The 80.5% of isolates were ESBL, cefotaxime-resistant, carrying the blaCTX-M1 gene. The most prevalent PFGE profile was XbaI.0126 (35.6%). The remaining strains had a genetic homology from 81% to 97% with the XbaI.0126 profile. The strains belonging to these profiles were isolated from different matrices collected along the broiler food chain independently on the year and from the region and there was no correlation between the PFGE profiles and resistance patterns. We found two ESBL-producing S. Infantis strains with the same XbaI.2621 profile isolated from humans and from poultry feces, not yet reported in Italy. Our findings confirmed the diffusion of ESBL-multi drug resistant (MDR) S. Infantis along the broiler food chain and in humans and underlined the importance of continuous monitoring to control and to reduce the prevalence of this bacterium, applying a global One Health approach.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Veterinary medicine
Nalidixic acid
Tetracycline
Cefepime
030106 microbiology
Salmonella Infantis
Drug resistance
Biology
Biochemistry
Microbiology
antimicrobial susceptibility
03 medical and health sciences
Antibiotic resistance
Pulsed-field gel electrophoresis
medicine
ESBL
genetic profile
PFGE
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Broth microdilution
biochemical phenomena, metabolism, and nutrition
Antimicrobial
bacterial infections and mycoses
030104 developmental biology
Infectious Diseases
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20796382
- Database :
- OpenAIRE
- Journal :
- Antibiotics
- Accession number :
- edsair.doi.dedup.....7e72ec570d7bf3d8e92af6b592670833
- Full Text :
- https://doi.org/10.3390/antibiotics9110814