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Selective Extraction of ω-3 Fatty Acids from Nannochloropsis sp. Using Supercritical CO2 Extraction
- Source :
- Molecules, Volume 24, Issue 13, Molecules, Vol 24, Iss 13, p 2406 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- In this article, microalgae Nannochloropsis sp. was used for fatty acid (FA) extraction, using a supercritical fluid-carbon dioxide (SF-CO2) extraction method. This study investigated the influence of different pre-treatment conditions by varying the grinding speed (200&ndash<br />600 rpm), pre-treatment time (2.5&ndash<br />10 min), and mixing ratio of diatomaceous earth (DE) and Nannochloropsis sp. biomass (0.5&ndash<br />2.0 DE/biomass) on FAs extraction. In addition, the effect of different operating conditions, such as pressure (100&ndash<br />550 bar), temperature (50&ndash<br />75 &deg<br />C), and CO2 flow rate (7.24 and 14.48 g/min) on eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) recovery, was analyzed. Experimental data evidenced that, keeping constant the extraction conditions, the pre-treatment step enhanced the FAs extraction yield up to 3.4 fold, thereby the maximum extracted amount of FAs (61.19 mg/g) was attained with the pre-treatment with a ratio of DE/biomass of 1 at 600 rpm for 5 min. Moreover, by increasing both SF-CO2 pressure and temperature, the selectivity towards EPA was enhanced, while intermediate pressure and lower pressure promoted DHA recovery. The highest amount of extracted EPA, i.e., 5.69 mg/g, corresponding to 15.59%, was obtained at 75 &deg<br />C and 550 bar with a CO2 flow rate of 14.48 g/min, while the maximum amount of extracted DHA, i.e., ~0.12 mg/g, equal to 79.63%, was registered at 50 &deg<br />C and 400 bar with a CO2 flow rate of 14.48 g/min. Moreover, the increased CO2 flow rate from 7.24 to 14.48 g/min enhanced both EPA and DHA recovery.
- Subjects :
- eicosapentaenoic acid
Docosahexaenoic Acids
Pharmaceutical Science
02 engineering and technology
01 natural sciences
fatty acids
Article
Analytical Chemistry
lcsh:QD241-441
lcsh:Organic chemistry
lipid
Drug Discovery
Fatty Acids, Omega-3
Biomass
Physical and Theoretical Chemistry
chemistry.chemical_classification
Nannochloropsis sp
Supercritical carbon dioxide
Chromatography
Supercritical CO2 extraction
010405 organic chemistry
Chemistry
microalgae
Organic Chemistry
Temperature
Fatty acid
Chromatography, Supercritical Fluid
docosahexaenoic acid
Carbon Dioxide
021001 nanoscience & nanotechnology
Eicosapentaenoic acid
6. Clean water
Supercritical fluid
0104 chemical sciences
Volumetric flow rate
Chemistry (miscellaneous)
Docosahexaenoic acid
extraction
Molecular Medicine
nutraceutical
0210 nano-technology
Selectivity
Stramenopiles
Supercritical CO
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....4b2eb9299fab5214bc4d57b1b0f8f7fc