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Cellulolytic potential of probiotic Bacillus Subtilis AMS6 isolated from traditional fermented soybean (Churpi): An in-vitro study with regards to application as an animal feed additive
- Source :
- Microbiological research.
- Publication Year :
- 2015
-
Abstract
- The aim of the present study is to evaluate the probiotic attributes of Bacillus subtilis AMS6 isolated from fermented soybean (Churpi). This isolate exhibited tolerance to low pH (pH 2.0) and bile salt (0.3%), capability to autoaggregate and coaggregate. AMS6 also showed highest antibacterial activity against the pathogenic indicator strain Salmonella enterica typhimurium (MTCC 1252) and susceptibility towards different antibiotics tested. The isolate was effective in inhibiting the adherence of food borne pathogens to Caco-2 epithelial cell lines, and was also found to be non-hemolytic which further strengthen the candidature of the isolate as a potential probiotic. Further studies revealed B. subtilis AMS6 showed cellulolytic activity (0.54±0.05 filter paper units mL(-1)) at 37°C. The isolate was found to hydrolyze carboxymethyl cellulose, filter paper and maize (Zea mays) straw. The maize straw digestion was confirmed by scanning electron microscopy studies. The isolate was able to degrade filter paper within 96h of incubation. A full length cellulase gene of AMS6 was amplified using degenerate primers consisting of 1499 nucleotides. The ORF encoded for a protein of 499 amino acids residues with a predicted molecular mass of 55.04kDa. The amino acids sequence consisted of a glycosyl hydrolase family 5 domain at N-terminal; Glycosyl hydrolase catalytic core and a CBM-3 cellulose binding domain at its C terminal. The study suggests potential probiotic B. subtilis AMS6 as a promising candidate envisaging its application as an animal feed additive for enhanced fiber digestion and gut health of animal.
- Subjects :
- 0301 basic medicine
Paper
Salmonella typhimurium
Cellulase
Bacillus subtilis
Microbiology
Polymerase Chain Reaction
Zea mays
Bacterial Adhesion
law.invention
Bile Acids and Salts
03 medical and health sciences
Probiotic
chemistry.chemical_compound
law
Hydrolase
Antibiosis
Animals
Humans
Glycosyl
Cellulose
biology
Plant Stems
Hydrolysis
Probiotics
Temperature
Epithelial Cells
Sequence Analysis, DNA
Hydrogen-Ion Concentration
Cellulose binding
biology.organism_classification
Animal Feed
030104 developmental biology
chemistry
Biochemistry
biology.protein
Food Additives
Soybeans
Caco-2 Cells
Antibacterial activity
Digestion
Subjects
Details
- ISSN :
- 16180623
- Database :
- OpenAIRE
- Journal :
- Microbiological research
- Accession number :
- edsair.doi.dedup.....47a4d4a6236caf28b98f1974cfc4b042