Effect of Isolation and Crystallization on the Electrophoretic Mobility of β-Lactoglobulin as Determined by Paper Electrophoresis

Summary To study the electrophoretic properties of milk proteins under conditions as nearly similar as possible to those found in milk, a paper strip electrophoresis apparatus was designed which required small volumes of electrolyte and in which electro-osmosis could be substantially reduced. Using this apparatus, it was shown that β -lactoglobulin could be isolated from milk, crystallized, and returned to its native environment without grossly affecting its initial charge density.