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Black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expression via blockage of NF-κB and JNK activation in intestinal epithelial cells.
- Source :
- Inflammation Research; May2011, Vol. 60 Issue 5, p493-500, 8p, 4 Black and White Photographs, 2 Graphs
- Publication Year :
- 2011
-
Abstract
- Objective: The aim of this study was to determine the impact of the black tea polyphenol, theaflavin, on the expression of adhesion molecules and activation of lipopolysaccharide (LPS)-induced innate signaling in rat intestinal epithelial (RIE) cells. Methods: The effect of theaflavin on neutrophil adhesion, expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1, LPS-induced nuclear factor-kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) signaling was examined by neutrophil adhesion assay, RT-PCR, Western blotting, immunofluorescence, and electrophoretic mobility shift assay (EMSA). Results: Theaflavin suppressed adhesion of neutrophils to LPS-stimulated RIE cells. LPS-induced ICAM-1 and VCAM-1 expressions were inhibited by theaflavin. LPS-induced IκBα phosphorylation/degradation and nuclear translocation of NF-κB/p65 were blocked by theaflavin. Also, theaflavin blocked NF-κB DNA-binding activity in EMSA. LPS-induced phosphorylation of JNK was inhibited by theaflavin. Bay11-7082 (a NF-κB inhibitor) and SP600125 (a JNK inhibitor) suppressed the LPS-induced ICAM-1 and VCAM-1 mRNA accumulations. Conclusions: These results indicate that black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expressions through blockage of NF-κB and JNK activation in intestinal epithelial cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- POLYPHENOLS
TEA
CELL adhesion molecules
LABORATORY rats
NF-kappa B
NEUTROPHILS
Subjects
Details
- Language :
- English
- ISSN :
- 10233830
- Volume :
- 60
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Inflammation Research
- Publication Type :
- Academic Journal
- Accession number :
- 59809437
- Full Text :
- https://doi.org/10.1007/s00011-010-0296-z