تولید پروتئین هیدرولیز شده قارچ خوراکی با استفاده از آنزیم آلکالاز بررسی تاثیر زمان هیدرولیز و غلظت پروتئین هیدرولیز شده بر قابلیت آنتی اکسیدانی آن.

Due to concerns about the safety and long-term health of synthetic antioxidants such as BHT and BHA, the use of natural compounds with antioxidant properties, such as bioactive peptides, has attracted the attention of many researchers. The aim of this study was to determine the optimum hydrolysis time of edible mushroom protein ( Agaricus bisporus) with alcalase enzyme to produce protein hydrolyzate with high antioxidant properties. For this study, first edible mushrooms were converted into powder and then was hydrolyzed in different times of 200, 150, 100, 50 and 250 minutes with an enzyme to substrate of 2% at temperature of 50 °C and then appropriate treatment based on antioxidant properties of the produced sample was determined. The results showed that hydrolysis time 200 minutes is suitable to achieve maximum antioxidant properties. Next, the effect of different concentrations (10 to 50 mg/ml) of hydrolyzed protein prepared under optimal conditions on the antioxidant properties of the product compared to ascorbic acid (50 mg/ml) was investigated. In all the tests, the product showed a concentration-dependent behavior and with increasing concentration, the DPPH free radical scavenging, iron ion reduction power and total antioxidant power increased and in concentrations of 40 and 50 mg/ml the antioxidant power was similar to ascorbic acid (50 mg/ml). The results showed that the hydrolyzed protein produced has the potential to be used as a functional ingredient and as an alternative to synthetic antioxidants in food formulations. [ABSTRACT FROM AUTHOR]