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Novel Angiotensin‐Converting Enzyme Inhibitory Peptides Derived from Oncorhynchus mykiss Nebulin: Virtual Screening and In Silico Molecular Docking Study.

Authors :
Yu, Zhipeng
Fan, Yue
Zhao, Wenzhu
Ding, Long
Li, Jianrong
Liu, Jingbo
Source :
Journal of Food Science (John Wiley & Sons, Inc.); Sep2018, Vol. 83 Issue 9, p2375-2383, 9p, 3 Color Photographs, 2 Charts
Publication Year :
2018

Abstract

Abstract: Excessive concentrations of angiotensin‐converting enzyme (ACE) can give rise to high blood pressure, and is harmful to the body. ACE inhibitory peptides from food proteins are considered good sources of function food. However, the preparation of ACE inhibitory peptides by classical method faces many challenges. Three novel ACE inhibitory peptides were identified by in silico methods, and showed potent activity against ACE in vitro. The simulation hydrolysis of nebulin was performed with ExPASy PeptideCutter program. Potential activity, solubility, and absorption, distribution, metabolism, excretion, and toxicity properties of generated peptides were predicted using program online. Molecular docking displayed that EGF, HGR, and VDF were docked into the S<subscript>1</subscript> and S<subscript>2</subscript> pockets of ACE. Meanwhile, Phe and Arg at the C‐terminal enhance ACE affinity. The IC<subscript>50</subscript> values of EGF, HGR, and VDF were 474.65 ± 0.08, 106.21 ± 0.52, and 439.27 ± 0.09 μM, respectively. Three peptides EGF, HGR, and VDF from Oncorhynchus mykiss nebulin were identified, and the molecular mechanism between ACE and peptides was clarified using in silico methods. The results suggested that Oncorhynchus mykiss nebulin would be an attractive raw material of antihypertensive nutraceutical ingredients. Practical Application: This study has shown the potential of Oncorhynchus mykiss nebulin as good sources for producing ACE inhibitory peptides. According to this finding, in silico approach is the feasible way for prediction and identification of food‐derived ACE inhibitory peptides in emerging nutraceutical field. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00221147
Volume :
83
Issue :
9
Database :
Complementary Index
Journal :
Journal of Food Science (John Wiley & Sons, Inc.)
Publication Type :
Academic Journal
Accession number :
131706235
Full Text :
https://doi.org/10.1111/1750-3841.14299