Concentration, detection and discrimination of Bacillus anthracis spores in orange juice using aptamer based surface enhanced Raman spectroscopy.

Herein, we developed an innovative label free platform for concentration, detection and discrimination of Bacillus anthracis spores. Based on aptamer capture and surface enhanced Raman scattering detection, we were able to detect 10⁴ CFU spores spiked in orange juice and discriminate between spores B. anthracis and B. mycoides within 40 min. [ABSTRACT FROM AUTHOR]